ATE361308T1 - Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden - Google Patents

Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden

Info

Publication number: ATE361308T1
Authority: AT; Austria
Prior art keywords: molecular weight; low molecular; high affinity; oligonucleotides; purifying oligonucleotides
Prior art date: 1999-06-29

Application number

AT00943241T

Other languages

English (en)

Inventor

Abhinav A Shukla

Ranjit R Deshmukh

Steven M Cramer

James A Moore

Original Assignee

Isis Pharmaceuticals Inc

Rensselaer Polytech Inst

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-06-29

Filing date

2000-06-28

Publication date

2007-05-15

2000-06-28 Application filed by Isis Pharmaceuticals Inc, Rensselaer Polytech Inst filed Critical Isis Pharmaceuticals Inc

2007-05-15 Application granted granted Critical

2007-05-15 Publication of ATE361308T1 publication Critical patent/ATE361308T1/de

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 3
239000003153 chemical reaction reagent Substances 0.000 title 1
238000006073 displacement reaction Methods 0.000 title 1
238000000034 method Methods 0.000 abstract 2
238000005349 anion exchange Methods 0.000 abstract 1
125000000129 anionic group Chemical group 0.000 abstract 1
238000002086 displacement chromatography Methods 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
-1 polycyclic aromatic compounds Chemical class 0.000 abstract 1
238000000926 separation method Methods 0.000 abstract 1
125000000542 sulfonic acid group Chemical group 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Analytical Chemistry (AREA)
Biomedical Technology (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Crystallography & Structural Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Saccharide Compounds (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Detergent Compositions (AREA)

AT00943241T 1999-06-29 2000-06-28 Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden ATE361308T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/343,006 US6573373B1 (en)	1999-06-29	1999-06-29	High affinity, low molecular weight displacers for oligonucleotide purification

Publications (1)

Publication Number	Publication Date
ATE361308T1 true ATE361308T1 (de)	2007-05-15

Family

ID=23344283

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT00943241T ATE361308T1 (de)	1999-06-29	2000-06-28	Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden

Country Status (7)

Country	Link
US (2)	US6573373B1 (de)
EP (1)	EP1192172B1 (de)
JP (1)	JP2003503418A (de)
AT (1)	ATE361308T1 (de)
AU (1)	AU5774000A (de)
DE (1)	DE60034676T2 (de)
WO (1)	WO2001000642A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6573373B1 (en) *	1999-06-29	2003-06-03	Isis Pharmaceuticals, Inc.	High affinity, low molecular weight displacers for oligonucleotide purification
AU2003213642A1 (en) *	2002-02-28	2003-09-16	Rensselaer Polytechnic Institute	High-affinity, low-molecular-mass displacers for ion-exchange chromatography
US8080560B2 (en) *	2004-12-17	2011-12-20	3M Innovative Properties Company	Immune response modifier formulations containing oleic acid and methods
WO2006116064A2 (en) *	2005-04-22	2006-11-02	Waters Investments Limited	Apparatus and methods for mass-spectrometric directed purification of biopolymers
US7994220B2 (en)	2005-09-28	2011-08-09	Cypress Bioscience, Inc.	Milnacipran for the long-term treatment of fibromyalgia syndrome
CN101336124B (zh) *	2005-12-02	2012-06-13	塞克姆公司	阴离子交换顶替色谱法及在阴离子交换顶替色谱法中作为置换剂化合物的阴离子有机化合物
GB201115218D0 (en) *	2011-09-02	2011-10-19	Glaxo Group Ltd	Novel process
TWI432499B (zh)	2011-12-26	2014-04-01	Ind Tech Res Inst	複合原料、碳纖維材料及其形成方法
WO2021111418A1 (en) *	2019-12-05	2021-06-10	Waters Technologies Corporation	Polyphosphonic acids for improving recovery and minimizing system loss

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IT1232311B (it)	1989-09-04	1992-01-28	Sclavo Spa	Metodo di purificazione di composti a struttura peptidica o pseudo peptidica a basso peso molecolare.
US5866429A (en) *	1991-04-03	1999-02-02	Bloch; Will	Precision and accuracy of anion-exchange separation of nucleic acids
CA2106612C (en)	1993-09-21	2001-02-06	Diana Pliura	Displacement chromatography process
US5478924A (en)	1994-02-16	1995-12-26	Cramer; Steven M.	Displacement chromatography of proteins using low molecular weight displacers
US5427686A (en)	1994-04-05	1995-06-27	Sri International	Process for separating material from mixture using displacement chromatography
US6329145B1 (en)	1999-02-09	2001-12-11	Gilead Science, Inc.	Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6573373B1 (en) *	1999-06-29	2003-06-03	Isis Pharmaceuticals, Inc.	High affinity, low molecular weight displacers for oligonucleotide purification

1999
- 1999-06-29 US US09/343,006 patent/US6573373B1/en not_active Expired - Fee Related
2000
- 2000-06-28 EP EP00943241A patent/EP1192172B1/de not_active Expired - Lifetime
- 2000-06-28 JP JP2001507049A patent/JP2003503418A/ja active Pending
- 2000-06-28 DE DE60034676T patent/DE60034676T2/de not_active Expired - Fee Related
- 2000-06-28 WO PCT/US2000/017750 patent/WO2001000642A1/en not_active Ceased
- 2000-06-28 AT AT00943241T patent/ATE361308T1/de not_active IP Right Cessation
- 2000-06-28 AU AU57740/00A patent/AU5774000A/en not_active Abandoned
2002
- 2002-05-15 US US10/146,266 patent/US6828436B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
WO2001000642A1 (en)	2001-01-04
WO2001000642A9 (en)	2002-07-25
EP1192172A1 (de)	2002-04-03
JP2003503418A (ja)	2003-01-28
US20020156267A1 (en)	2002-10-24
DE60034676T2 (de)	2008-01-17
EP1192172B1 (de)	2007-05-02
DE60034676D1 (de)	2007-06-14
EP1192172A4 (de)	2002-08-28
US6828436B2 (en)	2004-12-07
US6573373B1 (en)	2003-06-03
AU5774000A (en)	2001-01-31

Publication	Publication Date	Title
CA2349948A1 (en)	2000-05-18	A chromatography method and a column material useful in said method
BR9404707A (pt)	1995-08-01	Compostos de metaloceno,sistema de catalisador,processos para a preparação de tais compostos e para a preparação de um polímero de olefina,artigo moldado e aplicação dos ditos compostos
ATE361308T1 (de)	2007-05-15	Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden
ATE224908T1 (de)	2002-10-15	Oligomere phosphoramidat- und phosphorthioamidatverbindungen
DK1026239T3 (da)	2011-06-14	Ny fremgangsmåde til at udnytte det immunologiske repertoire
EP1482040A3 (de)	2004-12-08	Nl3 Tie rezeptortyrosinekinase Ligandhomologen
WO1998054228A3 (en)	1999-03-04	Amide containing molecular imprinted polymers
DE60022280D1 (de)	2005-10-06	Verfahren zu Herstellung von gereinigte Tocotrienolen und Tocopherolen mit Hilfe von Flüssigechromatographie
EA199800561A1 (ru)	1999-02-25	Способ деполимеризации полиамидов
BR0015202A (pt)	2002-07-23	Processo para a purificação de uma solução contendo aminoácido por meio de eletrodiálise, e processo integrado para a produção de um aminoácido livre
CA2183160A1 (en)	1995-08-24	Displacement Chromatography of Proteins Using Low Molecular Weight Displacers
MX9603656A (es)	1997-03-29	Procedimiento para hidroformilar compuestos olefinicamente insaturados.
WO2001032684A3 (de)	2002-04-18	Verfahren zur verbindung von molekularen substanzen basierend auf der affinität der prolinreichen aminosäure-sequenzen und proteindomänem vom typ ww
WO2002018929A3 (en)	2002-10-31	Chromatography and other adsorptions using modified carbon adsorbents
AU3273200A (en)	2000-10-04	Ion exchange chromatographic separation of glp-1 and related peptides
EP0636600A4 (de)	1995-07-05	Verfahren zur reinigung aromatischer dicarbonsäuren.
FR2689503B1 (fr)	1994-06-10	Procede d'epuration d'un hydrofluoroalcane.
EP1167418A4 (de)	2002-08-14	Polyoxyethylenderivate mit einer endständigen, geschützten aminogruppe und verfahren zu deren herstellung
AU5255893A (en)	1994-07-07	Process for reactivating purified membrane proteins
EP0832978A3 (de)	1999-11-24	3-Dehydrochinate Synthase
EE9900081A (et)	1999-10-15	Protsess kontrastainete valmistamiseks
MX9603655A (es)	1997-03-29	Procedimiento para la preparacion de pentenaleno.
DK0832870T3 (da)	2001-09-24	Fremgangsmåde til fremstillingen af 3,5,5-trimethylcyclohex-3-en-1-on(beta-isophoron) ved isomerisering af 3,5,5-trimethylcyclohex-2-en-1-on(alfa-isophoron)
WO2002102857A3 (en)	2003-08-14	Catalyst system and its use in a polymerization process
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Legal Events

Date	Code	Title	Description
2007-11-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties