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AU2002221829A1 - Modified recombinase - Google Patents

Modified recombinase

Info

Publication number
AU2002221829A1
AU2002221829A1 AU2002221829A AU2182902A AU2002221829A1 AU 2002221829 A1 AU2002221829 A1 AU 2002221829A1 AU 2002221829 A AU2002221829 A AU 2002221829A AU 2182902 A AU2182902 A AU 2182902A AU 2002221829 A1 AU2002221829 A1 AU 2002221829A1
Authority
AU
Australia
Prior art keywords
recombinase
fusion protein
genome
cell
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002221829A
Inventor
Nicole Faust
Susanne Felder
Ralf Kuhn
Birgit Kuter-Luks
Frieder Schwenk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Artemis Pharmaceuticals GmbH
Original Assignee
Artemis Pharmaceuticals GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00124629A external-priority patent/EP1205490A1/en
Application filed by Artemis Pharmaceuticals GmbH filed Critical Artemis Pharmaceuticals GmbH
Publication of AU2002221829A1 publication Critical patent/AU2002221829A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention concerns a fusion protein comprising a recombinase protein, preferably the site-specific DNA recombinase C31-Int of phage (C31, and a peptide sequence which directs the nuclear uptake of the fusion protein in eucaryotic cells, and the use of this fusion protein to recombine, invert or delete DNA molecules containing recognition sequences for said recombinase in eucaryotic cells at high efficiency. In addition the invention relates to a cell, preferably a mammalian cell which contains recognition sequences for said recombinase in its genome and wherein the genome is recombined by the action of said fusion protein. Moreover, the invention relates to the use of said cell to study the function of genes and for the creation of transgenic organisms to study gene function at various developmental stages, including the adult. In conclusion, the present invention provides a process which enables the highly efficient modification of the genome of mammalian cells by site-specific recombination.
AU2002221829A 2000-11-10 2001-11-09 Modified recombinase Abandoned AU2002221829A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP00124629A EP1205490A1 (en) 2000-11-10 2000-11-10 Fusion protein comprising integrase (phiC31) and a signal peptide (NLS)
EP00124629.7 2000-11-10
EP01109543.7 2001-04-17
EP01109543 2001-04-17
US31187601P 2001-08-13 2001-08-13
US60/311,876 2001-08-13
PCT/EP2001/012975 WO2002038613A2 (en) 2000-11-10 2001-11-09 Modified recombinase

Publications (1)

Publication Number Publication Date
AU2002221829A1 true AU2002221829A1 (en) 2002-05-21

Family

ID=56290218

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002221829A Abandoned AU2002221829A1 (en) 2000-11-10 2001-11-09 Modified recombinase

Country Status (5)

Country Link
EP (1) EP1395612B1 (en)
AT (1) ATE303403T1 (en)
AU (1) AU2002221829A1 (en)
DE (1) DE60113159T2 (en)
WO (1) WO2002038613A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001239792B2 (en) * 2000-02-18 2006-05-18 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
WO2003066867A2 (en) * 2002-02-06 2003-08-14 Artemis Pharmaceuticals Gmbh Genetically engineered phic31-integrase genes
DE10207313A1 (en) * 2002-02-21 2003-09-11 Vision 7 Gmbh In vitro or in vivo site-specific DNA recombination, useful e.g. for gene inactivation, using Cre recombinase that lacks heterologous protein-transduction domain
EP1439234A1 (en) * 2003-01-08 2004-07-21 ARTEMIS Pharmaceuticals GmbH Targeted transgenesis using the rosa26 locus
EP1483962A3 (en) * 2003-06-06 2005-01-12 F. Hoffmann-La Roche Ag Transgenic animal model for Glyt1 function
US9034650B2 (en) * 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
WO2007137267A2 (en) * 2006-05-22 2007-11-29 Hiprocell, Llc Protein production using eukaryotic cell lines
CN100410369C (en) * 2006-06-22 2008-08-13 复旦大学 ΦC31 integrase protein modified by TAT and NLS polypeptide and its application
WO2008079943A2 (en) * 2006-12-21 2008-07-03 Smithkline Beecham Corporation Novel methods

Also Published As

Publication number Publication date
WO2002038613A3 (en) 2004-01-08
DE60113159T2 (en) 2006-07-06
EP1395612A2 (en) 2004-03-10
DE60113159D1 (en) 2005-10-06
ATE303403T1 (en) 2005-09-15
EP1395612B1 (en) 2005-08-31
WO2002038613A2 (en) 2002-05-16

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