AU2003221640A1

AU2003221640A1 - Anti-tumour polycyclic carboxamides

Info

Publication number: AU2003221640A1
Application number: AU2003221640A
Authority: AU
Inventors: Bruce Charles Baguley; Leslie William Deady; William Alexander Denny; Thomas Rodemann; Michael Leslie Rogers
Original assignee: La Trobe University
Current assignee: Auckland Uniservices Ltd
Priority date: 2002-05-15
Filing date: 2003-05-12
Publication date: 2003-12-02
Anticipated expiration: 2023-05-12
Also published as: AU2003221640B2

Description

WO 03/097642 PCT/AU03/00569 - 1 ANTI-TUMOUR POLYCYCLIC CARBOXAMIDES This invention relates to a novel class of polycyclic carboxamide compounds with cytotoxicity, 5 processes for their preparation, pharmaceutical compositions containing them and their use in therapy, particularly in the treatment and/or prophylaxis of cellular proliferative disorders such as cancer. 10 BACKGROUND OF THE INVENTION Compounds comprising polycyclic chromophores bearing a flexible cationic side chain have been known to show cytotoxic effects and to possess utility as anticancer drugs. 15 Tricyclic chromophores that show cytotoxic activity include benzoisoquinolinediones (e.g., amonafide, 1; Asbury et al., 1998; Leaf et al., 1997), acridine-4 carboxamides (e.g., DACA, 2; Atwell et al., 1987; Baguley et al., 1995; U.S. Patent No. 4,590,277) and anthraquinones 20 (e.g., mitoxantrone, 3; Koller et al., 1999). Tetracyclic chromophores with cytotoxic activity include anthrapyrazoles (e.g., losoxantrone, 4; Diab et al., 1999; Judson, 1992), indeno[2,l-c]quinolin-7-ones (e.g., TAS-103, 5; Utsugi et al., 1996), benzophenazines 25 (e.g., XR-11576, 6; Vicker et al., 2002), azonafides (e.g. 7; Sami et al., 1996), imidazoacridinones (e.g. 8; Cholody et al., 1996), pyrimido[5,6,l-de]acridines (e.g. 9; Antonini et al., 1995), benzo[e]pyrido[4,3-b]indoles (e.g., intoplicine, 10; Riou et al., 1993), indeno[l,2 30 b]quinolines (e.g. 11, Deady et al., 1997) and benzo[e]perimidines (e.g. 12, Stefanska et al., 1993). Many compounds where two such chromophores are linked by a flexible chain are also effective cytotoxins and anticancer drugs. For example, the bis(naphthalimide) 35 DMP840, 13, was evaluated clinically (Thompson et al., 1998), bis(imidazoacridones) (e.g., WMC-26, 14; Cholody et al. 1995) and bis(phenazines) (e.g., XR5944, 15; Gamage et WO 03/097642 PCT/AU03/00569 -2 al., 2001; Stewart et al., 2001) are being considered for clinical trial. From the publications referred to above, it can be seen that these compounds have a wide variety of 5 different structures. They are considered to be active as anticancer drugs primarily through their ability to inhibit topoisomerase enzymes. However, the relationships between structure, the ability to inhibit topoisomerase enzymes and their potential utility as in vivo anticancer 10 drugs are still not sufficiently well-defined to enable predictions to be made about activity. In view of the potential utility of such compounds, further classes of these compounds are needed. The present invention relates to amide- (and 15 thioamide) derivatives of benzo[b][1,6]naphthyridin-l(2H) one, their synthesis, and their use in the treatment of cancers. The benzo[b] [l,6]naphthyridin-l(2H)-one chromophore (Deady and Rodemann, 2001) and the N2- and carbon-substituted analogues (Asherson and Young, 1977; 20 Khattab, 1996; Meth-Cohn, 1987; Rivalle and Bisagni, 1980) are known including the 6-methyl-l-oxo-l,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (Deady and Rodemann, 2001). Dibenzo[b,h][1,6]naphthyridin-6(5H) ones have also been reported (Asherson and Young, 1977; 25 Vijayalakshmi and Rajendran, 1994). However, these reports concern the synthesis of the compounds only. It will be clearly understood that, although a number of prior art publications are referred to herein, this reference does not constitute an admission that any 30 of these documents forms part of the common general knowledge in the art, in Australia or in any other country. We have now synthesised and evaluated a novel series of carboxamide linked compounds based on 35 benzo[b] [l,6]naphthyridin-l(2H)-one, which differ from previous acridine based compounds by the incorporation of a lactam or thiolactam function in one of the outer rings.

WO 03/097642 PCT/AU03/00569 - 3 Representative examples of these compounds have utility as cytotoxic and anti-cancer agents. SUMMARY OF THE INVENTION 5 According to a first aspect, the invention provides a compound of the formula I Q 9 10 QI1 zN2 10 N 6 5 4 W I in which positional numbering-, where mentioned, 15 refers to the system illustrated above, and one or more W and one or more Z are attached to a ring carbon or carbons at any of positions 3,4 and 6 to 10, and in which: Q is O or S; W is C(=Q)NR-M-(CH 2 )mR 1 , in which 20 M is CHJ or G, R is H or an optionally substituted C 1

-

4 alkyl group, J is H or an optionally substituted CI-C 6 alkyl group, 25 G is an optionally substituted fully saturated, or partially unsaturated, or aromatic, carbocycle or heterocycle, R is C(=NR 2

)NH

2 , NHC(=NR 3

)NH

2 or NR 4

R

5 , in which each of R 2 and R 3 are independently H or an 30 optionally substituted C 1 4 alkyl group,

R

4 and R 5 are independently H or an optionally substituted C 1

-..

4 alkyl group or R 4 and R 5 together with the nitrogen atom to which they are attached form an optionally substituted, saturated or unsaturated 35 heterocyclic group, and m is an integer from 0 to 6; Y is H, C 1

-

6 alkyl or (CH 2 )n-X-(CH 2 )pU, in which WO 03/097642 PCT/AU03/00569 - 4 X is CH 2 , C=O, CH=CH, O, S, NR or G; and n and p are integers from 0 to 6, and U is H, CF 3 , halo, NR 4

R

5 , +NRR 4

R

5 , cyano,

C(=O)NRR

5 , OR 4 , C0 2

R

4 , G, NR 4 G or OG; and 4 4 45 5 Z is H, halo, OH, CO 2 H, CO 2 R , SO 2 R , NR R', nitro, cyano, C 1 -6 alkyl, Ci-6 haloalkyl, C 1 -6 alkoxy, Ci-6 haloalkoxy, C 1 -6 aminoalkyl, C 1 -6 aminoalkoxy, or aza functionality replacing a ring CH functionality, or a carbon or carbon/nitrogen framework bridging the 6-7, 7-8 10 or 8-9 positions so as to form an additional fused 5 to 6 membered carbocycle or heterocycle; or a pharmaceutically-acceptable salt, N-oxide, hydrate, solvate, pharmaceutically acceptable derivative, pro-drug, tautomer and/or isomer thereof. 15 The optional substituents for R, R 2 , R 3 , R and R 5 are preferably one or more OH or NH 2 groups and for J are one or more OH, OMe, NH 2 , NHMe or NMe 2 groups. The preferred optional substituents for G are one or more halo, OH, CO 2 H, NR 4

R

5 , NRSO 2 R, SO 2 NR R 5 , nitro, cyano, C 1 -6 20 alkyl, CI-6 haloalkyl, C 1 -6 alkoxy, CI-6 haloalkoxy, CI-6 aminoalkyl, C1-6 aminoalkoxy or B(OR6) (OR 7 ) in which R 6 and

R

7 are independently hydrogen or an optionally substituted

C

1

-

4 alkyl group or together with the O and B atoms to which they are attached form an optionally substituted, 25 fully saturated, or partially unsaturated, heterocycle. Preferably Q is O; W is CONH(CH 2

)

2

N(CH

3

)

2 or

CONHCH(CH

3

)CH

2

N(CH

3

)

2 ; Y is methyl, butyl or methoxy substituted phenyl; Z is H, Cl, methyl or methoxy. A particularly preferred compound is N-[2 30 (dimethylamino)ethyl]-2,6-dimethyl-l-oxo-l,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide or a pharmaceutically acceptable salt or N-oxide thereof.

WO 03/097642 PCT/AU03/00569 - 5 The present invention also provides a compound of the formula II or III 9 10 5 8N--- -L z 7 N3 6 5 4 W 6 x - II 10 Q 10 9 10 8INY 8 1 yN 8 N3 7 N 6 5 4 CN(R) --- L 6 II x 15 - Q III in which one or more W and one or more Z in formula II, and one or more Z and C(=Q)NR in formula III 20 are attached to a ring carbon or carbons at any of positions 3, 4 and 6 to 10 and in which: Q, R, W, Y and Z are as defined in formula I; x is an integer from 2 to 4; and L is a linker group of valency x; or 25 a pharmaceutically-acceptable salt, N-oxide, hydrate, solvate, pharmaceutically acceptable derivative, pro-drug, tautomer and/or isomer thereof. For the avoidance of any doubt, it is to be understood that each monomer subunit of the compound may 30 be different, provided that in each subunit the substituents are individually within the definitions provided. In other words, compounds with two or more different units of formula I linked together are to be considered to be within the definition of formula II 35 above. Preferably x is 2. Preferably L is a nitrogen-containing linker WO 03/097642 PCT/AU03/00569 - 6 group, more preferably V 1- [ (CHR) qNR 4 ] r (CHR)q-V 2 in which V and V 2 are each independently oxygen, NR 4 or CHR; q is an integer from 0 to 5 and may have a 5 different value for each subunit of the linker L; r is an integer from 0 to 2;

R

4 is as defined in formula I, or when r is greater than 0, R and R 4 may together form an optionally substituted branched or straight chained alkylene. 10 Preferably R and R 4 are each independently H,

CH

3 , or C 2

H

5 , or if r is greater than 0, R and R 4 are preferably -CH 2

CH

2

CH

2 -. Most preferably the linker group will be selected from the following: 15 -(CH 2

)

2

NH(CH

2

)

2 -(CH 2

)

3 -NMe-(CH 2 )3

-(CH

2

)

2

NH(CH

2

)

2

NH(CH

2 )2 -(CH 2

)

2

NH(CH

2

)

3

NH(CH

2 )2

-(CH

2

)

2

N

M e

(CH

2

)

2

N

M e

(C

H

2

)

2 20 -(CH 2

)

2

N

M

e(CH 2

)

3 NMe(CH 2

)

2 -N,N'-Bis(ethylene)piperazine -N,N'-Bis(propylene)piperazine-,

-(CH

2 )sNH(CH 2 )t

-(CH

2 )sNAlkyl(CH 2 )t 25 -(CH 2 )sNH(CH 2 )tNH(CH 2 )u

-

, and

-(CH

2 )sNAlkyl(CH 2 )tNAlkyl(CH2)u-, in which s, t and u are integers from 2 to 6. According to a second aspect, the invention provides a process for the preparation of a compound of 30 formula I, II or III which comprises converting a compound of formula IV 9 10 Q N2 35 7 N 3 6 5 4 CO2H

IV

WO 03/097642 PCT/AU03/00569 - 7 in which one or more Z and one or more CO 2 H groups are attached to a ring carbon or carbons at any of positions 3, 4 and 6 to 10; and 5 Q, Y and Z are as defined in formula I into the amide of formula I, II or III. As the intermediate compound of formula IV is novel, the present invention also extends to compounds of formula IV as defined above. 10 According to a third aspect, the invention provides a process for the preparation of a compound of formula IV in which Q is 0 and CO 2 H is attached to position 4 comprising reacting a compound of formula V, O 9 10 1 15 8 0y .

2 Z 3 7 0 4 6 5 CHNMe 2 V 20 in which Z is as defined in formula I, with YNH 2 in which Y is as defined in formula I. In a fourth aspect, the invention provides a pharmaceutical or veterinary composition comprising the 25 compound of formula I, II or III as defined above, together with a pharmaceutically or veterinarily acceptable carrier. In a fifth aspect, the invention provides a method of treatment and/or prophylaxis of a cellular 30 proliferative disorder comprising administering a therapeutically effective amount of the compound of formula I, II or III to a subject in need thereof. The present invention also provides use of the compound of formula I, II or III in the manufacture of a 35 medicament for the treatment and/or prophylaxis of a cellular proliferative disorder. The present invention further provides the WO 03/097642 PCT/AU03/00569 - 8 compound of formula I, II or III for use in the treatment and/or prophylaxis of a cellular proliferative disorder. The present invention still further provides use of a compound of formula I, II or III as a cytotoxic, 5 anti-neoplastic, anti-tumour and/or anti-cancer agent. DETAILED DESCRIPTION OF THE INVENTION For the purposes of this specification it will be clearly understood that the word "comprising" means 10 "including but not limited to", and that the word "comprises" has a corresponding meaning. It must be noted that, as used in the subject specification, the singular forms "a", "an" and "the" include plural aspects unless the context clearly dictates 15 otherwise. Thus, for example, reference to "a compound of formula I, II or III" includes a single compound, as well as two or more compounds; and so forth. The terms "C 1

-

4 alkyl" or "CI- ~ alkyl" used either alone or in compound words such as "optionally substituted 20 C 1

-

4 or C1-6 alkyl", "Cl- 6 haloalkyl" or "Cl-6 aminoalkyl" refer to straight chain, branched chain or cyclic hydrocarbon groups having from 1 to 6 carbon atoms. Illustrative of such alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, 25 pentyl, neopentyl, hexyl, cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl. The term "heterocyclic group" used either alone or in compound words such as "optionally substituted saturated or unsaturated heterocyclic group" refers to 30 monocyclic or polycyclic heterocyclic groups containing at least one heteroatom atom selected from nitrogen, sulphur and oxygen. Suitable heterocyclic groups include N containing heterocyclic groups, such as, unsaturated 3 to 35 6-membered heteromonocyclic groups containing 1 to 4 nitrogen atoms, for example, pyrrolyl, pyrrolinyl, imidazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, WO 03/097642 PCT/AU03/00569 - 9 pyridazinyl, triazolyl or tetrazolyl; saturated 3 to 6-membered heteromonocyclic groups containing 1 to 4 nitrogen atoms, such as, pyrrolidinyl, imidazolidinyl, piperidino or piperazinyl; 5 unsaturated condensed heterocyclic groups containing 1 to 5 nitrogen atoms, such as indolyl, isoindolyl, indolizinyl, benzimidazolyl, quinolyl, isoquinolyl, indazolyl, benzotriazolyl or tetrazolopyridazinyl; 10 unsaturated 3 to 6-membered heteromonocyclic group containing an oxygen atom, such as, pyranyl or furyl; unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulphur atoms, such as, thienyl; 15 unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms, such as, oxazolyl, isoxazolyl or oxadiazolyl; saturated 3 to 6-membered heteromonocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms, 20 such as, morpholinyl; unsaturated condensed heterocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms, such as, benzoxazolyl or benzoxadiazolyl; unsaturated 3 to 6-membered heteromonocyclic 25 group containing 1 to 2 sulphur atoms and 1 to 3 nitrogen atoms, such as, thiazolyl or thiadiazolyl; saturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulphur atoms and 1 to 3 nitrogen atoms, such as, thiazolidinyl; and 30 unsaturated condensed heterocyclic group containing 1 to 2 sulphur atoms and 1 to 3 nitrogen atoms, such as, benzothiazolyl or benzothiadiazolyl. The term "optionally substituted" refers to a group may or may not be further substituted with one or 35 more groups selected from alkyl, alkenyl, alkynyl, aryl, halo, haloalkyl, haloalkenyl, haloalkynyl, haloaryl, hydroxy, alkoxy, alkenyloxy, aryloxy, benzyloxy, WO 03/097642 PCT/AU03/00569 - 10 haloalkoxy, haloalkenyloxy, haloaryloxy, nitro, nitroalkyl, nitroalkenyl, nitroalkynyl, nitroaryl, nitroheterocyclyl, amino, alkylamino, dialkylamino, alkenylamino, alkynylamino, arylamino, diarylamino, 5 benzylamino, dibenzylamino, acyl, alkenylacyl, alkynylacyl, arylacyl, acylamino, diacylamino, acyloxy, alkylsulphonyloxy, arylsulphenyloxy, heterocyclyl, heterocycloxy, heterocyclamino, haloheterocyclyl, alkylsulphenyl, arylsulphenyl, carboalkoxy, carboaryloxy, 10 mercapto, alkylthio, benzylthio, acylthio, phosphorus containing groups and the like. In some instances in this specification, where substituents may be present, preferred substituents have been mentioned. The term "carbocycle" refers to a fully 15 saturated, partially unsaturated or aromatic carbocyclic radical having 3 to 12 carbon atoms in a single ring or multiple condensed rings such as cycloalkyl, cycloalkenyl and aryl. Examples of cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Examples of 20 cycloalkenyl include cyclobutenyl, cyclopentenyl, cyclopentadienyl and cyclohexenyl. Examples of aryl include phenyl, naphthyl, tetrahydronaphthyl, indane and biphenyl. The term "halo" or "halogen" refers to fluorine, 25 chlorine, bromine, or iodine. Where halogen substitution is present, preferred halogens are chlorine or bromine. The term "C 1 -6 alkoxy" used either alone or in compound words such as "C-6 haloalkoxy" or "C 1 -6 aminoalkoxy" refers to straight chain or branched oxy 30 containing radicals each having alkyl portions of 1 to about 6 carbon atoms. Examples of alkoxy include methoxy, ethoxy, propoxy, butoxy and tert-butoxy. The term "linker group" is used herein in its broadest sense to refer to any organic group that links 35 together the adjacent units of the compound together and may be symmetrical or non-symmetrical. While the linker group is preferably a nitrogen-containing linker group, it WO 03/097642 PCT/AU03/00569 - 11 will be appreciated that it could alternatively be O; S; an optionally substituted C1- 20 alkylene, alkenylene or alkynylene chain which may optionally be interspersed with one or more optionally substituted aryl or optionally 5 substituted heterocyclic groups and/or one or more O, S or N atoms; or an optionally substituted saturated or unsaturated aryl or heterocyclic group. The terms "alkylene", "alkenylene" and "alkynylene" are the divalent radical equivalents of the 10 terms "alkyl", "alkenyl" and "alkynyl", respectively. The two bonds connecting the alkylene, alkenylene or alkynylene to the adjacent groups may come from the same carbon atom or different carbon atoms in the divalent radical. 15 The linker may alternatively be of the type disclosed in International Patent Application No. WO 96/25400 by The Du Pont Merck Pharmaceutical Company, the entire disclosure of which is incorporated by this cross reference. 20 The term "alkenyl" refers to linear or branched radicals having at least carbon-carbon double bond of 2 to 20 carbon atoms or, preferably 2 to 12 carbon atoms. More preferred alkenyl radicals are "C2-6 alkenyl". Examples of alkenyl include ethenyl, propenyl, allyl, propenyl, 25 butenyl and 4-methylbutenyl. The term "alkynyl" refers to linear or branched radicals having 2 to 20 carbon atoms, preferably 2 to 12 carbon atoms. More preferred alkynyl radicals are "C 2 -6 alkynyl". Examples of alkynyl include propargyl and 30 butynyl. The conversion step in the process for the preparation of the compound of formula I, II or III may involve an intermediate step in which the compound of formula IV is converted into an imidazolide and then 35 reacted with the appropriate amine to obtain the target amide of formula I, II or III. Alternatively, the reaction involves converting the carboxylic acid of WO 03/097642 PCT/AU03/00569 - 12 formula IV into an acid halide, followed by reaction with an amine to obtain the target amide of formula I, II or III. The reagent in this second route is preferably thionyl chloride. It will be clearly understood in the 5 above description that in the case of the bis compounds, two units of the carboxylic acid will be reacted with the appropriate diamine to form the target diamide. The salts of the compound of Formula I, II or III are preferably pharmaceutically acceptable, but it 10 will be appreciated that non-pharmaceutically acceptable salts also fall within the scope of the present invention, since these are useful as intermediates in the preparation of pharmaceutically acceptable salts. Examples of pharmaceutically acceptable salts include salts of 15 pharmaceutically acceptable cations such as sodium, potassium, lithium, calcium, magnesium, ammonium and alkylammonium; acid addition salts of pharmaceutically acceptable inorganic acids such as hydrochloric, orthophosphoric, sulphuric, phosphoric, nitric, carbonic, 20 boric, sulfamic and hydrobromic acids; or salts of pharmaceutically acceptable organic acids such as acetic, propionic, butyric, tartaric, maleic, hydroxymaleic, fumaric, citric, lactic, mucic, gluconic, benzoic, succinic, oxalic, phenylacetic, methanesulphonic, 25 trihalomethanesulphonic, toluenesulphonic, benzenesulphonic, salicylic, sulphanilic, aspartic, glutamic, edetic, stearic, palmitic, oleic, lauric, pantothenic, tannic, ascorbic and valeric acids. In addition, some of the compounds of the 30 present invention may form solvates with water or common organic solvents. Such solvates are encompassed within the scope of the invention. By "pharmaceutically acceptable derivative" is meant any pharmaceutically acceptable salt, hydrate, 35 ester, amide, active metabolite, analogue, residue or any other compound which is not biologically or otherwise undesirable and induces the desired pharmacological and/or WO 03/097642 PCT/AU03/00569 - 13 physiological effect. The term "pro-drug" is used herein in its broadest sense to include those compounds which are converted in vivo to compounds of Formula I, II or III. 5 The term "tautomer" is used herein in its broadest sense to include compounds of Formula I, II or III which are capable of existing in a state of equilibrium between two isomeric forms. Such compounds may differ in the bond connecting two atoms or groups and 10 the position of these atoms or groups in the compound. The term "isomer" is used herein in its broadest sense and includes structural, geometric and stereo isomers. As the compound of Formula I, II or III may have one or more chiral centres, it is capable of existing in 15 enantiomeric forms. The compositions of the present invention comprise at least one compound of Formula I, II or III together with one or more pharmaceutically acceptable carriers and optionally other therapeutic agents. Each 20 carrier, diluent, adjuvant and/or excipient must be pharmaceutically "acceptable" in the sense of being compatible with the other ingredients of the composition and not injurious to the subject. Compositions include those suitable for oral, rectal, nasal, topical (including 25 buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration. The compositions may conveniently be presented in unit dosage form and may be prepared by methods well known in the art of pharmacy. Such methods 30 include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients. In general, the compositions are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers, 35 diluents, adjuvants and/or excipients or finely divided solid carriers or both, and then if necessary shaping the product.

WO 03/097642 PCT/AU03/00569 - 14 By "cellular proliferative disorder" is meant that a cell or cells demonstrate abnormal growth, typically aberrant growth, leading to a neoplasm, tumour or a cancer. 5 Cellular proliferative disorders include, for example, cancers of the breast, lung, prostate, kidney, skin, neural, ovary, uterus, liver, pancreas, epithelial, gastric, intestinal, exocrine, endocrine, lymphatic, haematopoietic system or head and neck tissue. 10 Generally, neoplastic diseases are conditions in which abnormal proliferation of cells results in a mass of tissue called a neoplasm or tumour. Neoplasms have varying degrees of abnormalities in structure and behaviour. Some neoplasms are benign while others are 15 malignant or cancerous. An effective treatment of neoplastic disease would be considered a valuable contribution to the search for cancer preventive or curative procedures. The compounds of the invention are preferably used in the treatment of leukaemias, lymphomas, 20 multiple myeloma, sarcomas, and brain tumours, and for cancers of the lung, breast, ovary, testes, and colon. The term "subject" as used herein refers to any animal having a disease or condition which requires treatment with a pharmaceutically-active agent. The 25 subject may be a mammal, preferably a human, or may be a non-human primate or non-primates such as used in animal model testing. While it is particularly contemplated that the compounds of the invention are suitable for use in medical treatment of humans, it is also applicable to 30 veterinary treatment, including treatment of companion animals such as dogs and cats, and domestic animals such as horses, ponies, donkeys, mules, llama, alpaca, pigs, cattle and sheep, or zoo animals such as primates, felids, canids, bovids, and ungulates. 35 Suitable mammals include members of the Orders Primates, Rodentia, Lagomorpha, Cetacea, Carnivora, Perissodactyla and Artiodactyla. Members of the Orders WO 03/097642 PCT/AU03/00569 - 15 Perissodactyla and Artiodactyla are particularly preferred because of their similar biology and economic importance. For example, Artiodactyla comprises approximately 150 living species distributed through nine families: pigs 5 (Suidae), peccaries (Tayassuidae), hippopotamuses (Hippopotamidae), camels (Camelidae), chevrotains (Tragulidae), giraffes and okapi (Giraffidae), deer (Cervidae), pronghorn (Antilocapridae), and cattle, sheep, goats and antelope (Bovidae). Many of these animals are 10 used as feed animals in various countries. More importantly, many of the economically important animals such as goats, sheep, cattle and pigs have very similar biology and share high degrees of genomic homology. The Order Perissodactyla comprises horses and 15 donkeys, which are both economically important and closely related. Indeed, it is well known that horses and donkeys interbreed. As used herein, the term "therapeutically effective amount" is meant an amount of a compound of the 20 present invention effective to yield a desired therapeutic response, for example, to prevent or treat a cellular proliferative disorder. The specific "therapeutically effective amount" will, obviously, vary with such factors as the particular 25 condition being treated, the physical condition of the subject, the type of subject being treated, the duration of the treatment, the nature of concurrent therapy (if any), and the specific formulations employed and the structure of the compound or its derivatives. 30 The compounds of the present invention may additionally be combined with other medicaments to provide an operative combination. It is intended to include any chemically compatible combination of pharmaceutically active agents, as long as the combination does not 35 eliminate the activity of the compound of formula I, II or III. It will be appreciated that the compound of the WO 03/097642 PCT/AU03/00569 - 16 invention and the other medicament may be administered separately, sequentially or simultaneously. Other medicaments may include, for example, one or more other anti-neoplastic agents encompassing but not 5 limited to anti-mitotic agents such as taxol, anti metabolites such as 5-fluorouracil, hormonal regulators such as tamoxifen, DNA-reactive agents such as cisplatin, or biological agents such as interleukin-2 (IL-2) or antibodies. 10 A second DNA-binding anti-cancer therapeutic agent could be used in conjunction with administration of the compound of formula I, II or III in order to reduce toxic side effects or side affects to the recipient of either or both of the compound of formula I, II or III or 15 the other anti-cancer agent. The term "toxic side effects" or "side effects" means the deleterious, unwanted effects of chemotherapy on the subject's normal, non-diseased tissues and organs. For example, toxic side effects may include bone marrow 20 suppression (including neutropenia), cardiac toxicity, hair loss, gastrointestinal toxicity (including nausea and vomiting), neurotoxicity, lung toxicity and asthma. The aldehyde-releasing compound and/or chemotherapeutic agents may be administered orally, topically, or 25 parenterally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants, and vehicles. It is contemplated that compounds of the invention may also be administered in the form of tumour 30 activated prodrugs, in which the active agent is linked to a 'trigger' domain; such compounds may for example be designed to be activated by local hypoxia within a tumour mass. Suitable methods are known the in the art; see for example Denny, 1996; McFadyen et al, 1996. 35 The compound of the invention may also be used in combination with agents which relieve side effects caused by drug treatment such as granulocyte-macrophage- WO 03/097642 PCT/AU03/00569 - 17 colony stimulating factor (GM-CSF), or anti-emetics. As used herein, a "pharmaceutical carrier" is a pharmaceutically acceptable solvent, suspending agent or vehicle for delivering the compound of formula I, II or 5 III to the subject. The carrier may be liquid or solid and is selected with the planned manner of administration in mind. Each carrier must be pharmaceutically "acceptable" in the sense of being compatible with other ingredients of the composition and non injurious to the 10 subject. The compound of formula I, II or III may be administered orally, topically, or parenterally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants, and 15 vehicles. The term parenteral as used herein includes subcutaneous injections, aerosol for administration to lungs or nasal cavity, intravenous, intramuscular, intrathecal, intracranial, injection or infusion techniques. 20 The present invention also provides suitable topical, oral, and parenteral pharmaceutical formulations for use in the novel methods of treatment of the present invention. The compounds of the present invention may be administered orally as tablets, aqueous or oily 25 suspensions, lozenges, troches, powders, granules, emulsions, capsules, syrups or elixirs. The composition for oral use may contain one or more agents selected from the group of sweetening agents, flavouring agents, colouring agents and preserving agents in order to produce 30 pharmaceutically elegant and palatable preparations. Suitable sweeteners include sucrose, lactose, glucose, aspartame or saccharin. Suitable disintegrating agents include corn starch, methylcellulose, polyvinylpyrrolidone, xanthan gum, bentonite, alginic acid 35 or agar. Suitable flavouring agents include peppermint oil, oil of wintergreen, cherry, orange or raspberry flavouring. Suitable preservatives include sodium WO 03/097642 PCT/AU03/00569 - 18 benzoate, vitamin E, alphatocopherol, ascorbic acid, methyl paraben, propyl paraben or sodium bisulphite. Suitable lubricants include magnesium stearate, stearic acid, sodium oleate, sodium chloride or talc. Suitable 5 time delay agents include glyceryl monostearate or glyceryl distearate. The tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. 10 These excipients may be, for example, (1) inert diluents, such as calcium carbonate, lactose, calcium phosphate or sodium phosphate; (2) granulating and disintegrating agents, such as corn starch or alginic acid; (3) binding agents, such as starch, gelatin or 15 acacia; and (4) lubricating agents, such as magnesium stearate, stearic acid or talc. These tablets may be uncoated or coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer 20 period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. Coating may also be performed using techniques described in the U.S. Pat. Nos. 4,256,108; 4,160,452; and 4,265,874 to form osmotic therapeutic tablets for control 25 release. The compound of formula I, II or III as well as the pharmaceutically-active agent useful in the method of the invention can be administered, for in vivo application, parenterally by injection or by gradual 30 perfusion over time independently or together. Administration may be intravenously, intraarterial, intraperitoneally, intramuscularly, subcutaneously, intracavity, transdermally or infusion by, for example, osmotic pump. For in vitro studies the agents may be 35 added or dissolved in an appropriate biologically acceptable buffer and added to a cell or tissue.

WO 03/097642 PCT/AU03/00569 - 19 Preparations for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, 5 vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Parenteral vehicles include sodium chloride solution, 10 Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers (such as those based on Ringer's dextrose), and the like. Preservatives and other additives may also be present such as, for 15 example, anti-microbials, anti-oxidants, chelating agents, growth factors and inert gases and the like. Generally, the terms "treating", "treatment" and the like are used herein to mean affecting a subject, tissue or cell to obtain a desired pharmacological and/or 20 physiological effect. The effect may be prophylactic in terms of completely or partially preventing a disease or sign or symptom thereof, and/or may be therapeutic in terms of a partial or complete cure of a disease. "Treating" as used herein covers any treatment of, or 25 prevention of disease in a vertebrate, a mammal, particularly a human, and includes: (a) preventing the disease from occurring in a subject that may be predisposed to the disease, but has not yet been diagnosed as having it; (b) inhibiting the disease, i.e., arresting 30 its development; or (c) relieving or ameliorating the effects of the disease, i.e., cause regression of the effects of the disease. The invention includes various pharmaceutical compositions useful for ameliorating disease. The 35 pharmaceutical compositions according to one embodiment of the invention are prepared by bringing a compound of formula I, II or III, analogues, derivatives or salts WO 03/097642 PCT/AU03/00569 - 20 thereof, or combinations of compound of formula I, II or III and one or more pharmaceutically-active agents into a form suitable for administration to a subject using carriers, excipients and additives or auxiliaries. 5 Frequently used carriers or auxiliaries include magnesium carbonate, titanium dioxide, lactose, mannitol and other sugars, talc, milk protein, gelatin, starch, vitamins, cellulose and its derivatives, animal and vegetable oils, polyethylene glycols and solvents, such as sterile water, 10 alcohols, glycerol and polyhydric alcohols. Intravenous vehicles include fluid and nutrient replenishers. Preservatives include antimicrobial, anti-oxidants, chelating agents and inert gases. Other pharmaceutically acceptable carriers include aqueous solutions, non-toxic 15 excipients, including salts, preservatives, buffers and the like, as described, for instance, in Remington's Pharmaceutical Sciences, 20th ed., Williams and Wilkins, Pennsylvania, USA and The National Formulary XIV., 14th ed. Washington: American Pharmaceutical Association 20 (1975), the contents of which are hereby incorporated by reference. The pH and exact concentration of the various components of the pharmaceutical composition are adjusted according to routine skills in the art. See Goodman and Gilman's The Pharmacological Basis for Therapeutics (7th 25 ed., 1985). The pharmaceutical compositions are preferably prepared and administered in dose units. Solid dose units may be tablets, capsules and suppositories. For treatment of a subject, depending on activity of the compound, 30 manner of administration, nature and severity of the disorder, age and body weight of the subject, different daily doses can be used. Under certain circumstances, however, higher or lower daily doses may be appropriate. The administration of the daily dose can be carried out 35 both by single administration in the form of an individual dose unit or else several smaller dose units and also by WO 03/097642 PCT/AU03/00569 - 21 multiple administration of subdivided doses at specific intervals. The pharmaceutical compositions according to the invention may be administered locally or systemically in a 5 therapeutically effective dose. Amounts effective for this use will, of course, depend on the severity of the disease and the weight and general state of the subject. Typically, dosages used in vitro may provide useful guidance in the amounts useful for in situ administration 10 of the pharmaceutical composition, and animal models may be used to determine effective dosages for treatment of the cytotoxic side effects. Various considerations are described, e.g., in Langer, Science, 249: 1527, (1990). Formulations for oral use may be in the form of hard 15 gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin. They may also be in the form of soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, such as 20 peanut oil, liquid paraffin or olive oil. Aqueous suspensions normally contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspension. Such excipients may be (1) suspending agent such as sodium carboxymethyl 25 cellulose, methyl cellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; (2) dispersing or wetting agents which may be (a) naturally occurring phosphatide such as lecithin; (b) a condensation product of an alkylene oxide with a fatty 30 acid, for example, polyoxyethylene stearate; (c) a condensation product of ethylene oxide with a long chain aliphatic alcohol, for example, heptadecaethylenoxycetanol; (d) a condensation product of ethylene oxide with a partial ester derived from a fatty 35 acid and hexitol such as polyoxyethylene sorbitol monooleate, or (e) a condensation product of ethylene oxide with a partial ester derived from fatty acids and WO 03/097642 PCT/AU03/00569 - 22 hexitol anhydrides, for example polyoxyethylene sorbitan monooleate. The pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleagenous 5 suspension. This suspension may be formulated according to known methods using those suitable dispersing or wetting agents and suspending agents which have been mentioned above. The sterile injectable preparation may also a sterile injectable solution or suspension in a non 10 toxic parenterally-acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are 15 conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono-or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables. 20 Compounds of formula I, II or III may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as 25 cholesterol, stearylamine, or phosphatidylcholines. The compounds of formula I, II or III may also be presented for use in the form of veterinary compositions, which may be prepared, for example, by methods that are conventional in the art. Examples of 30 such veterinary compositions include those adapted for: (a) oral administration, external application, for example drenches (e.g. aqueous or non-aqueous solutions or suspensions); tablets or boluses; powders, granules or pellets for admixture with feed stuffs; pastes 35 for application to the tongue; (b) parenteral administration for example by subcutaneous, intramuscular or intravenous injection, e.g.

WO 03/097642 PCT/AU03/00569 - 23 as a sterile solution or suspension; or (when appropriate) by intramammary injection where a suspension or solution is introduced in the udder via the teat; (c) topical applications, e.g. as a cream, 5 ointment or spray applied to the skin; or (d) intravaginally, e.g. as a pessary, cream or foam. The term "therapeutically effective amount" means an amount of the compound of formula I, II or III of the 10 present invention effective to yield a desired therapeutic response. A "prophylactically effective amount" has a similar definition. Dosage levels of the compound of formula I, II or III of the present invention are of the order of about 15 0.5 mg to about 20 mg per kilogram body weight, with a preferred dosage range between about 0.5 mg to about 10 mg per kilogram body weight per day (from about 0.5 gms to about 3 gms per patient per day). The amount of active ingredient that may be combined with the carrier materials 20 to produce a single dosage will vary depending upon the host treated and the particular mode of administration. For example, a formulation intended for oral administration to humans may contain about 5 mg to lg of an active compound with an appropriate and convenient 25 amount of carrier material which may vary from about 5 to 95 percent of the total composition. Dosage unit forms will generally contain between from about 5 mg to 500 mg of active ingredient. Optionally the compounds of the invention are 30 administered in a divided dose schedule, such that there are at least two administrations in total in the schedule. Administrations are given preferably at least every two hours for up to four hours or longer; for example the compound may be administered every hour or every half 35 hour. In one preferred embodiment, the divided-dose regimen comprises a second administration of the compound of the invention after an interval from the first WO 03/097642 PCT/AU03/00569 - 24 administration sufficiently long that the level of active compound in the blood has decreased to approximately from 5-30% of the maximum plasma level reached after the first administration, so as to maintain an effective content of 5 active agent in the blood. Optionally one or more subsequent administrations may be given at a corresponding interval from each preceding administration, preferably when the plasma level has decreased to approximately from 10-50% of the immediately-preceding maximum. 10 It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of 15 administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 shows the structures of prior art 20 compounds 1 to 15 referred to herein. Figure 2 shows reaction schemes for the preparation of the compounds of Table 1, namely, Scheme 2a - Route to 4H-pyrano[4,3-b]quinoline-l,3-diones; Scheme 2b - Reaction with amines; Scheme 2c - Reaction with 3,4 25 dimethoxyaniline; Scheme 2d - Preparation of carboxamides; and Schemes 2e and 2f - Preparation of intermediate bis amides and their hydrolysis to mono amides. Figure 3 is a graph showing a comparison between the growth of transplanted tumour in the colon 38 in 30 control mice (0) and those receiving a single dose of 20d (8.9 mg/kg; O) (3.9 mg/kg; A). Figure 4 is a graph showing a comparison between the growth of NZM4 melanoma cell line implanted subcutaneously in control mice (0) and those receiving 20d 35 in two doses of 5.9 mg/kg, administered 7 days apart (A).

WO 03/097642 PCT/AU03/00569 - 25 EXAMPLES The invention will now be described with reference to the following examples. These examples are not to be construed as limiting the invention in any way. 5 The structure of representative compounds of the invention is summarised in Table 1. It will be evident that forms A and B represent compounds of general formula I, and II, respectively. 10 Table 1 0 9 10 8 *- \

N

2 z 2 7 'N^ - 3 44 15 6 5 CONHR A 20 9 1 0 01 10 109 8 \ \ N- Y- N / / 8 7 /3 3 N 7 6 54 4Y 5 6 25 CONHR RHNOC

B

WO 03/097642 PCT/AU03/00569 - 26 Representative Compounds of the Invention a Cpd Fm Y Z 20b A Me H 20c A H 6-Me 20d A Me 6-Me 20e A Et 6-Me 20f A Bu 6-Me 20g A CH 2

CF

3 6-Me 20h A (CH 2

)

2 0H 6-Me 20i A CH 2

CO

2 Et 6-Me 20j A (CH 2

)

3

CO

2 Et 6-Me 20k A (CH 2

)

2 NMe 2 6-Me 201 A (CH 2 ) 2 NMe 2 H 20m A CH(Me)Ph-(s) 6-Me 20n A Ph 6-Me 20o A C 6

H

4 F-4 6-Me 20p A C 6

H

4 B (OH) 2 -4 6-Me 20q A C 6

H

3 [3,4-(MeO) 2 ] 6-Me 20r A CH 2

C

6

H

3 [3,4- (MeO) 2] 6-Me 20s A (CH 2 ) 2

C

6

H

3 [3,4- (MeO) 2] 6-Me 20t A CH 2 (2-pyridinyl) 6-Me 20u A (CH 2 ) 3 (N-(2-oxopyrrolidinyl)) 6-Me 20v A (CH 2 ) 2 (3-indolyl) 6-Me 20w A Me 7-MeO 20x A Me 6-C1-7-MeO 20y A Me 6-aza 20z A Me 10-aza 21a A (CH 2 )20CONH(CH 2

)

2 NMe 2 6-Me H N O --C' NMe 21b A N 2 6-Me (CH ) 20aa B (CH 2 ) 2 NMe (CH 2 ) 3 NMe (CH 2 ) 2 6-Me 20bb A Me 6-Me 20cc A Me 6-Me WO 03/097642 PCT/AU03/00569 - 27 Footnotes a R = (CH 2 )2NMe 2 except 20bb [(S)-CH(Me)CONMe 2 ], 20cc [(S) 5 CH(Me)CH 2 NMe 2 ] Chemistry The homophthalic acid analogues 16a-d were prepared by adapting a method for the pyridine example 10 (Ames and Dodds, 1972). Analogue 16e was prepared by another literature procedure (Kubo et al, 1986). Reaction of 16 with Vilsmeier reagent gave compounds 17 (Scheme 2a) (Deady and Rodemann, 2001). Compound 18d was formed previously by refluxing 17b with phosphoryl chloride for 15 48 h (Deady and Rodemann, 2001). However, reaction with methylamine in tetrahydrofuran/dimethylformamide at room temperature brought about the same conversion in an experimentally preferable procedure; the other 17 reacted similarly. The related reaction of isocoumarins with 20 ammonia to give isoquinolones [the Gabriel reaction (Gabriel, 1885)] is known and has been extended in related systems to reactions with alkyl (Jhalani et al, 1989) and aryl (Modi and Usgaonkar, 1979) amines. Compounds 17 reacted with a wide selection of amines under very mild 25 conditions, to give 18 (Scheme 2b) in generally good yields, while reaction of 17b with N,N'-bis-(2 aminoethyl)-N,N'-dimethylpropane-l,3-diamine gave an example of a bis compound 18aa. A slight anomaly occurred when ammonia was used as 30 the alkyl amine, as the product which separated from the reaction mixture was the ammonium salt of the acid; liberation of 18c required treatment with acid. Reaction of 17 with arylamines failed in the absence of triethylamine, and it seems that moderate base 35 catalysis of this reaction is required. Alkyl amines behave as both nucleophile and base, but arylamines required the presence of the more basic, non-nucleophilic WO 03/097642 PCT/AU03/00569 - 28 triethylamine. Even so, a complication arose because the dimethylamine liberated during the reaction competed with the arylamine and led to formation of a byproduct. This was minimised by using a large excess of the arylamine. In 5 the case of 3,4-dimethoxyaniline, this complication did not arise because the initial product, 19, was very insoluble. To get the completed reaction, reflux in pyridine for 8 h of a mixture of 19, more 3,4 dimethoxyaniline and triethylamine was required, and gave 10 18q in 78% yield (Scheme 2c). The carboxamides of Table 1 were prepared by reaction of the intermediate acid chlorides (from thionyl chloride reaction with 18, and not isolated) or imidazolides (from l,l'-carbonyldiimidazole reaction with 18, and not 15 isolated) with the appropriate amine. Experimental NMR spectra were recorded on a Bruker AM-300 spectrometer operating at 300.13 MHz ('H) and 75.47 MHz 20 (1 3 C) and a Bruker DRX-400 spectrometer operating at 400.13 MHz ('H) and 100.62 MHz (13C) and chemical shifts are reported as 6 values (ppm) relative to Me 4 Si. COSY spectra were recorded on the AM-300 spectrometer using the pulse program COSY.AUR from the Bruker library. HMQC and HMBC 25 spectra were recorded on the DRX-400 spectrometer using the pulse programs INV4GSTP and INV4GSLPLRND, respectively. NOESY spectra were recorded on the Bruker DRX-400 spectrometer using the pulse program NOESYTP from the Bruker library. Various standard techniques were used 30 to identify proton-bound carbons in 13 C NMR spectra. Electrospray mass spectra (ESMS) were recorded on a VG Bio-Q triple quadrupole Mass Spectrometer using methanol or acetonitrile with formic acid (1%) as mobile phase. El and LSI (3-nitrobenzyl alcohol as liquid matrix) mode 35 high-resolution mass spectra were obtained by Dr N. Davies, University of Tasmania, Australia. Microanalyses WO 03/097642 PCT/AU03/00569 - 29 were performed at the Campbell Microanalytical Laboratory, University of Otago, New Zealand. N,N'-Bis-(2-aminoethyl)-N,N'-dimethylpropane 1,3-diamine was available (Deady et al, 2000) and the 5 other amines were commercial samples. Precursor compounds 16a-d were prepared by adapting a method for the pyridine analogue (Ames and Dodds, 1972): Ethyl (3-Carboxyquinolin-2-yl)acetate (16a), 10 obtained as a beige solid, mp 170-171 OC (from acetonitrile). IH NMR (CDC1 3 ): 6 1.24 (t, 3H, J=7.1 Hz), 4.20 (q, 2H, J=7.1 Hz), 4.57 (s, 2H), 7.58 (t, 1H, J=7.6 Hz), 7.82 (t, 1H, J=7.5 Hz), 7.91 (d, 1H, J=8.0 Hz), 8.19 (d, 1H, J=8.5 Hz), 9.00 (s, 1H), 12.28 (br s, 1H). 15 Ethyl (3-Carboxy-8-methylquinolin-2-yl)acetate (16b), as reported (Deady and Rodemann, 2001) Ethyl (3-Carboxy-7-methoxyuinolin-2-yl)acetate (16c), obtained as an orange solid, mp 193-195 oC (dec) (from ethanol). Occasionally, the compound was obtained as 20 a red oil, which solidified on trituration with cold acetonitrile. Recrystallization was not necessary prior to use in the next step. 'H NMR (CDC1 3 ): 6.1.22 (t, J=7.1 Hz, 3H, C0O 2

CH

2

CH

3 ), 4.02 (s, 3H, ArOCH 3 ), 4.16 (q, J=7.1 Hz, 2H,

CO

2

CH

2

CH

3 ), 4.65 (s, 2H, CH 2

CO

2 Et), 7.33 (dd, J=9.1, 1.8 Hz, 25 1H), 7.88 (m, 2H), 9.05 (s, 1H). Ethyl (3-Carboxyquinoxalin-2-yl)acetate (16d), obtained as a brown semi-solid, which was used in this state in the next step. Ethyl (3-Ethoxycarbonyl[1,8]naphthyridin-2 30 yl)acetate (16e) was prepared from 2-aminonicotinaldehyde and diethyl 1,3-acetonedicarboxylate by the method reported for the dimethyl analogue (Kubo et al, 1986), as an orange solid, mp 79-80 oC (from ethyl acetate). H NMR (CDC1 3 ): 6 1.20 (t, J=7.1 Hz, 3H, 2-CO 2

CH

2

CH

3 ), 1.39 (t, 35 J=7.1 Hz, 3H, 3-CO 2

CH

2

CH

3 ), 4.13 (q, J=7.1 Hz, 2H, 2

CO

2

CH

2

CH

3 ), 4.38 (q, J=7.1 Hz, 2H, 3-CO 2

CH

2

CH

3 ), 4.49 (s, 2H, CH 2

CO

2 Et), 7.52 (dd, J=8.0, 4.2 Hz, IH, H-6), 8.27 (d, WO 03/097642 PCT/AU03/00569 - 30 J=7.9 Hz, 1H, H-5), 8.84 (s, 1H, H-4), 9.15 (d, J=2.4 Hz, 1H, H-7). Example 1: Preparation of 4-Dimethylaminomethylene-6 5 methyl-4H-pyrano[4,3-b]quinoline-1,3-dione (17b). This is an example of the general preparation of the diones of Formula V. Phosphorus oxychloride (13 mL) was added, with 10 stirring at 0 oC, to dimethylformamide (40 mL). After a further 20 min., a solution of ethyl (3-carboxy-8 methylquinolin-2-yl)acetate 16b (10.0 g) in dimethylformamide (10 mL) was added in a single portion and the mixture was stirred at room temperature for a 15 further 2 h. The precipitate was collected by filtration and washed with cold acetone to give the product as an orange solid (10.0 g, 97%), mp >295 OC (decomposed-formed needles above 280 OC). 1 H NMR (d 6 -DMSO): 6 2.69 (s, 3H,

CH

3 ), 3.30 (s, 3H, N-CH 3 ), 3.59 (s, 3H, N-CH 3 ), 7.36 (t, 20 1H, J=7.6 Hz, H-3), 7.68 (d, IH, J=7.0 Hz), 7.89 (d, IH, J=8.1 Hz), 8.81 (s, IH), 8.93 (s, 1H). The following compounds were prepared using a similar procedure: 4-Dimethylaminomethylene-4H-pyrano[4,3 25 b]quinoline-1,3-dione (17a) From 16a, and obtained as an orange solid (87%), mp >300 oC. IH NMR (d 6 -DMSO): 8 3.33 (s, 3-H, N-CH 3 ), 3.64 (s, 3-H, N-CH 3 ), 7.55 (t, 1-H, J=7.4 Hz), 7.91 (t, 1-H, J=7.3 Hz), 8.08 (d, 1-H, J=7.9 Hz), 8.15 (d, 1-H, J=7.8 30 Hz), 8.94 (s, 1-H), 9.13 (s, 1-H). 4-Dimethylaminomethylene-7-methoxy-4H-pyrano[4,3 b]quinoline-1,3-dione (17c) From 16c, and obtained as a bright yellow solid (95%), mp 273-277 0C (after forming needles >230 oC). 1 H NMR 35 (d 6 -DMSO): 8 3.33 (s, 3H, NCH 3 ), 3.62 (s, 3H, NCH 3 ), 3.94 (s, 3H, ArOCH 3 ), 7.18 (dd, J=8.9, 1.7 Hz, 1H), 7.57 (s, 1H), 8.03 (d, J=9.0 Hz, 1H), 8.97 (s, 2H).

WO 03/097642 PCT/AU03/00569 - 31 4-Dimethylaminomethylene-4H-pyrano[3,4-b]quinoxaline 1,3-dione (17d) From crude 16d, with a changed ratio of 1 g 16d: 0.8 mL POC1 3 : 1.6 mL DMF. The reaction mixture was heated, 5 with moisture exclusion, at 75 OC for 16 h, then cooled, diluted with cold dichloromethane, and kept at -18 OC for 1 h. The brick red solid was filtered and washed exhaustively, with thorough stirring, with cold dichloromethane to give the dione (22% from 3 10 chloroquinoxaline-2-carboxylic acid), mp 133-139 OC. 1H NMR (d 6 -DMSO): 8 3.27 (s, 3H, NCH 3 ) , 3.57 (s, 3H, NCH 3 ), 7.66 (ddd, J=8.3, 6.6, 1.6 Hz, 1H), 7.81-7.92 (m, 2-H), 8.04 (d, J=7.8 Hz, 1H), 8.66 (s, 1H, =HCNMe 2 ). 13 C NMR (d 6 -DMSO): 6 45.0 (CH 3 ), 48.3 (CH 3 ), 87.0 (C), 127.2 (CH), 128.3 (CH), 15 130.3 (CH), 133.3 (C), 133.7 (CH), 139.4 (C), 143.2 (C), 150.3 (C), 157.6 (C), 159.7 (C), 161.1 (CH). 9-Dimethylaminomethylene-9H-pyrano[4,3 b][1,8]naphthyridine-6,8-dione (17e) From diester 16e, with a changed ratio of 1 g 20 16e: 0.8 mL POC1 3 : 1.6 mL DMF. The reaction mixture was heated, with moisture exclusion, at 75 OC for 16 h, then cooled, diluted with cold dichloromethane, and kept on ice for 1 h. The orange solid was filtered and washed exhaustively, with thorough stirring, with cold 25 dichloromethane. The mass of orange dione obtained represented >100% yield. It was used in further reaction in this state within 24 h, since it decomposed on long standing. H NMR (d 6 -DMSO): 6 3.33 (s, 3H, NCH 3 ), 3.62 (s, 3H, NCH 3 ), 7.67 (dd, J=7.8, 4.9 Hz, 1H), 8.77 (s, 1H), 8.82 30 (d, J=8.1 Hz, 1H), 9.07 (d, J=4.9 Hz, 1H), 9.15 (s, 1H).

WO 03/097642 PCT/AU03/00569 - 32 Example 2: Preparation of 2,6-Dimethyl-1-oxo-l,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18d). This is an example of the general preparation of 5 the carboxylic acids of Formula IV from precursor compounds of Formula V by reaction with an excess of an amine according to Scheme 2b. A solution of methylamine in tetrahydrofuran (7.0 ml, 2 M) was added to a suspension of 17b (0.8 g) in 10 dimethylformamide (20 mL) and the whole was stirred for 16 h at room temperature. The solid was collected by filtration and washed with cold acetone to give the product as a bright yellow solid (0.60 g, 79%), mp >300 oC (formed cubic crystals above 290 oC). 'H NMR (d 6 -DMSO) : 8 15 2.75 (s, 3H, CH 3 ), 3.67 (s, 3H, N-CH 3 ), 7.67 (t, 1H, J=7.7 Hz, H-8), 7.95 (d, 1H, J=6.6 Hz), 8.25 (d, 1H, J=8.1 Hz), 8.83 (s, 1-H), 9.52 (s, 1-H), 16.03 (s, 1H, COOH). Anal. Calc. for C1 5

HI

2

N

2 0 3 -0.2H 2 0: C, 66.3; H, 4.6; N, 10.3. Found: C, 66.6; H, 4.4; N, 10.4. 20 The following compounds were made using a similar procedure. 2-Methyl-1-oxo-l,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18b). From 17a and methylamine, as for 18d, and 25 obtained as a yellow solid (69%), mp >305 oC (formed needles >230 OC. IH NMR (d 6 -DMSO) : 8 3.61 (s, 3H, N-CH 3 ), 7.71 (t, IH, J=7.7 Hz), 8.00 (t, 1H, J=8.2 Hz), 8.16 (d, 1H, J=8.6 Hz), 8.33 (d, 1H, J=8.1 Hz), 8.66 (s, 1-H), 9.46 (s, 1-H), 16.00 (s, 1H, COOH). 30 2-Butyl-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18f). From 17b and butylamine, as for 18d, and obtained as a bright yellow solid (82%), mp 259 OC. 'H NMR (CDC1 3 ): 5 0.98 (t, 3H, J=7.2 Hz, (Bu)CH 3 ), 1.42 (sextet, 35 2H, J=7.6 Hz, (Bu)CH 2 ), 1.81 (quintet, 2H, J=7.7 Hz, (Bu)CH 2 ), 2.83 (s, 3H, C6-CH 3 ), 4.10 (t, 2H, J=7.4 Hz, N

CH

2 ), 7.57 (t, 1H, J= 7.7 Hz, H-8), 7.81 (d, 1H, J=7.0 Hz, WO 03/097642 PCT/AU03/00569 - 33 H-7), 7.93 (d, 1H, J=8.3 Hz, H-9), 8.59 (s, 1H, H-3), 9.35 (s, 1H, H-10), 16.09 (br s, IH, COOH). C NMR (CDC1 3 ): 6 13.6 (y-CH 3 ), 18.2 (C6-CH 3 ), 19.9 (-CH 2 ), 31.3 (t-CH 2 ), 49.8 (N-CH 2 ), 105.2 (C-4), 119.1 (C-10a), 126.4 (C-9a), 5 127.4 (C-8), 127.6 (C-9), 134.2 (C-7), 134.9 (C-6), 141.3 (C-10), 145.2 (C-3), 146.7 (C-5a), 148.7 (C-4a), 161.8 (C 1), 166.3 (COOH). 2-[(2-Dimethylamino)ethyl] -6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18k). 10 From 17b and N,N-dimethylethylenediamine, as for 18d, and obtained as a yellow solid (78%), mp 254-255 OC. 1H NMR (d 6 -DMSO): 6 2.21 (s, 6H, N(CH 3

)

2 ), 2.60 (t, 2H, J=6.0 Hz), 2.73 (s, 3H, CH 3 ), 4.23 (t, 2H, J=6.0 Hz), 7.65 (t, 1H, J=7.6 Hz, H-8), 7.93 (d, 1H, J=6.8 Hz), 8.21 (d, 15 1H, J=8.3 Hz), 8.74 (s, 1H, H-3), 9.48 (s, 1H, H-10). 2-[(2-Dimethylamino)ethyl] -1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (181). From 17a and N,N-dimethylethylenediamine, as for 18d, and obtained as a yellow solid (52%), mp 232-233 OC. 20 'H NMR (d 6 -DMSO): 6 2.20 (s, 6H, N(CH3)2), 2.58 (t, 2H, J=5.9 Hz), 4.22 (t, 2H, J=6.0 Hz), 7.77 (t, 1H, J=7.4 Hz), 8.06 (t, IH, J=7.6 Hz), 8.23 (d, 1H, J=8.6 Hz), 8.39 (d, 1H, J=8.3 Hz), 8.74 (s, 1H, H-3), 9.54 (s, 1H, H-10). (S)-6-Methyl-1-oxo-2- (1-phenylethyl) -1,2 25 dihydrobenzo[b] [l,6]naphthyridine-4-carboxylic acid (18m). From 17b and(S)-(-)-a-methylbenzylamine, as for 18d except that the product remained in the dimethylformamide solution. Water was added to the reaction mixture and the resulting precipitate was 30 collected by filtration and washed with water to give the product as a yellow solid (79%), mp 234-235 0C. :H NMR (d 6 -DMSO): 6 1.84 (d, 3H, J=7.1 Hz, CH-CH3), 2.73 (s, 3H, C6-CH3), 6.29 (q, 1H, J=7.1 Hz, N-CH), 7.3-7.5 (m, 5-H), 7.66 (t, 1H, J=7.7 Hz, H-8), 7.94 (d, 1H, J=6.8 Hz), 8.23 35 (d, 1H, J=8.2 Hz), 8.43 (s, 1-H), 9.53 (s, 1H, H-10), 16.00 (br s, 1H, COOH).

WO 03/097642 PCT/AU03/00569 - 34 7-Methoxy-2-methyl-1-oxo-1,2-dihydro benzo[b][1,6]naphthyridine-4-carboxylic acid (18w) From 17c and methylamine, as for 18d, and obtained as a yellow solid (86%), mp >300 'C (from dimethyl 5 sulfoxide/l,4-dioxane). 'H NMR (d 6 -DMSO): 8 3.61 (s, 3H,

NCH

3 ), 3.99 (s, 3H, ArOCH 3 ), 7.34 (d, J=8.0 Hz, 1H, H-8), 7.54 (s, 1H, H-6), 8.21 (d, J=9.1 Hz, 1H, H-9), 8.73 (s, 1H, H-3), 9.31 (s, 1H, H-10), 15.82 (s, 1H, COOH). 7-Methyl-6-oxo-6,7-dihydropyrido[2,3 10 b][1,6]naphthyridine-9-carboxylic acid (18y) From 17e and methylamine, as for 18d except that, after reaction, the volatiles were removed at reduced pressure and water was added. The resultant suspension was stirred and basified with 10% sodium hydroxide, then 15 acidified with concentrated hydrochloric acid (dropwise) and the brown solid was filtered to give 18y, mp >300 oC (from dimethyl sulfoxide/1,4-dioxane). 1H NMR (d 6 -DMSO): 8 3.64 (s, 3H, NCH 3 ), 7.76 (dd, J=8.2, 4.2 Hz, 1H, H-3), 8.81 (d, J=8.2 Hz, 1H, H-4), 8.85 (s, 1H, H-8), 9.28 (dd, 20 J=3.9, 1.7 Hz, 1H, H-2), 9.58 (s, 1H, H-5), 15.68 (s, 1-H, COOH). 2-Methyl-1-oxo-1,2-dihydropyrido[3,4 b]quinoxaline-4-carboxylic acid (18z) From 17d and methylamine, as for 18d except that, 25 after reaction, the volatiles were removed at reduced pressure to leave the methylamine salt of 18z as a yellow brown solid. This was suspended in a small amount of water and 10% sodium hydroxide was added dropwise, with stirring, until a solution was obtained. This was 30 acidified with a minimum amount of concentrated hydrochloric acid and the solid which separated was filtered and washed with water to give the free acid as a yellow solid (52%), mp >300 OC (from dimethyl sulfoxide/l,4-dioxane). 'H NMR (d 6 -DMSO): 8 3.66 (s, 3H, 35 NCH 3 ), 7.97 (t, J=7.5 Hz, 1H), 8.08 (t, J=7.6 Hz, 1H), 8.25-8.32 (m, 2H), 8.79 (s, 1H), 14.03 (br s, 1H, COOH).

WO 03/097642 PCT/AU03/00569 - 35 6-Methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18c). A mixture of 17b (1.0 g) in dimethylformamide (25 mL) was stirred under a stream of ammonia for 4 h at 5 room temperature. The precipitate, which formed after dissolution of the starting material, was collected by filtration and washed thoroughly with acetone to give the ammonium salt of the product as a yellow solid (0.70 g, 78 %), mp >300 oC (after forming needles >255 oC). 'H NMR 10 (d 6 -DMSO/CDC1 3 ): 8 2.75 (s, 3H, CH 3 ), 7.16 (t , 4H, J=51.1 Hz, NH 4 +), 7.58 (t, 1H, J=7.6 Hz, H-8), 7.84 (d, 1H, J=6.9 Hz), 8.07 (d, 1H, J=8.3 Hz), 8.37 (d, 1H, J=6.5 Hz, H-3), 9.37 (s, 1H, H-10), 12.20 (br s, 1H, NH), 16.00 (br s, 1H, COOH). 15 * Triplet of equal height The ammonium salt (0.15 g) in 5% hydrochloric acid (20 mL) was heated under reflux until a clear solution was obtained (10 min). After being cooled, the solution was adjusted to pH 4 with 10% sodium hydroxide. The 20 precipitate was collected by filtration to give the acid as a yellow solid (0.13 g, 93%), mp >300 OC (after forming needles >255 oC). IH NMR (d 6 -DMSO): 6 2.67 (s, 3H, CH 3 ), 7.58 (br s, 1H, H-8), 7.89 (d, 1H, J=4.9 Hz), 8.14 (d, lH, J=7.0 Hz), 8.31 (s, 1H, H-3), 9.35 (s, 1H, H-10), 12.19 25 (br s, 1H, NH), 15.90 (br s, 1H, COOH). Example 3: Preparation of 6-Methyl-1-oxo-2-(2,2,2 trifluoroethyl)-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic 30 acid (18g) This is an example of the general preparation of the carboxylic acids of Formula IV from precursor compounds of Formula V by reaction with an amine in the presence of an excess of triethylamine according to Scheme 35 2b. To a stirring suspension of dione 17b (1.00 g, 3.54 mmol) in N,N-dimethylformamide (15 mL) was added WO 03/097642 PCT/AU03/00569 - 36 triethylamine (2.5 mL) with constant stirring. 2,2,2 Trifluoroethylamine (0.40 g, 4.04 mmol) was added and the whole was stirred at room temperature for 16 h. The solid was filtered and washed with water to give the yellow acid 5 (64%), mp 297-300 oC (formed needles at 234-235 oC). :H NMR (d 6 -DMSO): 6 2.67 (s, 3H, ArCH 3 ), 5.12 (q, J=9.0 Hz,

CH

2

CF

3 ), 7.63 (t, J=7.6 Hz, 1H, H-8), 7.90 (d, J=6.9 Hz, 1H, H-7), 8.17 (d, J=8.2 Hz, 1H, H-9), 8.81 (s, 1H, H-3), 9.46 (s, 1H, H-10), 15.91 (br s, 1H, COOH). 10 The following compounds were made using a similar procedure. 2-Ethyl-6-methyl-1-oxo-1,2-dihydro benzo[b] [1,6]naphthyridine-4-carboxylic acid (18e). From 17b and ethylamine, as for 18d except that 15 the initial addition of ethylamine was carried out at 0 OC and a positive pressure of nitrogen was maintained throughout, and obtained as a yellow solid (72%), mp 251 253 OC. 1 H NMR (d 6 -DMSO): 8.1.30 (t, J=7.1 Hz, 3H, CH 2

CH

3 ), 2.65 (s, 3H, ArCH 3 ), 4.12 (q, J=7.1 Hz, 2H, CH 2

CH

3 ), 7.59 20 (t, J=7.6 Hz, 1H, H-8), 7.86 (d, J=6.8 Hz, 1H, H-7), 8.12 (d, J= 8.3 Hz, IH, H-9), 8.74 (s, 1H, H-3), 9.36 (s, IH, H-10), 15.89 (s, H-1, COOH). 2-(2-Hydroxyethyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18h). 25 From 17b and ethanolamine, as for 18g, and obtained as a yellow solid (64%), mp 258-261 OC. 'H NMR (d 6 -DMSO): 8 2.64 (s, 3H, ArCH 3 ), 3.70 (t, J=6.2 Hz, 2H,

CH

2

CH

2 OH), 4.15 (t, J=5.0 Hz, 2H, CH 2

CH

2 OH), 7.57 (t, J=7.6 Hz, IH, H-8), 7.85 (d, J=6.8 Hz, 1H, H-7), 8.11 (d, J=8.3 30 Hz, 1H, H-9), 8.62 (s, 1H, H-3), 9.35 (s, IH, H-10), 15.85 (s, 1H, COOH). 2-(Ethoxycarbonylmethyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18i). From 17b, triethylamine and glycine ethyl ester 35 hydrochloride, as for 18g except that water was added to the reaction mixture before the acid was filtered. The product was obtained as a yellow solid (65%), mp 277-280 WO 03/097642 PCT/AU03/00569 - 37 0 C. 'H NMR (d 6 -DMSO): 1.20 (t, J=7.1 Hz, 3H, CO 2

CH

2

CH

3 ), 2.69 (s, 3H, ArCH 3 ), 4.17 (q, J=7.1 Hz, 2H, CO 2

CH

2

CH

3 ), 4.97 (s, 2H, CH 2

CO

2 Et), 7.67 (t, J=7.7 Hz, 1H, H-8), 7.91 (d, J=6.9 Hz, 1H, H-7), 8.18 (d, J=8.2 Hz, 1H, H-9), 8.85 (s, 5 1H, H-3), 9.44 (s, 1H, H-10), 15.89 (br s, 1H, COOH). 2-(3-Ethoxycarbonylpropyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18j). From 17b and ethyl 4-aminobutyrate hydrochloride, as for 18g except that 1,4-dioxane was used 10 as solvent. After 16 h, water was added to the reaction mixture and the yellow solid was filtered and washed with a little 3% hydrochloric acid, then with water to give the acid 18j (59%), mp 186-188 'C. This was used in the next step without further purification but can be 15 recrystallized from toluene as a yellow solid. H NMR (CDC13): 8 1.23 (t, J=7.1 Hz, 3H, CO 2

CH

2

CH

3 ), 2.15 (quintet, J=7.1 Hz, 2H, CH 2

CH

2

CH

2 ), 2.41 (t, J=7.1 Hz, 2H,

NCH

2

CH

2

CH

2 ), 2.76 (s, 3H, ArCH 3 ), 4.07-4.18 (m, 4H, CO 2

CH

2

CH

3 and NCH 2

CH

2

CH

2 ), 7.54 (t, J=7.6 Hz, 1H, H-8), 7.77 (d, 20 J=6.9 Hz, 1H, H-7), 7.87 (d, J=8.3 Hz, 1H, H-9), 8.57 (s, 1H, H-3), 9.25 (s, 1H, H-10), 15.93 (s, 1H, COOH). 13C NMR (CDC1 3 ): 6 14.1 (CO 2

CH

2

CH

3 ), 18.1 (ArCH 3 ), 24.3 (CH 2

CH

2

CH

2 ), 31.1 (NCH 2

CH

2

CH

2 ), 49.3 (NCH 2

CH

2

CH

2 ), 60.7 (CO 2

CH

2

CH

3 ), 105.3 (C-4), 118.9 (C-10a), 126.4 (C-9a), 127.4 (CH, C-8), 127.6 25 (CH, C-9), 134.3 (CH, C-7), 134.8 (C-6), 141.3 (CH, C-10), 145.2 (CH, C-3), 146.5 (C-5a), 148.5 (C-4a), 161.7 (C-l), 166.0 (COOH) , 172.1 (CO 2 Et). 2-(3,4-Dimethoxybenzyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18r). 30 From 17b and veratrylamine, as for 18g, and obtained as a yellow solid (77%), mp 256-257 oC. H NMR (d 6 DMSO): 6 2.70 (s, 3H, ArCH 3 ), 3.69 (s, 3H, OCH 3 ), 3.70 (s, 3H, OCH 3 ), 5.25 (s, 2H, CH 2 Ph), 6.88-6.97 (m, 2H, H-5',H 6'), 7.07 (s, 1H, H-2'), 7.63 (t, J=7.6 Hz, 1H, H-8), 7.90 35 (d, J=7.0 Hz, 1H, H-7), 8.19 (d, J=8.2 Hz, 1H, H-9), 8.81 (s, 1H, H-3), 9.49 (s, 1H, H-10), 15.97 (br s, 1H, COOH).

WO 03/097642 PCT/AU03/00569 - 38 2-[2-(3,4-Dimethoxyphenyl)ethyl]-6-methyl-1-oxo 1,2-dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18s). From 17b and 3,4-dimethoxyphenethylamine, as for 5 18g, and obtained as a yellow solid (79%), mp 280-282 oC. 1 H NMR (d 6 -DMSO): 6 2.70 (s, 3H, ArCH 3 ), 2.94 (t, J=7.3 Hz, 2H, CH 2

CH

2 Ph), 3.66 (s, 3H, OCH 3 ), 3.67 (s, 3H, OCH 3 ), 4.31 (t, J=7.2 Hz, 2H, CH 2

CH

2 Ph), 6.71 (d, J=8.1 Hz, 1H, H-5'), 6.80 (d, J=8.1 Hz, 1H, H-6'), 6.91 (s, IH, H-2'), 7.64 (t, 10 J-=7.8 Hz, IH, H-8), 7.92 (d, J=6.8 Hz, 1H, H-7), 8.21 (d, J=8.4 Hz, 1H, H-9), 8.67 (s, 1H, H-3), 9.48 (s, 1H, H-10), 15.89 (br s, IH, COOH). 6-Methyl-1-oxo-2-(pyridin-2-yl)methyl-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18t). 15 From 17b and 2-aminomethylpyridine, as for 18g, and obtained as a yellow solid (57%), mp >300 oC. :H NMR (d 6 -DMSO): 6 2.72 (s, 3H, ArCH 3 ), 5.47 (s, 2H, CH 2 Pyr), 7.27 (dd, J=7.0, 5.2 Hz, 1H), 7.44 (d, J=7.8 Hz, 1H), 7.63 (t, J=7.6 Hz, 1H), 7.78 (t, J=7.7 Hz, 1H), 7.92 (d, J=6.7 Hz, 20 1H), 8.18 (d, J=8.3 Hz, 1H), 8.44 (d, J=4.6 Hz, 1H), 8.90 (s, 1H, H-3), 9.43 (s, 1H, H-10), 15.98 (br s, 1H, COOH). 6-Methyl-1-oxo-2- [3-(2-oxopyrrolidin-1 yl)propyl] -1,2-dihydrobenzo [b] [1,6] naphthyridine-4 carboxylic acid (18u). 25 From 17b and N-(3-aminopropyl)-2-pyrrolidinone, as for 18g, and obtained as a yellow solid (0.43 g, 64%), mp 226-228 oC. 1 H NMR (d 6 -DMSO): 6 1.88-1.89 (m, 4H), 2.20 (t, J=8.0 Hz, 2H), 2.56 (s, 3H, ArCH 3 ), 3.22-3.38 (m, 4H), 4.04 (t, J=7.3 Hz, 2H), 7.54 (t, J=7.7 Hz, 1H, H-8), 7.81 (d, 30 J=7.0 Hz, 1H, H-7), 8.04 (d, J=8.2 Hz, 1H, H-9), 8.74 (s, 1H, H-3), 9.29 (s, 1H, H-10), 15.76 (br s, 1H, COOH). 2-[2-(1H-Indol-3-yl)ethyl]-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18v). From 17b and tryptamine, as for 18g, and 35 obtained as a yellow solid (91%), mp >300 C. 'H NMR (d 6 DMSO): 6 2.69 (s, 3H, ArCH 3 ), 3.14 (t, J=7.2 Hz, 2H,

CH

2

CH

2 Indole), 4.36 (t, J=7.1 Hz, 2H, CH 2

CH

2 Indole), 6.94 WO 03/097642 PCT/AU03/00569 - 39 (t, J=7.4 Hz, 1H), 7.02 (t, J=7.5 Hz, 1H), 7.14 (s, 1H, H 2'), 7.30 (d, J=8.0 Hz, 1H), 7.58-7.65 (m, 2H), 7.90 (d, J=6.8 Hz, 1H), 8.19 (d, J=7.9 Hz, 1H), 8.54 (s, 1H, H-3), 9.49 (s, 1H, H-10), 10.83 (s, 1H, NH), 15.91 (s, 1H, 5 COOH). 6-Methyl-l-oxo-2-phenyl-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18n). From 17b and aniline (10 mol equivalents), as for 18g except that the product when filtered was washed 10 thoroughly with acetone. The acid was a lemon coloured solid (41%), mp >310 oC., which was used in the next step without further purification but can be recrystallized from 1,4-dioxane. 'H NMR (d 6 -DMSO): 8 2.73 (s, 3H, ArCH 3 ), 7.51-7.59 (m, 5H, Ph), 7.66 (t, J=7.6 Hz, 1H, H-8), 7.95 15 (d, J=6.9 Hz, 1H, H-7), 8.23 (d, J=8.3 Hz, 1H, H-9), 8.46 (s, IH, H-3), 9.51 (s, IH, H-10), 15.99 (br s, 1H, COOH). Anal. Calc. for C 20 H1 4

N

2 0 3 *0.4H 2 0: C, 71.2; H, 4.4; N, 8.3% Found: C, 71.3; H, 4.0; N, 8.25% The filtrate was taken to dryness at reduced 20 pressure, water was added, and the resultant yellow solid was filtered and washed with water to give N,N-Dimethyl-2 [2-(phenylamino)vinyl] -8-methylquinoline-3-carboxamide (32%), mp 143-146 'C (from light petroleum (bp 90-110 OC). 1 H NMR (d 6 -DMSO): 8 2.79 (s, 6H, ArCH 3 and NCH 3 ), 3.30 (s, 25 3H, NCH 3 ), 5.31 (d, J=8.5 Hz, 1H, HC=), 6.93 (t, J=7.3 Hz, 1H, H-4'), 7.17 (d, J=8.0 Hz, 2H, H-2',H-6'), 7.30-7.38 (m, 3H, H-6,H-3',H-5'), 7.52-7.62 (m, 2H, =CH,H-7), 7.69 (d, J=7.9 Hz, IH, H-5), 8.05 (s, 1H, H-4), 11.95 (d, J=ll.7 Hz, 1H, NH-exchanges with added D 2 0). "C NMR (d 6 -DMSO): 6 30 18.8 (ArCH 3 ), 34.3 (NCH 3 ), 38.2 (NCH 3 ), 94.5 (HC=), 115.0 (2 x CH, C-2',C-6'), 121.6 (CH, C-4'), 124.0 (C-4a), 125.0 (CH, C-6), 126.1 (CH, C-5), 129.5 (C, C-3), 129.9 (2 x CH, C-3',C-5'), 130.5 (CH, C-7), 133.5 (CH, C-4), 133.7 (C-8), 136.4 (=CH), 141.5 (C-l'), 145.3 (C-8a), 153.8 (C-2), 168.4 35 (CONMe 2 ). HRMS (EI): Calc. for C 21

H

21

N

3 0: 331.1686. Found: 331.1686.

WO 03/097642 PCT/AU03/00569 - 40 6-Methyl-1-oxo-2-(4-fluorophenyl) -1,2 dihydrobenzo [b][1,6]naphthyridine-4-carboxylic acid (18o). From 17b and 4-fluoroaniline (10 mol equivalents), as for 18n. The first filtered product was a 5 triethylamine salt of the target acid. This was dissolved in hot 5% sodium hydroxide, filtered, and the filtrate was cooled and acidified with concentrated hydrochloric acid to give the acid as a yellow solid (26%). 1H NMR (d 6 -DMSO) 6 2.75 (s, 3H, CH 3 ), 7.38-7.43 (m, 2-H), 7.60-7.70 (m, 3 10 H), 7.95 (d, 1H, J=6.0 Hz), 8.25 (d, 1H, J=8.4 Hz), 8.48 (s, 1-H), 9.53 (s, 1-H). Addition of water to the first filtrate gave a yellow-green solid which was recrystallized twice from methanol to give N,N-Dimethyl-2-[2-((4 15 fluorophenyl)amino)vinyl] -8-methylquinoline-3-carboxamide, mp 172-173 oC after changing form ca 100 aC. 1H NMR (d 6 DMSO): 8 2.78 (s, 3H, CH 3 ) 2.79 and 3.06 (2 x s, 6H,

N(CH

3 )2), 5.28 (d, 1H, J=8.5 Hz), 7.13-7.20 (m, 4H), 7.36 (t, 1H, J=7.4 Hz), 7.50 (dd, 1H, J=11.7, 8.5, Hz),7.61 (d, 20 1H, J=6.8 Hz), 7.70 (d, 1H, J=7.9 Hz), 8.06 (s, IH), 11.96 (d, 1H, J=11.7 Hz). 6-Methyl-1-oxo-2-[4-(4,4,5,5-tetramethyl [1,3,2]dioxaborolan-2-yl)phenyl] -1,2 dihydrobenzo[b][1, 6]naphthyridine-4-carboxylic acid (18p). 25 From 17b and 4-(4,4,5,5-tetramethyl-l,3,2 dioxaborolan-2-yl)aniline, as for 18g. A solution formed slowly and, after 16 h, the volume was reduced to ca 1/3 at reduced pressure, and the whole was cooled on ice. The solid which separated was filtered off, washed with cold 30 toluene and the recrystallized from toluene to give the acid as a yellow solid (20%), mp >300 oC. :H NMR (CDC1 3 ): 6 1.40 {s, 12H, 4 x CH 3 }, 2.87 (s, 3H, ArCH 3 ), 7.46 (d, J=8.0 Hz, 2H, ArH), 7.61 (t, J=7.6 Hz, 1H, H-8), 7.85 (d, J=6.8 Hz, 1H, H-7), 7.95-8.00 (m, 3H, H-9,ArH), 8.72 (s, 1H, H 35 3), 9.42 (s, 1H, H-10), 16.16 (s, IH, COOH). The filtrate from the reaction mixture (containing DMF, triethylanmine and toluene) was taken to dryness at WO 03/097642 PCT/AU03/00569 - 41 reduced pressure, and the residue was recrystallized from toluene to give the starting aniline as a yellow solid (45% recovery). 2-(3,4-Dimethoxyphenyl)-6-methyl-1-oxo-1,2 5 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acid (18q). A mixture of 4-aminoveratrole (0.12 g), 17b (0.20 g), dimethylformamide (5 mL) and triethylamine (1 mL) was stirred at room temperature for 16 h. Solid was present at all times and it was collected by filtration 10 and washed with a little cold dichloromethane to give 4 [(3,4-dimethoxyphenyl)aminomethylene]-6-methyl-4H pyrano[4,3-b]quinoline-1,3-dione (19) as a yellow solid (0.25 g, 90%), mp 280-281 °C. 1H NMR (d 6 -DMSO): 8 2.81 (s, 3H, CH 3 ), 3.79 (s, 3H, OCH 3 ), 3.87 (s, 3H, OCH 3 ), 7.06 (s, 15 2H), 7.21 (s, 1H), 7.52 (t, 1H, J=7.5 Hz, H-8), 7.83 (d, 1H, J=7.0 Hz), 8.02 (d, IH, J=7.9 Hz), 8.81 (s, 1H, J=13.2 Hz, CHN), 9.09 (s, 1H, H-10), 13.83 (d, 1H, J=13.2 Hz, NH). A mixture of 19 (0.23 g), 4-aminoveratrole (0.46 20 g), pyridine (15 mL) and triethylamine (1 mL) was heated under reflux for 8 h (dissolution occurred during the heating). The reaction mixture was allowed to stand at 10 OC overnight and the resulting precipitate was collected by filtration and washed with a little cold 25 acetone to give the product as a yellow solid (0.18 g, 78%), mp 297-298 OC (after forming needles >285 oC). 1H NMR (d 6 -DMSO): 6 2.73 (s, 3H, CH 3 ), 3.78 (s, 3H, OCH 3 ), 3.84 (s, 3H, OCH 3 ), 7.12 (s, 2H), 7.23 (s, 1H), 7.67 (t, IH, J=7.4 Hz, H-8), 7.95 (d, 1H, J=6.7 Hz), 8.23 (d, 1H, J=8.2 Hz), 30 8.45 (s, 1H), 9.50 (s, 1H, H-10), 15.98 (br s, 1H, COOH).

WO 03/097642 PCT/AU03/00569 - 42 Example 4 Preparation of 2,2'-[1,3 Propanediylbis[(methylimino)-2,1-ethanediyl]] bis[6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxylic 5 acid] (18aa). This is an example of the formation of a bis carboxylic acid precursor to a compound of formula II. N,N'-Bis-(2-aminoethyl)-N,N'-dimethylpropane 1,3-diamine (0.10 g) was added to a suspension of 17b 10 (0.30 g) in dimethylformamide (7.5 mL) and triethylamine (0.4 mL) and the resulting solution was stirred for 16 h at room temperature. The precipitate, which formed during the reaction, was collected by filtration and was stirred in ethyl acetate (10 mL) for 5 min. The solid was 15 collected by filtration to give the product as a yellow solid (0.15 g, 43%), mp 224-228 OC. 1H NMR (d 6 -DMSO): 6 1.45-1.49 (m, 2H), 2.13 (s, 6H, 2 x N-CH 3 ), 2.28-2.34 (m, 4H), 2.48-2.55 (m, 10H), 4.01-4.05 (m, 4H), 7.36 (t, 2H, J=7.4 Hz), 7.62 (d, 2H, J=6.5 Hz), 7.93 (d, 2H, J=7.7 Hz), 20 8.49 (s, 2H), 9.16 (s, 2H). Example 5 Preparation of N-[2-(Dimethylamino)ethyl]-2,6 dimethyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide 25 (20d). This is an example of general method A for amide formation according to Scheme 2d. Acid 18d (0.40 g) was heated under reflux in thionyl chloride (20 mL) for 30 min. The excess of thionyl 30 chloride was removed at reduced pressure and a solution of N,N-dimethylethylenediamine (0.5 mL) in dichloromethane (20 mL) was added to the residue. The resulting solution was stirred at room temperature for 16 h and the solution was washed with 10% sodium hydroxide and water (x 2). The 35 solvent was removed at reduced pressure to give the product as a yellow solid (0.48 g, 95%), mp 167-170 oC (from acetonitrile). IH NMR (CDC1 3 ): 5 2.28 (s, 6H, WO 03/097642 PCT/AU03/00569 - 43 N(CH 3

)

2 ), 2.61 (t, 2H, J=6.3 Hz), 2.82 (s, 3H, CH 3 ), 3.67 3.73 (m, 5H), 7.46 (t, 1H, J=7.6 Hz, H-8), 7.70 (d, 1H, J=6.9 Hz), 7.82 (d, 1H, J=8.2 Hz), 8.56 (s, 1H, H-3), 9.21 (s, 1H, H-10), 10.91 (br s, IH, CONH). 13C NMR (CDC1 3 ): 6 5 18.5 (C6-CH 3 ), 37.2 (N-CH 3 ), 37.6 (CH 2 ), 45.4 (N(CH 3

)

2 ), 58.8 (CH 2 ), 109.5 (C), 119.3 (C), 125.9 (C), 126.6 (CH), 127.3 (CH), 132.8 (CH), 135.9 (C), 139.8 (CH), 143.6 (CH), 148.2 (C), 148.8 (C), 162.8 (C), 164.5 (C). ESMS: m/z 339 (M+1). 10 Anal. Calc. for C 19

H

2 2

N

4 0 2 : C, 67.4; H, 6.6; N, 16.6. Found: C, 67.3; H, 6.6; N, 16.3. The following amides were made using a similar procedure. N- [2-(Dimethylamino)ethyl] -2-methyl-1-oxo-1,2 15 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20b) From acid 18b, and obtained as yellow plates (67%), mp 192-194 oC (from acetonitrile). IH NMR (CDC1 3 ): 6 2.40 (s, 6H, N(CH 3

)

2 ), 2.63 (t, 2H, J=6.1 Hz), 3.6-3.7 (m, 5-H), 7.60 (t, IH, J=7.1 Hz), 7.87 (t, 1H, J=7.5 Hz), 8.01 20 (d, 1H, J=8.2 Hz), 8.10 (d, IH, J=8.5 Hz), 8.58 (s, 1H), 9.31 (s, 1H, H-10), 11.30 (br s, 1H, CONH). 13C NMR (CDC1 3 ) : 6 36.8 (N-CH 3 ) , 37.0 (CH 2 ), 44.9 (N(CH 3

)

2 ), 57.7

(CH

2 ), 109.0 (C), 119.1 (C), 125.5 (C), 126.4 (CH), 128.0 (CH), 128.8 (CH), 132.5 (CH), 139.1 (CH), 143.2 (CH), 25 148.5 (C), 149.3 (C), 162.4 (C), 164.0 (C). Anal. Calc. for C 18

H

2 0

N

4 0 2 : C, 66.7; H, 6.2; N, 17.3. Found: C, 67.1; H, 5.7; N, 17.4. N-[2-(Dimethylamino)ethyl] -2-butyl-6-methyl-1 oxo-1,2-dihydrobenzo[b][1,6]naphthyridine-4-carboxamide 30 (20f) From acid 18f, and obtained as a yellow solid (89%), mp 127-128 oC [from light petroleum (bp 90-120 OC)]. 1H NMR (CDC1 3 ): 6 0.95 (t, 3H, J=7.3 Hz, CH 2

-CH

3 ), 1.41 (sextet, 2H, J=7.3 Hz, CH 2

-CH

3 ), 1.80 (quintet, 2H, 35 J=7.4 Hz, N2-CH 2

-CH

2 ), 2.29 (s, 6H, N(CH 3

)

2 ), 2.62 (t, 2H, J=6.5 Hz, CH 2

-N(CH

3

)

2 ), 2.85 (s, 3H, C6-CH 3 ), 3.72 (q, 2H, J=6.3 Hz, CONH-CH 2 ), 4.08 (t, 2H, J=7.4 Hz, N2-CH 2 ), 7.47 WO 03/097642 PCT/AU03/00569 - 44 (t, 1H, J=7.9 Hz, H-8), 7.71 (d, 1H, J=6.8 Hz), 7.85 (d, 1H, J=8.2 Hz), 8.59 (s, 1H, H-3), 9.27 (s, 1H, H-10), 10.97 (br s, 1H, CONH). 1C NMR (CDC1 3 ): 8 13.6 (CH 2

-CH

3 ), 18.5 (C6-CH 3 ), 19.8 (CH 2 ), 31.3 (CH 2 ), 37.6 (CH 2 ), 45.4 5 (N(CH 3

)

2 ), 49.4 (CH 2 ), 58.8 (CH 2 ), 109.5 (C), 119.5 (C), 125.9 (C), 126.5 (CH), 127.3 (CH), 132.8 (CH), 135.9 (C), 140.0 (CH), 143.0 (CH), 148.2 (C), 148.8 (C), 162.4 (C), 164.6 (C). Anal. Calc. for C 22

H

2 8

N

4 0 2 : C, 69.5; H, 7.4; N, 14.7. Found: 10 C, 69.5; H, 7.2; N, 14.7. N-[2-(Dimethylamino)ethyl] -2-(3 ethoxycarbonyl)propyl-6-methyl-1-oxo-1,2 dihydrobenzo[b][1, 6]naphthyridine-4-carboxamide (20j). From acid 18j, with a reflux time of 5 h, and 15 obtained as a yellow solid (74%), mp 130-132 0C (from toluene/light petroleum (bp 90-120 OC). 1 H NMR (CDC1 3 ): 8 1.23 (t, J=7.1 Hz, 3H, C02CH 2

CH

3 ), 2.15 (quintet, J=7.2 Hz, 2H, CH 2 CH2CH 2 ), 2.40 (t, J=7.2 Hz, 2H, NCH 2 CH2CH 2 ), 2.49 [s, 6H, N(CH 3

)

2 ], 2.84-2.89 (m, 5-H, ArCH 3 and CH 2

CH

2 NMe 2 ), 3.84 20 (q, J=6.2 Hz, 2H, CH 2

CH

2 NMe 2 ), 4.07-4.17 (m, 4H, CO2CH 2

CH

3 and NCH 2

CH

2

CH

2 ), 7.49 (t, J=7.6 Hz, 1H, H-8), 7.73 (d, J=6.9 Hz, 1H, H-7), 7.86 (d, J=8.2 Hz, 1H, H-9), 8.57 (s, 1H, H-3), 9.27 (s, 1H, H-10), 11.17 (br s, 1H, CONH). "C NMR (CDC1 3 ): 8 14.2 (C0 2

CH

2

CH

3 ), 18.6 (ArCH 3 ), 24.5 25 (CH 2

CH

2

CH

2 ) , 31.2 (NCH 2

CH

2

CH

2 ) , 36.8 (CH 2

CH

2 NMe 2 ), 44.7

[N(CH

3

)

2 ], 48.9 (NCH 2 CH2CH 2 ), 58.0 (CH 2

CH

2 NMe 2 ), 60.6 (C0 2

CH

2

CH

3 ), 109.5 (C-4), 119.4 (C-10a), 126.0 (C-9a), 126.8 (CH, C-8), 127.4 (CH, C-9), 133.1 (CH, C-7), 135.9 (C-6), 140.1 (CH, C-10), 142.9 (CH, C-3), 148.2 (C-5a), 30 148.6 (C-4a), 162.4 (C-1), 164.9 (CONH), 172.3 (CO 2 Et). Anal. Calc. for C 2 4

H

3 0

N

4 0 4

*H

2 0: C, 63.1; H, 7.1; N, 12.3. Found: C, 63.6; H, 7.0; N, 12.3.

WO 03/097642 PCT/AU03/00569 - 45 N-[2-(Dimethylamino)ethyl]-2-[2 (dimethylamino)ethyl]-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20k) From acid 18k, and obtained as a yellow solid 5 (84%), mp 141-143 oC [from light petroleum (bp 90-120 OC)]. H NMR (CDC1 3 ): 6 2.30 (s, 6H, N(CH 3

)

2 ), 2.38 (s, 6H,

N(CH

3

)

2 ), 2.67-2.76 (m, 4H), 2.86 (s, 3H, C6-CH 3 ), 3.78 (q, 2H, J=6.3 Hz, CH 2 ), 4.18 (t, 2H, J=6.6 Hz, CH 2 ), 7.49 (t, 1H, J=7.4 Hz, H-8), 7.73 (d, 1H, J=6.8 Hz), 7.87 (d, 1H, 10 J=8.2 Hz), 8.61 (s, 1H, H-3), 9.29 (s, 1H, H-10), 11.07 (br s, 1H, CONH). 13 C NMR (CDC1 3 ) : 6 18.6 (C6-CH 3 ), 37.1

(CH

2 ), 45.0 (N(CH 3

)

2 ), 45.6 (N(CH 3

)

2 ), 47.2 (CH 2 ), 57.7

(CH

2 ), 58.5 (CH 2 ), 109.3 (C), 119.5 (C), 126.0 (C), 126.6 (CH), 127.4 (CH), 133.0 (CH), 135.9 (C), 140.1 (CH), 143.4 15 (CH), 148.3 (C), 148.8 (C), 162.5 (C), 165.0 (C). Anal. Calc. for C 2 2

H

2 9

N

5 0s 2 00.25H 2 0: C, 66.1; H, 7.4; N, 17.5. Found: C, 66.2; H, 7.6; N, 17.4. N-[2-(Dimethylamino)ethyl]-2-[2 (dimethylamino)ethyl] -1-oxo-1,2 20 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (201) From acid 181, and obtained as a yellow solid (80%), mp 143-144 OC (from toluene). H NMR (CDC1 3 ): 6 2.29 (s, 6H, N(CH 3

)

2 ), 2.42 (s, 6H, N(CH 3

)

2 ), 2.65-2.71 (m, 4H), 3.70 (q, 2H, J=6.0 Hz, CH 2 ), 4.17 (t, 2H, J=6.5 Hz, CH 2 ), 25 7.60 (dd, 1H, J=8.4 Hz, 7.4 Hz), 7.88 (t, 1H, J=7.3 Hz), 8.02 (d, 1H, J=8.2 Hz), 8.12 (d, IH, J=8.6), 8.60 (s, IH, H-3), 9.32 (s, 1H, H-10), 11.32 (br s, 1H, CONH). Anal. Calc. for C 2 1

H

27

N

5 0 2 : C, 66.1; H, 7.1; N, 18.4. Found: C, 65.7; H, 7.1; N, 17.9. 30 (S)-N-[2-(Dimethylamino)ethyl]-6-methyl-1-oxo-2 (1-phenylethyl) -1,2-dihydrobenzo[b][1,6]naphthyridine-4 carboxamide (20m) From acid 18m, and obtained as a yellow semi solid (86%). 'H NMR (CDC1 3 ): 6 1.05 (d, 3H, J=7.2 Hz, CH 35 CH 3 ), 2.27 (s, 6H, N(CH 3

)

2 ), 2.59 (t, 2H, J=6.5 Hz, CH 2 N(CH 3

)

2 ), 2.85 (s, 3H, C6-CH 3 ), 3.67 (q, 2H, J=6.7 Hz,

CONH-CH

2 ), 6.45 (q, 1H, J=7.1 Hz, N2-CH), 7.27-7.41 (m, WO 03/097642 PCT/AU03/00569 - 46 5H), 7.45 (dd, 1H, J=8.1, 7.3 Hz, H-8), 7.69 (d, 1H, J=7.0 Hz), 7.84 (d, 1H, J=8.2 Hz), 8.62 (s, IH, H-3), 9.30 (s, 1H, H-10), 10.96 (br s, 1H, CONH). 3C NMR (CDC1 3 ): 6 18.5

(CH

3 ), 19.1 (CH 3 ), 37.4 (CH 2 ), 45.4 (N(CH 3

)

2 ), 53.4 (CH), 5 58.7 (CH2), 110.0 (C), 119.4 (C), 126.0 (C), 126.5 (CH), 127.2 (CH), 127.3 (CH), 128.2 (CH), 128.9 (CH), 132.8 (CH), 135.9 (C), 139.56 (CH), 139.61 (CH), 140.3 (CH), 148.2 (C), 148.4 (C), 162.4 (C), 164.6 (C). HRMS (LSI): Calc. for C 2 6

H

2 9

N

4 0 2 [(M+H) ]: 429.2292. Found: 429.2298. 10 N-[2-(Dimethylamino)ethyl] -6-methyl-2-phenyl-1 oxo-1,2-dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20n). From acid 18n, and obtained as pale yellow needles (55%), mp 199-201 'C (from methanol). H NMR 15 (CDC1 3 ): 6 2.35 [s, 6H, N(CH 3

)

2 ], 2.70 (t, J=6.4 Hz, 2H,

CH

2

CH

2 NMe 2 ), 2.88 (s, 3H, ArCH 3 ), 3.77 (q, J=6.1 Hz, 2H,

CH

2

CH

2 NMe 2 ), 7.43-7.55 (m, 6H, H-8, Ph), 7.75 (d, J=6.9 Hz, 1H, H-7), 7.88 (d, J=8.1 Hz, 1H, H-9), 8.71 (s, 1H, H-3), 9.32 (s, 1H, H-10), 11.06 (br s, IH, CONH). 13C NMR (CDC1 3 ): 20 6 18.6 (ArCH 3 ), 37.5 (CH 2

CH

2 NMe 2 ), 45.3 [N(CH 3

)

2 ], 58.6

(CH

2

CH

2 NMe 2 ), 110.0 (C-4), 119.9 (C-10a), 126.2 (C-9a), 126.7 (2 x CH, Ph), 126.8 (CH, C-8), 127.4 (CH, C-9), 128.8 (CH, C-4'), 129.5 (2 x CH, Ph), 133.1 (CH, C-7), 136.1 (C-6), 140.1 (C-I'), 140.5 (CH, C-10), 143.4 (CH, C 25 3), 148.4 (C-5a), 148.9 (C-4a), 162.2 (C-1), 164.6 (CONH). Anal. Calc. for C 2 4

H

2 4

N

4 0 2 : C, 72.0; H, 6.0; N, 14.0. Found: C, 71.8; H, 6.0; N, 13.75. N-[2-(Dimethylamino)ethyl]-2-(4-fluorophenyl)-6 methyl-1-oxo--1,2-dihydrobenzo[b] [1,6]naphthyridine-4 30 carboxamide (20o) From acid 18o, and obtained as pale yellow needles (88%), mp 219-220 oC (from acetonitrile). IH NMR (CDC1 3 ): 6 2.45 (s, 6H, N(CH3) 2 ), 2.82 (t, 2H, J=6.3 Hz,

CH

2

-N(CH

3

)

2 ), 2.87 (s, 3H, C6-CH 3 ), 3.83 (q, 2H, J=6.4 Hz, 35 CONH-CH 2 ), 7.18-7.25 (m, 2H), 7.42-7.55 (m, 3H), 7.76 (d, 1H, J=7.0 Hz), 7.89 (d, 1H, J=8.1 Hz), 8.65 (s, 1H, H-3), 9.30 (s, 1H, H-10), 11.14 (br s, 1H, CONH). 13C NMR WO 03/097642 PCT/AU03/00569 - 47 (CDC1 3 ): 5 18.4 (C6-CH 3 ), 35.7 (CH 2 ), 43.7 (N(CH 3

)

2 ), 56.9

(CH

2 ), 109.1 (C), 116.15 (d, J=23.1 Hz,C3',5'-H), 119.2 (C), 125.8 (C), 126.8 (CH), 127.0 (CH), 128.16 (d, J=8.5 Hz,C2',6'-H), 133.2 (CH), 135.5 (C), 140.3 (CH), 142.8 5 (CH), 147.8 (C), 148.0 (C), 161.8 (C), 162.0 (d, J=244.5 Hz, C4'), 164.8 (C). Anal. Calc. for C 2 4

H

2 3

FN

4 0 2 : C, 68.9; H, 5.5; N, 13.4. Found: C, 68.9; H, 5.6; N, 13.4. N- [2-(Dimethylamino)ethyl]-2-(3,4 10 dimethoxyphenyl) -6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20q) From acid 18q, and obtained as a yellow solid (92%), mp 199-201 oC (from toluene). 1H NMR (CDC1 3 ): 6 2.35 (s, 6H, N(CH 3

)

2 ), 2.70 (t, 2H, J=6.3 Hz, CH 2

-N(CH

3 )2), 2.89 15 (s, 3H, C6-CH 3 ), 3.77 (q, 2H, J=6.4 Hz, CONH-CH 2 ), 3.90 (s, 3H, OCH 3 ), 3.94 (s, 3H, OCH 3 ), 6.96-6.99 (m, 3H), 7.52 (t, 1H, J=7.5 Hz, H-8), 7.76 (d, 1H, J=7.0 Hz), 7.89 (d, 1H, J=8.1 Hz), 8.71 (s, 1H, H-3), 9.34 (s, 1H, H-10), 11.07 (br s, 1H, CONH) . 13C NMR (CDC1 3 ) : 6 18.6 (C6-CH 3 ), 37.3 20 (CH 2 ), 45.2 [N(CH 3

)

2 ], 56.1 (2 x OCH 3 ), 58.5 (CH 2 ), 109.7 (C), 110.6 (CH), 111.2 (CH), 118.9 (CH), 119.8 (C), 126.1 (C), 126.9 (CH), 127.4 (CH), 133.0 (C), 133.2 (CH), 136.0 (C), 140.5 (CH), 143.8 (CH), 148.4 (C), 148.6 (C), 149.3 (C), 149.4 (C), 162.5 (C), 164.7 (C). 25 Anal. Calc. for C 26

H

2 8

N

4 0 4 .0.5H 2 0: C, 66.5; H, 6.2; N, 11.9. Found: C, 66.2; H, 6.4; N, 11.3. N-[2-(Dimethylamino)ethyl] -6-chloro-7-methoxy-2 methyl-1-oxo-1,2-dihydro-benzo[b][1,6]naphthyridine-4 carboxamide (20x) 30 From acid 18w, with a reflux time of 5 h, and obtained as a brown solid (73%), mp 209-212 oC (from acetonitrile). 'H NMR (CDC1 3 ): 6 2.32 [s, 6H, N(CH 3

)

2 ], 2.69 (t, J=6.8 Hz, 2H, CH 2

CH

2 NMe 2 ), 3.69-3.77 (m, 5H, CH 2

CH

2 NMe 2 and NCH 3 ), 4.14 (s, 3H, ArOCH 3 ), 7.45 (d, J=9.2 Hz, 1H), 35 7.97 (d, J=9.2 Hz, IH), 8.63 (s, IH, H-3), 9.24 (s, IH, H 10), 11.17 (br s, 1H, CONH). 13C NMR (CDC1 3 ): 8 37.2 (CH 3 ), 37.9 (CH 2 ), 45.6 [N(CH 3

)

2 ], 57.0 (CH 3 ), 58.9 (CH 2 ), 109.4 WO 03/097642 PCT/AU03/00569 - 48 (C), 114.4 (CH), 116.6 (C), 118.2 (C), 121.9 (C), 129.1 (CH), 139.9 (CH), 144.5 (CH), 146.1 (C), 150.5 (C), 158.3 (C), 162.6 (C), 164.3 (C). Anal. Calc. for C1 9

H

21 C1N 4 0 3 C, 58.7; H, 5.4; N, 14.4. 5 Found: C, 58.3; H, 5.4; N, 14.3. N-[2-(Dimethylamino)ethyl]-7-methyl-6-oxo-6,7 dihydropyrido[2,3-b] [1,6]naphthyridine-9-carboxamide (20y) From acid 18y, with a reflux time of 1.5 h (complete dissolution did not occur). The crude amide was 10 added to a short silica column. This was first eluted with dichloromethane/methanol (19:1) and then with dichloromethane/methanol (1:1) which furnished the amide as a yellow solid (17%), mp 218-221 °C (from acetonitrile). 1H NMR (CDC1 3 ): 6 2.41 [s, 6H, N(CH 3

)

2 ], 2.74 (t, J=6.6 Hz, 15 2H, CH 2

CH

2 NMe 2 ), 3.70 (s, 3H, ArCH 3 ), 3.76 (q, J=6.3 Hz, 2H,

CH

2

CH

2 NMe 2 ), 7.57 (dd, J=8.2, 4.2 Hz, IH, H-3), 8.40 (dd, J=8.3, 2.0 Hz, 1H, H-4), 8.68 (s, 1H, H-8), 9.27 (dd, J=4.2, 2.0, 1H, H-2), 9.36 (s, 1H, H-5), 10.95 (br s, 1H, CONH). 13C NMR (CDC1 3 ) : 6 37.0 (NCH 3 ), 37.3 (CH 2

CH

2 NMe 2 ), 20 45.1 [N(CH 3

)

2 ], 58.2 (CH 2

CH

2 NMe2), 109.1 (C-9), 120.1 (C), 120.4 (C), 122.1 (CH, C-3), 138.3 (CH, C-4), 141.4 (CH, C 5), 145.0 (CH, C-8), 152.1 (C-9a), 155.2 (C-10a), 157.2 (CH, C-2), 161.9 (C-6), 163.7 (CONH). Anal. Calc. for C1 7 Hi 9 gNsO.

2

-H

2 0: C, 59.5; H, 6.2; N, 20.4. 25 Found: C, 59.6; H, 5.7; N, 20.3%. N-[2-(Dimethylamino)ethyl]-2-methyl-1-oxo-1,2 dihydropyrido[3,4-b]quinoxaline-4-carboxamide (20z) From acid 18z, with a reflux time of 45 min., and obtained as golden flakes (47%), mp 215-218 OC (after 30 forming needles >109 oC) (from acetonitrile). 1 H NMR (CDC1 3 ): 6 2.41 [s, 6H, N(CH 3

)

2 ], 2.65 (t, J=6.1 Hz, 2H,

CH

2

CH

2 NMe 2 ), 3.67 (q, J=5.7 Hz, 2H, CH 2

CH

2 NMe 2 ), 3.75 (s, 3H, NCH 3 ), 7.80-7.86 (m, 1H), 7.88-7.95 (m, 1H), 8.08 (d, J=8.3 Hz, 1H), 8.38 (d, J=8.1 Hz, 1H), 8.63 (s, IH), 10.61 35 (br s, 1H). 13C NMR (CDC1 3 ): 6 37.1 (CH 2 ), 37.5 (CH 3 ), 44.9

[N(CH

3

)

2 ], 57.5 (CH 2 ), 108.0 (C), 127.9 (CH), 130.4 (CH), WO 03/097642 PCT/AU03/00569 - 49 130.5 (CH), 133.3 (CH), 136.7 (C), 141.4 (C), 142.2 (C), 143.5 (CH), 144.9 (C), 160.9 (C), 163.2 (C). The hydrochloride had mp 218-221 oC (from ethanol). 5 2,2'-[1,3-Propanediylbis[(methylimino)-2,1 ethanediyl ] ]bis[N-(2-(dimethylamino)ethyl) -6-methyl-1-oxo 1,2-dihydrobenzo[b][1, 6]naphthyridine-4-carboxamide (20aa) From acid 18aa, and obtained as a yellow solid (75%), mp 97-102 oC [from light petroleum (bp 90-120 OC)]. 10 H NMR (CDC1 3 ): 5 1.55-1.63 (m, 2H), 2.23 (s, 6-H), 2.42 2.84 [m, 30-H, incl. 2.50 (s, 12H), 2.72 (s, 6-H)], 3.78 (q, 4H, J=6.2 Hz, CH 2 ), 4.08 (t, 4H, J=5.9 Hz, CH2), 7.31 (t, 2H, J=7.8 Hz), 7.53 (d, 2H, J=6.8 Hz), 7.71 (d, 2H, J=8.2 Hz), 8.51 (s, 2H), 9.11 (s, 2H), 10.96 (br s, 2H, 15 2xCONH). 3 C NMR (CDC1 3 ): 6 18.5 (CH 3 ), 24.8 (CH 2 ), 36.9

(CH

2 ), 42.1 (NCH 3 ), 44.9 (N(CH 3

)

2 ), 46.8 (CH 2 ), 54.9 (CH 2 ), 56.5 (CH 2 ), 58.2 (CH 2 ), 108.6 (C), 119.2 (C), 125.6 (C), 126.4 (CH), 127.1 (CH), 132.6 (CH), 135.6 (C), 139.7 (CH), 143.8 (CH), 147.8 (C), 148.4 (C), 162.1 (C), 164.8 (C). 20 ESMS: m/z 402.3 [(M+2)/2, 100%], 803.5 (M+1, 50%). Anal. Calc. for C 4 5

H

5 8

N,

0 0 4 -1.5H 2 0: C, 65.1; H, 7.4; N, 16.9. Found: C, 65.5; H, 7.2; N, 16.3. (S)-N- [1- ((Dimethylamino)carbonyl)ethyl]-2,6 dimethyl-1-oxo-1,2-dihydrobenzo[b][1,6]naphthyridine-4 25 carboxamide (20bb) From acid 18d, with a reflux time of 45 min. To the residue from removal of the thionyl chloride was added triethylamine (2.2 mol equivalents) in dichloromethane followed by (S)-2-amino-N,N-dimethylpropionamide (1.1 mol 30 equivalents) in dichloromethane. The standard reaction and work up gave the amide (88%) as yellow needles, mp 268-274 0 C (from acetonitrile). H NMR (d 6 -DMSO, 100 oC): 6 1.42 (d, J=6.8 Hz, 3H, CHCH 3 ), 2.81 (s, 3H, ArCH 3 ), 2.99 [br s, 6H,

N(CH

3

)

2 ], 3.62 (s, 3H, NCH 3 ), 5.21 (quintet, J=7.1 Hz, 1H, 35 CHCH 3 ), 7.53 (t, J=7.6 Hz, 1H, H-8), 7.79 (d, J=6.8 Hz, 1H, H-7), 8.03 (d, J=8.2 Hz, IH, H-9), 8.55 (s, 1H, H-3), 9.26 (s, IH, H-10), 10.84 (br d, J=7.7 Hz, 1H, CONH). 13C NMR WO 03/097642 PCT/AU03/00569 - 50 (d 6 -DMSO, 100 oC): 6 18.2 (ArCH 3 ), 18.4 (CHCH 3 ), 36.1 [br s,

N(CH

3

)

2 ], 36.5 (NCH 3 ), 44.6 (CHCH 3 ), 108.6 (C-4), 119.1 (C 10a), 125.8 (C-9a), 126.6 (CH, C-8), 127.5 (CH, C-9), 133.0 (CH, C-7), 135.7 (C-6), 139.6 (CH, C-10), 144.7 (CH, 5 C-3), 147.8 (C-5a), 148.7 (C-4a), 162.0 (C-1), 162.8 (CONH), 172.1 (CONMe 2 ) . The hydrochloride had mp 277-280 OC (yellow needles from ethanol). (S)-N-[2-(l-Dimethylamino)propyl] -2,6-dimethyl-l 10 oxo-1,2-dihydrobenzo[b][1,6] naphthyridine-4-carboxamide (20cc) From acid 18d, with a reflux time of 45 min. To a suspension in dichloromethane of the residue from removal of the thionyl chloride was added a solution of (S)-N',N 15 dimethylpropane-1,2-diamine hydrochloride (2.2 mol equivalents) and triethylamine (2.2 mol equivalents) in dichloromethane. The standard reaction and work up gave the amide (38%) as a light brown solid, mp 205-209 'C (from acetonitrile). 1 H NMR (d 6 -DMSO, 100 oC): 8 1.46 (d, J=6.6 20 Hz, 3H, CHCH 3 ), 2.81 (s, 3H, ArCH 3 ), 2.84 [s, 6H, N(CH 3

)

2 ], 3.31 (d, J=6.8 Hz, 2H, CH 2 NMe 2 ), 3.64 (s, 3H, NCH 3 ), 4.64 (quintet, J=6.9 Hz, 1H, CHCH 3 ), 7.57 (t, J=7.6 Hz, 1H, H 8), 7.84 (d, J=6.0 Hz, IH, H-7), 8.08 (d, J=8.1 Hz, 1H, H 9), 8.60 (s, 1H, H-3), 9.31 (s, IH, H-10), 10.75 (br s, 25 1H, CONH). 13C NMR (d 6 -DMSO, 100 OC): 6 18.5 (ArCH 3 ), 19.6

(CHCH

3 ), 36.6 (NCH 3 ), 43.4 [N(CH 3

)

2 ], 62.4 (CH 2 NMe 2 ), 108.4 (C-4), 119.2 (C-10a), 125.9 (C-9a), 126.8 (CH, C-8), 127.7 (CH, C-9), 133.3 (CH, C-7), 135.2 (C-6), 139.8 (CH, C-10), 144.9 (CH, C-3), 147.7 (C-5a), 148.7 (C-4a), 162.0 (C-1), 30 164.2 (CONH). CHCH 3 was not observed. The hydrochloride had mp 251-254 oC after forming needles >200 oC (mustard powder from acetonitrile).

WO 03/097642 PCT/AU03/00569 - 51 N-[(2-Dimethylamino)ethyl]-6-methyl-1-oxo-1,2 dihydrobenzo[b][1 , 6]naphthyridine-4-carboxamide perchlorate salt (20c) From the ammonium salt of 18c, and obtained in 5 crude form as a yellow solid (69%). The perchlorate salt was prepared in ethanol, recrystallized from 'moist' methanol, and obtained as a brown solid, mp 218-219 OC (explosive decomposition >250 OC). H NMR (d 4 -MeOD) : 8 2.91 (s, 9-H, C6-CH 3

+N(CH

3

)

2 ), 3.19 (t, 2H, J=6.2 Hz, CH 2 ), 3.45 10 (t, 2H, J=6.2 Hz), 6.96-7.00 (m, 2H), 7.21 (br s, 1H), 7.37 (br s, 1H), 7.67 (br s, 1H). ESMS: m/z 325 (M+l, 100%), 163 [(M+2)/2, 12%]. Anal. Calc. for C 1 8

H

2 0

N

4 0 2 *HClO 4 *2H 2 0: C, 46.9; H, 5.5; N, 12.2. Found: C, 46.6; H, 5.2; N, 12.6. 15 Example 6 Preparation of N-[2-(Dimethylamino)ethyl]-2 ethyl-6-methyl-1-oxo-1,2-dihydrobenzo[b][1,6] naphthyridine-4-carboxamide (20e). This is an example of general method B for amide 20 formation according to Scheme 2d. A mixture of acid 18e (0.23 g, 0.81 mmol)and l,1'-carbonyldiimidazole (CDI) (0.66 g, 4.07 mmol) in 1,4 dioxane (15 mL) was heated under reflux for 24 h, during which time dissolution occurred. The solvent was removed 25 at reduced pressure and to the residue was added, N,N dimethylethylenediamine (1 mL) in dichloromethane (25 mL). The solution was then stirred for 16 h at room temperature. More dichloromethane was added and the solution was washed with 10% sodium hydroxide (x l)and 30 water (x 3). The organic fraction was dried over magnesium sulphate and the solvent was removed at reduced pressure to give the amide 20e as a bright yellow solid (0.26 g, 91%), mp 157-158 oC (from acetonitrile) .

1 H NMR (CDC1 3 ) : 8 1.37 (t, J=7.2 Hz, 3H, CH 2

CH

3 ), 2.23 [s, 6H, N(CH 3

)

2 ], 2.54 35 (t, J=6.5 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.66 (s, 3H, ArCH 3 ), 3.63 (q, J=6.2 Hz, 2H, CH 2

CH

2 NMe 2 ), 4.05 (q, J=7.2 Hz, 2H, CH 2

CH

3 ), 7.32 (dd, J=8.0, 7.2 Hz, 1H, H-8), 7.55 (d, J=6.9 Hz, 1H, WO 03/097642 PCT/AU03/00569 - 52 H-7), 7.65 (d, J=8.2 Hz, H-1, H-9), 8.48 (s, 1H, H-3), 8.99 (s, 1H, H-10), 10.73 (br t, J=5.1 Hz, CONH). C NMR (CDC1 3 ): 6 14.4 (CH 2

CH

3 ), 18.3 (ArCH 3 ), 37.5 (CH 2

CH

2 NMe 2 ), 44.6 (CH 2

CH

3 ), 45.3 [N(CH 3

)

2 ], 58.7 (CH 2

CH

2 NMe 2 ), 109.5 (C 5 4), 119.2 (C-10a), 125.5 (C-9a), 126.2 (CH, C-8), 127.1 (CH, C-9), 132.4 (CH, C-7), 135.6 (C-6), 139.4 (CH, C-10), 142.4 (CH, C-3), 147.7 (C-5a), 148.4 (C-4a), 161.9 (C-1), 164.3 (CONH). Anal. Calc. for C 2 0

H

2 4

N

4 0 2 C, 68.2; H, 6.9; N, 15.9. Found: 10 C, 68.5; H, 6.8; N, 16.1. The following compounds were prepared in a similar manner: N-[2-(Dimethylamino)ethyl]-6-methyl-1-oxo-2 (2,2,2-trifluoroethyl)-1,2 15 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20g). From acid 18g, and obtained as a yellow solid (83%), mp 227-230 oC (from acetonitrile). 'H NMR (CDC1 3 ): 6 2.30 [s, 6H, N(CH 3 )2], 2.63 (t, J=6.4 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.85 (s, 3H, ArCH 3 ), 3.72 (q, J=6.1 Hz, 2H, CH 2

CH

2 NMe 2 ), 20 4.73 (q, J=8.4 Hz, 2H, CH 2

CF

3 ), 7.51 (t, J=7.6 Hz, 1H, H 8), 7.74 (d, J=6.9 Hz, IH, H-7), 7.85 (d, J=8.2 Hz, H-1, H-9), 8.58 (s, 1H, H-3), 9.27 (s, 1H, H-10), 10.84 (br s, 1H, CONH). 13C NMR (CDC1 3 ) : 6 18.4 (ArCH 3 ), 37.8

(CH

2

CH

2 NMe 2 ), 45.4 [N(CH 3

)

2 ], 48.3 (q, J=35.3 Hz, CH 2

CF

3 ), 25 58.8 (CH 2

CH

2 NMe 2 ), 111.3 (C-4), 119.1 (C-10a), 123.4 (q, J=280 Hz, CH 2

CF

3 ), 126.3 (C-9a), 127.2 (CH, C-8), 127.4 (CH, C-9), 133.4 (CH, C-7), 136.3 (C-6), 140.6 (CH, C-10), 142.1 (CH, C-3), 148.6 (C-5a), 148.7 (C-4a), 162.3 (C-1), 164.0 (CONH). 30 Anal. Calc. for C 2 0

H

2 1

F

3

N

4 0 2 : C, 59.1; H, 5.2; N, 13.8. Found: C, 59.4; H, 5.4; N, 13.9. N-[2-(Dimethylamino)ethyl] -2 (ethoxycarbonylmethyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6] -naphthyridine-4-carboxamide (20i). 35 From acid 18i with a reflux time of 48 h and a recharge with an equal amount of CDI after 24 h, and obtained as a yellow solid (79%), mp 214-215 oC (from WO 03/097642 PCT/AU03/00569 - 53 acetonitrile). 1H NMR (CDC1 3 ): 8 1.28 (t, J=7.2 Hz, 3H,

CO

2

CH

2

CH

3 ), 2.29 [s, 6H, N(CH 3

)

2 ], 2.62 (t, J=6.4 Hz, 2H,

CH

2

CH

2 NMe 2 ), 2.87 (s, 3H, ArCH 3 ), 3.72 (q, J=5.9 Hz,

CH

2

CH

2 NMe 2 ), 4.25 (q, J=7.1 Hz, CO 2

CH

2

CH

3 ), 4.78 (s, 2H, 5 CH 2

CO

2 Et), 7.51 (t, J=7.6 Hz, 1H, H-8), 7.74 (d, J=6.9 Hz, 1H, H-7), 7.87 (d, J=8.2 Hz, 1H, H-9), 8.53 (s, 1H, H-3), 9.28 (s, 1H, H-10), 10.96 (br t, J=4.8 Hz, 1H, CONH). 13C NMR (CDC1 3 ): 6 13.7 (CO 2

CH

2

CH

3 ), 18.1 (ArCH 3 ), 37.3

(CH

2

CH

2 NMe 2 ), 45.1 [N(CH 3

)

2 ], 50.1 (CH 2

CO

2 Et), 58.4 10 (CH 2

CH

2 NMe 2 ), 61.6 (CO 2

CH

2

CH

3 ) , 109.8 (C-4), 118.7 (C-10a), 125.5 (C-9a), 126.4 (CH, C-8), 126.9 (CH, C-9), 132.6 (CH, C-7), 135.6 (C-6), 139.6 (CH, C-10), 142.7 (CH, C-3), 147.8 (C-5a), 148.4 (C-4a), 162.0 (C-1), 163.9 (CONH), 166.9 (CO 2 Et). 15 Anal. Calc. for C 2 2

H

2 6N 4 0 4 : C, 64.4; H, 6.4; N, 13.65. Found: C, 64.5; H, 6.4; N, 13.9. N-[2-(Dimethylamino)ethyl]-2- (4-boronophenyl)-6 methyl-1-oxo-1,2-dihydrobenzo [b][1, 6]naphthyridine-4 carboxamide ( 2 0p). 20 From acid 18p, carried out under nitrogen, with a reflux time of 48 h and a recharge with an equal amount of CDI after 24 h. When the amination reaction was complete, the volatiles were removed at reduced pressure with heat (-0.3 mmHg, 100 oC, 20 min) and residual N,N 25 dimethylethylenediamine was removed by azeotropic distillation with toluene (x 3). The residue was then boiled in toluene and, while hot, decanted from a brown oil. The toluene was removed at reduced pressure, and the residue was recrystallized from acetonitrile to give the 30 intermediate N-[2-(dimethylamino)ethyl] -6-methyl-l-oxo-2 [4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2 yl)phenyl] 1, 2-dihydrobenzo[b] [1, 6]naphthyridine-4 carboxamide as a yellow solid (74%), mp 139-141 oC. H NMR (CDC1 3 ): 6 1.34 {s, 12H, 4 X CH 3 }, 2.31 [s, 6H, N(CH 3

)

2 ], 35 2.64 (t, J=6.5 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.81 (s, 3H, ArCH 3 ), 3.72 (q, J=6.1 Hz, 2H, CH 2

CH

2 NMe 2 ), 7.42-7.48 (m, 3H, H 8,2',6'), 7.69 (d, J=6.9 Hz, 1H, H-7), 7.80 (d, J=8.1 Hz, WO 03/097642 PCT/AU03/00569 - 54 1H, H-9), 7.95 (d, J=8.3 Hz, 2H, H-3',5'), 8.68 (s, IH, H 3), 9.23 (s, 1H, H-10), 10.98 (br t, J=5.4 Hz, 1H, CONH). 1C NMR (CDC1 3 ): 8 18.2 (ArCH 3 ), 24.5 (4 x CH 3 ), 37.2

(CH

2

CH

2 NMe 2 ), 45.0 [N(CH 3

)

2 ], 58.3 (CH 2

CH

2 NMe 2 ), 83.8 (2 x 5 C), 109.7 (C-4), 119.4 (C-10a), 125.5 (2 x CH, C-2',6'), 125.7 (C-9a), 126.4 (CH, C-8), 127.0 (CH, C-9), 132.7 (CH, C-7), 135.6 (2 X CH, C-3',5'), 140.1 (CH, C-10), 142.0 (C 1'), 142.8 (CH, C-3), 147.8 (C-5a), 148.4 (C-4a), 161.7 (C-1), 164.1 (CONH). C-6 and C-4' were not observed. 10 Water (5 mL) was added to a solution of this compound (0.12 g, 0.23 mmol) in methanol (5 mL), and the whole was heated at reflux for 30 min. The volume was then reduced to ca -2 mL) at reduced pressure, water was added, and the solid was filtered, washed with water and recrystallized 15 from ethanol to give the boronic acid 20p as a yellow solid (0.05 g, 49%), mp 242-244 oC. :H NMR (d 6 -DMSO): 6 2.23 [s, 6H, N(CH 3

)

2 ], 2.56 (t, J=5.8 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.82 (s, 3H, ArCH 3 ), 3.59 (q, J=5.7 Hz, 2H, CH 2

CH

2 NMe 2 ), 7.52 (d, J=8.0 Hz, 2H, H-3',5'), 7.59 (t, J=7.5 Hz, 1H, H-8), 7.86 20 (d, J=6.7 Hz, 1H, H-7), 7.95 (d, J=8.0 Hz, 2H, H-2',6'), 8.14 (d, J=8.0 Hz, 1H, H-9). 8.23 [s, 2H, B(OH) 2 ], 8.43 (s, 1H, H-3), 9.39 (s, 1H, H-10), 10.76 (br t, J=4.8 Hz, 1H, CONH). 13C NMR (d 6 -DMSO): 6 17.8 (ArCH 3 ), 37.1 (CH 2

CH

2 NMe 2 ), 45.1 [N(CH 3

)

2 ], 58.5 (CH 2

CH

2 NMe 2 ), 109.2 (C-4), 119.5 (C 25 10a), 125.7 (C-9a), 125.8 (C-2',6'), 126.8 (CH, C-8), 127.6 (CH, C-9), 133.2 (CH, C-7), 135.1 (C-3',5'), 135.3 (C-6), 140.1 (CH, C-10), 141.8 (C-1'), 143.2 (CH, C-3), 147.4 (C 5a), 148.4 (C-4a), 161.3 (C-1), 163.2 (CONH). C-4' was not observed. 30 Anal. Calc. for C 24

H

2 5

BN

4 0 4 : C, 64.9; H, 5.9; N, 12.5. Found: C, 64.4; H, 5.9; N, 12.5. N-[2-(Dimethylamino)ethyl] -2-(3,4 dimethoxybenzyl)-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20r). 35 From acid 18r with a reflux time of 48 h and a recharge with an equal amount of CDI after 24 h, and obtained as a yellow solid (85%), mp 213-214 oC (from WO 03/097642 PCT/AU03/00569 - 55 acetonitrile). 1 H NMR (CDC1 3 ): 8 2.22 [S, 6H, N(CH 3

)

2 ], 2.52 (t, J=6.4 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.63 (s, 3H, ArCH 3 ), 3.62 (q, J=6.1 Hz, 2H, CH 2

CH

2 NMe 2 ), 3.75 (s, 3H, OCH 3 ), 3.78 (s, 3H,

OCH

3 ), 5.09 (s, 2H, CH 2 Ph), 6.74 (d, J=8.5 Hz, 1H, H-5'), 5 6.90-6.92 (m, 2H, H-2',H-6'), 7.27 (t, J=7.6 Hz, 1H, H-8), 7.49 (d, J=6.8 Hz, 1H, H-7), 7.61 (d, J=8.1 Hz, 1H, H-9), 8.55 (s, 1H, H-3), 8.99 (s, 1H, H-10), 10.70 (br t, J=5.3 Hz, 1H, CONH). 13C NMR (CDC1 3 ): 5 18.2 (ArCH 3 ), 37.4

(CH

2

CH

2 NMe 2 ), 45.2 [N(CH 3

)

2 ], 51.9 (CH 2 Ph), 55.6 (OCH 3 ), 55.7 10 (OCH 3 ), 58.6 (CH 2

CH

2 NMe 2 ), 109.6 (C-4), 111.1 (CH, C-5'), 111.5 (CH, C-6') , 119.1 (C-10a), 120.9 (CH, C-2'), 125.4 (C 9a), 126.2 (CH, C-8), 126.9 (CH, C-9), 128.2 (C-I'), 132.4 (CH, C-7), 135.5 (C-6), 139.5 (CH, C-10), 142.3 (CH, C-3), 147.6 (C-5a), 148.2 (C-4a), 148.9 (C-4'), 149.1 (C-3'), 15 162.1 (C-1), 164.1 (CONH). Anal. Calc. for C 2 7

H

30

N

4 0 4 C, 68.3; H, 6.4; N, 11.8. Found: C, 68.5; H, 6.6; N, 11.8. N-[2-(Dimethylamino)ethyl] -2-[2-(3,4-dimethoxy phenyl)ethyl] -6-methyl-1-oxo-1,2 20 dihydrobenzo[b][1,6]naphthyridine-4-carboxamide (20s). From acid 18s with a reflux time of 48 h and a recharge with an equal amount of CDI after 24 h, and obtained as a yellow solid (81%), mp 113-114 'C (from acetonitrile). H NMR (CDC1 3 ): 6 2.26 [s, 6H, N(CH 3

)

2 ], 2.58 25 (t, J=6.5 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.77 (s, 3H, ArCH 3 ), 3.01 (m, 2H, CH 2

CH

2 Ph), 3.67 (q, J=6.1 Hz, 2H, CH 2

CH

2 NMe 2 ), 3.79 (s, 6H, 2 X OCH 3 ), 4.22 (m, 2H, CH 2

CH

2 Ph), 6.72-6.75 (m, 3H, H 2', H-5' and H-6'), 7.41 (t, J=7.6 Hz, 1H, H-8), 7.64 (d, J=6.8 Hz, 1H, H-7), 7.77 (d, J=8.2 Hz, 1H, H-9), 8.51 (s, 30 1H, H-3), 9.15 (s, 1H, H-10), 10.83 (br t, J=5.3 Hz, 1H, CONH). 13C NMR (CDC1 3 ): 5 18.4 (ArCH 3 ), 34.9 (CH 2

CH

2 Ph), 37.5

(CH

2

CH

2 NMe 2 ), 45.4 [N(CH 3

)

2 ], 51.2 (CH 2

CH

2 Ph), 55.8 (2 x

OCH

3 ), 58.7 (CH 2

CH

2 NMe 2 ), 109.5 (C-4), 111.4 (CH, Ar), 111.9 35 (CH, Ar), 119.3 (C-10a), 120.7 (CH, Ar), 125.7 (C-9a), 126.5 (CH, C-8), 127.2 (CH, C-9), 129.8 (C, Ar), 132.7 (CH, C-7), 135.8 (C-6), 139.7 (CH, C-10), 142.7 (CH, C-3), WO 03/097642 PCT/AU03/00569 - 56 147.8 (C, Ar), 148.0 (C-5a), 148.6 (C-4a), 149.0 (C, Ar), 162.2 (C-1), 164.3 (CONH). Anal. Calc. for C 2 8

H

3 2

N

4 0 4 : C, 68.8; H, 6.6; N, 11.5. Found: C, 68.7; H, 6.9; N, 11.5. 5 N-[2- (Dimethylamino)ethyl] -6-methyl-2-(pyridin-2 yl)methyl-1-oxo-1,2-dihydrobenzo[b][1,61] -naphthyridine-4 carboxamide (20t). From acid 18t, with a reflux time of 48 h and a recharge with an equal amount of CDI after 24 h, and 10 obtained as a bright yellow solid (75%), mp 183-184 oC, (from acetonitrile). 1H NMR (CDC1 3 ): 6 2.25 [s, 6H,

N(CH

3

)

2 ], 2.57 (t, J=6.4 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.67 (s, 3H, ArCH 3 ), 3.66 (q, J=6.1 Hz, CH 2 , CH 2

CH

2 NMe 2 ), 5.30 (s, 2H,

CH

2 Pyr), 7.10 (dd, J=7.0, 5.2 Hz, 1H, H-5'), 7.26-7.33 (m, 15 2H, H-3',H-8), 7.52-7.59 (m, 2H, H-4',H-7), 7.64 (d, J=8.2 Hz, 1H, H-9), 8.46 (d, J=4.4 Hz, 1H, H-6'), 8.66 (s, IH, H 3), 9.02 (s, 1H, H-10), 10.79 (br t, J=5.1 Hz, IH, CONH). 13 C NMR (CDC1 3 ) : 6 18.3 (ArCH 3 ), 37.3 (CH 2

CH

2 NMe 2 ), 45.2

[N(CH

3

)

2 ], 53.7 (CH 2 Pyr), 58.6 (CH 2

CH

2 NMe 2 ), 109.6 (C-4), 20 119.1 (C-10a), 122.1 (CH, C-3'), 122.7 (CH, C-5'), 125.5 (C 9a), 126.4 (CH, C-8), 127.0 (CH, C-9), 132.6 (CH, C-7), 135.6 (C-6), 136.7 (CH, C-4'), 139.6 (CH, C-10), 143.4 (CH, C-3), 147.7 (C-5a), 148.5 (C-4a), 149.6 (CH, C-6'), 155.0 (C-2'), 162.2 (C-1), 164.3 (CONH). 25 Anal. Calc. for C 2 4

H

2 5

N

5 0 2 : C, 69.4; H, 6.1; N, 16.9. Found: C, 69.1; H, 6.2; N, 16.8. N-[2-(Dimethylamino)ethyl] -6-methyl-1-oxo-2-[3 (2-oxopyrrolidin-1-yl)propyl] -1,2-dihydrobenzo[b][1,6] naphthyridine-4-carboxamide (20u). 30 From acid 18u with a reflux time of 24 h, and obtained as a yellow solid (89%), mp 170-171 'C, (from acetonitrile). 1 H NMR (CDC1 3 ): 8 1.96-2.06 (m, 4H), 2.25 [s, 6H, N(CH 3

)

2 ], 2.35 (t, J=8.1 Hz, 2H), 2.58 (t, J=6.5 Hz, 2H), 2.75 (s, 3H, ArCH 3 ), 3.38 (m, 4H), 3.66 (q, J=5.9 Hz, 35 2H), 4.02 (m, 2H), 7.41 (dd, J=7.3, 7.9 Hz, IH, H-8), 7.64 (d, J=6.9 Hz, 1H, H-7), 7.76 (d, J=8.1 Hz, 1H, H-9), 8.52 (s, 1H, H-3), 9.13 (s, 1H, H-10), 10.85 (br t, J=3.5 Hz, WO 03/097642 PCT/AU03/00569 - 57 1H, CONH). 13C NMR (CDC1 3 ): 8 17.6 (CH 2 ), 18.0 (ArCH 3 ), 26.6

(CH

2 ), 30.5 (CH 2 ), 37.2 (CH 2 ), 39.5 (CH 2 ), 45.0 [N(CH 3

)

2 ], 46.6 (CH 2 ), 47.1 (CH 2 ), 58.3 (CH 2 ), 109.3 (C-4), 118.8 (C 10a), 125.3 (C-9a), 126.2 (CH, C-8), 126.8 (CH, C-9), 5 132.8 (CH, C-7), 135.4 (C-6), 139.2 (CH, C-10), 142.2 (CH, C-3), 147.6 (C-5a), 148.1 (C-4a), 161.8 (C-1), 163.9 (CONH), 174.8 (C-2'). Anal. Calc. for C 2 5

H

3 1

N

5 0 3 : C, 66.8; H, 7.0; N, 15.6. Found: C, 66.3; H, 7.1; N, 15.7. 10 N-[2-(Dimethylamino)ethyl] -7-methoxy-2-methyl-1 oxo-1,2-dihydro-benzo[b][1, 6]naphthyridine-4-carboxamide (20w) From acid 18w, with a reflux time of 96 h and a recharge with an equal amount of CDI after 48 h, and 15 obtained as a yellow solid (80%), mp 213-215 OC (from acetonitrile). 1H NMR (CDC1 3 ): 8 2.50 [s, 6H, N(CH 3

)

2 ], 2.78 (t, J=6.0 Hz, 2H, CH 2

CH

2 NMe 2 ), 3.67 (s, 3H, NCH 3 ), 3.77 (q, J=5.8 Hz, 2H, CH 2

CH

2 NMe 2 ), 4.02 (s, 3H, ArOCH 3 ), 7.22 (dd, J=9.1, 2.2 Hz, 1H, H-8), 7.43 (d, J=2.2 Hz, 1H, H-6), 7.85 20 (d, J=9.1 Hz, 1H, H-9), 8.53 (s, 1H, H-3), 9.14 (s, 1H, H 10), 11.36 (br s, IH, CONH). 13C NMR (CDC13): 6 36.8

(CH

2

CH

2 NMe 2 and NCH 3 ), 45.0 [N(CH 3

)

2 ], 55.6 (ArOCH 3 ), 57.7

(CH

2

CH

2 NMe 2 ), 105.3 (CH, C-6), 108.8 (C-4), 117.3 (C-10a), 120.9 (CH, C-8), 121.4 (C-9a), 130.0 (CH, C-9), 138.3 (CH, 25 C-10), 143.1 (CH, C-3), 149.7 (C-4a), 150.9 (C-5a), 162.5 (C-1), 163.4 (C-7), 164.4 (CONH). Anal. Calc. for C 19

H

22

N

4 0 3 C, 64.4; H, 6.3; N, 15.8. Found: C, 64.4; H, 6.5; N, 15.6. N-[2-(Dimethylamino)ethyl] -2-[((2 30 (dimethylamino)ethyl)amino)carbonyloxy] ethyl-6-methyl-1 oxo-1,2-dihydrobenzo[b][ 1,6]naphthyridine-4-carboxamide (21a) From acid 18h, with a reflux time of 48 h, and obtained as a yellow solid (63%), mp 75-76 oC (from toluene 35 X 3). 'H NMR (CDC1 3 ) : 6 2.17 [s, 6H, N(CH 3

)

2 ], 2.28 [s, 6H,

N(CH

3

)

2 ], 2.34 (t, J=6.0 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.60 (t, J=6.5 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.81 (s, 3H, ArCH 3 ), 3.19 (q, J=5.8 Hz, WO 03/097642 PCT/AU03/00569 - 58 2H, CH2CH 2 NMe 2 ), 3.69 (q, J=6.2 Hz, 2H, CH 2

CH

2 NMe 2 ), 4.32 (m, 2H, CH 2

CH

2 OCO), 4.41 (m, 2H, CH 2

CH

2 OCO), 5.82 (br s, 1H, CONH), 7.47 (t, J=7.6 Hz, 1H, H-8), 7.70 (d, J=6.8 Hz, 1H, H-7), 7.83 (d, J=8.1 Hz, 1H, H-9), 8.58 (s, 1H, H-3), 9.23 5 (s, 1H, H-10), 10.99 (br s, 1H, 4-position CONH). 13C NMR (CDC1 3 ): 6 18.2 (ArCH 3 ), 37.3 (CH 2

CH

2 NMe 2 ), 38.1

(CH

2

CH

2 NMe 2 ), 44.8 [N(CH 3

)

2 ], 45.1 [N(CH 3

)

2 ], 48.8

(CH

2

CH

2 0CO) , 58.0 (CH 2

CH

2 NMe 2 ) , 58.5 (CH 2

CH

2 NMe 2 ) , 61.9

(CH

2

CH

2 0CO), 109.0 (C-4), 119.0 (C-10a), 125.6 (C-9a), 10 126.3 (CH, C-8), 127.0 (CH, C-9), 132.6 (CH, C-7), 135.5 (C-6), 139.7 (CH, C-10), 143.4 (CH, C-3), 147.9 (C-5a), 148.4 (C-4a), 155.6 (CONH), 162.1 (C-1), 164.4 (CONH). HRMS (EI): Calc. for C 25

H

34

N

6 0 4 [M+] : 482.2643. Found: 482.2626. 15 N-[2-(Dimethylamino)ethyl] -2- [N-(((2 (dimethylamino)ethyl)amino)carbonyl) -1H-indol-3-yl] ethyl 6-methyl-1-oxo-1, 2-dihydrobenzo[b] [1, 6]naphthyridine-4 carboxamide (21b) From acid 18v with a reflux time of 48 h and a 20 recharge with an equal amount of CDI after 24 h, and obtained as a yellow solid (62%), mp 182-185 0C, (from acetonitrile). 1 H NMR (CDC1 3 ) : 6 2.27 [s, 12H, 2 X N(CH 3

)

2 ], 2.52-2.61 (m, 4H, 2 x CH 2

CH

2 NMe 2 ), 2.77 (s, 3H, ArCH 3 ), 3.18 (t, J=7.7 Hz, 2H, CH 2

CH

2 Indole), 3.50 (q, J=5.4 Hz, 2H, 1' 25 CH 2

CH

2 NMe 2 ), 3.68 (q, J=6.0 Hz, 2H, 4-CH 2

CH

2 NMe 2 ), 4.32 (t, J=7.7 Hz, 2H, CH 2

CH

2 Indole), 6.60 (br t, J=4.1 Hz, 1H, 1' CONH), 7.18 (t, J=7.4 Hz, 1H, H-5'), 7.27 (t, J=7.8 Hz, 1H, H-6'), 7.39-7.44 (m, 2H, H-2',H-8), 7.65 (m, 2H, H-4',H-7), 7.77 (d, J=8.2 Hz, 1H, H-9), 8.09 (d, J=8.2 Hz, 1H, H-7'), 30 8.55 (s, 1H, H-3), 9.17 (s, 1H, H-10), 10.87 (br t, J=5.2 Hz, 1H, 4- CONH). 13C NMR (CDC1 3 ): 6 18.4 (ArCH3), 24.8

(CH

2

CH

2 Indole) , 37.6 (4-CH 2

CH

2 NMe 2 ), 37.9 (1'-CH 2

CH

2 NMe 2 ), 45.0 [N(CH 3

)

2 ], 45.4 [N(CH 3

)

2 ], 49.7 (CH 2

CH

2 Indole), 57.7 (1'-CH 2

CH

2 NMe 2 ), 58.7 (4-CH 2

CH

2 NMe 2 ), 109.5 (C-4), 114.4 (CH, 35 C-7'), 115.7 (1'-CONH), 118.8 (CH, C-4'), 119.3 (C-10a), 121.98 (CH, C-2'), 122.03 (CH, C-5'), 124.4 (CH, C-6'), 125.8 (C-9a), 126.5 (CH, C-8), 127.2 (CH, C-9), 129.5 (C, WO 03/097642 PCT/AU03/00569 - 59 C-3a'), 132.8 (CH, C-7), 135.6 (C-7a'), 135.8 (C-6), 139.7 (CH, C-10), 142.7 (CH, C-3), 148.0 (C-5a), 148.5 (C-4a), 152.0 (C-3') , 162.2 (C-1), 164.4 (4-CONH). Anal. Calc. for C 3 3

H

3 9

N

7 0 3 .0.5H 2 0: C, 67.1; H, 6.8; N, 16.6. 5 Found: C, 67.2; H, 6.7; N, 16.6. Example 7 Preparation of N-[2-(Dimethylamino)ethyl]-2 hydroxyethyl-6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6]-naphthyridine-4-carboxamide 10 (20h). This is an example of hydrolytic modification of a 2-substituent within a 4-carboxamide according to Scheme 2e. To the carbamate 21a (0.55 g, 1.49 mmol) in 15 ethanol (25 mL) was added 10% sodium hydroxide (20 mL), and the whole was heated under reflux for 1 h, then cooled on ice, adjusted to pH -8 with concentrated hydrochloric acid, and evaporated to dryness at reduced pressure. The residual solid was boiled in acetonitrile, the mixture was 20 filtered while hot, and the filtrate was taken to dryness at reduced pressure to give an orange solid (0.26 g). This solid was dissolved in a little warm ethanol and added to a bed of silica. This was eluted with a little ethanol, then with ethanol/triethylamine (25:1). The 25 ethanol/triethylamine eluate was taken to dryness at reduced pressure. The residual solid (0.17 g) was dissolved in a little chloroform, filtered through a bed of basic alumina, washed with chloroform, and then eluted with methanol. The methanol eluate was evaporated to 30 dryness under reduced pressure to give a yellow solid (0.12 g) which was recrystallized twice from dichloromethane to give the amide 20h as a yellow solid (0.10 g, 24%), mp 174-180 oC. 1 H NMR (CDC1 3 ): 8 2.27 [s, 6H,

N(CH

3

)

2 ], 2.43 (s, 3H, ArCH 3 ), 2.54 (t, J=6.4 Hz, 2H, 35 CH 2

CH

2 NMe 2 ), 3.55 (q, J=6.0 Hz, 2H, CH 2

CH

2 NMe 2 ), 4.05 (m, 2H, CH 2

CH

2 OH), 4.20 (m, 2H, CH 2

CH

2 OH), 7.34 (t, J=7.5 Hz, 1H, H-8), 7.50 (d, J=6.7 Hz, 1H, H-7), 7.63 (d, J=8.1 Hz, WO 03/097642 PCT/AU03/00569 - 60 1H, H-9), 8.56 (s, 1H, H-3), 8.96 (s, 1H, H-10), 10.67 (br t, J=5.0 Hz, 1H, CONH). 13C NMR (CDC1 3 ): 8 17.9 (ArCH 3 ), 37.0 (CH 2

CH

2 NMe 2 ), 45.0 [N(CH 3

)

2 ], 52.3 (CH 2

CH

2 OH), 58.1

(CH

2

CH

2 NMe 2 ), 60.2 (CH 2

CH

2 OH), 108.2 (C-4), 118.7 (C-10a), 5 125.1 (C-9a), 126.1 (CH, C-8), 126.8 (CH, C-9), 132.3 (CH, C-7), 135.3 (C-6), 139.0 (CH, C-10), 143.9 (CH, C-3), 147.3 (C-5a), 147.9 (C-4a), 162.4 (C-1), 164.3 (CONH). HRMS (LSI): Calc. for C2 0

H

2 5

N

4 0 3 [(M+H) ]: 369.1928. Found: 369.1940. 10 The filtrate from the chloroform washings was evaporated to dryness under reduced pressure to give 2-(2 (dimethylamino)ethyl)-4- [ (2 (dimethylamino) ethylamino ) methylene ] -6-methyl-4H benzo[b][1,6]naphthyridine-1,3-dione (22) as a yellow 15 solid (0.04 g, 9%), mp 202-206 0C. IH NMR (CDC1 3 ): 6 2.28 [s, 6H, N(CH 3

)

2 ], 2.34 [s, 6H, N(CH 3

)

2 ], 2.58-2.72 (m, 4H, 2 x CH 2

CH

2 NMe 2 ), 2.85 (s, 3H, ArCH 3 ), 3.66 (q, J=6.0 Hz, 2H,

CH

2

CH

2 NMe 2 ), 4.26 (t, J=7.2 Hz, 2H, CH 2

CH

2 NMe 2 ) , 7.34 (t, J=7.6 Hz, 1H, H-8), 7.60 (d, J=7.0 Hz, 1H, H-7), 7.72 (d, 20 J=8.0 Hz, 1H, H-9), 8.44 (d, J=13.6 Hz, 1H, =CH), 8.95 (s, 1H, H-10), 11.76 (br m, IH, NH). 13C NMR (CDC1 3 ): 8 18.3 (ArCH 3 ), 37.6 (CH 2

CH

2 NMe 2 ), 45.1 [N(CH 3

)

2 ], 45.4 [N(CH 3

)

2 ], 48.2 (CH 2

CH

2 NMe 2 ), 56.8 (CH 2

CH

2 NMe 2 ) , 59.1 (CH 2

CH

2 NMe 2 ), 94.2 (C), 116.5 (C), 124.3 (C-9a), 124.5 (CH, C-8), 127.2 (CH, 25 C-9), 131.9 (CH, C-7), 134.0 (C-6), 138.8 (CH, C-10), 147.8 (C-5a), 152.0 (C-4a), 155.8 (CH, =CH), 163.7 (C-1), 165.4 (C-3). ESMS: m/z 396.2 (M+1), 198.7 [(M+2)/2]. The following modification was carried out in a similar manner (Scheme 2f): 30 N-[2-(Dimethylamino)ethyl]-2-[2-(1H-Indol-3 yl) ethyl] -6-methyl-1-oxo-1,2 dihydrobenzo[b][1,6] naphthyridine-4-carboxamide (20v) To a hot solution of the bisamide 21b (0.58 g, 1.00 mmol) in ethanol (10 mL) was added 10% sodium 35 hydroxide (10 mL), and the whole was heated at reflux for 40 min. The reaction mixture was then evaporated to dryness under reduced pressure, water was added and the WO 03/097642 PCT/AU03/00569 - 61 solid was filtered and washed with water to give 20v as a gold solid (0.42 g, 90%), mp 105-107 oC (from acetonitrile). 1H NMR (CDC1 3 ): 6 2.31 [s, 6H, N(CH 3

)

2 ], 2.64 (t, J=6.4 Hz, 2H, CH 2

CH

2 NMe 2 ), 2.82 (s, 3H, ArCH 3 ), 3.21 (t, 5 J=7.7 Hz, 2H, CH 2

CH

2 Indole), 3.73 (q, J=6.1 Hz, 2H,

CH

2

CH

2 NMe 2 ), 4.30 (t, J=7.8 Hz, 2H, CH 2

CH

2 Indole), 6.99 (d, J=2.0 Hz, 1H, H-2'), 7.06-7.14 (m, 2H, H-5',H-6'), 7.30 (d, J=7.7 Hz, 1H, H-7'), 7.46 (t, J=7.6 Hz, 1H, H-8), 7.66-7.70 (m, 2H, H-4',H-7), 7.82 (d, J=8.2 Hz, IH, H-9), 8.51 (s, 10 1H, H-3), 8.62 (s, 1H, H-I'), 9.24 (s, 1H, H-10), 10.98 (br t, J=5.3 Hz, 1H, CONH). 13C NMR (CDC1 3 ): 8 18.2 (ArCH 3 ), 24.8 (CH 2

CH

2 Indole), 37.2 (CH 2

CH

2 NMe 2 ), 45.0 [N(CH 3

)

2 ], 50.2

(CH

2

CH

2 Indole), 58.4 (CH 2

CH

2 NMe 2 ), 109.0 (C), 110.9 (CH, C 7'), 111.1 (C), 118.2 (CH, C-4'), 119.2 (CH, C-5'), 121.7 15 (CH, C-6'), 122.0 (CH, C-2'), 125.6 (C-9a), 126.2 (CH, C-8), 126.8 (C-3a'), 127.0 (CH, C-9), 132.5 (CH, C-7), 135.5 (C 6), 136.0 (C-7a'), 139.5 (CH, C-3), 142.7 (CH, C-10), 147.8 (C-5a), 148.4 (C-4a), 162.1 (C-1), 164.4 (CONH). Indole C 3' was not observed. 20 HRMS (El): Calc. for C 28

H

29

N

5 0 2 [M]: 467.2323. Found: 467.2314. Example 8: Anti-Tumour Activity In Vitro The compounds identified in Table 1 were 25 evaluated for growth inhibitory properties, measured as IC50 values, against murine P388 leukemia cells, Lewis lung carcinoma cells (LLTC), and human Jurkat leukemia cells (JLC), together with their amsacrine- and doxorubicin resistant derivatives (JLA and JLD respectively), which 30 were obtained and cultured as previously described (Finlay et al, 1990; 1994). Growth inhibition assays were performed by culturing cells in microculture plates (150 pl per well) as follows: P388: 4.5 X 103 cells/well; 3 days 35 LLTC: 1 X 103 cells/well; 4 days Jurkat lines: 3.75 X 103 cells/well; 4 days Cell growth was determined by [3H]-thymidine WO 03/097642 PCT/AU03/00569 - 62 uptake (P388) (Marshall et al, 1992) or by the sulphorhodamine assay (Skehan et al, 1990). Independent assays were performed in duplicate, using doxorubicin, etoposide, camptothecin and DACA as reference compounds. 5 The results are summarized in Table 2. Table 2 Anti-Tumour Activity In Vitro of l-oxo-1,2 dihydrobenzo[b] [1,6]naphthyridine-4-carboxamides and prior 10 art reference compounds 9 10 1 Z8 N 7 N 4 3 6 5 CONHR 15

IC

50 a (nM) ICso ratio b Cpd Y Z P388c LLd JLe s A/C D/C ZOb Me H 14 20c0 H 6-Me 11 10 26 1.9 2.6 20d f Me 6-Me 2.1 1.7 6.7 5.6 7.9 20e f Et 6-Me 3.7 20 ff Bu 6-Me 14 15 51 2.3 3.0 20g f

CH

2

CF

3 6-Me 61 20h f

(CH

2 ) 2 0H 6-Me 4.8 20i CH 2

CO

2 Et 6-Me 12 20j (CH 2

)

3

CO

2 Et 6-Me 10 20k f

(CH

2

)

2 NMe 2 6-Me 6.8 3.7 8.5 0.7 0.9 201f (CH 2

)

2 NMe 2 H 54 50 123 0.8 1.1 20m f CH(Me)Ph-(s) 6-Me 590 161 1070 0.8 1.1 20n Ph 6-Me 12 20o f

C

6

H

4 F-4 6-Me 10 20p C 6

H

4 B (OH) 2 -4 6-Me 7.9 20q f

C

6

H

3 [3,4-(MeO) 2 ] 6-Me 12 4.4 9.4 1.3 1.5 Or, CH 2

C

6

H

3 [3,4- (MeO) 2] 6-Me 160 20s' (CH 2 ) 2C 6

H

3 [3,4- (MeO) 2 ] 6-Me 810 WO 03/097642 PCT/AU03/00569 - 63 -Ot" CH 2 (2-pyridinyl) 6-Me 13 -Ou N 6-Me 50 H a 0v 6-Me 210 2 0w Me 7-MeO 36 20x' Me 6-C1-7-MeO 60 a Oy Me 6-aza 19 0aoz f Me 10-aza 1000 Sla (CH 2 ) 20CONH (CH 2 2 NIMe 2 6-Me <125 H OI, NMe,' a 1b' C N ' 6-Me 140

(CH,

) aOaaf' (CH 2

)

2 NMe(CH 2

)

3 NMe(CH 2 ) 2 6-Me 22 4.7 1.0 0.3 0.4 a0bbh Me 6-Me 17000 o20cc i Me 6-Me 5 doxorubicin 15 22 9.6 4.4 12.7 etoposide 25 180 160 13.3 90.3 camptothecin 13 33 5.6 2.0 1.4 2 (DACA) 71 190 580 1.9 2.3 Footnotes a IC 50 ; concentration of drug to reduce cell number to 50% of control cultures. 5 b IC 5 0 values for the Jurkat lines JLA and JLD, respectively, relative to JLc-see text C Murine P388 leukemia. d Murine Lewis lung carcinoma. e Human Jurkat leukemia. 10 R = CH 2

CH

2 NMe 2 g A bis compound, an example of Formula II h R = (S)-CH(Me)CONMe 2 S R = (S)-CH(Me)CH 2 NMe 2 15 The JLA line is resistant to the DNA intercalator amsacrine and similar agents because of a WO 03/097642 PCT/AU03/00569 - 64 reduced level of topo II enzyme. The JLD line is resistant to doxorubicin, primarily by virtue of altered levels of topo II, but probably also via additional mechanisms. The ratios of the IC 50 values of a drug in 5 the parent line compared with one of the sublines (IC50[JLA]/IC 50 [JLC] and (IC50[JLD]/IC50[JLC]) therefore provide some indication of the mechanism of cytotoxicity. Classical topo II inhibitors such as amsacrine, doxorubicin and etoposide have large ratios (10-90 fold), 10 whereas topo I inhibitors such as camptothecin and mixed topo I/II inhibitors such as DACA (4) have ratios of only about 2-fold. Values of these ratios of less than about 1.5-2 therefore suggest cytotoxicity by a non-topo II mediated mechanism. 15 Example 9: Anti-Tumour Activity In Vivo Compound 20d was evaluated against murine colon 38 tumours implanted subcutaneously in C57BL/6 mice. This advanced colon 38 tumour model is fairly refractory to 20 standard clinical topo II agents, as well as to anti metabolites and alkylating agents. In vivo models using the colon 38 tumour model have been shown to be the best predictors for clinical utility to date (Goldin, A, 1980). It therefore represents a good test for in vivo activity. 25 Colon 38 tumours were grown subcutaneously from 1 mm 3 fragments implanted in one flank of C57/Bl mice (anaesthetised with pentobarbitone 90 mg/kg). When tumours reached a diameter of approximately 4 mm (7-8 days), mice were divided into control and drug treatment 30 groups (5 mice/group), with similar average tumour volumes in each group. Drugs were administered as solutions of the hydrochloride salts in distilled water, and were injected in a volume of 0.01 mL/g body weight. Mice treated with the prior art compounds Doxorubicin, 35 Daunorubicin, Amsacrine, Mitoxantrone, DACA, Etoposide or Irinotecan were used as positive controls. The mice were monitored closely, and tumour diameters were measured with WO 03/097642 PCT/AU03/00569 - 65 callipers three times a week. Tumour volumes were calculated.as 0.52xa2xb, where a and b are the minor and major tumour axes, and data plotted on a semilogarithmic plot as mean tumour volumes (± SEM) versus time after 5 treatment. The results for 20d are shown in Figure 3 and summarized for all compounds in Table 3. The growth delay was calculated as the time taken for tumours to reach a mean volume four-fold higher than their pre-treatment volume. 10 Table 3. In Vivo Activity of l-Oxo-l,2 dihydrobenzo[b][1,6]naphthyridine-4-carboxamides and Reference Compounds Against Subcutaneous Colon 38 Tumours in Mice 15 Drug Dose Schedule Growth delay Cures mg/kg/day a days b 20c 30c s.d. 14 0/5 20d 8.9c s.d. >20 4/4 20d 5.9 s.d. >20 10/10 20d 3.9 s.d. >20 4/4 20d 2.6 s.d. 10 0/5 20f 13.3c s.d. >20 5/5 20f 5.9 s.d. 18 0/5 20k 5.9 s.d. 14 0/5 20q 3.9C s.d. >20 4/5 20q 2.6 s.d. 16 0/5 Doxorubicin 2.6 q4dx3 8 0/5 Daunorubici 3.9 q4dx3 0 0/5 n Amsacrine 13.3 q4dx3 2 0/5 Mitoxantron 3.9 q4dx3 2 0/5 e DACA 200 q7dx2 13 0/5 Etoposide 45 q4dx3 1.5 0/5 Irinotecan 65 q4dx3 7 0/5 WO 03/097642 PCT/AU03/00569 - 66 a q2dx2 = every 2 days x 2; q4dx3 = every 4 days x 3; q7dx2 = every 7 days X 2; s.d. = single dose. b Number of mice with no measurable tumour 20 days after commencement of treatment/total number of mice. 5 c Highest dose which did not produce evidence of toxicity. It is evident from Table 3 that of the prior art compounds tested, only Doxorubicin, Irinotecan, and DACA 10 resulted in a significant delay in growth of the Colon 38 tumour, and none resulted in long term survival. However, single doses of compound 20d resulted in a growth delay of at least 20 days at all but the lowest dosage level. In these cases, the mice treated were long term survivors, 15 with no tumour present at 60 days after the end of treatment. Theoretically this is long enough for a single surviving cell to repopulate the tumour, so these mice would almost certainly have had normal life spans if held for a sufficiently long time. 20 Example 10: Anti-Tumour Activity In Vivo Compound 20d was evaluated against a human melanoma xenograft and demonstrated a tumour growth delay of 30 days when given in two doses of 5.9 mg/kg, 25 administered 7 days apart. The NZM4 melanoma cell line (Marshall et al., 1994) was grown in culture and 1 x 107 cells were implanted subcutaneously in one flank of athymic (nude) mice. When tumours reached a diameter of approximately 6 mm (15 days), mice were divided into 30 control and drug treatment groups (5 mice/group), with similar average tumour volumes in each group. Drugs were administered as solutions of the hydrochloride salts in distilled water and were injected in a volume of 0.01 mL/g body weight. The mice were monitored closely, and tumour 35 diameters were measured with callipers twice per week. Tumour volumes were calculated as 0.52xa 2 Xb, where a and b are the minor and major tumour axes, and data plotted on a WO 03/097642 PCT/AU03/00569 - 67 semilogarithmic plot as mean tumour volumes (± SEM) versus time after treatment. The results for 20d are shown in Figure 4. The growth delay was calculated as the time taken for tumours to reach a mean volume three-fold higher 5 than their pre-treatment volume. DISCUSSION The results show that the compounds of the present invention have cytotoxic activity against animal 10 and human tumour cell lines, and are active against transplanted tumours in mice. Thus they have potential utility as anti-cancer drugs. It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be 15 made to the invention as shown in the specific embodiments, without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive. 20 References cited herein are listed on the following pages, and are incorporated herein by this reference.

WO 03/097642 PCT/AU03/00569 - 68 REFERENCES Ames, D.E. and Dodds, W.D. J. Chem. Soc. Perkin Trans. 1, 1972, 705. 5 Antonini, I.; Cola, D.; Polucci, P.; Bontemps-Gracz, M.; Borowski, E.; Martelli, S. J. Med. Chem. 1995, 38, 3282. 10 Asbury, R.; Blessing, J. A.; Reid, G. C.; McGuire. W. P. Am. J. Clin. Oncol. 1998, 21, 406. Asherson, J.L. and Young, D.W. J. Chem. Soc., Chem. Comm., 1977, 916. 15 Atwell, G.J.; Rewcastle, G.W.; Baguley, B.C. and Denny, W.A. J. Med. Chem. 1987, 30, 664. 20 Baguley, B.C.; Zhuang, L. and Marshall, E.M. Cancer Chemother. Pharmacol. 1995, 36, 244. Cholody, W.M.; Hernandez, L.; Hassner, L.; Scudiero, D.A.; Djurickovic, D.B. and Michejda, C.J. 25 J. Med. Chem. 1995, 38, 3043. Cholody, W. M.; Horowska, B.; Paradziej-Lukowicz, J.; Martelli, S.; Konopa, J. J. Med. Chem., 1996, 39, 1028. 30 Deady, L. W.; Kaye, A.J.; Finlay, G.J.; Baguley, B.C. and Denny, W.A. J. Med. Chem., 1997, 40, 2040. 35 Deady, L.W.; Desneves, J.; Kaye, A.J.; Finlay, G.J.; Baguley, B.C. and Denny, W.A. Bioorg. Med. Chem., 2000, 8, 977.

WO 03/097642 PCT/AU03/00569 - 69 Deady, L.W. and Rodemann, T. J. Heterocycl. Chem., 2001, 38, 1185. 5 Denny, W.A. Proceedings of the 13th Annual Conference of the Royal Australian Chemical Institute Medicinal and Agricultural Division 1996, Abstract 5-1. 10 Diab, S. G.; Baker, S. D.; Joshi, A.; Burris, H. A.; Cobb, P. W.; Villalona-Calero, M. A.; Eckhardt, S. G.; Weiss, G. R.; Rodriguez, G. I.; Drengler, R.; Kraynak M.; Hammond, L.; Finizio, M.; Von Hoff, D. D.; Rowinsky, E. K. Clin. Cancer Res., 1999, 5, 299. 15 Finlay, G. J.; Baguley, B. C.; Snow, K. and Judd, W. J. Natl. Cancer Inst. 1990, 82, 662. Finlay, G. J.; Holdaway, K. M.; Baguley, B.C. 20 Oncol. Res. 1994, 6, 33-37. Gabriel, S. Ber. Dtsch. Chem. Ges., 1885, 18, 2445, 3470. 25 Gamage, S.A.; Spicer, J.A.; Finlay, G.J.; Stewart, A.J.; Charlton, P.; Baguley, B.C. and Denny, W.A. J. Med. Chem., 2001, 44, 1407. Goldin, A.; Venditti, J.M; Progress report on the 30 screening program at the division of cancer treatment, NCI. Cancer Treat. Rev. 1980, 7, 167-176. Jhalani, V. K.; Ghalsasi, L. P.; Acharya, S. P. and Usgaonkar, R. N. 35 Indian J. Chem., 1989, 28B, 173.

WO 03/097642 PCT/AU03/00569 - 70 Judson, I.R. Semin. Oncol., 1992, 687. Khattab, A.F. 5 Liebigs Ann., 1996, 393. Koller, C.A.; Kantarjian, H.M.; Feldman, E.J.; O'Brien, S.; Rios, M.B.; Estey, E. and Keating, M. Cancer, 1999, 86, 2246. 10 Kubo, K.; Ukawa, K.; Kuzuna, S. and Nohara, A. Chem. Pharm. Bull. 1986; 34; 1108. Leaf, A. N.; Neuberg, D.; Schwartz, E. L.; Wadler, S.; 15 Ritch, P. S.; Dutcher, J. P.; Adams, G. L. Inv. New Drugs, 1997, 15, 165. Marshall, E. S.; Finlay, G. J.; Matthews, J. H. L.; Shaw, J. H. F.; Nixon, J. and Baguley, B. C. 20 J. Natl. Cancer Inst., 1992, 84, 340. Marshall, E.S.; Matthews, J.H.L.; Shaw, J.H.F.; Nixon, J.; Tumewu, P.; Finlay, G.J.; Holdaway, K.M.; Baguley, B.C. Eur. J. Cancer 1994, 30A, 1370. 25 McFadyen, W.D.; Grant, L-C and Denny, W.A. Proceedings of the 13th Annual Conference of the Royal Australian Chemical Institute Medicinal and Agricultural Division 1996, Abstract 5-5. 30 Meth-Cohn, O. Sth. Afr. J. Chem., 1987, 40, 189. Modi, A. R. and Usgaonkar, R. N. 35 Indian J. Chem., 1979, 18B, 304.

WO 03/097642 PCT/AU03/00569 - 71 Riou, J-F.; Ross6, P.; Nguyen, C.H.; Larsen, A.K.; Bissery, M-C.; Grondard, L.; Saucier, J-M.; Bisagni, E. and Lavelle, F. Cancer Res., 1993, 53, 5987. 5 Rivalle, C. and Bisagni, E. J. Heterocycl. Chem., 1980, 17, 245. Sami, S. M.; Dorr, R. T.; Alberts, D. S.; Solyom, A. M. 10 and Remers, W. A. J. Med. Chem., 1996, 39, 4978. Skehan, P.; Storeng. R.; Scudiero, D.; Monks, A.; McMahon. J.; Vistica, D.; Warren, J.T.; Bokesch, H.; Kenney. S. and 15 Boyd, M.R. J. Natl. Cancer Inst. 1990, 82, 1107. Stefanska, B.; Dzieduszycka, M.; Martelli, S.; Tarasiuk, J.; Bontemps-Gracz, M. and Borowski, E. 20 J. Med. Chem., 1993, 36, 38. Stewart, A.J.; Mistry, P.; Dangerfield, W.; Bootle, D.; Baker, M.; Kofler, B.; Okiji, S,; Baguley, B.C.; Denny, W.A. and Charlton, P.A. 25 Anticancer Drugs, 2001, 12, 359. Thompson, J.; Pratt, C.B.; Stewart, C.F.; Avery, L.; Bowman, L.; Zamboni, W.C. and Pappo, A. Inv. New Drugs, 1998, 16, 45. 30 Utsugi, T.; Aoyagi, K.; Furune, Y.; Sano, M.; Wierzba, K.; Okazaki, S.; Asao, T. and Yamada, Y. Proc. Am. Assoc. Cancer Res., 1996, 37, 427 (abstr. 2915). 35 Vicker, N.; Burgess, L.; Chuckowree, I. S.; Dodd, R.; Folkes, A. J.; Hardick, D. J.; Nancox, D. C.; Miller, W.; Milton, J.; Sohal, S.; Wang, S. M.; Wren, S. P.; Charlton, WO 03/097642 PCT/AU03/00569 - 72 P. A.; Dangerfield, W.; Liddle, C.; Mistry, P.; Stewart, A. J. and Denny, W. A. J. Med. Chem., 2002, 45, 721. 5 Vijayalakshmi, S. and Rajendran, S.P. Indian J. Chem., 1994, 33B, 159. It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be 10 made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.

Claims

1. A compound of the formula I a 9 10 II1 5 Z 7 N 32 6 5 4 W I 10 in which positional numbering, where mentioned, refers to the system illustrated above, and one or more W and one or more Z are attached to a ring carbon or carbons at any of positions 3,4 and 6 to 10, and in which: 15 Q is O or S; W is C(=Q)NR-M-(CH 2 )mRl, in which M is CHJ or G, R is H or an optionally substituted C 1 - 4 alkyl group, 20 J is H or an optionally substituted Cl-C 6 alkyl group, G is an optionally substituted fully saturated, or partially unsaturated, or aromatic, carbocycle or heterocycle, 25 R is C(=NR 2) NH 2 , NHC(=NR 3 )NH 2 or NR 4 R 5 , in which each of R 2 and R 3 are independently H or an optionally substituted C 1 -4 alkyl group, R 4 and R s 5 are independently H or an optionally substituted C 1 - 4 alkyl group or R 4 and R 5 together with the 30 nitrogen atom to which they are attached form an optionally substituted, saturated or unsaturated heterocyclic group, and m is an integer from 0 to 6; Y is H, C 1 -6 alkyl or (CH 2 )n-X-(CH 2 )pU, in which 35 X is CH 2 , C=O, CH=CH, O, S, NR or G; and n and p are integers from 0 to 6, and U is H, CF 3 , halo, NR 4 R 5 , +NRR 4 R 5 , cyano, WO 03/097642 PCT/AU03/00569 - 74 C(=O)NRR 5 , OR 4 , CO 2 R 4 , G, NR 4 G or OG; and Z is H, halo, OH, CO 2 H, C0 2 R 4 , SO 2 R 4 , NR 4 R 5 , nitro, cyano, C1-6 alkyl, CI-6 haloalkyl, CI-6 alkoxy, C16 haloalkoxy, C 1 -6 aminoalkyl, CI-6 aminoalkoxy, or aza 5 functionality replacing a ring CH functionality, or a carbon or carbon/nitrogen framework bridging the 6-7, 7-8 or 8-9 positions so as to form an additional fused 5 to 6 membered carbocycle or heterocycle; or a pharmaceutically-acceptable salt, N-oxide, 10 hydrate, solvate, pharmaceutically acceptable derivative, pro-drug, tautomer and/or isomer thereof.

2. A compound according to claim 1, in which the optional substituents for R, R 2 , R , R and R 5 are one or 15 more OH or NH 2 groups.

3. A compound according to claim 1 or claim 2, in which the optional substituents for J are one or more OH, OMe, NH 2 , NHMe or NMe 2 groups. 20

4. A compound according to any one of claims 1 to 3, in which the substituents for G are one or more halo, OH, CO 2 H, NR 4 R 5 , NRSO 2 R, SO 2 NR 4 R 5 , nitro, cyano, C1-6 alkyl, C1-6 haloalkyl, C1-6 alkoxy, Ci-6 haloalkoxy, CI-6 aminoalkyl, 25 C-6 aminoalkoxy or B(OR 6 ) (OR 7 ) in which R 6 and R 7 are independently hydrogen or an optionally substituted C1-4 alkyl group or together with the 0 and B atoms to which they are attached form an optionally substituted, fully saturated, or partially unsaturated, heterocycle. 30

5. A compound according to any one of claims 1 to 4, in which Q is O; W is CONH(CH 2 ) 2 N(CH 3 ) 2 or CONHCH(CH 3 )CH 2 N(CH 3 ) 2 ; Y is methyl, butyl or methoxy substituted phenyl; and Z is H, Cl, methyl or methoxy. 35

6. A compound according to any one of claims 1 to 5, which is N-[2-(dimethylamino)ethyl]-2,6-dimethyl-1-oxo- WO 03/097642 PCT/AU03/00569 - 75 1,2-dihydrobenzo[b][1,6]naphthyridine-4-carboxamide or a pharmaceutically acceptable salt or N-oxide thereof.

7. A compound of the formula II or III 5 Q 9 10 8 1 zN- -L z 7 3 10 6 5 4 W x II Q 9 10 81 Y 15 8 el'Y - 3 7 N 6 5 4 CN(R) -L 5 x - Q III 20 in which one or more W and one or more Z in formula II, and one or more Z and C(=Q)NR in formula III are attached to a ring carbon or carbons at any of positions 3, 4 and 6 to 10 and in which: Q, R, W, Y and Z are as defined in formula I 25 according to any one of claims 1 to 6; x is an integer from 2 to 4; and L is a linker group of valency x; or a pharmaceutically-acceptable salt, N-oxide, hydrate, solvate, pharmaceutically acceptable derivative, pro-drug, 30 tautomer and/or isomer thereof.

8. A compound according to claim 7, in which x is 2. 35

9. A compound according to claim 7 or claim 8, in which L is O; S; an optionally substituted C 1 - 20 alkylene, alkenylene or alkynylene chain which may optionally be WO 03/097642 PCT/AU03/00569 - 76 interspersed with one or more optionally substituted aryl or optionally substituted heterocyclic groups and/or one or more O, S or N atoms; or an optionally substituted saturated or unsaturated aryl or heterocyclic group. 5

10. A compound according to any one of claims 7 to 9, in which L is a nitrogen-containing linker group.

11. A compound according to any one of claims 7 to 10 10 in which L is V- [ (CHR)qNR 4 ]r(CHR)q-V 2 in which V 1 and V 2 are each independently oxygen, NR or CHR; q is an integer from 0 to 5 optionally having a different value for each subunit of the linker L; 15 r is an integer from 0 to 2; R 4 is as defined in formula I according to any one of claims 1 to 6, or when r is greater than 0, R and R 4 may together form an optionally substituted branched or straight chained alkylene. 20

12. A compound according to claim 11, in which R and R 4 are each independently H, CH 3 , or C 2 H 5 , or if r is greater than 0, R and R 4 are -CH 2 CH 2 CH 2 -. 25

13. A compound according to any one of claims 7 to 12, in which L is selected from the following: -(CH 2 ) 2 NH(CH 2 ) 2 -(CH 2 ) 3 -NMe-(CH 2 )3 -(CH 2 ) 2 NH(CH 2 ) 2 NH(CH 2 ) 2 30 -(CH 2 ) 2 NH(CH 2 ) 3 NH(CH 2 ) 2 -(CH 2 ) 2 NMe(CH 2 ) 2 NMe(CH 2 )2 -(CH 2 ) 2 NMe(CH 2 ) 3 NMe(CH 2 )2 -N,N'-Bis(ethylene)piperazine -N,N'-Bis(propylene)piperazine-, 35 -(CH 2 )sNH(CH 2 )t -(CH 2 )sNAlkyl(CH 2 )t -(CH 2 )sNH(CH 2 )tNH(CH2)u-, and WO 03/097642 PCT/AU03/00569 - 77 -(CH 2 )sNAlkyl(CH 2 )tNAlkyl(CH 2 )u - , in which s, t and u are integers from 2 to 6.

14. A process for the preparation of a compound of 5 formula I according to any one of claims 1 to 6 or a compound of formula II or III according to any one of claims 7 to 13 which comprises converting a compound of formula IV 9 10 8 1 Y 10z 2 7 N '3 6 5 4 COH IV 15 in which one or more Z and one or more CO 2 H groups are attached to a ring carbon or carbons at any of positions 3, 4 and 6 to 10; and Q, Y and Z are as defined in formula I into the 20 amide of formula I, II or III.

15. A process according to claim 14, in which the conversion step involves an intermediate step in which the compound of formula IV is converted into an imidazolide 25 and then reacted with the appropriate amine to obtain the amide of formula I, II or III.

16. A process according to claim 14, in which the carboxylic acid of formula IV is converted into an acid 30 halide, followed by reaction with an amine to obtain the amide of formula I, II or III.

17. A pharmaceutical or veterinary composition comprising the compound of formula I according to any one 35 of claims 1 to 6 or the compound of formula II or III according to any one of claims 7 to 13, together with a pharmaceutically or veterinarily acceptable carrier. WO 03/097642 PCT/AU03/00569 - 78

18. A method of treatment and/or prophylaxis of a cellular proliferative disorder comprising administering a therapeutically effective amount of the compound of 5 formula I according to any one of claims 1 to 6 or the compound of formula II or III according to any one of claims 7 to 13 to a subject in need thereof.

19. A method according to claim 18, in which 10 cellular proliferative disorder is a neoplasm, tumour or a cancer.

20. A method according to claim 18 or claim 19, in which the cellular proliferative disorder is a cancer of 15 the breast, lung, prostate, kidney, skin, neural, ovary, uterus, liver, pancreas, epithelial, gastric, intestinal, exocrine, endocrine, lymphatic, haematopoietic system and/or head and neck tissue. 20

21. A method according to any one of claims 18 to 20, in which the cellular proliferative disorder is leukaemia, lymphoma, multiple myeloma, sarcoma, a brain tumour, or a cancer of the lung, breast, ovary, testes and/or colon. 25

22. A method according to any one of claims 18 to 21, in which the compound of formula I, II or III is administered separately, sequentially or simultaneously with another medicament. 30

23. A method according to claim 22, in which the medicament is an anti-neoplastic agent, a second DNA binding anti-cancer therapeutic agent and/or a side effect relieving agent. 35

24. A method according to any one of claims 18 to 23, in which the compound of formula I, II or III is WO 03/097642 PCT/AU03/00569 - 79 administered in the form of a tumour-activated prodrug in which the compound is linked to a trigger domain.

25. Use of the compound of formula I according to any 5 one of claims 1 to 6 or the compound of formula II or III according to any one of claims 7 to 13 in the manufacture of a medicament for the treatment and/or prophylaxis of a cellular proliferative disorder. 10

26. The compound of formula I according to any one of claims 1 to 6 or the compound of formula II or II according to any one of claims 7 to 13 for use in the treatment and/or prophylaxis of a cellular proliferative disorder. 15

27. Use of the compound of formula I according to any one of claims 1 to 6 or the compound of the formula II or II according to any one of claims 7 to 13 as a cytotoxic, anti-neoplastic, anti-tumour and/or anti-cancer agent. 20

28. A compound of formula IV according to claim 14 with the proviso that when Z is CH 3 and attached to a ring carbon at position 6, CO 2 H is attached to a ring carbon at position 4 and Q is O, then Y is not CH 3 . 25

29. A process for the preparation of a compound of formula IV according to claim 28, in which Q is 0 and CO 2 H is attached to position 4 comprising reacting a compound O of formula V, 9 10 01 30 8 0s O Z 3 7 N4 "O 6 5 CHNMe 2 V 35 in which Z is as defined in formula I according to any one of claims 1 to 6, with YNH 2 in which Y is as defined in formula I. SUBSTITUTE SHEET(RULE26)