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ATE321867T1 - Zusammensetzungen und methoden zur verwendung von dna polymerasen - Google Patents

Zusammensetzungen und methoden zur verwendung von dna polymerasen

Info

Publication number
ATE321867T1
ATE321867T1 AT00973960T AT00973960T ATE321867T1 AT E321867 T1 ATE321867 T1 AT E321867T1 AT 00973960 T AT00973960 T AT 00973960T AT 00973960 T AT00973960 T AT 00973960T AT E321867 T1 ATE321867 T1 AT E321867T1
Authority
AT
Austria
Prior art keywords
compositions
methods
polymerase
dna polymerases
mutant
Prior art date
Application number
AT00973960T
Other languages
English (en)
Inventor
Joseph A Sorge
Holly H Hogrefe
Connie J Hansen
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of ATE321867T1 publication Critical patent/ATE321867T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mathematical Physics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00973960T 1999-10-29 2000-10-27 Zusammensetzungen und methoden zur verwendung von dna polymerasen ATE321867T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16260099P 1999-10-29 1999-10-29

Publications (1)

Publication Number Publication Date
ATE321867T1 true ATE321867T1 (de) 2006-04-15

Family

ID=22586340

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00973960T ATE321867T1 (de) 1999-10-29 2000-10-27 Zusammensetzungen und methoden zur verwendung von dna polymerasen

Country Status (7)

Country Link
US (2) US6946273B1 (de)
EP (1) EP1226255B1 (de)
JP (1) JP4949585B2 (de)
AT (1) ATE321867T1 (de)
AU (1) AU1239901A (de)
DE (1) DE60027040T2 (de)
WO (1) WO2001032887A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US6803201B2 (en) 2002-01-24 2004-10-12 Stratagene Compositions and methods for polynucleotide sequence determination
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
JP2006507012A (ja) * 2002-10-25 2006-03-02 ストラタジーン カリフォルニア 減少した塩基類似体検出活性を有するｄｎａポリメラーゼ
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
EP2292767B1 (de) 2004-06-04 2013-11-20 Takara Bio, Inc. Polypeptide mit DNA-Polymeraseaktivität
EP1888743B1 (de) 2005-05-10 2011-08-03 Illumina Cambridge Limited Verbesserte polymerasen
KR100757280B1 (ko) 2005-11-01 2007-09-11 한국해양연구원 고호열성 신균주 ｋｃｔｃ 10859ｂｐ 및 이로부터 생산되는고호열성 아밀라아제
EP1914303A1 (de) * 2006-10-09 2008-04-23 Qiagen GmbH Thermus eggertssonii DNA Polymerasen
KR100777230B1 (ko) * 2006-11-30 2007-11-28 한국해양연구원 써모코커스 유래 돌연변이 ｄｎａ 중합효소들 및 그의유전자들
EP2097523B1 (de) * 2006-12-29 2014-10-29 Agilent Technologies, Inc. Zusammensetzungen und verfahren unter verwendung von gespaltenen polymerasen
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
WO2009135093A2 (en) 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2352818B1 (de) 2008-11-03 2016-02-10 Kapa Biosystems, Inc. Chimäre dna-polymerasen
WO2010068680A1 (en) 2008-12-10 2010-06-17 Mount Sinai School Of Medicine Of New York University Thelper cell type 17 lineage-specific adjuvants, compositions and methods
EP2478373B1 (de) 2009-09-14 2019-01-09 Icahn School of Medicine at Mount Sinai Verfahren zur charakterisierung der antikörperbindungsaffinität und der epitopdiversität bei lebensmittelallergien
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
WO2012154934A1 (en) 2011-05-11 2012-11-15 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2013209499B2 (en) 2012-01-20 2018-05-10 Sequenom, Inc. Diagnostic processes that factor experimental conditions
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102264617B1 (ko) 2012-06-14 2021-06-14 라이프 테크놀로지스 코포레이션 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6478446B2 (ja) 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
LT2981921T (lt) 2013-04-03 2023-02-27 Sequenom, Inc. Neinvazinio genetinių variacijų vertinimo būdai ir procesai
WO2014190286A2 (en) 2013-05-24 2014-11-27 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102784584B1 (ko) 2013-06-21 2025-03-19 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
HRP20200250T1 (hr) 2013-08-08 2020-05-29 The Scripps Research Institute Metoda site-specifično enzimsko obilježavanje nukleinskih kiselina in vitro ugradnjom neprirodnih nukleotida
US10964409B2 (en) 2013-10-04 2021-03-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN111863131A (zh) 2013-10-07 2020-10-30 塞昆纳姆股份有限公司 用于非侵入性评估染色体改变的方法和过程
AU2014348323B2 (en) 2013-11-14 2020-09-24 Integrated Dna Technologies, Inc. DNA polymerase mutants having enhanced template discrimination activity
US10513706B2 (en) 2014-04-09 2019-12-24 The Scripps Research Institute Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters
EP3175000B1 (de) 2014-07-30 2020-07-29 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
EP3218471A1 (de) 2014-11-14 2017-09-20 Illumina, Inc. Polymerasen
EP3347461A1 (de) * 2015-09-09 2018-07-18 Qiagen GmbH Polymeraseenzym
WO2017106767A1 (en) 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
JP6959931B2 (ja) * 2016-02-29 2021-11-05 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ
US20190176153A1 (en) 2016-05-18 2019-06-13 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
EP3464629B1 (de) 2016-06-01 2021-09-08 F. Hoffmann-La Roche AG Immuno-pete
ES2929047T3 (es) 2016-06-24 2022-11-24 Scripps Research Inst Transportador de nucleósido trifosfato novedoso y usos del mismo
US10907204B2 (en) 2016-07-12 2021-02-02 Roche Sequencing Solutions, Inc. Primer extension target enrichment
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
IL319365A (en) 2017-01-24 2025-05-01 Sequenom Inc Methods and processes for assessing genetic variations
EP3580351A1 (de) * 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
JP6970205B2 (ja) 2017-03-08 2021-11-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Ｄｎａおよびｒｎａの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
PT3596233T (pt) 2017-03-17 2022-08-22 Sequenom Inc Métodos e processos para avaliação de mosaicismo genético
CN110573616B (zh) * 2017-04-18 2023-09-05 深圳华大智造科技股份有限公司 Phi29 DNA聚合酶及其编码基因与应用
EP3645710A1 (de) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophile dna-polymerase-mutanten
MA49716A (fr) 2017-07-11 2021-04-07 Scripps Research Inst Incorporation de nucléotides non naturels et procédés d'utilisationin vivo
CN111051512A (zh) 2017-07-11 2020-04-21 辛索克斯公司 非天然核苷酸的掺入及其方法
SG11202000939PA (en) 2017-08-03 2020-02-27 Synthorx Inc Cytokine conjugates for the treatment of proliferative and infectious diseases
IL319501A (en) 2017-12-29 2025-05-01 Scripps Research Inst Unnatural nucleotide compounds and methods of using them
AU2019374098B2 (en) 2018-10-31 2025-10-16 Illumina Singapore Pte. Ltd. Polymerases, compositions, and methods of use
KR20210098844A (ko) 2018-12-05 2021-08-11 일루미나, 인코포레이티드 중합효소, 조성물, 및 사용 방법
AU2020218203A1 (en) 2019-02-06 2021-08-26 Synthorx, Inc. IL-2 conjugates and methods of use thereof
EP3983543A4 (de) 2019-06-14 2023-05-03 The Scripps Research Institute Reagenzien und verfahren zur replikation, transkription und translation in halbsynthetischen organismen
CN114269917A (zh) 2019-08-19 2022-04-01 豪夫迈·罗氏有限公司 用于测序的dna和rna的单管制备
WO2021053008A1 (en) 2019-09-20 2021-03-25 F. Hoffmann-La Roche Ag Immune repertoire profiling by primer extension target enrichment
WO2021092431A1 (en) * 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
CN115956128A (zh) 2020-07-08 2023-04-11 豪夫迈·罗氏有限公司 在下一代测序文库的靶捕获过程中使用毒性引物对非靶文库分子进行靶向消耗
US12378596B2 (en) 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CA3206360A1 (en) 2020-12-24 2022-06-30 Integrated Dna Technologies, Inc. Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases
CN112779238B (zh) * 2020-12-28 2024-01-30 广州达安基因股份有限公司 用于丙肝病毒检测的dna聚合酶混合物
WO2022174898A1 (en) 2021-02-18 2022-08-25 F. Hoffmann-La Roche Ag Structure to prevent threading of nucleic acid templates through a nanopore during sequencing
WO2022197589A1 (en) 2021-03-15 2022-09-22 President And Fellows Of Harvard College Methods for in situ sequencing
EP4308723B1 (de) 2021-03-15 2025-04-23 F. Hoffmann-La Roche AG Gezielte sequenzierung der nächsten generation über verankerte primerextension
WO2022207682A1 (en) 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN114015672B (zh) * 2021-12-06 2022-05-31 江南大学 一种Pfu DNA聚合酶
US20250368983A1 (en) 2022-06-30 2025-12-04 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
WO2024244010A1 (zh) * 2023-06-02 2024-12-05 武汉华大智造科技有限公司 用于测序的dna聚合酶突变体
WO2024256358A2 (en) 2023-06-14 2024-12-19 F. Hoffmann-La Roche Ag Accelerated marking of 5-formyl cytosine and use in nucleic acid methylation sequencing
WO2025024676A1 (en) 2023-07-26 2025-01-30 Caribou Biosciences, Inc. In vitro validation methods for cd19-targeting cell therapies
WO2025193609A1 (en) 2024-03-11 2025-09-18 Caribou Biosciences, Inc. Methods and compositions for cost-effective assessment of nucleic acid transcripts and isoforms in cells
WO2025207517A2 (en) 2024-03-25 2025-10-02 Synthorx, Inc. Synthetic trna synthetases and cells comprising synthetic molecules for production of polypeptides
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
DE655506T1 (de) * 1994-10-17 1995-09-28 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
WO1996038568A1 (en) * 1995-05-31 1996-12-05 Amersham Life Science, Inc. Thermostable dna polymerases
AU2804597A (en) * 1996-04-15 1997-11-07 University Of Alberta Synthesis of fluorophore-labeled dna
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants

Also Published As

Publication number Publication date
DE60027040T2 (de) 2006-11-23
US20050158730A1 (en) 2005-07-21
EP1226255B1 (de) 2006-03-29
WO2001032887A1 (en) 2001-05-10
AU1239901A (en) 2001-05-14
JP4949585B2 (ja) 2012-06-13
EP1226255A1 (de) 2002-07-31
DE60027040D1 (de) 2006-05-18
US6946273B1 (en) 2005-09-20
JP2004500052A (ja) 2004-01-08

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