ATE319859T1 - Quantitative multiplex-pcr mit grossem dynamikbereich - Google Patents
Quantitative multiplex-pcr mit grossem dynamikbereichInfo
- Publication number
- ATE319859T1 ATE319859T1 AT02025794T AT02025794T ATE319859T1 AT E319859 T1 ATE319859 T1 AT E319859T1 AT 02025794 T AT02025794 T AT 02025794T AT 02025794 T AT02025794 T AT 02025794T AT E319859 T1 ATE319859 T1 AT E319859T1
- Authority
- AT
- Austria
- Prior art keywords
- dynamic range
- multiplex pcr
- large dynamic
- quantitative multiplex
- quantitative
- Prior art date
Links
- 238000007403 mPCR Methods 0.000 title abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
- G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33179101P | 2001-11-20 | 2001-11-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE319859T1 true ATE319859T1 (de) | 2006-03-15 |
Family
ID=23295393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02025794T ATE319859T1 (de) | 2001-11-20 | 2002-11-16 | Quantitative multiplex-pcr mit grossem dynamikbereich |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7118867B2 (de) |
| EP (1) | EP1312684B1 (de) |
| JP (1) | JP3909010B2 (de) |
| AT (1) | ATE319859T1 (de) |
| CA (1) | CA2407744C (de) |
| DE (1) | DE60209617T2 (de) |
| ES (1) | ES2260378T3 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070298423A1 (en) * | 2000-03-24 | 2007-12-27 | Eppendorf Array Technologies Sa (Eat) | Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays |
| US20080085515A1 (en) * | 2000-03-24 | 2008-04-10 | Eppendorf Array Technologies Sa (Eat) | Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays |
| US20060216737A1 (en) * | 2005-03-10 | 2006-09-28 | John Bodeau | Methods for multiplex amplification |
| US7875274B2 (en) * | 2005-12-16 | 2011-01-25 | The Board Of Trustees Of The Leland Stanford Junior University | Protein modulators of resistance to alkylating agents |
| JP4968577B2 (ja) * | 2006-04-11 | 2012-07-04 | エフ.ホフマン−ラ ロシュ アーゲー | サイトケラチン19(CK19)mRNAの迅速測定法、並びにそのためのプライマー及びプローブ |
| EP2220227B1 (de) * | 2007-07-13 | 2014-09-10 | The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense | Einmalige kalibrator-polynukleotide und verfahren zur verwendung bei quantitativen nukleinsäure-tests |
| US20090181391A1 (en) * | 2007-11-01 | 2009-07-16 | Zymo Research Corporation | Methods for analysis of dna methylation percentage |
| EP2245197B1 (de) * | 2008-02-07 | 2016-10-12 | Whitespace Enterprise Corporation | Verbesserungen bei und in verbindung mit der analyse |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| WO2010091400A2 (en) * | 2009-02-09 | 2010-08-12 | Frederic Zenhausern | Improvements in and relating to analysis |
| WO2010123626A1 (en) | 2009-04-24 | 2010-10-28 | University Of Southern California | Cd133 polymorphisms and expression predict clinical outcome in patients with cancer |
| WO2011084757A1 (en) | 2009-12-21 | 2011-07-14 | University Of Southern California | Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer |
| WO2011085334A1 (en) | 2010-01-11 | 2011-07-14 | University Of Southern California | Cd44 polymorphisms predict clinical outcome in patients with gastric cancer |
| CN103249844B (zh) | 2010-10-14 | 2015-06-24 | 瑞昂尼克公司 | 目标核酸多标靶定量鉴定 |
| EP2702170B1 (de) | 2011-04-28 | 2015-12-16 | Life Technologies Corporation | Verfahren und zusammensetzungen für multiplex-pcr |
| US20130059762A1 (en) | 2011-04-28 | 2013-03-07 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| US20130059738A1 (en) | 2011-04-28 | 2013-03-07 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| CN107119137B (zh) * | 2017-05-26 | 2018-04-06 | 广州华弘生物科技有限公司 | 一种快速检测HER‑2/neu基因表达的检测试剂盒 |
| JP2021153393A (ja) * | 2020-03-25 | 2021-10-07 | 東洋紡株式会社 | 核酸増幅反応用組成物 |
| CN113462761B (zh) * | 2021-07-30 | 2024-02-09 | 宁波胤瑞生物医学仪器有限责任公司 | 一种检测her2基因的引物探针组合物及其应用 |
| WO2025240660A1 (en) | 2024-05-15 | 2025-11-20 | Foresite Labs, Llc | Drug development and drug activity determination for coronary artery disease (cad) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000516468A (ja) * | 1996-08-14 | 2000-12-12 | ライフ テクノロジーズ,インコーポレイテッド | 核酸増幅および配列決定のための安定な組成物 |
| US5976842A (en) * | 1997-10-30 | 1999-11-02 | Clontech Laboratories, Inc. | Methods and compositions for use in high fidelity polymerase chain reaction |
| GB9815799D0 (en) * | 1998-07-21 | 1998-09-16 | Pharmagene Lab Limited | Quantitative analysis of gene expression |
-
2002
- 2002-11-13 CA CA2407744A patent/CA2407744C/en not_active Expired - Lifetime
- 2002-11-16 ES ES02025794T patent/ES2260378T3/es not_active Expired - Lifetime
- 2002-11-16 AT AT02025794T patent/ATE319859T1/de active
- 2002-11-16 DE DE60209617T patent/DE60209617T2/de not_active Expired - Lifetime
- 2002-11-16 EP EP02025794A patent/EP1312684B1/de not_active Expired - Lifetime
- 2002-11-19 US US10/300,576 patent/US7118867B2/en not_active Expired - Lifetime
- 2002-11-20 JP JP2002337219A patent/JP3909010B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20030215830A1 (en) | 2003-11-20 |
| EP1312684A2 (de) | 2003-05-21 |
| US7118867B2 (en) | 2006-10-10 |
| EP1312684A3 (de) | 2004-02-04 |
| JP3909010B2 (ja) | 2007-04-25 |
| ES2260378T3 (es) | 2006-11-01 |
| CA2407744A1 (en) | 2003-05-20 |
| CA2407744C (en) | 2012-01-03 |
| EP1312684B1 (de) | 2006-03-08 |
| JP2003210199A (ja) | 2003-07-29 |
| DE60209617D1 (de) | 2006-05-04 |
| DE60209617T2 (de) | 2006-11-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE319859T1 (de) | Quantitative multiplex-pcr mit grossem dynamikbereich | |
| ATE557083T1 (de) | Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität | |
| DE60238493D1 (de) | Is von seltenen zellen verwendete markierte zellen | |
| BR0101493B1 (pt) | mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes. | |
| WO2003040395A3 (en) | Universal nucleotides for nucleic acid analysis | |
| WO2003036265A8 (en) | Assay systems with adjustable fluid communication | |
| EP1436385A4 (de) | Dna-polymerasen und mutanten davon | |
| PT1468114E (pt) | Late-pcr | |
| AU2003296896A8 (en) | Dna polymerase mutants with reverse transcriptase activity | |
| DE69940662D1 (de) | Verfahren zur dna-amplifikation und -sequenzierung | |
| DE50108502D1 (de) | Abdeckmatte | |
| ATE400663T1 (de) | Rekombinase-polymerase-amplifikation | |
| ATE244759T1 (de) | Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation | |
| IS5455A (is) | Skynjun á mispörun DNA para með því að nota flæðigreiningu | |
| ATE278805T1 (de) | Verfahren zur quantifizierung von cholesterol in lipoproteinen und quantifizierungsreagenzien | |
| DE60235005D1 (de) | Nachweis der instabilität von mikrosatelliten und | |
| WO2004097175A3 (fr) | Dispositif d'analyse d'au moins un gaz contenu dans un liquide notamment un fluide de forage | |
| WO2003048377A3 (en) | Multiplex real-time quantitative pcr | |
| DE60141097D1 (de) | Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis | |
| AU2003225062A1 (en) | Thermostable dna polymerases and methods of making same | |
| WO2003048383A3 (en) | Effectors of innate immunity | |
| AU2002352902A8 (en) | Thermus thermophilus nucleic acid polymerases | |
| WO2003066804A3 (en) | Thermus scotoductus nucleic acid polymerases | |
| WO2003048310A3 (en) | Thermus oshimai nucleic acid polymerases | |
| ATE534731T1 (de) | Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäuren |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1312684 Country of ref document: EP |