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AU2001259672A1 - Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics - Google Patents

Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Info

Publication number
AU2001259672A1
AU2001259672A1 AU2001259672A AU5967201A AU2001259672A1 AU 2001259672 A1 AU2001259672 A1 AU 2001259672A1 AU 2001259672 A AU2001259672 A AU 2001259672A AU 5967201 A AU5967201 A AU 5967201A AU 2001259672 A1 AU2001259672 A1 AU 2001259672A1
Authority
AU
Australia
Prior art keywords
polynucleotides
identification
methods
desired characteristics
enzymatic assembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001259672A
Inventor
Simon Delagrave
Barry Marrs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hercules LLC
Original Assignee
Hercules LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hercules LLC filed Critical Hercules LLC
Publication of AU2001259672A1 publication Critical patent/AU2001259672A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001259672A 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics Abandoned AU2001259672A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09571774 2000-05-16
US09/571,774 US6479262B1 (en) 2000-05-16 2000-05-16 Solid phase enzymatic assembly of polynucleotides
PCT/US2001/014966 WO2001088173A2 (en) 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Publications (1)

Publication Number Publication Date
AU2001259672A1 true AU2001259672A1 (en) 2001-11-26

Family

ID=24284992

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001259672A Abandoned AU2001259672A1 (en) 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Country Status (5)

Country Link
US (2) US6479262B1 (en)
EP (1) EP1282698A2 (en)
AR (1) AR028454A1 (en)
AU (1) AU2001259672A1 (en)
WO (1) WO2001088173A2 (en)

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US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
JP2005530548A (en) * 2002-06-25 2005-10-13 ウルトラシェイプ インコーポレイティド Apparatus and method effective for body aesthetics
NL1022537C2 (en) * 2003-01-31 2004-08-03 Univ Delft Tech Production of chimeric polynucleotides useful for producing chimeric polypeptides comprises blunt-ending two polynucleotides and ligating the blunt ends
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US9068209B2 (en) 2008-05-14 2015-06-30 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
DK3211126T3 (en) 2009-02-13 2020-12-21 X Chem Inc METHODS FOR FORMING AND SCREENING DNA-CODED LIBRARIES
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP4039363A1 (en) * 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
LT3360963T (en) 2010-11-12 2020-02-25 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2665833B1 (en) 2011-01-17 2017-04-19 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN102876658A (en) 2011-07-12 2013-01-16 北京唯尚立德生物科技有限公司 Method for large-scale synthesis of long-chain nucleic acid molecules
LT3594340T (en) 2011-08-26 2021-10-25 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
MX360438B (en) * 2011-09-07 2018-11-01 X Chem Inc Methods for tagging dna-encoded libraries.
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (en) 2012-04-24 2020-07-31 Gen9股份有限公司 Method for sorting nucleic acids and multiplex preparations in vitro cloning
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
EP2872680B1 (en) 2012-07-13 2018-04-04 X-Chem, Inc. Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
ES2810300T3 (en) * 2014-07-03 2021-03-08 Rhodx Inc Labeling and evaluation of a target sequence
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
TW201940695A (en) 2018-01-12 2019-10-16 英商卡美納生物科學公司 Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
CN115109813A (en) * 2022-02-09 2022-09-27 康龙化成(宁波)科技发展有限公司 Synthesis method for realizing 5' -phosphorylation and oligonucleotide coupling by one-pot method and application

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US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
DE3328937T1 (en) * 1982-02-01 1984-10-18 Innovax Laboratories, Ltd., Beverly Hills, Calif. Process for the production of defined polyribonucleotides
JPS61152695A (en) 1984-12-26 1986-07-11 Nippon Shinyaku Co Ltd Synthesis of long-chain dna
DE3546806C2 (en) 1985-03-30 1991-03-28 Marc Genf/Geneve Ch Ballivet
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
EP0427745A4 (en) 1988-07-14 1992-11-25 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
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EP0406937A3 (en) 1989-06-30 1992-01-22 Eastman Kodak Company In vitro gene synthesis
JPH07507201A (en) 1992-03-10 1995-08-10 ザ　ガバメント　オブ　ザ　ユナイテッド　ステイツ　オブ　アメリカ，アズ　レプリゼンテッド　バイ　ザ　セクレタリー，デパートメント　オブ　ヘルス　アンド　ヒューマン　サービシーズ variable template reaction
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
DE4343591A1 (en) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Process for the evolutionary design and synthesis of functional polymers based on shape elements and shape codes
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
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US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
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AU753605B2 (en) 1998-06-22 2002-10-24 Larova Biochemie Gmbh Production of polymers from nucleotidic and/or non-nucleotidic elements using enzymatic methods
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US20010053849A1 (en) 1999-06-16 2001-12-20 Mary Jeanne Kreek Plural biological sample arrays, and preparation and uses thereof

Also Published As

Publication number Publication date
WO2001088173A3 (en) 2002-06-06
US6635453B2 (en) 2003-10-21
US20030082536A1 (en) 2003-05-01
AR028454A1 (en) 2003-05-07
US6479262B1 (en) 2002-11-12
EP1282698A2 (en) 2003-02-12
WO2001088173A2 (en) 2001-11-22

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