[go: up one dir, main page]

AU2001275091A1 - Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides - Google Patents

Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides

Info

Publication number
AU2001275091A1
AU2001275091A1 AU2001275091A AU7509101A AU2001275091A1 AU 2001275091 A1 AU2001275091 A1 AU 2001275091A1 AU 2001275091 A AU2001275091 A AU 2001275091A AU 7509101 A AU7509101 A AU 7509101A AU 2001275091 A1 AU2001275091 A1 AU 2001275091A1
Authority
AU
Australia
Prior art keywords
synthetic
dna
double
oligonucleotides
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001275091A
Other languages
English (en)
Inventor
John T. Mulligan
John C Tabone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blue Heron Biotechnology Inc
Original Assignee
Blue Heron Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blue Heron Biotechnology Inc filed Critical Blue Heron Biotechnology Inc
Publication of AU2001275091A1 publication Critical patent/AU2001275091A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2001275091A 2000-06-02 2001-06-01 Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides Abandoned AU2001275091A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20875300P 2000-06-02 2000-06-02
US60208753 2000-06-02
PCT/US2001/017695 WO2001094366A1 (fr) 2000-06-02 2001-06-01 Methode visant a ameliorer la fidelite sequentielle des oligonucleotides synthetiques bicatenaires

Publications (1)

Publication Number Publication Date
AU2001275091A1 true AU2001275091A1 (en) 2001-12-17

Family

ID=22775909

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001275091A Abandoned AU2001275091A1 (en) 2000-06-02 2001-06-01 Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides

Country Status (6)

Country Link
US (1) US6664112B2 (fr)
EP (1) EP1287010A1 (fr)
JP (1) JP2004509609A (fr)
AU (1) AU2001275091A1 (fr)
CA (1) CA2410440A1 (fr)
WO (1) WO2001094366A1 (fr)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057924A1 (fr) * 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Procede de correction d'epreuve, de suppression d'erreur et de ligation pour la synthese de sequences de polynucleotides haute fidelite
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
ES2534744T3 (es) 2004-04-22 2015-04-28 Grifols Therapeutics Inc. Plasmina modificada de forma recombinante
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
EP1812598A1 (fr) * 2004-10-18 2007-08-01 Codon Devices, Inc. Procedes d'assemblage de polynucleotides synthetiques de haute fidelite
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20090087840A1 (en) * 2006-05-19 2009-04-02 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (fr) 2006-08-31 2008-03-06 Codon Devices, Inc. Assemblage itératif d'acides nucléiques utilisant l'activation de caractères codés par vecteurs
CA2707266C (fr) 2007-11-29 2013-12-31 Talecris Biotherapeutics, Inc. Plasmine modifiee de maniere recombinante
EP2297317A4 (fr) 2008-06-04 2011-12-07 Talecris Biotherapeutics Inc Composition, méthode et nécessaire pour la préparation de plasmine
CN102171368B (zh) * 2008-10-01 2017-06-20 皇家飞利浦电子股份有限公司 在载体上固定化核酸的方法
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
PT2403865E (pt) 2009-03-03 2015-11-18 Grifols Therapeutics Inc Métodos de preparação de plasminogénio
WO2011056872A2 (fr) 2009-11-03 2011-05-12 Gen9, Inc. Procédés et dispositifs microfluidiques pour la manipulation de gouttelettes dans un ensemble polynucléotidique haute fidélité
MX2012005527A (es) 2009-11-13 2012-08-08 Grifols Therapeutics Inc Preparaciones que contienen el factor de von willebrand (fvw) procedimientos, kits y aplicaciones relacionados con las mismas.
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP2637780B1 (fr) 2010-11-12 2022-02-09 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP3954770A1 (fr) 2011-08-26 2022-02-16 Gen9, Inc. Compositions et procédés pour ensemble haute fidélité d'acides nucléiques
AU2012340501A1 (en) 2011-12-19 2013-07-11 Grifols, S.A. Compositions, methods, and kits for preparing sialylated recombinant proteins
US8691960B2 (en) 2012-02-29 2014-04-08 Duke University Oxidoreductases for enantioselective reactions
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (fr) 2012-04-24 2013-10-31 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
EP4610368A3 (fr) 2013-08-05 2025-11-05 Twist Bioscience Corporation Banques de gènes synthétisés de novo
WO2016126882A1 (fr) 2015-02-04 2016-08-11 Twist Bioscience Corporation Procédés et dispositifs pour assemblage de novo d'acide oligonucléique
WO2016126987A1 (fr) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions et méthodes d'assemblage de gène synthétique
WO2016172377A1 (fr) 2015-04-21 2016-10-27 Twist Bioscience Corporation Dispositifs et procédés pour la synthèse de banques d'acides oligonucléiques
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
KR20250053972A (ko) 2015-09-22 2025-04-22 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
WO2017095958A1 (fr) 2015-12-01 2017-06-08 Twist Bioscience Corporation Surfaces fonctionnalisées et leur préparation
CN109996876A (zh) 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
JP7169975B2 (ja) 2016-12-16 2022-11-11 ツイスト バイオサイエンス コーポレーション 免疫シナプスの変異体ライブラリーおよびその合成
EP3586255B1 (fr) 2017-02-22 2025-01-15 Twist Bioscience Corporation Stockage de données reposant sur un acide nucléique
EP3595674A4 (fr) 2017-03-15 2020-12-16 Twist Bioscience Corporation Banques de variants de la synapse immunologique et leur synthèse
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (fr) 2017-06-12 2018-12-20 Twist Bioscience Corporation Méthodes d'assemblage d'acides nucléiques continus
JP2020536504A (ja) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション Gpcr結合タンパク質およびその合成
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
CN120485344A (zh) 2018-01-04 2025-08-15 特韦斯特生物科学公司 基于dna的数字信息存储
JP2021526366A (ja) 2018-05-18 2021-10-07 ツイスト バイオサイエンス コーポレーション 核酸ハイブリダイゼーションのためのポリヌクレオチド、試薬、および方法
WO2020139871A1 (fr) 2018-12-26 2020-07-02 Twist Bioscience Corporation Synthèse de novo polynucléotidique hautement précise
SG11202109322TA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for glp1 receptor
WO2020176680A1 (fr) 2019-02-26 2020-09-03 Twist Bioscience Corporation Banques d'acides nucléiques variants pour l'optimisation d'anticorps
CA3144644A1 (fr) 2019-06-21 2020-12-24 Twist Bioscience Corporation Assemblage de sequences d'acide nucleique base sur des code-barres
CA3155630A1 (fr) 2019-09-23 2021-04-01 Twist Bioscience Corporation Bibliotheques d'acides nucleiques variants pour des anticorps a domaine unique
AU2020356471A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
WO2022221853A1 (fr) 2021-04-13 2022-10-20 Elegen Corp. Procédés et compositions pour le clonage acellulaire

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652639A (en) 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
US6030799A (en) * 1985-01-14 2000-02-29 Washington Research Foundation Immunoassays for detecting chlamydial antigens or antibodies thereto using recombinant or synthetic major outer membrane protein polypeptides as substitute antigens
US5795976A (en) 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
US5668268A (en) * 1995-11-27 1997-09-16 Hybridon, Inc. Passivated polymer supports for nucleic acid synthesis
EP1042503A4 (fr) 1997-10-31 2002-01-16 Transgenomic Inc Procede de detection d'adn mutant par mipc et pcr
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Also Published As

Publication number Publication date
JP2004509609A (ja) 2004-04-02
US20020077471A1 (en) 2002-06-20
EP1287010A1 (fr) 2003-03-05
CA2410440A1 (fr) 2001-12-13
US6664112B2 (en) 2003-12-16
WO2001094366A1 (fr) 2001-12-13

Similar Documents

Publication Publication Date Title
US6664112B2 (en) Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US5506212A (en) Oligonucleotides with substantially chirally pure phosphorothioate linkages
EP0655088B1 (fr) Oligonucleotides presentant des liaisons phosphorees chirales
US7615629B2 (en) Methods and compositions for the tandem synthesis of two or more oligonucleotides on the same solid support
WO2003054232A2 (fr) Procedes de suppression des oligonucleotides bicatenaires contenant des erreurs de sequençage a l'aide de proteines de reconnaissance de mesappariements
WO2003102212A9 (fr) Evolution in vitro d'arn et d'adn fonctionnels utilisant la selection electrophoretique
JP2005520547A (ja) オリゴヌクレオチド及びその類似体のための精製方法
US20250122494A1 (en) Methods for separating molecular species of guanine-rich oligonucleotides
WO2025050466A1 (fr) Désoxyribozyme et procédé de détection de l'efficacité de coiffage d'arnm
US5869236A (en) Mutation detecting method using photobridging-stabilized double-stranded DNA denaturing gradient electrophoresis
AU2003206126A1 (en) Methods for two-dimensional conformation-dependent separation of non-circular nucleic acids
KR101002499B1 (ko) 2본쇄 dna 표적 서열을 3중 나선 상호작용에 의해 정제 및 검출하는 방법
WO2003014398A2 (fr) Procedes et compositions de detection de mutations par chromatographie liquide
US7052838B2 (en) Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction
US6153742A (en) General process for the preparation of cyclic oligonucleotides
Higgins et al. The ATP analogue containing a methylene group in place of the 5′ oxygen serves as a substrate for bacteriophage T3 RNA polymerase
US20030082557A1 (en) Methods and compositions for mutation detection by liquid chromatography
Schneider et al. Recent Advances in DNA Separations: Plasmid Purification, Rapid Electrophoresis, and Affinity-Based Recovery
WO2001032924A1 (fr) Compositions, procedes de syntese et utilisation de nouvelles structures d'acides nucleiques
WO2025007112A1 (fr) Procédés d'analyse et de caractérisation d'acide ribonucléique messager (arnm) et de polyadénylation sur arnm
Ohmichi et al. A nano-circular single-stranded DNA as a novel tool for SNPs detection