AU2003292338A8 - Molecular identification of bacteria of genus less thanigreater thanstreptococcusless than/igreater than and related genuses - Google Patents
Molecular identification of bacteria of genus less thanigreater thanstreptococcusless than/igreater than and related genusesInfo
- Publication number
- AU2003292338A8 AU2003292338A8 AU2003292338A AU2003292338A AU2003292338A8 AU 2003292338 A8 AU2003292338 A8 AU 2003292338A8 AU 2003292338 A AU2003292338 A AU 2003292338A AU 2003292338 A AU2003292338 A AU 2003292338A AU 2003292338 A8 AU2003292338 A8 AU 2003292338A8
- Authority
- AU
- Australia
- Prior art keywords
- bacteria
- thanstreptococcusless
- thanigreater
- igreater
- genuses
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 8
- 239000000203 mixture Substances 0.000 abstract 4
- 239000012634 fragment Substances 0.000 abstract 3
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 abstract 2
- 229930010555 Inosine Natural products 0.000 abstract 2
- 229960003786 inosine Drugs 0.000 abstract 2
- 241000201860 Abiotrophia Species 0.000 abstract 1
- 241000194033 Enterococcus Species 0.000 abstract 1
- 241000193789 Gemella Species 0.000 abstract 1
- 241000235796 Granulicatella Species 0.000 abstract 1
- 241000194017 Streptococcus Species 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 101150090202 rpoB gene Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- External Artificial Organs (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The rpoB gene (I), and its fragments, of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia and Granulicatella, comprising any of 28 sequences (II), reproduced (some degenerate), are new. Independent claims are also included for the following: (1) oligonucleotides (ON) containing at least 12 consecutive nucleotides (nt) from sequences (S6) or (S7), or the reverse sequences or their complements; (2) equimolar mixture of 16 or 4 ON: (a) containing at least 18, preferably 20, consecutive nt present in (S6); or (b) at least 21, preferably 23, consecutive nt present in (S7), where N = A,C,G,T in the mixture of 16 ON or inosine in the mixture of 4 ON; (3) detecting bacteria of the specified genera by molecular identification that uses (I) or its fragments, or at least one ON; and (4) kit for method (3) containing at least one ON, a mixture of ON or a fragment of (I). 5'-AARYTNGGMCCTGAAGAAAT (S6) 5'-TGNARTTTRTCATCAACCATGTG (S7) N = inosine or any of A, T, C or G; R = A or G; Y = C or T; M = A or C.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR02/13792 | 2002-11-05 | ||
| FR0213792A FR2846668B1 (en) | 2002-11-05 | 2002-11-05 | MOLECULAR IDENTIFICATION OF BACTERIA OF THE GENUS STREPTOCOCCUS AND RELATED GENRES |
| PCT/FR2003/003293 WO2004041841A2 (en) | 2002-11-05 | 2003-11-04 | Molecular identification of bacteria of genus streptococcus and related genuses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2003292338A8 true AU2003292338A8 (en) | 2004-06-07 |
| AU2003292338A1 AU2003292338A1 (en) | 2004-06-07 |
Family
ID=32104434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2003292338A Abandoned AU2003292338A1 (en) | 2002-11-05 | 2003-11-04 | Molecular identification of bacteria of genus less thanigreater thanstreptococcusless than/igreater than and related genuses |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7601822B2 (en) |
| EP (1) | EP1558637B1 (en) |
| JP (1) | JP4717441B2 (en) |
| AT (1) | ATE447582T1 (en) |
| AU (1) | AU2003292338A1 (en) |
| DE (1) | DE60329929D1 (en) |
| ES (1) | ES2335987T3 (en) |
| FR (1) | FR2846668B1 (en) |
| WO (1) | WO2004041841A2 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI115637B (en) * | 2003-12-19 | 2005-06-15 | Mobidiag Oy | Diagnostic Method for Detection and Identification of Bacteria Causing Respiratory Infections and Primer Mixture for Use in the Method |
| JP5137443B2 (en) * | 2006-03-31 | 2013-02-06 | キヤノン株式会社 | Probe set, probe fixing carrier, and genetic testing method |
| JP2010524454A (en) | 2007-04-19 | 2010-07-22 | モレキュラー ディテクション インコーポレーテッド | Methods, compositions and kits for detection and analysis of antibiotic resistant bacteria |
| WO2008153893A2 (en) * | 2007-06-05 | 2008-12-18 | New York University School Of Medicine | Methods and species-specific primers for detection and quantification of streptococcus mutans and streptococcus sanguinis in mixed bacterial samples |
| WO2011040820A1 (en) * | 2009-09-30 | 2011-04-07 | Fjell & Fjord Mat As | Lactic acid bacteria suitable for producing partly fermented meat and a process for producing partly fermented meat by establishing conditions suitable for the proliferation of such lactic acid bacteria |
| US8877909B2 (en) | 2011-04-04 | 2014-11-04 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus |
| EP2862942A1 (en) * | 2013-10-17 | 2015-04-22 | Genomica S.A.U. | Detection of Streptococcus pneumoniae |
| CN110317890A (en) * | 2019-06-24 | 2019-10-11 | 浙江大学 | A kind of kit for enterococcus faecalis detection |
| CN110358848A (en) * | 2019-06-24 | 2019-10-22 | 浙江大学 | A kind of kit for enterococcus faecium detection |
| CN120174125B (en) * | 2025-05-20 | 2025-08-08 | 南京农业大学 | Dual-fluorescence quantitative PCR kit for simultaneously detecting streptococcus pastoris and streptococcus suis |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998018931A2 (en) * | 1996-10-31 | 1998-05-07 | Human Genome Sciences, Inc. | Streptococcus pneumoniae polynucleotides and sequences |
| US6583275B1 (en) * | 1997-07-02 | 2003-06-24 | Genome Therapeutics Corporation | Nucleic acid sequences and expression system relating to Enterococcus faecium for diagnostics and therapeutics |
| US6617156B1 (en) * | 1997-08-15 | 2003-09-09 | Lynn A. Doucette-Stamm | Nucleic acid and amino acid sequences relating to Enterococcus faecalis for diagnostics and therapeutics |
| EP1268774A2 (en) * | 2000-03-21 | 2003-01-02 | Elitra Pharmaceuticals, Inc. | Identification of essential genes in prokaryotes |
| GB0107658D0 (en) * | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
-
2002
- 2002-11-05 FR FR0213792A patent/FR2846668B1/en not_active Expired - Fee Related
-
2003
- 2003-11-04 JP JP2004549286A patent/JP4717441B2/en not_active Expired - Fee Related
- 2003-11-04 AU AU2003292338A patent/AU2003292338A1/en not_active Abandoned
- 2003-11-04 WO PCT/FR2003/003293 patent/WO2004041841A2/en not_active Ceased
- 2003-11-04 EP EP03767905A patent/EP1558637B1/en not_active Expired - Lifetime
- 2003-11-04 AT AT03767905T patent/ATE447582T1/en not_active IP Right Cessation
- 2003-11-04 US US10/529,319 patent/US7601822B2/en not_active Expired - Fee Related
- 2003-11-04 DE DE60329929T patent/DE60329929D1/en not_active Expired - Lifetime
- 2003-11-04 ES ES03767905T patent/ES2335987T3/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004041841A2 (en) | 2004-05-21 |
| FR2846668B1 (en) | 2007-12-21 |
| ATE447582T1 (en) | 2009-11-15 |
| ES2335987T3 (en) | 2010-04-07 |
| US7601822B2 (en) | 2009-10-13 |
| JP2006505262A (en) | 2006-02-16 |
| JP4717441B2 (en) | 2011-07-06 |
| WO2004041841A3 (en) | 2004-09-02 |
| FR2846668A1 (en) | 2004-05-07 |
| EP1558637A2 (en) | 2005-08-03 |
| US20060199182A1 (en) | 2006-09-07 |
| DE60329929D1 (en) | 2009-12-17 |
| AU2003292338A1 (en) | 2004-06-07 |
| EP1558637B1 (en) | 2009-11-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase | ||
| TH | Corrigenda |
Free format text: IN VOL 18, NO 25, PAGE(S) 7031 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME UNIVERSITE DE LA MEDITERRANEE (AIX-MARSEILLE II), APPLICATION NO. 2003292338, UNDER INID (71) CORRECT THE NAME TO READ UNIVERSITE DE LA MEDITERRANEE (AIX-MARSEILLE II); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) |