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AR112775A1 - DIRECT DETECTION OF RESISTANCE MECHANISMS BY MALDI-TOF MASS SPECTROMETRY - Google Patents

DIRECT DETECTION OF RESISTANCE MECHANISMS BY MALDI-TOF MASS SPECTROMETRY

Info

Publication number
AR112775A1
AR112775A1 ARP180102199A ARP180102199A AR112775A1 AR 112775 A1 AR112775 A1 AR 112775A1 AR P180102199 A ARP180102199 A AR P180102199A AR P180102199 A ARP180102199 A AR P180102199A AR 112775 A1 AR112775 A1 AR 112775A1
Authority
AR
Argentina
Prior art keywords
maldi
mass spectrometry
resistance mechanisms
direct detection
tof mass
Prior art date
Application number
ARP180102199A
Other languages
Spanish (es)
Inventor
Conza Jos Alejandro Di
Gabriel Osvaldo Gutkind
Espinosa Roque Arnulfo Figueroa
Original Assignee
Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Univ De Buenos Aires Uba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet, Univ De Buenos Aires Uba filed Critical Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Publication of AR112775A1 publication Critical patent/AR112775A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para la detección directa de mecanismos de resistencia mediante Espectrometría de masas MALDI-TOF. El método comprende preparar diferentes cultivos bacterianos y además utilizar una técnica de ácido sinapínico para la detección de proteína intacta, en donde cada cultivo bacteriano está contenido en una mancha objetivo MALDI. El método también comprende adquirir un espectro de cada cultivo estableciendo el rango de espectro de un espectrómetro de masas, usando un programa de computación para MALDI-TOF, a un rango comprendido entre 17.000 y 50.000 Da. Finalmente, se comparan los diferentes espectros adquiridos teniendo en cuenta un peso teórico de la proteína a detectar.A method for the direct detection of resistance mechanisms by MALDI-TOF mass spectrometry. The method comprises preparing different bacterial cultures and also using a sinapinic acid technique for the detection of intact protein, where each bacterial culture is contained in a MALDI target spot. The method also comprises acquiring a spectrum of each culture by setting the spectrum range of a mass spectrometer, using a computer program for MALDI-TOF, to a range between 17,000 and 50,000 Da. Finally, the different acquired spectra are compared taking into account a theoretical weight of the protein to be detected.

ARP180102199A 2017-08-17 2018-08-02 DIRECT DETECTION OF RESISTANCE MECHANISMS BY MALDI-TOF MASS SPECTROMETRY AR112775A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762547001P 2017-08-17 2017-08-17

Publications (1)

Publication Number Publication Date
AR112775A1 true AR112775A1 (en) 2019-12-11

Family

ID=63452598

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180102199A AR112775A1 (en) 2017-08-17 2018-08-02 DIRECT DETECTION OF RESISTANCE MECHANISMS BY MALDI-TOF MASS SPECTROMETRY

Country Status (2)

Country Link
AR (1) AR112775A1 (en)
WO (1) WO2019034437A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102729119B1 (en) * 2019-08-02 2024-11-13 (재)씨젠의료재단 A Method for Direct Detection of Pathogenic Microbes with Resistance to β-Lactam Antibiotics
WO2021137645A1 (en) * 2019-12-30 2021-07-08 (재)씨젠의료재단 Direct detection method for pathogenic strain having resistance to carbapenem antibiotics
WO2021263123A1 (en) * 2020-06-26 2021-12-30 Thermo Fisher Scientific Oy Rapid mass spectrometric methods for identifying microbes and antibiotic resistance proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9874570B2 (en) 2011-04-21 2018-01-23 Biomerieux, Inc. Method of detecting at least one mechanism of resistance to cephalosporins by mass spectrometry
CA2833454C (en) 2011-04-21 2020-03-10 Biomerieux Inc. Method of detecting at least one mechanism of resistance to cephalosporins by mass spectrometry
EP2867377B1 (en) 2012-07-02 2019-08-21 The Translational Genomics Research Institute Primers, assays and methods for detecting an e. coli subtype
US9618510B2 (en) 2013-03-13 2017-04-11 Meso Scale Technologies, Llc. Assay methods
CZ2013473A3 (en) * 2013-06-20 2014-11-26 Univerzita Karlova v Praze, Lékařská fakulta v Plzni Detection method of beta-lactamases of gram-negative bacteria using mass spectrometry
FR3024465B1 (en) 2014-07-30 2018-03-23 Biomerieux CHARACTERIZATION OF MICROORGANISMS BY MALDI-TOF
EP3449010A1 (en) 2016-04-27 2019-03-06 The Secretary of State for Health Method for detecting carbapenemase-producing bacteria
AU2017288944B2 (en) 2016-06-27 2023-10-26 Becton, Dickinson And Company Compositions, methods, systems and/or kits for detecting antimicrobial resistance in bacteria
KR101850854B1 (en) 2016-10-17 2018-04-20 연세대학교 원주산학협력단 A method for simultaneously detecting and identifying Gram positive bacteria, Gram negative bacteria, Candida species and antibiotics-resistance, and a kit therefor based QuantaMatrix assay Platform

Also Published As

Publication number Publication date
WO2019034437A1 (en) 2019-02-21

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