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AU2001286009A1 - Method for massive directed mutagenesis - Google Patents

Method for massive directed mutagenesis

Info

Publication number
AU2001286009A1
AU2001286009A1 AU2001286009A AU8600901A AU2001286009A1 AU 2001286009 A1 AU2001286009 A1 AU 2001286009A1 AU 2001286009 A AU2001286009 A AU 2001286009A AU 8600901 A AU8600901 A AU 8600901A AU 2001286009 A1 AU2001286009 A1 AU 2001286009A1
Authority
AU
Australia
Prior art keywords
massive
directed mutagenesis
mutagenesis
concerns
directed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001286009A
Inventor
Stephane Blesa
Marc Delcourt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomethodes SA
Original Assignee
Biomethodes SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes SA filed Critical Biomethodes SA
Publication of AU2001286009A1 publication Critical patent/AU2001286009A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gyroscopes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The invention concerns the field of molecular biology and more particularly that of mutagenesis. It concerns a method of high-rate directed mutagenesis, that is the formation of numerous directed mutants in reduced time and with reduced number of steps. Said method is therefore referred to as massive mutagenesis.
AU2001286009A 2000-08-25 2001-08-24 Method for massive directed mutagenesis Abandoned AU2001286009A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0010962A FR2813314B1 (en) 2000-08-25 2000-08-25 MASSIVE DIRECTED MUTAGENESIS PROCESS
FR0010962 2000-08-25
PCT/FR2001/002666 WO2002016606A2 (en) 2000-08-25 2001-08-24 Method for massive directed mutagenesis

Publications (1)

Publication Number Publication Date
AU2001286009A1 true AU2001286009A1 (en) 2002-03-04

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001286009A Abandoned AU2001286009A1 (en) 2000-08-25 2001-08-24 Method for massive directed mutagenesis

Country Status (11)

Country Link
US (1) US7202086B2 (en)
EP (1) EP1311670B1 (en)
JP (1) JP5068917B2 (en)
AT (1) ATE354653T1 (en)
AU (1) AU2001286009A1 (en)
CA (1) CA2420721C (en)
DE (1) DE60126786T2 (en)
DK (1) DK1311670T3 (en)
ES (1) ES2282285T3 (en)
FR (1) FR2813314B1 (en)
WO (1) WO2002016606A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
CA2436214C (en) * 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
FR2823219B1 (en) * 2001-04-10 2003-07-04 Pasteur Institut MUTANTS OF DESOXYCYTIDINE KINASE WITH ENLARGED ENZYMATIC ACTIVITY
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
US7611700B2 (en) 2002-09-09 2009-11-03 Hanall Pharmaceuticals, Co., Ltd. Protease resistant modified interferon alpha polypeptides
ATE428779T1 (en) * 2003-12-18 2009-05-15 Biomethodes METHOD FOR SITE-SPECIFIC MASS MUtagenesis
FR2883885B1 (en) * 2005-04-05 2010-12-10 Biomethodes RECOMBINATION IN VITRO CIRCULAR
FR2886943B1 (en) 2005-06-10 2007-09-07 Biomethodes Sa METHOD OF SELECTING STABLE PROTEINS UNDER STANDARD PHYSICO-CHEMICAL CONDITIONS
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (en) * 2006-02-03 2008-04-18 Biomethodes Sa IMPROVED KANAMYCINE KINASE II AND USES THEREOF
CA2644127A1 (en) * 2006-03-08 2007-09-13 Biomethodes Human interferon-gamma (infgamma) variants
DK3031919T3 (en) * 2007-07-31 2019-01-07 Basf Enzymes Llc Tailored multi-site combination device
WO2014096672A1 (en) 2012-12-17 2014-06-26 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Use of monoclonal antibodies for the treatment of inflammation and bacterial infections
EP3024930A2 (en) 2013-07-26 2016-06-01 Biomethodes Novel variant trichoderma reesei endoglucanases
EP3728620A4 (en) * 2017-12-20 2021-11-03 Basf Se GENE SITUATION SATURATION MUTAGENESIS (GSSM) PROCEDURES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
WO1991015581A1 (en) * 1990-04-05 1991-10-17 Roberto Crea Walk-through mutagenesis
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
DE69934088T2 (en) * 1998-01-09 2007-06-21 University Of Utah Research Foundation, Salt Lake City METHOD FOR THE IN VITRO AMPLIFICATION OF CIRCULAR DNA
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Also Published As

Publication number Publication date
DE60126786D1 (en) 2007-04-05
US7202086B2 (en) 2007-04-10
EP1311670A2 (en) 2003-05-21
ES2282285T3 (en) 2007-10-16
WO2002016606A3 (en) 2002-06-27
WO2002016606A2 (en) 2002-02-28
CA2420721C (en) 2013-11-12
CA2420721A1 (en) 2002-02-28
ATE354653T1 (en) 2007-03-15
US20040048268A1 (en) 2004-03-11
FR2813314B1 (en) 2004-05-07
JP5068917B2 (en) 2012-11-07
DK1311670T3 (en) 2007-06-18
EP1311670B1 (en) 2007-02-21
DE60126786T2 (en) 2007-11-08
JP2004507243A (en) 2004-03-11
FR2813314A1 (en) 2002-03-01

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