AU2008202737A1 - Animal Feed Supplement for the nutritional Enrichment of Animal Produce - Google Patents

Description

21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 16/55 00 0 0 ANIMAL FEED SUPPLEMENT FOR THE NUTRITIONAL ENRICHMENT OF ANIMAL

SPRODUCE

BACKGROUND

c The present invention relates 1o nutritional enrichment of foodstuffs such as, but not limited to pork, C, beef, poultry and the like and more particularly relates to the use of a selection of feed supplements C1 formulated for feeding to animals at predetermined stages of growth and which include increased levels 00 0 of omega 3 long chain fatty acids. The invention further provides feed formulae for animals at various C stages of development and which result in produce having an increased human nutritional quality due to an increased level of long chain omega 3 fatty acids but without taint of the food product. Whilst the supplement and formulae of the present invention are suitable as feed for producing high nutritional quality beef and poultry (in the latter case influencing the nutritional value of poultry meat and eggs) the invention will mainly be described with reference to its application in pig feeding.

PRIOR ART It is long established that there is a direct relationship between animal feed and the quality of food produce from a particular animal. An example of this relationship is evident where fish meal is used in pig feeding with the constitueni ingredient levels of fish meal impacting on the nutritional quality of the food.

The food industry, in recognition of the desirability of lowering of saturated fats in the human diet has paid particular attention to improvement of the nutritional quality of foodstuffs and this begins with the animal feed blends. For instance, in the last ten years the average fat content of pork has been reduced by more than 50% in direct response to consumer demand for low fat diets in view of the links between high cholesterol, heart disease and a high fat diet. Animal products including pork also contain polyunsaturated fatty acids (PUFA). Pork and animal product producers have in recognition of the high nutritional benefits of long chain omega 3 PTJFA and the consequent high consumer demand for foods with high yields of these nutrients have paid particular attention to the sources of these fatty acids and more particularly to ways of fortifying foods with long chain omega 3 PUFA.

1 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 17/55 00 0 0 Omega 3 polyunsaturated fatty acids, like vitamins, are essential to good health but as the body cannot itself manufacture these essential acids and vitamins it must rely on food sources for the requisite supply of these necessities. Foods rich in long chain omega 3 fatty acids are generally not a regular part of the diet so most people are denied the fill potential benefits of the presence of these in the diet.

O Polyunsaturated 'fats are divided into the omega 3 and omega 6 fatty acids both of which the body needs.

00 The effects of long chain omega 3 fatty acids on the body are significant as they are incorporated into every cell, tissue and organ including the heart and lungs, blood vessels, brain and joints.

There are a variety of food sources of long chain omega 3 fatty acids for example, Alpha linolcnic acid (ALA) occurs in oils such as canola, linseed (flaxseed), walnut and soybean as well as in green vegetables. Eicosapentaenoic acid (EPA) can be found in cod liver oil, fish oils fish and other seafoods and even in beef Docosahexaenoic acid (DMA) is found in tuna oil, other fish oils and in breast milk.

While omega 6 fatty acids are also beneficial and necessary for a good diet, the ratio of omega 6 fatty acids to omega 3 should be less than 5:1 and preferably 1:1. An excess of omega 6 acids can negate the eFfect of the long chain omega 3 fatty acids. Long chain Omega 3 fatty acids (EPA and DMA) are derived from fish and are more potent than the short chain omega 3 derived from plants (ALA) as they are taken up directly by the cells. The evidence for the health benefits of DMA and EPA in the diet is nowhere better demonstrated than in Japanese fisherman and Eskimos who both have diets high in fish and a low incidence of heart disease. Omega 3 fatty acids also play a role in slowing the build up of fats on the walls of blood vessels, reducing blood clotting, reducing blood pressure and decreasing arythmia.

There is also evidence that omega 3 fatty acids can reduce risk of cancer and depression and can strengthen the immune system.

The problem for the food industry has been to determine the most effective means of introducing long chain omega 3 fatty acids into the diet without compromising the physical, textural and sensory quality of the food vehicle. Egg producers introduced long chain omega 3 fatty acids into eggs by feeding hens foods such as fish meal which are high in these acids only to discover that the eggs tasted like fish leading to a reduction in consumption of such fat modified eggs. The hens were then fed a unique 2 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 18/55 00 0 0 vegetarian diet of natural foods that are rich in long chain omega 3 fatty acids but there are significant restrictions on how much fatty acid can be introduced due to the problem of sensory taint. This is also Cl the experience in the pork industry where fish meal has been fed to pigs to increase the level of long chain omega 3 fatty acids in consumers of pork, but the neat has also suffered from fishy taint which significantly reduces the level of omega three fatty acids which can be introduced.

e1 Cl O According to conventional wisdom, high intakes by pigs of fishmeal as a source of long chain omega 3 00 PUFA above relatively low levels of 2 5% of the food vehicle results in pork taint manifested by Sdecrease in oleic acid content, increase in iodine value hence oxidizability as well as softening of the Cl carcass fat. Thus there has traditionally in use of PUFA been a necessary compromise between achieving an optimum nutritional value associated with use of PIJFA's while guarding against taint from excessive use.

This has kept the use of PUFA's in pig feed to low levels primarily due to the undesirability of taint. The problems of taint and the desirability of increasing the PUFA content in the human diet has been recognised in the industry literature; see for instance the article entitled" Omega 3 Enriched Pork" by Peter R.C- Howe Department of Biomedical Science, University of Wollongong, NSW, Australia For an enriched omega 3 product to be regarded as a useful dietary source it should be able to increase the level of omega 3 fatty acids present in the circulation when consumed in reasonable quantities, Although increases have been reported for consumption of omega 3 eggs there has been no similar demonstration of potential benefit to humans with other alternative dietary sources of marine omega 3 fatty acids apart from refined fish oil supplements.

In recognition of the benefits of long chain omega 3 PUFA, the food industry turned its attention to identifying an economic and plentiful source of these acids. Fish oil was considered an obvious source of these fatty acids and experimentation was conducted to determine the levels which could be used in pork recognising that levels above a desirable maximum, could result in taint of the pork.

An industry objective has therefore been to determine the extent of omega 3 PUFA enrichment of animal produce that could be achieved using fish meal without the problems of taint. Experimentation found that only very low percentages 0.8- 1.4% of fish oil could be used in the diet and it was found 3 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 19/55 00 ;Z preferable that the use should be terminated or reduced to the lowest levels before slaughter. The experiments demonstrated that increasing fish oil intake increased the percentage of long chain omega 3 Cl PUFA and therefore the nutritional qualities of the produce. It was also found that where diets contained 3% fish oil up to slaughter, the organoleptic quality of the pork was affected. The industry then turned its attention to the use of fish meal as a source of long chain omega 3 PI.IFA for the enrichment of pork but taint of the physical and sensory qualities of the pork remained a problem. The industry currently Cl o recommends that the fish meal content of pig rations not exceed 5% of the total diet and only where the 00 fish meal is withdrawn 5 7 weeks before slaughter. Where the pigs are fed fish meal up to slaughter it O is recommended that the level of fish meal in the diet not exceed The conventional source of fish Cl meal is pelagic fish, which when rendered into meal, normally has an oil content of 9%.

The applicant has for some time in order to meet the challenge of elimination of taint been involved in experimentation to determine fish meal formulae for feeding to animals depending upon the stage of development of the animal to which the food blend is fed to optimise the retention of omega 3 long chain PUFA but without taint cnused by the fish meal through off flavours and rancidity of the produce.

Although experts in the field have postulated as to possible ways to increase the use offish meal without taint of the produce, no one to date has succeeded in increasing the fishmeal level above 3 5% of the total diet without risk of taint of the produce. Some in the food industry advise against feeding fish meal at any time during the 14 day period before slaughter to eliminate the possibility of taint.

The August 1998 issue of the organ of the Fishmeal Information Network commissioned an independent study by a pig nutritionist to review the available data on use of fish meal in feeding pigs. The study found that fish meal, provided that it does not exceed 10% oil content, can be fed at up to 7.5% of the diet without presenting any problems of taint in the end product. The findings do however recognise that for 100% security against taint, percentage levels of fish meal in the diet should be set below Thus it is generally recognised in the industry that it is undesirable to increase the percentage of fishmeal beyond 3% 5% percent to be sure of elimination of taint.

INVENTION

4 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 20/55 00 0 0 S The present invention seeks to ameliorate or eliminate the aforesaid problems of the prior art relating to taint in produce by providing feed formulae which optimise the level of use of fishmeal in the diet of an Cl animal, above percentages previously achieved and without the problem of taint through off flavours and rancidity. According to one embodiment of the invention, fish meal from cannery scrap of oily fish S is used which produce a meal with 11% 13% oil containing approximately one third of omega 3 1- fatty acids.

Cl 0 00 In its broadest form the present invention comprises: an animal feed supplement utilising fish meal as a o source of omega 3 long chain fatty acids for inclusion in the diet of said animal; wherein, the fatty acids C1 are increased to a level which maximises the nutritional value of the produce from said animal due to increased level of long chain omega 3 fatty acids but without taint of said produce.

In another form the present invention comprises: a fishmeal food supplement for feeding to animals wherein the content of omega 3 long chain fatty acids in animal produce such as pork, eggs poultry meat, beef and farmed fish is maximised to a level which is nutritionally optimal but without taint of the produce; wherein the percentage of fishmeal food supplement is greater than 5% of the total dietary intake of the animal.

The percentage of supplement in the total diet of the animal is greater than 5% and preferably 15% to produce the long chain fatty acids Decosahexaenoic Acid (DFIA), Docosapentaenoic acid (DPA) and Eicosapentaenoic acid (EPA).

In another broad form the present invention comprises food supplement for feeding to animals in which the level of long chain omega 3 is increased to increase the nutritional quality of the animal produce and without taint of the produce; wherein the supplement includes a blend of fish meal, an oil based premix, a dry mix premix and water based green tea extract.

Preferably, the supplement constituents are blended according to the following proportions; Kg COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 21/55 Fish Meal Oil based premix Dry mix Premix Water based green tea 983.00 98.300 10.00 1.000 5.00 0.500 2.00 0.200 1000.00 100.000 1000.00 100.000 Preferably the oil based premix of the supplement comprises a blend of the following ingredients; Ethoxyquin vitamin grade Aniseed China Star Thyme white oil Benzoic Acid Crude Canolh oil Carrier.

Crude palm oil Rosemary oil Peppermint oil Phosphoric Acid 81% According to one embodiment, the constituents of the oil based premix per tonne of a food base are blended in the following proportions: Fthoxyquin vitamin grade Crude palm oil Aniseed China Star Rosemary oil Thyme white oil Peppermint oil Benzoic Acid Phosphoric Acid 81% Crude Canola oil Carrier.

Kg 0.400 4.00 1.500 15.00 0.067 0.67 0.100 1.00 0.100 1.00 0.150 1.50 0.005. 0.05 0.250 2.50 7.428 74.28 10.0 100.00 Preferably, the dry mix premix comprises a blend of the following ingredients; Vitamin E 50 Citric Acid Calcium Propionate Vitamin C Propy) Gallate Wheat Meal Carrier COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 22/55 Preferably, the dry mix premix comprises the following proportion of constituents; Vitamin E 50 Vitamin C Citric Acid Propyl Gallate Calcium Propionate Wheat Meal Carrier Kg 0.800 0.500 0.500 0.100 0.500 2.600 16.00 10.00 10.00 2.00 10.00 52.00 5.000 100.00 According to a preferred embodiment, the Vitamin C and Citric Acid are scavenger antioxidants. In another broad form the present invention comprises a food supplement for feeding to animals to maximise the level of long chain omega 3 fatty acids in the animal produce and without taint of the produce; wherein the supplement comprises a blend of fish meal, an oil based premix and a drymix premix blended in the following proportions: Fish Meal Oil based premix Dry mix Premix Kg 985.00 98.500 10.00 1.000 5.00 0.500 1000.00 100.000 In another form the present invention comprises a food supplement for feeding to animals to maximise the level of long chain omega 3 fatty acids in the animal produce and without taint of the produce; wherein the supplement comprises tuna meal, an oil based premix, a dry mix premix, water based green tea extract wherein the supplement includes the following constituents; Fish Meal Crude palm oil Rosemary oil Peppermint oil Ethoxyquin vitamin grade Aniseed China Star Thyme white oil Benzoic Acid COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/OG/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 23/55 Phosphoric Acid 81% Vitamin E 50 Citric Acid Calcium Propionate Crude Canola. oil Carrier.

Vitamin C Propyl Gallate Wheat Meal Carrier In another broad form the present invention comprises a food supplement for feeding to animals to maximise the level of omega 3 fatty acids in the animal produce and without taint of the produce; wherein the supplement comprises fish meal, an oil based premix, a dry mix premix and water based green tea wherein the fish meal, dry mix premix and oil based premix collectively include the following constituents; Tuna Meal Crude palm oil Thyme white oil Benzoic Acid Crude Canola oil Carrier.

Vitamin C Propyl Gallate Ethoxyquin vitamin grade Rosemary oil Peppermint oil Phosphoric Acid 81% Vitamin E Citric Acid In another broad form the present invention comprises a food supplement for feeding to animals to maximise the level of omega 3 fatty acids in the animal produce and without taint of the produce; wherein the supplement comprises fish meal, an oil based premix, and a water based green tea extract and a dry mix premix including the following relative proportions of constituents; Tuna Meal (containing 11% 13% oil) 98.500 Ethoxyquin vitamin grade 0.040 Crude palm oil 0.150 Rosemary oil 0.010 Thyme white oil 0.010 Peppermint oil 0.015 Benzoic Acid 0.001 Phosphoric Acid 81% 0.025 Crude Canola oil Carrier. 0.743 Vitamin E 50 0.080 Vitamin C 0.050 Citric Acid 0.050 Propyl Gallate 0.010 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2009 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 24/55 According to a preferred embodiment, the supplement further comprises the following constituent percentage proportions; Vanillic Crystals Aniseed China Star Calcium Propionate Wheat Meal 0.013 0.007 0.050 0.260 In another form the present invention comprises a food supplement for feeding to animals to maximise the level of long chain omega 3 fatty acids in the animal produce and without taint of the produce; wherein the supplement comprises fish meal, an oil based premix, a dry mix premix, and water based green tea extract blended in the following proportions per tonne of food base: Tuna Meal Ethoxyquin vitamin grade Crude palm oil Aniseed China Star Rosemary oil Thyme white oil Peppermint oil Benzoic Acid Phosphoric Acid 81% Crude Canola oil Carrier.

Vitamin E 50 Vitamin C Citric Acid Propyl Gallate Calcium Propionate Wheat Meal Carrier Kg 985.000 98.500 0.400 0.040 1.500 0.150 0.067 0.007 0.100 0.010 0.100 0.010 0.150 0.015 0.005 0.001 0.250 0.025 7.428 0.743 0.800 0.080 0.500 0.050 0.500 0.050 0.100 0.010 0.500 0.050 2.600 0.260 1000.000 100.000 The fish meal may be selected from Tuna, Mackerel, Sardine, Pilchard or Anchovies but it will be appreciated that marine fish oil may be derived from other species.

9 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 25/55 00 0 0 ;Z According to the method aspect of the present invention there is provided a method of feeding animals using a fish meal based food supplement with 11% 13% fish oil wherein the supplement comprises at C'l least 5% 20% of the animals' total diet wherein the method comprises the following steps: a) selecting constituents for a supplement including fish meal, an oil based premix, a dry mix premix en3 and a water based green tea extract 00 b) preparing the dry mix and the oil based premix; C] c) blending the constituents in predetermined proportions of the supplement; d) selecting additives for the supplement appropriate to the stage of development of the animal and blending the additives with the fish meal, oil based premix, dry mix premix and water based green tea extract; e) feeding an animal with the supplement blended according to the stage of development of the animals selected for feeding so that thi, food produce from said animals has an optimum level of long chain omega three fatty acids yet is free from taint and wherein the supplement may comprise between 5% of the total diet of the animal.

According to another form of the method aspect of the present invention there is provided a method of feeding animals using a fish meal based supplement at a level within the range of 10% 20% of the animal's total diet, wherein the supplement comprises the following active ingredients: Tuna Meal Ethoxyquin vitamin grade Crude palm oil Rosemary oil Thyme white oil Peppermint oil Benzoic Acid Phosphoric Acid 81% Crude Canola oil Carrier.

VitaminE Vitamin C Citric Acid COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/208 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 26/55 00 0 0 ci Propyl Gallate wherein the method comprises the following steps: a) selecting constituents for a supplement including fish meal, an oil based premix, a dry mix premix and a water based green tea extract 00 b) preparing the dry mix and the oil based premix; 0 c) blending the constituents in predetermined proportions of the supplement; d) selecting additives for the supplement appropriate to the stage of development of the animal and blending the additives with the fish meal, oil based premix, dry mix premix and water based green tea extract e) feeding an animal with the supplement blended according to the stage of development of the animals selected for feeding so that the food produce from said animals has an optimum level of long chain omega three fatty acids yet is free from taint and wherein the supplement comprises between 5% of the total diet of the animal.

According to a preferred embodiment the method comprises the further step prior to or after blending of the dry mix and fish meal of: a) preparing the ingredients of said oil emulsion according to the following mixing sequence; Crude Canola Oil Crude Palm Oil Phosphoric Acid 8 1% Benzoic Acid Ethoxyquin Vitamin Grade Aniseed China Star Rosemary Oil Thyme White Oil Peppermint Oil 11 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:1e +61-2-95522894 WALSH AND ASSOCIATES PAGE 27/55 b) high speed mixing of said ingredients to create a homogeneous stable emulsion.

According to the preferred embodiment, the method includes the following step prior to steps a) and b) last mentioned above of preparing the ingredients according to the following steps: i) heating to above 50 degrees C to liquefy the Crude palm oil; ii) heating to above 22 degrees C to liquefy the Aniseed china star iii) heating to above 20 degrees C to liquefy the Crude Canola Oil.

According to a preferred embodiment the method comprises the further step prior to or after blending of the oil emulsion and fish meal of preparing the dry mix by combining the following ingredient sequence: Wheat Meal Vitamin C Propyl GalIate Green Tea Powder Vitamin E Citric- Acid Calcium Propionate Vanil Iic Crystals According to one embodiment of the method aspect, the Oil Emulsion may be applied by fine spray to the Fish Tuna meal; the Aqueous Extraction to be applied by fine spray to the Tuna meal; the dry mix to be added to the Tuna meld followed by mixing the whole to produce a homogeneous blend.

Nutritional Premixes supplying the Vitamin, Mineral requirements of particular species and stage of life cycle/development may be included in the supplement to satisfy the nutritional completeness of the appropriate supplement According to the invention, the feed supplement provides a supply of long chain fatty acids such as DHA, DPA and EPA in contrast to the short chain fatty acids which are obtained from certain vegetable sources. The long chain omega 3 fatty acids will be absorbed directly into the animal body in their 12 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 IA 1Q LCI jjnrrnnflA o WA

L

bH AND ASSOCIATES PAGE 28/55 00 0 0 (-x existing biological form exertin.g beneficial physiological change and direct deposition in the blood stream, body cells and fat depots unlike the Short Chain Fatty Acids which require conversion by the (N animal to long chain fatty acids which is inefficient and poor yielding.

The present invention will now be described according to preferred but non limiting embodiments and with reference to various examples wherein; 00 Figure 1 shows, a schematic layout of a typical process for the preparation of a feed supplement according to one embodiment of the invention.

(N Annexure 1 shows a variety of supplement formulae according to various embodiments of the invention and adapted for feeding at various stages of development of an animal.

The supplement blends according to the examples to be described provide a consistent high source of omega 3 long chain fatty acids, especially DHA in animal diets to thereby increase the intake of omega 3 long chain fatty acids in the human diet. The supplement blends have been found to eliminate taint of the resultant produce and to enhance the flavour of the produce. Conventional wisdom prior to the invention required the implementation of at least a 14 day fishmeal exclusion prior to slaughter to avoid fish taint. This 14 day exclusion period presents practical difficulties on farms where the producer is forced by farm circumstances to operate with a single stage finishing diet. In some cases it could lead to the withdrawal of fishmeal in the diet at a much earlier stage with possible adverse implications for livestock health. There is concern that the 14 day exclusion could be increased for many weeks and possibly up to 12 weeks. Experts currently believe that when feeding fishmeal up to 5% of the dietary intake of the animal there is a comfortable safety margin for eliminating off flavours in the animal produce, provided that as an added safeguard fishmeal is excluded from the diet two months before slaughter.

The present invention challenges convention wisdom and overcomes the problems of prior art fishmeal feed formulae by providing a range of fishmeal feed supplements which may be significantly above the currently recognised upper percentage limits for elimination of taint but which do not result in taint of the produce.

13 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/0612008 23:18 -cF~1--3--~FF?300n Ilnl ~TI I 21/0/2008 23:1. WALbsM NU ASULUiAIES PAGE 29/55 00 (1 C' Taint in foodstuffs is normally determined by a Sensory Panel of experts who are trained in determining whether a particular foodstuff has certain taste characteristics. Thus one expert may have a proven sensitivity to sweetness, in cheese and another in meats. Other experts can determine whether animal produce is tainted with a fishy taste after feeding with fish meal. Trials were conducted to determine whether animal produce from animals fed with the feed supplements and associated formulae according to the present invention were tainted Although prior to the invention, experts believed that fishmeal supplements greater than 5% of the total diet of the animal would lead to fishy taint, the Sensory Panel 00 O evaluating the Supplements and formulae according to the invention marketed under the trade mark C, name Porcomega fed to pigs found that supplements greater than 5% of the total diet of the animal did not result in taint of the produce.

Tests on the effects on animal produce of fish meal supplements according to the invention and greater than 5% of the total diet of the animal were carried out in the Commonwealth Scientific and Industrial Research Organisation Department of Human Food Nutrition in Adelaide, Australia. The panel found that the produce tested did not suffer from fishy taint.

Preferably, the percentage range of fishmeal with fish oil content between 11% 13 in the diet of an animal in which omega 3 fatty acid enrichment is required is 5% 20%. Due to the blends of the supplements used, the fishmeal may be fed up to slaughter in these percentages. Figure I shows a schematic layout of a typical process for the preparation of feed supplements according to the method aspect of the present invention.

According to the method aspect of the invention, quality control is most important for conformity of the finished supplement and its raw materials. As a matter of routine practice, the constituent ingredients should be checked for physical conformity and condition. Preferably, chemical analyses are carried out and with checks for any infestations from insects etc. The ingredients must be free from rancid, musty, stale or any other objectionable odours. Therefore, thorough physical examination is important.

14 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/08/2008 23:19 +61-2-95522994 WALSH AND ASSOCIATES PAGE 30/55 00 Prior to the start of each production run and after any extended downtime, the process line is evaluated to verify that the line is in satisfiactory conldition and that allI controls are calibrated and functioning. This is described as calibration step 1.02.

Step 1. 1 involves the preparation of the dry mix wherein hulk ingredients 1. 1] are selected according to M the particular formula, followed by calibration step 1.12 involving weighing out bulk ingredients. The C~1 ingredients are then transferred for mixing. Step 1.3 is Preparation of the oil based premix. Preparation Cl of the oil based premix involves the following steps. The raw materials are selected and weighed 1.31 00 O according to the selected formula. These materials are mixed 1 .32 by stirring with preweighed preheated degrees C oil carrier in a steam jacketed mixing vessel. The mix is then held for quality control step 1.33 followed by calibration step 1-35 wherein the mix is metered into the dry mixer according to formula.

Step 1.4 is the preparation of the Dry Mix Premix. Step 1.41 is the selection and weighing of the ingredients according to formula. In step 1.42 the ingredients are mixed together followed, by quality control step 1.43 and calibration step 1.44 wherein the mix is metered into the bulk dry mixer according to formula. The next phase step 1-5 involves the following procedure. Step 1.51 is the selection and weighing of ingredients- according to formula. Int step 1.51 ingredients are added to preweighed water heated to 100 degrees C in thes:teamn jacketed reaction vessel followed by mixing step 1.53. In step 1.54 a preweighed Green Tea ingredient retained in a cloth bag is infujsed into the mix. In step 1.55 thle green tea is extracted for 30 minutes and the spent Green Tea is discarded. Step 1 .56 involves metered mixing according to formula into the bulk dry mixer. According to steps 1. 15 to 1. 19 the mixture is mixed for minutes until it is homogenous following which the mixture is discharged and sieved through a 2mm screen wherein the tailings are discarded. The mixture is then subject to quality control According to steps 1.6 to 1.63 the mixture is released for weighing, checking, packing and despatch.

Trials in human volunteers were undertaken to test the hypothesis that regular consumption of pork enriched with long chain omega 3 fatty acids will lead to increased levels of these fatty acids in the circulation and resultant improvement in cardiovascular health.

COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2009 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 31/55 00 0 0

(N

The trial objective was to conduct a preliminary dietary intervention study with pork from pigs fed with 1 supplements according to the present invention to determine of there would be any increase in the levels Ci of EPA, DPA or DHA present in the plasma fatty acid pool or in erythrocyte RBC membrane phospholipids in human volunteers eating lean cuts of pork over a 4 week period. The tests endeavoured S to determine whether the increase in omega 3 fatty acid supplementation would influence the two health 7. parameters that are reportedly most responsive to omega 3 fatty acid supplementation namely reduction O of plasma triglycerides and platelet thromboxane production.

00 O A study was conducted to determine how much omega 3 enrichment could be achieved using fish meal.

OC Nine week old pigs were fed a ration containing 20% of a high grade fishmeal supplement formula according to the present invention. EPA, DPA and DHA accounted for 0.5% and respectively of total fatty acids in the diet. There were negligible amounts in a control diet. Groups of three pigs were fed the control diet for 7 or 11 weeks or the fish meal diet for 6 to 10 weeks before slaughter. Growth and feed conversion rates were similar in control and fish meal fed pigs. In the latter, omega 3 consumption averaged 15g/pig/day which resulted in striking increases in the plasma levels of DHA and particularly EPA. In the week before slaughter the high circulating levels declined but there was significant retention of long chain omega 3 polyunsaturated fatty acids including DPA in lean portions sampled from various cuts of fresh pork which increased with the duration of feeding. Graph I below depicts relative proportions of long chain fatty acids in selected cuts of meat from pigs fed a control diet of the fishmeal supplement according to the invention for 10 weeks. Graph 2 shows omega 3 concentrations in selected cut; of meat from pigs fed a control diet of the fish meal supplement according to the present invention. The proportion of long chain omega 3 polyunsaturated fatty acids in lean loin chops 4.3% fat rose from 1.1% in controls to 6% in the fish meal fed pigs representing a yield of 250mg/100g of fresh meat. In forequarter chops the proportion was less 4. 1% but the fat content was higher 8.7% Hence the omega 3 yield was even greater: 360mg/100g. Furthermore the surrounding layer of fat contained five times as much long chain omega 3 fatty acids. The study indicates that the omega 3 yield of pork products fed with a supplement according to the invention will depend upon their overall fat content.

16 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +GI-2-95522894 WALSH AND ASSOCIATES PAGE 32/55 ~[46i-dIje-oG -fl Lifete AOG

IL

SAO.

L 0 avrg .,-gi pr~aoa u e-ih~I Lifetime avrge daily gain GRAPH I Ghnge In Llvenfighl I 2.3 3 a Brth 21d 5 d 112 d 147 d Age 1dj GRAPH 2 Change in Livewveight over 'ime.

I"a(, COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:1e +61-2-95522894 WALSH AND ASSOCIATES PAGE 33/55 Twenty seven volunteers participants and their partners completed the sensory evaluation of cooked pork. The results appear in Table 1 below and demonstrate no significant preference for either the n-3 enriched pork or the control pork. Most importantly the volunteers did not detect any preference for one pork over the other indicating that there was no detectable taint in the pork.

TABLE 1 Sensory evaluation of pork.

Prefer Porcomega Prefer normal pork No preference Loin taste 7 12 8 smell 5 2 mouthfeel 11 8 8 Forequarter taste 6 10 11 smell 6 6 smelt 6 6 mouthfeel

OVERALL--

OVERALL

7 9

II

7.0 7.8 12--- 7.0 7.8 12.2 Detailed studies have been carried out on the carcasses of pigs fed with the supplement formula according to a regime commensurate with the stage of development of the pig. Results of the analyses of the carcasses vary according to the site. Tables 1-5 below set out examples of the formulae of the food supplements according to various embodiments of the invention. It was found that there were no significant dietary effects of the inclusion of the supplements according to the invention, fishmeal or fish oil on growth rate and slaughter characteristics of the pigs in this study. The meat from all diets were found acceptable to consumers. None of the produce from Diets 1-5 were considered unacceptable. The determination of fatty acid profiles in meat samples was carried out by Professor Len Storlein of the Biological Sciences Department of the University of Wollongong New South Wales The dietary 17 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06 /2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 34/55 supplements are tailored to suit the stage of development of the pig. Of the Diets identified below, Diet is the least preferred in favour of diets 1-4.

TABLE 2 DIETS

CONTROL

CONTROL 3% FISH OIL

SEAPEP

SUPPLEMENT ACCORDING TO THE INVENTION (Porcomega) SUPPLEMENT ACCORDING TO THE INVENTION Porcomega) 3% FISH OIL The following examples show that the omega 3 long chain fatty acid content of pork is significantly increased in pigs fed supplements in accordance with the present invention. The examples tabulate the fatty acid profile of phospholipid and Triglyceride from specified sites of male and female pigs for each of the diets identified in Table 2.

EXAMPLE 1 Fatty Acid Profile DHA, EPA, DPA of Phospholipid from Three Sites Loin, Leg, Forquarter) in 5 MALE PTGS LOIN as of Fat)

DIET

1. Control 2. Control 3% Fish Oil 3. 15%Seapep 4. 15% Porcomega 15% Porcomega 3% Fish Oil 1.7177 4.5484 5.5875 5.875 9.4645 DHA EPA 0.7430 1.6755 4.7766 1.2947 2.9664 1.6813 4.2736 1.8344 3.0052 0.7113

DPA

5.035 11.679 11.037 12.941 13.181 Fotal n-3 7.044 2.807 3.088 2.600 2.219 n6/n3 LEG (as of Fat)

DIET

DHA EPA DPA Total n-3 n6 n3 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 35/55 Control Control 3% Fish Oil Seapep Porcomega Porcomega 3% Fish Oil 1.2720 6.6598 6.2464 8.5052 7.5433 0.5703 4.8659 1.9129 3.0288 5.6210 1.0655 1.4591 2.0729 1.0103 0.0000 4.2150 13.309 11.376 12.544 13.164 9.933 1,943 2.994 2.165 2.204 LOIN as of Fat)

DIET

DHA EPA DPA Total n-3 n6 n3 Control Control 3% Fish Oil Seapep Porcomega Porcomega 3% Fish Oil 1.1029 6.3329 6.7154 7.5528 8.3222 0.6498 0.8971 3-655 10.430 5.6516 1.5256 14.293 2.160 0.5542 1,6361 10.189 3.234 4.6347 0.1816 13.381 2.349 5.0940 0-0000 13.332 1.889 EXAMPLE 2 Fatty Acid Profile (DHA, EPA, DPA of Phospholipid from Three Sites Loin, Leg, Forquarter) in 5 FEMALE PTGS LOIN as of LOIN as of Fat

DIET

DHA EPA DPA Total n-3 n6 n3 Control Control 3% Fish Oil Seapep Porcomega Porcomega 3% Fish Oil 2.7958 1.5085 1.5652 6.941 5.439 6.4429 6.2143 1.9654 15.564 1.839 6.1195 3.9108 1.9291 12.545 2.626 6.3399 6.9938 2.3628 16.132 2,018 7.6822 7.3558 1.8541 17.537 1.673 EXAMPLE 3 Fatty Acid Profile DA, EPA, DPA of Triglyceride from Three Sites COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/208 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 36/55 Loin, Leg, Forquarter) in 5 FEMALE PIGS LOIN as of Fat) DIET DHA EPA DPA 1. Control 0.2110 0.0519 0.1273 0.585 2. Control 3% Fish Oil 0.8181 0.2441 0.3250 1.684 3- 15% Seapep 0.6097 0.0000 0.0000 0.610 4. 1 5%Porcomega 0.8410 0.3872 0.0000 1.228 15% Porcomega 3% Fish Oil 0.7160 0.2839 1.1113 2.111 Total n-3 20.546 1.844 3.061 3.070 0.892 n6/n3 EXAMPLE 4 Fatty Acid Profile DHA, EPA, DPA of Triglyceride from Three Sites (Loin, Leg, Forquarter) in 5 MALE PIGS LOIN (as of Fat) DIET DHA EPA DPA Total n-3 n6 /n3 1. Control 0.3949 0.0000 0.1557 0.740 11.041 2. Control 3% Fish Oil 4.2856 1.3361 0.6500 6.673 1.801 3. 15% Seapep 1.3639 0.2781 0.1501 1.792 3.222 4. 15% Porcomega 1.5530 0.3823 0.1366 2.072 2.720 15% Porcomega 3% Fish Oil 1,7744 0.5098 0.2049 2.608 1.509 A trial using 25 large white female pigs and the above Diets was commenced on 12 December 1996 with a mean lightweight 21.5 kg running through slaughter on 12 March 1997. At slaughter the pigs were weighed and values recorded. Sub samples from each sex and dietary treatment were taken for taste and taint evaluations. There were no signs of significant weight gain. There were no apparent differences in weight gain of 1he male pigs due to dietary treatment. A similar result occurred with females and there were no significant differences due to type of fishmeal or oil added to the diet.

COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 37/55 The following table 3 shows carcass and performance data for female pigs. Combined (male and female statistical analysis of Figure 2 and table 2 showed a significant difference P 0.0001 in dressing percentage between the male and female pigs. The effect of sex approached traditional significance P 0.06 for ADG. There were no significant effects of diet (type of fishmeal or oil on any parameter nor any significant interactions of sex and diet.

TABLE 2 Diet 1 5 2 5 3 5 4 5 5 No Slaughter

LWT

100.4 103.8 102.1 100.1 103.7 Wt (kg)

DWT

69.4 70.7 70.7 69.3 73.0 DR% P 2(mm) Gain (kg) ADG (g/d) 69.1 68.1 69.3 69.2 70.4 18.8 18.2 15.2 18.6 17.4 79.0 81:9 81.2 79.3 81.3 Average daily gain calculated over an 89 day p:riod.

Meat from each diet were evaluated for taste and taint. Meat was cooked in boiling water for minutes. Small pieces of 1m3 from each treatment were evaluated for pork taste with reference to a scale of 0-10 in which 10 is extremely tasty and for taint on a scale of 1-10 in which 10 is extremely tainted The results of the tests are set out in table 3 below.

TABLE 3 The effect of diet on the taste and taint of male and female pig meat.

Diet Subjects Taste SE* Taint 10) SE Diet Subjects I Taste(0-10) SE* Taint(O-10)

SE

1 10 6.6 2 10 5.8 3 10 5.7 4 10 5.1 10 4.2 Standard- rror f th- mn--- Standard error of rhc mean.

;5 0 5 0 0 0.49 0.49 0.49 0.49 0.51 1.15 1.95 2.45 3.30 4.35 0.63 0.63 0.63 0.63 0.64 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/O/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 38/55 00

O

O

ci There were no significant effects of sex (of pig) on either taste or taint in this test. The results indicate no deleterious effects on either growth rate, carcass yield or carcass characteristics. Analysis indicates C] that the feed supplements according to the invention enhance the omega 3 long chain Fatty acids content of the meat. The feed supplements may be used with or without I- withdrawal periods prior to slaughter.

C] Clinical trials show a beneficial thromboxane result having the desired consequential physiological

O

C effects.

00 0 O A good measure of ration efficiency is the amount of feed required by a growing animal for it to gain a unit of weight. Feed represents 60% 70% of the running costs of a piggery. It has been estimated that a 0.1 unit change in feed efficiency is worth about AU$40/sow/year in terms of feed. During the lactation phase, PFM fed sows consumed approximately 84kg of feed each for a net gain of 10 kg. Based on this their feed gain ratio was 8:4:1. Over the same period' the feed gain ratio for the pigs fed with the supplement according to the invention was 3:7:1 74kg feed for 20kg gain The Graph 3 below shows feed gain ratios and it can be seen that gain for grower pigs was best for those fed supplement according to the invention followed by PFM pigs and control pigs A taste panel found that there was a preference for pigs fed with the supplement as the PFM pigs suffered from fishy taint which was detected by all members of the taste panel, GRAPH 3 '.r Feed:gain ratio's (A low ftd:gain rllon is best, 3 is btllr ths 3.6) COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 39/55 00 0 ;1 Annexure 1 sets out a range of supplement formulae according to embodiments of the invention which S relate to the stage of growth of an animal which is fed the supplement.

¢cn C It will be recognised by those skilled in the art that the compositions and supplement formula disclosed Cl are examples only and that these or variations thereof may be fed to other animals such as but not 00 o limited to beef, cattle, sheep and poultry to achieve the objects of the invention and as such are therefore within the spirit and scope of the invention broadly described herein. For example, the consistency of the premixes before addition may be varied. The flavour of the system may be altered for instance using Fenugreek.

23 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 40/55 ANNEXURE1 EMBODIMENTS OF SUPPLEMENT 1. FORMULA PER TONNE PORCOMEGA* PIGLET STARTER EW 001 kg kg PORCOMEGA* BASE- SP VITAMIN PREMIX LW 001 MINERAL PREMIX PM 001 AMINO ACID PREMIX PAA 001 CHOLINE CHLORIDE 60% 970.80 97.080 2.50 0.250 10.00 1.000 10.00 1.000 6.70 0.670 1000.00 100.000 USAGE: 150 KG PER TONNE OF PIGLET STARTER EW DIET.

1. PIG STARTER EW VITAMIN PREMIX PV 001

COMPOSITION

COMPOSITION

INGREDIENT

POTENCY/GM

VITAMIN A-500 500.000 IU VITAMIN D3-500 500,000 IU VITAMIN E-50% 500 IU VITAMIN K3 1000mg THIAMINE HYDROCHLORIDE USP 892 mg RIBOFLAVIN 95% F.G. 950 mg PYRIDOXINE USP 823 mg VITAMIN B12-SUPPLEMENT 1% 10 mg NIACIN USP 990mg CALCIUM (d-PANTOTIHENATE USP 920 nmg FOLIC ACID USP 920 mg D-BIOTIN SUPPLEMENT 1% 10 mg GREEN TEA POWDER Anti Oxidant 1000 Mg VANILLIC CRYSTALS 1000 g 24

FORMULA

53.60 gm 13.40 gm 52,80 gm 4.02 gm 4.51 gm 11.28 gm 6.51 gm 5.36 gm 54.14 gm 34.96 gm 4.37 gm 21.44 gm 120.00 gm 53.60 gm

ACTIVE

/Kg PREMIX 26,800,000 1U 6,700,000 IU 26,400 IU 4.02 gm 4.02 gm 10.72 gm 5.36 gm 53.60 mg 53.60 gm 3 2 1 6 gm 4.02 gm 2 14 4 0 mg 120.00 gm 53.60 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/6/208 23:16 +61-2-95522894 WALSH AND ASSOCIATES PAGE 41/55 WHEAT MEAL CARRIER 560.01 gm 1000.00 gm USAGE: 2.5 Kg per Tonne Porcomega* Pig Starter EW Supplement 001.

PIG STARTER EW. PORCOMEGA* AND COMPLETE FEED VITAMIN

ADDITION

COMPOSITION ACTIVE

ACTIVE

INGREDIENT /Tonne PORCOMEGA* /Kg COMPLETE

FEED

V o- VITAMIN A-500 67.000,000 I U 10,000 IU VITAMIN D3-500 16,750,000 LU 2.500 IU VITAMIN E-50% 466.000 IU 69.500 1U VITAMIN K3 10.05 gmrn 1.50 mg THIAMINE HYDROCHLORIDE USP 10.05 gm 1.50 mg RIBOFLAVIN 95% F.G. 26.80 gm 4.00 mg PYRIDOXINE USP 13.40 gm 2.00 mg VITAMIN B1I2-SUPPLEMENT 1% 134.00 mg 2 0.0 mg FOLIC ACID USP 10.05 gm 1.50 mg D-BIOTIN SUPPLEMENT 1% 5 36 .00 mg 80.00 meg CHOLINE CHLORIDE 4020.00 gm 600.00 mg GREEN TEA POWDER 300.00 gm 44.77 mg VANILTTIC CRYSTALS 134.00 gm 20.00 mg PIG STARTER EW MINERAI, PREMIX PM 001

COMPOSITION

INGREDIENT

POTENCY/GM

FERROUS SULPHATE 310.0 mg S04.H20] ZINC SULPHATE MANGANESE

SULPHATE

[Mn S04.H20] POTASSIUM IODIDE

(STAB)

COBALT

CARBONATE

COPPER

SULPHATE

360.0 mg 280.0 mg 68.8 mg 460.0 mg 250.0 mg

FORMULA

2 16 .125 gm 186.110 gm 95.715 gm 0.488 gm 0.728 gm 33 5.000 gm

ACTIVE

/Kg PREMIX 67.000 gm 6 7 .000gm 26 .800 gm 0.335 gm 0.335 gm 83 .750 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/062008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 42/55 USOIUM SELENITE 400.0 mg 0.50 4 gm LIMESTONE CARRIER 1000.0 mg 165.330 gm 1000.000 gm USAGE: 10 Kg per Tonne Porcomega* Pig Starter EW Supplement 001.

0.201 gm 165.330 gm PIG STARTER EW PORCOMEGA* AND COMPLETE FEED MINERAL ADDITION

COMPOSITION

INGREDIENT

FERRQUS SULPHATE-Fe [Fe 504.1120] ZINC SULPHATE-Zn MANGANESE SULPHATE-Mn [Mn 504.1-120] POTASSIUM IODIDE (STAB)-I COBALT CARBONATE-Co COPPER SULPHATE-Cu SODIUM SELENITE-Se

ACTIVE

/Tonne PORCOMEGA* 670.00 gm

ACTIVE

/Kg COMPLETE FEED 100.500 mg 670.00 gm 268.00 gm 3.35 gm 3.35 gm 837.50 gm 2.01 gmn 100.500 40.200 mg 0.502 mg 0.502 mg 125.625 mg 0.301 mg PIGLET STARTER EW AMINO ACID PREMIX PAA 001

COMPOSITION

INGREDIENT

1-LYSINE dl-METHIONINE

THREONINE

WHEAT MEAL CARRIER

FORMULA

6700.00 gm 1340.00 gm 1340.00 gm 620.00 gm 26 /Kg PREMIX 670.00 gm 134.00 gm 134.00 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 43/55 10000.00 gm USAGE: 10 KG PER TONNE OF PORCOMEGA* PIGLET STARTER EW 001.

AMINO ACID ADDITIONS /Tonne PORCOMEGA* /Kg COMPLETE FEED

I-LYSINE

d 1-METHTONINE

THREONINE

6700.00 gm 1340.00 gm 1340.00 gm 1000.00 mg 200.00 mg 200.00 mg 2. FORMULA PER TONNE PORCOMEGA* PIG WEANER 002 Kg Kg PORCOMEGA* BASE SP VITAMIN PREMIX PV 002 MINERAL PREMIX PM 002 AMINO ACID PREMIX PAA 002 CHOLINE CHLORIDE 60% 970.80 97.080 2.50 0.250 10.00 1.000 10.00 1.000 6.70 0.670 1000.00 100.000 USAGE: 150 KG PER TONNE, OF PIG WEANER DIET 002.

2, PIG WEANER VITAMIN PREMIX PV 002.

COMPOSITION'

INGREDIENT

POTENCY/GM

VITAMIN A-500 VITAMIN 3-500 VITAMIN E-50% VITAMIN K3 THIAMINE HYDROCHLORIDE U5P RJBOFLAVIN 95% F.G.

PYRIDOXINE UISP VITAMIN B12-SUPPLEMENT 1% NIACIN USP CALCIUM CI-PANTOTFIENATE

USP

FOLIC ACID USP D-BIOTIN SUPPLEMENT 1% 500.000 IU 500,000 IU 500 IU 1000 mg 892 mg 950 mg 823 mg 10 mg 990 mg 9 2 0 mg 920 mg 10mg

FORMULA

53.60 gm 13.40 gm 52.80 gm 4.02 gm 3.00 gm 9.87 gm 4.88 gm 4 .69 gm 40.61 gm 34.96 gm 4.37 gm 13.40 gm

ACTIVE

/Kg PREMIX 26.800,000 IU 6.700,000 IU 26,400 IU 4.02 gm 2.68 gm 9.38 gm 4.02 gm 46.90mg 40.20 gm 32.16gm 4.02 gm 134.00 mg COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:IG 21/0/208 2:19 +1-2-95522894 WALSH AND ASSOCIATES PG PAGE 44/55 GREEN TEA POWDER VANILLIC CRYSTALS WHEAT MEAL CARRIER 1000 Mg 120.00 gm 53.60 gmf 580.07 gmn 1 000.00 gmn 120.00 gm 53.60 gin USAGE: J.5 Kg per Tonne Porcomega 4 Pig Weaner Supplement 002- PIG WEAlER- FORCOMEGA. AND COMPLETE FEED VITAMIN ADDITION COMIPOSkTION ACTIVE ACTIVE TNGREDIN /Tonne PORCOMEGA* /Kg COMPL, VJTA~M~rI A-500 67,000,000 IU 10,000 LU VTTAMiI D3-500 16,750,000 IU 2.500 JU VITAMI1 E-50% 466.000 IU 69.5 TU VITAM~h K3 10.05 gm 1-50mg T141AMW NE I-IYDROCHLORI)E USP 6.70 gm 1.00 ing RIBOFLAVIN 95% F.G. 23.45 gmn 3.50 mg PYRLDO*FINE USP 10.05 gmn 1.50mg VITAMYf B12-SUPPLEMENT 1% 117.25mg 17.50 mng NJACINIITSP 100.50 gm 15.00 mg CAL.CIU4 c-PANTOTHENATE LJSP 67.00 gmn 10.00 Mg FOLIC A&IDUSP 10.05 gm 1.50 Mg D-1BJOT1{SUPPLEMENT 1% 335.00 mg 50.00 Mg CHLN-CHLORIDE 3350.00 gmn 500.00 mg GREEN tiEA POWDER 300.00 gm 44.77 mng VANILLI& CRYSTALS 134.00 gm 20.00 mg ETE FEED PIG WEAJNER MINERAL PREMIX PM 002 CON4POS[TION IN GREDIlENT

ACTIVE

POTENCY/GM FORMULA /Kg PREMIX FE RROUS SULPHATE [Fe S04.H20] ZINC SULPHIATE-Zn MANGANESE SULPI-IATE 3100 mg 360.0 mg 280.0 ing 216.125 gm 186.110 gm 95.7 15 gmn 67.000gm 67.000 gmn 26.800 gmn COMS ID No: ARCS-i 95426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 45/55 [Mn S04.11201 POTASSIUM IODIDE(STAB)-I 68.8 mg COBALT CARBONATE 460.0 mg COPPER SULPHATE 250.0 mg SODIUM SELENITE 400.0 mg LIMESTONE CARRIER 1000.0 mg 0.488 gm 0.728 gm 335 .000 gm 0.504 gm 165.330 gm 1000.000 gm 0.335 gm 0.335 gm 83.750 gm 0.201 gm 165.330 gm USAGE: 10 Kg per Tonne Porcomega* Pig Weaner Supplement 002.

PIG WEANER PORCOMEGA* AND COMPLETE FEED MINERAL ADDITION COMPOSITION ACTIVE

ACTIVE

INGREDIENT /Tonne PORCOMEGA /Kg COMPLETE FEED FERROUS SULPHATE-Fe 670.00 gm 100.500 mg [Fe S04.H20] ZINC SULPHATE-Zn MANGANESE SULPHATE-Mn [Mn S04.FI201 POTASSIUM IODIDE (STAB)-I COBALT CARBONATE-Co COPPER SULPHATE-Cu SODIUM SELENITE-Se 670.00 gm 268.00 gm 3.35 gm 3.35 gm 837.50 gm 2.01 gm 100.500 mg 40,200 mg 0.502 mg 0.502 mg 125.625 mg 0.301 mg PIGLET WEANER AMINO ACID PREMIX PAA 002

COMPOSITION

INGREDIENT

FORMULA

/Kg PREMIX I-LYSINE 6700.00 gm 29 670.00 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 46/55 dI-METHONINE

THREONINE

WHEAT MEAL CARRIER 1340.00 gm 1340.00 gm 620.00 gm 134.00 gm 134.00 gm 10000.00 gm USAGE: 10 KG PER TONNE OF PORCOMEGA* PIG WEANER SUPPLEMENT.

PTG WEANER AMINO ACID PREMIX PAA 002 AMINO ACID ADDITIONS

I-LYSINE

dl-METHONTNE

THREONINE

/Tonne PORCOMEGA* 6700.00 gm 1340.00 gm 1340.00 gm /Kg COMPLETE FEED 1000.00 mg 200.00 mg 200.00 mg 3. FORMULA PER TONNE PORCOMEGAS PIG BREEDER 003 PORCOMEGA* BASE SP VITAMIN PREMIX PV 003 MINERAL PREMIX PM 003 987.50 2.50 10.00 1000.00 98.750 0.250 1.000 100.000 USAGE: 150 KG PER TONNE OF PIG BREEDER DIET 003.

3. PIG BREEDER VITAMIN PREMIX PV 003

COMPOSITION

INGREDIENT

POTENCY/GM

VITAMIN A-500 500,000 IU VITAMIN K3-500 500,000 IU VITAMIN E-50% 500 IU VITAMIN K3 1000 mg THIAMINE HYDROCHLOR1DE USP 892 mg RIBOFLAVIN 95% E.G. 950 mg PYRIDOXINE USP 823 mg VITAMIN B 12-SUPPLEMENT 1% j0 mg NIACIN USP 990 mg CALCTUM d-PANTOTHENATE USP 920 mg FOLIC ACID USP 920 mg

FORMULA

53.60 gm 5.36 gm 134.00 gm 10.05 gm 4.51 gm 14.11 gm 4.88 gm 4.02 gm 54.14 gm 29.13 gm 2.91 gm

ACTIVE

/Kg PREMIX 26,800,000 JU 2.680,000 JU 67,000 IU 10.05 gm 4.02 gm 13.40 gm 4.02 gm 40.20mg 53.60gm 26.80 gm 2.68 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 47/55 D-BIOTIN SUPPLEMENT 1% GREEN TEA POWDER VANTLLIC CRYSTALS WHEAT MEAL CARRIER 10mg 1000 mg 53.60 gm 120.00 gm 13.40 gm 496.29 gm 1000.00 gin 536.00 mg 120.00 gm 13.40 gm USAGE: 2.5 Kg per Tonne Porcomega* Pig Breeder Supplement.

PIG BREEDER- PORCOMEGA* AND COMPLETE FEED VITAMIN ADDITION

COMPOSITION

INGREDIENT

VITAMTN A-500 VITAMIN D3-500 VITAMIN E-50% VITAMIN K3 THIAMINE HYDROCHLORIDE USP RIBOFLAVIN 95% E.G.

PYRIDOXINE USP VITAMIN B12-SUPPLEMENT 1% NIACIN USP CALCIUM d-PANTOTHENATE USP FOLIC ACID USP D-BIOTIN SUPPLEMENT 1% CHOLINE CHLORIDE GREEN TEA POWDER VANILLIC CRYSTALS

ACTIVE

/Tonne PORCOMEGA* 67,000,000 IU 6.700,000 IU 167,500 IU 10.05 gin 10.05 gm 3350 gm 10.05 gm 75.00 mg 134.00 gm 67.00 gm 6,70 gnn 134.00 mg 2010.00 gm 300.00 gm 33.50 gm

ACTIVE

/Kg COMPLETE FEED 10,000 IU 1,000 1U 25.0 IU 1.50 mg 1.50 mg 5.00 mg 1.50mg 15.00 mg 20.00 mg 10.00 mg 1.00 mg 200.00 mg 300.00 mg 44.77 mg 5.00 mg PIG BREEDER MINERAL PREMIX PM 003

COMPOSITION

COMPOSITION

INGREDIENT

FERROUS SULPHATE [Fe S04.-201

POTENCY/GM

310.0 mg 360.0 mg

FORMULA

216.125 gm 186.110 gm

ACTIVE

/Kg PREMIX 67.000 gm 67.000 gm ZINC SULPHATE COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 48/55 MANGANESE

SULPHATE

[Mn 804.1120] POTASSIUM IODIDE

(STAB)

COBALT CARBONATE COPPER SULPHATE SODIUM SELENITE LIMESTONE

CARRIER

2 80.0 mg 68.8 mg 460.0 mg 250.0 mg 400.0 mg 1000.0 mg 95 7 1 5 gm 0.488 gm 0.728 gm 13.400 gm 0.251 gm 48 7 .183 gm 26.800 gm 0.335 gm 0.335 gm 3.350 gm 0.101 gm 1000.000 gm USAGE: 10 Kg per Tonne Porcomega* Pig Breeder Supplement 003.

PIG BREEDER PORCOMEGA* AND COMPLETE FEED MINERAL ADDITION COMPOSITION ACTIVE

ACTIVE

INGREDIENT /Tonne PORCOMEGA* /Kg COMPLETE FEED FERROUS SULPHATE-Fe 670.00 gm 100.500 mg (Fe S04.H20] ZINC SULPHATE-Zn MANGANESE SULPHATE-Mn [Mn S04.H20] POTASSIUM

IODIDE(STAB)-I

COBALT CARBONATE-Co COPPER SULPHATE-Cu L SODIUM SELENITE-Se 6 7 0.00 gm 268.00 gm 3.35 gm 3.35 gm 33.50 gm 1.01 gm 100.500 mg 40.200 mg 0.502 mg 0.502 mg 5.000 mg 0.150mg USAGE: 10 Kg per Tonne Porcomega* Pig Breeder Supplement.

4. FORMULA PER TONNE PORCOMEGA. PIG BREEDER LACTATING 004 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 49/55 PORCOMEGA* BASE SP VITAMIN PREMIX PV 004 MINERAL PREMIX PM 004 CHOLINE CIILORJDE 60% 984.15 2.50 10.00 3.35 1000.00 98.415 0,250 1.000 0.335 100.000 USAGE: 150 KG PER TONNE OF PIG BREEDER LACTATING DIET 004.

PIG BREEDER LACTATING VITAMIN PREMIX PV 004

COMPOSI''ION

TNGREDTENT

ACTIVE

POTENCY/GM FORMULA /Kg PREMIX VITAMIN A-500 500,000 IU VITAMIN 03-500 500.000 1 U ViTAMIN E-50% 500 JU VITAMIN K3 1000 mg THIAMINE HYDROCHLORIDE USP892 mg RIBOFLAVIN 95% F.G. 950mg PYRIDOXINE USP 823 mg VITAMIN B 12-SUPPLEMENT 1% 10mg NIACIN USP 990 mg CALCIUM d-PANTOTHENATE USP920 mg FOLIC ACID USP 920 mg D- BIOTIN SUPPLEMENT 1% 10 mg GREEN TEA POWDER VANILLIC CRYSTALS 1000mg WHEAT MEAL CARRIER 53.60 g 5.36 gn 134.00 10.05 g 4.51 gn 14.11 g 4.88 gn 4.02 gn 54.14 g 29.13 g 2.91 gn 53.60 g 120.00i 13.40g 496.29 :m 26.800,000 IU i 2,680.000 IU gm 67,000 IU ;m 10.05 gm n 4.02 gm m 13.40 gm S 4.02 gm 40.20 mg m 53.60 gm m 26.80 gm 2.68 gm m 536.00 mg ng 120.00 gm m 13.40gm gm 1000.00 gm USAGE: 2.5 Kg per Tonne Porcomega- Pig Breeder Lactating Supplement.

PIG BREEDER LACTATING- PORCOMEGA AND COMPLETE FEED VITAMIN ADDITION

COMPOSITION

INGREDIENT'

ACTIVE ACTIVE /Tonne PORCOMEGA- /Kg COMPLETE FEED VITAMIN A-500 VITAMIN D3-500 67.000,000 IU 6,700,000 IU 10.000 IU 1.000 1U COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 50/55 VITAMIN E-50% 167,500 IU VITAMIN K3 10.05 gm THIAMINE HYDROCHLORIDE USP 10.05 gm RIBOFLAVIN 95% P.G. 33.50 gm PYRIDOXJNE USP 10.05 gm VITAMIN B12.SUPPLEMEN-I' 1% 75.00 mg NTACIN USP 134.00 gm CALCIUM (1-PANTOTIIENATE USP 67.00 gm F'OLIC ACID USP 6.70gm D-BIOTIN SUPPLEMENT 1% 134.00 mg GREEN TEA POWDER. 300.00 gm VANILLIC CRYSTALS 33.50 gm PIG BREEDER LACTATING MINERAL PREMIX PM 004.

25.0 1U 1.50mg 1.50 mg 5.00 mg 1.50mg 15.00 mg 0.00mg 10.00 mg 1.00 mg 200.00 mg 44.77 mg 5.00 mg COMPOSITION ACTIVE

INGREDIENT

FERROUS SULPHATE (Fe 304i120) ZINC SULPHATE MANGANESE SULPHATE (Mn S04-H20) POTASSIUM IODIDE(STAB) COBALT CARBONATE COPPER SULPHATE SODIUM SELENITE LIMESTONE CARRIER

POTENCY/GM

310M mg 360.0 mg 280.0 mg 68.8 mg 460.0 mg 250.0 mg 400.0 mg 1000.0 mg

FORMULA

216.125 gm /KgPREMIX 67.000 gm 186.1 10gm 67.000 gm 957 15 gm 26.800 gm 0.488 gm 0.728 gm 13.400 gm 0.25 1 gm 487.183gm 1000.000gm 0.335 gm 0.335 gm 3.350 gm 0101 gm USAGE: 10 Kg per Tonne Porcomega* Pig Breeder Lactating Supplement.

PIG BREEDER LACTATING PORCOMEGA. AND COMPLETE FEED MINERAL

ADDITION

COMPOSITION

ACTIVE ACTIVE COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 51/55 INGREDIENT /Tonne PORCOMEGA- /Kg COMPLETE FEED ~Y FERROUS SULPHATE-Fe (Fe S04. ZINC SULPHATE-Zn MANGANESE SULPHATE-MU (Mn 504.1120) POTASSIUM IODIDE(STAB)- COBALT CARBONATE-Co COPPER SULPHATE-Cu SODIUM SELENITE.Se 670.00 gm 670.00 gm 268.00 gm 3.35 gm 3.35 gm 33.50 gm 1.01 gm 100.500 mg 100.500 mg 40.200 mg 0.502 mg 0.502 mg 5.000 mg 0.150 mg USAGE:- 10 Kg per Tonne Porcomega Pig Breeder Lactating Supplement.

FORMULA PER TONNE PORCOMEGA* PIG GROWER 005 PORCOMEGA BASE SP VITAMIN PREMIX PV 005 MINERAL PREMIX PM 005 USAGE 150 KG PER TONNE OF PIG GROWER DIET 005.

PIG GROWER VITAMIN PREMIX PV 005

COMPOSITION

kg 987.50 2.50 10.00 1000.00 97.080 0.250 1.000 100.000

ACTIVE

INGREDIENT POTENCY/GM FORMULA /Kg PREMIX INGREDIENT POTENCY/GTM FORMULA /Kg PREMIX [VITAMIN A-500 VITAMIN D3-500 VITAMIN E-50% VITAMIN K3 THIAMINE IIYDROCFILORIDE

USP

RIBOFLAVIN 95% F.G.

PYRIDOXINE USP VITAMIN B 12 Supplement 1% NIACIN USP CALCIUM d-PANTOTHENATE

USP

500,000 IU 500,000 IU 500 IU 1000 mg 892 mg 950 mg 823 mg 10 mg 990 mg 920 mg 40.20 gm 8.04 gm 53.60 gm 2.68 gm 3.00 gm 7.05 gm 4.88 gm 2-68 gm 27.07 gm 23.30 gm 20.100.000 IU 4.020,000 IU 26,800 IU 2.68 gm 2.68 gm 6.70 gm 2.68 gm 26.80 gm 26.80 gm 21.44 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 52/55 FOLIC ACID USP D-BIOTIN SUPPLEMENT 1% GREEN TEA POWDER

CRYSTALS

WHEAT MEAL CARRIER 920mg 10mg 1000 mg 1000 mg 1.46 gm 13.40 gm 120.00 gm 6.70 gm 760.40 gm 1.34 gm 134.00 mg 120.00 gm VANTLLIC 170 gm 1000.00 gm USAGE: 2.5 Kg per Tonne Porcomega Pig Grower Supplement 005.

PIG GROWER PORCOMEGA' AND COMPLETE FEED VITAMIN ADDITION

COMPOSITION

ACTIVE

ACTIVE

/Tonne PORCOMEGA' /Kg COMPLETE FEED VITAMIN A-500 VITAMIN D3-500 VITAMIN E-50% VITAMIN 1(3 THIAMINE HYDROCHLORIDE USP RIBOFLAVIN 95% F.G.

PYRIDOXINE USP VITAMIN B 12-SUPPLEMENT 1% NIACIN USP CALCIUM d-PANTOTHENATE USP FOLIC ACID USP D-BIOTIN SUPPLEMENT 1% GREEN TEA POWDER VANILLIC CRYSTALS 50,250.000 IU 10,050,000 IU 67.000 IV 6.70 gm 6.70 gm 16.75 gm 6.70 gm 67.00 mg 67.00 gm 53.60 gm 335 gm 335.00 mg 300.00 gm 16.75 gm 7,538 IU 1,508 IU 1.00 mg 1.00 mg 2.51 mg 1.00 mg 10.05 mg 10.05 mg 8.04 mg 0.50 mg 50.25 mg 45.00 gm 2.51 mg PIG GROWER MINERAL PREMIX PM 005

COMPOSITION

ACTIVE

INGREDIENT POTENCY/GM FORMULA /KgPREMIX FERROUS SULPHATE (Fe S04.H20) ZINC SULPHATE MANGANESE SULPHATE 310Mm 360.0 mg 280.0 mg 21(1125 gm 186.110 gm 95.715 gm 67.000gm 67.000 gm 26.800 gm COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/20OG 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 53/55 (Mn S04 POTASSIUM IODIDE(STAB) COBALT CARBONATE COPPER SULPHATE SODIUM SELENTTE LIMESTONE CARRIER 68.8 mg 460.0 mg 250.0 mg 400.0 mg 1000.0 mg 0.488 gm 0.728 gm 13.

4 00 gm 0.25 1 gm 487. 183 gm 0335 gm 0.335 gm 3.350 gm 0.101 gm 487.183 gm 1000.000 gm USAGE: 10 K- per Tonne Porcomega- Pig Grower Supplement 005.

PIG GROWER PORCOMEGA* AND COMPLETE FEED MINERAL ADDITION

COMPOSITION

INGREDIENT

FERROUS SULPHATE-Fe (Fe S04H20) ZINC SULPHATE-Zn MANGANESE SULPHATE-Mn (Mn S04.H20) POTASSIUM

IODIDE(STAB)-I

COBALT CARBONATE-Co COPPER SULPFIATE-Cu SODIUM SELENITE-Se

ACTIVE

ACTIVE

Tonne PORCOMEGAS 670.00 gm 670.00 gm 268.00 gm 3.35 gm 3.35 gm 33.50 gm 1.01 gm /Kg COMPLETE FEED 100.500 mg 100.500 mg 40.200 mg 0.502 mg 0.502 mg 5.025 mg 0.151 mg 6. FORMULA PER TONNE PORCOMEGA' PIG FINISHER 006 PORCOMEG BASE SP VITAMIN PREMIX PV 006 MINERAL PREMIX PM 006 kg 987.50 2.50 10.00 1000.00 97.080 0.250 1.000 100.000 USAGE 150 KG PER TONNE OF PIG FINISHER DIET 006.

PIG FINISHER VITAMIN PREMIX

PVOOG

COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 54/55

COMPOSITION

INGREDIENT

ACTIVE

/Kg PREMIX POTENCY/Gm FORMULA VITAMIN A-500 500.000 IU VITAMIN D3-500 500.000 TU VITAMIN E-50%/ 500 1U VITAMIN K3 1000mg TIIAMINE I-IYDROCHLORIDE USP 892 mg RIBOFLAVIN 95% F.G. 950mg PYRIDOXINE USP 823 mg VITAMIN B 12.SUPPLEMENT 1% 10 mg NIACIN USP 990 mg CALCIUM D-PANTOTHENATE USP 920 mg FOLIC ACID USP 920 mg D-BIOTIN-SUPPLEMENT 1% 10 mg GREEN TEA POWDER 1000 mg VANILLIC CRYSTALS 1000 mg WHEIAT MEAL (CARRIER) 1000 mg 26M0 gm 5.36 gm 53MOgm 2.68 gmi 3-00 gm 5.64 gm 4.88 gm 1.34 gm 18.95 gm 20.39 gm 1.46 gmn 1340 gm 120.00 gm 4.02 gm 760.40 gm 1000.00gm 13.400.000 IV.

2.680.000 IU.

26.800 1U.

2.68 gm 2.68 gm 5.36 gm 2.68 gm 13.40 mg 18.76 gm 18.76 gm 1.34 gm 134.00 mg 120.00 gm 4.02 gm USAGE: 2.5 Kg per Tonne Pcrcomega Finisher Supplement 006.

PIG FINISHER PORCOMEGA* AND COMPLETE FEED VITAMIN ADDITION

COMPOSITION

ACTIVE ACTIVE

INGREDIENT

-VITAMIN A-500 VITAMIN D3-500 VITAMIN E-50% VITAMIN K3 THIAMINE HYDROCHLORIDE

USP

RIBOFLAVIN 95% F.G.

PYRIDOXINE USP VITAMIN B12-SUPPLEMENT 1% NIACIN USP CALCIUM d-PANTOTHENATE

USP

/Tonne PORCOMEGA* /Kg COMPLETE FEED 33.500,000 IU 5,025 1U 6,700.000 TI 1.005 IU 67,000 IU 10.05 IU 6.70 gm 1.01 mg 6.70 gm 1.01 mg 13.

4 0 gm 2.01 mg 6.70 gm 1.01 mg 3350mg 5.03 mg 4 6.90gm 7.04 mg 46.90 gm 7.04 mg COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 55/55 FOLIC ACII) USP D-BIOTIN SUPPLEMENT 1% GREEN TEA POWDER VANILLIC CRYSTALS 3.35 gm.

335.00 mg 300 gm 10.05 gm 0.50 mg 50.00 meg 45.00 mg 1.51 mg PIG FINISHER MINERAL PREMIX PM 006 COMPOSITION ACTIVE INGREDIENT POTENCY/GM FORMULA /KgPREMIX FERROUS SULPHATE (Fe S04112) ZINC SULPHATE MANGANESE SULPHATE (Mn 804.1120) POTASSIUM IODJDE(STAB) COBALT CARBONATE COPPER SULPHATE SODIUM SELENITE LIMESTONE CARRIER 310.0 mg 216.125 grn 360.0 mg t86.110 gm 280.0 mg 957 15 gm 68.8 mg 460.0 nm 2 5 0.0 mg 400-0 rug 1000.0 mg 67.000gm 87.000gm 26.800 gm 0.335 gm 0.335 gm 3.350 gm 0,101 gm 165.330 gm 0.488 gm 0.728 gm 13.400 gm 0.251 gm 5.330 gm 1000.000 gm USAGE: 10 Kg per Tonne Porcomega. Finisher Supplement 006 PIG FINISHER PORCOMEGA. AND COMPLETE FEED MINERAL ADDITION

COMPOSITION

ACTIVE

ACTIVE

.11 INGREDIENT /Tonne PORCOMEGA FERROUS SULPHATE .Fe 670.00 gm (Fe S04H20) ZINC SULPHATE Zn 670.0 gm MANGANESE SULPI-ATE. .Mn 268.00 gm (Mn S04.H20) POTASSIUM IODIDE(STI~).I 3.35 gm COBALT CARBONATE.CO 3.35 gm COPPER SULPFIATE.CU 33.50 grn SODIUM SELENITE. Se 1.01 gm /kg COMPLETE FEED 100.500 rug 100.500 mg 40.200 mg 0.502 mg 0502 mg 5025 mg 0.15 1 mg COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21

Claims (5)

1. 3. An animal feed supplement according to claim 1, wherein the at least one naturally- occurring antioxidant is at least one selected from the group consisting of Vitamin E, Carotenoids, Vitamin C, Rosemary oil, Thyme white oil, Peppermint oil, Jasmine tea, Sodium Selenite, Crude Canola oil, and combinations thereof.
2. 4. An animal feed supplement according to claim 1, wherein the antibacterial agent is at least one selected from the group consisting of Potassium Sorbate food acid, Citric Acid food acid, Benzoic Acid food preservative, Phosphoric acid, and combinations thereof. 4] COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 0I Inc /11)mno 1) In b.o -z-95z84 WALSH AND ASSOCIATES PAGE 06/55 00 0 0 ci An animal feed supplement according to claim 1, wherein the anti-chemical agent is at least one selected from the group consisting of Phosphoric Acid, Potassium Sorbate, Citric Acid, and combinations thereof
3. 6. An animal feed supplement according to claim I, wherein the supplement includes a blend of: o fish meal, 00 an oil based premix, 0 a dry mix premix, and a water-based green tea extract. 7 An animal feed supplement according to claim 6, wherein the oil based premix comprises a blend of the following ingredients: Ethoxyquin vitamin grade; Crude palm oil; Crude Canola oil Carrier; Aniseed China Star; Rosemary oil; Thyme white oil; Peppermint oil; Benzoic Acid; and Phosphoric Acid 81%. 8 An animal feed supplement according to claim 6, wherein the dry mix premix comprises a blend of the following ingredients: Vitamin E; Vitamin C; Citric Acid; Propyl Gallate; Calcium Propionate; and Wheat Meal Carrier. 42 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 07/55 9 An animal feed supplement according to claim 6, wherein the fish meal, oil based premix, dry mix premix and water based green tea extract are blended in the following proportions: Fish Meal Oil based premix Dry mix Premix Water based infused green tea extract kg
4. 983.00 10.00 5.00 2.00
5. 1000.00 98,300 1.000 0.500 0.200 100.000. (Original) An animal feed supplement according to claim 9, wherein the constituents of the oil based premix per Ion of a food base are blended in the following proportions: kg Ethoxyquin vitamin grade 0.400 4.00 Crude palm oil 1.500 15.00 Aniseed China Star 0.067 0.67 Rosemary oil 0.100 1.00 Thyme white oil 0.100 1.00 Peppermint oil 0.150 1.50 Benzoic Acid 0,005 0.05 Phosphoric Acid 81% 0.250 2.50 Crude Canola oil Carrier 7.428 74.28 10.000 100.00. 11 An animal feed supplement according to claim 10, wherein the mix premix per ton of food base are blended in the following proportions: Vitamin E 0.800 Vitamin C 0.500 constituents of the dry 16.00 10.00 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 23:18 +bl-2-95522894 WALSH AND ASSOCIATES PAGE 08/55 00 0 0 Citric Acid 0.500 10.00 Propyl Gallate 0.100 2.00 Cl Calcium Propionate 0.500 10.00 Wheat Meal Carrier 2.600 52.00 5.000 100.00. O 12. An animal feed supplement according to claim 11, wherein the Vitamin C and 00 Citric Acid are scavenger antioxidants. 0 0 c 13 An animal feed supplement according to claim 1, wherein the supplement supplies the omega 3 long chain fatty acids Docosahexaenoic Acid, Docosapentaenoic acid, and Eicosapcntaenoic acid to the animal. 14 An animal feed supplement according to claim 1, wherein the percentage of feed supplement comprises 15% of the total dietary intake of the animal. An animal feed supplement according to claim I, wherein the animal produce comprises pork, poultry meat, lamb, eggs, beef, or farmed fish. 16. An animal feed supplement according to claim 1, wherein the anti-mold anti- fungal agent comprises calcium propionate. 17. An animal feed supplement according to claim 16, wherein the fish meal comprises tuna meal, and wherein the tuna meil, oil based premix, dry mix premix, and water based green tea extract comprise the following constituents: Tuna Meal; Ethoxyquin vitamin grade; Propyl Gallate; Vitamin C; Crude palm oil; 44 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 09/55 00 0 0 Cl ;Z Crude Canola oil Carrier; -s Aniseed China Star; Cl Rosemary oil; Thyme white oil; Peppermint oil; Vitamin E C"l O Benzoic Acid; C"l 00 Phosphoric Acid 81%; 0 SCitric Acid; C, Calcium propionate; Water based green tc:a extract; and Wheat Meal Carrier. 18. An animal feed supplement according to claim 17, wherein the tuna meal, oil based premix, dry mix premix, and water based green tea extract are blended in the foIlowing proportions per ton of food base: kg Tuna Meal 985.000 98.500 Ethoxyquin vitamin grade 0.400 0.040 Crude palm oil 1.500 0.150 Crude Canola oil Carrier 7.428 0.743 Vitamin C 0-500 0.050 Propyl Gallate 0.100 0.010 Aniseed China Star 0.067 0.007 Rosemary oil 0.100 0.010 Thyme white oil 0.100 0.010 Peppermint oil 0.150 0-015 Vitamin E 50 0.800 0.080 Benzoic Acid 0.005 0.001 Phosphoric Acid 81% 0.250 0.025 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 10/55 00 0 0 Citric Acid 0.500 0.050 Calcium Propionate 0-500 0.050 Cl Water based green tea extract 2.00 0.200 Wheat Meal Carrier 2.600 0.260 1000.000 100.00. C1 O 19 An animal feed supplement according to claim 18, wherein the supplement further 00 comprises the following constituent proportions: Cl Vanillic Crystals 0.013. A method of feeding animals using a fish-meal-based feed supplement, comprising: 46 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/20088 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 11/55 00 O O C] a) selecting a fish meal source of omega 3 long chain fatty; c) selecting at least one synthetic antioxidant; d) selecting at least one naturally-occurring antioxidant e) selecting an antibacterial agent; f) selecting an anti-mold anti fungal agent; and C g) selecting an anti-chemical activity agent; CI h) selecting a percentage of the feed supplement greater than 5% of the total 0, dietary intake of the animal; 0 wherein the feed supplement is included in the diet of an animal so that its 0 quantity is greater than 5% of the total dietary intake of the animal; and wherein the omega 3 long chain fatty acids quantities increase as percentage above 5% increases the nutritional value of produce from animals to whom the supplement is fed but without adverse effects to the physical and sensory qualities of the food produced from animals to whom the supplement is fed. 21 A method according to claim 20, further comprising selecting the at least one synthetic antioxidant from one or a combination of the following: tert-butylhydroquinone; butylated hydroxyanisole, butylated hydro ytoluene; Ethoxyquin vitamin grade; Propyl Gallate; Ascorbic acid; Crude palm oil; and Crude Canola oil. 22 A method according to claim 21, further comprising selecting the at least one naturally-occurring antioxidant from one or a combination of the following: Vitamin E; Carotenoids; Vitamin C; 47 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 12/55 00 O O SRosemary oil; ;Z Thyme white oil; Peppermint oil; Jasmine tea; and Sodium Sclcnite. C 23 A method according to claim 22, further comprising selecting the antibacterial 0 agent from one or a combination of the following: 00 Potassium Sorbae food acid; oCitric Acid food acid; Benzoic Acid food preservative; and Phosphoric acid. 24 A method according to claim 23, further comprising selecting the anti- chemical agent from one or a combination of the following: Phosphoric Acid; Potassium Sorbate; and Citric Acid. A method according to claim 24, further comprising forming an oil based premix by blending the following constituents: Ethoxyquin vitamin grade; Crude palm oil; Crude Canola oil Carrier; Aniseed China Star; Rosemary oil; Thyme white oil: Peppermint oil; Benzoic Acid; and Phosphoric Acid 81 48 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:19 +61-2-95522894 WALSH AND ASSOCIATES PAGE 13/55 00 0 0 S26 A method according to claim 25, further comprising forming a dry mix premix Sfrom the following ingredients: Vitamin E; Vitamin C; Citric Acid; en Propyl Gallate; C Calcium Propionate; and Wheat Meal Carriers. 00 0 O 27. A method according to claim 26, comprising blending the fish meal, the oil based premix, the dry mix premix, and a water based green tea extract together. 28. A method according to claim 27, further comprising mixing the fish meal, oil based premix, dry mix premix and water based green tea extract in the following proportions: kg Fish Meal 983.00 98.300 Oil based premix 10.00 1.000 Dry mix Premix 5.00 0.500 Water based infused green tea extract2.00 0.200 1000.00 100.000. 29. A method according to claim 28, further comprising blending the oil based premix per ton of a food base in the following proportions: Kg °A Ethoxyquin vitamin grade 0.400 4.00 Crude palm oil 1.500 15.00 Aniseed China Star 0.067 0.67 Rosemary oil 0.100 1.00 Thyme white oil 0.100 1.00 Peppermint oil 0.150 1.50 49 COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21 21/06/2008 23:18 +61-2-95522894 WALSH AND ASSOCIATES PAGE 14/55 00 O O (N SBcnzoic Acid 0.005 0.05 Phosphoric Acid 81% 0.250 2.50 Crude Canola oil Carrier 7.428 74.28 10.000 100.00. n 30 A method according to claim 29, comprising blending the dry mix premix C, per ton of food base in the following proportions: C kg 00 Vitamin E 50 0.800 16.00 0 Vitamin C 0.500 10.00 Citric Acid 0.500 10.00 Propyl Gallate 0.100 2.00 Calcium Propionate 0.500 10-00 Wheat Meal Carrier 2.600 52.00 5.000 100.00 31 A method of preparing a feed supplement, comprising: a) selecting fish meal as a source of omega 3 long chain fatty acids; b) preparing a dry mix premix and an oil based premix; c) blending the fish meal, the oil based premix, the dry mix premix and a water based green tea extract, in predetermined proportions of the supplement; d) selecting additives for the supplement appropriate to the stage of development of the animal to which the supplement is fed, and c) blending the additives with the fish meal, dry mix premix, oil based premix and water based green tea extract; wherein the supplement is within the range of 5%-20% of an animal's total diet, and wherein animal produce when fed with the supplement prior to slaughter is free from fishy taint after slaughter. 32 A method according to claim 31, wherein the feed supplement comprises of the dietary intake of the animal. COMS ID No: ARCS-195426 Received by IP Australia: Time 23:02 Date 2008-06-21