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AU2001263286A1 - High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein - Google Patents

High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein

Info

Publication number
AU2001263286A1
AU2001263286A1 AU2001263286A AU6328601A AU2001263286A1 AU 2001263286 A1 AU2001263286 A1 AU 2001263286A1 AU 2001263286 A AU2001263286 A AU 2001263286A AU 6328601 A AU6328601 A AU 6328601A AU 2001263286 A1 AU2001263286 A1 AU 2001263286A1
Authority
AU
Australia
Prior art keywords
distinguishing
sample
systems
throughput methods
multiple molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001263286A
Other languages
English (en)
Inventor
Yinfa Ma
Michael Shortreed
Edward S. Yeung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iowa State University Research Foundation Inc ISURF
Original Assignee
Iowa State University Research Foundation Inc ISURF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iowa State University Research Foundation Inc ISURF filed Critical Iowa State University Research Foundation Inc ISURF
Publication of AU2001263286A1 publication Critical patent/AU2001263286A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AU2001263286A 2000-05-19 2001-05-18 High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein Abandoned AU2001263286A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20583200P 2000-05-19 2000-05-19
US60/205,832 2000-05-19
PCT/US2001/016187 WO2001090748A2 (fr) 2000-05-19 2001-05-18 Procedes a rendement eleve servant a distinguer individuellement au moins une molecule, dans un echantillon comprenant plusieurs molecules, et systemes concus a cet effet

Publications (1)

Publication Number Publication Date
AU2001263286A1 true AU2001263286A1 (en) 2001-12-03

Family

ID=22763824

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001263286A Abandoned AU2001263286A1 (en) 2000-05-19 2001-05-18 High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein

Country Status (3)

Country Link
US (1) US20020167665A1 (fr)
AU (1) AU2001263286A1 (fr)
WO (1) WO2001090748A2 (fr)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124592A1 (en) * 2001-10-24 2003-07-03 Bioprofile, Llc Methods for detecting genetic haplotypes by interaction with probes
WO2005019419A2 (fr) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection de molecules individuelles de polypeptides et de polynucleotides
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
SE526643C2 (sv) * 2004-02-20 2005-10-18 Anders Hedqvist Metod för att undvika blekning vid tillämpning av fluorescenskorrelationsspektroskopi
JP4499476B2 (ja) * 2004-05-25 2010-07-07 オリンパス株式会社 分光画像入力装置及びそれを備えた光学装置
EP1805500A4 (fr) * 2004-09-28 2008-05-07 Singulex Inc Systeme et methode d'analyse d'echantillons
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
EP2016394A4 (fr) * 2006-04-04 2013-04-24 Singulex Inc Procédés et compositions d'analyse extrêmement sensible de marqueurs et de détection de molécules
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
CN101946180B (zh) 2007-12-19 2013-11-13 神谷来克斯公司 单分子检测用扫描分析器和使用方法
CN102016552A (zh) * 2008-03-05 2011-04-13 神谷来克斯公司 用于分子的高灵敏性检测的方法和组合物
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
JP2012524904A (ja) * 2009-04-21 2012-10-18 ユニバーシティ オブ ユタ リサーチ ファウンデーション 手術時イメージングまたはセンチネルリンパ節バイオプシー用の発光ダイ
AU2010259022B2 (en) * 2009-06-08 2016-05-12 Singulex, Inc. Highly sensitive biomarker panels
US9207205B2 (en) * 2009-12-17 2015-12-08 The Curators Of The University Of Missouri Method and apparatus for cancer screening
WO2011140484A1 (fr) 2010-05-06 2011-11-10 Singulex, Inc Méthodes de diagnostic, de classification et de prédiction du risque de développement d'une polyarthrite rhumatoïde et identification des sujets répondant à un traitement
DE102012202730B4 (de) * 2012-02-22 2025-07-24 Carl Zeiss Microscopy Gmbh Wellenlängenselektive und örtlich hochauflösende Fluoreszenzmikroskopie
EA021859B1 (ru) * 2012-05-11 2015-09-30 Государственное Научное Учреждение "Институт Тепло- И Массообмена Имени А.В. Лыкова Национальной Академии Наук Беларуси" Устройство для определения магнитных свойств диамагнитных и парамагнитных, в том числе биологических, частиц
CN103995038A (zh) * 2014-05-28 2014-08-20 天津大学 一种毛细管电泳筛选棘白菌素类药物的方法
EP3574307A4 (fr) * 2017-01-26 2020-10-21 Azure Biosystems, Inc. Dispositifs et procédés d'imagerie de biomolécules
US20240044798A1 (en) * 2020-12-21 2024-02-08 Hitachi High-Tech Corporation Flow cell for analysis of nucleic acid and device for analysis of nucleic acid
WO2024121854A1 (fr) * 2022-12-08 2024-06-13 Technion Research & Development Foundation Limited Détection à paramètres multiples dans un nanocanal

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3766048A (en) * 1972-11-24 1973-10-16 Univ Illinois Analysis of polymer mixtures in solution utilizing electrophoretic light scattering apparatus
DD140504A1 (de) * 1978-03-10 1980-03-05 Juergen Altmann Anordnung zur geschwindigkeitsmessung von bildpunkten eines videobildes
JPS6266153A (ja) * 1985-09-18 1987-03-25 Fuji Photo Film Co Ltd 電気泳動用媒体
US5190629A (en) * 1987-07-16 1993-03-02 Fuji Photo Film Co., Ltd. Electrophoresis medium membrane
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
EP0391674B1 (fr) * 1989-04-05 1996-03-20 New York University Procédé de caractérisation de particules
US5215883A (en) * 1990-07-09 1993-06-01 The Research Foundation Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching
AU4850793A (en) * 1992-09-14 1994-04-12 Purdue Research Foundation Electrophoretically mediated chemical analysis
US5538613A (en) * 1993-10-26 1996-07-23 Genesys Technologies, Inc. Electrophoresis analyzer
DE4438833A1 (de) * 1994-10-31 1996-05-02 Bayer Ag Verfahren zur analytischen Trennung von Viren
US5630924A (en) * 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US6485625B1 (en) * 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US5699157A (en) * 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
EP0854362A3 (fr) * 1997-01-16 2000-12-20 Japan Science and Technology Corporation Appareil d'électrophorèse à capillaires multiples
US5932080A (en) * 1997-05-30 1999-08-03 Bio-Rad Laboratories, Inc. Mobility-based and normalized capillary electrophoresis
US5998796A (en) * 1997-12-22 1999-12-07 Spectrumedix Corporation Detector having a transmission grating beam splitter for multi-wavelength sample analysis
WO1999040410A1 (fr) * 1998-02-06 1999-08-12 The Cleveland Clinic Foundation Systeme et procede permettant de determiner des caracteristiques de particules
GB2335981B (en) * 1998-03-31 2000-06-21 Zetatronics Ltd Rapid method for detecting micro-organisms and evaluating antimicrobial activity
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6438279B1 (en) * 1999-01-07 2002-08-20 Cornell Research Foundation, Inc. Unitary microcapiliary and waveguide structure and method of fabrication
US6592821B1 (en) * 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6309886B1 (en) * 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
US6630063B1 (en) * 1999-10-06 2003-10-07 I. Reich Family Limited Partnership Uniform laser excitation and detection in capillary array electrophoresis system and method
WO2001069211A1 (fr) * 2000-03-16 2001-09-20 Spectrumedix Corporation Lecteur de jeux d'echantillons a longueurs d'onde multiples pour dosage biologique

Also Published As

Publication number Publication date
US20020167665A1 (en) 2002-11-14
WO2001090748A2 (fr) 2001-11-29
WO2001090748A3 (fr) 2003-02-06

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