[go: up one dir, main page]

ATE333499T1 - Selektion/subtraktionsansatz zur genidentifizierung - Google Patents

Selektion/subtraktionsansatz zur genidentifizierung

Info

Publication number
ATE333499T1
ATE333499T1 AT99912674T AT99912674T ATE333499T1 AT E333499 T1 ATE333499 T1 AT E333499T1 AT 99912674 T AT99912674 T AT 99912674T AT 99912674 T AT99912674 T AT 99912674T AT E333499 T1 ATE333499 T1 AT E333499T1
Authority
AT
Austria
Prior art keywords
selection
expression library
clone
clones
cell
Prior art date
Application number
AT99912674T
Other languages
English (en)
Inventor
Andrei Gudkov
Roman Kondratov
Original Assignee
Quark Biotech Inc
Univ Illinois
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quark Biotech Inc, Univ Illinois filed Critical Quark Biotech Inc
Application granted granted Critical
Publication of ATE333499T1 publication Critical patent/ATE333499T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT99912674T 1998-03-18 1999-03-18 Selektion/subtraktionsansatz zur genidentifizierung ATE333499T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7843298P 1998-03-18 1998-03-18

Publications (1)

Publication Number Publication Date
ATE333499T1 true ATE333499T1 (de) 2006-08-15

Family

ID=22143995

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99912674T ATE333499T1 (de) 1998-03-18 1999-03-18 Selektion/subtraktionsansatz zur genidentifizierung

Country Status (9)

Country Link
EP (1) EP1066369B1 (de)
JP (1) JP2002506635A (de)
AT (1) ATE333499T1 (de)
AU (1) AU758565B2 (de)
CA (1) CA2323633A1 (de)
DE (1) DE69932418D1 (de)
IL (1) IL138287A0 (de)
WO (1) WO1999047643A1 (de)
ZA (1) ZA200004711B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU774332B2 (en) 1999-03-12 2004-06-24 Gpc Biotech Inc. Methods and reagents for identifying synthetic genetic elements
WO2001007598A1 (en) * 1999-07-22 2001-02-01 Children's Medical Center Corporation EXPRESSION CLONING USING A TAGGED cDNA LIBRARY
US6582899B1 (en) * 2000-02-15 2003-06-24 Deltagen Proteomics, Inc. Methods for identifying agents that cause a lethal phenotype, and agents thereof
AU2001287273A1 (en) * 2000-03-24 2001-10-08 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
JP2003528605A (ja) 2000-03-28 2003-09-30 ユニヴァーシティー オヴ ロチェスター ライブラリーを作成する方法および目的のポリヌクレオチドを選択する方法
WO2002010438A2 (en) * 2000-07-28 2002-02-07 The Johns Hopkins University Serial analysis of transcript expression using long tags
US6455255B1 (en) * 2000-08-02 2002-09-24 Abbott Laboratories Method of performing subtractive hybridization using RDA
US6696441B1 (en) 2000-08-11 2004-02-24 The Regents Of The University Of California Inhibition of p53-induced stress response
US7576692B2 (en) 2001-02-23 2009-08-18 Nokia Corporation Method for positioning, a positioning system, and an electronic device
US7601846B2 (en) 2001-06-26 2009-10-13 The Regents Of The University Of California Compounds having activity as inhibitors of apoptosis
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
WO2004007684A2 (en) * 2002-07-12 2004-01-22 Affymetrix, Inc. Synthetic tag genes
CA2594832A1 (en) * 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
EP1963531B1 (de) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporter und verfahren zu deren herstellung und verwendung
WO2008054543A2 (en) * 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
ES2178101T3 (es) * 1994-12-09 2002-12-16 Imp College Innovations Ltd Genes de virulencia en la region vgc2 de salmonella.

Also Published As

Publication number Publication date
EP1066369A1 (de) 2001-01-10
DE69932418D1 (de) 2006-08-31
AU3099699A (en) 1999-10-11
IL138287A0 (en) 2001-10-31
ZA200004711B (en) 2001-10-31
WO1999047643A9 (en) 1999-11-18
EP1066369B1 (de) 2006-07-19
AU758565B2 (en) 2003-03-27
WO1999047643A1 (en) 1999-09-23
CA2323633A1 (en) 1999-09-23
JP2002506635A (ja) 2002-03-05
EP1066369A4 (de) 2005-03-02

Similar Documents

Publication Publication Date Title
ATE333499T1 (de) Selektion/subtraktionsansatz zur genidentifizierung
Zhang et al. A single-cell analysis of the Arabidopsis vegetative shoot apex
Huang et al. A pericycle‐localized silicon transporter for efficient xylem loading in rice
Park et al. RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis
Gray et al. Control of translation initiation in animals
Thomas et al. Identification of an intronic splicing regulatory element involved in auto‐regulation of alternative splicing of SCL33 pre‐mRNA
Lee et al. Deep functional redundancy between FAMA and FOUR LIPS in stomatal development
Bryson‐Richardson et al. Myosin heavy chain expression in zebrafish and slow muscle composition
Saeed et al. Moving beyond DNA sequence to improve plant stress responses
Smit et al. Specification and regulation of vascular tissue identity in the Arabidopsis embryo
KR930702521A (ko) 효율적인 유전적 억제요소를 얻는 방법 및 그 용도
Lara Pinto et al. Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil
Langlais et al. Polycomb group proteins bind an engrailed PRE in both the “ON” and “OFF” transcriptional states of engrailed
Sharakhov et al. Heterochromatin, histone modifications, and nuclear architecture in disease vectors
Sarkies et al. Propagation of histone marks and epigenetic memory during normal and interrupted DNA replication
Rezende et al. Head transcriptomes of two closely related species of fruit flies of the Anastrepha fraterculus group reveals divergent genes in species with extensive gene flow
Yu et al. Genome of elegance coral Catalaphyllia jardinei (Euphylliidae)
Jockusch et al. Something old, something new, something borrowed, something red: the origin of ecologically relevant novelties in Hemiptera
Calarco et al. Detecting gene expression in Caenorhabditis elegans
DE60115225D1 (de) Verfahren zur bestimmung einer genfunktion
Chu et al. Development and use of a piggyBac‐based jumpstarter system in Drosophila suzukii
Igarashi et al. Cloning and characterization of the Xenopus laevis p8 gene
Tremblay et al. Mechanisms of epigenetic regulation of transcription by lncRNAs in plants
Shin et al. Chromatin regulation during C. elegans germline development
Kim et al. Tagged library approach facilitates forward chemical genetics

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties