ATE66695T1 - Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten. - Google Patents
Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.Info
- Publication number
- ATE66695T1 ATE66695T1 AT86118191T AT86118191T ATE66695T1 AT E66695 T1 ATE66695 T1 AT E66695T1 AT 86118191 T AT86118191 T AT 86118191T AT 86118191 T AT86118191 T AT 86118191T AT E66695 T1 ATE66695 T1 AT E66695T1
- Authority
- AT
- Austria
- Prior art keywords
- probe
- sensitivity
- procedure
- increasing
- nucleic acid
- Prior art date
Links
- 230000035945 sensitivity Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- 238000007899 nucleic acid hybridization Methods 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 5
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 125000006853 reporter group Chemical group 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP86118191A EP0269764B1 (de) | 1986-12-01 | 1986-12-01 | Verfahren zur Erhöhung der Empfindlichkeit von Nukleinsäure-Hybridisierungstesten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE66695T1 true ATE66695T1 (de) | 1991-09-15 |
Family
ID=8195687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT86118191T ATE66695T1 (de) | 1986-12-01 | 1986-12-01 | Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten. |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0269764B1 (de) |
| AT (1) | ATE66695T1 (de) |
| DE (1) | DE3681176D1 (de) |
| ES (1) | ES2025556T3 (de) |
| GR (1) | GR3003056T3 (de) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| ATE168724T1 (de) * | 1988-09-29 | 1998-08-15 | Chiron Corp | Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde |
| US5695926A (en) * | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| FR2663040B1 (fr) * | 1990-06-11 | 1995-09-15 | Bio Merieux | Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich. |
| CA2255599C (en) | 1996-04-25 | 2006-09-05 | Bioarray Solutions, Llc | Light-controlled electrokinetic assembly of particles near surfaces |
| US7144119B2 (en) | 1996-04-25 | 2006-12-05 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US6387707B1 (en) | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
| US6958245B2 (en) | 1996-04-25 | 2005-10-25 | Bioarray Solutions Ltd. | Array cytometry |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
| US20030027126A1 (en) | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US6406845B1 (en) | 1997-05-05 | 2002-06-18 | Trustees Of Tuft College | Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample |
| WO1998053093A1 (en) | 1997-05-23 | 1998-11-26 | Bioarray Solutions Llc | Color-encoding and in-situ interrogation of matrix-coupled chemical compounds |
| US7115884B1 (en) | 1997-10-06 | 2006-10-03 | Trustees Of Tufts College | Self-encoding fiber optic sensor |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| US6241980B1 (en) | 1997-11-04 | 2001-06-05 | Becton, Dickinson And Company | Sample processing method using ion exchange resin |
| JP2002505846A (ja) * | 1997-11-06 | 2002-02-26 | モザイク テクノロジーズ | シグナル増幅およびプロセッシングのための多重逐次的ポリヌクレオチド置換反応 |
| US6210910B1 (en) | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
| CA2335951C (en) | 1998-06-24 | 2013-07-30 | Mark S. Chee | Decoding of array sensors with microspheres |
| AU6412799A (en) * | 1998-10-05 | 2000-04-26 | Mosaic Technologies | Reverse displacement assay for detection of nucleic acid sequences |
| US6429027B1 (en) | 1998-12-28 | 2002-08-06 | Illumina, Inc. | Composite arrays utilizing microspheres |
| US7510841B2 (en) | 1998-12-28 | 2009-03-31 | Illumina, Inc. | Methods of making and using composite arrays for the detection of a plurality of target analytes |
| US6846460B1 (en) | 1999-01-29 | 2005-01-25 | Illumina, Inc. | Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| EP2360270B1 (de) | 1999-05-20 | 2016-11-09 | Illumina, Inc. | Kombinatorische Entschlüsselung von Zufallsnukleinsäurearrays |
| US6544732B1 (en) | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| US8481268B2 (en) | 1999-05-21 | 2013-07-09 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US8080380B2 (en) | 1999-05-21 | 2011-12-20 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| JP2003505036A (ja) * | 1999-07-16 | 2003-02-12 | アクララ バイオサイエンシーズ， インコーポレイテッド | 核酸決定のための多重鎖置換 |
| DK1218545T3 (da) | 1999-08-18 | 2012-02-20 | Illumina Inc | Fremgangsmåder til fremstilling af oligonukleotidopløsninger |
| US6942968B1 (en) | 1999-08-30 | 2005-09-13 | Illumina, Inc. | Array compositions for improved signal detection |
| US7361488B2 (en) | 2000-02-07 | 2008-04-22 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| EP1990428B1 (de) | 2000-02-07 | 2010-12-22 | Illumina, Inc. | Nukleinsäuredetektionsverfahren mit universellem Priming |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| CA2401962A1 (en) | 2000-02-07 | 2001-08-09 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US6913884B2 (en) | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
| US8076063B2 (en) | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| EP1967595A3 (de) | 2000-02-16 | 2008-12-03 | Illumina, Inc. | Paralleles Genotypisieren von mehreren Patientenproben |
| US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| US7057704B2 (en) | 2000-09-17 | 2006-06-06 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US20030045005A1 (en) | 2000-10-17 | 2003-03-06 | Michael Seul | Light-controlled electrokinetic assembly of particles near surfaces |
| US7691571B2 (en) | 2001-01-31 | 2010-04-06 | Mayo Foundation For Medical Education And Research | Detection of bordetella |
| ES2344594T3 (es) | 2001-01-31 | 2010-09-01 | Mayo Foundation For Medical Education And Research | Deteccion del virus del herpes simplex. |
| US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
| JP4377689B2 (ja) | 2001-10-15 | 2009-12-02 | バイオアレイ ソリューションズ リミテッド | 同時尋問及び酵素仲介検出による多型遺伝子座の複合分析 |
| US7499806B2 (en) | 2002-02-14 | 2009-03-03 | Illumina, Inc. | Image processing in microsphere arrays |
| US7074598B2 (en) | 2002-09-25 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of vancomycin-resistant enterococcus spp. |
| US7365176B2 (en) | 2002-09-26 | 2008-04-29 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
| US7074599B2 (en) | 2002-09-27 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of mecA-containing Staphylococcus spp. |
| WO2004047007A1 (en) | 2002-11-15 | 2004-06-03 | Bioarray Solutions, Ltd. | Analysis, secure access to, and transmission of array images |
| EP1664722B1 (de) | 2003-09-22 | 2011-11-02 | Bioarray Solutions Ltd | Oberflächenimmobilisierter polyelektrolyt mit mehreren, zur kovalenten bindung an biomoleküle fähigen funktionellen gruppen |
| WO2005042763A2 (en) | 2003-10-28 | 2005-05-12 | Bioarray Solutions Ltd. | Optimization of gene expression analysis using immobilized capture probes |
| US7427475B2 (en) | 2003-11-18 | 2008-09-23 | Mayo Foundation For Medical Education And Research | Detection of group B streptococcus |
| US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
| US20060246576A1 (en) | 2005-04-06 | 2006-11-02 | Affymetrix, Inc. | Fluidic system and method for processing biological microarrays in personal instrumentation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| JPS6091999A (ja) * | 1983-10-25 | 1985-05-23 | Fujirebio Inc | ポリヌクレオチドの測定方法 |
| US4766064A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay employing polyether and diagnostic kit |
| US4849077A (en) * | 1984-08-06 | 1989-07-18 | Akademie Der Wissenschaften Der Ddr | Process for solid phase-sequencing of nucleic acid fragments |
-
1986
- 1986-12-01 AT AT86118191T patent/ATE66695T1/de not_active IP Right Cessation
- 1986-12-01 DE DE8686118191T patent/DE3681176D1/de not_active Expired - Lifetime
- 1986-12-01 EP EP86118191A patent/EP0269764B1/de not_active Expired - Lifetime
- 1986-12-01 ES ES198686118191T patent/ES2025556T3/es not_active Expired - Lifetime
-
1991
- 1991-11-04 GR GR91401657T patent/GR3003056T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0269764B1 (de) | 1991-08-28 |
| EP0269764A1 (de) | 1988-06-08 |
| ES2025556T3 (es) | 1992-04-01 |
| GR3003056T3 (en) | 1993-02-17 |
| DE3681176D1 (de) | 1991-10-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |