OA18732A - Therapeutic compositions for treatment of human immunodeficiency virus. - Google Patents

Abstract

A solid oral dosage form is provided, comprising a compound of Formula I <img file="OA18732A_A0001.tif"/> or a pharmaceutically acceptable salt thereof, tenofovir alafenamide or a pharmaceutically acceptable salt thereof, and emtricitabine or a pharmaceutically acceptable salt thereof.

Description

THERAPEUTIC COMPOSITIONS FOR TREATMENT OF HUMAN IMMUNODEFICIENCY VIRUS

TECHNICAL FIELD

[0001] Pharmaceutical formulations suitable for treating viral infections such as HTV are provided, in particular solid oral dosage forms including the compound of Formula I, emtricitabine and tenofovir alafenamide.

BACKGROUND |0002] Human immunodeficiency virus, type 1 (HIV-1) infection is a life-threatening and serions disease of major public health significance, with approximately 35 million people infected worldwide (Joint United Nations Programme on HTV/AIDS (UNAIDS). Global report: UNAIDS report on the global AIDS épidémie, 2013). Standard of care for the treatment of HIV-1 infection uses combination antirétroviral therapy (ART) to suppress viral réplication to below détectable limits, increase CD4 cell counts, and hait disease progression.

[0003] The success of potent and well-tolerated ART means that morbidity and mortality in the HTVinfected population is increasingly driven by non-AIDS associated comorbidities. Clinical attention has become more focused on optimizing tolerability, long-term safety, and adhérence (Costagliola D. Demographics of HTV and aging. Curr. Opin. HIV AIDS, 2014,9(4), 294). There remains a significant medical need for safe and effective new thérapies that take into considération the aging patient population, non-HIV-related comorbidities, virologie résistance, and regimen simplification.

[0004] There is also a need for médications to serve populations with limited treatment options (e.g., children, women, and the elderly). In certain situations, these populations may hâve difficulty maintaining treatment because of pill burden (number of piils to take each day, as well as different combinations of piils) or the size of the piils themselves, once they are coformulated into a multidrug composition. For example, there is currently no fixed dose combination registered for once a day dosing (i.e., QD) for very young children (e.g., younger than âge 12 years).

[0005] A goal of antirétroviral therapy is to achieve viral suppression in the HTV infected patient. Treatment guidelines published by the United States Department of Health and Human Services provide that achievement of viral suppression requires the use of combination thérapies, i.e., several drugs from at least two or more drug classes. ïn addition, decisions regarding the treatment of HIV infected patients are complicated when the patient requires treatment for other medical conditions (e.g., metformin, rifampin, HCV antivirals, hormonal contraceptives, etc.). Because the standard of care requires the use of multiple different drugs to suppress HIV, as well as to treat other conditions the patient may be experiencing, the potential for drug-drug interaction is a criterion for sélection of a drug regimen. As such, there is a need for antirétroviral thérapies having a decreased potential for drug-drug interactions (e.g., those that affect transporters (e.g., OCT-2) or activate receptors (e.g., PXR).

SUMMARY

[0006] Ail the compositions and oral dosage forms herein include a compound of Formula I, (2R,5S, 13aR)-8-hydroxy-7,9-dioxo-N-(2,4,6-trifluorobenzyl)-2,3,4,5,7,9, !3,l3a-octahydro-2,5methanopyrido[r,2':4,5]pyrazino[2,l-b][l,3]oxazepine-l0-carboxamide, having the following structure:

(D

or a pharmaceutically acceptable sait thereof.

|0007) In certain embodiments, the pharmaceutically acceptable sait of the compound of Formula I is a compound of Formula Π, sodium (2R,5S,l3aR)-7,9-dioxo-l0-((2,4,6-trifluorobenzyl)carbamoyl)2,3,4,5,7,9,13,13a-octahydro-2,5-methanopyrido[ 1 ',2':4,5]pyrazîno[2,1 -b][ 1,3]oxazepin-8-olate, having the following structure:

[0008] The inventors hâve successfuliy formulated an oral dosage form containing the compound of Formula I, tenofovir alafenamide and emtricitabine. This oral dosage form is suitable for use in medicine, and in particular in treating viral infections such as HIV.

[0009| In one aspect, a solid oral dosage form comprising the compound of Formula I or a pharmaceutically acceptable sait thereof, tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof is provided. In certain embodiments, the dosage form comprises 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine. For instance, in certain embodiments, the dosage form comprises 50 mg of the 2 compound of Formula I as a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide as a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine. In certain embodiments, the dosage form comprises 52 mg of the compound of Formula Π, 28 mg tenofovir alafenamide hemifumarate, and 200 mg emtricitabine.

[0010] In another aspect, a solid oral dosage form comprising 75 mg of the compound of Formula I as a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide as a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine is provided. In certain embodiments, a solid oral dosage form comprising 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine is provided. In certain embodiments, a solid oral dosage form comprising 78 mg of the compound of Formula Π, 28 mg tenofovir alafenamide hemifumarate, and 200 mg emtricitabine is provided.

[0011] The inventors hâve found that it is possible to formulate solid oral dosage forms that are pharmaceutically acceptable (i.e. pharmacologically efficacious and physically acceptable) while reducing the total amount of excipients necessary to achieve an acceptable pharmacokinetic profile. Accordingly, in one aspect a solid oral dosage form is provided, comprising 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, wherein the dosage form has a total weight of less than 850 mg (e.g. less than 800 mg or less than 730 mg or less than 700 mg).

[0012] In another one aspect a solid oral dosage form is provided, comprising 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, wherein the dosage form has a total weight of less than 850 mg (e.g. less than 800 mg or less than 700 mg).

[0013] In another aspect, a coated tablet comprising 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine or a pharmaceutically acceptable sait thereof is provided.

[0014] In another aspect, a coated tablet comprising 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine or a pharmaceutically acceptable sait thereof îs provided.

[0015] In another aspect, a tablet comprising 52 mg of the compound of Formula Π, 28 mg tenofovir alafenamide hemifumarate, and 200 mg emtricitabine is provided.

[0016] In another aspect, a tablet comprising 78 mg of the compound of Formula II, 28 mg tenofovir alafenamide hemifumarate, and 200 mg emtricitabine is provided.

[0017] In another aspect, a tablet comprising (a) 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) 200 mg emtricitabine or a pharmaceutically acceptable sait thereof is provided, wherein (a) and (b) are segregated, and wherein the tablet has a total weight of less than about 1.5 g (e.g., less than about 1 g). Typically, (a) and (b) are present within separate layers in a multilayer tablet.

[0018] In another aspect, a tablet comprising (a) 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) 200 mg emtricitabine or a pharmaceutically acceptable sait thereof is provided, wherein (a) and (b) are segregated, and wherein the tablet has a total weight of less than about 1.5 g (e.g., less than about 1 g). Typically, (a) and (b) are present within separate layers in a multilayer tablet.

[0019] In another aspect, a tablet comprising from 6.5-11.0 % w/w of the compound of Formula I or a pharmaceutically acceptable sait thereof, 3.0-4.5 % w/w tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 25-30 % w/w emtricitabine or a pharmaceutically acceptable sait thereof is provided, where the weight percentages dénoté a proportion of the whole tablet. In some embodiments, (a) the compound of Formula I is present as of the compound of Formula II and/or (b) the tenofovir alafenamide is present as tenofovir alafenamide hemifumarate.

[0020] In another aspect, a tablet comprising from 9.5-11.5 % w/w ofthe compound of Formula I or a pharmaceutically acceptable sait thereof, 2.5-4.5 % w/w tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 26-33 % w/w emtricitabine or a pharmaceutically acceptable sait thereof is provided, where the weight percentages dénoté a proportion of the whole tablet. In some embodiments, (a) the compound of Formula I is present as of the compound of Formula Π and/or (b) the tenofovir alafenamide is present as tenofovir alafenamide hemifumarate.

]0021] The inventors hâve found that the use of a fixed dose combination may assist in achieving appropriate pharmacokinetic parameters and/or adéquate tablet stability. In addition, the use of a multilayer tablet as a particular type of fixed dose combination may also provide pharmacokinetic and/or stability benefits. Accordingly, in another aspect a fixed dose combination tablet comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) emtricitabine or a pharmaceutically acceptable sait thereof is provided. Additionally, multilayer tablet comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) emtricitabine or a pharmaceutically acceptable sait thereof is provided.

[0022| In another aspect, a kit comprising (a) a tablet comprising the compound of Formula I or a pharmaceutically acceptable sait thereof, tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof, and (b) a desiccant (e.g. silica gel) is provided.

[0023] Methods of producing solid oral dosage forms such as tablets are also provided, as discussed in more detail below.

[0024] In addition, methods for treating patients are provided, which are also discussed in more detail below.

BRIEF DESCRIPTION OF DRAWINGS (0025| Figure I shows the results of studies carried out on Formulations Fl, F2, and F3 to assess the dissolution of 78 mg of the Compound of Formula Π as a single agent compared to a bi-layer using fasted simulated intestinal fluid as a dissolution medium.

[0026] Figure 2 shows the results of studies carried out on Formulations Fl and F2 to assess the dissolution of 78 mg of the Compound of Formula Π as a single agent compared to a bi-layer using fed simulated intestinal fluid as a dissolution medium.

[0027] Figure 3 shows the results of studies carried out on Formulations Fl, F2, F4, F5, and F6 to assess the dissolution of 78 mg of the Compound of Formula II in tablets containing various excipients.

[0028| Figure 4 shows the results of studies carried out on Formulations F7 and F8 to assess the dissolution of 52 mg of the Compound of Formula Π as a single agent compared to a bi-layer using fasted simulated intestinal fluid as a dissolution medium.

[0029] Figure 5 is a flow diagram illustrating the préparation of a tablet formulation containing the compound of Formula Π.

[0030] Figure 6 is a flow diagram illustrating the préparation of a tablet formulation containing the compound of Formula II, emtricitabine, and tenofovir alafenamide hemifumarate.

DETAILED DESCRIPTION

[0031] Typically, the oral dosage forms disclosed herein comprise three active pharmaceutical ingrédients: the compound of Formula I (or a pharmaceutically acceptable sait thereof), tenofovir alafenamide (or a pharmaceutically acceptable sait thereof), and emtricitabine (or a pharmaceutically acceptable sait thereof).

(2R, 5S, 13aR)-8-Hydroxy- 7,9-dioxo-N-(2,4,6-trijluorobenzyl)-2,3,4,5,7,9,13,13a~octahydro-2,5methanopyrido[l',2':4,5[pyrazino[2,l-bHl,3}oxazepine-10-€arboxamide

[00321 (2R,5S, 13aR)-8-Hydroxy-7,9-dioxo-N-(2,4,6-tnfluorobenzyl)-2,3,4,5,7,9, 13,13a-octahydro2,5-methanopyrido[r,2':4,5]pyrazino[2,l-b][l,3]oxazepine-10-carboxamide (Formula I), is a potent HIV integrase inhibitor with in vitro activity against wild type HTV-1. It has the following formula (see WO2014/100323):

|0033[ Its IUP AC name is (2R,5S,13aR)-8-hydroxy-7,9-dioxo-N-(2,4,6-trifluorobenzyl)2,3,4,5,7,9,13,13a-octahydro-2,5-methanopyrido[ 1 ',2':4,5]pyrazino[2,1 -b] [ I,3]oxazepine-l 0carboxamide. Its CAS name is 2,5-Methanopyrido[r,2':4,5]pyrazino[2,l-b][l,3]oxazepine-10carboxamide, 2,3,4,5,7,9,13,13a-octahydro-8-hydroxy-7,9-dioxo-N-[(2,4,6-trifluorophenyl)methyl]-, (2R,5S,I3aR). The compound of Formula I is a weak acid with pKa of 8.6. The aqueous solubility of the compound of Formula I free acid is pH-dependent, with solubility increasing with increasing pH, with a maximum atpH 10.5.The Chemical stability ofthe compound ofFormulai is alsopH dépendent, with maximum stability at pH 4. The compound of Formula I is considered a BCS Class 2 compound, with low solubility and high permeability.

[0034| Solid oral dosage forms disclosed herein include the compound of Formula I, usually in the form of a pharmaceutically acceptable sait. The compound of Formula I can be présent within an oral dosage form in solvated or unsolvated form, and references to “Formula I” include both of these forms. Typically, the compound ofFormula I is in the form of the compound of Formula Π, having the formula below:

[0035J In certain spécifie embodiments, solid oral dosage forms containing 50 mg of the compound of Formula I, e.g. as about 52 mg of the compound of Formula II, are provided.

[0036] In certain spécifie embodiments, solid oral dosage forms containing 75 mg of the compound of Formula I, e.g. as about 78 mg of the compound of Formula Π, are provided.

[0037] As used herein, and in the absence of a spécifie reference to a particular pharmaceutically acceptable sait and/or solvaté of the compound of Formula I (e.g. Formula Π), any dosages, whether expressed in e.g. milligrams or as a % by weight, should be taken as referring to the amount of the compound of Formula I free acid, i.e. the amount of:

[0038] For example, therefore, a reference to “50 mg of the compound of Formula I or a pharmaceutically acceptable sait and/or solvaté thereof ’ means an amount ofthe compound ofFormula I or a pharmaceutically acceptable sait and/or solvaté thereof which provides the same amount of the compound of Formula I as 50 mg of the compound of Formula I free acid.

[0039] For example, therefore, a reference to “75 mg of the compound of Formula I or a pharmaceutically acceptable sait and/or solvaté thereof’ means an amount ofthe compound of Formula I or a pharmaceutically acceptable sait and/or solvaté thereof which provides the same amount of the compound of Formula I as 75 mg of the compound of Formula I free acid.

Tenofovir alafenamide

[00401 Tenofovir alafenamide (TAF) is a nucléotide reverse transcriptase inhibitor having the formula below (see W002/08241 A2):

[00411 Its IUP AC name is (S)-isopropyl-2-(((5)-((((/?)-l-(6-amino-9/7-purin-9-yI)propan-2yl)oxy)methyl)(phenoxy)phosphoryl)amino)propanoate. It is also referred to as {9-[(R)-2-[[(S)-[[(S)l-(isopropoxycarbonyl)ethyl] amino]phenoxyphosphinyl]-methoxy]propyl]adenine). Tenofovir alafenamide is a weak base, with a pKa of 3.9. Its solubility increases with decreasing pH, with a maximum solubility at about pH 3. Tenofovir alafenamide is considered a BCS Class 3 compound, with high equilibrium solubility and lower apparent permeability.

[00-421 Solid oral dosage forms disclosed herein include tenofovir alafenamide, usually in the form of a pharmaceutically acceptable sait. Tenofovir alafenamide can be present within an oral dosage form in solvated or unsolvated form, and references to “tenofovir alafenamide” include both of these forms. In particular, tenofovir alafenamide may be associated with fumarate, such as monofumarate or hemifumarate. Typically, tenofovir alafenamide is in the form of tenofovir alafenamide hemifumarate having the formula below (see WO 2013/025788 Al ):

[0043] As used herein, and in the absence of a spécifie reference to a particular pharmaceutically acceptable sait and/or solvaté of tenofovir alafenamide, any dosages, whether expressed in e.g. milligrams or as a % by weight, should be taken as referring to the amount of tenofovir alafenamide,

i.e. the amount of:

[0044] For example, therefore, a reference to “25 mg tenofovir alafenamide or a pharmaceutically acceptable sait and/or solvaté thereof’ means an amount of tenofovir alafenamide or a pharmaceutically acceptable sait and/or solvaté thereof which provides the same amount of tenofovir alafenamide as 25 mg of tenofovir alafenamide free base.

[0045] The amount of tenofovir alafenamide in a solid oral dosage form provided herein is generally between 10 mg and 30 mg, for instance within the range of 20 mg to 30 mg, and more typically between 24 mg and 28 mg. In certain spécifie embodiments, solid oral dosage forms containing 25 mg of tenofovir alafenamide e.g. as about 28 mg of tenofovir alafenamide hemifumarate, are provided.

Emtricitabine

[0046] Emtricitabine (FTC) is a nucleoside reverse transcriptase inhibitor having the formula below:

[0047] Its IUP AC name is 4-amino-5-fluoro-l-[(2J?,55)-2-(hydroxymethyI)-l,3-oxathiolan-5-yl]-l,2dihydropyrimidin-2-one. It is also referred to as 5-fluoro-l-[(2R,5S)-2-(hydroxymethyl)-l,3oxathiolan-5-yl]cytosine. It is currently authorised as part of EMTRTVA® (emtricitabine 200 mg), TRUVADA® (emtricitabine 200 mg, tenofovir disoproxil fumarate 300 mg), ATRIPLA® (emtricitabine 200 mg, efavirenz 600 mg, tenofovir disoproxil fumarate 300 mg) and STRIBILD® (emtricitabine 200 mg, cobicistat 150 mg, tenofovir disoproxil fumarate 300 mg, elvitegravir 150 mg) and COMPLERA®/EVIPLERA® (rilpivirine 25 mg, emtricitabine 200 mg, tenofovir disoproxil fumarate 300 mg).

[0048] Emtricitabine is a free base, exhibitîng a pKa of 2.65. Solubility is enhanced under acidic conditions. It is considered a BCS Class l compound, with high solubility and high permeability.

[0049] Solid oral dosage forms disclosed herein include emtricitabine, optionally as a pharmaceutically acceptable sait. Emtricitabine can be présent within an oral dosage form in solvated or unsolvated form, and references to “emtricitabine” include both of these forms. Typically, emtricitabine is présent as a free base.

[0050| As used herein, and in the absence of a spécifie reference to a particular pharmaceutically acceptable sait and/or solvaté of emtricitabine, any dosages, whether expressed in e.g. milligrams or as a % by weight, should be taken as referring to the amount of emtricitabine, i.e. the amount of:

|0051] For example, therefore, a reference to “200 mg emtricitabine or a pharmaceutically acceptable sait and/or solvaté thereof’ means an amount of emtricitabine or a pharmaceutically acceptable sait and/or solvaté thereof which provides the same amount of emtncitabine as 200 mg of emtricitabine free base.

[0052] The amount of emtricitabine in a solid oral dosage form provided herein is generally between 180 mg and 220 mg, for instance between 190 mg and 210 mg, and more typically between 195 mg and 205 mg. In certain spécifie embodiments, solid oral dosage forms containing 200 mg of emtricitabine are provided.

Solid oral dosage forms

[0053] The inventors hâve successfully formulated the compound of Formula I, emtricitabine and tenofovir alafenamide in a single, stable dosage form that is pharmacologically efificacious and physically acceptable. The solid oral dosage forms disclosed herein are intended for pharmaceutical use in human subjects. Accordingly, they must be of an appropriate size and weight for oral human administration (e.g. they should hâve a total weight of less than about 1.5 g, e.g., less than about 1.0 g), in addition to being therapeutically efficacious.

[0054] In certain embodiments, formulations of the three active ingrédients into a solid oral dosage form which has a total weight of less than about 1.0 g are provided, for instance less than about 800 mg, or even less than about 750 mg, or even less than 700 mg. This is advantageous given that TRIUMEQ® (abacavir sulfate équivalent to 600 mg of abacavir, dolutegravir sodium équivalent to 50 mg of dolutegravir, and 300 mg of lamivudine) has a total weight of more than about 1000 mg, based on the weight of the active ingrédients in each tablet (due to the amount of excipients that are required to produce a pharmaceutically acceptable tablet). The provision of a relatively small dosage form (in particular a tablet) represents a clinical advantage because it may be expected to increase patient convenience and thus compliance as compared to larger dosage forms which are more burdensome for patients to swallow. In spécifie embodiments, the solid oral dosage form disclosed herein has a total weight of between 700 and 750 mg. In certain embodiments, the solid oral dosage form disclosed herein has a total weight of between 700 and 725 mg, or about 700 mg. In spécifie embodiments, the solid oral dosage form disclosed herein has a total weight of between about 50 and about 750 mg, between about 100 and about 750 mg, between about 200 and about 750 mg, or between about 250 and about 750 mg. The presently disclosed dosage forms may comprise less than 600 mg of excipients, such as less than 500 mg of excipients, or less than 450 mg of excipients. For example, solid oral dosage forms disclosed herein may comprise between 300 and 600 mg of excipients, or between 350 mg and 500 mg of excipients, or between 400 mg and 500 mg of excipients. Most typically, solid oral dosage forms disclosed herein comprise between 425 mg and 450 mg of excipients. In such embodiments, the dosage forms comprise as active ingrédients (a) 50 mg ofthe compound of Formula I or a pharmaceutically acceptable sait thereof, (b) 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) 200 mg emtricitabine or a pharmaceutically acceptable sait thereof. In certain embodiments, the dosage forms comprise as active ingrédients (a) 52 mg ofthe compound of Formula II, (b) 28 mg tenofovir alafenamide hemifumarate, and (c) 200 mg emtricitabine. In some embodiments, the dosage forms comprise as active ingrédients (a) 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) 200 mg emtricitabine or a pharmaceutically acceptable sait thereof. In certain embodiments, the dosage forms comprise as active ingrédients (a) 78 mg of the compound of Formula Π, (b) 28 mg tenofovir alafenamide hemifumarate, and (c) 200 mg emtricitabine.

[0055] The solid oral dosage forms disclosed herein will typically be in the form of a fixed dose combination tablet. This is because the inventors hâve found that the use of fixed dose combination tablets may assist in optimizing the pharmacokinetic properties of the active ingrédients, particularly the total exposure of the compound of Formula I or a pharmaceutically acceptable sait thereof, as measured by area under the curve (AUC) and Cma_X. In particular embodiments, the solid oral dosage forms disclosed herein are in the form of a multiiayer tablet. In certain embodiments, the use of a fixed dose combinations, e.g., multiiayer tablets, may affect the dissolution profile of one or more of the active ingrédients within the dosage form, and is therefore likely to hâve an impact on the in vivo pharmacokinetics of the dosage form. In particular, it has been observed that the dissolution of the compound of Formula I (e.g., as Formula II) varies depending on whether the tablet is in a fixed dose combination formulation with tenofovir alafenamide and emtricitabine and/or whether the tablet is a monolayer or multiiayer tablet. It has also been observed that the presence of certain excipients in the multiiayer tablet formulation (or absence of others) affects the dissolution profile of one or more of the active ingrédients within the dosage form. The provision of a tablet with particular pharmacokinetic parameters, e.g. pharmacokinetic parameters is a particular advantage afforded by the present disclosure.

|0056] In one embodiment, a multiiayer tablet comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) emtricitabine or a pharmaceutically acceptable sait thereof is provided. Typically, each layer contains at least one of (a), (b), and (c). For instance, in certain embodiments, the tablet comprises a first layer comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof, and a second layer comprising (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and further comprises (c) emtricitabine or a pharmaceutically acceptable sait thereof. In such embodiments, typically the first layer is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and/or the second layer is substantially free ofthe compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment the first layer is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof (e.g. the first layer contains less than l% by weight tenofovir alafenamide or a pharmaceutically acceptable sait 11 thereof), and the second layer is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof).

[0057] A particular embodiment provides a tablet, wherein the first layer comprises the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., Formula II) and is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than l% by weight tenofovir alafenamide or a pharmaceutically acceptable sait thereof), and the second layer comprises tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof). In a particular embodiment, a tablet is provided, wherein the first layer comprises 52 mg of the compound of Formula II and is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than l% by weight tenofovir alafenamide or a pharmaceutically acceptable sait thereof), and the second layer comprises 28 mg tenofovir alafenamide hemifumarate and 200 mg emtricitabine and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., the second layer contains less than 1% by weight ofthe compound of Formula I or a pharmaceutically acceptable sait thereof), wherein the first layer has a total weight of less than about 400 mg, such as about 325 mg, and the second layer has a total weight of less than about 450 mg, such as about 380 mg. In one embodiment, the layer containing tenofovir alafenamide or a pharmaceutically acceptable sait thereof does not contain lactose and/or starch. In one embodiment, the layer containing the compound of Formula I or a pharmaceutically acceptable sait thereof does not contain lactose, crospovidone and/or sodium stearyl fumarate.

[00581 A particular embodiment provides a tablet, wherein the first layer comprises the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., Formula Π) and is substantially free of emtricitabine or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than 1% by weight emtricitabine or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. the second layer contains less than 1% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof). In a particular embodiment, a tablet is provided, wherein (a) the first layer comprises 52 mg of the compound of Formula Π and is substantially free of emtricitabine or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than 1% by weight emtricitabine or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises 28 mg tenofovir alafenamide hemifumarate and 200 mg emtricitabine and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., 12 the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof), wherein the first layer has a total weight of less than about 400 mg, such as about 325 mg, and the second layer has a total weight of less than about 450 mg, such as about 380 mg. In one embodiment, the layer containing emtricitabine or a pharmaceutically acceptable sait thereof does not contain lactose and/or starch. In one embodiment, the layer containing the compound of Formula I or a pharmaceutically acceptable sait thereof does not contain lactose, crospovidone and/or sodium stearyl fumarate.

[0059J A particular embodiment provides a tablet, wherein the first layer comprises the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., Formula Π) and is substantially free of tenofovir alafenamide and emtricitabine or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than l% by weight each of tenofovir alafenamide and emtricitabine or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereofand is substantially free ofthe compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof). In a particular embodiment, a tablet is provided, wherein (a) the first layer comprises 52 mg of the compound of Formula II and is substantially free of tenofovir alafenamide and emtricitabine or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than l% by weight tenofovir alafenamide and emtricitabine or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises 28 mg tenofovir alafenamide hemifumarate and 200 mg emtricitabine and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof), wherein the first layer has a total weight of less than about 400 mg, such as about 325 mg, and the second layer has a total weight of less than about 450 mg, such as about 380 mg. In one embodiment, the layer containing tenofovir alafenamide and emtricitabine or a pharmaceutically acceptable sait thereof does not contain lactose and/or starch. In one embodiment, the layer containing the compound of Formula I or a pharmaceutically acceptable sait thereof does not contain lactose, crospovidone and/or sodium stearyl fumarate.

[0060] In a particular embodiment, a tablet is provided, wherein (a) the first layer comprises 78 mg of the compound of Formula Π and is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof (e.g. the first layer contains less than l% by weight tenofovir alafenamide or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises 28 mg tenofovir alafenamide hemifumarate and 200 mg emtricitabine and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. the second layer contains less than l% by weight of the compound of Formula I or a pharmaceutically acceptable sait thereof), wherein the first layer has a total weight of less than about 400 mg, such as about 355 mg, and the second layer has a 13 total weight of less than about 450 mg, such as about 380 mg. In one embodiment, the layer containing tenofovir alafenamide or a pharmaceutically acceptable sait thereof does not contain lactose and/or starch.

[0061] Unless otherwise specified, the terms “first layer”, “second layer”, “third layer” and so forth do not specify a particular order or orientation of the multilayer tablet formulations disclosed herein. Rather, these terms are used to distinguish the sections of the composition from each other and to specify the characteristics or components of each section or compartment. By way of example, in an embodiment, a tablet is provided wherein a first layer comprises 52 mg of the compound of Formula II and is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof (e.g., the first layer contains less than 1% by weight tenofovir alafenamide or a pharmaceutically acceptable sait thereof), and (b) the second layer comprises 28 mg tenofovir alafenamide hemifumarate and 200 mg emtricitabine and is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g., the second layer contains less than 1% by weight ofthe compound of Formula I or a pharmaceutically acceptable sait thereof), wherein the first layer has a total weight of less than about 400 mg, such as about 325 mg, and the second layer has a total weight of less than about 450 mg, such as about 380 mg. The first layer may be synthesized first or may be synthesized second. The first layer may be on the bottom or may be on the top or may be on a side. The term “first layer” is not limiting as to order and orientation.

[0062] The tablets disclosed herein are typically immédiate release tablets. In one embodiment, a tablet is provided which releases at least 50% of the compound of Formula I or a pharmaceutically acceptable sait thereof in about 20 minutes, measured using USP apparatus II, in 333 mL of fasted state simuiated intestinal fluid, pH 6.5, at 37 °C and paddle speed of 100 rpm. In certain embodiments, the tablets disclosed herein release at least 60% of the compound of Formula I or a pharmaceutically acceptable sait thereof in 20 minutes, measured using USP apparatus II, in 333 mL of 50 mM fasted state simuiated intestinal fluid, at 37 °C and paddle speed of 100 rpm. In some embodiments, a tablet that releases at least 70% of the compound of Formula I in 60 minutes is provided, measured using USP Apparatus Π, in 333 mL of fasted state simuiated intestinal fluid at 37 °C and paddle speed of 100 rpm.

[0063] Tablets disclosed herein will generally hâve a hardness within the range 14-20 kP, and, in certain spécifie embodiments, hâve a hardness of 17 kP. Hardness can conveniently be assessed by driving an anvil to compress a tablet at a constant loading rate until it fractures, operating in accordance with USP < 1217> (using e.g. a TBH 220, ERWEKA GmbH, Heusenstamm Germany hardness tester).

[0064J Tablets disclosed herein will generally hâve a friability of <1% by weight. Friability can be assessed according to USP < 1216>.

|0065J The core of a tablet provided herein may hâve a hardness of between 14-20 kP, and a friability of <l% by weight.

[0066} Tablets will typically include one or more excipients. Excipients should be compatible with the other ingrédients of the formulation and physiologically innocuous to the récipient thereof. Examples of suitable excipients are well known to the person skilled in the art of tablet formulation and may be found e.g. in Handbook of Pharmaceutîcal Excipients (eds. Rowe, Sheskey & Quinn), 6th édition 2009. As used herein the term “excipients” is intended to refer to inter alia basifying agents, solubilisera, glidants, fillers, binders, lubricant, diluents, preservatives, surface active agents, dispersing agents and the like. The term also includes agents such as sweetening agents, flavoring agents, coloring agents, preserving agents, and coating agents. Such components will generally be present in admixture within the tablet.

[0067] Examples of solubilisera include, but are not limited to, ionic surfactants (including both ionic and non-ionic surfactants) such as sodium lauryl sulphate, cetyltrimethylammonium bromide, polysorbates (such as polysorbate 20 or 80), poloxamers (such as poloxamer 188 or 207), and macrogols. In a particular embodiment, a tablet that comprises the compound of Formula I or a pharmaceutically acceptable sait thereof, includes a polysorbate, in particular polysorbate 20. In certain spécifie embodiments, the amount of polysorbate 20 in a tablet disclosed herein is less than about 5 mg, such as less than about 1 mg, or about 0.5 mg.

[0068[ Examples of lubricants, glidants and flow aids include, but are not limited to, magnésium stéarate, calcium stéarate, stearic acid, hydrogenated vegetable oil, glyceryl palmitostearate, glyceryl behenate, sodium stearyl fumarate, colloïdal Silicon dioxide, and talc. The amount of lubricant in a tablet is generally between about 0.5-5% by weight. In certain embodiments, the amount of lubricant in a tablet is about 1.5% by weight. In certain spécifie embodiments, tablets disclosed herein include magnésium stéarate. In certain other embodiments, the tablets disclosed herein do not include sodium stearyl fumarate. In certain embodiments, the tablet includes less than about 10 mg magnésium stéarate, or less than about 7.5 mg magnésium stéarate. In certain embodiments, the tablet includes less than about 9 mg magnésium stéarate, or less than about 8.75 mg magnésium stéarate. In certain embodiments, the tablet includes about 5 mg to about 10 mg magnésium stéarate, or about 6 mg to about 9 mg magnésium stéarate, or about 7 mg to about 9 mg magnésium stéarate, or about 8 mg to about 9 mg magnésium stéarate, or about 8.1 mg, about 8.2 mg, about 8.3 mg, about 8.4 mg, about

8.5 mg, about 8.6 mg, about 8.7 mg, about 8.8 mg or about 8.9 mg magnésium stéarate.

[0069] Examples of disintegrants include, but are not limited to, starches, celluloses, cross-linked PVP (crospovidone), sodium starch glycolate, croscarmellose sodium, etc. In certain embodiments, the tablets disclosed herein include croscarmellose sodium. In certain other embodiments, the tablets disclosed herein do not include crospovidone. In certain embodiments, the tablet includes less than about 50 mg croscarmellose sodium, or less than about 25 mg croscarmellose sodium. In certain embodiments, the tablet includes about 30 mg to about 60 mg croscarmellose sodium, or about 40 mg to about 60 mg croscarmellose sodium, or about 45 mg to about 55 mg croscarmellose sodium, or about 45 mg, about 46 mg, about 47 mg, about 48 mg, about 49 mg, about 50 mg, about 51 mg, about 52 mg or about 53 mg, or about 54 mg, or about 55 mg croscarmellose sodium.

[0070] Examples of fîllers (also known as bulking agents or diluents) include, but are not limited to, starches, maltodextrins, polyols (such as lactose), and celluloses. In certain embodiments, tablets provided herein may microcrystalline cellulose. In certain other embodiments, tablets provided herein do not contain lactose. In certain embodiments, tablets provided herein include less than about 300 mg mîcrocrystalline cellulose, in particular less than about 250 mg microcrystalline cellulose, and/or less than about 225 mg microcrystalline cellulose. In certain embodiments, tablets provided herein include less than about 500 mg microcrystalline cellulose, or less than about 450 mg microcrystalline cellulose, or less than about 400 mg mîcrocrystalline cellulose, or less than about 375 mg microcrystalline cellulose. In certain embodiments, tablets provided herein include about 250 mg to about 500 mg mîcrocrystalline cellulose, or about 300 mg to about 450 mg mîcrocrystalline cellulose, or about 300 mg to about 400 mg mîcrocrystalline cellulose, or about 325 mg to about 375 mg microcrystalline cellulose, or about 350 mg to about 370 mg mîcrocrystalline cellulose. In certain embodiments, tablets provided herein include about 300 mg, or about 310 mg, or about 320 mg, or about 330 mg, or about 340 mg, or about 350 mg, or about 360 mg, or about 370 mg, or about 380 mg, or about 390 mg, or about 400 mg microcrystalline cellulose.

[00711 Examples of binders include, but are not limited to, cross-linked PVP, HPMC, sucrose, starches, etc.

|0072] In certain embodiments, tablets provided herein are uncoated. In certain other embodiments, tablets provided herein are coated (in which case they include a coating). Although uncoated tablets may be used, it is more usual in the clinical setting to provide a coated tablet, in which case a conventional non-enteric coating may be used. Film coatings are known in the art and can be composed of hydrophilic polymer materials, but are not limited to, polysaccharide materials, such as hydroxypropylmethyl cellulose (HPMC), methylcellulose, hydroxyethyl cellulose (HEC), hydroxypropyl cellulose (HPC), poly(vinylalcohol-co-ethylene glycol) and other water soluble polymers. Though in certain embodiments the water soluble material included in the film coating of the embodiments disclosed herein includes a single polymer material, in certain other embodiments it is formed using a mixture of more than one polymer. In certain embodiments, the coating is yellow or brown. Suitable coatings include, but are not limited to, polymeric film coatings such as those comprising polyvinyl alcohol e.g. Opadry® IT (which includes part-hydrolysed PVA, titanium dioxide, macrogol 3350 (PEG) and talc, with optional colouring such as iron oxide (e.g., iron oxide red or iron oxide black) or indigo carminé or iron oxide yellow or FD&C yellow #6). The amount of coating is generally between about 2-4% of the core’s weight, and in certain spécifie embodiments, about 3%. Unless specifically stated otherwise, where the dosage form is coated, it is to be understood that a reference to % weight of the tablet means that of the total tablet, i.e. including the coating.

Pharmacokinetics

[0073| In certain embodiments, the pharmaceutical compositions disclosed herein resuit in increased systemic exposure (AUCmf, Cnu_X) for the compound of Formula I or a pharmaceutically acceptable sait thereof. In particular embodiments, the multilayer tablet formulations disclosed herein resuit in increased systemic exposure for the compound of Formula I or a pharmaceutically acceptable sait thereof compared to a single agent tablet formulation of the compound of Formula I or a pharmaceutically acceptable sait thereof. In certain embodiments, the multilayer tablet formulation results in an increase of at least about 20% in the systemic exposure of the compound of Formula 1 or a pharmaceutically acceptable sait thereof compared to a single agent tablet formulation of the compound of Formula I or a pharmaceutically acceptable sait thereof. In some embodiments, the increase in systemic exposure is at least about 25% or at least about 30%. In some embodiments, the increase in systemic exposure is about 30%.

r .S=jnax.

[0074] Cnux is the maximum observed plasma/serum concentration of drug.

[0075] In particular embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma C_ŒX of the compound of Formula I in fasted patients of from about 5300 to about 8900 ng/mL, e.g. about 7100 ng/mL.

[0076| In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cn^ of emtricitabine in fasted patients of from about 1700 to about 2800 ng/mL, e.g. about 2300 ng/mL.

[00771 In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cmax of tenofovir alafenamide in fasted patients of from about 190 to about 320 ng/mL, e.g. about 250 ng/mL.

[0078] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cnux of the Compound of Formula I of from about from about 4200 ng/mL to about 8000 ng/mL, regardless of whether the subject was fed or fasted.

[0079] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma of the compound of Formula I in fasted patients of from about 4200 ng/mL to about 6500 ng/mL, or from about 4700 ng/mL to about 5300 ng/mL, or from about 4700 ng/mL to about 5800 ng/mL, or from about 5000 ng/mL to about 5500 ng/mL.

[0080] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cmax of the compound of Formula I in fed patients of from about 4500 ng/mL to about 8000 ng/mL, or from about 4800 ng/mL to about 7900 ng/mL, or from about 5300 ng/mL to about 6900 ng/mL, or from about 5600 ng/mL to about 6600 ng/mL.

[0081] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma C™* of emtricitabine of from about from about 1770 ng/mL to about 2800 ng/mL, regardless of whether the subject was fed or fasted.

[0082| In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma of emtricitabine in fasted patients of from about 1770 ng/mL to about 2800 ng/mL, or from about 2000 ng/mL to about 2600 ng/mL, or from about 2000 ng/mL to about 2500 ng/mL, or from about 2100 ng/mL to about 2400 ng/mL.

|0083] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cmax of emtricitabine in fed patients of from 18 about 1000 ng/mL to about 3000 ng/mL, or from about 1500 ng/mL to about 2000 ng/m from about 1700 ng/mL to about 2200 ng/mL L, or from about 1800 ng/mL to about 2100 ng/mL.

[0084] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cm^ of tenofovir alafenamide of from about from about 185 ng/mL to about 315 ng/mL, regardless of whether the subject was fed or fasted.

[0085] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma of tenofovir alafenamide in fasted patients of from about 185 ng/mL to about 315 ng/mL, or from about 200 ng/mL to about 300 ng/mL, or from about 210 ng/mL to about 290 ng/mL, or from about 220 ng/mL to about 275 ng/mL, or from about 230 ng/mL to about 265 ng/mL, or from about 240 ng/mL to about 260 ng/mL.

[0086] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma Cnux of tenofovir alafenamide in fed patients offrom about 150 ng/mL to about 350 ng/mL, or from about 185 ng/mL to about 300 ng/m from about 210 ng/mL to about 280 ng/mL L, or from about 250 ng/mL to about 265 ng/mL.

AUÇinf

[0087| AUCinf is the area under the plasma/serum concentration versus time curve extrapolated to infinité time, calculated as AUCo-iasi+ (Chst/Az)

[00881 In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of the compound of Formula I in fed patients of from about 117000 to about 196000 h*ng/mL, e.g. about 157000 h»ng/mL.

[0089] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of emtricitabine in fed patients of fforn about 8700 to about 14500h*ng/mL, e.g. about 2300h»ng/mL.

[0090] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of tenofovir alafenamide in fed patients of from about 150 and 260 h*ng/mL, e.g. about 210 h*ng/mL.

[0091] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of the Compound of Formula I of from about from about 84450 h*ng/mL to about 141000 h«ng/mL, regardless of whether the subject was fed or fasted.

[0092] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of the compound of Formula I in fasted patients of from about 84450 h*ng/mL to about 141000 h*ng/mL, or from about 90000 h«ng/mL to about 135000 h*ng/mL, or from about 95000 h*ng/mL to about 130000 h«ng/mL, or from about 100000 h«ng/mL to about 125000 h*ng/mL, or from about 110000 h*ng/mL to about 120000 h*ng/mL.

[0093] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of the compound of Formula I in fed patients of from about 100000 hmg/mL to about 200000 ng/mL, or from about 112000 h»ng/mL to about 175000 ng/mL, or from about 126000 h«ng/mL to about 155000 ng/mL, or from about 133000 h«ng/mL to about 147000 ng/mL.

[0094] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of emtricitabine of from about from about 8100 h»ng/mL to about 13600 h-ng/mL, regardless of whether the subject was fed or fasted.

[0095] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of emtricitabine in fasted patients of from about 8100 h*ng/mL to about 13600 h*ng/mL, or from about 8700h*ng/mL to about 13000 20 h*ng/mL, or from about 92000 h*ng/mL to about 12500 h’ng/mL, or from about 9700 h*ng/mL to about 12000 h*ng/mL, or from about 10000 h*ng/mL to about 11400 h«ng/mL.

[0096] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUC_inf of emtricitabine in fed patients of from about 7500 h*ng/mL to about 15000 ng/mL, or from about 8300 h*ng/mL to about 14000 ng/mL, or from about 9500 h*ng/mL to about 12000 ng/mL, or from about 9900 h»ng/mL to about 11600 ng/mL.

[0097] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of tenofovir alafenamide of from about from about 200 h*ng/mL to about 500 h*ng/niL, regardless of whether the subject was fed or fasted.

[0098] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of tenofovir alafenamide in fasted patients of from about 200 h*ng/mï. to about 265 h*ng/mL, or from about 200 h*ng/mL to about 300 h«ng/mL, or from about 210 h*ng/mL to about 290 h*ng/mL, or from about 220h»ng/mL to about 270 h*ng/mL, or from about 230 h· ng/mL to about 265 h*ng/mL.

[0099] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCinf of tenofovir alafenamide in fed patients of from about 200 h’ng/mL to about 500 ng/mL, or from about 230 h’ng/mL to about 400 ng/mL, or from about 260 h’ng/mL to about 350 ng/mL, or from about 275 h’ng/mL to about 370 ng/mL.

AUCh;,

[0100] AUCi_ast is the area under the plasma/serum concentration versus time curve from time zéro to the last quantifiable concentration.

[01011 In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCj_iSl of the compound of Formula I in fed patients of from about 114000 to about 190000 h’ng/mL, e.g. about 152000 h’ng/mL.

(0102] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCi_m1 of emtricitabine in fed patients of from about 8600 to about 14000 h’ng/mL, e.g. about 11000 h*ng/mL.

|0103] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 75 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChst of tenofovir alafenamide in fed patients of from about 150 and 260 h’ng/mL, e.g. about 210 h’ng/mL.

[01041 In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChst of the compound of Formula I of from about from about 81700 h’ng/mL to about 140000 h’ng/mL, regardless of whether the subject was fed or fasted.

[0105] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCi_ast of the compound of Formula I in fasted patients of from about 81700 h’ng/mL to about 140000 h’ng/mL, from about 87000 h’ng/mL to about 131000 h’ng/mL, from about 92000 h*ng/mL to about 130000 h*ng/mL, from about 98100 h’ng/mL to about 120000 h’ng/mL, from about 104000 h’ng/mL to about 115000 h’ng/mL.

[0106] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChst of the compound of Formula I in fed patients of from about 100000 h’ng/mL to about 200000 ng/mL, or from about 108000 h’ng/mL to about 170000 ng/mL, or from about 122000 h’ng/mL to about 150000 ng/mL, or from about 128000 h’ng/mL to about 142000 ng/mL.

[0107] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChsi of emtricitabine of from about from about 7500 h»ng/mL to about 15000 h· ng/mL, regardless of whether the subject was fed or fasted.

[0108] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChst of the emtricitabine in fasted patients of from about 8000 h*ng/mL to about 13400 h*ng/mL, from about 8500 h’ng/mL to about 12800 h«ng/mL, from about 9000 h«ng/mL to about 12300 h· ng/mL, from about 9500h*ng/mL to about 11000 h· ng/mL, from about 10000 h«ng/mL to about 11200 h*ng/mL.

[0109] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUC'i,_sl of emtricitabine in fed patients of from about 7500 h*ng/mL to about 15000 ng/mL, or from about 8000 h*ng/mL to about 14000 ng/mL, or from about 9000 h«ng/mL to about 12000 ng/mL, or from about 9700 h*ng/mL to about 11300 ng/mL.

[0110] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCt_ast of tenofovir alafenamide of from about from about 165 h’ng/mL to about 400 h»ng/mL, regardless of whether the subject was fed or fasted.

[0111] In certain spécifie embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUChst of the tenofovir alafenamide in fasted patients of from about 165 h«ng/mL to about 390 h»ng/mL, from about 186 h*ng/mL to about 227 h*ng/mL, from about 196 h»ng/mL to about 217 h»ng/mL.

[0112] In particular embodiments, a pharmaceutical composition comprising a tablet containing 50 mg of the compound of Formula I or a pharmaceutically acceptable sait thereof, 200 mg emtricitabine or a pharmaceutically acceptable sait thereof, and 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof provide a plasma AUCiasi of tenofovir alafenamide in fed 23 patients of from about 200 h*ng/mL to about 400 ng/mL, or from about 230 h*ng/mL to about 390 ng/mL, or from about 260 h*ng/mL to about 345 ng/mL, or from about 275 h*ng/mL to about 330 ng/mL.

Ctasï

[01131 C_Iaslis the last observed quantifiable plasma/serum concentration of the drug.

[0114] Cnux, C_bst, AUCinf, and AUCu_sl are standard pharmacokinetic parameters that can be estimated manually or by using modelling software well known in the art, such as the Pharsight WinNonlin package using a non-compartmental model. The general basis for calculation of these quantities is well-known (e.g. see Rowland & Tozer (2010) Clinical Pharmacokinetics and Pharmacodynamies: Concepts and Applications ISBN 978-0781750097, or Jambhekar & Breen (2012) Basic Pharmacokinetics ISBN 978-0853699804). Typically the parameters will be assessed as the average (e.g. géométrie or arithmetic mean) from within a group of at least 12 (and normally between 24 and 36) healthy human adults. Parameters should be measured in accordance with standards and practices which would be acceptable to a pharmaceutical regulatory agency such as FDA, EMA, MHLW, or WHO. The values may be based on measurements taken at appropriate intervals following the time of tablet ingestion, such as every hour, or at increasingly sparse sampling intervals, such as 1, 3, 5, 7, 9, 11, 13, 15, 20, and 24 hours after ingestion. They can be assessed either following a single-dose of drug or at steady State, but will typically be assessed following a single-dose.

|0115] It is well known in the bioavailability and bioequivalence arts how to détermine whether any particular tablet meets regulatory requirements for équivalent bioavailability and pharmacokinetic bioequivalence e.g. see: Niazi (2014) Handbook of Bioequivalence Testing, 2nd Edition, ISBN 9781482226379; Guidance for Industry Bioavailability and Bioequivalence Studies for Orally Administered Drug Products — General Considérations FDA March 2003; and Guideline On The Investigation Of Bioequivalence, EMEA 2010 CPMP/EWP/QWP/1401/98 Rev. 1/ Coït **. To ensure statistical power a study to measure the Cm^, AUCi_ast and AUCinf values will be performed in multiple subjects e.g. in a group of at least 12 (and normally between 24 and 36) healthy human adults.

[0116] Because determining the C_m4X, AUCi_ast and AUCinf values is necessarily destructive these parameters will not be determined directly for the dosage form (in particular the tablet) in question, but rather for a dosage form made by the same manufacturing process with the same components. Thus a batch of a dosage form (e.g. tablets) can be made by a particular process, and the 90% confidence interval of C_m3X, AUCi_Ml and AUCinf will be assessed on a sample of those tablets. If these values meet the 80-125% requirement noted above then tablets made by the manufacturing process in question are tablets ofthe present invention.

[0117] A fixed dose combination tablet is provided comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. Formula Π), (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) emtricitabine or a pharmaceutically acceptable sait thereof.

[0118| In an embodiment a multilayer tablet is provided, comprising (a) the compound of Formula I or a pharmaceutically acceptable sait thereof (e.g. Formula Π), (b) tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) emtricitabine or a pharmaceutically acceptable sait thereof.

[0119] In an embodiment, the multilayer tablet disclosed herein comprises (a) a first layer comprising the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) a second layer containing tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (c) further comprises emtricitabine or a pharmaceutically acceptable sait thereof.

[0120] In an embodiment of the multilayer tablet disclosed herein, (a) the first layer is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and/or (b) the second layer is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof.

[01211 In an embodiment of the multilayer tablet disclosed herein, (a) the first layer is substantially free of emtricitabine or a pharmaceutically acceptable sait thereof, and/or (b) the second layer is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0122] In an embodiment of the multilayer tablet disclosed herein, (a) the first layer is substantially free of emtricitabine or a pharmaceutically acceptable sait thereof and tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and/or (b) the second layer is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0123] In an embodiment, the multilayer tablet disclosed herein comprises (a) a first layer comprising the compound of Formula I or a pharmaceutically acceptable sait thereof, (b) a second layer containing emtricitabine or a pharmaceutically acceptable sait thereof, and (c) further comprises tenofovir alafenamide or a pharmaceutically acceptable sait thereof.

[0124] In an embodiment of the multilayer tablet disclosed herein, (a) the first layer is substantially free of emtricitabine or a pharmaceutically acceptable sait thereof, and/or (b) the second layer is substantially free of the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0125| In an embodiment of the multilayer tablet disclosed herein, (a) the first layer comprises the compound of Formula I or a pharmaceutically acceptable sait thereof and is substantially free of tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and (b) the second layer comprises tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof and is substantially firee of the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0126| In an embodiment of the muitilayer tablet disclosed herein, the first layer is substantially free of emtricitabine.

[0127| In one embodiment, the muitilayer tablet disclosed herein comprises 50 ±6 mg of the compound of Formula I. In one embodiment, the muitilayer tablet disclosed herein comprises 200 ± 20 mg of emtricitabine. In one embodiment, the muitilayer tablet disclosed herein comprises 25 ± 3 mg of tenofovir alafenamide.

(0128| In one embodiment, the muitilayer tablet disclosed herein comprises 75 ± 6 mg of the compound of Formula I. In one embodiment, the muitilayer tablet disclosed herein comprises 200 ± 20 mg of emtricitabine. In one embodiment, the muitilayer tablet disclosed herein comprises 25 ± 3 mg of tenofovir alafenamide.

[0129] In one embodiment, the muitilayer tablet disclosed herein comprises 52 ± 6 mg of the compound of Formula H. In one embodiment, the muitilayer tablet disclosed herein comprises 200 ± 20 mg of emtricitabine. In one embodiment, the muitilayer tablet disclosed herein comprises 28 ± 3 mg of tenofovir alafenamide hemifumarate.

|0130] In one embodiment, the muitilayer tablet disclosed herein comprises 78 ± 6 mg of the compound of Formula II. In one embodiment, the muitilayer tablet disclosed herein comprises 200 ± 20 mg of emtricitabine. In one embodiment, the muitilayer tablet disclosed herein comprises 28 ± 3 mg of tenofovir alafenamide hemifumarate.

[0131 ] In one embodiment, a first layer of the muitilayer tablet disclosed herein comprises one or more excipients, for example one or more diiuents, disintegrants, binders, or lubricants.

[0132] In one embodiment, a first layer of the muitilayer tablet comprises croscarmellose sodium. In one embodiment, a first layer of the muitilayer tablet comprises croscarmellose sodium, microcrystalline cellulose, and magnésium stéarate.

[0133] In one embodiment a tablet is provided wherein less than about 25 weight percent of a first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein less than about 20 weight percent of a first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein less than about 16 weight percent of a first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein about 5 to about 20 weight percent, or about 10 to about 18 weight percent, or about 14 to about 18 weight percent of a first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein about 16 weight percent of a first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0134] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 290 mg.

[0135j In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula Π and wherein the total weight of the first layer is at least about 300 mg.

[0136] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 310 mg.

[0137] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg ofthe compound ofFormula Π and wherein the total weight ofthe first layer is at least about 320 mg.

[0138] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula Π and wherein the total weight of the first layer is at least about 330 mg.

[0139] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 340 mg.

[0140] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula Π and wherein the total weight of the first layer is at least about 350 mg.

[0141] In one embodiment a tablet is provided wherein the first layer comprises 52 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 360 mg.

[0142] In one embodiment a tablet is provided wherein the first layer comprises 52±2.8 mg of the compound of Formula Π and wherein the total weight ofthe first layer is at least about 290 mg and is less than about 360 mg.

[0143] In one embodiment a tablet is provided wherein the first layer comprises 52 ±2.8 mg of the compound of Formula Π and wherein the total weight of the first layer is at least about 300 mg and is less than about 350 mg.

[0144] In one embodiment a tablet is provided wherein the first layer comprises 52 ±2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 310 mg and is less than about 330 mg.

[0145] In one embodiment, the first layer of the multilayer tablet has a total weight of 323 ± 75 mg, or 323 ± 25 mg, or 323 ± 10 mg, or 323 mg.

[0146| In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient

Mass (mg)

The compound of Formula I or a sait thereof	40-60
Microcrystalline cellulose	200-400
Croscarmellose sodium	1-40
Magnésium stéarate	1-10

[0147] In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
the compound of Formula II	30-60
Microcrystalline cellulose	200-400
Croscarmellose sodium	1-40
Magnésium stéarate	2-8

|0148] In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
the compound of Formula II	50 ±6
Microcrystalline cellulose	250 ± 20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

]0149] In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
the compound of Formula II	52 ±5.6
Microcrystalline cellulose	246 ± 10
Croscarmellose sodium	19 ± 2.5
Magnésium stéarate	5 ±0.75

|0150] In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
the compound of Formula Π	52 ±2.8

Microcrystaliine cellulose	246 ±5
Croscarmellose sodium	19 ± 1.75
Magnésium stearate	5 ±0.5

[0151] In one embodiment, the first layer of the multiiayer tablet consists of:

Ingrédient	Mass (mg)
the compound of Formula II	52.5
Microcrystalline cellulose	246.3
Croscarmellose sodium	19.4
Magnésium stearate	4.9

[0152] In one embodiment, the first layer of the multiiayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
the compound of Formula I or a pharmaceutically acceptable sait thereof	52.5 ± 1.6
Microcrystalline cellulose	214 ±7.3
Croscarmellose sodium	19 ±0.6
Magnésium stearate	2.4 ±0.5
Extragranular
Microcrystalline cellulose	32 ± 1
Magnésium stearate	2.4 ±0.5
Total layer weight	323

[0153] In one embodiment, the first layer of the multiiayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
the compound of Formula Π	52.5 ±1.6
Microcrystalline cellulose	214 ± 7.3
Croscarmellose sodium	19 ±0.6
Magnésium stearate	2.4 ±0.5

Extragranular
Microcrystalline cellulose	32 ± l
Magnésium stearate	2.4 ±0.5
Total layer weight	323

[0154| In one embodiment, the first layer of the multilayer tablet consists of

Ingrédient	Mass (mg)
Intergranular
the compound of Formula II	52.5 ±1.6
Microcrystalline cellulose	214 ±6.4
Croscarmellose sodium	19.4 ±0.6
Magnésium stearate	2.45 ±0.5
Extragranular
Microcrystalline cellulose	32.3 ± 1
Magnésium stearate	2.45 ±0.5
Total layer weight	323

[0155| In one embodiment, the first layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
the compound of Formula I, or a pharmaceutically acceptable sait therof	52.5 ±1.6
Microcrystalline cellulose	214±6.4
Croscarmellose sodium	19.4 ±0.6
Magnésium stearate	2.43 ±0.5
Extragranular
Microcrystalline cellulose	32.3 ± 1
Magnésium stearate	2.43 ±0.5
Total layer weight	323

[0156] In one embodiment, the first layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
the compound of Formula II	52.5 ± 1.6
Microcrystalline cellulose	214 ±6.4
Croscarmellose sodium	19.4 ±0.6
Magnésium stéarate	2.43 ±0.5
Extragranular
Microcrystalline cellulose	32.3 ± 1
Magnésium stéarate	2.43 ±0.5
Total layer weight	323

[0157| In one embodiment a tablet is provided wherein less than about 30 weight percent of the first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein less than about 25 weight percent of the first layer is the compound of Formula 1 or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein less than about 22 weight percent of the first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein about 15 to about 27 weight percent, or about 17 to about 25 weight percent, or about 19 to about 23 weight percent of the first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof. In one embodiment a tablet is provided wherein about 21 weight percent of the first layer is the compound of Formula I or a pharmaceutically acceptable sait thereof.

[0158] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 320 mg.

[0159] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 330 mg.

[0160J In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 340 mg.

[0161] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 350 mg.

[0162J In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 360 mg.

[0163] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 370 mg.

[0164] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 380 mg.

[0165] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound ofFormula II and wherein the total weight ofthe first layer is at least about 390 mg.

(0166] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 320 mg and is less than about 390 mg.

[0167] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound ofFormula Π and wherein the total weight ofthe first layer is at least about 330 mg and is less than about 3 80 mg.

[0168] In one embodiment a tablet is provided wherein the first layer comprises 78 ± 2.8 mg of the compound of Formula II and wherein the total weight of the first layer is at least about 350 mg and is less than about 360 mg.

[0169] In one embodiment, the first layer of the multilayer tablet has a total weight of 353 ± 75 mg, or 353 ± 25 mg, or 353 ± 10 mg, or 353 mg.

|0170] In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
The compound of Formula I or a sait thereof	40-85
Microcrystalline cellulose	200-400
Croscarmellose sodium	1-40
Magnésium stéarate	1-10

[0171 j In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
The compound of Formula II	30-90
Microcrystalline cellulose	200-400
Croscarmellose sodium	1-40
Magnésium stéarate	2-8

[0172] In one embodiment, the first layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
The compound of Formula II	80 ±6
Microcrystalline cellulose	250 ± 20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

[0173] In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
The compound of Formula Π	78 ±5.6
Mîcrocrystalline cellulose	250± 10
Croscarmellose sodium	20±2.5
Magnésium stéarate	5 ±0.75

[0174] In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
the compound of Formula II	78 ±2.8
Mîcrocrystalline cellulose	247 ± 5
Croscarmellose sodium	21 ±1.75
Magnésium stéarate	5 ±0.5

[0175| In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
the compound of Formula Π	78.7
Microcrystalline cellulose	247.8
Croscarmellose sodium	21.2
Magnésium stéarate	5.3

[0176] In one embodiment, the first layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
Intergramilar
the compound of Formula II	78.7
Microcrystalline cellulose	212.5
Croscarmellose sodium	21.2
Magnésium stéarate	2.65
Extragranular
Microcrystalline cellulose	35.3
Magnésium stéarate	2.65
Total layer weight	353

[0177] In one embodiment, the second layer of the multilayer tablet does not comprise lactose. In one embodiment, the second layer of the multilayer tablet does not comprise starch. In one embodiment, the second layer of the multilayer tablet comprises neither lactose nor starch.

[0178] In one embodiment, the second layer of the multilayer tablet comprises one or more excipients, for example, one or more diluents, dis intégrants, binders, or lubricants.

[0179] In one embodiment, the second layer of the multilayer tablet comprises microcrystalline cellulose and croscarmellose sodium.

[0180] In one embodimenL the second layer of the multilayer tablet comprises microcrystalline cellulose, croscarmellose sodium and magnésium stéarate.

[0181] In one embodiment, the second layer of the multilayer tablet comprises 20-40 mg of croscarmellose sodium. In one embodiment, the second layer of the multilayer tablet comprises 105125 mg of microcrystalline cellulose. In one embodiment, the second layer of the multilayer tablet comprises 1-8 mg of magnésium stéarate.

[0182] In one embodiment, the second layer of the multilayer tablet does not comprise lactose. In one embodiment, the second layer of the multilayer tablet does not comprise starch. In one embodiment, the second layer ofthe multilayer tablet comprises neither lactose nor starch.

[0183] In one embodiment, the second layer of the multilayer tablet has a total weight of less than 600 mg, or less than 500 mg, or less than 400 mg, or less than 380 mg. In one embodiment, the second layer of the multilayer tablet has a total weight of 377 mg ± 50 mg or 377 mg ± 25 mg, or 377 mg ± 5 mg, or 377 mg.

[0184| In one embodiment, over 50 % by weight of the second layer of the multilayer tablet is emtricitabine or a sait thereof and tenofovir alafenamide or a sait thereof. In one embodiment, over 55 % by weight of the second layer of the multilayer tablet is emtricitabine or a sait thereof and tenofovir alafenamide or a sait thereof. In one embodiment, over 60 % by weight of the second layer of the multilayer tablet is emtricitabine or a sait thereof and tenofovir alafenamide or a sait thereof. In one embodiment, about 55 % to about 65% by weight of the second layer of the multilayer tablet is emtricitabine or a sait thereof and tenofovir alafenamide or a sait thereof. In one embodiment, over 60 % by weight of the second layer of the multilayer tablet is emtricitabine and tenofovir alafenamide hemifumarate. In one embodiment about 55 % to about 65% by weight of the second layer of the multilayer tablet is emtricitabine and tenofovir alafenamide hemifumarate.

[0185] In one embodiment, the second layer of the multilayer tablet contains less than 250 mg of excipients, for example less than 200 mg, or less than 150 mg.

[0186] In one embodiment, at least 50% by weight of the second layer of the multilayer tablet is emtricitabine. In one embodiment, at least 53% by weight of the second layer of the multilayer tablet is emtricitabine. In one embodiment, about 45% to about 65% by weight of the second layer of the multilayer tablet is emtricitabine. In one embodiment, about 50% to about 60% by weight of the second layer of the multilayer tablet is emtricitabine. In one embodiment, about 51% to about 53% by weight of the second layer of the multilayer tablet is emtricitabine.

[0187] In one embodiment, at least 4% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, at least 6% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, at least 7% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, about 5% to about 10% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, about 6% to about 9% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, about 7% to about 8% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate. In one embodiment, about 7% by weight of the second layer of the multilayer tablet is tenofovir alafenamide hemifumarate.

[0188| In one embodiment, less than 20% by weight of the second layer of the multilayer tablet is croscarmellose sodium. In one embodiment, less than 10% by weight of the second layer of the multilayer tablet is croscarmellose sodium. In one embodiment, about 5% to about 10% by weight of the second layer of the multilayer tablet is croscarmellose sodium. In certain embodiments, the use of about 5 to 7% (e.g. about 6%) croscarmellose sodium by weight of the second layer may provide enhanced dissolution of the compound of Formula I or a pharmaceutically acceptable sait thereof, relative to other disintegrants e.g. polyvinylpyrrolidone (crospovidone). In certain embodiments, the use of about 5 to 10% croscarmellose sodium by weight of the second layer may provide enhanced dissolution of the compound of Formula I or a pharmaceutically acceptable sait thereof, relative to other disintegrants e.g. polyvinylpyrrolidone (crospovidone).

[0189] In one embodiment, less than 50% by weight of the second layer of the multilayer tablet is microcrystalline cellulose. In one embodiment, less than 40% by weight of the second layer of the multilayer tablet is microcrystalline cellulose. In one embodiment, less than 31% by weight of the second layer of the multilayer tablet is microcrystalline cellulose.

[0190] In one embodiment, less than 5% by weight of the second layer of the multilayer tablet is magnésium stéarate. In one embodiment, less than 3% by weight of the second layer of the multilayer tablet is magnésium stéarate. In one embodiment, less than 2% by weight of the second layer of the multilayer tablet is magnésium stéarate. In one embodiment, about 0.5% to about 1.5% by weight of the second layer of the multilayer tablet is magnésium stéarate. In certain embodiments, the use of about 1% to about 2% (e.g. about 1.5%) magnésium stéarate by weight of the second layer may provide enhanced dissolution of the compound of Formula I or a pharmaceutically acceptable sait thereof, relative to other lubricants e.g. sodium stearyl fumarate.

[01911 In one embodiment, the total weight of the second layer is less than 150% of the total weight ofthe first layer. In one embodiment, the total weight ofthe second layer is less than 125% ofthe total weight ofthe first layer. In one embodiment, the total weight ofthe second layer is less than 116% of the total weight of the first layer. In one embodiment, the total weight of the second layer is less than 106% of the total weight of the first layer.

[0192] In one embodiment, the second layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
Emtricitabine or a sait thereof	150-250
Tenofovir alafenamide or a sait thereof	20-35
Microcrystalline cellulose	90-130
Croscarmellose sodium	20-35
Magnésium stéarate	1-7

[0193] In one embodiment, the second layer of the multilayer tablet comprises:

Ingrédient	Mass (mg)
Emtricitabine	170-230
Tenofovir alafenamide hemifumarate	22-32
Microcrystalline cellulose	100-120
Croscarmellose sodium	20-35
Magnésium stéarate	1-7

[0194] In one embodiment, the second layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	5.6 ±1.1

|0195] In one embodiment, the second layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28 ±1.4
Microcrystalline cellulose	113 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	5.6 ±0.5

[0196] In one embodiment, the second layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
Emtricitabine	200
Tenofovir alafenamide hemifumarate	28.1
Microcrystalline cellulose	113.2
Croscarmellose sodium	30.2
Magnésium stéarate	5.7

[0197] In one embodiment, the second layer of the multilayer tablet consists of:

Ingrédient	Mass (mg)
Intergranidar
Emtricitabine	200.00
Tenofovir alafenamide hemifumarate	28.1
Microcrystalline cellulose	113.2
Croscarmellose sodium	30.2
Magnésium stéarate	2.85
Extragranular
Magnésium stéarate	2.85
Total layer weight	377

[0198] In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide	25 ±3
Microcrystalline cellulose	115 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	3.8 ±1.1

[0199| In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	115 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	3.8 ±1.1

[0200] In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28 ±1.4
Microcrystalline cellulose	115 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	3.8 ±0.5

[0201| In one embodiment, the second layer ofthe multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200
Tenofovir alafenamide hemifnmarate	28.1
Microcrystalline cellulose	115
Croscarmellose sodium	30.2
Magnésium stéarate	3.8

|0202J In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
Emtricitabine	200
Tenofovir alafenamide hemifumarate	28
Microcrystalline cellulose	115
Croscarmellose sodium	30.2·
Magnésium stéarate	1.9
Extragranular
Magnésium stéarate	1.9
Total layer weight	377

[0203] In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200
Tenofovir alafenamide hemifumarate	28
Microcrystalline cellulose	H3.2
Croscarmellose sodium	30.2
Magnésium stearate	5.7

[0204| In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Emtricitabine	200
Tenofovir alafenamide hemifumarate	28
Microcrystalline cellulose	115
Croscarmellose sodium	30.2
Magnésium stearate	3.8

|0205] In one embodiment, the second layer of the multilayer tablet includes:

Ingrédient	Mass (mg)
Intergranular
Emtricitabine	200
Tenofovir alafenamide hemifumarate	28
Microcrystalline cellulose	113.2
Croscarmellose sodium	30.2
Magnésium stearate	2.85
Extragranular
Magnésium stearate	2.85
Total layer weight	377

[0206| In one embodiment of the multilayer tablet formulations disclosed herein, the first layer is in contact with the second layer.

[0207| In one embodiment, the first layer is produced first, followed be the second layer. That is, in one embodiment, the first layer is prepared and pressed into a first layer, followed by the second layer being prepared and being pressed with the first layer into a muitilayer tablet. In one embodiment, the second layer is produced first, followed be the first layer. That is, in one embodiment, the second layer is prepared and pressed into a second layer, followed by the first layer being prepared and being pressed with the second layer into a muitilayer tablet.

[0208] As used herein, when describing the muitilayer tablets disclosed herein, the terms “first layer” and “second layer” are not intended to indicate the method by which the tablets are produced, in particular the order in which the layers are obtained.

[0209] In certain embodiments, the muitilayer tablet further comprises additional layers. In certain embodiments, the additional layer or layers are located between the first and second layers. In certain embodiments, the additional layer or layers are located on either side of the first and/or second layer, such that they are an outside layer of the table and/or are disposed between the first and/or second layer and a coating layer. In some embodiments, the additional layer or layers encapsulate the first and second layers.

|0210] In one embodiment, the muitilayer tablet further comprises a third layer that is between and that séparâtes the first layer and the second layer. In one embodiment, the third layer of the muitilayer tablet comprises lactose monohydrate, or microcrystalline cellulose, or a mixture thereof.

{0211 ] In one embodiment, the muitilayer tablet further comprises a film coating. In one embodiment, the muitilayer tablet further comprises about 14 mg to about 28 mg of a film coating. In one embodiment, the muitilayer tablet further comprises about 19 mg to about 23 mg of a film coating. In one embodiment, the muitilayer tablet further comprises about 21 mg of a film coating.In one embodiment the film coating comprises polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide, and black iron oxide. In one embodiment the film coating consists of 21 ± 7 mg of Opadry Π Brown 85F165072. In one embodiment the film coating consists of 21 ± 7 mg of Opadry II Yellow 85F92259.

{0212] In one embodiment, the muitilayer tablet further includes a film coating. In one embodiment, the muitilayer tablet further comprises about 1.9% to about 3.9% w/w of a film coating. In one embodiment, the muitilayer tablet further comprises about 2.5% to about 3.5% w/w of a film coating. In one embodiment, the muitilayer tablet further comprises about 3% of a film coating. In one embodiment the film coating includes polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide, iron oxide red, and black iron oxide. In one embodiment the film coating includes 36%-40% polyvinyl alcohol, 18%-22% polyethylene glycol, 13%-16% talc, 20%-24% titanium dioxide, 2%-3% iron oxide red, and 0.5%-0.7% black iron oxide.

[0213] In one embodiment a tablet is provided comprising about 6.4-8.5% w/w of the compound of Formula Π, about 25-32% w/w mg emtricitabine, about 3.5-4.5% w/w mg tenofovir alafenamide hemifumarate, about 46-57% w/w mg microcrystalline cellulose, about 5 9-8.5% w/w mg croscarmellose sodium, and about 1.0-2.0% w/w mg magnésium stearate.

[0214] In one embodiment a tablet is provided comprising 52 ± 6 mg of the compound of Formula Π, 200 ± 20 mg emtricitabine, 28 ± 3 mg tenofovir alafenamide hemifumarate, 360 ± 30 mg Microcrystalline cellulose, 50 ±8 mg Croscarmellose sodium, and 10.5 ±3 mg magnésium stearate and wherein the total weight of the tablet is at least about 685 mg.

[0215] In one embodiment a tablet is provided comprising 52 ± 6 mg of the compound of Formula II, 200 ±10 mg emtricitabine, 28 ±1.5 mg tenofovir alafenamide hemifumarate, 360 ±15 mg Microcrystalline cellulose, 50 ± 4 mg Croscarmellose sodium, and 10.5 ± 1.5 mg magnésium stearate and wherein the total weight of the tablet is at least about 685 mg and is less than about 715 mg.

[0216] In one embodiment a tablet is provided comprising 52 ± 6 mg of the compound of Formula II, 200 ±20 mg emtricitabine, 28 ±3 mg Tenofovir alafenamide hemifumarate, 360 ±30 mg Microcrystalline cellulose, 50 ± 8 mg Croscarmellose sodium, and 8.6 ± 3 mg magnésium stearate and wherein the total weight of the tablet is at least about 685 mg.

[0217] In one embodiment a tablet is provided comprising 52 ± 6 mg of the compound of Formula Π, 200 ± 10 mg emtricitabine, 28 ±1.5 mg Tenofovir alafenamide hemifumarate, 360 ±15 mg Microcrystalline cellulose, 50 ±4 mg Croscarmellose sodium, and 8.6 ± 1.5 mg magnésium stearate and wherein the total weight of the tablet is at least about 685 mg and is less than about 715 mg.

[0218] In one embodiment, the tablet has a total weight of 700 ± 75 mg, or 700 ± 25 mg, or 700 ± 10 mg, or 700 mg. In one embodiment, the tablet is uncoated and has a total weight of about 700 ±75 mg, or about 700 ± 25 mg, or about 700 ± 10 mg, or about 700 mg. In one embodiment, the tablet has a total weight of about 720 ± 75 mg, or about 720 ± 25 mg, or about 720 ± 10 mg, or about 720 mg, or about 721 mg, or about 722 mg, or about 723 mg, or about 724 mg, or about 725 mg, or about 726 mg, or about 727 mg, or about 728 mg, or about 729 mg, or about 730 mg.

[0219] In one embodiment a tablet is provided comprising 80 ± 6 mg of the compound of Formula II, 200 ± 20 mg emtricitabine, 28 ± 3 mg Tenofovir alafenamide hemifumarate, 360 ± 30 mg Microcrystalline cellulose, 50 ± 8 mg Croscarmellose sodium, and i 1 ± 3 mg magnésium stearate and wherein the total weight ofthe tablet is at least about 715 mg.

[0220] In one embodiment a tablet is provided comprising 78 ± 2.3 mg of the compound of Formula II, 200 ± 10 mg emtricitabine, 28 ±1.5 mg Tenofovir alafenamide hemifumarate, 361 ±15 mg Microcrystalline cellulose, 51 ± 4 mg Croscarmellose sodium, and 11 ± 1.5 mg magnésium stearate and wherein the total weight of the tablet is at least about 715 mg and is less than about 745 mg.

[0221 ] In one embodiment, the tablet has a total weight of 730 ± 75 mg, or 730 ± 25 mg, or 730 ± 10 mg, or 730 mg. In one embodiment, the tablet has a total weight of about 750 ±75 mg, or about 750 ± 25 mg, or about 750 ± 10 mg, or about 750 mg, or about 751 mg, or about 752 mg, or about 753 mg, or about 754 mg, or about 755 mg.

[0222] In one embodiment, a tablet is provided comprising:

Ingrédient	Mass (mg)
The compound of Formula II	52 ±6
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	360 ±30
Croscarmellose sodium	50 ±8
Magnésium stéarate	10.5 ±3

[0223] In one embodiment, a tablet is provided comprising:

Ingrédient	Mass (mg)
The compound of Formula II	52.5 ±3
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28.1 ±1.5
Microcrystalline cellulose	360 ± 15
Croscarmellose sodium	50 ±4
Magnésium stéarate	10.5 ± 1.5

[0224] In one embodiment, a tablet is provided including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	50 ±5
Emtricitabine	200 ±20
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	25 ±3
Microcrystalline cellulose	360 ±30
Croscarmellose sodium	50 ±8
Magnésium stéarate	8.6±3

[0225] In one embodiment, a tablet is provided including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	50 ±2.5
Emtricitabine	200 ±10
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	25 ±1.5
Microcrystalline cellulose	360 ± 15
Croscarmellose sodium	50 ±4
Magnésium stéarate	8.6 ± 1.5

[0226| In one embodiment, a tablet is provided including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	52 ±6
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	360 ±30
Croscarmellose sodium	50 ±8
Magnésium stéarate	8.6 ±3

[0227] In one embodiment, a tablet is provided including:

Ingrédient	Mass (mg)
The compound of Formula II	52 ±6
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	360 ±30
Croscarmellose sodium	50 ±8
Magnésium stéarate	8.6±3

[0228] In one embodiment, a tablet is provided including:

Ingrédient	Mass (mg)
The compound of Formula Π	52.5 ±3
Emtricitabine	200 ± 10
Tenofovir alafenamide hemifumarate	28.1 ±1.5
Microcrystalline cellulose	360 ± 15
Croscarmellose sodium	50 ±4
Magnésium stéarate	8.6± 1.5

[02291 In one embodiment, a tablet is provided comprising a first layer consisting of:

Ingrédient	Mass (mg)
The compound of Formula Π	50 ± 6
Microcrystalline cellulose	250 ±20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

and a second layer consisting of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	5.6 ±1.1

[0230] In one embodiment, a tablet is provided comprising a first layer including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	50 ±6
Mîcrocrystalline cellulose	250 ± 20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

and a second layer including:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	25 ±3
Mîcrocrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	4 ±1.1

[02311 In one embodiment, a tablet is provided comprising a first layer including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	50 ±6
Microcrystalline cellulose	250 ±20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

and a second layer including:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	4 ±1.1

[0232] In one embodiment, a tablet is provided comprising a first layer including:

Ingrédient	Mass (mg)
The compound of Formula II	50 ±6
Microcrystalline cellulose	250 ± 20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

and a second layer including:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	4 ±1.1

[0233] In one embodiment, a tablet is provided comprising a first layer consisting of:

Ingrédient	Mass (mg)
The compound of Formula II	52 ±2.8
Microcrystalline cellulose	246 ±5
Croscarmellose sodium	19 ± 1.75
Magnésium stéarate	5 ±0.5

and a second layer consisting of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28 ±1.4
Microcrystalline cellulose	113 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	5.6 ±0.5

[0234| In one embodiment, a tablet is provided comprising a first layer including:

Ingrédient	Mass (mg)
The compound of Formula I, or a pharmaceutically acceptable sait therof	50 ±3
Microcrystalline cellulose	246 ±5
Croscarmellose sodium	19± 1.75
Magnésium stéarate	5 ±0.5_________

and a second layer including:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide, or a pharmacetuically acceptable sait thereof	25 ±3
Microcrystalline cellulose	H3 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	4 ±0.5

[0235] In one embodiment, a tablet is provided comprising a first layer including.

Ingrédient	Mass (mg)
The compound of Formula II	52 ±2.8
Microcrystaliine cellulose	246 ±5
Croscarmellose sodium	19 ± 1.75
Magnésium stéarate	5 ±0.5

and a second layer including:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28 ± 1.4
Microcrystaliine cellulose	113 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	4 ±0.5

[0236| In one embodiment, a tablet is provided comprising:

Ingrédient	Mass (mg)
The compound of Formula II	80 ±6
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	360 ±30
Croscarmellose sodium	50 ±8
Magnésium stéarate	ll±3

[0237] In one embodiment, a tablet is provided comprising:

Ingrédient	Mass (mg)
The compound of Formula II	78.6 ± 2.3
Emtricitabine	200 ±10
Tenofovir alafenamide hemifumarate	28.1 ±1.5
Microcrystalline cellulose	361 ±15
Croscarmellose sodium	51 ±4
Magnésium stéarate	11 ± 1.5

[0238] In one embodiment, a tablet is provided comprising a first layer consisting of:

Ingrédient	Mass (mg)
The compound of Formula Π	80 ±6
Microcrystalline cellulose	250 ±20
Croscarmellose sodium	20 ±5
Magnésium stéarate	5± 1.5

and a second layer consisting of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±20
Tenofovir alafenamide hemifumarate	28 ±3
Microcrystalline cellulose	113 ±9
Croscarmellose sodium	30 ±3
Magnésium stéarate	5.6 ±1.1

[0239| In one embodiment, a tablet is provided comprising a first layer consisting of:

Ingrédient	Mass (mg)
The compound of Formula II	78 ±2.8
Microcrystalline cellulose	247 ±5
Croscarmellose sodium	21 ± 1.75
Magnésium stéarate	5 ±0.5

and a second layer consisting of:

Ingrédient	Mass (mg)
Emtricitabine	200 ±10
Tenofovir alafenamide hemifiimarate	28 ±1.4
Microcrystalline cellulose	113 ±4
Croscarmellose sodium	30 ±1.4
Magnésium stéarate	5.6 ±0.5

[0240] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula I, or a pharmaceutically acceptable sait thereof	5-10
Microcrystalline cellulose	26-44
Croscarmellose sodium	2-4
Magnésium stéarate	0.5-0.9

and a second layer including:

	% w/w (in tablet)
Emtricitabine	21-36
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	3-5
Microcrystalline cellulose	12-20
Croscarmellose sodium	3-6
Magnésium stéarate	0.3-07

and optionaîly a film coating.

[0241] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula II	5-10
Microcrystalline cellulose	26-44
Croscarmellose sodium	2-4
Magnésium stéarate	0.5-0.9

and a second layer including:

	% w/w (in tablet)
Emtricitabine	21-36
Tenofovir alafenamide hemifumarate	3-5
Microcrystalline cellulose	12-20
Croscarmellose sodium	3-6
Magnésium stéarate	0.3-07

and optionaîly a film coating.

[0242] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula L, or a pharmaceutically acceptable sait thereof	6-9
Microcrystalline cellulose	30-40
Croscarmellose sodium	2.4-3.2
Magnésium stéarate	0,6-0.8

and a second layer including:

	% w/w (in tablet)
Emtricitabine	24-33
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	3.4-4.6
Microcrystalline cellulose	14-19
Croscarmellose sodium	3.6-4.9
Magnésium stéarate	0.6-08

and optionally a film coating.

[0243| In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula Π	6-9
Microcrystalline cellulose	30-40
Croscarmellose sodium	2.4-32
Magnésium stéarate	06-0.8

and a second layer including:

	% w/w (in tablet)
Emtricitabine	24-33
Tenofovir alafenamide hemifiimarate	3.4-4.6
Microcrystalline cellulose	14-19
Croscarmellose sodium	3.6-4.9
Magnésium stéarate	0.6-08

and optionally a film coating.

[0244] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula II	7-8
Microcrystalline cellulose	32-37
Croscarmellose sodium	2.7-29
Magnésium stéarate	0.6-08

and a second layer including:

	% w/w (in tablet)
Emtricitabine	26-30
Tenofovir alafenamide hemifumarate	3.7-4.6
Microcrystalline cellulose	15-17
Croscarmellose sodium	4.0-45
Magnésium stéarate	0.5-0.6

and optionally a film coating.

[0245] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula Π	7-8
Microcrystalline cellulose	33-36
Croscarmellose sodium	2.7-2.8
Magnésium stéarate	0.7-0.8

and a second layer including:

	% w/w (in tablet)
Emtricitabine	27-29
Tenofovir alafenamide hemifumarate	3.8-4.2
Microcrystalline cellulose	16-17
Croscarmellose sodium	4.1-4.4
Magnésium stéarate	0.5-08

and optionally a film coating.

[0246] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula Π	7.5
Microcrystalline cellulose	35.2
Croscarmellose sodium	2.8
Magnésium stearate	0.7

and a second layer including:

	% w/w (in tablet)
Emtricitabine	28.6
Tenofovir alafenamide hemifumarate	4.0
Microcrystalline cellulose	16.4
Croscarmellose sodium	4.3
Magnésium stearate	0.5

and optionally a film coating.

[02471 In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in tablet)
The compound of Formula II	7.5
Microcrystalline cellulose	35.2
Croscarmellose sodium	2.8
Magnésium stearate	0.7

and a second layer including:

	% w/w (in tablet)
Emtricitabine	28.6
Tenofovir alafenamide hemifumarate	4.0
Microcrystalline cellulose	16.2
Croscarmellose sodium	4.3
Magnésium stearate	0.8

and optionally a film coating.

[0248] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula I, or a pharmaceutically accpetable sait thereof	Il-l9
Microcrystalline cellulose	51-86
Croscarmellose sodium	4-7
Magnésium stéarate	0.5-09
Extragranular
Microcrystalline cellulose	7-12
Magnésium stéarate	0.5-0.9

and a second layer including;

	% w/w (in layer)
Intergranular
Emtricitabine	39-67
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	5-10
Microcrystalline cellulose	22-38
Croscarmellose sodium	6-10
Magnésium stéarate	0.3-0.7
Intergranular
Magnésium stéarate	0.3-07

and optionally a film coating.

[0249] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula Π	ll-I9
Microcrystalline cellulose	51-86
Croscarmellose sodium	4-7
Magnésium stéarate	0.5-0.9
Extragranular
Microcrystalline cellulose	7-12
Magnésium stéarate	05-0.9

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	39-67
Tenofovir alafenamide hemifumarate	5-10
Microcrystalline cellulose	22-38
Croscarmellose sodium	6-10
Magnésium stéarate	0.3-0.7
Intergranular
Magnésium stéarate	0.3-0.7

and optionally a film coating.

[0250] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula I, or a pharmaceutically acceptable sait thereof	13-17
Microcrystalline cellulose	58-79
Croscarmellose sodium	4.6-63
Magnésium stéarate	0.6-0.8
Extragranular
Microcrystalline cellulose	5-ll
Magnésium stéarate	0.6-0.8

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	45-61
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	6-9
Microcrystalline cellulose	25-35
Croscarmellose sodium	6.5-9.2
Magnésium stéarate	0.4-0.5
Intergranular
Magnésium stéarate	0.4-0.5

and optionally a film coating.

[0251} In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula Π	13-17
Microcrystalline cellulose	58-79
Croscarmellose sodium	4.6-6.3
Magnésium stéarate	0.6-0.8
Extragranular
Microcrystalline cellulose	5-11
Magnésium stéarate	06-0.8

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	45-61
Tenofovir alafenamide hemifumarate	6-9
Microcrystalline cellulose	25-35
Croscarmellose sodium	6.5-92
Magnésium stéarate	0.4-0.5
Intergranular
Magnésium stéarate	0.4-0.5

and optionally a film coating

[02521 In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula I, or a pharmaceutically acceptable sait thereof	14-16
Microcrystalline cellulose	66-73
Croscarmellose sodium	5.2-5.8
Magnésium stéarate	0.65-0.75
Extragranular
Microcrystalline cellulose	8-10
Magnésium stéarate	0.65-0.75

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	50-56
Tenofovir alafenamide, or a pharmaceutically acceptable sait thereof	7-8
Microcrystalline cellulose	28-32
Croscarmellose sodium	7.5-8.5
Magnésium stéarate	0.45-0.55
Intergranular
Magnésium stéarate	0.45-0.55

and optionally a film coating.

[0253] In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula Π	14-16
Microcrystalline cellulose	66-73
Croscarmellose sodium	5.2-5.8
Magnésium stéarate	0.65-0.75
Extragranular
Microcrystalline cellulose	8-10
Magnésium stéarate	0.65-0.75

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	50-56
Tenofovir alafenamide hemifumarate	7-8
Microcrystalline cellulose	28-32
Croscarmellose sodium	75-8.5
Magnésium stéarate	0.45-0.55
Intergranular
Magnésium stéarate	0,45-0,55

and optionally a film coating.

|0254J In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula II	15
Mîcrocrystalline cellulose	69
Croscarmellose sodium	5.5
Magnésium stéarate	0.7
Extragranular
Microcrystalline cellulose	9
Magnésium stéarate	0.7

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	53
Tenofovir alafenamide hemifumarate	7.5
Microcrystalline cellulose	30
Croscarmellose sodium	8
Magnésium stéarate	0.5
Intergranular
Magnésium stéarate	0.5

and optionally a film coating.

[0255| In one embodiment, a tablet is provided comprising a first layer including:

	% w/w (in layer)
Intergranular
The compound of Formula Π	15
Microcrystalline cellulose	69
Croscarmellose sodium	5.5
Magnésium stéarate	0.7
Extragranular
Microcrystalline cellulose	9
Magnésium stéarate	0.7

and a second layer including:

	% w/w (in layer)
Intergranular
Emtricitabine	53
Tenofovir alafenamide hemifumarate	7.5
Microcrystalline cellulose	30
Croscarmellose sodium	8
Magnésium stéarate	0.7
Intergranular
Magnésium stéarate	0.7

and optionally a film coating.

[0256] In one embodiment, a tablet is provided comprising a first layer including:

	Mass (mg)	% w/w (in layer)
Intragranular
The compound of Formula Π	52.5	16.24
Microcrystalline cellulose	149.9-246.3	46.41-76.26
Croscarmellose sodium	19.4	8.00
Magnésium stéarate	1.98-2.45	0.6-1.0
Extragranular
Microcrystalline cellulose	0-97.0	0-30
Magnésium stéarate	1.98-2.45	0.6-1.0

and a second layer including:

	Mass (mg)	% w/w (in layer)
Intergraniüar
Emtricitabine	200.00	53.05
Tenofovir alafenamide hemifumarate	28.1	7.45
Microcrystalline cellulose	113.2	30
Croscarmellose sodium	30.2	8.00
Magnésium stéarate	1.93-2.85	0.50-0,75
Extragranular
Magnésium stéarate	1.93-2.85	0.50-0.75

and optionally a film coating.

|0257J In one embodiment, a tablet is provided comprising a first layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
The compound of Formula Π	52.5	16.24
Microcrystalline cellulose	149.9-246.3	46,41-76.26
Croscarmellose sodium	19.4	8.00
Magnésium stéarate	l.98-2.45	0.6-1.0
Extragranular
Microcrystalline cellulose	0-97.0	0-30
Magnésium stéarate	I.98-2.45	06-1.0

and a second layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
Emtricitabine	200.00	53.05
Tenofovir alafenamide hemifumarate	28.1	7.45
Microcrystalline cellulose	113.2	30
Croscarmellose sodium	30.2	8,00
Magnésium stearaîe	1.93-2.85	0.50-0.75
Extragranular
Magnésium stéarate	1,93-2,85	0,50-0.75

and optionally a film coating.

[0258] In one embodiment, a tablet is provided comprising a first layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
The compound of Formula Π	78.7	22.3
Microcrystalline cellulose	212.5	60.2
Croscarmellose sodium	21.2	6.00
Magnésium stéarate	2.65	0.75
Extragranular
Microcrystalline cellulose	35.30	10.0
Magnésium stéarate	2.65	0.75

and a second layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
Emtricitabine	200.00	53.05
Tenofovir alafenamide hemifumarate	28.0	7.4
Microcrystalline cellulose	113.2	30
Croscarmellose sodium	30.2	8.00
Magnésium stéarate	2,83	0.75
Extragranular
Magnésium stéarate	2.83	0,75

and optionally a film coating.

[02591 In one embodiment, a tablet is provided comprising a first layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
The compound of Formula II	52.5	I6.25
Microcrystaliine cellulose	0-246.3	0-76.25
Croscarmellose sodium	19.4	8.00
Magnésium stéarate	l.98-2.45	0.6-1.0
Extragranular
Microcrystaliine cellulose	0-97.0	0-30
Magnésium stéarate	2.45	0.6-1,0

and a second layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
Emtricitabine	200,00	53.05
Tenofovir alafenamide hemifumarate	28.1	7.45
Microcrystaliine cellulose	113.2	30
Croscarmellose sodium	30.2	8.00
Magnésium stéarate	2.85	0.75
Extragranular
Magnésium stéarate	2.85	0.75

and a film coating consisting of 21 mg of Opadry II Brown 85F165072 (a combination of polyvinyl alcohol, polyethylene glycol (PEG), talc, titanium dioxide, iron oxide red, and iron oxide black).

[0260] In one embodiment, a tablet is provided comprising a first layer including:

	Mass (mg)	% w/w (in layer)
Intergranular
The compound of Formula Π	52.5	13-21
Microcrystalline cellulose	0-246	0-59
Croscarmellose sodium	19	5-25
Magnésium stéarate	2.0-2,5	0.5-3.2
Extragranular
Microcrystalline cellulose	0-97	0-30
Magnésium stéarate	2.0-2.5	0.5-3.2

and a second layer including:

	Mass (mg)	% w/w (in layer)
Intergranular
Emtricitabine	200	35
Tenofovir alafenamide hemifumarate	28	4.9
Microcrystalline cellulose	113	20
Croscarmellose sodium	30	5.2
Magnésium stéarate	2.9	0.5
Extragranular
Magnésium stéarate	2.9	0.5

and a film coating consisting of 21 mg of Opadry II Brown 85F165072 (a combination of polyvinyl alcohol, polyethylene glycol (PEG), talc, titanium dioxide, iron oxide red, and iron oxide black).

[0261J In one embodiment, a tablet is provided comprising a first layer including:

	Mass (mg)	% w/w (in tablet)
The compound of Formula Π	47-58	6.75-8.25
Microcrystalline cellulose	222-271	32-59
Croscarmellose sodium	17-21	2.5-3.1
Magnésium stéarate	4.4-5.4	06-0.8

and a second layer including:

	Mass (mg)	% w/w (in tablet)
Emtricitabine	180-220	26-31
Tenofovir alafenamide hemifumarate	25-31	3.6-4.4
Microcrystalline cellulose	104-127	15-18
Croscarmellose sodium	27-33	2.6-47
Magnésium stéarate	3.4-60	0.4-1.0

and optionaîly a film coating (e.g., Opadry II Brown 85F165072 (a combination of polyvinyl alcohol, polyethylene glycol (PEG), talc, titanium dioxide, iron oxide red, and iron oxide black).

[0262] In one embodiment, a tablet is provided comprising a first layer including:

	Mass (mg)	% w/w (in tablet)
The compound of Formula II	52.5	7.5
Microcrystalline cellulose	246	35
Croscarmellose sodium	19	2.8
Magnésium stéarate	5	0.7

and a second layer including:

	Mass (mg)	% w/w (in tablet)
Emtricitabine	200	29
Tenofovir alafenamide hemifumarate	28	4
Microcrystalline cellulose	115	16
Croscarmellose sodium	30	4.3
Magnésium stéarate	4	0.5

and optionaîly a film coating (e.g., Opadry Π Brown 85F165072 (a combination of polyvinyl alcohol, polyethylene glycol (PEG), talc, titanium dioxide, iron oxide red, and iron oxide black).

[0263] In one embodiment, a tablet is provided comprising a first layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
The compound of Formula II	78.6	22.3
Microcrystalline cellulose	212.5	60.2
Croscarmellose sodium	21.2	6.00
Magnésium stéarate	2.65	0.75
Extragranular
Microcrystaliine cellulose	35.3	10.0
Magnésium stéarate	2.65	0.75

and a second layer consisting of:

	Mass (mg)	% w/w (in layer)
Intergranular
Emtricitabine	200.00	53.05
Tenofovir alafenamide hemifumarate	28.1	7.45
Microcrystalline cellulose	113,1	30
Croscarmellose sodium	30.2	8.00
Magnésium stéarate	2.85	0.75 (0.5-0.75)
Extragranular
Magnésium stéarate	2.83	0.75 (0.5-7.5)

and a film coating consisting of 21.9 mg of Opadry II Yellow 85F92259 (a combination of polyvinyl alcohol, polyethylene glycol (PEG), talc, titanium dioxide and iron yellow).

[0264| In one embodiment, a composition is provided including about 75 mg of a compound of Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula II), about 25 mg tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and about 200 mg emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean AUCi^t of each component following a single dose administered to a human is from about 81700 h-ng/mL to about 140000 h-ng/mL of the compound of Formula I, from about 7500 h-ng/mL to about 15000 h-ng/mL of emtricitabine, and from about 165 h-ng/mL to about 400 h-ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer.

[0265] In one embodiment, a composition is provided including a compound of 50 mg Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula Π), 25 mg tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean AUC|_Mt of each component following a single dose is from about 87,000 h-ng/mL to about 131,000 h-ng/mL of the compound of Formula I, from about 8500 h-ng/mL to about 12,800 h-ng/mL of emtricitabine, and from about 186 h-ng/mL to about 227 h-ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. In certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0266] In one embodiment, a composition is provided including a compound of Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula Π), tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean AUCmf of each component following a single dose administered to a human is from about 84450 h-ng/mL to about 141000 h-ng/mL of the compound of Formula I, from about 8100 h-ng/mL to about 136000 h-ng/mL of emtricitabine, and from about 200 h-ng/mL to about 500 h-ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. In certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0267] In one embodiment, a composition is provided including a compound of Formula I (e.g., a compound of Formula II), tenofovir alafenamide, and emtricitabine, wherein the mean C_max of each component following a single dose is from about 90,000 h-ng/mL to about 135,000 h-ng/mL ofthe compound of Formula I, from about 8,700 h-ng/mL to about 13,000 h-ng/mL of emtricitabine, and from about 200 h-ng/mL to about 300 h-ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. In certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0268] In one embodiment, a composition is provided including a compound of Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula Π), tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean C_m3X of each component following a single dose administered to a human is from about 4200 ng/mL to about 8000 mg/mL of the compound of Formula L from about 1770 ng/mL to about 2800 ng/mL of emtricitabine, and from about 185 ng/mL to about 315 ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. In certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0269] In one embodiment, a composition is provided including a compound of Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula Π), tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean of each component following a single dose is from about 4700 ng/mL to about 5300 ng/mL of the compound of Formula L, from about 2000 ng/mL to about 2600 ng/mL of emtricitabine, and from about 200 ng/mL to about 300 ng/mL of tenofovir alafenamide. In certain embodiments, the composition is administered to the human in a fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. in certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0270| In one embodiment, a composition is provided including a compound of Formula I, or a pharmaceutically acceptable sait thereof (e.g., a compound of Formula II), tenofovir alafenamide, or a pharmaceutically acceptable sait thereof, and emtricitabine, or a pharmaceutically acceptable sait thereof, wherein the mean AUCiast, AUCj_nf, and/or of each component following a single dose administered to a human in the fed State is 80-125%, 80-120%, 85-115%, 90-110%, or 95-105% of the mean AUC|_ast, AUCinf, and/or Cn^x of each component following a single dose administered to a human in the fasted State. In certain embodiments, the composition is a tablet. In certain embodiments, the tablet is a bilayer tablet. In certain embodiments, the tablet is a bilayer tablet, with the Compound of Formula I in one layer and emtricitabine and tenofovir alafenamide in the other layer. In certain embodiments, the subject is fasted. In other embodiments, the subject is fed.

[0271] In certain embodiments, the pharmacokinetic profile of the compositions described herein is within acceptable ranges, regardless of whether the subject has eaten prior to taking the médication. Accordingly, in certain embodiments, the compositions described herein can be taken without regard to food intake by the subject. In certain embodiments, food intake is a low fat, moderate fat, or high fat meal.

[0272] In one embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCiast of a single dose following administration to fed patients is within about 100% to about 160% of the mean AUCiast of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCiast of a single dose following administration to fed patients is within about 100% to about 145% of the mean AUC^si of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUC|_sst of a single dose following administration to fed patients is within about 100% to about 135% of the mean AUCj_ast of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCi₃₅, of a single dose following administration to fed patients is within about 100% to about 125% of the mean AUC[_asl of a single dose following administration to fasted patients.

[0273] In certain embodiments, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUC|_3St of a single dose following administration to fed patients is within 60% of the mean AUCi_ast of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUC|_m! of a single dose following administration to fed patients is within 45% of the mean AUCiast of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCi_asl of a single dose following administration to fed patients is within 35% of the mean AUCiast of a single dose following administration to fasted patients. In another embodiments a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCi_as! of a single dose following administration to fed patients is within 25% ofthe mean AUCi^t of a single dose following administration to fasted patients.

|0274] In one embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCi_nf of a single dose following administration to fed patients is within about 100% to about 160% of the mean AUC,_nf of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCj_nf of a single dose following administration to fed patients is within about 100% to about 150% of the mean AUCj_nf of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCinf of a single dose following administration to fed patients is within about 100% to about 135% of the mean AUCinf of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCi_nf of a single dose following administration to fed patients is within about 100% to about 125% of the mean AUCinf of a single dose following administration to fasted patients.

(0275] In certain embodiments, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUC_inf of a single dose following administration to fed patients is within 60% of the mean AUCinf of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCinf of a single dose following administration to fed patients is within 50% of the mean AUCinf of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCinf of a single dose following administration to fed patients is within 35% of the mean AUCinf of a single dose following administration to fasted patients. In another embodiments a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean AUCinf of a single dose following administration to fed patients is within 25% of the mean AUCinf of a single dose following administration to fasted patients.

(0276] In one embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean C_niax of a single dose following administration to fed patients is within about 100% to about 160% of the mean C_miX of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean C^ of a single dose following administration to fed patients is within about 100% to about 130% of the mean C^ux of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean C,_m:i of a single dose following administration to fed patients is within about 100% to about 120% of the mean C_mM of a single dose following administration to fasted patients. In another embodiment, a composition 73 comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean Cmax of a single dose following administration to fed patients is within about 100% to about 115% of the mean CY of a single dose following administration to fasted patients.

[0277] In certain embodiments, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean Cmax of a single dose following administration to fed patients is within 60% of the mean Cmax of a single dose following administration to fasted patients. In another embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean Cmax of a single dose following administration to fed patients is within 30% of the mean Cmax of a single dose following administration to fasted patients. In a further embodiment, a composition comprising 50 mg Compound of Formula I, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean Cmax of a single dose following administration to fed patients is within 20% of the mean C_mJX of a single dose following administration to fasted patients. In another embodiments a composition comprising 50 mg Compound of Formula L, 25 mg tenofovir alafenamide, and 200 mg emtricitabine is provided, wherein the mean Cmax of a single dose following administration to fed patients is within 15% of the mean Cmax of a single dose following administration to fasted patients.

Manufacturing methods

[0278| Methods for producing the compositions and dosage forms (in particular tablets) disclosed herein are also provided. In some embodiments, the method comprises (a) compressing the compound of Formula I or a pharmaceutically acceptable sait thereof as a first layer, and (b) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof as a second layer. For example, in some embodiments, the method comprises (a) compressing the compound of Formula I or a pharmaceutically acceptable sait thereof as a first layer, followed by (b) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof as a second layer. In other embodiments, the method comprises (a) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof followed by (b) compressing the compound of Formula I or a pharmaceutically acceptable sait thereof as another layer. In other embodiments, the method comprises (a) compressing the compound of Formula I or a pharmaceutically acceptable sait thereof as one layer followed by (b) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof as another layer. The first layer and second layer may be compressée! separately and subsequently combined. However, more typically, a first layer is formed by compression and subsequently a second layer is compressed onto the first layer. In certain embodiments, the choice of layer order in the tableting of multilayer tablets may hâve an impact on the properties of the tablets (e.g. the adhesion of the layers within the tablet).

[0279[ In some embodiments, a tablet is provided wherein the first layer is obtainable by a method of (a) compressing the compound of Formula I or a pharmaceutically acceptable sait thereof as a first layer, and (b) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof as a second layer. In other embodiments, a tablet is provided wherein the second layer is obtainable by a method of (a) compressing the compound of Formula 1 or a pharmaceutically acceptable sait thereof as a first layer, and (b) compressing the tenofovir alafenamide or a pharmaceutically acceptable sait thereof and emtricitabine or a pharmaceutically acceptable sait thereof as a first layer.

|0280] In certain embodiments, the methods will include a step of coating the tablet cores after compression, e.g. with a film coating as described above.

[02811 In general, tableting methods are well known in the art of pharmacy. Techniques and formulations generally are found in Remington's Pharmaceutical Sciences (Mack Publishing Co., Easton, PA), which is hereby incorporated by reference herein in its entirety.

[0282J A tablet can be made by compression or molding, optionally with one or more excipients. Compressed tablets may be prepared by compressing in a suitable machine the active ingrédient in a free-flowing form such as a powder or granules, optionally mixed with excipients.

Therapeutic methods

[02831 The solid oral dosage forms (in particular tablets) disclosed herein are used for treatment of HIV infection (e.g. HTV-1 infection). In certain embodiments, the solid oral dosage forms (in particular tablets) disclosed herein are used for pre-exposure prophylaxis (PrEP) to reduce the risk of sexually acquired HIV-1.

[0284) Accordingly, methods for treating a subject infected with HIV are provided, comprising administering a sohd oral dosage form disclosed herein (in particular a tablet) to the subject. Similarly, a solid oral dosage form (in particular a tablet) is provided for use in such treatment methods. Also provided is the use of the compound of Formula I or a pharmaceutically acceptable sait thereof, tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof, in the manufacture of an oral dosage form disclosed herein (in particular a tablet) for treatment of HIV infection. In some embodiments, the invention provides the use of tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof, in the manufacture of an oral dosage form disclosed herein (in particular a tablet) for treatment of HTV infection.

[0285| In certain embodiments, the solid oral dosage forms (in particular tablets) disclosed herein are used for pre-exposure prophylaxis (PrEP) to reduce the risk of sexually acquired HIV-l. Accordingly, methods for preventing infection in a subject at risk of infection with HIV are provided, comprising administering a solid oral dosage form disclosed herein (in particular a tablet) to the subject. Similarly, a solid oral dosage form disclosed herein (in particular a tablet) is provided for use in such treatment methods. The invention also provides the use of the compound of Formula I or a pharmaceutically acceptable sait thereof, tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof, in the manufacture of an oral dosage form disclosed herein (in particular a tablet) for prévention of HIV infection in a subject at risk for infection. In some embodiments, the invention provides the use of tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and emtricitabine or a pharmaceutically acceptable sait thereof, in the manufacture of an oral dosage form disclosed herein (in particular a tablet) for prévention of HIV infection.

[0286] In certain embodiments, a method of treating a subject with HTV with the compositions disclosed herein is provided, wherein the method does not increase the risk of a drug-drug interaction in the patient. In certain embodiments, a method of treating a subject with HIV with the compositions disclosed herein is provided, wherein the method decreases the risk of a drug-drug interaction in the patient. Exemplary drug-drug interactions include interactions between the compound of Formula I, tenofovir alafenamide, or emtricitabine with rifampin, metfonmin, one or more hormonal contraceptives or an HCV antiviral agent (e.g., one or more of sofosbuvir, ledipasvir, velpatasvir, voxilaprevir, grazoprevir, boceprevir, elbasvir, dasabuvir, ombitasvir, paritaprevir, ABT-530, and ABT-493). In certain embodiments, a method of treating a subject with HIV is provided, wherein the subject is administered one or the compositions disclosed herein, wherein the subject is also being treated with rifampin, metformin, or an HCV antiviral agent (e.g., one or more of sofosbuvir, ledipasvir, velpatasvir, voxilaprevir, grazoprevir, boceprevir, elbasvir, dasabuvir, ombitasvir, paritaprevir, ABT-530, and ABT-493).

[0287j The methods involve administering an oral dosage form disclosed herein (in particular a tablet) to the subject, typically a human, and will generally involve repeated administrations, typically once daily. The treatment may be prophylactic or therapeutîc treatment.

General |0288] The term “fed” in relation to administration of a solid oral dosage form to a human subject means administration of the dosage form orally under fed conditions (moderate fat meal) e.g. administration within about 30 minutes of the human consuming a standardized meal of about 300 to 76

600 calories and about 10 to about 15 grains of fat. In some embodiments, “fed” refers to administration within about 30 minutes of the human consuming a high fat meal.

[0289] The term “substantially free” in relation to the presence of a given component within e.g. a composition means that less than 5% by weight ofthe composition (e.g. less than 1% by weight of the composition) is that given component. The word “substantially” does not exclude “completely” e.g. a composition which is “substantially free” from Y may be completely free from Y. Where necessary, the word “substantially” may be omitted from the définition of the invention.

[0290] The term “segregated” as used in relation to certain components (e.g. A and B) within a tablet means that those components are physically discrète such that the presence of one component (e.g. A) does not substantially affect the stability in storage of the other component(s) (e.g. B) from which it is segregated. Typically, when components are segregated in a tablet then they will be présent in separate layers in a multilayer tablet. By way of example, components A and B may be présent in separate layers in a multilayer tablet, wherein (a) the layer containing component A is substantially free of component B and (b) the layer containing component B is substantially free of component A. The separate layers may be in contact with each other or may be separated e.g. by one or more additional layers.

[02911 The term “comprise” and variations thereof, such as “comprises” and “comprising”, are to be construed in an open, inclusive sense, that is as “including, but not limited to”.

[0292j The term “between” with reference to two values includes those two values e.g. the range “between” 10 mg and 20 mg encompasses e.g. 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20 mg.

[0293[ The term “about” used in connection with a quantity is inclusive of the stated value and has the meaning dictated by the context (e.g., includes the degree of error associated with measurement of the particular quantity). For example, in certain nonlimiting example the term “about” in relation to a numerical value x refers to x+10%, x+5%, or x+1 %.

[0294| “% w/w” means the weight of a component as a percentage of the total weight of e.g. a layer or dosage form in which the component is présent. For example, a composition comprising “5% w/w X” refers to a composition in which the weight of component X is 5% of the total weight of the composition.

[0295] Reference throughout this spécification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment provided herein. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this spécification are not necessarily ail referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0296] The term pharmaceutically acceptable with respect to a substance refers to that substance which is generally regarded as safe and suitable for use without undue toxicity, irritation, allergie response, and the like, commensurate with a reasonable benefit/risk ratio. “Pharmaceutically acceptable” with regard to excipients includes without limitation any adjuvant, carrier, excipient, ghdant, sweetening agent, diluent, preservative, dye/colorant, flavor enhancer, surfactant, wetting agent, dispersing agent, suspending agent, stabilizer, isotonie agent, solvent, or emulsifier which has been approved by the United States Food and Drug Administration as being acceptable for use in hum ans or domestic animais.

[0297] “Pharmaceutically acceptable sait” refers to a sait of a compound that is pharmaceutically acceptable and that possesses (or can be converted to a form that possesses) the desired pharmacological activity of the parent compound. Such salts include acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethanesulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, lactic acid, maleic acid, malonic acid, mandelic acid, methanesulfonic acid, 2-napththalenesulfonic acid, oleic acid, palmitic acid, propionic acid, stearic acid, succinic acid, tartane acid, p-toluenesulfonic acid, trimethylacetic acid, and the like, and salts formed when an acidic proton present in the parent compound is replaced by either a métal ion, e.g., an alkali métal ion, an alkaline earth ion, or an aluminum ion, or coordinates with an organic base such as diethanolamine, triethanolamine, Nmethylglucamine and the like. Also included in this définition are ammonium and substituted or quatemized ammonium salts. Représentative non-limiting lists of pharmaceutically acceptable salts can be found m S.M. Berge et al., J. Pharma Sci., 66(1), 1-19 (1977), and Remington: The Science and Practice ofPharmacy, R. Hendrickson, ed., 21 st édition, Lippincott, Williams & Wilkins, Philadelphia, PA, (2005), at p. 732, Table 38-5, both of which are hereby incorporated by reference herein.

[0298| As used herein, the term “salts” includes co-crystals, The term ’'co-crystal refers to a crystalline compound comprising two or more molecular components, e.g. wherein proton transfer between the molecular components is partial or incomplète.

|0299J The term “solvaté” means a molecular complex comprising a compound and one or more pharmaceutically acceptable solvent molécules. Examples of solvent molécules include water and C].

alcohols, e.g. éthanol. When the solvaté is water, the term “hydrate” may be used.

[0300] “Treating” and “treatment” of a disease include the following:

(1) preventing or reducing the risk of developing the disease, i.e. causing the clinical symptoms ofthe disease not to develop m a subject that may be exposed to or predisposed to the disease but does not yet expérience or display symptoms of the disease, (2) inhibiting the disease, i.e. arresting or reducing the development of the disease or its clinical symptoms, and (3) relieving the disease, i.e. causing régression of the disease or its clinical symptoms.

The term “effective amount” refers to an amount that may be effective to elicit the desired biological or medical response, including the amount of a compound that, when administered to a subject for treating a disease, is sufficient to effect such treatment for the disease. The effective amount will vary depending on the compound, the disease and its severity and the âge, weight, etc. of the subject to be treated. The effective amount can include a range of amounts.

EXAMPLES

[0301] The following examples are provided for purposes of illustration, not limitation.

Example 1 - Compound of Formula II single agent tablets

[0302| A formulation (tablet Fl) of the compound of Formula II was prepared by dry granulation. Figure 5 is a flow diagram illustrating the préparation of this formulation. The composition of the single agent formulation is shown in the table below:

	Tablet Formulation Fl
Component	Mass (mg/tablet)	% w/w/ tablet
Compound of Formula II	78.7*	26.2
Microcrystalline cellulose	120	40
Lactose monohydrate	75.8	25.3
Crospovidone	21	7
Sodium Stearyl Fumarate**	4.5	1.5
Total Weight	300

•Equivalent to 75 mg ofthe Compound of Formula I ** Intergranular2.25 mg (0.75%; Extragranular: 2.25 mg (0.75%) |0303] In the pharmacokinetic studies of Example 3, the tablets of Formulation Fl were film coated with 12 mg Opadry II Yellow 85F92259. The total weight of the film coated tablets was 312 mg.

[0304] The Compound of Formula II was blended with intergranular excipients (lactose, microcrystalline cellulose, and crospovidone), further blended with the intergranular portion of the sodium stearyl fumarate), roller compacted, milled, and final blended with the sodium stearyl fumarate to yield a final powder blend for compression. The final powder blend had a mean particle size of 347 pm with acompressibility index of 21%. The final powder blend was compressed into tablet cores, which were film-coated as described above to a target weight gain of 4%.

Example 2 - Compound of Formula II / emtricitabine / tenofovir alafenamide bilayer tablets [0305] A bilayer formulation (tablet F2) of the compound of Formula Π, emtricitabine, and tenofovir alafenamide hemifumarate was prepared using the method described in Example 8. Figure 6 is a flow diagram illustrating the préparation of bilayer tablets. The composition of the formulation is summarized in the table below:

Ingrédient	Bilayer tablet F2 (mg/tablet)
Compound of Formula II Layer	Emtricitabine/ tenofovir alafenamide hemifumarate Layer
Compound of Formula Π	78.7’
Emtricitabine		200
Tenofovir alafenamide hemifumarate		28.1”
Microcrystalline cellulose	247.8*”	113.1
Croscarmellose sodium	21.2	30.2
Magnésium Stéarate	5.31	5.7n
Layer Weight	353	377
Tablet Core Weight	730

'Equivalent to 75 mg of tlie Compound of Formula I

Equivalent to 25 mg tenofovir alafenamide 'Intergranular: 212.5 mg (29. l%); Extragranular: 2.65 mg (0.4%) ⁺Intergranular: 2.65 mg (0.4%); Extragranular 2.65 mg (0.4%) ^Intergranular: 2.83 mg (0.4%); Extragranular 2.83 mg (0.4%) |0306] In the pharmacokinetic studies of Example 3, the tablets of Formulation F2 were film coated with 21.9 mg Opadry II Yellow 85F92259 (represented 3% weight gain). The total weight of the film coated tablets was 752 mg.

|0307] The tablets were manufactured using a dry granulation by roller compaction process. An emtricitabine / tenofovir alafenamide final powder blend was manufactured by dry granulation of emtricitabine and tenofovir alafenamide with excipients (microcrystalline cellulose, croscarmellose sodium, intergranular magnésium stéarate), followed by blending with extragranular lubricant (extragranular magnésium stéarate). The final powder blend of the Compound of Formula I was manufactured by dry granulation of the active component with excipients (microcrystaliine cellulose, croscarmellose sodium, intergranular magnésium stéarate) followed by blending with the extragranular 81 filler and lubricant (microcrystalline cellulose and extragranular magnésium stéarate). In this study, the emtricitabine/tenofovir alafenamide final powder blend was compressed as layer l and the final powder blend of the Compound of Formula I was compressed as layer 2 to give a final bilayer tablet cote. The core was film coated as described above.

Example 2A Emtricitabine / tenofovir alafenamide tablets |0308| The composition of the emtricitabine / tenofovir alafenamide (F/TAF) fîxed dose combination tablets used in subséquent studies is shown in the following table:

Component	Tablet Formulation F3 Emtricitabine/tenofovir alafenamide 200/25 mg (mg/tablet)
Intergranular
Emtricitabine	200
Tenofovir Alafenamide Hemifumarate	28.0
Microcrystalline Cellulose	88.7
Croscarmellose Sodium	28.0
Magnésium Stéarate	2.6
Extragranular
Magnésium Stéarate	2.6
Total Tablet Core Weight	350
Film-Coatîng
OpadrylIBlue 85F105057	10.5

[0309] Emtricitabine and tenofovir alafenamide hemifumarate were co-blended with microcrystalline cellulose and croscarmellose sodium, followed by lubrication with magnésium stéarate. The roller compaction pre-blend was then roller compacted and milled using an oscillating mill. The résultant granules were lubricated with magnésium stéarate and compressed into 350 mg tablet cores which that were subsequently film coated.

Example 3 — Pharmacokinetic Studies

[0310] Studies were carried out to assess the pharmacokinetic profiles of the formulations Fl, F2, and F3 of Examples i, 2, and 2A. A randomized, open-label, multiple-period, fixed-sequence, crossover study was performed to evaluate the relative bioavailability of bilayer tablet formulation F2 compared with that of single agent tablet formulation Fl co-administered simultaneously with a fixed-dose combination tablet containing emtricitabine and tenofovir alafenamide (tablet formulation F3). The bioavailability was evaluated in healthy subjects.

Study Design and Duration of Treatment |0311] Three single doses of the following tablet formulations were administered orally during up to 21 days total study duration:

(a) a fixed dosed combination tablet containing emtricitabine and tenofovir alafenamide (200/25 mg - Tablet F3) and a single agent tablet containing the compound of Formula I (75 mg - Tablet Fl) administered simultaneously, under fasted conditions (dosed on day 1 ; days 28 washout);

(b) a fixed dose combination tablet containing the compound of Formula I, emtricitabine, and tenofovir alafenamide (75/200/25 mg - Tablet F2) administered under fasted conditions (dosed on day 9; days 10-16 washout); and (c) a fixed dose combination tablet containing the compound of Formula I, emtricitabine, and tenofovir alafenamide (75/200/25 mg - Tablet F2) administered under fed conditions with a high-fat meal (dosed on day 17; day 21 discharge).

Criteria for Evaluation

[0312] The following plasma pharmacokinetic parameters were calculated: Cnu_X, AUCt_ast, and AUCinf.

Statistical Methods |0313| Pharmacokinetics: Plasma concentrations and PK parameters were listed and summarized by analyte and treatment group using descriptive statistics. In addition, a parametric analysis of variance using a mixed-effects model appropriate for a crossover design was fitted to the natural logarithmic transformation of the PK parameters (AUCinf, AUCiast, and Cnux). Two-sided 90% confidence intervals (CIs) were constructed for the ratio of géométrie least-squares means (GLSMs) of each PK parameter for the compound of Formula I, emtricitabine, and tenofovir alafenamide.

Results

[0314] Subject Disposition and Demographics

[0315] A total of 28 subjects were randomized and received at least 1 dose of study drug.

[0316] The results for the first cohort ofthe clinical study are summarized in the table below. This table présents the mean (%CV) values. Géométrie Least Squares Mean (GLSM) ratios, and 90% CI values of the plasma PK parameters AUC|_Mt, AUCinf, and Cmax for each active ingrédient, following administration of bilayer tablet formulation F2 or the single agent tablet formulation Fl, coadministered with a fixed dose combination of emtricitabine and tenofovir alafenamide under fasted conditions.

PK Parameter	Mean (%CV)	% GLSM Ratio (90% CI)
Compound of Formula I /F/TAF (75/200/25 mg) (Test) (N = 28)	Compound of Formula I (75 mg) + F/TAF (200/25 mg) (Reference) (N = 28)
Compound of Formula E
AUCha (h’ng/mL)	151,844.0 (26.9)	119,619.4 (26.6)	126.76(117.82, 136.37)
AUCmf (h*ng/mL)	156,637.5(27.5)	123,174.0 (26.6)	126.82 (117.87, 136.45)
Cmax (ng/mL)	7123.9 (21.6)	5593.9(31.0)	130.72(119.95, 142.45)
FTC
AUCtaa fh’iig/mL)	11,412.3 (13.5)	11,199.3(13.7)	101.89 (99,50, 104.33)
AUCmf (h’ng/mL)	11,642.8(13.2)	11,436.4(13.2)	101.78(99.45, 104.16)
Cmax (ng/mL)	2264.3 (22.7)	2153.6(21.5)	104.86 (97.73, 112.50)
TAF
AUCimi (li*ng/mL)	205.5 (45.5)	223.6 (45.2)	91.62(82.13, 102.21)
AUCmf (h«ng/mL)	206.8 (45,2)	225.1 (45.0)	91.56 (82.27, 101.91)
Cmax (ng/mL)	253.3 (44.2)	276.7(51.7)	95.47 (79.88. 114.10)

[0317] The total exposure of the compound of Formula I, under fasted conditions, was approximately 30% higher when dosed in the bilayer tablet formulation F2, containing two additional therapeutic agents (TAF and FTC), compared to single agent tablet formulation Fl, co-dosed with a fixed dose combination tablet containing TAF and FTC (F3). Under fasted conditions, the AUCmf and C^ ofthe compound of Formula I were 27% and 31% higher, respectively, following administration of bilayer tablet formulation F2 than following administration of single-agent tablet formulation Fl with the F/TAF fixed dose combination (F3, 200/25 mg). Emtricitabine and tenofovir alafenamide exposure was similar following administration of bilayer tablet formulation F2 or single-agent tablet formulation Fl with the F/TAF FDC (F3, 200/25 mg).

[0318J Based on the data from this study, a new fixed dose formulation of the compound of Formula I, emtricitabine, and tenofovir alafenamide was developed.

[0319] The effect of food on the pharmacokinetics of the compound of Formula I was also examined in a non-randomized, open label, crossover, 2 period, fixed sequence, and single dose cohort consisting 84 of 8 unique subjects. In this study, a single dose of the compound of Formula I was administered in two periods with fixed sequence under fasted and fed conditions respectively. A washout of at least five half-lives was required between fasted and fed sequences.

[0320] The compound of Formula I was prepared according to percent weight of the tablet formulation of Example 1, a dose équivalent to 100 mg of the compound of Formula I and excipient amounts adjusted accordingiy to arrive at the designated weight percentages (100 mg/tablet). A single tablet containing the compound of Formula I (100 mg/tablet) was administered with approximately 240 mL water after an ovemight fast (no food or drink, except water, for at least 10 hours) on Day 1. Subjects continued to fast until 4 hours post dose or until collection of the 4-hour postdose blood sample. On Day 9, A single tablet containing the compound of Formula I (100 mg/tablet) was administered with approximately 240 mL water after an ovemight fast (no food or drink, except water, for at least 10 hours) and within 5 minutes of the subjects finîshing a standard high-fat breakfast (approximately 800 calories with 50% of calories from fat).

[0321] The évaluation of the effect of food on compound of Formula I was obtained by comparing AUCjnf, AUC₍,-last, and Cmax on Days 1 and 9 under fed and fasted conditions. The natural logtransformed PK parameters (i.e., AUCinf, AUCo-b*, and C^x) were evaluated using a mixed-effects model with food as a fixed effect and subject as a random effect. The 90% CI for the géométrie least square mean (GLSM) ratios for fast versus fed was constructed. The statistical comparison of the pharmacokinetic parameters of the compound of Formula I following single dose administration of this tablet formulation in fed and fasted States is presented in the table below.

Compound of Formula I PK Parameter	Mean (%CV)	% GLSM Ratio (90% CI)
Test Compound of Formula I 100 mg Fed (n=8)	Reference Compound of Formula I 100 mg Fasted (n=8)
AUCmf (hr*ng/mL)	214,146.3(15.9)	117,777.1 (23.3)	183.97(152,05, 222.59)
AUCiasi (hr*ng/mL)	209.259.9(15.1)	115.681.7 (24.0)	183.58 (151.91, 221.86)
Cma.\ (ng/mL)	11.268.8(15.1)	5885.0 (34.9)	200.69(165.93,242.74)

[0322] The Table above présents the GLSM ratios and associated 90% confidence intervals (CIs) for the test (fed) versus reference (fasted) treatments for the primary plasma PK parameters of the compound of Formula I. Administration of a single dose of the compound of Formula I 100 mg with food (high-calorie/high-fat breakfast) increased the GLSM values of C™* and AUC_inr 101% (90% CI of GLSM ratio 165.93% to 242.74%) and 84% (90% CI of GLSM ratio 152.05% to 222.59%), respectively. There were no apparent changes in clearance and t_I/2 following administration with food, 85 indicating that food enhanced the bioavailability of the compound of Formula I by improving its solubility and/or absorption.

[0323] The effect of food on the PK of the compound of formula I, emtricitabine, and tenofovir alafenamide when administered as the tablet formulation F2 tablet was evaluated in the clinical study described above (The randomized, open-iabel, multiple-period, fixed-sequence, crossover study invoiving 28 subjects comparing bilayer tablet formulation F2 with that of single agent tablet formulation Fl co-administered simultaneously with a fixed-dose combination tablet containing emtricitabine and tenofovir alafenamide (tablet formulation F3).

[0324] The mean (%CV) values, GLSM ratios, and 90% CI values of the plasma for PK parameters AUC_last, AUCinf, and C_milx for the compound of Formula I, emtricitabine, and tenofovir alafenamide following administration of tablet formulation Fl under fasted conditions or with a high-fat meal are presented in the following table.

PK Parameter	Mean (%CV)	% GLSM Ratio (90% CI)
Compound of Formula I /F/TAF (75/200/25 mg) (High-Fat Meal; Test) (N = 28)	Compound of Formula I /F/TAF (75/200/25 mg) (Fasted; Reference) (N = 28)
Compound of Formula 1
AUCta (h*ng/inL)	216,733.1 (23.4)	151,844.0 (26.9)	144.45(134.26, 155.40)
AUCmf (h’ng/mL)	226,142.1 (24.9)	156,637.5 (27.5)	145.88 (135.58, 156.95)
Cmax {ng/lïlL)	8941.1 (16.9)	7123.9(21.6)	126.74(116.30, 138.12)
FTC
AUCiot (h-ng/mL)	11,483.0(15.7)	11,412.3 (13.5)	100.34 (97.99, 102.75)
AUCmi (h-ng/mL)	11,706.4(15.6)	11,641.6(13.2)	100.25 (97.96, 102.59)
C„ (ng/mL)	1872.5 (20.1)	2264.3 (22.7)	83.18(77.53,89.25)
TAF
AUCim (h-ng/mL)	315.3 (44.0)	205.5 (45.5)	156.81 (140.57, 174.94)
AUCmf (h-ng/mL)	319.7(43.1)	206.8 (45.2)	158.20(142.14, 176.08)
Cmax (Hg/ïïlL)	212.2(49.4)	253.3 (44.2)	83.22 (69.63. 99.46)

[0325] Compared with administration under fasted conditions, administration of the tablet formulation F2 with a high-fat meal resulted in a 46% higher AL’C,_nf and a 27% higher C_max for the compound of Formula I. The impact of food on emtricitabine and tenofovir alafenamide exposure was similar to that previously observed.

[0326] The data presented in the two tables above also demonstrate that the effect of food on the total exposure of the compound of Formula I is reduced in bilayer tablet formulation F2, relative to the single agent tablet formulation Fl. The géométrie mean ratio of AUC_inf for the compound of Formula I in single agent tablet formulation Fl was 1.84, while the géométrie mean ratio of AUCinf for the compound of Formula I in bilayer tablet formulation F2 was 1.46. In comparison the géométrie mean ratio of AUCinf for emtricitabine in to 0,91 and 1.00 for emtricitabine and 1.75 and 1.58 for tenofovir alafenamide in each formulation respectively (comparing the emtricitabine and tenofovir alafenamide exposures of the tablet formulation F2 with that of tablet formulation Fl codosed with the tablet formulation F3). The géométrie mean ratio of Cmax for single agent tablet formulation Fl was 2.01, while the géométrie mean ratio of C_max for bilayer tablet formulation F2 was 1.27. In comparison, the géométrie mean ratios of Cm^ for emtricitabine and tenofovir alafenamide in tablet formulations Fl and F2 were 0.85 and 0.83, respectively. These data indicate that a fixed dosed combination tablet formulation of the compound of Formula I, emtricitabine, and tenofovir alafenamide may be taken without regard to food.

Example 4 - Dissolution Studies

[0327| Studies were carried out to assess the dissolution profiles of tablets Fl, F2, and F3-A. Dissolution of the compound of Formula II was measured using USP Apparatus II, in 250 mL of pH

6.5 fasted State simulated intestinal fluid (FaSSEF), at 37 °C andpaddle speed of 100 rpm. FaSSIF was prepared by adding simulated intestinal fluid (SIF) powder (Biorelevant.com) to pH 6.5 phosphate buffer at approximately 4.48 g/L. Once the powder was dissolved, the volume of the resulting solution was doubled. In the case of the single agent tablet formulation Fl, dissolution of the compound of Formula Π was measured in the presence of the fixed dose combination tablet formulation F3 of Example 2A containing emtricitabine and tenofovir alafenamide hemifumarate. The results are shown in Figure 1. These data show that the bilayer formulation (tablet F2) exhibited enhanced dissolution of the compound of Formula IL compared to the single agent formulation (tablet Fl codosed with F3). Tablet formulation F3-A was prepared as a single agent formulation according to the process described in Example 1 according to the table below.

Ingrédient	Tablet F3-A (mg/tablet)
Compound of Formula II Layer
Compound of Formula Π	78.7*
Emtricitabine
Tenofovir alafenamide hemifumarate
Microcrystalline cellulose	247.8”·
Croscarmellose sodium	21.2
Magnésium Stearate	5.3f
Tablet Core Weight	353

Equivalent to 75 mg of the Compound of Formula I Intergranular: 212.5 mg (29. l%); Extragranular: 2.65 mg (0.4%) ^Intergranular 2.65 mg (0.4%); Extragranular: 2.65 mg (0.4%)

[0328] As shown above, Tablet F3-A used the composition (excipient sélection and quantifies) of the Compound of Formula II Layer of Example 2 (F2). Figure l further demonstrates that Tablet formulation F2 exhibited enhanced dissolution of the compound of Formula II compared to Tablet formulations Fl and F3-A. The total percentage of the compound of Formula II dissolved from formulation Fl and F3-A was comparable at approximately 60%. The total amount of the compound of Formula Π dissolved from formulation F2 was approximately 10% higher.

[0329| In a second study, dissolution of the compound of Formula Π was measured using fed State simulated intestinal fluid (FeSSIF) as dissolution medium. FeSSIF was prepared by adding simulated intestinal fluid (SIF) powder (Biorelevant.com) to pH 5.0 phosphate buffer at approximately 22.405 g/L. Once the power was dissolved, the volume of the resulting solution was doubled. In this study, dissolution of the compound of Formula Π was measured using USP Apparatus Π, in 250 mL of pH 5.5 fed simulated intestinal fluid, at 37 °C and paddle speed of 100 rpm. As in the previous study using fasted simulated intestinal fluid, the single agent tablet formulation Fl was tested in the presence of a second tablet formulation containing emtricitabine and tenofovir alafenamide hemifumarate (tablet F3). The results are shown in Figure 2. These data also show that the bilayer formulation (tablet F2) exhibited enhanced dissolution of the compound of Formula II, compared to the single agent formulation (tablet Fl).

Exaniple 5 — Excipient Studies

[0330J To further assess the effect of spécifie excipients on dissolution of the compound of Formula I, three additional bilayer tablet formulations were prepared, F4, F5, and F6. Tablet formulation F4 is similar to tablet formulation F2, with croscarmellose sodium being replaced with crospovidone. Likewise, tablet formulation F5 is similar to tablet formulation F2, with magnésium stéarate being replaced with sodium stearyl fumarate. Finally, tablet formulation F6 is similar to tablet formulation F2, with both croscarmellose sodium and magnésium stéarate being replaced with crospovidone and sodium stearyl fumarate, respectively. Tablets F4, F5, and F6 were prepared using the method described in Example 8.

Ingrédient		Bilayer tablet ' F4	Bilayer tablet F5	Bilayer tablet F6
Emtricitabine/ tenofovir alafenamide hemifiimarate Layer (mg/tablet)	Compound of Formula I Layer (mg/tablet)
Emtricitabine	200
Tenofovir alafenamide hemi fumarate	28.1
Compound of Formula II		78.7	78.7	78.7
Microcrystalline cellulose	113	244	248	244
Croscarmellose sodium	30.2		21.2
Crospovidone		24.7		24.7
Magnésium Stéarate	5.7	5.3
Sodium Stearyl Fumarate			5.3	5.3
Layer Weight	377	353	353	353
Tablet Core Weight		730	730	730

[03311 Figure 3 shows the results of dissolution studies performed on tablet formulations F4, F5, and F6, as well as tablet formulation Fl and F2. These data demonstrate that the substitution of each excipient enhances the dissolution ofthe compound of Formula I.

Example 6 - Compound of Formula I / emtricitabine / tenofovir alafenamide bilayer tablets — 50 mg dose

[0332] As a resuit of the higher in vivo exposure of the compound of Formula I that was observed in the pharmacokinetic studies, a bilayer formulation (tablet F7) of the compound of Formula II, emtricitabine, and tenofovir alafenamide hemifumarate was prepared incorporating a lower dose of the compound of Formula I. Tablet formulation F7 was prepared as described previously, i.e., using the method described in Example 8. The composition of the formulation is summarized in the table below:

Ingrédient	Bilayer tablet F7 (mg/tablet)
Compound of Formula Π Layer	Emtricitabine/ tenofovir alafenamide hemifumarate Layer
Compound of Formula II	52,5*
Emtricitabine		200
Tenofovir alafenamide hemifumarate		28
Microcrystalline cellulose	246.3	113.2
Croscarmellose sodium	19.4	30.2
Magnésium Stéarate	4.9	5.7
Layer Weight	323	377
Tablet Core Weight	700
Opadry II Brown 85F165072	21
Film Coated Tablet	721

Equivalent to 50 mg of the Compound of Formula I 'Equivalent to 25 mg tenofovir alafenamide

Example 7 — Compound of Formula I single agent tablets (50 mg)

[0333] Dissolution studies were performed comparing the 50 mg dose bilayer tablet F7 with a 50 mg single agent tablet.

[0334] A 50 mg formulation (tablet F8) of the compound of Formula Π was prepared in a similar manner as tablet formulation Fl of Example l. The composition of the single agent formulation is shown in the table below:

	Tablet Formulation F8
Component	Mass (mg/tablet)	% w/w/ tablet
Compound of Formula Π	52.5	26.2
Microcrystalline cellulose	80	40
Lactose	50.5	25.3
Crospovidone	14	7
Sodium Stearyl Fumarate	3	1.5
Total Weight	200

[0335] Figure 4 shows the dissolution of 50 mg bilayer tablet formulation (fixed dose combination) F7 and 50 mg single agent tablet formulation F8. Dissolution of the compound of Formula Π was measured using USP Apparatus II, in 333 mL of pH 6.5 FaSSIF, at 37 °C and paddle speed of 100 rpm. Two tablets of each tablet formulation, F7 and F8 were tested. As in Example 3, in the case of the single agent tablet formulation F8, dissolution ofthe compound of Formula II was measured in the presence of the fixed dose combination tablet formulation of Example 2A containing emtricitabine and tenofovir alafenamide hemifumarate. These data show that the 50 mg bilayer formulation (tablet F7) exhibited enhanced dissolution of the compound of Formula II, compared to the single agent formulation (tablet F8).

Example 8 — manufacturing process

[0336] The manufacturing/packaging procedure for the compound of Formula II/emtricitabine/tenofovir alafenamide hemifumarate tablets is divided into five unit processes:

l. mixing of the compound of Formula II drug substance with intergranular excipients, roller compaction or slugging, milling, and blending with extragranular excipients to yield the final powder blend for the compound of Formula Π;

2. mixing of emtricitabine and tenofovir alafenamide hemifumarate drug substances with intergranular excipients, dry granulation, milling, and blending with extragranular excipients to yield emtricitabine/tenofovir alafenamide hemifumarate final powder blend;

3. tablet compression to yield bilayer tablet cores;

4. tablet fdm-coating to yield film-coated tablets; and

5. packaging.

[0337J The manufacturing process steps to produce the final drug product are detailed below.

Final Powder Blend for the Compound of Formulait (Dispensing, Blending, Dry Granulation, Milling, Final Blending)

1. Weigh the compound of Formula Π and the excipients (microcrystalline cellulose and croscarmellose sodium). Correct the weight of the compound of Formula II based on the drug content factor (DCF), with a concomitant réduction in the weight of microcrystalline cellulose.

2. Blend in intergranular portion of magnésium stéarate to the tumble blender and blend.

Dry granulate the resulting blend using a roller compacter or slug the resulting blend and mill.

4. Add extragranular microcrystalline cellulose and magnésium stéarate and blend.

Emtricitabine/tenofovir alafenamide hemifumarate Final Powder Blend (Dispensing, Blending. Drv Granulation, Milling, Final Blending)

5. Weigh emtricitabine and tenofovir alafenamide hemifumarate drug substances and excipients (microcrystalline cellulose and croscarmellose sodium). Adjust the weight of emtricitabine and tenofovir alafenamide hemifumarate drug substances based on their corresponding DCF, with a concomitant adjustment to the weight of microcrystalline cellulose.

6. Blend in emtricitabine and tenofovir alafenamide hemifumarate drug substance, microcrystalline cellulose, and croscarmellose sodium to a tumble blender and blend.

7. Blend in intergranular portion of magnésium stéarate to the tumble blender and blend.

8. Dry granulate the resulting blend using a roller compactor or slug the resulting blend and mill.

9. Blend in the extragranular portion of magnésium stéarate.

Tableting

10. Compress the final powder blend of the compound of Formula II as the first layer and the emtricitabine/tenofovir alafenamide hemifumarate final powder blend as the second layer, with an appropriate main compression force to achieve a target hardness of 17 kP (range: 14 to 20 kP). For tablet formulations containing 50 mg of the compound of Formula Π, the final powder blend is compressed to a target layer weight of 323 mg using a target total tablet weight of 700 mg. For tablet formulations containing 75 mg of the compound of Formula Π, the final powder blend is compressed to a target layer weight of 353 mg using a target total tablet weight of 730 mg.

Film-coating

H. Préparé a suspension of Opadry® Π Brown 85F165072 (for tablets containing 50 mg compound of Formula I) or Opadry® Π Yellow 85F92259 (for tablets containing 75 mg compound of Formula I). Film-coat the tablet cores to achieve the target tablet weight gain of 3% (range 2-4%). Dry film-coated tablets prior to cooling and discharge.

Example 9 - Pharmacokinetic Studies — Cohort 2

[0338] The pharmacokinetic study of Example 3 was continued with a second treatment group (cohort) to assess the pharmacokinetic profile of formulation F7 of Example 7 compared with that of formulation F2.

Study Design and Duration of Treatment

[0339] Four single doses of the following tablet formulations were administered once per day orally during 36 days total study duration:

(a) a fixed dosed combination tablet containing emtricitabine and tenofovir alafenamide (200/25 mg - Tablet F3) and a single agent tablet containing the compound of Formula I (75 mg - Tablet Fl) administered simultaneously, under fasted conditions (Treatment A) (dosed on day l, days 2-8 washout);

(b) a fixed dose combination tablet containing the compound of Formula I, emtricitabine, and tenofovir alafenamide (50/200/25 mg - Tablet F2) administered under fasted conditions (Treatment D) (dosed on day 9; days 10-16 washout); and (b) a fixed dose combination tablet containing the compound of Formula I, emtricitabine, and tenofovir alafenamide (50/200/25 mg - Tablet F2) administered under fed conditions with a high-fat meal (Treatment E) (dosed on day 17, days 18-24 washout);

(c) a fixed dose combination tablet containing the compound of Formula I, emtricitabine, and tenofovir alafenamide (50/200/25 mg - Tablet F2) administered under fed conditions with a moderate-fat meal (Treatment F) (dosed on day 25; day 29 discharge).

Criteria for Evaluation

[0340| The following plasma pharmacokinetic parameters were calculated: AUChst, AUCinf, %AUC_cxp, Cmas? Clasb T_max, Ti_ast, CL/F, V_z/F, and ti/2.

Statistical Methods

[0341] Pharmacokinetics: Individual subject concentration data and individual subject PK parameters for each analyte (compound of Formula 1, FTC, and TAF) were listed and summarized using descriptive statistics by treatment group (cohort) and treatment. Summary statistics were determined for both individual subject concentration data by time point, cohort, and treatment and individual subject PK parameters by cohort and treatment. Also, the géométrie mean, 95% confidence interval (CI), and the mean, and standard déviation (SD) of the natural log-transformed values were presented for individual subject PK parameter data. The sample size for each time point was based on the number of subjects with nonmissing concentration data at that time point. The number of subjects with concentration below the level of quantitation (BLQ) was presented for each time point.

[0342] Statistical comparisons of the natural log-transformed PK parameters for each analyte and treatment comparison of interest were performed. The statistical modelling was based on the PK Analysis set for the analyte under évaluation. For each analyte, ail subjects with available data for the PK parameter under évaluation were included in the modelling. The statistical comparisons using Treatment A as a reference only used data from this cohort, not from the eariier cohort of Example 3 (i.e., Treatment A was not pooled).

For each analyte and each PK parameter, a paramétrée (normal theory) mixed-effects ANOVA model was fitted to the natural log-transformed values of the PK parameter under évaluation. The statistical model included treatment as a fixed effect and subject as a random effect.

[0343] A total of 27 subjects completed the second treatement group of the study.

Results

[0344] Plasma pharmacokinetic parameters for each analyte are presented below. This table présents the mean (%CV) values, Géométrie Least Squares Mean (GLSM) ratios, and 90% CI values of the plasma PK parameters ÀUCi_3St, AUCi„f, and C_TOv for each active ingrédient, following administration of bilayer tablet formulation F7 and monolayer tablet formulation Fl coadministered with F/TAF formulation F3, under fasted conditions.

	Mean (%CV)	%GLSM Ratio (90% CI) (T est/Refcrence)
Formulation F7 Compound of Formula 1/F/TAF (50/200/25 mg), fasted (Test) (N = 27)	Formulation Fl + F3 Compound of Formula (75 mg) + F/TAF (200/25 mg)), fasted (Reference) (N = 28)
Compound of Formula I PK Paramcter
AUCîasi (hr*ng/mL)	109,061.4(21.0)	142,396.6 (30.5)	78.46 (73.38,83.89)
AUCinr (hr-ng/mL)	112,619.6(21.9)	146,931.6(31.1)	78.56 (73.44, 84.04)
Cmax (ng/mL)	5228.1 (16.9)	6791.1 (26.4)	78.07(73.41,83.01)
FTC PK Parameter
AUCta (hr*ng/mL)	10,652.9(13.6)	11,035.5(14.4)	96.52(93.95, 99.15)
AUCinf (hr'ng/mL)	10,873.9(13.6)	11,234.6(14.2)	96.76 (94.22, 99.37)
Cmax (ng/mL)	2220.4 (30.1)	2166.4 (27.0)	102.36(93.85, 111.64)
TAF PK Parameter1
AUCiast (hfng/mL)	207.1 (46.5)	236.7 (45.3)	85.37 (75.24, 96.85)
AUCmf (hr*ng/mL)	208.8 (46.3)	238.3 (45.0)	85.48 (75.33, 97.00)
Cmax (ng/mL)	249.2 (51.6)	291.9(55.4)	84.17 (67.59, 104.81)

[0345] In particular, these data conftrmed that the exposure of the compound of Formula I in Formulation F7 was proportionally lower than that observed in Formulation F2 and were consistent with predicted exposures for the revised dose. These data were consistent with the modelling of earlier clinical data confirming that these exposures provided anti-HTV antiviral efficacy. FTC and TAF exposure were similar between Formulation F7 and Formulation F2 coadministered with F3; TAF Cmax was slightiy lower (by approximately 16%) for Formulation F7 than for the coadministration of F2 and F3

[0346] The effect of food on the pharmacokinetics of the compound of Formula I, emtricitabine and tenofovir alafenamide when administered in formulation F7 was also examined in a further treatment group of the above described clinical study.

[0347] The mean (%CV) values, GLSM ratios, and 90% CI values of the plasma for PK parameters AUCi_ast, AUCinf, and C_mJX for the compound of Formula I, emtricitabine, and tenofovir alafenamide following administration of tablet formulation F7 under fasted conditions or with a high-fat meal and with a moderate-fat meal are presented in the following table.

	Mean (%CV)	%GLSM Ratio (90% CI) (Test/Rcfcrence)
Compound of Formula I/F/TAF (50/200/25 mg) high fat meal (Test) (N = 27)	Compound of Formula I/F/TAF (50/200/25 mg) fasted (Reference) (N = 27)
Compound of Formula IPK Parameter
AUCiast (hr’ng/mL)	135,117.3(21.1)	109,061.4 (21.0)	123.96(115.91, 132.57)
AUCmf (hnng/mL)	140,032.4(21.8)	112,619.6 (21.9)	124.41 (116.27, 133.11)
Cmax (ng/mL)	5936.3 (18.3)	5228.1 (16.9)	113.23 ( 106.45, 120.43)
FTC PK Parameter
AUCiast (hr’ng/mL)	10,2)3.0(12.0)	10,652.9(13.6)	96.02 (93.47,98.65)
AUCinf (hr’ng/mL)	10,467.0 (11.9)	10,873,9(13.6)	96.41 (93.88, 99.02)
Cnmt (ng/mL)	1881.1 (24.2)	2220.4(30.1)	85.52(78.37,93.31)
TAF PK Parameter1*
AUCiast (hr’ng/mL)	310.3(34.9)	207.1 (46.5)	162.62(143.10, 184,80)
AUCinf (hr’ng/mL)	318.4(32.8)	208.8 (46.3)	166.55(146.54, 189.29)
Cmax (ng/mL)	236.6(65.1)	249.2(51.6)	91.71 (73.46, 114.49)
	Mean (%CV)	%GLSM Ratio (90% CI) (Test/Reference)
Compound of Formula I/F/TAF (50/200/25 mg) moderate fat meal (Test) (N = 27)	Compound of Formula I/F/TAF (50/200/25 mg) fasted (Reference) (N = 27)
Compound of Formula IPK Parameter
AUCiw (hr’ng/mL)	135,217.3(22.9)	109,061.4 (21.0)	123.56(115.53, 132.14)
AUCmf (hrng/mL)	140,197.7(23.6)	112,619.6 (21.9)	124.06 (115.95, 132.74)
Cmax (ng/mL)	6279.6(18.3)	5228.1 (16.9)	119.90 ( 112.72, 127.53)
F1C PK Parameter
AUCiast (hr’ng/mL)	10,738.3(9.8)	10,652.9 ( 13.6)	101.17(98.48, 103.94)
AUCmf (hr’ng/mL)	10,973.3(9.5)	10,873.9(13.6)	101.33(98.66,104.06)
Cm» (ng/mL)	1998.9(18.4)	2220.4(30.1)	91.84 (84.17,100.21)
TAF PK Parameter*
AUCiast (hr’ng/mL)	290 6 (41.3)	207.1 (46.5)	148.20(130.41, 168.41)
AUCmf (Iwng/mL)	293.1 (40.9)	208.8 (46.3)	148.23 ( 130.42, 168.47)
Cranx (ng/mL)	251.1 (66.7)	249.2(51.6)	99.04 (79.33, 123.65)
a N = 28 for tlie Reference group

[0348) Compared with administration under fasted conditions, administration ofthe tablet formulation F7 with a high-fat meal resuited m a 24% higher AUCinf and a 13% higher C_max for the compound of Formula I. Administration of a moderate fat meal, resuited in a 24% higher AUC_mf and a 20% higher

Cmax for the compound of Formula I. The impact of food on emtricitabine and tenofovir alafenamide exposure was similar to that previously observed.

[0349] These data confirm that a fixed dosed combination tablet formulation of the compound of Formula I, emtricitabine, and tenofovir alafenamide may be taken without regard to food.

Example 10 — Compression Studies

[0350] To further investigate the properties of the compositions, the following studies investigated blend compressibility and layer adhesion.

[0351 ] First, various final blend compositions including the Compound of Formula I (as a sodium sait,

i.e., the Compound of Formula H) were prepared with varying levels of intergranular or extragranular lactose is shown in the table below.

Compound of Formula Π: Final Powder Blends Containing Lactose

Components	Tablet Formulation
F9	F10	Fil	F12	F13	F14
Intergranular % w/w
Compound of Formula Π	16.24	16.24	16.24	16.24	14.86	14.86
Microcrystalline Cellulose	46.25	56.25	56.25	56.25	69.12	60.63
Lactose	20.00	NA	NA	NA	0.00	8.50
Croscarmellose Sodium	6.00	6.00	6.00	6.00	5.49	5.49
Magnésium Stearate	0.75	0.75	0.75	0.75	0.69	0.69
Extragranular % w/w
Microcrystallinc Cellulose	10.00	10.00	5.00	15.00	9.15	9.15
Lactose	NA	10.00	15.00	5.00	NA	NA
Magnésium Stearate	0.75	0.75	0.75	0.75	0.69	0.69
Layer weight (mg)	323	323	323	323	353	353

[0352] Next, final blend compositions of the Compound of Formula II with varying amounts of magnésium stéarate ranging from 0.75% to 0.5% were prepared as shown on the following table:

Compound of Formula I: Final Powder Blends with Varying Levels of Magnésium Stéarate

Components	Tablet Formulation
F15	F16	F17	F18	F19	F20

Intergranular %w/w

Compound of Formula Π	16.24	16.24	16.24	16.24	16.24	16.24
Microcrystalline Cellulose	66.25	66.30	66.40	66.40	66.50	66.75
Croscarmellose Sodium	6.00	6.00	6.00	6.00	6.00	6.00
Magnésium Stéarate	0.75	0.70	0.60	0.60	0.50	0.50
Extragranular °îw/w
Microcrystalline Cellulose	10.00	10.00	10.00	10.00	10.00	10.00
Magnésium Stéarate	0.75	0.75	0.75	0.75	0.75	0.5
Layer weight (mg)	323	323	323	323	323	323

[0353] Finally, final blend compositions containing emtricitabine and tenofovir alafenamide were prepared with varying amounts of magnésium stéarate ranging from 0.75% to 0.5% as shown in the table below:

Quantitative Compositions of F/TAF Final Powder Blends

Components	Tablet Formulation
F21	F22	F23	F24
Intergranular °ow/w
Tenofovir Alafenamide Hemifumarate	7.5	7.5	7.5	7.5
Emtricitabine	53.1	53.1	53.1	53.1
Microcrystalline Cellulose	29.9	30.4	30	30.2
Croscarmellose Sodium	8.0	8.0	8.0	8.0
Magnésium Stéarate	0.75	0.50	0.70	0.60

Extragranular % »>/»>

Magnésium Stéarate	0.75	0.50	0.70	0.60
Layer Weight (mg)	377	377	377	377

|0354| Altering magnésium stéarate content was studied to evaluate the impact on the compound of Formula I and emtricitabine / tenofovir alafenamide blend compressibility and layer adhesion. Formulations were also monitored for punch filming and sticking.

[0355] Fourteen combinations of the final powder blends described in the above tables were prepared as summarized in the table below. Critical tamp force was determined for ail fourteen combinations.

Compression Study Critical Layer 1 Tamp Force Results

Formulation Combination	Tablet Formulation Layer 1	Tablet Formulation Layer 2	Critical Tamp Force (kN)	Observation
A*	F2I	F15	1.0	Layers adhered
B·	F21	F9	<1.5	Délamination
C*	F21	F10	<1.5	Délamination
D*	F21	Fil	<1.5	Délamination
E*	F21	F12	<1.5	Délamination
F*	F21	F15	<1.5	Délamination
G*	F21	F14	<1.5	Délamination
H	F15	F21	1.5	Délamination
I	FI5	F22	2.0	Layers adhered
J	F16	F23	2.0	Layers adhered
K	F17	F24	1.5	Layers adhered
L	F18	F22	2.0	Layers adhered
M	F19	F22	2.5	Layers adhered, sticking
N	F20	F22	2.5	Layers adhered, sticking

* Note F/TAF as layer 1 and Compound of Formula 1 as layer 2

IG: Intergranular

EG: Extragranular

MgSt Magnésium Stéarate

[0356] Combination A represents layer order and composition used as baseline for comparison of other compositions. The tablet formulation of each layer corresponds to the corresponding layer of tablet formulation F7.One objective was to investigate whether it was possible to improve on the critical tamp force of 1.0 kN observed for Combination A.

|0357] Combinations B - G represent studies in which lactose was included in the layer including the Compound of Formula I to investigate evaluate layer adhesion. Combination G included 20% lactose in the intergranular portion, with none in the extragranular portion. The ratio of lactose to microcrystalline cellulose ranged from 1:1, 3:1, 1:3 in Combinations C, D, and E, respectively. Combination G included 20 mg lactose in the intergranular portion. Délamination was observed at ] .5kN for Combinations B - G. As a resuit, lower tamp forces were not further investigated. When layer order was reversed (Combination H), délamination did not occur at 1.0 kN but was observed at

1.5 kN using the layer compositions of Combination A.

[0358] In Combinations I - N, varying the amount of magnésium stéarate in each layer was investigated to détermine the effect on compressibility and layer adhesion. Intergranular magnésium stéarate was 0.75%, 0.70% and 0.60% in the Compound of Formula I layer of Combinations I, J, and K, respectively. Total magnésium stéarate content in the F/TAF layer of each of Combinations I, J, and K was 1.0%, 1.4% and 1.2%%, respectively. Intergranular magnésium stéarate was 0.6% and 0.5% in the Compound of Formula I layer of Combinations L and M, respectively (with 0.75% extragranular magnésium stéarate in each). Combination N has the lowest overall level of magnésium stéarate in both layers (1.0% in each layer).

[03591 It was found that reducing magnésium stéarate from 0.75% to 0.5% in each of the intergranular and extragranular portions the emtricitabine / tenofovir alafenamide layer increased critical tamp force to 2.0 kN (combination I). Critical tamp force could be further improved to 2.5 kN when magnésium stéarate was reduced to 0.5% extragranular and/or intergranular in the layer containing the Compound of Formula I (combinations M and N), However, despite gaining an increase in critical tamp force, decreasing magnésium stéarate to 0.5% in each component of the powder blend containing the Compound of Formula 1 had a négative effect on sticking. Intermediate changes in magnésium stéarate (Combinations J - L) had no further improvement over combination I. As a resuit, magnésium stéarate level was decreased in the emtricitabine/tenofovir alafenamide layer only (formulation combination I). A critical layer 1 tamp force of 1377 N was ultimately determined. The table below summarizes the composition of Formulation I.

[0360] Thus, the addition of intergranular or extragranular lactose to the layer containing the Compound of Formula I had little to ono effect on layer adhesion. Decreasing magnésium stéarate from each of final powder blends improved compressibility.

100

Quantitative Composition of Combination Formulation I
Components	Quantity per Tablet (mg)	w/w (%)
Compound of Formula I Layer
Compound of Formula IF	52.5“·”	7.5
Microcrystalline Cellulose	246.3	35.2
Croscarmellose Sodium	19.4	2.8
Magnésium Stéarate	4.9	0.7
Subtotal	323	46.1
F/TAF Layer
Emtricitabine'	200.0e	28.6
Tenofovir Alafenamide Fumarate'	28.0e-d	4.0
Microcrystaliine Cellulose	115.0	16.4
Croscarmellose Sodium	30.2	4.3
Magnésium Stéarate	3.8	0.5
Subtotal	377	53.9
Total Bilayer Tablet Core Weight	700	100
Film Coat
Opadry'II Brown 85F165072c	21'	3f
Purified Waterf	___g	___s

a The quantity used is adjusted on the basis of the purity of each batch of Compound of Formula II with a concomitant adjustment of the quantity of microcrystalline cellulose.

b Equivalent to 50 mg of Compound of Formula l free acid.

c The quantity used is adjusted on the basis of the purity of each batch of emtricitabine and tenofovir alafenamide hemifumarate with a concomitant adjustment of the quantity of microcrystalline cellulose.

d Equivalent to 25 mg of tenofovir alafenamide free base.

e Opadry II Brown 85F165072 contains polyvinyl alcohol, USP (40.0% w/w); titanium dioxidc, USP (22.0% w/w); polyethylene glycol, NF (20.2% w/w); talc, USP (14.8% w/w); iron oxide red, NF (2.4% w/w); iron oxide black, NF (0.6% w/w).

f Represents a theoretical weight gain of 3% w/w (range 2% to 4% w/w) onto the tablet core weight.

g Sufficient purified water is used for film coating and is removed during the process |0361] Ail publications, patents and patent applications are incorporated by reference in their entirety, as though individually incorporated by reference. The invention has been described with reference to various spécifie and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention.

Claims (9)

1. Claims:

   l. A tablet comprising 50 mg of the compound of Formula I:

   (D

   or a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide or a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine or a pharmaceutically acceptable sait thereof.
2. 2. The tablet of claim l, wherein the tablet comprises 50 mg of the compound of Formula I as a pharmaceutically acceptable sait thereof, 25 mg tenofovir alafenamide as a pharmaceutically acceptable sait thereof, and 200 mg emtricitabine.
3. 3. The tablet of claim l or 2, wherein the tablet comprises 52 mg of the sodium sait of the compound of Formula I and 28 mg tenofovir alafenamide hemifumarate.
4. 4. The tablet o f any one of the preceding claims, wherein the tablet has a total weight of:

   (i) less than about l .0 g;

   (ii) less than about 800 mg; or (iii) less than about 730 mg.
5. 5. The tablet of any one of the preceding claims, wherein the tablet is a coated tablet.
6. 6. The tablet of any one of the preceding claims, wherein the tablet contains less than about 15% ofthe compound of Formula I or a pharmaceutically acceptable sait thereof.
7. 7. The tablet of any oneof the preceding claims, wherein the tablet contains less than about 11% of the compound of Formula I or a pharmaceutically acceptable sait thereof.
8. 8. The tablet ofany one ofthe preceding claims comprising:

   (i) a first layer including:

   102

   Ingrédient Mass (mg/ta blet) Compound of Formula II 52.5 (équivalent to 50 mg of the Compound of Formula I) Microcrystalline cellulose 246.3 Croscarmellose sodium 19.4 Magnésium Stearate 4.9

   (ii) a second layer including:

   Ingrédient Mass (m g/ta blet) Emtricitabine 200 Tenofovir alafenamide hemifumarate 28 (équivalent to 25 mg tenofovir alafenamide) Microcrystalline cellulose 113.2 Croscarmellose sodium 30.2 Magnésium Stearate 5.7

   and (iii) a film coating.
9. 9. A tablet according to any one of the preceding claims for use in the therapeutic treatment of an HIV infection.