AU2001260714A1 - Method of analyzing components in biological samples - Google Patents
Method of analyzing components in biological samplesInfo
- Publication number
- AU2001260714A1 AU2001260714A1 AU2001260714A AU6071401A AU2001260714A1 AU 2001260714 A1 AU2001260714 A1 AU 2001260714A1 AU 2001260714 A AU2001260714 A AU 2001260714A AU 6071401 A AU6071401 A AU 6071401A AU 2001260714 A1 AU2001260714 A1 AU 2001260714A1
- Authority
- AU
- Australia
- Prior art keywords
- biological samples
- analyzing components
- calixarene
- vldl
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000012472 biological sample Substances 0.000 title 1
- 108010062497 VLDL Lipoproteins Proteins 0.000 abstract 3
- 108010010234 HDL Lipoproteins Proteins 0.000 abstract 2
- 102000015779 HDL Lipoproteins Human genes 0.000 abstract 2
- 108010013563 Lipoprotein Lipase Proteins 0.000 abstract 2
- 102000043296 Lipoprotein lipases Human genes 0.000 abstract 2
- 102000000019 Sterol Esterase Human genes 0.000 abstract 2
- 108010055297 Sterol Esterase Proteins 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- VTJUKNSKBAOEHE-UHFFFAOYSA-N calixarene Chemical compound COC(=O)COC1=C(CC=2C(=C(CC=3C(=C(C4)C=C(C=3)C(C)(C)C)OCC(=O)OC)C=C(C=2)C(C)(C)C)OCC(=O)OC)C=C(C(C)(C)C)C=C1CC1=C(OCC(=O)OC)C4=CC(C(C)(C)C)=C1 VTJUKNSKBAOEHE-UHFFFAOYSA-N 0.000 abstract 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- 102000009027 Albumins Human genes 0.000 abstract 1
- 108010088751 Albumins Proteins 0.000 abstract 1
- 108090001030 Lipoproteins Proteins 0.000 abstract 1
- 102000004895 Lipoproteins Human genes 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 235000012000 cholesterol Nutrition 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
There is provided a method for a selective assay of component, particularly cholesterol, in very low-density lipoprotein (VLDL) which is one of serum lipoproteins. In the assay, an enzymatic reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE) which well reacts with high-density lipoprotein (HDL) and VLDL is carried out at least in the presence of calixarene or a salt thereof. It is also carried out in the presence of one or more substance(s) selected from albumin and basic amino acids in addition to calixarene or a salt thereof.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000-171136 | 2000-06-07 | ||
| JP2000171136 | 2000-06-07 | ||
| PCT/JP2001/004721 WO2001094619A1 (en) | 2000-06-07 | 2001-06-05 | Method of analyzing components in biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001260714A1 true AU2001260714A1 (en) | 2001-12-17 |
Family
ID=18673729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001260714A Abandoned AU2001260714A1 (en) | 2000-06-07 | 2001-06-05 | Method of analyzing components in biological samples |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6986998B2 (en) |
| EP (1) | EP1288306B1 (en) |
| AT (1) | ATE446378T1 (en) |
| AU (1) | AU2001260714A1 (en) |
| DE (1) | DE60140251D1 (en) |
| WO (1) | WO2001094619A1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4456715B2 (en) * | 2000-02-28 | 2010-04-28 | 協和メデックス株式会社 | Method and reagent for measuring cholesterol in remnant-like lipoprotein |
| US6986998B2 (en) * | 2000-06-07 | 2006-01-17 | International Reagents Corporation | Method of analyzing components in biological samples |
| US7074581B2 (en) * | 2002-08-09 | 2006-07-11 | Sysmex Corporation | Reagent for assaying lipid |
| JP4527950B2 (en) * | 2002-08-09 | 2010-08-18 | シスメックス株式会社 | Lipid measuring reagent |
| EP1555327A4 (en) * | 2002-10-16 | 2006-08-02 | Kyowa Medex Co Ltd | Method and reagent for measuring cholesterol in high-density lipoproteins |
| WO2004055204A1 (en) * | 2002-12-13 | 2004-07-01 | Denka Seiken Co., Ltd. | Method of multiple quantification of cholesterol in low-density lipoproteins |
| JP4643212B2 (en) * | 2003-11-28 | 2011-03-02 | シスメックス株式会社 | Cholesterol dehydrogenase stabilization method, cholesterol dehydrogenase-containing composition, and cholesterol measurement reagent |
| US7799537B2 (en) * | 2004-11-29 | 2010-09-21 | Otsuka Pharmaceutical Co., Ltd. | Cholesterol measuring reagent containing a cholesterol esterase |
| CN101120097B (en) * | 2005-02-14 | 2013-03-06 | 协和梅迪克斯株式会社 | Method, reagent and kit for quantifying cholesterol in remnant-like lipoprotein (RLP) |
| JP4735069B2 (en) * | 2005-06-16 | 2011-07-27 | 東ソー株式会社 | Judgment method of drug treatment effect by ultra-low density lipoprotein cholesterol level |
| KR102211991B1 (en) | 2011-11-11 | 2021-02-05 | 엑시스-시일드 에이에스 | Blood sample assay method |
| TWI507671B (en) * | 2012-04-20 | 2015-11-11 | Denka Seiken Kk | Method of erasing triglyceride in lipoproteins other than low density lipoprotein |
| CN105137098B (en) * | 2015-08-24 | 2017-01-25 | 巩晓东 | Determination reagent of cholesterols in serum high density lipoprotein |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2994831B2 (en) | 1991-12-27 | 1999-12-27 | 国際試薬株式会社 | Cholesterol assay and reagents |
| JP3107492B2 (en) | 1994-04-05 | 2000-11-06 | 国際試薬株式会社 | Method for quantification of components in lipoprotein fraction |
| WO1996029599A1 (en) | 1995-03-20 | 1996-09-26 | Kyowa Medex Co., Ltd. | Method of quantifying cholesterol in low-density or very-low-density lipoprotein |
| JP3614485B2 (en) | 1994-09-12 | 2005-01-26 | 国際試薬株式会社 | Method for quantifying cholesterol in lipoprotein fractions |
| JP3446486B2 (en) | 1995-07-21 | 2003-09-16 | 和光純薬工業株式会社 | Method for measuring components in lipoproteins |
| JPH0943234A (en) | 1995-07-31 | 1997-02-14 | Iatron Lab Inc | Measuring reagent and measuring method for preventing turbidity derived from heparin |
| JP3193634B2 (en) | 1996-05-29 | 2001-07-30 | 第一化学薬品株式会社 | LDL cholesterol determination method |
| JP4034851B2 (en) * | 1997-06-19 | 2008-01-16 | 株式会社三菱化学ヤトロン | Specific measurement method and measurement composition for low density lipoprotein cholesterol, and specific measurement method for low density and high density lipoprotein cholesterol |
| US6114134A (en) * | 1997-06-25 | 2000-09-05 | International Reagents Corporation | Method for assaying biological specimens for substances contained in the components thereof and reagent to be used in this method |
| DE69840725D1 (en) * | 1997-06-25 | 2009-05-20 | Sysmex Corp | METHOD AND REAGENTS FOR DETERMINING SUBSTANCES IN BIOLOGICAL SAMPLES |
| US6126090A (en) | 1999-01-12 | 2000-10-03 | Calmar Inc. | Nozzle cap for trigger sprayer |
| US6986998B2 (en) * | 2000-06-07 | 2006-01-17 | International Reagents Corporation | Method of analyzing components in biological samples |
-
2001
- 2001-06-05 US US10/297,241 patent/US6986998B2/en not_active Expired - Lifetime
- 2001-06-05 DE DE60140251T patent/DE60140251D1/en not_active Expired - Lifetime
- 2001-06-05 AT AT01934540T patent/ATE446378T1/en not_active IP Right Cessation
- 2001-06-05 EP EP01934540A patent/EP1288306B1/en not_active Expired - Lifetime
- 2001-06-05 AU AU2001260714A patent/AU2001260714A1/en not_active Abandoned
- 2001-06-05 WO PCT/JP2001/004721 patent/WO2001094619A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1288306B1 (en) | 2009-10-21 |
| DE60140251D1 (en) | 2009-12-03 |
| US20030129681A1 (en) | 2003-07-10 |
| EP1288306A4 (en) | 2006-05-10 |
| US6986998B2 (en) | 2006-01-17 |
| EP1288306A1 (en) | 2003-03-05 |
| ATE446378T1 (en) | 2009-11-15 |
| WO2001094619A1 (en) | 2001-12-13 |
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