AU2008261185B2 - Biologically active extract from dendrobium plant, use thereof and process for preparing the same - Google Patents

Abstract

BIOLOGICALLY ACTIVE EXTRACT FROM DENDROBIUM PLANT, USE THEREOF AND PROCESS FOR PREPARING THE SAME A substance having the following formula is provided, wherein R1 and R2 are identical or different functional groups. The 10 substance has an effect of accelerating a phagocytosis of a retinal pigment epithelium (RPE) cell.

Description

Regulation 3.2 AUSTRALIA Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT APPLICANT: NATIONAL YANG-MING UNIVERSITY Invention Title: BIOLOGICALLY ACTIVE EXTRACT FROM DENDROBIUM PLANT, USE THEREOF AND PROCESS FOR PREPARING THE SAME The following statement is a full description of this invention, including the best method of performing it known to me: BIOLOGICALLY ACTIVE EXTRACT FROM DENDROBIUM PLANT, USE THEREOF AND PROCESS FOR PREPARING THE SAME [00011 This invention relates to a process for preparing a biologically 5 active extract from a plant and the extract thereof, and especially to one process of using a water miscible organic solvent or a mixture thereof with water for obtaining an extract from the plant. [0002] As described in US Patent No. 7,101,577, it is known that the Dendrobium species is considered to be the most precious Chinese herb for 10 treating ophthalmic defects. According to our previous research experience, it appears that the Herba Dendrobii (which, in tradition, is the generalized name for the Dendrobium plants having the curative effects) has the most biological effects. However, in recent years, it appears there is a trend of that the Dendrobium plants having the curative effects on their stems are generally 15 named as Caulis Dendrobii and the Dendrobium plants having the curative effects on their whole plants are generally called as Herba Dendrobii. [0003] Based on the descriptions in the mentioned application, it is known retinal pigment epithelium (RPE) is a monolayer cell at the surface layer of the retina, which is located between the Bruch's membrane and the 20 photoreceptors. Since the RPE can effectively remove or transmit the toxic materials and the metabolite of the choroid coat and the retina, it performs a very important blood-retinal barrier. In addition, the RPE has many functions, such as receiving light, phagocytizing the outer segments separated from the rod cell and the cone cell because of light stimulation, catabolizing the 25 phagosome, synthesizing the extracellular matrix and the melanin, detoxifying 1 the medicine, providing the essential material for reproducing the outer segments of the photoreceptor, storing and transmitting the Vitamin A, synthesizing the rhodospin, and forming the adherent force of the retina. The normal phagocytosis of the RPE plays a critical role in maintaining the health 5 of the photoreceiptors in the retina. Once the function of phagocytosis is reduced, it will result in the degeneration of the photoreceptors. Although the RPE will be dead or moved to someplace else with the increasing age, the aged RPE still owns the phagocytic ability. Further, the lost quantity of the rod cells are more than those of the cone cells, which causes the diseases and the 1o vision degradation of the aged people. Therefore, maintaining the RPE function is quite important for the visional system. [0004] In the immune system, the NO plays a defensive role and is toxic to cells. In the blood vessel system, the NO is a so-called endothelium derived relaxing factor (EDRF). And, in the central nervous system, the NO 15 acts as a neurotransmitter. [00051 The NO is released from the process of transferring L-arginine into L-citrulline via a nitric oxide synthase (NOS). The NOS includes three kinds of isoforms, a neuronal NOS, an endothelial NOS and an immunologic NOS. The neuronal NOS and the endothelial NOS are constitutive forms, 20 named as cNOS, whose activities are regulated by the calcium ion (Ca") and the calmodulin, and the concentration of the released NO is in the level of nano-molarity (nM). The immunologic NOS is an inducible form, named as iNOS, whose activity is not regulated by the Ca" and the calmodulin, and the concentration of the released NO is in the level of milli-molarity (mM). The 2 genes of the cNOS and the iNOS are respectively located on different chromosomes. [0006] In retina, the NOS has been found in the retinal neuron, RPE, amacrine cells, ganglion cells, and Muller cells. It appears that the NO plays 5 an important role in the physiology and pathology, and is closely related to the functions of the eye. [00071 It's found that the NO owns the ability of regulating the blood flow of the retina under a basal condition or an ischemia environment. Further, it's believed that the NO may own the ability of regulating the damage 1o degrees of the blood vessels in the retina, in which the damage is caused by diabetes. In addition, when the retinal glial cells and the RPE are stimulated by the LPS, IFN-g, and the TNF-a, the NOS will be largely expressed, which largely increase the production of the NO. In other words, under the conditions that the retina is inflamed or infected, the NO might play a role in 15 the defense and protection mechanisms. [00081 Till now, the position and the characteristics of the cNOS in the photoreceptor are still unclear. Some references disclose that the main body of the photoreceptor has the cNOS activities, and other references disclose that only the photoreceptor outer segments own the cNOS activities. The released 20 NO can regulate the transmission of light, the transmitted message of the neutron synapase, and the blood flow of the retina under a physiological condition or an ischemia environment. The iNOS activity can also be found in some cells in the retina, such as the RPE and the Muller cells. In the culture of a bovine RPE, after being stimulated for 12 hours with the IFN-y, 25 LPS, and TNF-a, a mass of NO will be released for at least 96 hours. The 3 effects of the cytokines on the RPE iNOS activity are quite complex. In a bovine RPE, for instance, being stimulated by the LPS and the IFN-y, or the IFN-a are necessary for releasing a mass of NO. The bFGF inhibits the functions of NOS, but the TGF-Pslightly enhances the functions of the NOS. 5 For a human RPE, it is necessary to be stimulated by the Interleukin-l pto release a mass of NO. However, the LPS is not the necessary factor to stimulate a human RPE. In addition, the TGF-$ obviously inhibits the release of the NO in a human RPE. [00091 When infected by bacteria, the expressions of iNOS may be 10 beneficial because the released NO will kill the invaded microorganism. Contrarily, in some cases, when the released NO is exceeded, the released NO will result in the autoimmune diseases or the septic shock. In 1994, the first evidence for explaining the relationship between NO and the inflammation of the fundus oculi is proposed, and the reference also proposed that the uveitis 15 resulted from the endotoxins can be blocked by the iNOS inhibitor. On the other hand, it appears that the aFGF and the bFGF can inhibit RPE from generating a mass of NO by treating the RPE with IFN-yand LPS. Since it is the expression of the iNOS, but not the stabilities of the iNOS being mRNA, is inhibited, it's conjectured that the FGF will protect the RPE from being 20 damaged by the endotoxins and the cytokines. Thus, it can be seen that the iNOS also plays a role in regulating the immunity of the retina. [00101 The common retinal diseases include the proliferative diabetic retinopathy caused by the diabetes, the proliferative vitreoretinopathy, and the Aged-macular degeneration. However, the retinal diseases are the hardest 25 diseases to cure in the ophthalmic defects. The hyperglycemia accelerates the 4 glycation, which forms the advanced glycation end products (AGEs), and it is believed that the AGES closely relates to the vascular complication or the neuronal complication. An unstable schiff base is formed via the nonenzymatic reaction between the aldehyde group or the ketone group of the 5 reducing sugar and the primary amino acids of the protein. Then, an amadori product is formed from the schiff base via the amadori rearrangement. And, the advanced glycosylation end product (AGE) will be formed from the amadori product via the rearrangement process. It is known that the nonenzymatic glycosylation is not a reversible reaction and usually occurs at 10 the protein having a long half-life. While the AGE formation results in cross-linking, the protein molecule would have a resistance to the protease. Therefore, the accumulation of the AGE would be an aging mark. With the increasing age, the AGE amounts in the pyramidal neurons of the brain, the Bruch's membrane and the collagen will increase gradually. The reactive rate 15 of the nonenzymatic glycosylation is a primary reaction, and the reaction rate is dependent on the concentrations of the reducing sugar and the protein. Usually, a diabetic patient has a higher blood glucose concentration than normal people, so that the glycosylation situation will be increased. It is known that the diabetic patients have higher probabilities of having some 20 diseases or symptoms for the normal people are all directly related to the AGE, in which the disease or symptoms include the atherosclerosis, the kidney impair, the vessel damage, the neuron disease, the retinopathy, and the apoplexy. The main reason for the aggregation of the erythrocyte, resulted from the diabetes, is that the tertiary structure of the albumin is changed after 25 being glycosylated, so that the glycosylated albumin loses the functions of the 5 anti-aggregation. Further, the reason for changing the permeability of the glomerulus is the glycosylation of the albumin but not the glycosylation of the glomerular basement membrane. In addition, the glycosylated protein has a better ability for penetrating the blood brain barrier. 5 [0011] The AGE can combine with some receptors on the cell surface or some proteins. The known receptors include the scavenger receptors type I, the scavenger receptors type II, the receptor for AGE (RAGE), OST-48 (AGE-Ri), 80K-H phosphoprotein (AGE-R2), and the galectin-3 (AGE-R3). Besides, the RAGE can be found at the surfaces of the monocyte, the 10 macrophage, the endothelial cell, and the glia cell. When the cell is activated by the AGE, the expressions of the extracellular matrix protein, the vascular adhesion molecules, and the growth factors will increase. Depending on the different cell types and the transmitted signals, some phenomena will occur accompanied with the above situation, such as the chemotaxis, the 15 angiogenesis, the oxidative stress, the cell proliferation and the programmed cell death. It appears that the various cells in the human brain are able to express different RAGEs, which remove the AGE. When the remove ability is lost, the AGE will be accumulated outside the cell, which induces the inflammation reaction of the central nervous system. Furthermore, the AGE 20 will induce the expressions of both the retinal vascular endothelial growth factor of the RPE and the PDGF-p. The AGE plays an important role in the aging process, so that designing a pathological model by the glycosylated albumin for developing a new medicine is very important. [0012] The most important growth factor in the liver is the hepatocyte 25 growth factor/ scatter factor (HGF/SF), which is formed by combining the 60 6 KDa heavy chain (achain) with the 30 KDa light chain chaini) through the disulfide bond. The newly formed HGF/SF is the prepro HGF/SF, which needs to be modified by an enzyme for forming the heterodimeteric form before having a biological activity. The HGF is a multi-function growth 5 factor, which not only has the ability for regulating the growth of the various cells, but also plays an important role in the tissue repair and the organ regeneration. The internal distribution of the HGF is very extensive, wherein the liver has the highest quantity of the HGF. Furthermore, the HGF can be found in the pancreas, the thymus, the blood, the small intestines, the placenta io and so forth. In addition, the HGF/SF or the HGF/SF receptors are found in the eye secretions and the eye tissues, such as the tears, the lachrymal gland, and the cornea, so that it is conjectured that the HGF may play a role in the regulation of eyes. Besides, it's known that the RPE has both the HGF and the HGF receptor (c-Met). Since the tyrosine phosphorylation of the c-Met 15 expresses all the time, the HGF may be a growth factor with the self-stimulation function for the RPE. Further, the HGF may be related to the development of the retina, the wound healing, and the newborn retinal vessels. [0013] From the above, it is known that RPE plays an important role in retinal regulation mechanisms. Meanwhile, it has been proved that the 20 Chinese herb, Dendrobium species, is able to enhance or inhibit some functions or regulation mechanisms in RPE. More specifically, the Dendrobium species can enhance the expressions of RPE phagocytosis, the NO formation of the RPE, the gene expressions of the RPE liver hepatocyte growth factor. The Dendrobium species can inhibit the gene expressions of 25 the bFGF, the VEGF and the TGF-p in the RPE under a normal condition and 7 an ischemia environment. Consequently, the relevant researches about the enhancing factors of the RPE activities are important for improving the health of the body. In the preceding researches, it has known that the extract from Herba Dendrobii has an effect of accelerating a phagocytosis of RPE and 5 increases the AGE degradation. In order to further control the effect of accelerating the phagocytosis of RPE, further understanding about the extract from Herba Dendrobii is desired. [0014] In accordance with an aspect of the present invention, a composition having a substance with structure of formula 1 is provided, OH

R

2 8 HO O R1 6 13 5 10 OH O formula 1 wherein R, and R 2 are the same or different functional groups. [0015] Preferably, the composition has a physiological activity. [0016] Preferably, the composition has an effect of accelerating a phagocytosis of a retinal pigment epithelium (RPE) cell. 15 [0017] Preferably, the substance is one selected from a group consisting of an apigenin 6,8-Di-C-ax-L-arabinopyranoside, an apigenin 6-C-P-D Xylopyranosyl-8-C-a-L-arabino-pyranoside, an apigenin 6-C-a-L-Arabino pyranosyl-8-C-P-D- arabinopyranoside and a combination thereof. 8 [00181 In accordance with another aspect of the present invention, a substance with a structure of the formula 1 is provided, wherein either R 1 or R 2 is one selected from a group consisting of a-Ara, p-Ara and P-Xyl. [0019] Preferably, the substance has an effect of accelerating a 5 phagocytosis of an RPE cell. [00201 In accordance with a further aspect of the present invention, a substance with a structure of the formula 1 is provided, wherein either of the

R

1 and R 2 is one selected from a group consisting of c-Ara, p-Xyl, p-Ara, c-6-Deoxy-xylo-hexos-4-ulosyl, p-Glu, p-Gal, a-Rha, p-Ribose, ax-Arabinose, 10 p-Arabinose, p-Xylose, P-Lyxose, p-Allose, p-Altrose, p-Mannose, p-Gulose, p-Idose, p-Talose, P-Tagatose, p-Fructose, p-Glucose, p-Galactose, and a-Rhamnose. [00211 Preferably, R 1 and R 2 are identical or different and either R 1 or R 2 is one selected from a group consisting of C-P-D-glucopyranosyl, 15 C-p-D-galactopyranosyl, C-p-D-xylopyranosyl, C-p-D-arabinopyranosyl, C-at-L-arabinopyranosyl, C-a-L-rhamnopyranosyl and C-6-deoxy-xylo-hexos 4-ulosyl. [00221 In accordance with another further aspect of the present invention, a physiologically active composition, including a substance according to one 20 of Claims 4 and 5, is provided, wherein the substance has an effect of accelerating a phagocytosis of an RPE cell. 9 [00231 Preferably, the physiologically active composition further includes a physiologically acceptable carrier. [0024] Preferably, the physiologically active composition is a pharmaceutical composition. 5 [0025] Preferably, the physiologically acceptable carrier is a pharmaceutical carrier. [0026] Preferably, the physiologically active composition has a pharmaceutical activity on an disorder, wherein the disorder is one selected from a group consisting of RPE degeneration, age-related macular 10 degeneration, photoreceptor degeneration, diabetes, arteriosclerosis, vascular damage, retinal neuropathy, amacrine cell dysfunction, retinal ganglion cells degeneration, MUller cell dysfunction, uveitis, choroidal neovascularization, proliferative retinopathy, retinitis, Bruch's membrane disorder and a complication thereof. 15 [0027] In accordance with another further aspect of the present invention, a method for enhancing an activity of an RPE cell is provided. The method includes a step of administering a substance according to one of Claims 4 and 5. [0028] In accordance with another further aspect of the present invention, 20 a method for accelerating a phagocytosis of a retinal pigment epithelium (RPE) cell is provided. The method includes a step of administrating a substance according to one of Claims 4 and 5. [00291 In accordance with another further aspect of the present invention, a method for a treatment and/or prevention of an RPE cell-related disorder is 10 provided. The method includes a step of administrating a substance according to one of Claims 4 and 5. [00301 Preferably, the RPE cell-related disorder is one selected from a group consisting of RPE degeneration, age-related macular degeneration, 5 photoreceptor degeneration, diabetes, arteriosclerosis, vascular damage, retinal neuropathy, amacrine cell dysfunction, retinal ganglion cells degeneration, MUller cell dysfunction, uveitis, choroidal neovascularization, proliferative retinopathy, retinitis, Bruch's membrane disorder and a complication thereof. [0031] The above objects and advantages of the present invention will io become more readily apparent to those ordinarily skilled in the art after reviewing the following detailed descriptions and accompanying drawings, in which: [0032] Fig. 1 is the flow chart of separating protocol for Herba Dendrobii extract according to a preferred embodiment of the present invention; 15 [0033] Fig. 2 is the LC-MS chromatography of DCMPbL6,7D2H2H3; [0034] Fig. 3 is the HPLC chromatography of DCMPbL6,7D2H2H3; [0035] Fig. 4 is the LC-MS chromatography of DCMPbL6,7D2H2H3H2; [0036] Fig. 5 is another LC-MS chromatography of DCMPbL6,7D2H2H3H2; 20 [0037] Fig. 6 is the LC-MS chromatography of DCMPbL6,7D2H2H3H3; [00381 Fig. 7 is the UV spectrum of the methanol solution of DCMPbL6,7D2H2H3H4; [0039] Fig. 8 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3 on the total phagocytosis of RPE; 25 [0040] Fig. 9 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3 on the ingestion phagocytosis of RPE; 11 [0041] Fig. 10 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H2 on the total phagocytosis of RPE; [0042] Fig. 11 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H2 on the ingestion phagocytosis of RPE; 5 [0043] Fig. 12 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H3 on the total phagocytosis of RPE; [0044] Fig. 13 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H3 on the ingestion phagocytosis of RPE; [0045] Fig. 14 is the bar chart illustrating the effects of 10 DCMPbL6,7D2H2H3H4 on the total phagocytosis of RPE; and [0046] Fig. 15 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H4 on the ingestion phagocytosis of RPE. [0047] The present invention will now be described more specifically with reference to the following embodiments. It is to be noted that the 15 following descriptions of preferred embodiments of this application are presented herein for purpose of illustration and description only; it is not intended to be exhaustive or to be limited to the precise form disclosed. [0048] Preparation of the Herba Dendrobii extract and separation of the purified active content thereof. 20 [0049] Please refer to Fig. 1. Fig. 1 is the flow chart of the separating protocol for Herba Dendrobii extract according to a preferred embodiment of the present invention. Three active components are separated from Herba Dendrobii. 1.9 kg Herba Dendrobii is extracted by methanol three times for obtaining the methanol extract of Herba Dendrobii. The methanol extract of 25 Herba Dendrobii is then re-concentrated and completely dried for forming the 12 DCM standard. The dry DCM standard is dissolved in 2 L EtOAc and then partitioned with 2 L water for obtaining an EtOAc layer and a first water layer. The water layer is extracted with 2 L EtOAc two more times. All the EtOAc layers are collected, re-concentrated and completely dried for obtaining the 5 EtOAc extract. The dry EtOAc extract is respectively partitioned three times with 4 L hexane and 2 L methanol for obtaining a hexane layer and a methanol layer. After re-concentrated and dried, the dried hexane layer and methanol layer are named as DCMPe/h standard (Pe/h), and DCMPe/m standard (Pe/m) respectively. In addition, the first water layer is adjusted into 2L volume by 10 adding de-ionized water, and then is partitioned by adding 2 L butanol for obtaining a butanol layer and a second water layer. After re-concentrated and dried the dried butanol layer and the second water layer are named as DCMPb standard (Pb), and DCMPw standard (Pw) respectively. The Pb is extracted by LH20 gel chromatography with a molecule column (2.5x107 cm, mobile 15 phase <methanol:H 2 0=50:50>). After the activity screening, the DCMPbL6,7 samples are obtained. Then, the samples DCMPbL6,7 are extracted by the Diaion SP-20 SS chromatography with y a absorption column (1 x 30 cm). When the mobile phase is isopropanol : H 2 0 = 20 : 80, the eluate named as DCMPbL6,7D2 can be obtained. When the mobile phase is 20 isopropanol : H20= 30 : 70, the eluate named as DCMPbL6,7D3 can be obtained. When the mobile phase is isopropanol : H20 = 40 : 60, the eluate named as DCMPbL6,7D4 can be obtained. Then, the DCMPbL6,7D2 is chromatographed by HIPLC reverse C18 column (10 x 300 mm) with the mobile phase of methanol : H 2 0: acetic acid = 35:65:1, and the eluate named 25 as DCMPbL6,7D2H2 is obtained. 13 [00501 Based on our preceding researches, it is known that DCMPbL6,7D2H2 has an effect of accelerating a phagocytosis of RPE and increases the AGE degradation. In order to further understand effect of accelerating a phagocytosis of RPE, some further operations are performed. 5 [0051] DCMPbL6,7D2H2 is chromatographed by HPLC reverse C18 column (10 x 300 mm) with the mobile phase of methanol : H 2 0: acetic acid = 40:60:1, and the eluate named as DCMPbL6,7D2H2H3 is obtained. Then, the DCMPbL6,7D2H2H3 is further chromatographed by HPLC reverse C18 column (4.6 x 250 mm) with the mobile phase of methanol : H 2 O= 35:65, and 10 the eluate named as DCMPbL6,7D2H2H3H2 is obtained. Further, the DCMPbL6,7D2H2H3 is also chromatographed by HPLC reverse C18 column (4.6 x 250 mm) with the mobile phase of methanol : H 2 0= 40:60, and the eluate named as DCMPbL6,7D2H2H3H3 is obtained. Moreover, the DCMPbL6,7D2H2H3 is also chromatographed by HPLC reverse C18 column 15 (4.6 x 250 mm) with the mobile phase of methanol : H 2 0= 45:55, and the eluate named as DCMPbL6,7D2H2H3H4 is obtained. [0052] Please refer to Fig. 2, which is the LC-MS chromatography of DCMPbL6,7D2H2H3. The analysis conditions for Fig. 2 are: column: Mightysil RP-C18 (4.6*250mm), 5ptm; mobile phase: 0-80min 20 MeOH/H2O=20/80 gradient to 100% MeOH, 80~100min 100% MeOH; inject volume :5 0pl; inject weight : 5p[g; flow rate: 0.25 ml/min and UV: 337nm. [0053] Please refer to Fig. 3, which is the HPLC chromatography of DCMPbL6,7D2H2H3. The analysis conditions for Fig. 3 are: column: Mightysil RP-C18 (4.6*250mm), 5pm; mobile phase: 0-40min 25 MeOH/H2O=35/65, 40~45min MeOH/H20 = 35/65 gradient to 100% MeOH, 45~60min 100%MeOH; inject volume: 250pl; inject weight: 250ig; flow rate: 14 0.8 ml/min and UV: 280, 312, 337 nm. H2 represents DCMPbL6,7D2H2H3H2, H3 represents DCMPbL6,7D2H2H3H3, and H4 DCMPbL6,7D2H2H3H4. [00541 Please refer to Fig. 4, which is the LC-MS chromatography of 5 DCMPbL6,7D2H2H3H2. The analysis conditions for Fig. 4 are: column: Mightysil RP-C18 (4.6*250mm), 5pm; Mobile phase: 0-80min MeOH/H2O=20/80 gradient to 100%MeOH, 80~100min 100%MeOH; inject volume: 100ptl; inject weight: 5ptg; flow rate: 0.8 ml/min; UV: 220 nm and MS condition: APCI+ mode. 10 [0055] Please refer to Fig. 5, which is another LC-MS chromatography of DCMPbL6,7D2H2H3H2. The analysis conditions for Fig. 5 are: column: Mightysil RP-C18 (4.6*250mm), 5pim; mobile phase: 0~80min MeOH/H20 = 20/80 gradient to 100%MeOH, 80~100min 100%MeOH; inject volume: 100ptl; inject weight: 5ptg; flow rate: 0.8 ml/min; UV: 220 nm and MS condition: 15 APCI- mode. [0056] Please refer to Fig. 6, which is the LC-MS chromatography of DCMPbL6,7D2H2H3H3. The analysis conditions for Fig. 6 are: column: Mightysil RP-C18 (4.6*250mm), 5pm; mobile phase: 0~80min MeOH/H20 = 20/80 gradient to 100%MeOH, 80~100min 100%MeOH; inject volume: 100l; 20 inject weight: 5ptg; flow rate: 0.8 ml/min; UV: 220 nm and MS condition: APCI+ mode. [00571 Please refer to Fig. 7, which is the UV spectrum of the methanol solution of DCMPbL6,7D2H2H3H4 (50ptg/ml). As shown in Fig. 7, DCMPbL6,7D2H2H3H4 has characteristic bands: I at X= 334 nm, and band II 25 at k= 275 nm, which leads to the conclusion that the compound belongs to the group of flavonoides. 15 [0058] Based on the above mentioned, it is found that the molecular weight of DCMPbL6,7D2H2H3 is about 534 and it could be one selected from a group consisting of apigenin 6,8-Di-C-a-L-arabinopyranoside, apigenin 6- C- -D-Xylopyranosyl-8- C-a-L-arabinopyranoside, apigenin 6-C-a-L 5 Arabinopyranosyl-8-C- -D-arabinopyranoside and the mixture thereof. The structure of apigenin 6-C- -D-Xylopyranosyl-8-C-a-L-arabinopyranoside is shown in the following: HO HO 0 OH 60 90 0 o (Z) H OH O 60 90 OH27 M.w.= 534 [0059] In addition, the IR analyses of DCMPbL6,7D2H2H3 (not shown 10 herein) shows that the structural features of the DCMPbL6,7D2H2H3 coincide with those of the 6,8-Di-C-glycosides of apigenin. [0060] Furthermore, after being added some agents (AiCl 3 , NaOAc, NaOAc+H 3

BO

3 ) thereinto, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3, DCMPbL6,7D2H2H3H2 and DCMPbL6,7D2H2H3H4 are proceeded with the 15 UV analyses. The UV results show that the structural features of the 16 DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3, DCMPbL6,7D2H2H3H2 and DCMPbL6,7D2H2H3H4 coincide with those of the flavonoids (wherein there are hydroxyl groups at C-5 and C-7 positions, but there is no hydroxyl group at C-3 position). 5 [00611 Moreover, after being proceeded with NMR analyses, the H-NMR and C-NMR spectra of DCMPbL6,7D2H2H3, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3 and DCMPbL6,7D2H2H3H4 show that the relevant structural features thereof coincide with those of 6,8-Di-C-glycosides of apigenin. 10 [00621 As above, it is found the substances from DCMPbL6,7D2H2, i.e. DCMPbL6,7D2H2H3, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3 and DCMPbL6,7D2H2H3H4, could be 6,8-Di-C-glycosides of apigenin or the derivatives thereof, and they should have the structure as follows. R2 8 HO O R, 3 5 OH O 15 wherein Ri and R 2 are the same or different and are ones selected from a group consisting of ax-Ara, p-Ara and p-Xyl. The R 1 and R 2 could be identical or different single sugar moieties. [00631 However, since the natural C-glycosylflavonoids of C-glycosyl residues include C-p-D-glucopyranosyl, C-p-D-galactopyranosyl, 20 C-p-D-xylopyranosyl, C-p-D-arabinopyranosyl, C-a-L-arabinopyranosyl, C-ax-L-rhamnopyranosy land C-6-deoxy-xylo-hexos-4-ulosyl and the above 17 experiments have shown that DCMPbL6,7D2H2H3, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3 and DCMPbL6,7D2H2H3H4 have the above main structure of C-glycosides, it is believed that when the R 1 and R2 are also selected from the above 7 natural C-glycosylflavonoids, 48 relevant 5 compounds will be obtained therefrom. Similarly, if the R 1 and R2 are also selected from the unnatural C-glycosyl residues, 482 relevant compounds will be obtained therefrom. Accordingly, if the functional groups at C-4', C-5 and C-7 positions are substituted with other functional groups, the number of the relevant derivatives will be enormous. Table 1 shows the possible natural 10 functional group combinations of single sugar moiety for R1 and R 2 . Table 1. The possible natural functional group combinations for R 1 and R2. Compound R 1 R2 1* a-Ara a-Ara 2* p-Xyl a-Ara 3* a-Ara p-Xyl 4 p-Ara p-Ara 5 a-Ara p-Ara 6 p-Ara a-Ara 7 p-Xyl p-Ara 8 p-Ara p-Xyl 9 c-6-Deoxy-xylo-hexos-4-ul a-Ara osyl 10 c-6-Deoxy-xylo-hexos-4-ul p-Ara osyl 11 c-6-Deoxy-xylo-hexos-4-ul P-Xyl osyl 12 p-Xyl c-6-Deoxy-xylo-hexos-4-ul osyl 13 a-Ara c-6-Deoxy-xylo-hexos-4-ul osyl 14 6-Ara c-6-Deoxy-xylo-hexos-4-ul 18 osyl 15 c-6-Deoxy-xylo-hexos-4-ul c-6-Deoxy-xylo-hexos-4-ul osyl osyl 16 a-Ara p-Glu 17 a-Ara P-Gal 18 p-Ara p-Glu 19 P-Ara p-Gal 20 p-Glu cc-Ara 21 p-Gal C-Ara 22 p-Glu P-Ara 23 P-Gal p-Ara 24 P-Xyl p-Glu 25 P-Xyl p-Gal 26 P-Glu P-Xyl 27 p-Gal p-Xyl 28 c-6-Deoxy-xylo-hexos-4-ul p-Glu osyl 29 c-6-Deoxy-xylo-hexos-4-ul P-Gal osyl 30 P-Glu c-6-Deoxy-xylo-hexos-4-ul osyl 31 p-Gal c-6-Deoxy-xylo-hexos-4-ul osyl 32 a-Ara ax-Rha 33 p-Ara a-Rha 34 c-Rha a-Ara 35 a-Rha P-Ara 36 p-Xyl a-Rha 37 ax-Rha p-Xyl 38 c-6-Deoxy-xylo-hexos-4-ul ax-Rha osyl 39 cc-Rha c-6-Deoxy-xylo-hexos-4-ul osyl 40 p-Glu p-Glu 41 p-Glu p-Gal 42 p-Gal p-Glu 19 43 p-Gal p-Gal 44 p-Glu a-Rha 45 a-Rha p-Glu 46 p-Gal a-Rha 47 cc-Rha p-Gal 48 c-Rha a-Rha *: major products. [00641 Furthermore, the possible unnatural functional group compositions of single sugar moiety for R 1 and R 2 are shown in Table 2. 5 Table 2. The possible unnatural functional group compositions for R 1 and R 2 . Compound R 1 (or R 2 ) R 2 ( or R 1 ) 1 P-Ribose p-Ribose 2 p-Ribose a-Arabinose 3 P-Ribose P-Arabinose 4 P-Ribose p-Xylose 5 p-Ribose P-Lyxose 6 p-Ribose p-Allose 7 p-Ribose p-Altrose 8 p-Ribose p-Mannose 9 p-Ribose p-Gulose 10 p-Ribose p-Idose 11 p-Ribose p-Talose 12 p-Ribose p-Tagatose 13 P-Ribose P-Fructose 14 P-Ribose p-Glucose 15 P-Ribose p-Galactose 16 p-Ribose a-Rhamnose 17 p-Ribose c-6-Deoxy-xylo-hexos-4-ul osyl 18 a-Arabinose p-Ribose 19 a-Arabinose p-Lyxose 20 a-Arabinose p-Allose 21 a-Arabinose p-Altrose 20 22 c-Arabinose P-Mannose 23 ax-Arabinose P-Gulose 24 c-Arabinose P-Idose 25 c-Arabinose p-Talose 26 a-Arabinose p-Tagatose 27 ac-Arabinose 0-Fructose 28 p-Arabinose P-Ribose 29 p-Arabinose P-Lyxose 30 P-Arabinose P-Allose 31 P-Arabinose P-Altrose 32 P-Arabinose p-Mannose 33 p-Arabinose p-Gulose 34 P-Arabinose p-Idose 35 p-Arabinose P-Talose 36 p-Arabinose p-Tagatose 37 p-Arabinose p-Fructose 38 P-Xylose p-Ribose 39 p-Xylose p-Lyxose 40 p-Xylose P-Allose 41 P-Xylose P-Altrose 42 6-Xylose P-Mannose 43 p-Xylose P-Gulose 44 P-Xylose P-Idose 45 p-Xylose p-Talose 46 6-Xylose p-Tagatose 47 P-Xylose 0-Fructose 48 p-Lyxose p-Ribose 49 P-Lyxose a-Arabinose 50 P-Lyxose P-Arabinose 51 P-Lyxose p-Xylose 52 6-Lyxose p-Lyxose 53 P-Lyxose p-Allose 54 6-Lyxose p-Altrose 55 p-Lyxose p-Mannose 56 6-Lyxose p-Gulose 21 57 P-Lyxose p-Idose 58 p-Lyxose p-Talose 59 P-Lyxose P-Tagatose 60 p-Lyxose P-Fructose 61 P-Lyxose p-Glucose 62 p-Lyxose p-Galactose 63 p-Lyxose ax-Rhamnose 64 p-Lyxose c-6-Deoxy-xylo-hexos-4-ul osyl 65 p-Allose P-Ribose 66 p-Allose c-Arabinose 67 P-Allose p-Arabinose 68 p-Allose p-Xylose 69 P-Allose p-Lyxose 70 P-Allose p-Allose 71 p-Allose p-Altrose 72 6-Allose p-Mannose 73 p-Allose p-Gulose 74 p-Allose p-Idose 75 p-Allose p-Talose 76 p-Allose p-Tagatose 77 p-Allose P-Fructose 78 p-Allose p-Glucose 79 p-Allose p-Galactose 80 p-Allose c-Rhamnose 81 P-Allose c-6-Deoxy-xylo-hexos-4-ul osyl 82 P-Altrose P-Ribose 83 P-Altrose a-Arabinose 84 P-Altrose p-Arabinose 85 P-Altrose p-Xylose 86 p-Altrose p-Lyxose 87 p-Altrose p-Allose 88 p-Altrose p-Altrose 89 P-Altrose p-Mannose 22 90 p-Altrose p-Gulose 91 p-Altrose p-Idose 92 p-Altrose p-Talose 93 P-Altrose P-Tagatose 94 P-Altrose p-Fructose 95 p-Altrose P-Glucose 96 6-Altrose P-Galactose 97 P-Altrose a-Rhamnose 98 P-Altrose c-6-Deoxy-xylo-hexos-4-ul osyl 99 p-Mannose p-Ribose 100 P-Mannose c-Arabinose 101 P-Mannose P-Arabinose 102 p-Mannose p-Xylose 103 p-Mannose P-Lyxose 104 p-Mannose P-Allose 105 P-Mannose p-Altrose 106 p-Mannose p-Mannose 107 P-Mannose p-Gulose 108 p-Mannose P-Idose 109 p-Mannose P-Talose 110 P-Mannose p-Tagatose 111 p-Mannose 1-Fructose 112 p-Mannose p-Glucose 113 p-Mannose P-Galactose 114 P-Mannose a-Rhamnose 115 p-Mannose c-6-Deoxy-xylo-hexos-4-ul osyl 116 P-Gulose p-Ribose 117 p-Gulose a-Arabinose 118 p-Gulose p-Arabinose 119 p-Gulose P-Xylose 120 P-Gulose p-Lyxose 121 p-Gulose p-Allose 122 p-Gulose p-Altrose 23 123 p-Gulose p-Mannose 124 P-Gulose p-Gulose 125 P-Gulose p-Idose 126 P-Gulose p-Talose 127 p-Gulose p-Tagatose 128 P-Gulose p-Fructose 129 P-Gulose p-Glucose 130 p-Gulose p-Galactose 131 P-Gulose a-Rhamnose 132 P-Gulose c-6-Deoxy-xylo-hexos-4-ul osyl 133 p-Idose p-Ribose 134 p-Idose c-Arabinose 135 p-Idose p-Arabinose 136 p-Idose p-Xylose 137 p-Idose p-Lyxose 138 p-Idose p-Allose 139 P-Idose p-Altrose 140 p-Idose P-Mannose 141 p-Idose p-Gulose 142 p-Idose p-Idose 143 p-Idose p-Talose 144 p-Idose p-Tagatose 145 P-Idose p-Fructose 146 p-Idose P-Glucose 147 p-Idose p-Galactose 148 p-Idose ca-Rhamnose 149 p-Idose c-6-Deoxy-xylo-hexos-4-ul osyl 150 p-Talose P-Ribose 151 p-Talose ax-Arabinose 152 P-Talose p-Arabinose 153 p-Talose P-Xylose 154 p-Talose p-Lyxose 155 P-Talose p-Allose 24 156 p-Talose p-Altrose 157 P-Talose P-Mannose 158 p-Talose p-Gulose 159 P-Talose p-Idose 160 p-Talose p-Talose 161 p-Talose P-Tagatose 162 p-Talose P-Fructose 163 p-Talose p-Glucose 164 P-Talose 1-Galactose 165 P-Talose a-Rhamnose 166 p-Talose c-6-Deoxy-xylo-hexos-4-ul osyl 167 P-Tagatose P-Ribose 168 P-Tagatose a-Arabinose 169 P-Tagatose P-Arabinose 170 P-Tagatose p-Xylose 171 p-Tagatose p-Lyxose 172 p-Tagatose p-Allose 173 p-Tagatose p-Altrose 174 p-Tagatose P-Mannose 175 P-Tagatose P-Gulose 176 p-Tagatose P-Idose 177 p-Tagatose p-Talose 178 p-Tagatose P-Tagatose 179 p-Tagatose p-Fructose 180 P-Tagatose p-Glucose 181 p-Tagatose p-Galactose 182 P-Tagatose a-Rhamnose 183 p-Tagatose c-6-Deoxy-xylo-hexos-4-ul osyl 184 1-Fructose p-Ribose 185 p-Fructose a-Arabinose 186 P-Fructose p-Arabinose 187 P-Fructose p-Xylose 188 p-Fructose p-Lyxose 25 189 p-Fructose p-Allose 190 p-Fructose p-Altrose 191 p-Fructose p-Mannose 192 p-Fructose p-Gulose 193 p-Fructose P-Idose 194 p-Fructose B-Talose 195 p-Fructose P-Tagatose 196 p-Fructose p-Fructose 197 p-Fructose 1-Glucose 198 P-Fructose p-Galactose 199 P-Fructose a-Rhamnose 200 P-Fructose c-6-Deoxy-xylo-hexos-4-ul osyl 201 p-Glucose p-Ribose 202 1-Glucose p-Lyxose 203 p-Glucose p-Allose 204 p-Glucose p-Altrose 205 p-Glucose 1-Mannose 206 p-Glucose p-Gulose 207 p-Glucose p-Idose 208 p-Glucose p-Talose 209 p-Glucose p-Tagatose 210 p-Glucose P-Fructose 211 p-Glucose c-6-Deoxy-xylo-hexos-4-ul osyl 212 P-Galactose p-Ribose 213 p-Galactose p-Lyxose 214 p-Galactose p-Allose 215 p-Galactose p-Altrose 216 P-Galactose p-Mannose 217 p-Galactose p-Gulose 218 p-Galactose 1-Idose 219 p-Galactose 1-Talose 220 p-Galactose p-Tagatose 221 p-Galactose 1-Fructose 26 222 a-Rhamnose p-Ribose 223 ax-Rhamnose p-Lyxose 224 a-Rhamnose p-Allose 225 a-Rhamnose P-Altrose 226 c-Rhamnose P-Mannose 227 ax-Rhamnose p-Gulose 228 a-Rhamnose p-Idose 229 a-Rhamnose p-Talose 230 a-Rhamnose p-Tagatose 231 a-Rhamnose p-Fructose 232 c-6-Deoxy-xylo-hexos-4-ul P-Ribose osyl 233 c-6-Deoxy-xylo-hexos-4-ul P-Lyxose osyl 234 c-6-Deoxy-xylo-hexos-4-ul p-Allose osyl 235 c-6-Deoxy-xylo-hexos-4-ul p-Altrose osyl 236 c-6-Deoxy-xylo-hexos-4-ul P-Mannose osyl 237 c-6-Deoxy-xylo-hexos-4-ul P-Gulose osyl 238 c-6-Deoxy-xylo-hexos-4-ul p-Idose osyl 239 c-6-Deoxy-xylo-hexos-4-ul P-Talose osyl 240 c-6-Deoxy-xylo-hexos-4-ul P-Tagatose osyl 241 c-6-Deoxy-xylo-hexos-4-ul p-Fructose osyl [0065] Please refer to Fig. 8, which is the bar chart illustrating the effects of DCMPbL6,7D2H2H3 on the total phagocytosis of RPE. As shown in Fig. 8, it's known that various concentrations (0.1, 1, 10, 100 pig/ml) of 5 DCMPbL6,7D2H2H3 can accelerate the phagocytosis of RPE. The relevant 27 experimental contents are simply described as follows. 1x104 RPE cells are seeded in 96-well microplate per well, containing 5% FCS in DMEM after 48hrs, and the medium is changed with 2% FCS in DMEM. Then different concentrations of DCMPbL6,7D2H2H3 are added respectively. After 24 hrs, 5 50 ptl of 2x 107 FITC-ROS/ml is added into each well. Four hours later, the unbounded FITC-ROS is washed out with PBS+2%sucrose. The emission wavelength is 485nm and the detecting wavelength is 530nm. The fluorescence intensity is detected by a 1420 Multilable counter (PE) measurement system. The detected fluorescence intensity represents the total 10 phagocytosis. If the fluro Quench dye is added, the detected fluorescence intensity represents the ingestion phagocytosis. *P< 0.05, ** P <0.02, ***P<0.01 and ****P<0.001 are obtained by comparing with phagocytosis of RPE treated with 2% FCS. In addition, Fig. 9 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3 on the ingestion phagocytosis of RPE. 15 [0066] Please refer to Fig. 10, which is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H2 on the total phagocytosis of RPE. As shown in Fig. 10, it's known that various concentrations (0.1, 1, 10, 100 pg/ml) of DCMPbL6,7D2H2H3H2 can accelerate the phagocytosis of RPE. The relevant experimental contents are simply described as follows. 1x104 RPE 20 cells are seeded in 96-well microplate per well, containing 5% FCS in DMEM after 48hrs, and the medium is changed with 2% FCS in DMEM. Then different concentrations of DCMPbL6,7D2H2H3H2 are added respectively. After 24 hrs, 50 pl of 2x107 FITC-ROS/ml is added into each well. Four hours later, the unbounded FITC-ROS is washed out with PBS+2%sucrose. 25 The emission wavelength is 485nm and the detecting wavelength is 530nm. The fluorescence intensity is detected by a 1420 Multilable counter (PE) 28 measurement system. The detected fluorescence intensity represents the total phagocytosis. If the fluro Quench dye is added, the detected fluorescence intensity represents the ingestion phagocytosis. *P< 0.05, ** P <0.02, ***P<0.01 and ****P<0.001 are obtained by comparing with phagocytosis of 5 RPE treated with 2% FCS. In addition, Fig. 11 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H2 on the ingestion phagocytosis of RPE. [0067] Please refer to Fig. 12, which is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H3 on the total phagocytosis of RPE. As shown in Fig. 12, it's known that various concentrations (0.1, 1, 10, 100 gg/ml) 10 of DCMPbL6,7D2H2H3H3 can accelerate the phagocytosis of RPE. The relevant experimental contents are simply described as follows. 1x104 RPE cells are seeded in 96-well microplate per well, containing 5% FCS in DMEM after 48hrs, and the medium is changed with 2% FCS in DMEM. Then different concentrations of DCMPbL6,7D2H2H3H3 are added respectively. 15 After 24 hrs, 50 pl of 2x107 FITC-ROS/ml is added into each well. Four hours later, the unbounded FITC-ROS is washed out with PBS+2%sucrose. The emission wavelength is 485nm and the detecting wavelength is 530nm. The fluorescence intensity is detected by a 1420 Multilable counter (PE) measurement system. The detected fluorescence intensity represents the total 20 phagocytosis. If the fluro Quench dye is added, the detected fluorescence intensity represents the ingestion phagocytosis. *P< 0.05, ** P <0.02, ***P<0.01 and ****P<0.001 are obtained by comparing with phagocytosis of RPE treated with 2% FCS. In addition, Fig. 13 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H3 on the ingestion phagocytosis of RPE. 25 [0068] Please refer to Fig. 14, which is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H4 on the total phagocytosis of RPE. As 29 shown in Fig. 14, it's known that various concentrations (0.1, 1, 10, 100 pig/ml) of DCMPbL6,7D2H2H3H4 can accelerate the phagocytosis of RPE. The relevant experimental contents are simply described as follows. 1x104 RPE cells are seeded in 96-well microplate per well, containing 5% FCS in DMEM 5 after 48hrs, and the medium is changed with 2% FCS in DMEM. Then different concentrations of DCMPbL6,7D2H2H3H4 are added respectively. After 24 hrs, 50 pl of 2x107 FITC-ROS/ml is added into each well. Four hours later, the unbounded FITC-ROS is washed out with PBS+2%sucrose. The emission wavelength is 485nm and the detecting wavelength is 530nm. 10 The fluorescence intensity is detected by a 1420 Multilable counter (PE) measurement system. The detected fluorescence intensity represents the total phagocytosis. If the fluro Quench dye is added, the detected fluorescence intensity represents the ingestion phagocytosis. *P< 0.05, ** P <0.02, ***P<0.01 and ****P<0.001 are obtained by comparing with phagocytosis of 15 RPE treated with 2% FCS. In addition, Fig. 15 is the bar chart illustrating the effects of DCMPbL6,7D2H2H3H4 on the ingestion phagocytosis of RPE. [0069] Based on Figs. 2 - 7, it seems that DCMPbL6,7D2H2H3, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3 and DCMPbL6,7D2H2H3H4, could be 6,8-Di-C-glycosides of apigenin or the 20 derivatives thereof, and they should have the structure as follows. OH R2 4 8 HO O R 3 5 OH 3 30 wherein R 1 and R2 are the same or different and are ones selected from Table 1 and Table 2. [0070] Furthermore, based on Figs. 8 - 15, it is known that DCMPbL6,7D2H2H3, DCMPbL6,7D2H2H3H2, DCMPbL6,7D2H2H3H3 5 and DCMPbL6,7D2H2H3H4 could accelerate the phagocytosis of RPE. As above mentioned, it is realized that the substance with the mentioned structure would accelerate the phagocytosis of RPE. [0071] Furthermore, based on the prior references, it is realized that a composition including the substance capable of accelerating the phagocytosis 10 of RPE would have effects on at least one selected from the groups consisting of RPE degeneration, age-related macular degeneration, photoreceptor degeneration, diabetes, arteriosclerosis, vascular damage, retinal neuropathy, amacrine cell dysfunction, retinal ganglion cells degeneration, Mnller cell dysfunction, uveitis, choroidal neovascularization, proliferative retinopathy, 15 retinitis, Bruch's membrane disorder and a complication thereof. The mentioned disorders could be generalized as RPE cell-related disorders. [0072] While the invention has been described in terms of what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention needs not be limited to the disclosed 20 embodiment. On the contrary, it is intended to cover various modifications and similar arrangements included within the spirit and scope of the appended claims which are to be accorded with the broadest interpretation so as to encompass all such modifications and similar structures. 31

Claims (7)

1. Use in manufacturing a medicament for treating a retinal pigment epithelium (RPE) related disorder, wherein the medicament comprises a substance selected from the group consisting of apigenin 6 , 8 -Di-C-c-L-arabinopyranoside, apigenin 6 -C--D-Xylopyranosyl-8-C-a-L-arabinopyranoside, and apigenin 6 -C-a-L-Arabinopyranosyl-8-C-p-D-arabinopyranoside.

2. The use of claim 1, wherein the substance is isolated from Herba Dendrobii.

3. The use of claim 1, wherein the phagocytosis of RPE is enhanced by the substance.

4. A pharmaceutical composition when used to treat and/or prevent a retinal pigment epithelium (RPE) related disorder, comprising-a physiologically acceptable carrier and a substance, wherein the substance is selected from the group consisting of apigenin 6,8-Di-C-a-L-arabinopyranoside, apigenin 6 -C-P-D-Xylopyranosyl-8-C-a-L-arabinopyranoside, and apigenin 6 -C-a-L-Arabinopyranosyl-g-C-P-D-arabinopyranoside.

5. The pharmaceutical composition of claim 4, wherein the RPE related disorder is selected from the group consisting of RPE degeneration, age-related macular degeneration, photoreceptor degeneration, diabetes, arteriosclerosis, vascular damage, retinal neuropathy, amacrine cell dysfunction, retinal ganglion cells degeneration, Muller cell dysfunction, uveitis, choroidal neovascularization, proliferative retinopathy, retinitis, Bruch's membrane disorder and a complication thereof.

6. The pharmaceutical composition of claim 4, wherein the substance is 32 isolated from Herba Dendrobii.

7. The pharmaceutical composition of claim 4, comprising the substance in an amount effective for enhancing the function of RPE. 33