AU2001241723A1 - Methods for multi-stage solid phase amplification of nucleic acids - Google Patents
Methods for multi-stage solid phase amplification of nucleic acidsInfo
- Publication number
- AU2001241723A1 AU2001241723A1 AU2001241723A AU4172301A AU2001241723A1 AU 2001241723 A1 AU2001241723 A1 AU 2001241723A1 AU 2001241723 A AU2001241723 A AU 2001241723A AU 4172301 A AU4172301 A AU 4172301A AU 2001241723 A1 AU2001241723 A1 AU 2001241723A1
- Authority
- AU
- Australia
- Prior art keywords
- methods
- nucleic acids
- solid phase
- phase amplification
- stage solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000003321 amplification Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 239000007790 solid phase Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/107—Chemical cleaving agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/131—Modifications characterised by incorporating a restriction site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/155—Particles of a defined size, e.g. nanoparticles
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51330000A | 2000-02-25 | 2000-02-25 | |
| US09/513,300 | 2000-02-25 | ||
| PCT/US2001/005940 WO2001062982A2 (en) | 2000-02-25 | 2001-02-23 | Methods for multi-stage solid phase amplification of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001241723A1 true AU2001241723A1 (en) | 2001-09-03 |
Family
ID=24042679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001241723A Abandoned AU2001241723A1 (en) | 2000-02-25 | 2001-02-23 | Methods for multi-stage solid phase amplification of nucleic acids |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2001241723A1 (en) |
| WO (1) | WO2001062982A2 (en) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1444364A4 (en) * | 2001-10-27 | 2006-03-15 | Sigma Genosys Inc | MULTIPLE OLIGONUCLEOTIDE PRO GENE FOR APPLICATION TO GEN-ARRAYS |
| CN103397082B (en) | 2003-02-26 | 2017-05-31 | 考利达基因组股份有限公司 | The random array DNA analysis carried out by hybridization |
| ES2949821T3 (en) | 2004-01-07 | 2023-10-03 | Illumina Cambridge Ltd | Molecular arrays |
| GB0402895D0 (en) * | 2004-02-10 | 2004-03-17 | Solexa Ltd | Arrayed polynucleotides |
| US20090264299A1 (en) | 2006-02-24 | 2009-10-22 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| EP2620510B2 (en) | 2005-06-15 | 2020-02-19 | Complete Genomics Inc. | Single molecule arrays for genetic and chemical analysis |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090075343A1 (en) | 2006-11-09 | 2009-03-19 | Complete Genomics, Inc. | Selection of dna adaptor orientation by nicking |
| WO2009052214A2 (en) | 2007-10-15 | 2009-04-23 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| JP2009178159A (en) * | 2007-11-05 | 2009-08-13 | Hitachi Plant Technologies Ltd | Nucleic acid sequence detection method and nucleic acid sequence detection substrate |
| WO2009073629A2 (en) | 2007-11-29 | 2009-06-11 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US9347092B2 (en) * | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| JP5457222B2 (en) * | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | Miniaturized high-throughput nucleic acid analysis |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| ES2966040T3 (en) | 2010-10-08 | 2024-04-18 | Harvard College | High throughput barcoded single cells |
| US8778848B2 (en) | 2011-06-09 | 2014-07-15 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
| GB2495909A (en) * | 2011-10-19 | 2013-05-01 | Genome Analysis Ct | Arrays comprising immobilized primer pair spots for amplifying target nucleic acids |
| WO2013063382A2 (en) | 2011-10-28 | 2013-05-02 | Illumina, Inc. | Microarray fabrication system and method |
| AU2012336040B2 (en) | 2011-11-07 | 2015-12-10 | Illumina, Inc. | Integrated sequencing apparatuses and methods of use |
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| DK3017065T3 (en) | 2013-07-01 | 2018-11-26 | Illumina Inc | Catalyst-free Surface functionalization and polymer grafting |
| WO2015095291A1 (en) | 2013-12-19 | 2015-06-25 | Illumina, Inc. | Substrates comprising nano-patterning surfaces and methods of preparing thereof |
| JP6824290B2 (en) | 2016-05-18 | 2021-02-03 | イルミナ インコーポレイテッド | Self-assembled patterning with a patterned hydrophobic surface |
| ES2937782T3 (en) | 2016-10-03 | 2023-03-31 | Illumina Inc | Fluorescent detection of amines and hydrazines and methods of testing the same |
| CN118086479A (en) | 2017-08-01 | 2024-05-28 | 深圳华大智造科技有限公司 | Nucleic acid sequencing methods |
| EP3696275B1 (en) | 2017-10-11 | 2025-12-24 | MGI Tech Co., Ltd. | Method for improving loading and stability of nucleic acid on solid support |
| CN115698320A (en) | 2020-07-29 | 2023-02-03 | 深圳华大智造科技股份有限公司 | Method for loading nucleic acid molecules on solid support |
| CN118076747A (en) | 2021-10-11 | 2024-05-24 | 深圳华大智造科技股份有限公司 | Application of saponin compounds in nucleic acid sequencing |
| CN119790161A (en) | 2022-09-09 | 2025-04-08 | 深圳华大生命科学研究院 | Application of heteroaromatic compounds in nucleic acid detection |
| WO2024243873A1 (en) | 2023-05-31 | 2024-12-05 | 深圳华大智造科技股份有限公司 | Method for adjusting volume of nucleic acid nanospheres and use thereof |
| WO2024254367A1 (en) | 2023-06-09 | 2024-12-12 | Illumina, Inc | Method for polishing a substrate |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
| US5641658A (en) * | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| WO1998044151A1 (en) * | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid amplification |
| US6300070B1 (en) * | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
-
2001
- 2001-02-23 WO PCT/US2001/005940 patent/WO2001062982A2/en not_active Ceased
- 2001-02-23 AU AU2001241723A patent/AU2001241723A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001062982A2 (en) | 2001-08-30 |
| WO2001062982A3 (en) | 2002-09-19 |
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