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AU2002218129A1 - Method for the detection of cytosine methylations - Google Patents

Method for the detection of cytosine methylations

Info

Publication number
AU2002218129A1
AU2002218129A1 AU2002218129A AU1812902A AU2002218129A1 AU 2002218129 A1 AU2002218129 A1 AU 2002218129A1 AU 2002218129 A AU2002218129 A AU 2002218129A AU 1812902 A AU1812902 A AU 1812902A AU 2002218129 A1 AU2002218129 A1 AU 2002218129A1
Authority
AU
Australia
Prior art keywords
cytosine
detection
amplified
genomic dna
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002218129A
Inventor
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of AU2002218129A1 publication Critical patent/AU2002218129A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Turbines (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for the detection of cytosine methylations in genomic DNA is disclosed, whereby genomic DNA samples are firstly reacted with a chemical reagent, whereupon 5-methylcytosine and cytosine react differently and thus display a different base pair relationship in the DNA duplex after said reaction. The pre-treated DNA is then amplified by means of a polymerase and at least one primer oligonucleotide and the amplified material once again reacted with a chemical reagent as above. The cytosine bases and/or guanine bases remaining in the amplified material after the renewed chemical treatment are detected in the final step.
AU2002218129A 2000-10-10 2001-10-10 Method for the detection of cytosine methylations Abandoned AU2002218129A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10050942A DE10050942B4 (en) 2000-10-10 2000-10-10 Method for the detection of cytosine methylations
DE10050942.8 2000-10-10
PCT/DE2001/003901 WO2002031186A2 (en) 2000-10-10 2001-10-10 Method for the detection of cytosine methylations

Publications (1)

Publication Number Publication Date
AU2002218129A1 true AU2002218129A1 (en) 2002-04-22

Family

ID=7659773

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002218129A Abandoned AU2002218129A1 (en) 2000-10-10 2001-10-10 Method for the detection of cytosine methylations

Country Status (7)

Country Link
EP (1) EP1325154B1 (en)
JP (1) JP2004511235A (en)
AT (1) ATE368752T1 (en)
AU (1) AU2002218129A1 (en)
CA (1) CA2425366A1 (en)
DE (2) DE10050942B4 (en)
WO (1) WO2002031186A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10061348C2 (en) * 2000-12-06 2002-10-24 Epigenomics Ag Method for the quantification of cytosine methylations in complex amplified genomic DNA
DE10151055B4 (en) 2001-10-05 2005-05-25 Epigenomics Ag Method for detecting cytosine methylation in CpG islands
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
EP1590362B8 (en) 2003-01-29 2015-06-03 Epigenomics AG Improved method for bisulfite treatment
US7262013B2 (en) 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
CN102517389A (en) * 2003-10-09 2012-06-27 Epi基因组股份公司 Improved bisulfite conversion of DNA
CA2547743C (en) 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
EP3342877B1 (en) * 2004-09-30 2020-01-29 Epigenomics AG Method for providing dna fragments derived from an archived sample
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2803734B1 (en) * 2005-04-01 2017-09-20 Epigenomics AG Improved bisulfite conversion of DNA
CN101331148B (en) 2005-12-14 2012-06-27 霍夫曼-拉罗奇有限公司 New method for bisulfite treatment
US8697357B2 (en) 2008-03-12 2014-04-15 Japan Advanced Institute Of Science And Technology Method for detection of methylcytosine using photoresponsive probe
EP2309005B1 (en) * 2009-08-03 2015-03-04 Epigenomics AG Methods for preservation of genomic DNA sequence complexity

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
DE19754482A1 (en) * 1997-11-27 1999-07-01 Epigenomics Gmbh Process for making complex DNA methylation fingerprints
DE19853398C1 (en) * 1998-11-19 2000-03-16 Epigenomics Gmbh Identification of 5-methylcytosine positions in genomic DNA by chemical modification, amplification and heteroduplex formation
DE19905082C1 (en) * 1999-01-29 2000-05-18 Epigenomics Gmbh Identification of methylation patterns of cytosine in genome DNA comprises chemical treatment to produce different base pairing behavior between cytosine and 5-methylcytosine

Also Published As

Publication number Publication date
CA2425366A1 (en) 2002-04-18
EP1325154A2 (en) 2003-07-09
JP2004511235A (en) 2004-04-15
ATE368752T1 (en) 2007-08-15
DE50112799D1 (en) 2007-09-13
DE10050942B4 (en) 2005-11-17
WO2002031186A2 (en) 2002-04-18
EP1325154B1 (en) 2007-08-01
WO2002031186A3 (en) 2003-03-13
DE10050942A1 (en) 2002-04-18

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application