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ATE450623T1 - Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren - Google Patents

Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren

Info

Publication number
ATE450623T1
ATE450623T1 AT06013330T AT06013330T ATE450623T1 AT E450623 T1 ATE450623 T1 AT E450623T1 AT 06013330 T AT06013330 T AT 06013330T AT 06013330 T AT06013330 T AT 06013330T AT E450623 T1 ATE450623 T1 AT E450623T1
Authority
AT
Austria
Prior art keywords
analysis
nucleic acids
light emission
amplification
detection
Prior art date
Application number
AT06013330T
Other languages
English (en)
Inventor
Amar Gupta
Stephen Gordon Will
Original Assignee
Hoffmann La Roche
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Filing date
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Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE450623T1 publication Critical patent/ATE450623T1/de

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
    • C12Q1/707Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT06013330T 2005-06-30 2006-06-28 Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren ATE450623T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US69625305P 2005-06-30 2005-06-30
US69599105P 2005-06-30 2005-06-30
US69629305P 2005-06-30 2005-06-30
US69630305P 2005-06-30 2005-06-30

Publications (1)

Publication Number Publication Date
ATE450623T1 true ATE450623T1 (de) 2009-12-15

Family

ID=37104934

Family Applications (2)

Application Number Title Priority Date Filing Date
AT09168656T ATE507311T1 (de) 2005-06-30 2006-06-28 Lichtemissionsmodifikatoren und ihre verwendung bei der nukleinsäureerkennung, -verstärkung und - analyse
AT06013330T ATE450623T1 (de) 2005-06-30 2006-06-28 Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT09168656T ATE507311T1 (de) 2005-06-30 2006-06-28 Lichtemissionsmodifikatoren und ihre verwendung bei der nukleinsäureerkennung, -verstärkung und - analyse

Country Status (7)

Country Link
US (9) US20070072211A1 (de)
EP (5) EP1746172B1 (de)
JP (6) JP4634340B2 (de)
AT (2) ATE507311T1 (de)
CA (5) CA2895667C (de)
DE (1) DE602006010764D1 (de)
ES (3) ES2630186T3 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US20070072211A1 (en) 2005-06-30 2007-03-29 Roche Molecular Systems, Inc. Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
JP5593582B2 (ja) * 2007-06-12 2014-09-24 東洋紡株式会社 核酸の迅速な検出方法
JP2009036687A (ja) * 2007-08-03 2009-02-19 Panasonic Corp 遺伝子検出方法
US20100227314A1 (en) * 2007-08-09 2010-09-09 Dmitry Alexandrovich Gryadunov Method of identification of genotype and subtype of hepatitis c virus on a biological microchip
US20110189665A1 (en) * 2007-08-13 2011-08-04 Miller Jesse D Methods for detecting drug-resistant microbes
US8767952B2 (en) * 2007-12-17 2014-07-01 Broadcom Corporation Method and system for utilizing a single connection for efficient delivery of power and multimedia information
EP2336358A1 (de) * 2008-05-06 2011-06-22 QIAGEN GmbH Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion
EP2402462A1 (de) * 2008-06-06 2012-01-04 F. Hoffmann-La Roche AG Intern gesteuerte Multiplex-Erkennung und Quantifizierung von mikrobiellen Nukleinsäuren
US8658366B2 (en) * 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US8606527B2 (en) 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
WO2010120800A1 (en) * 2009-04-13 2010-10-21 Canon U.S. Life Sciences, Inc. A rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals
US9359639B2 (en) 2009-05-26 2016-06-07 Xiamen University Method for the detection of multiple single nucleotide variations or single nucleotide polymorphisms in a single tube
WO2010135917A1 (zh) 2009-05-26 2010-12-02 厦门大学 一种检测核酸序列变异的方法
US8148515B1 (en) 2009-06-02 2012-04-03 Biotium, Inc. Detection using a dye and a dye modifier
JP2011062088A (ja) * 2009-09-15 2011-03-31 Ihi Corp レジオネラ菌検出方法
US12410463B2 (en) * 2009-09-18 2025-09-09 The Trustees Of The University Of Pennsylvania Molecular beacons
US9677125B2 (en) * 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
BR112012009108A2 (pt) * 2009-10-21 2019-09-17 Koninl Philips Electronics Nv método para obtenção de informação de uma curva de amplificação de uma sequência de ácido núcleico alvo, mídia legível por máquina e equipamento para a análise de amostras de ácido nucleíco
JP5623888B2 (ja) * 2009-12-10 2014-11-12 エフ.ホフマン−ラ ロシュアーゲーF.Hoffmann−La Roche Aktiengesellschaft 分析対象物を分離して検出する方法
EP2556458B1 (de) 2010-04-09 2020-06-03 Life Technologies Corporation Visualisierungswerkzeug für qpcr-genotypisierungs-daten
WO2011160887A1 (en) * 2010-06-21 2011-12-29 Siemens Healthcare Diagnostics Inc. Soluble quencher to reduce background in qpcr assays
US9175332B2 (en) * 2010-07-29 2015-11-03 Roche Molecular Systems, Inc. Generic PCR
EP2614157A4 (de) * 2010-09-08 2014-03-26 Univ Brandeis Zusammensetzungen und verfahren für auf nukleinsäuren basierende diagnoseassays für ziele mit variabler sequenz
JP5722001B2 (ja) * 2010-11-10 2015-05-20 株式会社日立ハイテクノロジーズ 遺伝子検査方法及び検査装置
JP5985503B2 (ja) 2010-12-16 2016-09-06 バイオ−ラッド ラボラトリーズ インコーポレーティッド 定量的増幅のためのユニバーサルリファレンス色素
US20120164641A1 (en) * 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
JP6013328B2 (ja) 2011-04-18 2016-10-25 オリンパス株式会社 標的粒子の定量方法
CN103608663B (zh) * 2011-04-20 2016-01-13 奥林巴斯株式会社 生物样品中的核酸分子的检测方法
WO2013002261A1 (ja) * 2011-06-27 2013-01-03 オリンパス株式会社 標的粒子の検出方法
WO2013040060A2 (en) * 2011-09-12 2013-03-21 Pathogenica, Inc. Nucleic acids for multiplex detection of hepatitis c virus
EP4361608A3 (de) 2012-02-03 2024-07-24 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
CN102766701B (zh) * 2012-07-04 2016-05-04 福州泰普生物科学有限公司 丙型肝炎病毒基因分型的试剂盒及方法
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP3901243A1 (de) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
EP2722398B1 (de) * 2012-10-18 2017-07-12 F. Hoffmann-La Roche AG Test mit zwei Sonden zur Erkennung von HCV
ES2659747T3 (es) 2012-10-18 2018-03-19 F. Hoffmann-La Roche Ag Ensayo de doble sonda para la detección de poblaciones heterogéneas de amplicón
CN105143847B (zh) * 2013-02-05 2019-05-28 三路影像公司 细胞染色组合物及其用途
CA2960776C (en) 2013-09-13 2021-11-16 Rutgers, The State University Of New Jersey Multiplex diagnostic assay for lyme disease and other tick-borne diseases
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
EP3426805B1 (de) * 2016-03-11 2023-11-29 Roche Diagnostics GmbH Zusammensetzungen und verfahren zum nachweis des zikavirus
WO2017218777A1 (en) * 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
US11447835B2 (en) * 2016-10-19 2022-09-20 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis C virus
CN108546748A (zh) * 2017-04-05 2018-09-18 杭州丹威生物科技有限公司 一种检测核酸的方法和试剂盒
WO2019070854A1 (en) 2017-10-03 2019-04-11 Abbott Molecular Inc. ASSAY FOR DETECTION OF HEPATITIS C VIRUS (HCV)
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
KR102063864B1 (ko) * 2018-05-16 2020-01-08 사회복지법인 삼성생명공익재단 표면-증강 라만 산란 기반의 감염 질환 진단용 마커 검출 방법
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
CN112766296B (zh) * 2019-11-06 2023-04-07 济南信通达电气科技有限公司 输电线路安全隐患目标检测模型训练方法及装置
WO2021236116A1 (en) * 2020-05-20 2021-11-25 Delahoussaye Kevin Antiviral medicinal template
JP2024500169A (ja) 2020-12-22 2024-01-04 エフ. ホフマン-ラ ロシュ アーゲー 大ストークスシフト蛍光色素を使用して多重化リアルタイムpcrを行うための方法
KR102533568B1 (ko) * 2020-12-23 2023-05-16 건국대학교 산학협력단 라이트 업 RNA 앱타머를 사용한 형광 기반 다중 miRNA 검출 시스템
KR102465706B1 (ko) * 2020-12-23 2022-11-09 건국대학교 산학협력단 Dna 주형 구리 나노입자 기반 fret 센서 및 이를 이용한 표적 핵산 분자의 검출 방법
CN114540526B (zh) * 2022-03-23 2023-10-31 福建医科大学孟超肝胆医院(福州市传染病医院) 对五种输入性疟原虫分型检测的引物、探针及方法

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US6251581B1 (en) * 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
ATE161291T1 (de) * 1991-08-27 1998-01-15 Hoffmann La Roche Verfahren und reagenzien zum nachweis von hepatitis c
DE637965T1 (de) 1991-11-26 1995-12-14 Gilead Sciences Inc Gesteigerte bildung von triple- und doppelhelices aus oligomeren mit modifizierten pyrimidinen.
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5484908A (en) 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
CA2128528C (en) * 1992-11-27 2012-02-14 Geert Maertens Process for typing of hcv isolates
EP0984068B1 (de) 1993-04-27 2012-03-28 Innogenetics N.V. Sequenzen von Hepatitis C Virus-Genotypen sowie ihre Verwendungen als Therapeutika und Diagnostika
US5882852A (en) * 1993-06-29 1999-03-16 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Hepatitic C virus (HCV) core gene nucleotide sequences and related methods of detecting major and minor genotypes of HCV isolates
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
ES2176342T5 (es) 1994-10-21 2010-02-09 Innogenetics N.V. Secuencias del genotipo 7 del virus de la hepatitis c y su utilizacion como agentes profilacticos, terapeuticos y de diagnosticos.
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5795729A (en) * 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
ATE280177T1 (de) 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
JPH11148521A (ja) 1997-11-13 1999-06-02 Nichias Corp 鳴き防止シム
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
AU3274600A (en) 1999-03-18 2000-10-09 Exiqon A/S One step sample preparation and detection of nucleic acids in complex biologicalsamples
IL145496A0 (en) 1999-03-24 2002-06-30 Exiqon As Improved synthesis of [2.2.1] bicyclo nucleosides
US6734291B2 (en) 1999-03-24 2004-05-11 Exiqon A/S Synthesis of [2.2.1]bicyclo nucleosides
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
DE69915468T2 (de) 1999-09-01 2005-01-20 Invitrogen Corp., Carlsbad Photon Dämpfer für Fluoreszenzassays
WO2001046469A1 (fr) 1999-12-22 2001-06-28 Otsuka Pharmaceutical Co., Ltd. Procede d'etude du genotype du virus de l'hepatite c
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
KR100489326B1 (ko) 1999-12-27 2005-05-16 아사히 가세이 가부시키가이샤 글리신의 제조 방법
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
JP2002345467A (ja) 2000-10-23 2002-12-03 Srl Inc C型肝炎ウイルスの型分類用プローブ及びそれを用いたc型肝炎ウイルスの型分類方法並びにそれを用いたインターフェロン投与の治療効果を予測する方法。
KR20040034582A (ko) 2000-12-04 2004-04-28 프리마겐 홀딩 비.브이. 내공생체 세포소기관의 시험 및 그것에 의해 동정할 수있는 화합물
CN1366067A (zh) * 2001-01-15 2002-08-28 上海博华基因芯片技术有限公司 一种乙型、丙型肝炎双检基因芯片试剂盒及其制备和使用方法
EP1236804A1 (de) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
JP2002272475A (ja) 2001-03-22 2002-09-24 Eiken Chem Co Ltd 蛍光偏光法による核酸増幅産物の検出方法
US7179600B2 (en) 2001-04-12 2007-02-20 Biocore Co., Ltd. Oligonucleotide chip composition and a method for analyzing a hepatitis C virus genotype using the composition
JP2003084002A (ja) * 2001-06-27 2003-03-19 Fuji Photo Film Co Ltd 蛍光検出におけるバックグラウンドの低減方法
US6800765B2 (en) * 2001-08-02 2004-10-05 Molecular Devices Corporation Fluorescent pH indicators for intracellular assays
US7196183B2 (en) * 2001-08-31 2007-03-27 Innogenetics N.V. Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent
JPWO2003040367A1 (ja) 2001-11-08 2005-03-03 三光純薬株式会社 遺伝子増幅反応で合成されたオリゴヌクレオチドによる自己集合体の形成方法、自己集合体及び遺伝子の検出方法
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
US20030136921A1 (en) * 2002-01-23 2003-07-24 Reel Richard T Methods for fluorescence detection that minimizes undesirable background fluorescence
JP4312711B2 (ja) * 2002-05-20 2009-08-12 ブリストル−マイヤーズ スクイブ カンパニー ヘテロ環式スルホンアミドc型肝炎ウイルス阻害剤
US20040005613A1 (en) * 2002-05-22 2004-01-08 Norton Michael L. Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms
US7195885B2 (en) * 2002-08-12 2007-03-27 Bristol-Myers Squibb Company Hepatitis C virus assays
US7183302B2 (en) 2002-08-12 2007-02-27 Bristol-Myers Squibb Company Iminothiazolidinones as inhibitors of HCV replication
GB0223563D0 (en) 2002-10-10 2002-11-20 Secr Defence Detection system
WO2004044182A2 (en) 2002-11-13 2004-05-27 Washington University Highly permissive cell lines for hepatitis c virus replication
CA2506797C (en) 2002-11-22 2009-02-10 F.Hoffmann-La Roche Ag Detectable labeled nucleoside analogs and methods of use thereof
EP1431398A1 (de) * 2002-12-20 2004-06-23 Evotec OAI AG Verfahren zum Nachweis die Menge eines Analyten in einer Mischung
US20040229253A1 (en) * 2003-02-18 2004-11-18 Hyldig-Nielsen Jens J. Compositions and methods for multiplex analysis of polynucleotides
JP2007505606A (ja) 2003-09-16 2007-03-15 サーナ・セラピューティクス・インコーポレイテッド 低分子干渉核酸(siNA)を使用したC型肝炎ウィルス(HCV)発現のRNA干渉媒介性抑制
US20070072211A1 (en) 2005-06-30 2007-03-29 Roche Molecular Systems, Inc. Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids

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JP6026977B2 (ja) 2016-11-16
US8067208B2 (en) 2011-11-29
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