AR109117A1 - Métodos para transfectar plantas y para reducir eventos de integración aleatoria - Google Patents
Métodos para transfectar plantas y para reducir eventos de integración aleatoriaInfo
- Publication number
- AR109117A1 AR109117A1 ARP170102045A ARP170102045A AR109117A1 AR 109117 A1 AR109117 A1 AR 109117A1 AR P170102045 A ARP170102045 A AR P170102045A AR P170102045 A ARP170102045 A AR P170102045A AR 109117 A1 AR109117 A1 AR 109117A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- plant cell
- cell
- nucleic acid
- polypeptide
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000010354 integration Effects 0.000 title abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 21
- 210000004027 cell Anatomy 0.000 abstract 13
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102100029766 DNA polymerase theta Human genes 0.000 abstract 3
- 101001094659 Homo sapiens DNA polymerase kappa Proteins 0.000 abstract 3
- 101000865085 Homo sapiens DNA polymerase theta Proteins 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 3
- 241000219195 Arabidopsis thaliana Species 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Reivindicación 1: Un método para reducir la integración aleatoria de moléculas de ácido nucleico transfectadas en una célula de planta, comprendiendo dicho método transfectar una célula de planta con una molécula de ácido nucleico, en donde la expresión y/o actividad de POLQ en dicha célula de planta se reduce en comparación con una célula de planta de tipo salvaje. Reivindicación 4: Método para producir una planta que expresa una molécula de ARN o un polipéptido, que comprende transfectar una célula de planta con una molécula de ácido nucleico que codifica dicha molécula de ARN o polipéptido, en donde la expresión y/o actividad de POLQ en dicha célula de planta se reduce en comparación con una célula de planta de tipo salvaje, y generar la planta a partir de dicha célula de planta. Reivindicación 12: Planta o célula de planta en la que la expresión y/o actividad de POLQ en dicha planta o célula de planta está reducida en comparación con una célula de planta de tipo salvaje, en la que dicha planta o célula de planta no es Arabidopsis thaliana. Reivindicación 15: Uso de la planta o célula de planta de acuerdo con la reivindicación 12 o producida de acuerdo con la reivindicación 13, para producir una planta que expresa una molécula de ARN o un polipéptido, en donde la molécula de ácido nucleico que codifica dicha molécula de ARN o polipéptido está integrada mediante recombinación genética específica de sitio o mediante recombinación homóloga en el cromosoma de la célula de planta.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16162322 | 2016-03-24 | ||
| PCT/NL2017/050182 WO2017164738A1 (en) | 2016-03-24 | 2017-03-23 | Methods for transfecting plants and for reducing random integration events |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR109117A1 true AR109117A1 (es) | 2018-10-31 |
Family
ID=55628927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP170102045A AR109117A1 (es) | 2016-03-24 | 2017-07-20 | Métodos para transfectar plantas y para reducir eventos de integración aleatoria |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US11525140B2 (es) |
| EP (1) | EP3433367B1 (es) |
| JP (1) | JP2019509053A (es) |
| KR (1) | KR20190003504A (es) |
| CN (1) | CN109196105B (es) |
| AR (1) | AR109117A1 (es) |
| AU (1) | AU2017237643B2 (es) |
| BR (1) | BR112018069506A2 (es) |
| CA (1) | CA3018306A1 (es) |
| EA (1) | EA039928B1 (es) |
| ES (1) | ES2892873T3 (es) |
| MX (1) | MX2018011534A (es) |
| UA (1) | UA127447C2 (es) |
| WO (1) | WO2017164738A1 (es) |
| ZA (1) | ZA201806572B (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112018069506A2 (pt) | 2016-03-24 | 2019-01-29 | Academisch Ziekenhuis Leiden | métodos para transfectar plantas e para reduzir eventos de integração aleatória |
| US11184475B2 (en) * | 2017-03-29 | 2021-11-23 | Pioneer Corporation | Mobile apparatus, terminal apparatus, information processing system, information processing method, program for mobile apparatus, and program for terminal apparatus |
| EP3704255A1 (en) * | 2017-10-30 | 2020-09-09 | KWS SAAT SE & Co. KGaA | New strategies for precision genome editing |
| EP3502259A1 (en) * | 2017-12-19 | 2019-06-26 | Universiteit Leiden | A combinational strategy for reducing random integration events when transfecting plants |
| JP7761994B2 (ja) | 2017-12-21 | 2025-10-29 | ベースクリック ゲーエムベーハー | クリック修飾ｍＲＮＡ |
| WO2019234129A1 (en) * | 2018-06-05 | 2019-12-12 | KWS SAAT SE & Co. KGaA | Haploid induction with modified dna-repair |
| WO2019234132A1 (en) * | 2018-06-05 | 2019-12-12 | KWS SAAT SE & Co. KGaA | Base editing in polymerase theta deficient plants |
| EP3660164A1 (en) | 2018-11-30 | 2020-06-03 | KWS SAAT SE & Co. KGaA | Screening of germplasm for desired polymorphisms by assays tested on synthetic dna |
| US20230407320A1 (en) * | 2020-10-29 | 2023-12-21 | Pioneer Hi-Bred International, Inc. | Methods and compositions for genome modification |
| CN113881703B (zh) * | 2021-10-11 | 2022-06-21 | 中国人民解放军军事科学院军事医学研究院 | 一种提高cho细胞同源重组效率的方法及其相关产品和应用 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8401780A (nl) | 1984-06-04 | 1986-01-02 | Rijksuniversiteit Leiden En Pr | Werkwijze voor het inbouwen van vreemd dna in het genoom van planten. |
| US5034323A (en) | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| EP0652965A1 (en) | 1992-07-27 | 1995-05-17 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| AU710874B2 (en) | 1995-06-30 | 1999-09-30 | Dna Plant Technology Corporation | Delayed ripening tomato plants |
| GB9703146D0 (en) | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
| GB9710475D0 (en) | 1997-05-21 | 1997-07-16 | Zeneca Ltd | Gene silencing |
| US6452067B1 (en) | 1997-09-19 | 2002-09-17 | Dna Plant Technology Corporation | Methods to assay for post-transcriptional suppression of gene expression |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
| US20040214330A1 (en) | 1999-04-07 | 2004-10-28 | Waterhouse Peter Michael | Methods and means for obtaining modified phenotypes |
| US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
| GB9925459D0 (en) | 1999-10-27 | 1999-12-29 | Plant Bioscience Ltd | Gene silencing |
| WO2002059257A2 (en) | 2000-10-31 | 2002-08-01 | Commonwealth Scientific And Industrial Research Organisation | Method and means for producing barley yellow dwarf virus resistant cereal plants |
| US20050118648A1 (en) | 2001-09-27 | 2005-06-02 | Limin Li | Methods and combinations for gene targeting by homologous recombination |
| US20030175783A1 (en) | 2002-03-14 | 2003-09-18 | Peter Waterhouse | Methods and means for monitoring and modulating gene silencing |
| WO2003078604A2 (en) | 2002-03-14 | 2003-09-25 | Yale University | Tobacco rattle virus vectors and related compositions and methods |
| JP2005519607A (ja) | 2002-03-14 | 2005-07-07 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼーション | 改変された遺伝子サイレンシングｒｎａおよびその使用 |
| US7846906B2 (en) | 2003-02-28 | 2010-12-07 | Isis Pharmaceuticals, Inc. | Modulation of growth hormone receptor expression and insulin-like growth factor expression |
| ES2444001T3 (es) | 2004-10-21 | 2014-02-21 | Venganza Inc. | Procedimientos y materiales para conferir resistencia a plagas y patógenos de plantas |
| KR20100110298A (ko) | 2007-11-26 | 2010-10-12 | 산타리스 팔마 에이/에스 | 안드로겐 수용체를 표적화하는 ｌｎａ 길항제 |
| HRP20240654T1 (hr) * | 2014-10-04 | 2024-08-02 | Ocri B.V. | Postupak pripremanja emulzije, uređaj za pripremu navedene emulzije i vozilo |
| AU2015342749B2 (en) * | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| BR112018069506A2 (pt) | 2016-03-24 | 2019-01-29 | Academisch Ziekenhuis Leiden | métodos para transfectar plantas e para reduzir eventos de integração aleatória |
-
2017
- 2017-03-23 BR BR112018069506A patent/BR112018069506A2/pt not_active Application Discontinuation
- 2017-03-23 EA EA201891919A patent/EA039928B1/ru unknown
- 2017-03-23 ES ES17718137T patent/ES2892873T3/es active Active
- 2017-03-23 US US16/087,199 patent/US11525140B2/en active Active
- 2017-03-23 WO PCT/NL2017/050182 patent/WO2017164738A1/en not_active Ceased
- 2017-03-23 CA CA3018306A patent/CA3018306A1/en active Pending
- 2017-03-23 UA UAA201809638A patent/UA127447C2/uk unknown
- 2017-03-23 EP EP17718137.7A patent/EP3433367B1/en active Active
- 2017-03-23 CN CN201780028985.0A patent/CN109196105B/zh active Active
- 2017-03-23 AU AU2017237643A patent/AU2017237643B2/en active Active
- 2017-03-23 KR KR1020187030387A patent/KR20190003504A/ko not_active Ceased
- 2017-03-23 JP JP2018549971A patent/JP2019509053A/ja active Pending
- 2017-03-23 MX MX2018011534A patent/MX2018011534A/es unknown
- 2017-07-20 AR ARP170102045A patent/AR109117A1/es unknown
-
2018
- 2018-10-03 ZA ZA2018/06572A patent/ZA201806572B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2017237643A1 (en) | 2018-10-11 |
| CA3018306A1 (en) | 2017-09-28 |
| NZ746945A (en) | 2025-03-28 |
| AU2017237643B2 (en) | 2023-06-22 |
| CN109196105A (zh) | 2019-01-11 |
| BR112018069506A2 (pt) | 2019-01-29 |
| MX2018011534A (es) | 2019-07-04 |
| CN109196105B (zh) | 2022-08-05 |
| KR20190003504A (ko) | 2019-01-09 |
| ZA201806572B (en) | 2021-02-24 |
| EA201891919A1 (ru) | 2019-04-30 |
| JP2019509053A (ja) | 2019-04-04 |
| UA127447C2 (uk) | 2023-08-30 |
| EP3433367B1 (en) | 2021-07-14 |
| US20190390208A1 (en) | 2019-12-26 |
| US11525140B2 (en) | 2022-12-13 |
| EP3433367A1 (en) | 2019-01-30 |
| EA039928B1 (ru) | 2022-03-29 |
| WO2017164738A1 (en) | 2017-09-28 |
| ES2892873T3 (es) | 2022-02-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |