AU2001278968A1 - Nested sorting and high throughput screening - Google Patents

Nested sorting and high throughput screening

Info

Publication number: AU2001278968A1
Authority: AU; Australia
Prior art keywords: oligonucleotides; combination; antibodies; epitope; capture agents
Prior art date: 2000-07-19
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU2001278968A

Other languages

English (en)

Inventor

Dana Ault-Riche

Paul D. Kassner

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Pointilliste Inc

Original Assignee

Pointilliste Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-07-19

Filing date

2001-07-18

Publication date

2002-01-30

2001-07-18 Application filed by Pointilliste Inc filed Critical Pointilliste Inc

2002-01-30 Publication of AU2001278968A1 publication Critical patent/AU2001278968A1/en

Status Abandoned legal-status Critical Current

Links

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RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
229910052718 tin Inorganic materials 0.000 description 1
239000011135 tin Substances 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
230000032258 transport Effects 0.000 description 1
230000017105 transposition Effects 0.000 description 1
PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
238000009827 uniform distribution Methods 0.000 description 1
239000012224 working solution Substances 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1
239000011701 zinc Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Urology & Nephrology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Organic Chemistry (AREA)
Hematology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Pathology (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Bioinformatics & Computational Biology (AREA)
General Physics & Mathematics (AREA)
Microbiology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Analysing Materials By The Use Of Radiation (AREA)
Investigating Or Analysing Biological Materials (AREA)

AU2001278968A 2000-07-19 2001-07-18 Nested sorting and high throughput screening Abandoned AU2001278968A1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US21918300P	2000-07-19	2000-07-19
US60/219,183		2000-07-19
PCT/US2001/022821 WO2002006834A2 (fr)	2000-07-19	2001-07-18	Collections de proteines de liaison et de marqueurs, utilisations de ces dernieres pour le tri a plusieurs niveaux et le criblage a grande capacite

Publications (1)

Publication Number	Publication Date
AU2001278968A1 true AU2001278968A1 (en)	2002-01-30

Family

ID=22818211

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU2001278968A Abandoned AU2001278968A1 (en)	2000-07-19	2001-07-18	Nested sorting and high throughput screening

Country Status (6)

Country	Link
US (1)	US20020137053A1 (fr)
EP (1)	EP1301632A2 (fr)
JP (1)	JP2004504607A (fr)
AU (1)	AU2001278968A1 (fr)
CA (1)	CA2415328A1 (fr)
WO (1)	WO2002006834A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN110006873A (zh) *	2019-04-08	2019-07-12	重庆市环卫集团有限公司	基于三维微纳结构增强拉曼光谱的环境污染物检测方法

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP1283909A4 (fr)	2000-05-04	2006-06-07	Univ Yale	Reseaux de proteines a haute densite destines au criblage de l'activite de proteines
DE10054055A1 (de)	2000-10-31	2002-05-23	Nmi Univ Tuebingen	Verfahren zur Analyse von Proteinen
KR20030094404A (ko) *	2001-05-11	2003-12-11	예일 유니버시티	프로테옴 칩을 사용한 단백질 활성의 포괄적인 분석
US7618788B2 (en)	2002-05-10	2009-11-17	Millipore Corporation	Proteome epitope tags and methods of use thereof in protein modification analysis
US7460960B2 (en)	2002-05-10	2008-12-02	Epitome Biosystems, Inc.	Proteome epitope tags and methods of use thereof in protein modification analysis
CA2485560A1 (fr) *	2002-05-10	2004-06-03	Engeneos, Inc.	Sequences de reconnaissance uniques et leurs procedes d'utilisation dans l'analyse de proteines
JP4584828B2 (ja) *	2002-10-15	2010-11-24	アブメトリックス，インコーポレイテッド	短いエピトープに対して向けられる複数組のディジタル抗体、およびその使用
CA2504443A1 (fr) *	2002-10-30	2004-05-13	Pointilliste, Inc.	Procedes de production de groupes marques par polypeptides et systemes de capture contenant ces polypeptides marques
EP1597577A4 (fr) *	2003-02-10	2007-02-21	Pointilliste Inc	Reseaux a assemblage autonome et utilisation de ceux-ci
AU2004225496A1 (en) *	2003-03-26	2004-10-14	President And Fellows Of Harvard College	Determination of proteins and/or other molecules using mass spectroscopy
US20050043510A1 (en) *	2003-04-10	2005-02-24	Moeckli Randolph A.	Affinity purification system using troponin molecules as affinity ligands
TWI323745B (en) *	2003-06-11	2010-04-21	Neupro Technology Co Ltd	Method of microwave-assisted protein array fabrication
EP1548126A1 (fr) *	2003-12-22	2005-06-29	Bio-Rad Pasteur	Support pour acide nucléique de contrôle et usage pour la détection des acides nucléiques.
US7977119B2 (en) *	2004-12-08	2011-07-12	Agilent Technologies, Inc.	Chemical arrays and methods of using the same
US7763423B2 (en) *	2005-09-30	2010-07-27	Pacific Biosciences Of California, Inc.	Substrates having low density reactive groups for monitoring enzyme activity
US8153598B2 (en) *	2005-10-19	2012-04-10	Intrexon Corporation	PKD ligands and polynucleotides encoding PKD ligands
US7855057B2 (en)	2006-03-23	2010-12-21	Millipore Corporation	Protein splice variant/isoform discrimination and quantitative measurements thereof
US8975216B2 (en) *	2006-03-30	2015-03-10	Pacific Biosciences Of California	Articles having localized molecules disposed thereon and methods of producing same
US20080050747A1 (en) *	2006-03-30	2008-02-28	Pacific Biosciences Of California, Inc.	Articles having localized molecules disposed thereon and methods of producing and using same
DE602006001583D1 (de)	2006-04-26	2008-08-07	Borealis Tech Oy	Vernetzbare Polyolefinzusammensetzung enthaltend hochmolekuaren Silanolkondensationskatalysator
US20090252745A1 (en) *	2006-09-15	2009-10-08	Duke University	Amino acids in the HCV core polypeptide domain 3 and correlation with steatosis
CN104237539A (zh) *	2009-04-07	2014-12-24	连接Dx股份有限公司	用于读取救护点测试结果的手持扫描仪系统和方法
US8501406B1 (en)	2009-07-14	2013-08-06	Pacific Biosciences Of California, Inc.	Selectively functionalized arrays
WO2019178033A1 (fr) *	2018-03-12	2019-09-19	Arbor Biotechnologies, Inc.	Découverte de protéines à débit très élevé
JPWO2024071112A1 (fr) *	2022-09-27	2024-04-04

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US110932A (en) *		1871-01-10		Improvement in neck-yokes of harness
US110933A (en) *		1871-01-10		Improvement in steam vacuum-pumps
US137053A (en) *		1873-03-25		Improvement in dies for forming wrench-heads
US115225A (en) *		1871-05-23		Improvement in cotton-bale ties
US10672A (en) *		1854-03-21		Jordan l
US68272A (en) *		1867-08-27		Improvement in ooen-huskee
US58269A (en) *		1866-09-25		Improvement in the manufacture of knives and forks
US3599A (en) *		1844-05-25		Power-loom
US5132242A (en) *	1987-07-15	1992-07-21	Cheung Sau W	Fluorescent microspheres and methods of using them
US5084398A (en) *	1987-11-20	1992-01-28	Creative Biomolecules	Selective removal of immune complexes
US5198346A (en) *	1989-01-06	1993-03-30	Protein Engineering Corp.	Generation and selection of novel DNA-binding proteins and polypeptides
US5639603A (en) *	1991-09-18	1997-06-17	Affymax Technologies N.V.	Synthesizing and screening molecular diversity
US5573905A (en) *	1992-03-30	1996-11-12	The Scripps Research Institute	Encoded combinatorial chemical libraries
US5652128A (en) *	1993-01-05	1997-07-29	Jarvik; Jonathan Wallace	Method for producing tagged genes, transcripts, and proteins
JP3509100B2 (ja) *	1993-01-21	2004-03-22	プレジデントアンドフェローズオブハーバードカレッジ	哺乳動物のストレスプロモーターを利用する化合物の毒性測定方法および診断用キット
WO1996024847A1 (fr) *	1995-02-10	1996-08-15	Smithkline Beecham Corporation	Procede d'identification d'agents actifs sur le plan pharmacologique a l'aide d'une banque d'epitopes marques
GB9624927D0 (en) *	1996-11-29	1997-01-15	Oxford Glycosciences Uk Ltd	Gels and their use
US6165709A (en) *	1997-02-28	2000-12-26	Fred Hutchinson Cancer Research Center	Methods for drug target screening
US6451527B1 (en) *	1997-08-29	2002-09-17	Selective Genetics, Inc.	Methods using genetic package display for selecting internalizing ligands for gene delivery
US20030148263A1 (en) *	1997-08-29	2003-08-07	Selective Genetics, Inc.	Methods and compositions using genetic package display
US6589730B1 (en) *	1997-08-29	2003-07-08	Selective Genetics, Inc.	Methods for identifying protein-protein interactions by selective transduction
WO1999010485A1 (fr) *	1997-08-29	1999-03-04	Selective Genetics, Inc.	Procedes utilisant une presentation de phage pour selectionner des ligands internalisants en vue d'une administration de gene
US6723512B2 (en) *	1997-08-29	2004-04-20	Selective Genetics Inc.	Methods using genetic package display for detecting and identifying protein-protein interactions that facilitate internalization and transgene expression and cells or tissues competent for the same and methods for evolving gene delivery vectors
AU1926899A (en) *	1997-12-19	1999-07-12	Affymetrix, Inc.	Exploiting genomics in the search for new drugs
US6232066B1 (en) *	1997-12-19	2001-05-15	Neogen, Inc.	High throughput assay system
EP1051624A4 (fr) *	1998-01-29	2002-05-02	Glaucus Proteomics B V	Series haute densite pour l'analyse des proteomes et procedes et compositions pour ces derniers
US6780981B1 (en) *	1998-05-15	2004-08-24	Oxford Gene Technology Ip Limited	Libraries of oligomers labeled with different tags
US6682942B1 (en) *	1998-07-14	2004-01-27	Zyomyx, Inc.	Microdevices for screening biomolecules
US6406921B1 (en) *	1998-07-14	2002-06-18	Zyomyx, Incorporated	Protein arrays for high-throughput screening
US20030138973A1 (en) *	1998-07-14	2003-07-24	Peter Wagner	Microdevices for screening biomolecules
US6576478B1 (en) *	1998-07-14	2003-06-10	Zyomyx, Inc.	Microdevices for high-throughput screening of biomolecules
US6197599B1 (en) *	1998-07-30	2001-03-06	Guorong Chin	Method to detect proteins
US6468476B1 (en) *	1998-10-27	2002-10-22	Rosetta Inpharmatics, Inc.	Methods for using-co-regulated genesets to enhance detection and classification of gene expression patterns
US6480791B1 (en) *	1998-10-28	2002-11-12	Michael P. Strathmann	Parallel methods for genomic analysis
US6403309B1 (en) *	1999-03-19	2002-06-11	Valigen (Us), Inc.	Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
US6406840B1 (en) *	1999-12-17	2002-06-18	Biomosaic Systems, Inc.	Cell arrays and the uses thereof
US6686154B2 (en) *	2000-06-05	2004-02-03	Zyomyx, Inc.	Screening of phage displayed peptides without clearing of the cell culture
US20030143612A1 (en) *	2001-07-18	2003-07-31	Pointilliste, Inc.	Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US20040048311A1 (en) *	2002-01-24	2004-03-11	Dana Ault-Riche	Use of collections of binding sites for sample profiling and other applications

2001
- 2001-07-18 JP JP2002512691A patent/JP2004504607A/ja active Pending
- 2001-07-18 WO PCT/US2001/022821 patent/WO2002006834A2/fr not_active Ceased
- 2001-07-18 CA CA002415328A patent/CA2415328A1/fr not_active Abandoned
- 2001-07-18 US US09/910,120 patent/US20020137053A1/en not_active Abandoned
- 2001-07-18 AU AU2001278968A patent/AU2001278968A1/en not_active Abandoned
- 2001-07-18 EP EP01957199A patent/EP1301632A2/fr not_active Withdrawn

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN110006873A (zh) *	2019-04-08	2019-07-12	重庆市环卫集团有限公司	基于三维微纳结构增强拉曼光谱的环境污染物检测方法

Also Published As

Publication number	Publication date
CA2415328A1 (fr)	2002-01-24
WO2002006834A2 (fr)	2002-01-24
WO2002006834A9 (fr)	2002-07-18
US20020137053A1 (en)	2002-09-26
EP1301632A2 (fr)	2003-04-16
JP2004504607A (ja)	2004-02-12
WO2002006834A3 (fr)	2002-10-10

Publication	Publication Date	Title
US20020137053A1 (en)	2002-09-26	Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US20040048311A1 (en)	2004-03-11	Use of collections of binding sites for sample profiling and other applications
US20030143612A1 (en)	2003-07-31	Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
JP4829457B2 (ja)	2011-12-07	抗体模倣物および他の結合タンパク質のタンパク質骨格
US7972993B2 (en)	2011-07-05	Identification of protein binding sites
US7674752B2 (en)	2010-03-09	Functional protein arrays
AU2005241003B2 (en)	2011-05-19	Nucleic-acid programmable protein arrays
US5866341A (en)	1999-02-02	Compositions and methods for screening drug libraries
Breitling et al.	2009	High-density peptide arrays
AU2008345674A1 (en)	2009-07-09	Alternative scaffold protein fusions phage display via fusion to pIX of M13 phage
Walter et al.	2001	High-throughput screening of surface displayed gene products
AU2002211099A1 (en)	2002-06-27	Identification of protein binding sites
JP2013162789A (ja)	2013-08-22	抗体模倣物および他の結合タンパク質のためのタンパク質骨格
AU2001278613A1 (en)	2002-05-23	Functional protein arrays
NZ520698A (en)	2004-03-26	A method of screening for a binding site that interacts with a binding molecule comprising screening a molecular library
WO2001094950A2 (fr)	2001-12-13	Criblage de peptides bacteriophages sans elimination de la culture cellulaire
Konthur et al.	2003	High-throughput applications of phage display in proteomic analyses
Konthur et al.	2002	Automation of phage display for high-throughput antibody development
AU2003212842A1 (en)	2003-09-02	Use of collections of binding sites for sample profiling and other applications
US20030166010A1 (en)	2003-09-04	Custom ligand design for biomolecular filtration and purification for bioseperation
Biro	2008	Design and Production of Specifically and with High Affinity Reacting Peptides
Carnazza et al.	2010	Phage Display technology: new biotechnological applications in synthetic biology

Legal Events

Date	Code	Title	Description
2007-02-22	MK4	Application lapsed section 142(2)(d) - no continuation fee paid for the application

AU2001278968A1 - Nested sorting and high throughput screening - Google Patents

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Landscapes

Applications Claiming Priority (3)

Publications (1)

Family

ID=22818211

Family Applications (1)

Country Status (6)

Cited By (1)

Families Citing this family (25)

Family Cites Families (39)

Cited By (1)

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