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AU2000275763A1 - Expression of functional eukaryotic proteins - Google Patents

Expression of functional eukaryotic proteins

Info

Publication number
AU2000275763A1
AU2000275763A1 AU2000275763A AU7576300A AU2000275763A1 AU 2000275763 A1 AU2000275763 A1 AU 2000275763A1 AU 2000275763 A AU2000275763 A AU 2000275763A AU 7576300 A AU7576300 A AU 7576300A AU 2000275763 A1 AU2000275763 A1 AU 2000275763A1
Authority
AU
Australia
Prior art keywords
expression
eukaryotic proteins
functional eukaryotic
functional
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2000275763A
Inventor
Frances H Arnold
Zhanglin Lin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology
Original Assignee
California Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology filed Critical California Institute of Technology
Publication of AU2000275763A1 publication Critical patent/AU2000275763A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2000275763A 2000-03-27 2000-09-01 Expression of functional eukaryotic proteins Abandoned AU2000275763A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53859100A 2000-03-27 2000-03-27
US09538591 2000-03-27
PCT/US2000/024795 WO2001072999A1 (en) 2000-03-27 2000-09-01 Expression of functional eukaryotic proteins

Publications (1)

Publication Number Publication Date
AU2000275763A1 true AU2000275763A1 (en) 2001-10-08

Family

ID=24147538

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2000275763A Abandoned AU2000275763A1 (en) 2000-03-27 2000-09-01 Expression of functional eukaryotic proteins

Country Status (2)

Country Link
AU (1) AU2000275763A1 (en)
WO (1) WO2001072999A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008075325A2 (en) * 2006-12-19 2008-06-26 Dublin City University Heme peroxidase polypeptides
EP2584035A1 (en) * 2011-10-19 2013-04-24 Technische Universität Graz Horseradish peroxidase isoenzymes
US20140363837A1 (en) * 2011-10-19 2014-12-11 Technische Universitaet Graz (Geaz University of Technology Horseradish Peroxidase Isoenzymes
CN104334723B (en) * 2012-05-31 2018-05-22 明治制果药业株式会社 Use the manufacturing method of the peroxidase recombinant protein from Western horseradish of filamentous fungi
US10876098B2 (en) 2015-11-13 2020-12-29 Consejo Superior De Investigaciones Cientificas (Csic) Polynucleotide, host cell and a method to recombinantly produce the protein encoded by said polynucleotide having peroxygenative activity
EP4127151A1 (en) * 2020-03-24 2023-02-08 Technische Universität Wien Polypeptides with peroxidase activity
US12448639B2 (en) 2020-03-24 2025-10-21 Technische Universität Wien Methods for producing heme peroxidases
EP3885436A1 (en) * 2020-03-24 2021-09-29 Technische Universität Wien Polypeptides with peroxidase activity
MX2023010883A (en) * 2021-03-15 2023-09-27 Cargill Inc THERMOLABLE PIGMENTS FOR MEAT SUBSTITUTES DERIVED BY MUTATION OF THE CORAL PIGMENT EQUINOPORA FORSKALIANA.
WO2024107119A1 (en) * 2022-11-17 2024-05-23 Agency For Science, Technology And Research T3 vectors for recombinant protein production in mammalian cells
CN117660273A (en) * 2023-10-23 2024-03-08 武汉爱博泰克生物科技有限公司 Strain for high-yield high-activity HRP and rapid screening method and application thereof
WO2025212767A1 (en) * 2024-04-02 2025-10-09 Watchmaker Genomics, Inc. Gene variant libraries and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741691A (en) * 1996-01-23 1998-04-21 California Institute Of Technology Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6902918B1 (en) * 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
MXPA01000224A (en) * 1998-07-28 2002-10-17 California Inst Of Techn Expression of functional eukaryotic proteins.

Also Published As

Publication number Publication date
WO2001072999A1 (en) 2001-10-04

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