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AU2001268273A1 - Mutant proteinase with reduced self-cleavage activity and method of purification - Google Patents

Mutant proteinase with reduced self-cleavage activity and method of purification

Info

Publication number
AU2001268273A1
AU2001268273A1 AU2001268273A AU6827301A AU2001268273A1 AU 2001268273 A1 AU2001268273 A1 AU 2001268273A1 AU 2001268273 A AU2001268273 A AU 2001268273A AU 6827301 A AU6827301 A AU 6827301A AU 2001268273 A1 AU2001268273 A1 AU 2001268273A1
Authority
AU
Australia
Prior art keywords
purification
cleavage activity
reduced self
mutant proteinase
proteinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001268273A
Other languages
English (en)
Inventor
Robert T. Batey
Jennifer A Doudna
Louise J. Lucast
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of AU2001268273A1 publication Critical patent/AU2001268273A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22044—Nuclear-inclusion-a endopeptidase (3.4.22.44)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2001268273A 2000-06-15 2001-06-11 Mutant proteinase with reduced self-cleavage activity and method of purification Abandoned AU2001268273A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21153500P 2000-06-15 2000-06-15
US60211535 2000-06-15
PCT/US2001/018620 WO2001096539A2 (fr) 2000-06-15 2001-06-11 Proteinase mutante presentant une activite auto-clivante reduite et procede de purification

Publications (1)

Publication Number Publication Date
AU2001268273A1 true AU2001268273A1 (en) 2001-12-24

Family

ID=22787327

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001268273A Abandoned AU2001268273A1 (en) 2000-06-15 2001-06-11 Mutant proteinase with reduced self-cleavage activity and method of purification

Country Status (4)

Country Link
US (5) US7494786B2 (fr)
AU (1) AU2001268273A1 (fr)
CA (1) CA2435381C (fr)
WO (1) WO2001096539A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001096539A2 (fr) 2000-06-15 2001-12-20 Yale University Proteinase mutante presentant une activite auto-clivante reduite et procede de purification
US6969757B2 (en) 2001-01-26 2005-11-29 Syngenta Participations Ag Differential labeling for quantitative analysis of complex protein mixtures
US7166436B2 (en) 2001-01-26 2007-01-23 Syngenta Participations, Ag Differential labeling for quantitative analysis of complex protein mixtures
WO2011038219A1 (fr) * 2009-09-24 2011-03-31 Promega Corporation Protéases ayant une stabilité, une solubilité et une activité améliorées
CN101864407B (zh) * 2010-06-18 2016-02-10 安徽农业大学 Tev蛋白酶突变体及编码基因及其应用
WO2012011621A1 (fr) * 2010-07-23 2012-01-26 Gwangju Institute Of Science And Technology Clairance d'amyloïde bêta
KR101536963B1 (ko) * 2013-09-17 2015-07-16 재단법인 전주생물소재연구소 식물에서 tev 프로테아제를 대량생산하는 방법 및 상기 방법에 의해 제조된 tev 프로테아제
US11661599B1 (en) 2017-12-14 2023-05-30 National Technology & Engineering Solutions Of Sandia, Llc CRISPR-Cas based system for targeting single-stranded sequences

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
WO2001096539A2 (fr) 2000-06-15 2001-12-20 Yale University Proteinase mutante presentant une activite auto-clivante reduite et procede de purification

Also Published As

Publication number Publication date
US8470575B2 (en) 2013-06-25
US20040072179A1 (en) 2004-04-15
US8969062B2 (en) 2015-03-03
US20080318273A1 (en) 2008-12-25
US20130337503A1 (en) 2013-12-19
WO2001096539A3 (fr) 2002-05-16
CA2435381C (fr) 2013-09-17
US8206968B2 (en) 2012-06-26
US20150152403A1 (en) 2015-06-04
WO2001096539A2 (fr) 2001-12-20
US20120252096A1 (en) 2012-10-04
CA2435381A1 (fr) 2001-12-20
US7494786B2 (en) 2009-02-24

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