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AU2001265394A1 - Method and apparatus for purifying nucleic acids - Google Patents

Method and apparatus for purifying nucleic acids

Info

Publication number
AU2001265394A1
AU2001265394A1 AU2001265394A AU6539401A AU2001265394A1 AU 2001265394 A1 AU2001265394 A1 AU 2001265394A1 AU 2001265394 A AU2001265394 A AU 2001265394A AU 6539401 A AU6539401 A AU 6539401A AU 2001265394 A1 AU2001265394 A1 AU 2001265394A1
Authority
AU
Australia
Prior art keywords
nucleic acids
purifying nucleic
purifying
acids
nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001265394A
Other languages
English (en)
Inventor
R. Scott Duthie
Peter Hewitt
Johan Wahlberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech Inc filed Critical Amersham Pharmacia Biotech Inc
Publication of AU2001265394A1 publication Critical patent/AU2001265394A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2001265394A 2000-06-07 2001-06-07 Method and apparatus for purifying nucleic acids Abandoned AU2001265394A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21006200P 2000-06-07 2000-06-07
US60210062 2000-06-07
PCT/US2001/018434 WO2001094574A2 (fr) 2000-06-07 2001-06-07 Procede et appareil de purification d'acides nucleiques

Publications (1)

Publication Number Publication Date
AU2001265394A1 true AU2001265394A1 (en) 2001-12-17

Family

ID=22781446

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001265394A Abandoned AU2001265394A1 (en) 2000-06-07 2001-06-07 Method and apparatus for purifying nucleic acids

Country Status (3)

Country Link
US (1) US20020040873A1 (fr)
AU (1) AU2001265394A1 (fr)
WO (1) WO2001094574A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
JP2008536478A (ja) * 2005-02-15 2008-09-11 アプレラ コーポレイション 高分子電解質被覆イオン交換粒子
EP1848793B1 (fr) * 2005-02-15 2012-06-20 Life Technologies Corporation Particules d'echange ionique revetues de polyelectrolytes
DE102007005655A1 (de) 2007-01-31 2008-08-07 Qiagen Gmbh Vorrichtung und Verfahren zur Aufreinigung von Nukleinsäuren
US8753868B2 (en) * 2008-08-04 2014-06-17 General Electric Company Method and system for selective isolation of target biological molecules in a general purpose system
JP2013528786A (ja) * 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー 核酸を単離および精製するためのクロマトグラフィーデバイスおよび方法
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法
EP2395082A1 (fr) 2010-06-14 2011-12-14 QIAGEN GmbH Extraction d'acides nucléiques à partir d'échantillons inclus dans de la cire
EP2593770B1 (fr) * 2010-07-14 2020-05-06 Qiagen GmbH Dispositif d'isolement et/ou de purification de biomolécules
WO2012007504A1 (fr) * 2010-07-14 2012-01-19 Qiagen Gmbh Nouveau dispositif pour le traitement des liquides
JP2013533979A (ja) * 2010-07-14 2013-08-29 キアゲン ゲーエムベーハー 新規の貯蔵、収集、または単離デバイス

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
JPH04360686A (ja) * 1991-06-04 1992-12-14 Tosoh Corp Ｄｎａの精製方法
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
WO2000066723A1 (fr) * 1999-05-04 2000-11-09 Millipore Corporation Procede d'ultrafiltration
US6498240B1 (en) * 1999-09-17 2002-12-24 Millipore Corporation Method for sequencing reaction cleanup by constant pressure diffential ultrafiltration

Also Published As

Publication number Publication date
WO2001094574A3 (fr) 2002-06-20
US20020040873A1 (en) 2002-04-11
WO2001094574A2 (fr) 2001-12-13

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