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ATE306328T1 - APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES - Google Patents

APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES

Info

Publication number
ATE306328T1
ATE306328T1 AT00905525T AT00905525T ATE306328T1 AT E306328 T1 ATE306328 T1 AT E306328T1 AT 00905525 T AT00905525 T AT 00905525T AT 00905525 T AT00905525 T AT 00905525T AT E306328 T1 ATE306328 T1 AT E306328T1
Authority
AT
Austria
Prior art keywords
container
magnetic particles
magnetic
test medium
magnet
Prior art date
Application number
AT00905525T
Other languages
German (de)
Inventor
Iqbal Waheed Siddiqi
Original Assignee
Sigris Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigris Res Inc filed Critical Sigris Res Inc
Application granted granted Critical
Publication of ATE306328T1 publication Critical patent/ATE306328T1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

Abstract

An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
AT00905525T 2000-01-04 2000-01-04 APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES ATE306328T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/000071 WO2001049419A1 (en) 2000-01-04 2000-01-04 Apparatus and method for mixing and separation employing magnetic particles

Publications (1)

Publication Number Publication Date
ATE306328T1 true ATE306328T1 (en) 2005-10-15

Family

ID=21740942

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00905525T ATE306328T1 (en) 2000-01-04 2000-01-04 APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES

Country Status (5)

Country Link
EP (1) EP1248680B1 (en)
JP (1) JP4651894B2 (en)
AT (1) ATE306328T1 (en)
DE (1) DE60023185T2 (en)
WO (1) WO2001049419A1 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1499415A4 (en) 2002-04-26 2009-10-28 Abbott Lab Structure and method for handling magnetic particles in biological assays
CN1280428C (en) * 2003-05-19 2006-10-18 清华大学 Biochip system based on minute particle and its application
WO2008007270A2 (en) * 2006-06-21 2008-01-17 Spinomix S.A. A method for manipulating magnetic particles in a liquid medium
JP4586054B2 (en) * 2007-08-31 2010-11-24 株式会社日立ハイテクノロジーズ Automatic analyzer
GB0717452D0 (en) * 2007-09-07 2007-10-17 Mole Genetics As Separation apparatus
JP2009066476A (en) * 2007-09-11 2009-04-02 Tamagawa Seiki Co Ltd Method and apparatus for collecting / dispersing magnetic particles in a liquid
US7847932B2 (en) 2007-12-28 2010-12-07 Morpho Detection, Inc. System and method for improved biodetection
US7852470B2 (en) 2007-12-28 2010-12-14 Morpho Detection, Inc. System and method for improved biodetection
US8053250B2 (en) * 2008-06-27 2011-11-08 Rex Chin-Yih Hong Method and system for suppressing bindings on magnetic particles
WO2010117458A1 (en) 2009-04-10 2010-10-14 President And Fellows Of Harvard College Manipulation of particles in channels
EP2665807B1 (en) * 2011-03-29 2018-10-10 Zhang, Yongxin Multifunctional bioreactor system and methods for cell sorting and culturing
JP5347121B2 (en) * 2012-02-14 2013-11-20 多摩川精機株式会社 Device for collecting / dispersing magnetic particles in liquid
JP2012152213A (en) * 2012-02-14 2012-08-16 Tamagawa Seiki Co Ltd Device for aggregating/dispersing magnetic particle in liquid
JP2012152734A (en) * 2012-02-14 2012-08-16 Tamagawa Seiki Co Ltd Assembly/dispersion apparatus of magnetic particle in liquid
CN106457196B (en) * 2014-05-23 2019-04-26 株式会社岛津制作所 Method for operating magnetic substance particles and device for manipulating magnetic substance particles
WO2015177934A1 (en) * 2014-05-23 2015-11-26 株式会社島津製作所 Method for operating magnetic body particles and device for operating magnetic body particles
JP2018161649A (en) * 2018-04-10 2018-10-18 株式会社島津製作所 Magnetic particle operation method and magnetic particle operation device
US11406989B2 (en) * 2018-04-25 2022-08-09 Zymo Research Corporation Apparatus and methods centrifugal and magnetic sample isolation
WO2020019001A1 (en) 2018-07-20 2020-01-23 Cornell University Magnetic separation of biological entities from fluid sample
CN110346556B (en) * 2019-07-15 2023-02-17 深圳海思安生物技术有限公司 Liquid detection reagent and using method thereof
CN110423676B (en) * 2019-08-13 2023-04-14 华东理工大学 Magnetically controlled bioreactor system for cell culture in vitro
EP4065260A4 (en) * 2019-11-27 2023-08-02 JBS Science Inc. Method and apparatus for mixing magnetic particles in liquid medium
EP4115177B1 (en) * 2020-03-02 2024-09-11 DH Technologies Development Pte. Ltd. Electromagnetic sampling device protected in a septum piercing needle
EP4126374A1 (en) * 2020-04-03 2023-02-08 Beckman Coulter Inc. Electromagnetic assemblies for processing fluids

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3985649A (en) * 1974-11-25 1976-10-12 Eddelman Roy T Ferromagnetic separation process and material
JPS5753257A (en) * 1980-09-16 1982-03-30 Tohoku Metal Ind Ltd Apparatus for separating magnetic particulate body
JPS58193687A (en) * 1982-05-10 1983-11-11 Res Dev Corp Of Japan Agitation and separation of microscopic material
WO1996026011A1 (en) * 1995-02-21 1996-08-29 Siddiqi Iqbal W Apparatus and method for mixing and separation employing magnetic particles

Also Published As

Publication number Publication date
DE60023185T2 (en) 2006-05-11
DE60023185D1 (en) 2006-02-23
EP1248680B1 (en) 2005-10-12
JP2003519008A (en) 2003-06-17
EP1248680A1 (en) 2002-10-16
JP4651894B2 (en) 2011-03-16
WO2001049419A1 (en) 2001-07-12

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