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AU2002221540A1 - Method for the simultaneous determination of two fluorescent emissions with a single laser flow cytometer - Google Patents

Method for the simultaneous determination of two fluorescent emissions with a single laser flow cytometer

Info

Publication number
AU2002221540A1
AU2002221540A1 AU2002221540A AU2154002A AU2002221540A1 AU 2002221540 A1 AU2002221540 A1 AU 2002221540A1 AU 2002221540 A AU2002221540 A AU 2002221540A AU 2154002 A AU2154002 A AU 2154002A AU 2002221540 A1 AU2002221540 A1 AU 2002221540A1
Authority
AU
Australia
Prior art keywords
flow cytometer
single laser
simultaneous determination
fluorescent emissions
laser flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002221540A
Inventor
Michael Debatin
Hubert Hug
Karsten Stahnke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Publication of AU2002221540A1 publication Critical patent/AU2002221540A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AU2002221540A 2000-10-30 2001-10-30 Method for the simultaneous determination of two fluorescent emissions with a single laser flow cytometer Abandoned AU2002221540A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10053747.2 2000-10-30
DE2000153747 DE10053747C2 (en) 2000-10-30 2000-10-30 Method for the simultaneous determination of two fluorescence emissions with a single laser flow cytometer
PCT/DE2001/004125 WO2002040978A2 (en) 2000-10-30 2001-10-30 Method for the simultaneous determination of two fluorescent emissions with a single laser flow cytometer

Publications (1)

Publication Number Publication Date
AU2002221540A1 true AU2002221540A1 (en) 2002-05-27

Family

ID=7661547

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002221540A Abandoned AU2002221540A1 (en) 2000-10-30 2001-10-30 Method for the simultaneous determination of two fluorescent emissions with a single laser flow cytometer

Country Status (3)

Country Link
AU (1) AU2002221540A1 (en)
DE (1) DE10053747C2 (en)
WO (1) WO2002040978A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003042703A1 (en) * 2001-11-13 2003-05-22 Klaus-Michael Debatin Method of detecting apoptosis induction by measuring the release of substances from cell organelles by means of flow cytometry
US7468518B2 (en) 2003-10-23 2008-12-23 National University Of Singapore Fluorescence correlation spectroscopy with single excitation wavelength
US20110001963A1 (en) * 2009-07-02 2011-01-06 Durack Gary P System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system
CN102472701A (en) 2009-07-06 2012-05-23 索尼公司 Microfluidic device
CN102482631A (en) 2009-07-07 2012-05-30 索尼公司 Microfluidic device suitable for selective extraction of samples following centrifugation and methods of use thereof
CN103383336A (en) 2009-07-07 2013-11-06 索尼公司 Microfluidic device
US9291562B2 (en) 2011-11-15 2016-03-22 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Method and apparatus for tracking a particle, particularly a single molecule, in a sample
DE102011055367B4 (en) * 2011-11-15 2017-02-09 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method and device for tracking a movement of a particle, in particular a single molecule, in a sample
CN108732082A (en) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 cell grouping method

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4867908A (en) * 1986-08-29 1989-09-19 Becton, Dickinson And Company Method and materials for calibrating flow cytometers and other analysis instruments
DE4210970C2 (en) * 1992-04-02 1996-10-17 Markus Dipl Chem Sauer Process for the simultaneous optical qualitative and quantitative detection of different molecules of a mixture marked with fluorochromes or fluorogens by means of laser spectroscopy
US5675517A (en) * 1995-04-25 1997-10-07 Systemix Fluorescence spectral overlap compensation for high speed flow cytometry systems
US6100038A (en) * 1996-09-06 2000-08-08 Litron Laboratories Limited Method for the enumeration of micronucleated erythrocyte populations with a single laser flow cytometer
KR20010031056A (en) * 1997-10-10 2001-04-16 시토비아 인크. Novel fluorescent reporter molecules and their applications including assays for caspases
DE19843873A1 (en) * 1998-09-25 2000-03-30 Univ Halle Wittenberg Assay for cell-surface protease activity useful for diagnostics, uses a fluorogenic substrate comprising rhodamine 110 and fluorescence or laser scanning microscopy

Also Published As

Publication number Publication date
WO2002040978A2 (en) 2002-05-23
WO2002040978A3 (en) 2002-11-28
DE10053747C2 (en) 2002-10-24
DE10053747A1 (en) 2002-05-23

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