[go: up one dir, main page]

AU2001273432A1 - Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix - Google Patents

Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix

Info

Publication number
AU2001273432A1
AU2001273432A1 AU2001273432A AU7343201A AU2001273432A1 AU 2001273432 A1 AU2001273432 A1 AU 2001273432A1 AU 2001273432 A AU2001273432 A AU 2001273432A AU 7343201 A AU7343201 A AU 7343201A AU 2001273432 A1 AU2001273432 A1 AU 2001273432A1
Authority
AU
Australia
Prior art keywords
protein
matrix
cell
molecules
peptide molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001273432A
Other languages
English (en)
Inventor
Robert W. Blakesley
Peter Clausen
Barbara Flynn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2001273432A1 publication Critical patent/AU2001273432A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3234—Inorganic material layers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/327—Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6065—Construction of the column body with varying cross section
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6091—Cartridges

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Water Supply & Treatment (AREA)
  • Inorganic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2001273432A 2000-07-13 2001-07-13 Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix Abandoned AU2001273432A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US21808100P 2000-07-13 2000-07-13
US60218081 2000-07-13
US27463001P 2001-03-12 2001-03-12
US60274630 2001-03-12
PCT/US2001/022080 WO2002006456A1 (fr) 2000-07-13 2001-07-13 Methodes et compositions d'extraction et d'isolation rapides de proteines et de peptides au moyen d'une matrice de lyse

Publications (1)

Publication Number Publication Date
AU2001273432A1 true AU2001273432A1 (en) 2002-01-30

Family

ID=26912535

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001273432A Abandoned AU2001273432A1 (en) 2000-07-13 2001-07-13 Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix

Country Status (7)

Country Link
US (1) US20020012982A1 (fr)
EP (1) EP1301591A4 (fr)
JP (1) JP2004504330A (fr)
AU (1) AU2001273432A1 (fr)
CA (1) CA2415713A1 (fr)
NZ (1) NZ523831A (fr)
WO (1) WO2002006456A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111818980A (zh) * 2018-03-08 2020-10-23 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8563232B2 (en) 2000-09-12 2013-10-22 Lifenet Health Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced
US20080077251A1 (en) * 1999-06-07 2008-03-27 Chen Silvia S Cleaning and devitalization of cartilage
WO2002065125A1 (fr) * 2001-02-13 2002-08-22 Invitrogen Corporation Procedes et compositions utilises dans l'isolation de macromolecules biologiques
WO2003025156A2 (fr) * 2001-09-18 2003-03-27 Affinium Pharmaceuticals, Inc. Procedes et appareils de purification
US7923431B2 (en) * 2001-12-21 2011-04-12 Ferrosan Medical Devices A/S Haemostatic kit, a method of preparing a haemostatic agent and a method of promoting haemostatis
US20050079526A1 (en) * 2002-02-20 2005-04-14 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing refolding and aggregation of biological molecules
CA2477021A1 (fr) * 2002-02-20 2003-08-28 Affinium Pharmaceuticals, Inc. Methodes et dispositifs destines a caracteriser la stabilite de molecules biologiques
US20040121445A1 (en) * 2002-07-31 2004-06-24 Fabien Marino Cell cultures
JP2006507818A (ja) 2002-11-01 2006-03-09 プロメガ　コーポレイション 細胞溶解のための組成物、使用方法、装置およびキット
CA2509914A1 (fr) * 2002-12-11 2004-06-24 Ferrosan A/S Tampons a prelevements a base de gelatine
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
CA2524393A1 (fr) * 2003-05-02 2004-11-18 Sigma-Aldrich Co. Lyse cellulaire a phase solide et plaque de saisie
EP2216415B2 (fr) * 2003-08-01 2017-01-04 Life Technologies Corporation Compositions et procédés de préparation de courtes molécules d'ARN et d'autres acides nucléiques
JP2005095160A (ja) * 2003-08-19 2005-04-14 Fuji Photo Film Co Ltd 抽出装置用ラック
WO2005072700A2 (fr) * 2004-01-30 2005-08-11 Ferrosan A/S Pulverisations et compositions hemostatiques
JP4959557B2 (ja) * 2004-07-09 2012-06-27 フェロサン メディカル デバイシーズ エイ／エス ヒアルロン酸含有止血合成剤
WO2006020868A2 (fr) * 2004-08-13 2006-02-23 New England Biolabs, Inc. Production intracellulaire d'une a nuclease
WO2006026248A1 (fr) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions et procedes faisant appel a des combinaisons de detergents zwitterioniques
WO2006034009A2 (fr) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Procede d'epuration permettant de debarrasser des biomolecules d'acides nucleiques intacts contaminants
GB0423485D0 (en) * 2004-10-22 2004-11-24 First Water Ltd Absorbent materials and articles
US20060105391A1 (en) * 2004-11-12 2006-05-18 Promega Corporation Device and method for separating molecules
US20060105349A1 (en) * 2004-11-12 2006-05-18 Promega Corporation Device and method for purification of biological materials
WO2006064512A1 (fr) * 2004-12-13 2006-06-22 Unichem Laboratories Limited Procédé de fractionnement et de stockage de protéines
US20060183889A1 (en) * 2005-01-04 2006-08-17 Bogoev Roumen A Alkyl phosphine detergent products and extraction processes
EP1841854A4 (fr) * 2005-01-27 2009-10-21 Applera Corp Dispositifs et procedes de preparation d'echantillons
GB0522193D0 (en) * 2005-10-31 2005-12-07 Axis Shield Asa Method
US8003369B2 (en) 2005-11-29 2011-08-23 Sanyo Chemical Industries, Ltd. Bacteriolytic agent
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP1939212A1 (fr) * 2006-12-20 2008-07-02 LEK Pharmaceuticals D.D. Composés organiques
EP1938756A1 (fr) * 2006-12-29 2008-07-02 Qiagen GmbH Procédure et matériaux destinés à la libération contrôlée d'une probe biologique
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
US9744043B2 (en) 2007-07-16 2017-08-29 Lifenet Health Crafting of cartilage
JP5215677B2 (ja) 2008-01-21 2013-06-19 倉敷紡績株式会社 多孔質フィルターカートリッジ
DE602008002545D1 (de) * 2008-02-01 2010-10-28 Eppendorf Ag Kulturplatte mit Klappe zur seitlichen Belüftung
US8642831B2 (en) * 2008-02-29 2014-02-04 Ferrosan Medical Devices A/S Device for promotion of hemostasis and/or wound healing
US20110044968A1 (en) * 2008-03-10 2011-02-24 Pharmal N Corporation Compositions for treatment with metallopeptidases, methods of making and using the same
BRPI0903708A2 (pt) * 2008-06-13 2015-06-30 Yamaha Motor Co Ltd Motor de combustão interna, veículo, embarcação marítima, e método de purificação de gás de descarga para motor de combustão interna
KR101005924B1 (ko) * 2008-06-27 2011-01-06 포항공과대학교 산학협력단 핵산 추출 장치
WO2012016720A1 (fr) * 2010-08-05 2012-02-09 Vibod Gmbh Colonnes inédites utilisables en vue de l'incubation et de l'isolement d'échantillons chimiques et/ou biologiques
IL267585B (en) * 2011-12-22 2022-09-01 Genentech Inc Ion exchange membrane chromatography
CA2865349C (fr) 2012-03-06 2021-07-06 Ferrosan Medical Devices A/S Recipient mis sous pression contenant de la pate hemostatique
EP2825216B1 (fr) 2012-06-12 2015-08-19 Ferrosan Medical Devices A/S Composition hémostatique sèche
BR112015005937A2 (pt) 2012-09-17 2017-07-04 Grace W R & Co meios e dispositivos cromatográficos
RU2015114177A (ru) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. Функционализированный, состоящий из частиц материал носителя и способы его получения и применения
AU2014283170B2 (en) 2013-06-21 2017-11-02 Ferrosan Medical Devices A/S Vacuum expanded dry composition and syringe for retaining same
KR101507234B1 (ko) * 2013-07-17 2015-03-31 로레알 생분자 추출기 및 생분자 추출방법
RU2678592C1 (ru) 2013-12-11 2019-01-30 Ферросан Медикал Дивайсиз А/С Сухая композиция, содержащая компонент, улучшающий экструзию
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
WO2015168342A1 (fr) * 2014-04-29 2015-11-05 Accudx Corporation Nouvelle matrice d'affinité et dispositifs pour l'isolement et la purification d'arn et d'adn pour des dispositifs moléculaires de point de soin
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
BR112017007466B1 (pt) 2014-10-13 2021-03-02 Ferrosan Medical Devices A/S método para preparar uma composição seca, método para reconstituir a composição seca, pasta, composição seca, recipiente, kit homeostático, e, uso de uma composição seca
BR112017013565B1 (pt) 2014-12-24 2021-12-28 Ferrosan Medical Devices A/S Seringa para retenção e mistura de primeira e segunda substâncias
PL3302784T3 (pl) 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
RU2717356C2 (ru) 2015-07-03 2020-03-23 Ферросан Медикал Дивайсиз А/С Шприц для удерживания вакуума в состоянии хранения
CN104928176B (zh) * 2015-07-16 2017-03-08 中南大学湘雅医院 含组织固定及多层滤过装置的原代肝细胞分离系统及方法
CN104946528A (zh) * 2015-07-16 2015-09-30 中南大学湘雅医院 一种原代肝组织细胞分离系统及分离方法
WO2017069781A1 (fr) * 2015-10-23 2017-04-27 Life Technologies Corporation Système et procédé à base de filtre pour la capture et la lyse efficaces de cellules en suspension
US9758755B2 (en) 2015-10-23 2017-09-12 Life Technologies Corporation Filter-based method for efficient capture of lysis of suspended cells
US10863737B2 (en) * 2017-05-22 2020-12-15 Drobot Biotechnology Limited Company Culture container, and system and method of transferring a cultured organism between culture containers
CN107058075B (zh) * 2017-06-20 2023-10-20 商丘师范学院 一种植物细胞原生质体纯化仪及纯化方法
CN107880114B (zh) * 2017-12-13 2020-12-29 国药肽谷有限公司 一种牛骨胶原蛋白肽生产设备
KR20210008479A (ko) 2018-05-09 2021-01-22 훼로산 메디칼 디바이스 에이/에스 지혈 조성물을 제조하는 방법
CN112188862A (zh) 2018-05-22 2021-01-05 斯特雷拉生物技术股份有限公司 乙烯受体生物传感器
JP2023517157A (ja) 2020-03-09 2023-04-24 イルミナ，インコーポレイテッド ポリヌクレオチドを配列決定するための方法
US20240263229A1 (en) 2021-12-16 2024-08-08 Illumina, Inc. Hybrid clustering
EP4448797A1 (fr) 2021-12-17 2024-10-23 Illumina, Inc. Hybridation orthogonale
EP4493721A1 (fr) 2022-03-15 2025-01-22 Illumina, Inc. Séquençage d'échantillons et d'indices parallèles
WO2023175037A2 (fr) 2022-03-15 2023-09-21 Illumina, Inc. Séquençage simultané de brins de complément avant et inverse sur des polynucléotides séparés pour la détection de méthylation
US20230348973A1 (en) 2022-03-31 2023-11-02 Illumina Cambridge Limited Paired-end re-synthesis using blocked p5 primers
EP4590854A1 (fr) 2022-09-19 2025-07-30 Illumina, Inc. Polymères déformables comprenant des amorces immobilisées
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
WO2024123917A1 (fr) 2022-12-07 2024-06-13 Illumina, Inc. Regroupement monoclonal par exclusion stérique d'adn double brin avec ensemencement à motifs
CN116606897B (zh) * 2023-06-05 2023-11-21 意润健康产业（广州）有限公司 一种基于超滤技术的高纯度骨肽及其酶解制备系统
WO2024256581A1 (fr) 2023-06-14 2024-12-19 Illumina, Inc. Identification de cytosines modifiées
WO2024256580A1 (fr) 2023-06-14 2024-12-19 Illumina, Inc. Séquençage simultané avec des anneaux spatialement séparés
CN117074590B (zh) * 2023-08-25 2025-10-31 中国科学院生态环境研究中心 凝胶电泳耦合液相色谱-质谱联用的dna修饰检测方法
WO2025062001A1 (fr) 2023-09-20 2025-03-27 Illumina, Inc. Séquençage optimisé d'acides nucléiques
WO2025062002A1 (fr) 2023-09-20 2025-03-27 Illumina, Inc. Séquençage simultané à l'aide d'une traduction de coupure simple brin
WO2025072683A1 (fr) 2023-09-29 2025-04-03 Illumina, Inc. Supports solides et procédés pour l'amplification par invasion de brin
WO2025137479A1 (fr) 2023-12-22 2025-06-26 Illumina, Inc. Cellules d'écoulement réutilisables et leurs procédés d'utilisation pour le séquençage d'acides nucléiques

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2114748A (en) * 1934-05-09 1938-04-19 Firm Jenaer Glaswerk Schott & Method of making porous filter bodies of particles of glass
US3433782A (en) * 1965-12-27 1969-03-18 Miles Lab Separation and recovery of oligonucleotides
BE757291A (fr) * 1969-10-10 1971-04-09 Smith Kline French Lab Complexe antiviral de arn et d'un polysaccharide
BE793826A (fr) * 1972-10-24 1973-07-10 Dainippon Pharmaceutical Co Procede de purification d'une enzyme de cytolyse
US3935111A (en) * 1973-04-06 1976-01-27 Bentley Laboratories, Inc. Device for removing blood microemboli
US3925600A (en) * 1975-01-20 1975-12-09 Bell Telephone Labor Inc Line switching system for digital data
NO760938L (fr) * 1975-03-22 1976-09-23 Biotest Serum Institut Gmbh
US4303530A (en) * 1977-10-26 1981-12-01 Medical Incorporated Blood filter
JPS5598912A (en) * 1979-01-23 1980-07-28 Toray Ind Inc Glucose-isomerizing fiber and its production
US4491660A (en) * 1980-01-10 1985-01-01 Abbott Laboratories Matrix polymers for binding endotoxins
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
US4734192A (en) * 1982-07-01 1988-03-29 Millipore Corporation Multiwell membrane filtration apparatus
US4427580A (en) * 1982-09-01 1984-01-24 Cornell Research Foundation, Inc. Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
US6492107B1 (en) * 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
WO1986005803A1 (fr) * 1985-03-30 1986-10-09 Marc Ballivet Procede d'obtention d'adn, arn, peptides, polypeptides ou proteines, par une technique de recombinaison d'adn
US5091309A (en) * 1986-01-16 1992-02-25 Washington University Sindbis virus vectors
US6027750A (en) * 1986-09-04 2000-02-22 Gautsch; James Systems and methods for the rapid isolation of nucleic acids
US5750338A (en) * 1986-10-23 1998-05-12 Amoco Corporation Target and background capture methods with amplification for affinity assays
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5422251A (en) * 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4833239A (en) * 1987-01-29 1989-05-23 Genetics Institute, Inc. Method for the isolation and purification of DNA molecules
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US4973551A (en) * 1988-01-15 1990-11-27 Merck & Co., Inc. Vector for the expression of fusion proteins and protein immunogens
US4921952A (en) * 1988-01-21 1990-05-01 The United States Of America As Represented By The United States Department Of Energy Nucleic acid isolation process
US5496562A (en) * 1988-10-05 1996-03-05 Flinders Technologies Pty Ltd Solid medium and method for DNA storage
US5807527A (en) * 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US5756126A (en) * 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US5217879A (en) * 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
US4948561A (en) * 1989-02-09 1990-08-14 Eastman Kodak Company Multiple level filter device and kit containing same
US5703055A (en) * 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
DE68914689T2 (de) * 1989-05-22 1994-10-27 Genetics Inst Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsäure und dadurch verbessertes verfahren.
US5166057A (en) * 1989-08-28 1992-11-24 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand rna virus expression-systems
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5458878A (en) * 1990-01-02 1995-10-17 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services P. exotoxin fusio proteins have COOHG220101al alterations which increase cytotoxicity
US5652141A (en) * 1990-10-26 1997-07-29 Oiagen Gmbh Device and process for isolating nucleic acids from cell suspension
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
EP0555798B1 (fr) * 1992-02-13 1999-05-06 Becton, Dickinson and Company Célite hydrate et purification d'ADN
US5401632A (en) * 1992-07-16 1995-03-28 Wisconsin Alumni Research Foundation Triple helix purification and sequencing
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
AU689815B2 (en) * 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
US6015686A (en) * 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
DE4403940A1 (de) * 1994-02-08 1995-08-10 Genomed Molekularbiologische U Chromatographiematerial
US5532154A (en) * 1994-03-21 1996-07-02 Research Development Foundation Mutated alpha virus
US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5601711A (en) * 1994-10-31 1997-02-11 Gelman Sciences Inc. Selective separation filter device
US5792462A (en) * 1995-05-23 1998-08-11 University Of North Carolina At Chapel Hill Alphavirus RNA replicon systems
CA2223896A1 (fr) * 1995-06-08 1996-12-27 Robert Hugh Don Procede et appareil d'extraction d'adn
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5833860A (en) * 1995-08-28 1998-11-10 Millipore Investment Holdings Limited Centrifugal adsorptive sample preparation device and method
DE19612650C1 (de) * 1996-04-02 1997-07-31 Evotec Biosystems Gmbh Verfahren zur Fluoreszenzmarkierung von Molekülen
US5665247A (en) * 1996-09-16 1997-09-09 Whatman Inc. Process for sealing microplates utilizing a thin polymeric film
US6048457A (en) * 1997-02-26 2000-04-11 Millipore Corporation Cast membrane structures for sample preparation
US5970782A (en) * 1997-05-16 1999-10-26 Life Technologies, Inc. Gradient filtration apparatus
US6268133B1 (en) * 1997-06-25 2001-07-31 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6103195A (en) * 1997-08-08 2000-08-15 Shukla; Ashok K. Micro-volume spin columns for sample preparation
US6221655B1 (en) * 1998-08-01 2001-04-24 Cytosignal Spin filter assembly for isolation and analysis
EP1141234A4 (fr) * 1999-01-06 2004-05-19 Invitrogen Corp Procedes et compositions permettant d'isoler des molecules d'acide nucleique
GB2347077A (en) * 1999-02-27 2000-08-30 Tsay Jenn Long Combination desk and chair
EP1173744A4 (fr) * 1999-03-02 2002-10-16 Qualigen Inc Procedes et appareil de separation de fluides biologiques
NZ524065A (en) * 2000-07-18 2005-02-25 Invitrogen Corp Device and methods for subdividing and filtering gel material and extracting molecules therefrom
JP2004518421A (ja) * 2000-12-12 2004-06-24 インビトロジェン コーポレイション 固体マトリックスから核酸分子を解放させるための組成物および方法
WO2002065125A1 (fr) * 2001-02-13 2002-08-22 Invitrogen Corporation Procedes et compositions utilises dans l'isolation de macromolecules biologiques

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111818980A (zh) * 2018-03-08 2020-10-23 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂

Also Published As

Publication number Publication date
CA2415713A1 (fr) 2002-01-24
JP2004504330A (ja) 2004-02-12
EP1301591A4 (fr) 2004-05-26
EP1301591A1 (fr) 2003-04-16
NZ523831A (en) 2005-10-28
WO2002006456A1 (fr) 2002-01-24
US20020012982A1 (en) 2002-01-31

Similar Documents

Publication Publication Date Title
US20020012982A1 (en) Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
AU740145B2 (en) Endotoxin reduction in nucleic acid purification
AU774810B2 (en) Mixed-bed solid phase and its use in the isolation of nucleic acids
KR100623184B1 (ko) 임의의 복잡한 출발물질로부터 핵산을 분리하기 위한배합물과 방법 그리고 복합한 유전자 분석법
US6787307B1 (en) Lysate clearance and nucleic acid isolation using silanized silica matrices
JP4175670B2 (ja) 固体相核酸の単離
JP3115324B2 (ja) 核酸を単離する装置及び方法
JP2564335B2 (ja) 生体試料から核酸を単離し精製する方法
US9410145B2 (en) Method for the isolation of nucleic acids
JPH08501321A (ja) クロマトグラフィーによる核酸混合物の精製分離法
US7319021B2 (en) Cell lysis composition, methods of use, apparatus and kit
EP1173559A1 (fr) Procede de purification d'acides nucleiques a l'aide de carbure de silicium
JP2006523463A (ja) 固体支持体を使用してｒｎａを精製するための、組成物および方法
JP2004501054A (ja) 核酸の単離
WO2000040697A1 (fr) Procedes et compositions permettant d'isoler des molecules d'acide nucleique
AU2002225942B2 (en) Lysate clearance and nucleic acid isolation using silanized silica matrices
JP2003524157A (ja) クロマトグラフィー材料及びその使用方法
CA2315257A1 (fr) Procede d'isolement d'acides nucleiques a chaine courte et a chaine longue
JP2008506654A (ja) 陽イオン洗浄剤を使用した核酸を洗浄および単離するための方法
MXPA98007681A (en) Methods of isolating biological target materials using silica magnetic particles

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application