[go: up one dir, main page]

AU2003273995A8 - Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites - Google Patents

Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Info

Publication number
AU2003273995A8
AU2003273995A8 AU2003273995A AU2003273995A AU2003273995A8 AU 2003273995 A8 AU2003273995 A8 AU 2003273995A8 AU 2003273995 A AU2003273995 A AU 2003273995A AU 2003273995 A AU2003273995 A AU 2003273995A AU 2003273995 A8 AU2003273995 A8 AU 2003273995A8
Authority
AU
Australia
Prior art keywords
synthesis
compositions
methods
nucleic acid
acid molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003273995A
Other versions
AU2003273995A1 (en
Inventor
James L Hartley
Michael A Brasch
John Carrino
James Fan
David Cheo
Devon R N Byrd
Knut Madden
Jonathan D Chesnut
Louis Leong
Martin Gleeson
Gary F Temple
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2003273995A8 publication Critical patent/AU2003273995A8/en
Publication of AU2003273995A1 publication Critical patent/AU2003273995A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003273995A 2002-06-05 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites Abandoned AU2003273995A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38561302P 2002-06-05 2002-06-05
US60/385,613 2002-06-05
PCT/US2003/018036 WO2003103600A2 (en) 2002-06-05 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Publications (2)

Publication Number Publication Date
AU2003273995A8 true AU2003273995A8 (en) 2003-12-22
AU2003273995A1 AU2003273995A1 (en) 2003-12-22

Family

ID=29736108

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003273995A Abandoned AU2003273995A1 (en) 2002-06-05 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Country Status (3)

Country Link
EP (1) EP1539998A4 (en)
AU (1) AU2003273995A1 (en)
WO (1) WO2003103600A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CA2307016A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE60042969D1 (en) 1999-03-02 2009-10-29 Life Technologies Corp PREPARATIONS AND METHODS FOR USE IN RECOMBINATORY CLONING OF NUCLEIC ACIDS
NZ553687A (en) 2000-03-30 2010-03-26 Whitehead Biomedical Inst RNA sequence-specific mediators of RNA interference
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
TR200401292T3 (en) 2000-12-01 2004-07-21 Max@Planck@Gesellschaft�Zur�F�Rderung�Der�Wissenschaften the rnaágirişimineáyoláaçanáküçükárnaámolekül
US8729036B2 (en) 2002-08-07 2014-05-20 University Of Massachusetts Compositions for RNA interference and methods of use thereof
ATE469984T1 (en) 2003-12-01 2010-06-15 Life Technologies Corp NUCLEIC ACID MOLECULES CONTAINING RECOMBINATION SITE AND METHOD FOR USE THEREOF
CN1981039A (en) * 2004-05-10 2007-06-13 巴斯福植物科学有限公司 Methods for assembling multiple expression constructs
US7622252B2 (en) 2005-06-10 2009-11-24 Baylor College Of Medicine Generation of minicircle DNA with physiological supercoiling
BR112012011576A2 (en) 2009-10-16 2015-09-15 Baylor College Medicine Supercoiled Mini Circles DNA for Gene Therapy Applications
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites
AU8835601A (en) * 2000-08-21 2002-03-04 Invitrogen Corp Methods and reagents for molecular cloning
AU2003209054A1 (en) * 2002-02-07 2003-09-02 Discovery Genomics, Inc. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation and methods of use thereof

Also Published As

Publication number Publication date
WO2003103600A3 (en) 2004-10-07
EP1539998A4 (en) 2007-10-31
AU2003273995A1 (en) 2003-12-22
WO2003103600A2 (en) 2003-12-18
EP1539998A2 (en) 2005-06-15

Similar Documents

Publication Publication Date Title
AU2002318386A1 (en) Methods for characterization of nucleic acid molecules
AU2003287299A1 (en) Process for concentration of macromolecules
AU2003270310A8 (en) Compositions and methods for synthesizing nucleic acids
EP1451344A4 (en) Compositions and methods for use in isolation of nucleic acid molecules
AU2002365811A1 (en) Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations
AU2003230957A8 (en) Quantitation of biological molecules
AU2003273995A8 (en) Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1390394A4 (en) Compositions and methods for recombinational cloning of nucleic acid molecules
AU2003280603A8 (en) Method of amplifying nucleic acid
GB0500265D0 (en) Methods and compositions relating to labeled rna molecules that reduce gene expression
AU2003260291A8 (en) Proximity-aided synthesis of templated molecules
AU2003303588A1 (en) Methods and compositions for sequencing nucleic acid molecules
AU2003250506A8 (en) Method for the characteristation of nucleic acid molecules
IL173107A0 (en) Compositions and methods for purifying and crystallizing molecules of interest
EG24152A (en) Novel method for the synthesis of s-indoline-2-carboxylic acid and application thereof in synthesis of perindopril
AU2003211867A1 (en) Novel method of highly sensitive nucleic acid analysis
AU2002306850A1 (en) Methods and reagents for decreasing clinical reaction to allergy
AU2003268478A1 (en) Cardiolipin molecules and method of synthesis
AU2003232036A8 (en) Nanoarchaeum nucleic acid molecule
PT3222724T (en) Further novel forms of interfering rna molecules
GB0320415D0 (en) Analysis of macromolecules
HK1079551A (en) Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
GB0212853D0 (en) Separation of molecules
EP1614750A4 (en) Novel functional peptide nucleic acid and process for producing the same
GB2392746B (en) Comparison of molecules using field points

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase