AU2001278813A1 - Multiplex pcr method and kit and oligonucleotides for detection and identification of mycobacteria using the multiplex pcr method - Google Patents
Multiplex pcr method and kit and oligonucleotides for detection and identification of mycobacteria using the multiplex pcr methodInfo
- Publication number
- AU2001278813A1 AU2001278813A1 AU2001278813A AU7881301A AU2001278813A1 AU 2001278813 A1 AU2001278813 A1 AU 2001278813A1 AU 2001278813 A AU2001278813 A AU 2001278813A AU 7881301 A AU7881301 A AU 7881301A AU 2001278813 A1 AU2001278813 A1 AU 2001278813A1
- Authority
- AU
- Australia
- Prior art keywords
- pcr method
- multiplex pcr
- mycobacteria
- oligonucleotides
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000007403 mPCR Methods 0.000 title 2
- 238000000034 method Methods 0.000 title 2
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR0054166 | 2000-09-15 | ||
| KR10-2000-0054166A KR100433260B1 (en) | 2000-09-15 | 2000-09-15 | Multiplex pcr, kit and oligonucleotide for detection and identification of mycobacteria using multiplex pcr |
| PCT/KR2001/001385 WO2002022872A1 (en) | 2000-09-15 | 2001-08-14 | Multiplex pcr method and kit and oligonucleotides for detection and identification of mycobacteria using the multiplex pcr method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001278813A1 true AU2001278813A1 (en) | 2002-03-26 |
Family
ID=19688740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001278813A Abandoned AU2001278813A1 (en) | 2000-09-15 | 2001-08-14 | Multiplex pcr method and kit and oligonucleotides for detection and identification of mycobacteria using the multiplex pcr method |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR100433260B1 (en) |
| AU (1) | AU2001278813A1 (en) |
| WO (1) | WO2002022872A1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100446850B1 (en) * | 2001-07-19 | 2004-09-04 | 이혜영 | A method for identifying Micobacteria tuberculosis and non-tuberculosis Micobacteria, together with detecting resistance to an antituberculsis drug of Micobacteria obtained by mutation of rpoB gene |
| KR100454585B1 (en) * | 2001-10-09 | 2004-11-02 | 주식회사 에스제이하이테크 | Microarray comprising probes for Mycobacteria genotyping, M. tuberculosis strain differentiation and antibiotic-resistance detection |
| DE10213537A1 (en) | 2002-03-26 | 2003-10-30 | Hain Lifescience Gmbh | Procedure and differentiation of the Mycobacterium tuberculosis complex |
| DE10215238C1 (en) * | 2002-04-06 | 2003-08-14 | Cytonet Gmbh & Co Kg | Detecting mycobacteria and differentiating between Mycobacterium tuberculosis and Mycobacterium avium, comprises amplifying the 16S rRNA gene |
| JP4304976B2 (en) * | 2002-12-19 | 2009-07-29 | 東ソー株式会社 | Detection method of acid-fast bacteria targeting ribosomal RNA |
| US7638309B2 (en) * | 2003-12-03 | 2009-12-29 | Council Of Science And Industrial Research | Method for detecting pathogenic mycobacteria in clinical specimens |
| US7332597B2 (en) | 2004-06-28 | 2008-02-19 | University Of Kentucky Research Foundation | Primers and probe to identify mycobacterium tuberculosis complex |
| EP2270202A1 (en) * | 2009-07-03 | 2011-01-05 | John Ikonomopoulos | Mycobacterial detection |
| KR101458781B1 (en) * | 2009-08-26 | 2014-11-07 | 주식회사 엘지생명과학 | Composition for detecting mycobacterial and mycobacterial species and simultaneous analysis of mycobacteria and mycobacteria by real-time multiplex polymerase chain reaction |
| US9982255B2 (en) | 2013-03-11 | 2018-05-29 | Kailos Genetics, Inc. | Capture methodologies for circulating cell free DNA |
| CN103555828B (en) * | 2013-10-10 | 2016-04-06 | 上海市肺科医院 | Mycobacterium quick medicine-sensitive detection method and detection target |
| CN109975543B (en) * | 2019-03-01 | 2022-02-11 | 中国疾病预防控制中心传染病预防控制所 | Application of Mycobacterial Ku protein |
| CN116064853A (en) * | 2022-08-17 | 2023-05-05 | 浙江迪谱诊断技术有限公司 | A kind of test kit and its application |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69230305T2 (en) * | 1991-08-15 | 2000-07-20 | F. Hoffmann-La Roche Ag, Basel | Pair of primers for the detection of Mycobacterium |
| FR2709310B1 (en) * | 1993-07-23 | 1995-09-29 | Bio Merieux | Nucleotide fragment of 23S ribosomal RNA from mycobacteria, probes and primers, reagent and method of detection. |
| US5925518A (en) * | 1995-05-19 | 1999-07-20 | Akzo Nobel N.V. | Nucleic acid primers for amplification of a mycobacteria RNA template |
| CN1542142A (en) * | 1999-05-29 | 2004-11-03 | Sj高技术株式会社 | Oligonucleotide for detection and identification of mycobacteria |
-
2000
- 2000-09-15 KR KR10-2000-0054166A patent/KR100433260B1/en not_active Expired - Fee Related
-
2001
- 2001-08-14 WO PCT/KR2001/001385 patent/WO2002022872A1/en not_active Ceased
- 2001-08-14 AU AU2001278813A patent/AU2001278813A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR100433260B1 (en) | 2004-05-24 |
| KR20020021453A (en) | 2002-03-21 |
| WO2002022872A1 (en) | 2002-03-21 |
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