ATE280773T1 - Verfahren zur befestigung von oligonukleotiden auf ein substrat - Google Patents

Verfahren zur befestigung von oligonukleotiden auf ein substrat

Info

Publication number: ATE280773T1
Authority: AT; Austria
Prior art keywords: substrate; oligonucleotide primer; containing multiple; glass; glass substrate
Prior art date: 1999-09-02

Application number

AT00955877T

Other languages

English (en)

Inventor

Xiaodong Zhao

Original Assignee

Amersham Biosciences Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-09-02

Filing date

2000-08-24

Publication date

2004-11-15

2000-08-24 Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp

2004-11-15 Application granted granted Critical

2004-11-15 Publication of ATE280773T1 publication Critical patent/ATE280773T1/de

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 8
239000000758 substrate Substances 0.000 title abstract 7
238000000034 method Methods 0.000 title abstract 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
239000011521 glass Substances 0.000 abstract 6
JUIKUQOUMZUFQT-UHFFFAOYSA-N 2-bromoacetamide Chemical compound NC(=O)CBr JUIKUQOUMZUFQT-UHFFFAOYSA-N 0.000 abstract 2
BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 abstract 2
RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 abstract 2
229910000077 silane Inorganic materials 0.000 abstract 2
BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 abstract 1
125000004103 aminoalkyl group Chemical group 0.000 abstract 1
238000004873 anchoring Methods 0.000 abstract 1
238000006243 chemical reaction Methods 0.000 abstract 1
125000002228 disulfide group Chemical group 0.000 abstract 1
229920000592 inorganic polymer Polymers 0.000 abstract 1
239000002184 metal Substances 0.000 abstract 1
238000005935 nucleophilic addition reaction Methods 0.000 abstract 1
229920000620 organic polymer Polymers 0.000 abstract 1
239000000523 sample Substances 0.000 abstract 1
125000003396 thiol group Chemical group [H]S* 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Structural Engineering (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Medicinal Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

AT00955877T 1999-09-02 2000-08-24 Verfahren zur befestigung von oligonukleotiden auf ein substrat ATE280773T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US09/388,702 US6528319B1 (en)	1999-09-02	1999-09-02	Method for anchoring oligonucleotides to a substrate
PCT/US2000/023255 WO2001016152A2 (en)	1999-09-02	2000-08-24	Method for anchoring oligonucleotides to a substrate

Publications (1)

Publication Number	Publication Date
ATE280773T1 true ATE280773T1 (de)	2004-11-15

Family

ID=23535172

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT00955877T ATE280773T1 (de)	1999-09-02	2000-08-24	Verfahren zur befestigung von oligonukleotiden auf ein substrat

Country Status (7)

Country	Link
US (1)	US6528319B1 (de)
EP (1)	EP1210356B1 (de)
AT (1)	ATE280773T1 (de)
AU (1)	AU6800400A (de)
CA (1)	CA2381761A1 (de)
DE (1)	DE60015368T2 (de)
WO (1)	WO2001016152A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20020076832A1 (en) *	2000-06-02	2002-06-20	Pirrung Michael C.	Method of attaching a biopolymer to a solid support
US20030143331A1 (en) *	2001-10-03	2003-07-31	Micrologix Biotech Inc.	Attachment of thiophosphate tethered oligonucleotides to a solid surface
US20040241701A1 (en) *	2001-11-07	2004-12-02	Vaughn Smider	Method for generation of modular polynucleotides using solid supports
DE10159904A1 (de) *	2001-12-06	2003-07-03	Adnagen Ag	Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür
US20050074781A1 (en) *	2003-10-02	2005-04-07	Herbert von Schroeder	Nucleic acid braided J-probes
GB0326073D0 (en)	2003-11-07	2003-12-10	Solexa Ltd	Improvements in or relating to polynucleotide arrays
US7781187B2 (en)	2005-12-30	2010-08-24	Corning Incorporated	Fluorescent dyes
CA2665536C (en)	2006-10-05	2016-02-16	Massachusetts Institute Of Technology	Multifunctional encoded particles for high-throughput analysis
WO2011156434A2 (en)	2010-06-07	2011-12-15	Firefly Bioworks, Inc.	Nucleic acid detection and quantification by post-hybridization labeling and universal encoding

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5118605A (en) *	1984-10-16	1992-06-02	Chiron Corporation	Polynucleotide determination with selectable cleavage sites
US5700637A (en)	1988-05-03	1997-12-23	Isis Innovation Limited	Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5237016A (en) *	1989-01-05	1993-08-17	Siska Diagnostics, Inc.	End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5888819A (en)	1991-03-05	1999-03-30	Molecular Tool, Inc.	Method for determining nucleotide identity through primer extension
SE501439C2 (sv)	1993-06-22	1995-02-13	Pharmacia Lkb Biotech	Sätt och anordning för analys av polynukleotidsekvenser
US5807522A (en)	1994-06-17	1998-09-15	The Board Of Trustees Of The Leland Stanford Junior University	Methods for fabricating microarrays of biological samples
US5770365A (en)	1995-08-25	1998-06-23	Tm Technologies, Inc.	Nucleic acid capture moieties
US5770151A (en)	1996-06-05	1998-06-23	Molecular Dynamics, Inc.	High-speed liquid deposition device for biological molecule array formation
US5837860A (en)	1997-03-05	1998-11-17	Molecular Tool, Inc.	Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds

1999
- 1999-09-02 US US09/388,702 patent/US6528319B1/en not_active Expired - Fee Related
2000
- 2000-08-24 DE DE60015368T patent/DE60015368T2/de not_active Expired - Fee Related
- 2000-08-24 AU AU68004/00A patent/AU6800400A/en not_active Abandoned
- 2000-08-24 AT AT00955877T patent/ATE280773T1/de not_active IP Right Cessation
- 2000-08-24 EP EP00955877A patent/EP1210356B1/de not_active Expired - Lifetime
- 2000-08-24 CA CA002381761A patent/CA2381761A1/en not_active Abandoned
- 2000-08-24 WO PCT/US2000/023255 patent/WO2001016152A2/en not_active Ceased

Also Published As

Publication number	Publication date
AU6800400A (en)	2001-03-26
WO2001016152A2 (en)	2001-03-08
US6528319B1 (en)	2003-03-04
CA2381761A1 (en)	2001-03-08
DE60015368T2 (de)	2006-02-02
WO2001016152A3 (en)	2001-07-26
EP1210356B1 (de)	2004-10-27
DE60015368D1 (de)	2004-12-02
EP1210356A2 (de)	2002-06-05

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Legal Events

Date	Code	Title	Description
2005-04-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties