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AR090303A1 - MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY - Google Patents

MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY

Info

Publication number
AR090303A1
AR090303A1 ARP130100783A ARP130100783A AR090303A1 AR 090303 A1 AR090303 A1 AR 090303A1 AR P130100783 A ARP130100783 A AR P130100783A AR P130100783 A ARP130100783 A AR P130100783A AR 090303 A1 AR090303 A1 AR 090303A1
Authority
AR
Argentina
Prior art keywords
methods
polipeptide
cepa
muted
understands
Prior art date
Application number
ARP130100783A
Other languages
Spanish (es)
Inventor
Marta Ibaez Maria
Cerminati Sebastian
Carlos Soncini Fernando
Karina Checa Susana
Original Assignee
Consejo Nac Invest Cient Tec
Univ Nac De Rosario Unr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nac Invest Cient Tec, Univ Nac De Rosario Unr filed Critical Consejo Nac Invest Cient Tec
Priority to ARP130100783A priority Critical patent/AR090303A1/en
Priority to PCT/IB2014/059629 priority patent/WO2014141066A2/en
Publication of AR090303A1 publication Critical patent/AR090303A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Polipéptido mutado, cepas que lo comprende y métodos para detectar cationes metálicos monovalentes y divalentes, en donde el polipéptido comprende una sustitución de serina por cisteína en la posición 77 de la SEQ ID Nº 1 o SEQ ID Nº 2; y en donde dicha proteína tiene actividad de regulador transcripcional, por ejemplo GoIS o CueR. El polipéptido detecta cationes metálicos monovalentes y divalentes, por ejemplo Au, Cu, Ag, Hg, Pb, Cd, Co y Zn.Mutated polypeptide, strains comprising it and methods for detecting monovalent and divalent metal cations, wherein the polypeptide comprises a substitution of serine with cysteine at position 77 of SEQ ID No. 1 or SEQ ID No. 2; and wherein said protein has transcriptional regulator activity, for example GoIS or CueR. The polypeptide detects monovalent and divalent metal cations, for example Au, Cu, Ag, Hg, Pb, Cd, Co and Zn.

ARP130100783A 2013-03-11 2013-03-11 MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY AR090303A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ARP130100783A AR090303A1 (en) 2013-03-11 2013-03-11 MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY
PCT/IB2014/059629 WO2014141066A2 (en) 2013-03-11 2014-03-11 Mutated polypeptide, strain of bacteria containing same, and methods for simultaneously detecting different metal cations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP130100783A AR090303A1 (en) 2013-03-11 2013-03-11 MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY

Publications (1)

Publication Number Publication Date
AR090303A1 true AR090303A1 (en) 2014-11-05

Family

ID=50680076

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130100783A AR090303A1 (en) 2013-03-11 2013-03-11 MUTED POLIPEPTIDE, BACTERIA CEPA THAT UNDERSTANDS IT AND METHODS TO DETECT DIFFERENT METALLIC CATIONS SIMULTANEOUSLY

Country Status (2)

Country Link
AR (1) AR090303A1 (en)
WO (1) WO2014141066A2 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE520302C2 (en) * 1997-09-15 2003-06-24 Vito Metal-specific capacitance sensor
AU2002211647A1 (en) * 2000-10-12 2002-04-22 University Of Georgia Research Foundation, Inc. Metal binding proteins, recombinant host cells and methods
CA2559760A1 (en) * 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
BRPI0609460A2 (en) * 2005-03-30 2010-04-13 Novartis Vaccines & Diagnostics Inc haemophilus influenzae type b
CN102250819A (en) * 2010-05-18 2011-11-23 天津工业生物技术研究所 Highly-sensitive biosensor cell for detecting heavy metal mercury and manufacturing method thereof

Also Published As

Publication number Publication date
WO2014141066A3 (en) 2014-12-31
WO2014141066A2 (en) 2014-09-18

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