ATE293696T1 - Mutierte alpha-amylase enzyme mit erhöhter stabilität - Google Patents
Mutierte alpha-amylase enzyme mit erhöhter stabilitätInfo
- Publication number
- ATE293696T1 ATE293696T1 AT96945189T AT96945189T ATE293696T1 AT E293696 T1 ATE293696 T1 AT E293696T1 AT 96945189 T AT96945189 T AT 96945189T AT 96945189 T AT96945189 T AT 96945189T AT E293696 T1 ATE293696 T1 AT E293696T1
- Authority
- AT
- Austria
- Prior art keywords
- amino acid
- increased stability
- amylase enzymes
- protein
- mutated alpha
- Prior art date
Links
- 102000004139 alpha-Amylases Human genes 0.000 title 1
- 108090000637 alpha-Amylases Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 125000000539 amino acid group Chemical group 0.000 abstract 2
- 239000002243 precursor Substances 0.000 abstract 2
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1996/019595 WO1998026078A1 (en) | 1996-12-09 | 1996-12-09 | H mutant alpha-amylase enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE293696T1 true ATE293696T1 (de) | 2005-05-15 |
Family
ID=22256262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96945189T ATE293696T1 (de) | 1996-12-09 | 1996-12-09 | Mutierte alpha-amylase enzyme mit erhöhter stabilität |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0942994B1 (de) |
| JP (1) | JP4253041B2 (de) |
| AT (1) | ATE293696T1 (de) |
| AU (1) | AU1461497A (de) |
| CA (1) | CA2274806C (de) |
| DE (1) | DE69634640T2 (de) |
| WO (1) | WO1998026078A1 (de) |
Families Citing this family (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6440716B1 (en) | 1995-02-03 | 2002-08-27 | Novozymes A/S | α-amylase mutants |
| US6080568A (en) * | 1997-08-19 | 2000-06-27 | Genencor International, Inc. | Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis |
| US6197565B1 (en) * | 1998-11-16 | 2001-03-06 | Novo-Nordisk A/S | α-Amylase variants |
| US6887986B1 (en) | 1998-11-16 | 2005-05-03 | Novozymes A/S | α-amylase variants |
| US6268328B1 (en) * | 1998-12-18 | 2001-07-31 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| JP4668426B2 (ja) * | 1999-03-30 | 2011-04-13 | ノボザイムス アクティーゼルスカブ | α−アミラーゼ変異体 |
| US6410295B1 (en) | 1999-03-30 | 2002-06-25 | Novozymes A/S | Alpha-amylase variants |
| EP1067181A1 (de) * | 1999-06-10 | 2001-01-10 | Rijksuniversiteit te Groningen | Enzymselektion |
| AU2099301A (en) * | 1999-12-23 | 2001-07-09 | Genencor International, Inc. | Method for obtaining proteins having improved functional characteristics |
| RU2003105683A (ru) | 2000-07-28 | 2004-08-20 | Хенкель Кгаа (De) | Новый амилолитический фермент из bacillus sp.а7-7(dsm12368), а также моющее и чистящее средство с этим новым амилолитическим ферментом |
| US20020155574A1 (en) | 2000-08-01 | 2002-10-24 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| EP1597363B1 (de) * | 2003-02-26 | 2015-07-15 | Danisco US Inc. | Amylasen, die eine veränderte immunogene reaktion bewirken, und verfahren zu ihrer herstellung und verwendung |
| DE102004047777B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung |
| US7629158B2 (en) | 2006-06-16 | 2009-12-08 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
| GB201011513D0 (en) * | 2010-07-08 | 2010-08-25 | Danisco | Method |
| JP2016519651A (ja) | 2013-03-15 | 2016-07-07 | アンジェリカ セラピューティックス，インク． | 改質された毒素 |
| JP6367930B2 (ja) | 2013-05-29 | 2018-08-01 | ダニスコ・ユーエス・インク | 新規メタロプロテアーゼ |
| EP3110833B1 (de) | 2013-05-29 | 2020-01-08 | Danisco US Inc. | Neuartige metalloproteasen |
| EP3882346B1 (de) | 2013-05-29 | 2025-10-01 | Danisco US Inc. | Neuartige metalloproteasen |
| US20160160202A1 (en) | 2013-05-29 | 2016-06-09 | Danisco Us Inc. | Novel metalloproteases |
| DK3080263T3 (da) | 2013-12-13 | 2019-10-07 | Danisco Us Inc | Serinproteaser af bacillus gibsonii-clade |
| DK3080262T3 (da) | 2013-12-13 | 2019-05-06 | Danisco Us Inc | Serinproteaser af bacillus-arter |
| EP3083704B1 (de) | 2013-12-16 | 2022-08-17 | Nutrition & Biosciences USA 4, Inc. | Verwendung von poly-alpha-1,3-glucan-ethern als viskositätsmodifikatoren |
| KR102410391B1 (ko) | 2013-12-18 | 2022-06-16 | 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. | 양이온성 폴리 알파-1,3-글루칸 에테르 |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| ES2734726T3 (es) | 2014-03-11 | 2019-12-11 | Du Pont | Poli alfa-1,2-glucano oxidado como reforzante de detergente |
| US20170096653A1 (en) | 2014-03-21 | 2017-04-06 | Danisco Us Inc. | Serine proteases of bacillus species |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| EP3919599A1 (de) | 2014-06-19 | 2021-12-08 | Nutrition & Biosciences USA 4, Inc. | Zusammensetzungen mit einer oder mehreren poly-alpha-1,3-glucan-ether-verbindungen |
| WO2016061438A1 (en) | 2014-10-17 | 2016-04-21 | Danisco Us Inc. | Serine proteases of bacillus species |
| WO2016069548A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
| EP3224357A1 (de) | 2014-10-27 | 2017-10-04 | Danisco US Inc. | Serinproteasen aus einer bacillus-spezies |
| EP3212783B1 (de) | 2014-10-27 | 2024-06-26 | Danisco US Inc. | Serinproteasen |
| WO2016069569A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
| DK3212662T3 (da) | 2014-10-27 | 2020-07-20 | Danisco Us Inc | Serinproteaser |
| EP3237631A1 (de) | 2014-12-23 | 2017-11-01 | E. I. du Pont de Nemours and Company | Enzymatisch hergestellte cellulose |
| EP4219704A3 (de) | 2015-05-13 | 2023-08-23 | Danisco US Inc | Aprl-clade-proteasevarianten und verwendungen davon |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| US20180134997A1 (en) | 2015-06-09 | 2018-05-17 | Danisco Us Inc. | Osmotic burst encapsulates |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| WO2016205755A1 (en) | 2015-06-17 | 2016-12-22 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
| US20180320158A1 (en) | 2015-11-05 | 2018-11-08 | Danisco Us Inc. | Paenibacillus and bacillus spp. mannanases |
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| US10876074B2 (en) | 2015-11-13 | 2020-12-29 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
| CN108779448B (zh) | 2015-12-09 | 2023-08-18 | 丹尼斯科美国公司 | α-淀粉酶组合变体 |
| WO2017106676A1 (en) | 2015-12-18 | 2017-06-22 | Danisco Us Inc | Polypeptides with endoglucanase activity and uses thereof |
| WO2017192692A1 (en) | 2016-05-03 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
| CN109072213A (zh) | 2016-05-05 | 2018-12-21 | 丹尼斯科美国公司 | 蛋白酶变体及其用途 |
| JP7609549B2 (ja) | 2016-05-31 | 2025-01-07 | ダニスコ・ユーエス・インク | プロテアーゼ変異体およびその使用 |
| JP7152319B2 (ja) | 2016-06-17 | 2022-10-12 | ダニスコ・ユーエス・インク | プロテアーゼ変異体およびその使用 |
| US20190264138A1 (en) | 2016-11-07 | 2019-08-29 | Danisco Us Inc. | Laundry detergent composition |
| EP3559226B1 (de) | 2016-12-21 | 2023-01-04 | Danisco US Inc. | Bacillus-gibboni-clade-serin-proteasen |
| WO2018118917A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Protease variants and uses thereof |
| EP3583210B1 (de) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsinähnliche serinproteasen und deren verwendungen |
| US20200040283A1 (en) | 2017-03-31 | 2020-02-06 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
| MX2019014556A (es) | 2017-06-30 | 2020-02-07 | Danisco Us Inc | Particulas que contienen enzimas de baja aglomeracion. |
| CN111373039A (zh) | 2017-11-29 | 2020-07-03 | 丹尼斯科美国公司 | 具有改善的稳定性的枯草杆菌蛋白酶变体 |
| CA3086202A1 (en) | 2017-12-21 | 2019-06-27 | Danisco Us Inc. | Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant |
| CN111867388A (zh) | 2018-02-08 | 2020-10-30 | 丹尼斯科美国公司 | 用于酶包封的耐热蜡基质颗粒 |
| EP3810767A1 (de) | 2018-06-19 | 2021-04-28 | Danisco US Inc. | Subtilisin-varianten |
| US20210363470A1 (en) | 2018-06-19 | 2021-11-25 | Danisco Us Inc | Subtilisin variants |
| EP3844255A1 (de) | 2018-08-30 | 2021-07-07 | Danisco US Inc. | Enzymhaltige granulate |
| EP3856882A1 (de) | 2018-09-27 | 2021-08-04 | Danisco US Inc. | Zusammensetzungen für die reinigung von medizinischen instrumenten |
| US20230028935A1 (en) | 2018-11-28 | 2023-01-26 | Danisco Us Inc | Subtilisin variants having improved stability |
| CN114174504A (zh) | 2019-05-24 | 2022-03-11 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| EP3980517A1 (de) | 2019-06-06 | 2022-04-13 | Danisco US Inc. | Verfahren und zusammensetzungen zum reinigen |
| US20240034960A1 (en) | 2020-08-27 | 2024-02-01 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
| US20240117275A1 (en) | 2021-01-29 | 2024-04-11 | Danisco Us Inc. | Compositions for cleaning and methods related thereto |
| CN117616120A (zh) | 2021-06-30 | 2024-02-27 | 丹尼斯科美国公司 | 变体脂肪酶及其用途 |
| CN117916354A (zh) | 2021-09-03 | 2024-04-19 | 丹尼斯科美国公司 | 用于清洁的衣物洗涤组合物 |
| WO2023039270A2 (en) | 2021-09-13 | 2023-03-16 | Danisco Us Inc. | Bioactive-containing granules |
| WO2023114936A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| WO2023114932A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| EP4448749A2 (de) | 2021-12-16 | 2024-10-23 | Danisco US Inc. | Subtilisinvarianten und verfahren zur verwendung |
| US20250163396A1 (en) | 2022-03-01 | 2025-05-22 | Danisco Us Inc. | Enzymes and enzyme compositions for cleaning |
| PE20251535A1 (es) | 2022-05-14 | 2025-06-05 | Novozymes As | Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas |
| EP4544016A1 (de) | 2022-06-21 | 2025-04-30 | Danisco US Inc. | Reinigungsverfahren und -zusammensetzungen mit einem polypeptid mit thermolysinaktivität |
| EP4581138A1 (de) | 2022-09-02 | 2025-07-09 | Danisco US Inc. | Subtilisinvarianten und damit in zusammenhang stehende verfahren |
| EP4581137A1 (de) | 2022-09-02 | 2025-07-09 | Danisco Us Inc | Mannanasevarianten und verfahren zur verwendung |
| CN119855892A (zh) | 2022-09-02 | 2025-04-18 | 丹尼斯科美国公司 | 洗涤剂组合物及其相关方法 |
| EP4615968A1 (de) | 2022-11-09 | 2025-09-17 | Danisco US Inc. | Subtilisinvarianten und verfahren zur verwendung |
| WO2024163584A1 (en) | 2023-02-01 | 2024-08-08 | Danisco Us Inc. | Subtilisin variants and methods of use |
| WO2024186819A1 (en) | 2023-03-06 | 2024-09-12 | Danisco Us Inc. | Subtilisin variants and methods of use |
| WO2024191711A1 (en) | 2023-03-16 | 2024-09-19 | Nutrition & Biosciences USA 4, Inc. | Brevibacillus fermentate extracts for cleaning and malodor control and use thereof |
| WO2025071996A1 (en) | 2023-09-28 | 2025-04-03 | Danisco Us Inc. | Variant cutinase enzymes with improved solubility and uses thereof |
| WO2025085351A1 (en) | 2023-10-20 | 2025-04-24 | Danisco Us Inc. | Subtilisin variants and methods of use |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9407767A (pt) * | 1993-10-08 | 1997-03-18 | Novo Nordisk As | Variante de enzima &-amilase uso da mesma construção de DNA vetor de express o recombinante célula processos para produzir uma &-amilase hibrida e para preparar uma variante de uma &-amilase aditivo detergente e composições detergentes |
| AU685638B2 (en) * | 1994-06-17 | 1998-01-22 | Genencor International, Inc. | Novel amylolytic enzymes derived from the b. licheniformis alpha-amylase, having improved characteristics |
| KR100511499B1 (ko) * | 1995-02-03 | 2005-12-21 | 노보자임스 에이/에스 | 소정 특성을 가지는 알파-아밀라제 돌연변이체를 디자인하는 방법 |
| US5736499A (en) * | 1995-06-06 | 1998-04-07 | Genencor International, Inc. | Mutant A-amylase |
-
1996
- 1996-12-09 AU AU14614/97A patent/AU1461497A/en not_active Abandoned
- 1996-12-09 EP EP96945189A patent/EP0942994B1/de not_active Expired - Lifetime
- 1996-12-09 DE DE69634640T patent/DE69634640T2/de not_active Expired - Lifetime
- 1996-12-09 WO PCT/US1996/019595 patent/WO1998026078A1/en active IP Right Grant
- 1996-12-09 CA CA2274806A patent/CA2274806C/en not_active Expired - Lifetime
- 1996-12-09 AT AT96945189T patent/ATE293696T1/de not_active IP Right Cessation
- 1996-12-09 JP JP52659698A patent/JP4253041B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001506496A (ja) | 2001-05-22 |
| CA2274806C (en) | 2011-02-01 |
| CA2274806A1 (en) | 1998-06-18 |
| EP0942994A1 (de) | 1999-09-22 |
| DE69634640D1 (de) | 2005-05-25 |
| JP4253041B2 (ja) | 2009-04-08 |
| EP0942994B1 (de) | 2005-04-20 |
| WO1998026078A1 (en) | 1998-06-18 |
| DE69634640T2 (de) | 2006-01-19 |
| AU1461497A (en) | 1998-07-03 |
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