AR091457A1 - Tratamiento de celulas pluripotentes - Google Patents
Tratamiento de celulas pluripotentesInfo
- Publication number
- AR091457A1 AR091457A1 ARP130102110A AR091457A1 AR 091457 A1 AR091457 A1 AR 091457A1 AR P130102110 A ARP130102110 A AR P130102110A AR 091457 A1 AR091457 A1 AR 091457A1
- Authority
- AR
- Argentina
- Prior art keywords
- pluripotent cells
- gsk
- inhibitor
- tra1
- ssea
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 5
- 239000003112 inhibitor Substances 0.000 abstract 3
- 230000002255 enzymatic effect Effects 0.000 abstract 2
- 101100257359 Caenorhabditis elegans sox-2 gene Proteins 0.000 abstract 1
- 108010069241 Connexin 43 Proteins 0.000 abstract 1
- 102000001045 Connexin 43 Human genes 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102100039290 Gap junction gamma-1 protein Human genes 0.000 abstract 1
- 101000976622 Homo sapiens Zinc finger protein 42 homolog Proteins 0.000 abstract 1
- 102000013013 Member 2 Subfamily G ATP Binding Cassette Transporter Human genes 0.000 abstract 1
- 108010090306 Member 2 Subfamily G ATP Binding Cassette Transporter Proteins 0.000 abstract 1
- 101100257363 Mus musculus Sox2 gene Proteins 0.000 abstract 1
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 abstract 1
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 abstract 1
- 101001029301 Xenopus tropicalis Forkhead box protein D3 Proteins 0.000 abstract 1
- 102100023550 Zinc finger protein 42 homolog Human genes 0.000 abstract 1
- 150000001875 compounds Chemical group 0.000 abstract 1
- 108010015426 connexin 45 Proteins 0.000 abstract 1
- -1 crypto Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Reivindicación 1: Un método para expandir y diferenciar células pluripotentes; el método comprende las etapas de: a) cultivar células pluripotentes y b) tratar las células pluripotentes con un inhibidor de la actividad enzimática de GSK-3B. Reivindicación 4: El método de conformidad con la reivindicación 3, en donde las células que expresan marcadores de pluripotencia expresan por lo menos uno de los siguientes marcadores de pluripotencia seleccionados del grupo que consiste en: ABCG2, cripto, FoxD3, Connexin43, Connexin45, Oct4, SOX-2, Nanog, hTERT, UTF-1, ZFP42, SSEA-3, SSEA-4, Tra1-60 y Tra1-81. Reivindicación 7: El método de conformidad con la reivindicación 1, en donde las células pluripotentes se tratan con el inhibidor de la actividad enzimática de GSK-3B por aproximadamente 12 a aproximadamente 48 horas. Reivindicación 12: El método de conformidad con la reivindicación 1, en donde el inhibidor de la actividad enzimática de GSK-3B es un compuesto de la fórmula (1).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261741776P | 2012-06-14 | 2012-06-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR091457A1 true AR091457A1 (es) | 2015-02-04 |
Family
ID=49756254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130102110 AR091457A1 (es) | 2012-06-14 | 2013-06-14 | Tratamiento de celulas pluripotentes |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20130337564A1 (es) |
| EP (1) | EP2861723A4 (es) |
| JP (1) | JP2015519085A (es) |
| KR (1) | KR20150030709A (es) |
| CN (1) | CN104603262A (es) |
| AR (1) | AR091457A1 (es) |
| BR (1) | BR112014031424A2 (es) |
| CA (1) | CA2876671A1 (es) |
| MX (1) | MX2014015419A (es) |
| PH (1) | PH12014502748A1 (es) |
| RU (1) | RU2015100900A (es) |
| SG (1) | SG11201408150UA (es) |
| WO (1) | WO2013192005A2 (es) |
| ZA (1) | ZA201500224B (es) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PE20161035A1 (es) | 2013-03-15 | 2016-11-13 | Global Blood Therapeutics Inc | Compuestos y usos de estos para la modulacion de la hemoglobina |
| EP3008170A4 (en) | 2013-06-11 | 2016-11-09 | Harvard College | SC-CELLS AND COMPOSITIONS AND METHOD FOR THE PRODUCTION THEREOF |
| EA201992707A1 (ru) | 2013-11-18 | 2020-06-30 | Глобал Блад Терапьютикс, Инк. | Соединения и их применения для модуляции гемоглобина |
| ES2985135T3 (es) | 2014-12-18 | 2024-11-04 | Harvard College | Procedimientos para generar células ß derivadas de células madre y usos de las mismas |
| CN107614678B (zh) | 2014-12-18 | 2021-04-30 | 哈佛学院校长同事会 | 干细胞来源的β细胞的产生方法及其使用方法 |
| US10443042B2 (en) | 2014-12-18 | 2019-10-15 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof |
| SG10202101478YA (en) * | 2016-08-18 | 2021-03-30 | Nat Univ Singapore | Substituted azole derivatives for generation, proliferation and differentiation of hematopoietic stem and progenitor cells |
| CN110167455B (zh) | 2016-11-10 | 2022-10-11 | 韦尔赛特公司 | 含有pdx1胰腺内胚层细胞的细胞递送装置及其方法 |
| SG11201903428XA (en) | 2016-11-16 | 2019-05-30 | Cynata Therapeutics Ltd | Pluripotent stem cell assay |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| IL270453B2 (en) | 2017-05-05 | 2025-05-01 | Fusion Pharmaceuticals Inc | Pharmacokinetic enhancements of bifunctional chelates and uses thereof |
| BR112019023249A8 (pt) | 2017-05-05 | 2021-02-23 | Centre For Probe Dev And Commercialization | anticorpos monoclonais igf-1r e usos dos mesmos |
| US10093741B1 (en) | 2017-05-05 | 2018-10-09 | Fusion Pharmaceuticals Inc. | IGF-1R monoclonal antibodies and uses thereof |
| KR101966523B1 (ko) * | 2017-05-29 | 2019-04-05 | 차의과학대학교 산학협력단 | 오가노이드 배양을 위한 조성물 및 방법 |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| MX2020004830A (es) | 2017-11-08 | 2020-11-11 | Avexis Inc | Medios y metodo para preparar vectores virales y usos de los mismos. |
| CA3081762A1 (en) | 2017-11-15 | 2019-05-23 | Semma Therapeutics, Inc. | Islet cell manufacturing compositions and methods of use |
| CN120718958A (zh) | 2018-06-08 | 2025-09-30 | 诺华股份有限公司 | 用于测量药物产品效力的基于细胞的测定 |
| CA3108275A1 (en) | 2018-08-10 | 2020-02-13 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
| US12378572B2 (en) | 2018-09-07 | 2025-08-05 | Crispr Therapeutics Ag | Universal donor cells |
| EP4025690A1 (en) | 2019-09-05 | 2022-07-13 | CRISPR Therapeutics AG | Universal donor cells |
| BR112022004031A2 (pt) | 2019-09-05 | 2022-08-16 | Crispr Therapeutics Ag | Células doadoras universais |
| US11578309B2 (en) | 2020-12-31 | 2023-02-14 | Crispr Therapeutics Ag | Universal donor cells |
| JP2025541369A (ja) * | 2022-12-15 | 2025-12-18 | 睿健毅聯医薬科技（成都）有限公司 | ピロロピリジン誘導体及びその医薬組成物と応用 |
| WO2025117331A1 (en) | 2023-12-01 | 2025-06-05 | Eli Lilly And Company | Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060030042A1 (en) * | 2003-12-19 | 2006-02-09 | Ali Brivanlou | Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime |
| AU2006292021B2 (en) * | 2005-09-12 | 2012-05-31 | Es Cell International Pte Ltd. | Cardiomyocyte production |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US8623648B2 (en) * | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
| US7939322B2 (en) * | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
-
2013
- 2013-06-13 CN CN201380031065.6A patent/CN104603262A/zh active Pending
- 2013-06-13 RU RU2015100900A patent/RU2015100900A/ru not_active Application Discontinuation
- 2013-06-13 US US13/917,109 patent/US20130337564A1/en not_active Abandoned
- 2013-06-13 CA CA2876671A patent/CA2876671A1/en not_active Abandoned
- 2013-06-13 SG SG11201408150UA patent/SG11201408150UA/en unknown
- 2013-06-13 EP EP13806895.2A patent/EP2861723A4/en not_active Withdrawn
- 2013-06-13 MX MX2014015419A patent/MX2014015419A/es unknown
- 2013-06-13 KR KR1020157000636A patent/KR20150030709A/ko not_active Withdrawn
- 2013-06-13 BR BR112014031424A patent/BR112014031424A2/pt not_active IP Right Cessation
- 2013-06-13 WO PCT/US2013/045617 patent/WO2013192005A2/en not_active Ceased
- 2013-06-13 JP JP2015517419A patent/JP2015519085A/ja active Pending
- 2013-06-14 AR ARP130102110 patent/AR091457A1/es unknown
-
2014
- 2014-12-09 PH PH12014502748A patent/PH12014502748A1/en unknown
-
2015
- 2015-01-13 ZA ZA2015/00224A patent/ZA201500224B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015519085A (ja) | 2015-07-09 |
| WO2013192005A3 (en) | 2014-03-13 |
| RU2015100900A (ru) | 2016-08-10 |
| PH12014502748A1 (en) | 2015-02-02 |
| KR20150030709A (ko) | 2015-03-20 |
| WO2013192005A2 (en) | 2013-12-27 |
| MX2014015419A (es) | 2015-07-14 |
| ZA201500224B (en) | 2017-09-27 |
| SG11201408150UA (en) | 2015-01-29 |
| US20130337564A1 (en) | 2013-12-19 |
| CA2876671A1 (en) | 2013-12-27 |
| EP2861723A2 (en) | 2015-04-22 |
| CN104603262A (zh) | 2015-05-06 |
| BR112014031424A2 (pt) | 2017-06-27 |
| EP2861723A4 (en) | 2016-01-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |