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AR047142A1 - Composiciones de maiz ricas en lisina y metodos de deteccion de las mismas - Google Patents

Composiciones de maiz ricas en lisina y metodos de deteccion de las mismas

Info

Publication number
AR047142A1
AR047142A1 ARP040104629A ARP040104629A AR047142A1 AR 047142 A1 AR047142 A1 AR 047142A1 AR P040104629 A ARP040104629 A AR P040104629A AR P040104629 A ARP040104629 A AR P040104629A AR 047142 A1 AR047142 A1 AR 047142A1
Authority
AR
Argentina
Prior art keywords
dna
corn
segment
lisina
detection methods
Prior art date
Application number
ARP040104629A
Other languages
English (en)
Inventor
Rebecca A Kelly
Dale A Voyles
Michael G Luethy
Kathleen A Malloy
Mark A Dizigan
Thomas Malvar
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR047142A1 publication Critical patent/AR047142A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254Tryptophan or lysine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684Zea mays [maize]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Ensayos para detectar la presencia de un evento transgénico que eleva el nivel de lisina basados en la secuencia de ADN de una construccion de ADN exogeno insertada en el genoma de maíz y de las genomicas que flanquean al sitio de insercion. También se proveen plantas transgénicas que contienen a la nueva construccion de ADN exogeno que expresa un ácido dihidrodipicolínico sintetasa, cuya actividad da como resultado un mayor nivel de lisina en una planta o el producto de una planta. Reivindicacion 1: Una construccion de ADN, caracterizada porque comprende, en una disposicion ligada operativamente: a) un primer segmento de ADN que comprende un promotor de la globulina 1 de maíz; b) un segundo segmento de ADN que comprende un intron de actina 1 de arroz; c) un tercer segmento de ADN que comprende una molécula de ADN que codifica un péptido de tránsito a cloroplastos de DHDPS de maíz; d) un cuarto segmento de ADN que comprende una molécula de ADN que codifica DHDPS de Corynebacterium; y e) un quinto segmento de ADN que comprende una region no traducida 3' de globulina 1 de maíz.
ARP040104629A 2003-12-11 2004-12-10 Composiciones de maiz ricas en lisina y metodos de deteccion de las mismas AR047142A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US52918203P 2003-12-11 2003-12-11

Publications (1)

Publication Number Publication Date
AR047142A1 true AR047142A1 (es) 2006-01-11

Family

ID=34710111

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP040104629A AR047142A1 (es) 2003-12-11 2004-12-10 Composiciones de maiz ricas en lisina y metodos de deteccion de las mismas
ARP110101663A AR081191A2 (es) 2003-12-11 2011-05-13 Planta transgenica de maiz

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP110101663A AR081191A2 (es) 2003-12-11 2011-05-13 Planta transgenica de maiz

Country Status (18)

Country Link
US (3) US7157281B2 (es)
EP (1) EP1697528B1 (es)
JP (1) JP5633999B2 (es)
KR (2) KR100863376B1 (es)
CN (2) CN102115761B (es)
AR (2) AR047142A1 (es)
AT (1) ATE489469T1 (es)
AU (1) AU2004303836C1 (es)
BR (1) BRPI0417541B1 (es)
CA (1) CA2547323C (es)
DE (1) DE602004030277D1 (es)
MY (2) MY142780A (es)
PE (4) PE20171797A1 (es)
TR (2) TR200800453T1 (es)
TW (1) TWI363798B (es)
UA (2) UA93654C2 (es)
WO (1) WO2005061720A2 (es)
ZA (1) ZA200604589B (es)

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