OA16572A - Smac mimetic. - Google Patents

Abstract

A smac mimetic and pharmaceutical compositions thereof and methods of use.

Description

This invention is in the field of SMAC mimetics and compositions and uses thereof to treat proliférative disorders including cancers.

Background of the Invention

Inhibitors of Apoptosis Proteins (lAPs) are naturally occurring intra-cellular proteins that suppress caspase-dependent apoptosis. SMAC, also known as DIABLO, is another intracellular protein that functions to antagonize, i.e., inhibit the activity of lAPs. In normal healthy cells, SMAC and lAPs function together to maintain healthy cells. However, in certain disease states, e.g., cancers and other proliférative disorders, lAPs are not adequately antagonized and therefore prevent apoptosis and cause or exacerbate abnormal prolifération and survival.

SMAC mimetics, also known as IAP antagoniste, are synthetic small molécules that mimic the structure and IAP antagonist activity of the four N-terminal amino acids of SMAC. (SMAC mimetics are sometimes referred to as IAP antagonists.) When administered to animais suffering proliférative disorders, the SMAC mimetics antagonize lAPs, causing an increase in apoptosis among abnormally proliferating cells.

Examples of SMAC peptidomimetics are those disclosed in US 7,517,906; US 7,309,792; US 7,419,975; US 2005/0234042; US 2005/0261203; US 2006/0014700; US 2006/0025347; US 2006/0052311; US 2006/0128632; US 2006/0167066; US 2007/0042428; US 2007/032437; US 2008/0132485; WO 2005/069888; WO 2005/069894; WO 2006/010118; WO 2006/122408; WO 2006/017295; WO 2006/133147; WO 2006/128455; WO 2006/091972; WO 2006/020060; WO 2006/014361; WO 2006/097791; WO 2005/094818; WO 2008/045905; WO 2008/016893; WO 2007/136921; WO 2007/021825; WO 2007/130626; WO 2007/106192; and WO 2007/101347.

Summary of the Invention

This invention, in one aspect, is N-{1S-[2R-(6,6'-Difluoro-3'-{4S-hydroxy-1-[2S-(2S-methylaminopropionylamino)-butyryl]-pyrrolidin-2R-ylmethyl}-1H,1'H-[2,2’]biindolyl-3-ylmethyl)-4S-hydroxypyrrolidine-1-carbonyl]-propyl}-2S-methylamino-propionamide and pharmaceutically acceptable salts thereof, as well as various forms of such compound and salts thereof as further described herein below.

This compound has the following structure:

wherein R5 is -CH2CH3. This compound is also referred to herein as Compound 15.

The invention, in related aspects, comprises a pharmaceutical composition comprising such compound and a method of treating a proliférative disorder in a human or non-human mammalian subject in need thereof that comprises internally administering to the subject an effective amount of said compound or a pharmaceutically acceptable sait thereof.

In other aspects, the invention comprises a method of treating a proliférative disorder in a mammal in need thereof, e.g., a human, or a companion animal, a food animal, or a sporting animal, that comprises internally administering to the animal an effective amount of Compound 15 or a pharmaceutically acceptable sait thereof.

In another illustrative embodiment, the invention comprises a method for inducing apoptosis in a cell comprising contacting the cell with Compound 15 or a pharmaceutically acceptable sait thereof. In this embodiment, the cell can be, e.g., a cancerous cell.

In additional illustrative embodiments, the invention comprises any one or more ofthe above methods that further comprises administering a second cancer-related therapy, such as, e.g., radiation, chemotherapy, immunotherapy, photodynamic therapy, and combinations thereof.

In a further illustrative embodiment, the invention comprises a method of treating an autoimmune disease, in which the condition is caused or exacerbated by abnormal régulation of apoptosis, in a mammal in need thereof, including, for example, systemic lupus erythematosus, psoriasis, and idiopathic thrombocytopénie purpura (Morbus Werlhof) that comprises internally administering to the animal an effective amount of Compound 15 or a pharmaceutically acceptable sait thereof.

Brief Description ofthe Figures

Figure 1 shows percent mean body weight loss in rat following 4 days of intravenous bolus dosing with SMAC mimetics substantially as described in Example 4.

Figure 2 shows mean tumor volume (2A) and body weight change (2B) resulting from treatment of human xenografts in nude mice with SMAC mimetics substantially as described in Example

5.

Detailed Description of the Invention

The compound of the invention is a SMAC mimetic that can be used in the treatment of proliférative disorders, e.g.: various benign tumors or malignant tumors (cancer), benign proliférative diseases (e.g., psoriasis, benign prostatic hypertrophy, and restenosîs), or autoimmune diseases (e.g., autoimmune proliférative glomerulonephritis, lymphoproliférative autoimmune responses). Cancers which potentially can be treated with IAP antagonists include, but are not limited to, one or more of the following: lung adenocarcinoma, pancreatic cancer, colon cancer, ovarian cancer, breast cancer, mesothelioma, peripheral neuroma, bladder cancer, glioblastoma, melanoma, adrenocortical carcinoma, AIDS-related lymphoma, anal cancer, bladder cancer, meningioma, glioma, astrocytoma, breast cancer, cervical cancer, chronic myeloprolîferative disorders (e.g., chronic lymphocytic leukemia, chronic myelogenous leukemia), colon cancer, endocrine cancers, endométrial cancer, ependymoma, esophageal cancer, Ewing's sarcoma, extracranial germ cell tumors, extragonadal germ cell tumors, extrahepatic bile duct cancer, gallbladder cancer, gastric cancer, gastrointestinal carcinoid tumors, gestational trophoblastic tumors, hairy cell leukemia, Hodgkin lymphoma, non-Hodgkin lymphoma, hypopharyngeal cancer, intraocular melanoma, islet cell carcinoma, Kaposi sarcoma, laryngeal cancer, leukemia, acute lymphoblastic leukemia, acute myeloid leukemia, lip cancer, oral cavity cancer, liver cancer, male breast cancer, malignant mesothelioma, medulloblastoma, melanoma, Merkel cell carcinoma, metastatic squamous neck cancer, multiple myeloma and other plasma cell neoplasms, mycosîs fungoides and the Sezary syndrome, myelodysplastic syndromes, nasopharyngeal cancer, neuroblastoma, non-small cell lung cancer, small cell lung cancer, oropharyngeal cancer, bone cancers, including osteosarcoma and malignant fibrous histiocytoma of bone, ovarian épithélial cancer, ovarian germ cell tumors, ovarian low malignant potential tumors, pancreatic cancer, paranasal sinus cancer, parathyroid cancer, penile cancer, pheochromocytoma, pïtuitary tumors, prostate cancer, rectal cancer, rénal cell cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, skin cancer, small intestine cancer, soft tissue sarcoma, supratentorial primitive neuroectodermal tumors, pineoblastoma, testicular cancer, thymoma, thymie carcinoma, thyroid cancer, transitional cell cancer of the rénal pelvis and ureter, uréthral cancer, uterine sarcoma, vaginal cancer, vulvar cancer, and Wilm's tumor and other childhood kidney tumors.

Some embodiments of the invention include inducing apoptosis of cells, particularly pathologically proliferating cells. The methods can be carried out in vitro or in vivo.

The methods of the invention can include administration of the compound of the invention alone, administration of a combination of IAP antagonists, or administration of the compound of the invention, with or without one or more additional IAP antagonists, and one or more additional chemotherapeutic agents. Administration of multiple agents can be simultaneous or sequential. Useful chemotherapeutic agents include, but are not limited to, alkylating agents (e.g., cyclophosphamide, mechlorethamine, chlorambucil, melphalan), anthracyclines (e.g., daunorubicin, doxorubicin, epirubicin, idarubicin, mitoxantrone, valrubicin), cytoskeletal disruptors (e.g., paclitaxel, docetaxel), epothilones (e.g., epothilone A, epothilone B, epothîlone D), inhibitors of topoisomerase II (e.g., etoposide, teniposide, tafluposide), nucléotide analogs precursor analogs (e.g., azacitidine, azathioprine, capecitabine, cytarabine, doxifluridine, fluorouracil, gemcitabine, mercaptopurine, methotrexate, tioguanine), peptide antibiotics (e.g., bleomycin), platinum-based agents (e.g., carboplatin, cisplatin, oxaliplatîn), retinoids (e.g., alltrans retinoic acid), and vinca alkaloids and dérivatives (e.g., Vinblastine, vincristine, vindesine, vinorelbine). In some embodiments, chemotherapeutic agents include fludarabine, doxorubicin, paclitaxel, docetaxel, camptothecin, etoposide, topotecan, irinotecan, cisplatin, carboplatin, oxaliplatîn, amsacrine, mitoxantrone, 5-fluoro-uracil, or gemcitabine.

In some embodiments ofthe invention, pharmaceutical compositions comprising the compound of the invention, alone or in combination with one or more other active pharmaceutical ingrédients, are administered to a human or veterinary subject. The pharmaceutical compositions typically comprise at least one pharmaceutically acceptable excipient, e.g., a carrier or diluent, and can be administered in the conventional manner by routes including systemic, topical, or oral routes. Administration is normally by intravenous injection, either as a bolus or infusion, but other routes of administration are not precluded. An intravenous formulation can be 1 mg/mL of Compound 15 in stérile 0.05M citrate buffered PBS, pH5. Spécifie modes of administration will dépend on the indication and other factors including the particular compound being administered. The amount of compound to be administered is that amount which is therapeutically effective. The dosage to be administered will dépend on the characteristics ofthe subject being treated, e.g., the particular patienttreated, age, weight, health, types of concurrent treatment, if any. Frequency of treatments can be easily determined by one of skill in the art (e.g., by the clinîcian).

Typically, the compound ofthe invention will be administered by intravenous injection, including, e.g., by infusion over about 1 to about 120 minutes, e.g., about 30 minutes.

The pharmaceutical composition of the invention is a composition in which the active pharmaceutical ingrédient, i.e., the compound ofthe invention, is pure enough, and the composition is otherwise suitable, for internai administration to a human or other mammal. It can be prepared in unit dose form, i.e., a form suitable for single administration to a subject. So, e.g., a pharmaceutical composition in intra-venous unit dose form may comprise a vial or prefilled syringe, each comprising an effective amount or a convenient fraction of an effective amount such that one the contents of one vial or syringe are administered at a time. Such administration can be repeated up to about 4 times per day over a period of time, if necessary to achieve a cumulative effective dose, e.g., tumor régression. A dosing regimen can be, e.g., daily or twice-weekly intravenous injections, or, e.g., weekly injections in cycles of three weeks on and one week off for as long as the treatment is effective, e.g., until disease progresses or the drug is not tolerated. The effective dose administered in each injection is an amount that is effective and tolerated; it can be, e.g., 0.01 to 30 mg/m², e.g., 0.2 to 10 mg/m², or, e.g., 0.5 to 5 mg/m².

The compound ofthe invention can also be applied locally, such as in isolated limb perfusion. The compound ofthe invention can also be applied topically, e.g., as a cream, gel, lotion, or ointment, or in a réservoir or matrix-type patch, or in an active transdermal delivery system. An effective dose is one that over the course of therapy, which may be, e.g., 1 or more weeks, e.g., multiple courses of 3 weeks on/1 week off, results in treatment ofthe proliférative disorder,

i.e., a decrease in the rate of disease progression, termination of disease progression, or régression or rémission.

Pharmaceutical compositions to be used comprise a therapeutically effective amount of a compound as described above, or a pharmaceutically acceptable sait or other form thereof together with one or more pharmaceutically acceptable excipients. The phrase pharmaceutical composition refers to a composition suitable for administration in medical use. It should be appreciated that the déterminations of proper dosage forms, dosage amounts, and routes of administration for a particular patient are within the level of ordinary skill in the pharmaceutical and medical arts.

Compositions suitable for parentéral administration conveniently comprise a stérile aqueous préparation of the compound of the invention, which is preferabiy isotonie with the blood of the récipient. This aqueous préparation may be formulated according to known methods using suitable carriers or diluents which may include a buffer.

When practicing the conjoint or combination therapy described in more detail below, the administration of the compound and compositions of the présent invention can occur simultaneous with, subséquent to, or prior to chemotherapy or radiation, so long as the chemotherapeutic agent or radiation sensitizes the system to the compound and compositions of the présent invention.

The présent invention also is directed to the use of the compound and compositions as a chemopotentiating agent with other treatment approaches. The term chemopotentiating agent refers to an agent that acts to increase the sensitivity of an organism, tissue, or cell to a chemical compound, or treatment namely chemotherapeutic agents or chemo drugs or to radiation treatment. Thus, compound and compositions of the présent invention can be used for inhibiting tumor growth in vivo by administering them in combination with a biologie or chemotherapeutic agent or by using them in combination with radiation. In these applications, the administration of the compound and compositions of the présent invention may occur prior to, and with sufficient time, to cause sensitization ofthe site to be treated. Alternatively, the compound and compositions of the présent invention may be used contemporaneously with radiation and/or additional anti-cancer chemical agents (infra). Such Systems can avoid repeated administrations of the compound and compositions of the présent invention, increasing convenience to the subject and the physician, and may be particularly suitable for certain compositions of the présent invention.

Biological and chemotherapeutics/anti-neoplastic agents and radiation induce apoptosis by activating the extrinsic or intrinsic apoptotic pathways, and, since the compound and compositions of the présent invention relieve antagonists of apoptotic proteins (lAPs) and, thus, remove the block in apoptosis, the combination of chemotherapeutics/anti-neoplastic agents and radiation with the compound and compositions of the présent invention should work additively or synergistically to facilitate apoptosis.

A combination of the compound ofthe présent invention and a biological or chemotherapeutic/anti neoplastic agent and/or radiation therapy of any type that activâtes the extrinsic or intrinsic pathway may provide a more effective approach to destroying tumor cells. The compound of the présent invention interacts with lAP's, such as XIAP, clAP-1, clAP-2, MLIAP, etc., and removes the IAP mediated block of apoptosis. Most chemotherapeutics/anti neoplastic agents and/or radiation therapy kills actively dividing cells by activating the intrinsic apoptotic pathway leading to apoptosis and cell death. Biological antitumor agents such as TRAIL (TNF-related apoptosis inducing ligand) activate extrinsic apoptotic pathways. As is described in more detail below, embodiments of the invention provide combinations of the compound of the présent invention and a biological or chemotherapeutic/anti-neoplastïc agent and/or radiation which provide a synergistic action against unwanted cell prolifération. This synergistic action between the compound ofthe présent invention and a biological or chemotherapeutic/anti-neoplastic agent and/or radiation therapy can improve the efficiency of the biological or chemotherapeutic/anti-neoplastic agent and/or radiation thérapies. This will allow for an increase in the effectiveness of current biological or chemotherapeutic/antineoplastic agents or radiation treatments allowing a higher percentage of tumors to respond to the therapy, an improved tumor response, and, potentially, a réduction in the dose of the biological or chemotherapeutic/anti-neoplastic agent needed to treat a tumor, thereby providing the use of a more tolerable dose of biological or chemotherapeutic/anti-neoplastic agent and/or radiation.

In an embodiment of the présent invention, the patient is treated by administering the compound or a pharmaceutical composition of the présent invention at a time the patient is subject to concurrent or antécédent radiation or chemotherapy for treatment of a neoproliferative pathology of a tumor such as, but not limited to, bladder cancer, breast cancer, prostate cancer, lung cancer, pancreatic cancer, gastric cancer, colon cancer, ovarian cancer, rénal cancer, hepatoma, melanoma, lymphoma, sarcoma, and combinations thereof.

In another embodiment ofthe présent invention, the compound or a composition ofthe présent invention can be administered in combination with a biological or chemotherapeutic and/or for use in combination with radiotherapy, immunotherapy, and/or photodynamrc therapy, promoting apoptosis and enhancing the effectiveness of the chemotherapeutic, radiotherapy, immunotherapy, and/or photodynamic therapy.

Embodiments of the invention also include a method of treating a patient afflicted with cancer by the contemporaneous or concurrent administration of a biological or chemotherapeutic agent. Such biological or chemotherapeutic agents include but are not limited to the chemotherapeutic agents described in Modem Pharmacology with Clinical Applications, Sixth Edition, Craig & Stitzel, Chpt. 56, pg 639-656 (2004), herein incorporated by reference. The chemotherapeutic agent can be, but is not limited to, alkylating agents, antimetabolites, anti-tumor antibiotics, plant-derived products such as taxanes, enzymes, hormonal agents, miscellaneous agents such as cisplatin, monoclonal antibodies, glucocorticoids, mitotic inhibitors, topoisomerase I inhibitors, topoisomerase II inhibitors, immunomodulating agents such as interferons, cellular growth factors, cytokines, and nonsteroidal anti-inflammatory compounds (NSAID), cellular growth factors and kinase inhibitors. Other suitable classifications for chemotherapeutic agents include mitotic inhibitors,and anti-estrogenic agents.

Spécifie examples of suitable biological and chemotherapeutic agents include, but are not limited to, cisplatin, carmustine (BCNU), 5-fluorouracil (5-FU), cytarabine (Ara-C), gemcitabine, methotrexate, daunorubicin, doxorubicin, dexamethasone, topotecan, etoposide, paclitaxel, vincristine, tamoxlfen, TNF-alpha, TRAIL and other members, i.e., other than TRAIL and TNFalpha, of the TNF superfamily of molécules., interferon (in both its alpha and beta forms), thalidomide, thalidomide dérivatives such as lenalidomide, melphalan, and PARP inhibitors. Other spécifie examples of suitable chemotherapeutic agents include nitrogen mustards such as cyclophosphamide, alkyl sulfonates, nitrosoureas, ethylenîmines, triazenes, folate antagonists, purine analogs, pyrimidine analogs, anthracyclines, bleomycins, mitomycins, dactinomycins, plicamycin, vinca alkaloids, epipodophyllotoxins, taxanes, glucocorticoids, L-asparaginase, estrogens, androgens, progestins, luteinizing hormones, octreotide actetate, hydroxyurea, procarbazine, mitotane, hexamethylmelamine, carboplatin, mitoxantrone, monoclonal antibodies, levamisole, interferons, interleukins, filgrastim and sargramostim.

Another embodiment of the présent invention relates to the use of the compound or a composition of the présent invention in combination with topoisomerase inhibitors to potentiate their apoptotic inducing effect. Topoisomerase inhibitors inhibit DNA réplication and repair, thereby promoting apoptosis and are used as chemothemotherapeutic agents. Topoisomerase inhibitors promote DNA damage by inhibiting the enzymes that are required in the DNA repair process. Therefore, export of Smac from the mitochondria into the cell cytosol is provoked by the DNA damage caused by topoisomerase inhibitors. Topoisomerase inhibitors of both the Type I class (camptothecin, topotecan, SN-38 (irinotecan active métabolite) and the Type II class (etoposide) are expected to show potent synergy with compounds of the présent invention. Further examples of topoisomerase inhibiting agents that may be used include, but

O are not limited to, irinotecan, topotecan, etoposide, amsacrine, exatecan, gimatecan, etc. Other topoisomerase inhibitors include, for example, Aclacinomycin A, camptothecin, daunorubicin, doxorubicin, ellipticine, epirubicin, and mitaxantrone.

Another embodiment of the présent invention relates to the use of the compound or a composition of the présent invention in combination with nonsteroidal antiinflammatory drugs (NSAIDs).

ln another embodiment of the invention, the chemotherapeutic/anti-neoplastic agent for use in combination with the compound and compositions of the présent invention may be a platinum containing compound. ln one embodiment ofthe invention, the platinum containing compound is cisplatin. Cisplatin can synergize with a compound of the présent invention and potentiate the inhibition of an IAP, such as but not limited to XIAP, clAP-1, c-IAP-2, ML-IAP, etc. ln another embodiment a platinum containing compound is carboplatin. Carboplatin can synergize with a compound ofthe présent invention and potentiate the inhibition of an IAP, including, but not limited to, XIAP, clAP-1, c-IAP-2, ML-IAP, etc. ln another embodiment a platinum containing compound is oxaliplatin. The oxaliplatin can synergize with a compound of the présent invention and potentiate the inhibition of an IAP, including, but not limited to, XIAP, clAP-1, cIAP-2, ML-IAP, etc.

Platinum chemotherapy drugs belong to a general group of DNA modifying agents. DNA modifyîng agents may be any highly reactive chemical compound that bonds with various nucleophilic groups in nucleic acids and proteins and cause mutagenic, carcinogenic, or cytotoxic effects. DNA modifying agents work by different mechanisms, disruption of DNA function and cell death; DNA damage/the formation of cross-bridges or bonds between atoms in the DNA; and induction of mispairing ofthe nucléotides leading to mutations, to achieve the same end resuit. Three non-limiting examples of a platinum containing DNA modifying agents are cisplatin, carboplatin and oxaliplatin.

Yet another embodiment of the présent invention is the therapeutic combination or the therapeutic use in combination of the compound or compositions of the présent invention with TRAIL orTRAIL agonist antibodies, or other chemical or biological agents which bind to and activate the TRAIL receptor(s). Many cancer cell types are sensitive to TRAIL-induced apoptosis, while most normal cells appear to be résistant to this action of TRAIL. TRAILresistant cells may arise by a variety of different mechanisms including loss of the receptor, presence of decoy receptors, overexpression of FLIP which competes for zymogen caspase-8 binding during DISC formation and inhibition of activated caspase-3 and/or caspase-9 by XIAP. ln TRAIL résistance, a compound or composition ofthe présent invention may increase tumor cell sensitivity to TRAIL leading to enhanced cell death, the clinical corrélations of which are expected to be increased apoptotic activity in TRAIL résistant tumors, improved clinical response, increased response duration, and ultimately, enhanced patient survival rate.

In another embodiment of the invention, Compound 15 is administered in combination with a cytokine, e.g., TNFa.

The compound and compositions of the présent invention also can be used to augment radiation therapy (or radiotherapy), i.e., the medical use of ionizing radiation as part of cancer treatment to control malignant cells. Although radiotherapy is often used as part of curative therapy, it is occasionally used as a palliative treatment, where cure is not possible and the aim is for symptomatic relief. Radiotherapy is commonly used for the treatment of tumors. It may be used as the primary therapy. It is also common to combine radiotherapy with surgery and/or chemotherapy. The most common tumors treated with radiotherapy are breast cancer, prostate cancer, rectal cancer, head & neck cancers, gynecological tumors, bladder cancer and lymphoma. Radiation therapy is commonly applied just to the localized area involved with the tumor. Often the radiation fields also include the draining lymph nodes. It is possible but uncommon to give radiotherapy to the whole body, or entire skin surface. Radiation therapy is usually given daily for up to 35-38 fractions (a daily dose is a fraction). These small frequent doses allow healthy cells time to grow back, repairing damage inflicted by the radiation. Three main divisions of radiotherapy are external beam radiotherapy or teletherapy, brachytherapy or sealed source radiotherapy and unsealed source radiotherapy, which are ail suitable examples of treatment protocol in the présent invention. The différences relate to the position of the radiation source; external is outside the body, while sealed and unsealed source radiotherapy has radioactive material delivered internally. Brachytherapy sealed sources are usually extracted later, while unsealed sources are injected into the body.

Compound 15 is capable of forming pharmaceutically acceptable salts, including but not limited to acid addition and/or base addition salts. Such salts are included within ail aspects of the invention.

It is intended that the présent invention encompass Compound 15 synthesized in vitro using laboratory techniques, such as those well known to synthetic chemists; or synthesized using in vivo techniques, such as through metabolism, fermentation, digestion, and the like. It is also contemplated that the compound of the présent invention may be synthesized using a combination of in vitro and in vivo techniques.

The présent invention also includes isotopically-enriched compounds, which are identical to Compound 15 but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be included in the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as ^aH, ³H, ¹³C, ¹⁴C, ^1SN, ¹⁶O, ¹⁷0,³¹P, ³²P, ³⁵S, ¹⁸F, and ³⁸CI. Substitution with heavier isotopes such as deuterium, i.e., ²H, are also included. Isotopically enriched compounds of this invention can generally be prepared by substituting a readily available isotopically labelled reagent for a non-isotopically enriched reagent. For example, incorporation of deuterium can be accomplished by substituting sodium borohydride with cM-sodium borohydride, or by replacing iodomethane with d3-iodomethane. Représentative examples of spécifie deuterated analogs and their préparation are described in Example 1.

Compound 15 may exist in unsolvated forms as well as solvated forms, încluding hydrated forms. Furthermore, Compound 15 may exist in various solid states încluding crystalline, semicrystalline and amorphous (noncrystalline) forms, and in the form of clathrates, prodrugs, polymorphs, bio-hydrolyzable esters, racemic mixtures, non-racemic mixtures, or as purified stereoisomers încluding, but not limited to, optically pure enantiomers and diastereomers. In general, ali of these and other such forms are intended to be encompassed within the scope of the term, Compound 15.

Référencés to Compound 15 and to the compound of the invention, and other similar phrases in this spécification and in the clairns, are intended to include not only the compound of formula (I), but also pharmaceutically acceptable salts of Compound 15, as well as various forms of said compound or salts thereof such as those that are described above and below.

In additional embodiments, the invention comprises compounds useful as intermediates in the synthesis of Compound 15, as well as in processes for preparing such intermediates and Compound 15. For example, in such embodiments, the invention comprises compounds shown in the Examples, below, such as Compounds 9, 10, 11, 12, 13, 14, and the isotopically enriched compounds such as Compounds 18 through 32. One such embodiment is Compound 15 in which the 4-OH substituent on the pyrrolidine moiety is protected with a protecting group. An illustrative protecting group is an acetyl group, which is illustrated in Compounds 11-14, below. Other useful protecting groups will be apparent to persons of skill in the art and include, e.g., benzoyl, benzyl, trimethylsilyl, and triphenylmethyl groups. The protecting group is removed, e.g., by contacting the protected intermediate with an acid or a base, as shown in Schemes XIII and XIV, below. Thus, the invention comprises the compound having the structure of Compound 15 as well as protected versions of Compound 15 such as Compounds 13 and 14 in which the N-termîni are protected with carbamate moieties and/or free hydroxyl groups are protected as esters such compounds being herein referred to as Protected Compound 15. The invention further comprises the step of deprotecting a Protected Compound 15 by contacting the Protected Compound 15 with an acid or base whereby the Protecting Group is removed to afford Compound 15. Isotopically enriched compounds of the invention include deuterated forms of Compound 15 such as Compounds 20, 29, and 32. Protected forms of such compounds, e.g., Compounds 19, 28, and 31, are also comprised within the invention.

Examples

The following préparations and schemes are illustrative of synthesis of compounds of the présent invention. Abbreviations which are used throughout these schemes and in the application generally, are identified in the following table:

ABBREVIATION

MEANING

ABBREVIATION

MEANING

ACN	Acetonitrile	NMP	N-methylpyrrolidinone
Ac2O	Acetic anhydride	PhCOCI	Benzoyl chloride
Cbz and Z	Benzyloxycarbonyl	DIAD	diisopropyl azo dicarboxylate
Boc and/or boc	tert-butyloxycarbonyl	DIBAL	Diisobutylaluminium hydride
THF	Tetrahydrofuran	DMAP	4-dimethylamino pyridine
DCM	Dichloromethane	DMF	Dimethylformamide
DDQ	2,3-dichloro-5,6-dicyano-1 ,4benzoquinone	DMSO	dimethyl sulfoxide
mCPBA	3-chloroperbenzoic acid	TFA	trifluoroacetic acid
Cbz-CI	Benzyloxycarbonyl chloride	TFAA	trifluoroactic anhydride
Hex	Hexanes	HOAc or AcOH	acetic acid
HPLC	high performance liquid chromatography	DIPEA	Diisopropylethylamine
TLC	thin layer chromatography	NMM	N-methylmorpholine
EtOAc	ethyl acetate	NCS	/V-chlorosuccinimide
Ph	Phenyl	TEA (Et3N)	Triethylamine
HATU	2-(7-Aza-1 H-benzotriazole-1-yl)1,1,3,3-tetramethyluronium hexafluorophosphate	MsCI	Methanesulfonylchloride
Me	Methyl*	Et	Ethyl
/Pr	Iso-propyl	tBu or tert-Bu	tert-butyl
cPr	Cyclopropyl	cHex	Cyclohexy)
(2R-EtOMe) and/or R-MeCHOMe	Me Mb — 'VuV	(2R-EtOH) and/or R-MeCHOH	Ma ^OH T
TBAF	tetrabutyl ammonium fluoride	MsCI	Methanesulfonyl chloride
OMs	Methanesulfonyloxy	OTs	-O-SOa-Ph-Me
TBDMSCI or TBSCI	tert-butyl-dimethyl-silyl chloride	OTBS	tert-butyl-dimethylsilanyloxy
Ph3P	Triphenylphosphine	Ac	0 Acetyl ( ° Me )
n-Bu	Normal butyl	DMA	Dimethylamine

?

Swern[O]	Swern Oxidation	HWE	Honer-WadsworthEmmons reaction
TBA-CI	Tetra-n-butyl ammonium chloride	DMS	Dîmethylsulfide
NP-HPLC	Normal phase-high performance liquid chromatography	Meldrum’s Acid	2,2-djmethyl-1,3dîoxane-4,6-dione
EDCI	N-3-(dimethylaminopropyl)-N'ethylcarbodiimide hydrochloride 1-Ethyl-3-(3- Dimethylaminopropyl)carbodiimideHCI	Imid.	Imidazole
Et2O	Ethylene oxide (^)	HOBT, or HBT	Hydroxybenzotriazole
TES	Triethylsilane	RT	Room température
MeNO2	Nitromethane	MeOH	Methanol
EtOH	Ethanol	NaOAc	Sodium acetate
DCE, or EDC	Dichloroethane, Ethylenedichloride	CICO2Me	Ethyl chloroformate
NaHMDS	Sodium hexamethyldisilazide or sodium bis(tnmethylsilyl)amide	TBSCI	tert-butyl-dimethylsilanyl chloride
Boc-Chg-OH (Boc-Lcyclohexylglcine)	0	Cbz-N(Me)AlaOH Z-N(Me)Ala-OH	(TA r 0 ο &·
Boc-N(Me)AlaOH	Mo O	Boc-Tle-OH	0 Ύ 0
Boc-Abu-OH	0	Boc-Val-OH	AÂXf
Boc-Ser-OH	-OH	Cbz-Ser(tBu)OH	C^t
Boc-Ser(Me)-OH	-OMû	Cbz-Thr(tBu)-OH	, A

916572

Boc-Thr(tBu)-OH		Boc-Thr-OH	aYÇ» 0
Boc-Thr(Me)-OH	0	PSI	Pounds per Square Inch (Gauge)
h	hour	NaOMe	Sodium methoxide

Example 1 - Synthesis

Scheme I

2

4-(tert-Butvl-dimethvl-silanyloxv)-pvrrolidine-1,2-dicarboxylic acid 1-benzyl ester (2): A solution of Z-Hyp-OH (1, 300 g, 1.13 mol), TEA (395 mL, 2.83 mol), and DBU (17.2 g, 1.13 mol) in DMF (1,25 L) was stirred in a cold water bath while a suspension of TBS-CI (188 g, 1.24 mol) in DMF (270 mL) was added slowly at 21-26 °C [Note: moderately exothermic]. The resulting thin suspension was stirred for 22 h at ambient température. The reaction mixture was cooled to 2 °C and quenched with water (1.54 L) at £ 26 °C [Note: the pH of the aqueous layer was 8.5-9.0], MTBE (3 L) was added and the mixture was acidified to pH 3-4 with conc. HCI (168 g) at 17-19 °C. The organic layer was separated and washed with water (2x1.5 L). The organic layer was concentrated in vacuo and dried by additional MTBE distillation. Toluene (2 x 500 mL) was added and distilled to remove moisture to provide 603 g of 2 as a light yellow-colored oil [Note: the water content by KF analysis was 508 ppm], Based on drying a small sample of 2 to a solid, the contained weight of 2 was 412 g (96% yield, not corrected for purity). ¹H NMR (300 MHz, CDCh): 07.34 (m, 3H), 7.29 (m, 2H), 5.24-5.11 (m, 2H), 4.52 (m, 1H), 4.43 (m, 1H), 3.64-3.42 (m, 2H), 2.27-2.09 (m, 2H). 0.85 (s, 9H), 0.06 (s. 3H), 0.04 (s, 3H) ppm; ¹³C NMR (75 MHz, d₆DMSO), mixture of rotomers: 0178.7, 178.4, 159.3, 158.9, 141.9, 141.8, 133.4, 133.3, 132.8,

132.6, 132.3, 131.9, 75.3, 74.6, 71.0, 71.0, 62.8, 62.3, 60.1, 59.7, 44.4, 43.4, 30.6, 30.6, 22.6,

22.6, 0.1, 0.0 ppm. Mass spectrum (ESI), m/z 379.5 [(M)+; calcd for Ci₉H₂8NO₅Si: 379.5].

Scheme II

3

4-(tert-Butvl-dimethvl-silanyloxv)-2-(6-fluoro-1H-indole-3-Oarbonvl)-pyrrolidine-1-carboxylic acid benzyl ester (3): Z-Hyp(OTBS)-OH (2, 55.5 g, 145 mmol) was dissolved in toluene (265 mL). DMF (0.1 mL) and oxalyl chloride (22.4 g, 174 mmol) were added at ambient température. After 2-3 h, the bubbling stopped. After 4 h, the mixture was concentrated in vacuo (65 °C bath, ca. 30 min) to provide 95 g of a light yellow-colored solution which was confirmed to be acid chloride by ¹H NMR analysis.

6-Fluoroindole (39.2 g, 290 mmol) was dissolved in anhydrous chlorobenzene (300 mL) and toluene (200 mL) and the solution was cooled to -4 °C using an ice/acetone bath. A solution of 3M EtMgBr in diethyl ether (101 g, 294 mmol) was added over 31 minutes at <2.5 °C resulting in a pale amber-colored solution. After 30 min, the acid chloride/toluene solution (vide supra) was added over 45 minutes at <2 °C. The reaction mixture was kept cold for 1 h then allowed to slowly warm. After ca. 4 h (10.6 °C), the reaction mixture was quenched with glacial HOAc (9.0 g, exothermic to 17.5 °C) and then water (exothermic). Water (200 mL) and EtOAc (300 mL) were added and the organic layer was separated and washed with water (100 mL, slow séparation). The organic layer was concentrated in vacuo to afford 227 g of 3 as an ambercolored oil which was used without further purification. ¹H NMR (300 MHz, CDCIg), ~2:1 mixture of rotomers: 09.38 (m, 0.7H), 8.58 (m, 0.3H), 8.35 (app. dd, J = 5.2, 8.2 Hz, 0.3H), 8.03 (app. dd, J = 5.2, 8.2 Hz, 0.7H), 7.74 (d, J = 2.9 Hz, 0.7H), 7.66 (d. J = 2.9 Hz, 0.3H), 7.38-7.32 (m, 5H), 7.07 (m, 1H), 6.95 (m, 1H), 6.85 (m, 1H), 5.26-4.92 (m, 3H), 4.54 (m, 1H), 3.80 (app. dt, J = 5.2, 11.1 Hz, 1H), 3.61 (app. d, J = 11.1 Hz, 0.3H), 3.55 (app. d, J= 11.1 Hz, 0.7H), 2.25-2.07 (m, 2H), 0.88 (s, 9H), 0.06 (s, 3H), 0.00 (s, 3H) ppm; ¹³C NMR (75 MHz, d_e-DMSO), mixture of rotomers: Π193.4, 193.0, 159.3 (d, J_CF = 235.5 Hz), 153.9 (d, J_CF = 16.2 Hz), 136.7, 136.8 (d, Jcf = 34.0 Hz), 134.6, 128.3, 127.8, 127.2, 126.6, 122.4, 113.7 (d, J_Cf= 13.5 Hz), 110.2 (d, J_CF = 20.2 Hz), 98.5 (d, J_CF - 25.4 Hz), 70.6, 69.8, 65.8, 65.8, 60.6, 60.3, 55.5, 55.0, 25.7, 25.6,

17.7, 17.7, -4.8, -4.9 ppm. Mass spectrum (ESI), m/z 518.9 [[(M-H)+Na]+; calcd for C₂₇H₃2FN2O₄SiNa: 518.6],

Scheme III

4

2-(6-Fluoro-1H-indole-3-carbonvl)-4-hydroxv-pyrrolidine-1-carboxylic acid benzyl ester (4): To a solution containing 3 (227 g) in THF (600 mL) was added 1 M TBAF in THF (160 mL) at ambient température. After 9 h, another 20 mL of the 1 M TBAF/THF solution was added. After ca. 48 h, the reaction mixture was concentrated in vacuo and then redissolved in EtOAc (600 mL). The organic solution was washed with water (310 mL) and the product precipitated to form a thick suspension which was filtered (slow). The solids were washed with EtOAc (165 mL in portions) and dried to provide 43 g of 4. The combined filtrate was concentrated in vacuo to precipitate an additional 4.8 g of 4 after drying. ¹H NMR (300 MHz, d6-DMSO), mixture of rotomers: 012.08 (br s, 1H), 8.43 (d, J = 10.5 Hz, 1H), 8.16 (ddd, J = 5.4, 8.7, 14.1 Hz, 1 H), 7.36-7.31 (m, 2H), 7.27 (app. d, J = 10.2 Hz, 1H), 7.09-6.93 (m, 4H), 5.24 (dt, J = 8.1, 15.6 Hz, 1H), 5.14 (br s, 1H), 5.04 (app. d, J = 6.4 Hz, 1H), 4.90 (app. dd, J= 13.4, 28.4 Hz, 1H), 4.30 (br s, 1H), 3.58-3.43 (m, 2H), 2.27 (m, 1H), 1.93 (m, 1H) ppm; ¹³C NMR (75 MHz, d0-DMSO), mixture of rotomers: Π194.0, 193.6, 159.9 (d, J_CF = 235.2 Hz), 154.6 (d, J_Cf = 9.6 Hz), 138.1. 137.5 (d, Jcf = 26.9 Hz), 136.0, 129.0, 128.5, 128.1 (d, J_CF = 40.0 Hz), 123.4, 123.3, 123.0,

122.9, 114.4 (d, J_CF = 11.7 Hz), 110.6 (d, J_CF = 23.7 Hz), 99.3 (d, J_CF = 25.2 Hz), 69.5, 68.8,

66.4, 66.3, 61.4, 61.1, 56.2, 55.7 ppm. Mass spectrum (ESI), m/z 382.6 [(M)+; calcd for C^H^FNzO^: 382.3].

Scheme IV

2-(6-Fluoro-1H-indole-3-carbonyl)-4-(4-nitro-benzoyloxy)-pyrrolidine-1-carboxvlic acid benzyl ester (5): A solution containing 4 (51.1 g, 134 mmol), 4-nitrobenzoic acid (27.9 g, 167 mmol) and triphenylphosphine (48.9 g, 187 mmol) in anhydrous THF (700 mL) and DMF (175 mL) was cooled to 2 °C. DIAD (37.4 mL, 194 mmol) was added over 1 h at 2-3 °C. After 1 h, the solution was allowed to warm to ambient température. After ca. 16 h, the reaction mixture was concentrated in vacuo and MeOH (250 mL) was added and concentrated to form a thick suspension (322 g). Additional MeOH (250 mL) was added and the solution was concentrated in vacuo to afford a thick suspension (420 g) that was chilled in an ice bath. After ca. 1.5 h, the solid was collected on a vacuum filter and washed with chilled MeOH (190 mL). The product was air-dried on the filter to provide 82.9 g (>100%) of 5 as a light yellow-colored solid which was used directly in the next reaction. ¹H NMR (300 MHz, de-DMSO), mixture of rotomers: □12.14 (br s, 1H), 8.47 (app. d, J = 6.6 Hz, 1H), 8.29-8.21 (m, 3H), 8.03 (dd, J = 2.7, 8.4 Hz, 2H), 7.43-7.33 (m, 2H), 7.28 (app. dd, J = 2.1, 9.6 Hz, 1 H), 7.20-7.08 (m, 4H), 5.55 (br s, 1H), 5.42 (dd, J= 8.4, 15.3 Hz, 1H), 5.13 (dd, J= 12.6, 22.2 Hz, 1H), 5.04 (s, 1H), 3.99 (m, 1H), 3.73 (d, J= 12.3 Hz, 1H), 2.91 (m, 1H), 2.36 (m, 1H) ppm; ¹³C NMR (75 MHz, de-DMSO), mixture of rotomers: 0192.9, 192.4, 164.2, 160.0 (d, J_Cf = 235.5 Hz), 154.5 (d, J_CF = 12.0 Hz), 150.9, 137.5, 137.1 (d, J_CF = 12.6 Hz), 135.6, 135.1, 131.3, 128.9 (d, J_CF = 28.0 Hz), 128.5, 128.2,

128.1, 127.6, 124.2, 123.0, 113.5 (d, J_CF = 8.5 Hz), 110.9 (d, J_Cf = 21.9 Hz), 99.1 (d, J_CF = 25.5 Hz), 75.2, 74.3, 66.7, 66.5, 62.4, 62.1, 53.6, 53.0, 38.6, 37.6 ppm. Mass spectrum (ESl), m/z 531.8 [(M)+; calcd for C2₈H₂2FN₃O₇: 531.5],

Scheme V

ΓΤ

2-(6-Fluoro-1H-indole-3-carbonvl)-4-hvdroxv-pyrrolidine-1 -carboxylic acid benzyl ester (6): To a suspension of 5 (82.9 g) in THF (600 mL), MeOH (200 mL), and water (100 mL) was added 50% aq. NaOH (16.0 g, 200 mmol) [Note: exothermic; temp. increase: 23.7 °C to 25.9 °C], After 2 h, glacial HOAc (5.3 g) was added to adjust the pH to 7-8 [Note: the orange-colored solution changed to pale yellow] and the réaction mixture was concentrated in vacuo. Water (500 mL) was added and solvent was removed in vacuo until a thick suspension formed. The solid was collected on a vacuum filter and washed with water (400 mL in portions). The solid was dried in a vacuum oven at 55 °C to afford 42.6 g (83%, 2 steps) of 6 as an off-white solid. ’H NMR (300 MHz, d₆-DMSO): 08.38 (d, J= 11.1 Hz, 1H), 8.14 (ddd, J = 5.7, 8.7, 14.1 Hz, 1H), 7.35-7.29 (m, 2H), 7.25 (app. dd, J = 2.1, 9.9 Hz, 1H), 7.10-6.95 (m, 4H), 5.16-4.98 (m, 2H), 4.90 (app. q, J =

13.5, 25.8 Hz, 1H), 4.26 (m, 1H), 3.74 (app. ddd, J= 6.3, 11.1, 18.3 Hz, 1H), 3.22 (m, 1H), 2.59 (m, 1H), 1.73 (app. ddd, 7 = 6.6, 12.9, 25.2 Hz, 1H) ppm;¹³C NMR (75 MHz, d₆-DMSO): Π193.8, 193.3, 160.0 (d, 7_Cf = 235.2 Hz), 154.4 (d, 7_Cf = 14.5 Hz), 137.5 (d, J_CF = 26.0 Hz), 137.2 (d, J_CF = 12.3 Hz), 129.0, 128.5, 128.2 (d, 7_CF = 35.4 Hz), 128.1, 127.4, 123.2, 123.1, 114.4 (d, 7_CF = 11.4 Hz), 110.8 (d, 7_Cf = 23.7 Hz), 110.8 (d, 7_CF = 23.7 Hz), 99.0 (d, J_CF = 25.8 Hz), 69.4, 68.6,

66.5, 66.4, 61.5, 61.2, 54.9, 54.6 ppm. Mass spectrum (ESI), m/z 383.8 [(M+H)+; calcd for C2iH₂₀FN₂O₄: 383.3],

Scheme VI

2-(6-Fluoro-1H-Îndol-3-vlmethvl)-4-hvdroxv-pyrrolidine-1-carboxylic acid benzyl ester (7): To a suspension of 6 (10.1 g, 26 mmol) in anhydrous THF (200 mL) was added 2M LiBH₄ in THF (26.2 mL, 52 mmol) over ca. 7 min [Note: exothermic; temp. increase: 21.5 °C to 28.2 ⁰C]. After 2.5 h, the pale, yellow-colored solution was cooled to ca. 11 °C and methanesulfonic acid (4.66 g, 48 mmol) was added over ca. 4 min [Note: exothermic; temp. increase to 14.2 °C],

After 16 h, the reaction mixture was cooled in an îce-bath and carefully quenched with water (50 mL) [Note: the addition of water was exothermic and released a large quantity of gas],

916572

Following the addition of water, the pH was adjusted to 1 with conc. HCl (1.9 g). The reaction mixture was concentrated to remove THF and the aqueous solution was extracted with EtOAc (110 mL). The organic layer was separated and washed with water (2 χ 50 mL) [Note: final pH about 5]. The organic solution was concentrated in vacuo and azeotropically dried using anhydrous EtOAc to provide 10.2 g of 7 as a white foam [Note: 87.7 A% by HPLC analysis]. ¹H NMR (300 MHz, cfe-DMSO), -1:1 mixture of rotomers: Π 10.91 (app. d, J= 5.4 Hz, 1H), 7.69 (dd, J = 6.0, 8.4 Hz, 0.5H), 7.48-7.30 (m, 4.5H), 7.13-7.07 (m, 3H), 6.85 (app. t, J = 8.4 Hz, 0.5H), 6.58 (app. t, J= 9.9 Hz, 0.5H), 5.19-5.10 (m, 3H), 4.25 (brs, 1H), 4.03-3.96 (m, 1H), 3.55 (dd, J = 5.1, 11.4 Hz, 1H), 3.29 (d, J= 11.4 Hz, 1H), 3.17-2.98 (m, 2H), 1.79 (m, 2H) ppm. ¹³C NMR (300 MHz, dc-DMSO), mixture of rotomers: 0159.5 (d, J_CF = 232.1 Hz), 159.4 (d, J_CF = 232.3 Hz), 154.9, 137.7 (d, J_CF = 36.6 Hz), 136.7 (d, J_Cf = 12.6 Hz), 136.6 (d, J_CF = 12.9 Hz), 129.1,

129.1, 128.7, 128.6 (d, J_CF = 26.3 Hz), 128.2, 125.0 (d, J_CF = 21.4 Hz), 124.5, 124.3, 120.1 (d, Jcf - 28.3 Hz), 120.0 (d, J_CF = 28.6 Hz), 112.4 (d, J_CF = 14.6 Hz), 107.4 (d, J_Cf = 24.3 Hz), 107.3 (d, J_CF = 24.3 Hz), 69.9, 69.2, 67.1, 66.3, 58.7, 58.1, 56.1, 55.6, 38.3, 37.6, 31.2, 30.1 ppm. Mass spectrum (ESl), m/z 368.6 [(M)+; calcd for C₂₁H₂₁FN₂O₃: 368.4],

Scheme VII
OH	F 9Ac	F
	(/¾ Αο,Ο. DMAP. DCM	O
cp°-A 7	8
4-Acetoxv-2-(6-fluoro-1 H-indol-3-vlmethvl)-pvrrolidine-1 -carboxylic acid	benzvl ester (8): To a

solution containing 7 (4.7 g, 12.8 mmol) and DMAP (81 mg, 0.66 mmol) in DCM (100 mL) was added acetic anhydride (2.6 g, 25.5 mmol) at ambient température. After 16 h, the reaction mixture was quenched with a MeOH (ca. 3 mL) and washed successively with 10% aq. Na₂CO₃ (50 mL), dilute HCl (50 mL), and 10% aq. Na₂CO₃ (50 mL). The organic solution was concentrated in vacuo and filtered through a short column of silica gel (ca. 25 g) [eluant: DCM (200 mL) to 0.5% (v/v) MeOH/DCM (80 mL) to 2% MeOH/DCM (100 mL) to 5% MeOH/DCM (100 mL)]. The product-containing fractions were combined and concentrated to provide 3.28 g (63%) of 8 as a white foam [Note: 94.3 A% by HPLC analysis], ¹H NMR (300 MHz, CDCI3), -1:1 mixture of rotomers: 07.99 (m, 1H), 7.75-6.61 (m, 9H), 5.28 (m, 1H), 5.20 (m, 2H), 4.23 (m, 1H), 3.82 (dt, J = 5.4, 13.5 Hz, 1H), 3.60 (app. t, J = 13.2 Hz, 1H), 3.50 (d, J = 11.7 Hz, 0.5H), 3.31 (d, J = 12.9 Hz, 0.5H), 2.87 (dt, J = 5.1, 13.5 Hz, 1 H), 2.13 (s, 3H), 2.01 (m, 2H) ppm; ¹³C NMR (75 MHz, CDCI3), -1:1 mixture of rotomers: 0170.8, 160.2 (J_CF = 236.4 Hz),

155.2, 136.8, 136.6, 136.4, 128.9, 128.8, 128.5 (J_CF = 24.3 Hz). 124.5 (J_CF = 21.4 Hz), 123.0,

123.0, 120.0 (J_Cf = 27.1 Hz), 119.9 (J_CF = 26.0 Hz), 112.8 (J_CF = 10.5 Hz), 108.2 (J_CF = 24.3 Hz),

97.7 (J_CF = 25.7 Hz), 74.0, 73.2, 67.9, 67.2, 58.5, 57.6, 53.4, 53.0, 35.4, 34.6, 30.8, 29.7, 21.5 ppm. Mass spectrum (ESl), m/z 410.6 [(M)+; calcd for C23H23FN2O4: 410.4],

4-Acetoxv-2-r3^l-(4-acetoxv-1-benzyloxycarbonvl-pvrrolidin-2-vlmethvl)-6,6^,-difÎuoro-1H,rH[2,2'lbiindolvl-3-vlmethvll-pyrrolidine-1-carboxylic acid benzyl ester (9): A solution containing 8 (2.9 g, 7.1 mmol) in EtOAc (ca. 5 mL) was cooled in an ice-bath and pre-cooled TFA (20.3 mL) was added in one portion. The resulting yellow-colored solution was stirred at 2-4 °C. After 4.75 h, the cold reaction mixture was transferred (via canula) with stirring into a pre-cooled mixture of EtOAc (30 mL), and 25% aq. K₂CO₃ (80.7 g). The aqueous layer was separated and extracted with EtOAc (3 x 30 mL) and the combined organic extracts were washed with 10% aq. Na₂CO₃ (30 g). The organic solution was concentrated in vacuo and azeotropically dried using anhydrous EtOAc to afford 2.95 g of indolylindoline diastereomers as a yellow-colored foam which was used directly in the next reaction. Mass spectrum (ESl), m/z 821.3 [(M)+; calcd for C4₆H4₆F₂N4O_e: 820.9],

To a solution containing the indolylindoline diastereomers (2.95 g) in EtOAc (30 mL) was added DDQ (885 mg, 3.9 mmol) in one portion [Note: exothermic; temp. increase: 26 °C to 31.6 °C]. After 3 h, the dark orange/brown-colored réaction mixture was filtered through Celite® which was subsequently rinsed with EtOAc (50 mL). [Note: a second reaction performed at 0.5 mmol-scale was combined for work-up], The filtrate was washed with 10% aq. Na₂CO₃ (2 washes: 74 g, then 58 g). The organic layer was concentrated in vacuo to provide 2.14 g of 9 as a light brown-colored solid.

The Celite® pad was further rinsed with THF (100 mL) which was concentrated in vacuo to provide another 1.12 g of 9 as a beige-colored solid. The combined solids were dissolved in isopropyl acetate (iPrAc, 50 mL). The ÎPrAc solution was reduced to ca. 20 mL and resulting suspension was warmed to reflux, cooled to ambient température, and then placed in an icebath. After 1 h, the solid was collected by vacuum filtration, washed with ÎPrAc (10 mL) and dried in a vacuum oven to afford 2.13 g (65%, 2 steps) of 9 as a beige-colored solid [Note: -100 A% by HPLC analysis]. Ή NMR (300 MHz, CDCI₃): 1311.29 (brs, 2H), 7.57-7.36 (m, 14H), 6.90 (app. dt, J = 2.1, 9.3 Hz, 2H), 5.39-5.30 (m, 6H), 4.28 (t, J = 9.0 Hz, 2H), 3.84-3.73 (m, 4H), 3.66

(d, J= 13.2 Hz, 2H), 3.40 (dd, J= 12.0, 14.4 Hz, 2H), 2.31 (s, 6H), 2.17 (m, 2H), 2.05 (m, 2H) ppm; ¹³C NMR (75 MHz, CDCI₃): 0170.7, 161.9, 158.8, 156.3, 137.5, 137.3, 136.5, 128.9, 128.6, 128.5, 125.9, 118.8, 118.6, 108.8, 108.5, 108.3, 98.7, 98.3, 74.4, 68.0, 60.1, 53.5, 34.5,

28.9, 21.7 ppm. Mass spectrum (ESI), m/z 818.2 [(M)+; calcd for C^^îFîN^: 818.8],

Acetic acid 5-[3'-(4-acetoxy-pvrrolidin-2-vlmethyl)-6,6'-difluoro-1 H, 1 'H-[2,2‘lbiindolyl-3-ylmethvllpyrrolidin-3-vl ester (10): A suspension containing 9 (35 g, 42.7 mmol) in 1:1 EtOAc/MeOH (400 mL) was distributed into two 500 mL Parr bottles (ca. 200 mL/each), and charged with 10% Pdon-C (wet, 5000 mg/each, Aldrich®). The reaction mixture was pressurized to 50 PSI H₂ and shaken for 3 h. The reaction mixture was filtered through a pad of Celite® and the solids were washed with EtOAc. The clarified filtrate was concentrated in vacuo to afford 24 g of 10 as an off-white solid which was used directly in the next reaction. ¹H NMR (300 MHz, CDCI3): 013.10 (br s, 2H) 7.45 (dd, J = 5.2, 8.9 Hz, 2H), 7.03 (dd, J = 2.3, 9.8 Hz, 2H), 6.85 (m, 2H), 5.35 (m, 2H), 3.71 (m, 2H), 3.18-3.35 (m, 4H), 2.90-3.14 (m, 4H), 2.56 (m, 2H), 2.00-2.10 (m, 2H), 2.04 (s, 6H), 1.80-1.92 (m, 2H) ppm; ¹³C NMR (75 MHz, CDCI3): Π171.3, 161.7, 158.6, 136.1, 135.9,

130.5, 130.4, 125.4, 119.1, 118.9, 109.6, 108.0, 107.6, 97.6, 97.5, 75.1, 57.7, 51.6, 38.7, 32.8,

21.6 ppm. Mass spectrum (ESI), m/z 550.9 [(M)+; calcd for C₃₀H₃₂F₂N4O4: 550.6].

Acetic acid 5-(3^,-[4-acetoxv-1-(2-terÎ-butoxvcarbonylamino-butvrvl)-pyrrolidin-2-vlmethvl|-6.6^ldifluoro-1H,TH-[2,2’]bÎindolyl-3-vlmethyl}-1-(2-tert-butoxvcarbonylamino-butvryl)-pyrrolidin-3-yl ester (11): To a solution containing Boc-Abu-OH (20.4 g, 100 mmol) and HATU (42.0 g, 110 mmol) in anhydrous NMP (150 mL) at 0 °C was added NMM (16 mL, 150 mmol) followed by a solution of 10 (24 g, 42 mmol) in NMP (100 mL). The reaction mixture was slowly warmed to ambient température. After 16 h, the reaction mixture was diluted with MTBE (1000 mL) and the heterogeneous mixture was washed with water (500 mL). The layers were separated and the organic phase formed a heterogeneous suspension. MTBE (1000 mL) and EtOAc (500 mL) were added and the now-homogeneous solution was washed successively with 1 N HCl (2 x 100 mL), saturated aqueous NaHCO₃ (2 x 100 mL), brine, dried over anhydrous Na₂SO₄₁ filtered, and concentrated. The residue was dissolved in 1:1 DCM/ MeOH (600 mL) and DCM (ca. 200 mL) was removed via distillation at 50 °C [Note: a small quantity of white precipitate was observed], MeOH (200 mL) was added and additional solvent was removed (ca. 200 mL) at 50 °C. The heterogeneous mixture was cooled at -5 °C. After 16 h, the solid was collected by vacuum filtration and washed with cold MeOH. The solid was dried under high vacuum to afford 32 g of 11 as an off-white solid. ¹H NMR (300 MHz, CDCI3), mixture of rotomers: ΠΠ11.22 (br s, 2H), 7.40 (dd, J = 5.1, 8.7 Hz, 2H), 7.31 (d, J = 9.3 Hz, 2H), 6.76 (dd, J = 8.40, 8.40, 2H) 6.26 (br s, 2H), 5.44 (m, 2H), 4.39 (dd, J = 7.5, 16.5 Hz, 2H), 4.24 (m, 2H), 4.15 (dd, J = 5.1, 12.9 Hz, 2H), 3.79 (d, J= 12.9 Hz, 2H), 3.10-3.30 (m, 4H), 2.32 (d. J= 14.7 Hz, 2H), 2.24 (s, 6H), 1.90 (m, 2H), 1.74 (m, 2H), 1.56 (s, 18H), 0.99 (t, J = 7.5 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI3): 13172.2, 170.4, 161.4, 158.3, 155.8, 137.0, 136.9, 128.6, 125.5, 118.9, 118.7,

108.6, 108.4, 108.1, 98.3, 98.0, 80.8, 74.7, 60.4, 53.8, 53.5, 34.1, 28.7, 28.6, 26.2, 21.5, 10.5 ppm. Mass spectrum (ESI), m/z 920.5 [(M)+; calcd for C₄₀H₀2F₂N_eOi_O: 921.0].

Acetic acid 5-(3^,-[4-acetoxv-1-(2-amino-butvryl)-pvrrolidin-2-vlmethvn-6,6'-difluoro-1H.1^lH[2,2^,]biindolvl-3-vlmethvl)-1-(2-amino-butvrvl)-pyrrolidin-3-vl ester (12): A solution containing 11 (27.5 g, 30 mmol) in DCM (200 mL) was cooled to 0 °C. TFA (50 mL) was added and the reaction was monitored by LC/MS analysis until complété conversion of 11 to 12 (ca. 3 h). The solvent was removed in vacuo and the dark, green-colored residue was dissolved in EtOAc (ca. 1 L). The EtOAc solution was carefully poured into a saturated aqueous NaHCO₃/ice/water mixture to neutralize the residual TFA. The organic phase was separated and washed twice with saturated aqueous NaHCO₃ then once with brine. The combined aqueous washes were backextracted with EtOAc (2 χ 100 mL) and the combined organic extracts were dried over

anhydrous Na₂SO_4i filtered, and concentrated to afford 22 g of crude 12 as an off-white solid. ’H NMR (300 MHz, CDCI₃ + d₄-MeOH), mixture of rotomers: 011.62 (br s, 2H), 7.48-7.62 (m, 4H), 6.89 (ddd, J = 2.4, 9.3, 9.3 Hz, 2H), 5.48 (dd, J = 4.5, 4.8 Hz, 2H), 4.52 (dd, J = 9.3, 9.3 Hz, 2H), 4.06 (dd, J - 4.8, 12.3 Hz, 2H), 3.78 (d, J = 12.3 Hz, 2H), 3.54-3.70 (m, 4H), 3.30-3.40 (m, 2H), 2.33 (s, 6H), 2.02-2.16 (m, 2H), 1.70-1.96 (m, 4H), 1.09 (t, J = 7.2 Hz, 6H) ppm, ¹³C NMR (75 MHz, CDCh + d₄-MeOH): 0173.5, 170.9, 161.8, 158.6, 137.2, 137.1, 128.2, 128.1, 125.6,

118.7, 118.6, 108.6, 108.3, 108.0, 98.6, 98.1, 74.6, 60.1, 53.5, 33.5, 28.0, 21.4, 9.7 ppm. Mass spectrum (ESI), m/z 721.4 [(M)+; calcd for C_3BH₄₆F₂N₆O_e: 720.8].

Scheme XII

OAc OAc

Acetic acid 5-(3'-(4-acetoxy-1-f2-(2-methvl-(tert-butoxycarbonyl)-amino-propionvlamino)butvrvII-pvrrolidin-Z-vImethvn-e.e'-difluoro-IH.TH-fZ.Z'lbiindolvl-S-vImethvh-l-fZ-fZ-methvl-ftertbutoxvcarbonvl)-amino-propionvlamino)-butvrvll-pyrrolidin-3-vl ester (13): To a solution containing Boc-N(Me)Ala-OH (14.6 g, 72 mmol) and HATU (30.4 g, 80 mmol) in anhydrous NMP (150 mL) at 0 °C was added NMM (12 mL, 105 mmol) followed by addition of 12 (30 mmol) in NMP (200 mL). The resulting mixture was allowed to warm to ambient température. After 16 h, the reaction mixture was diluted with diethyl ether (1 L) and washed successively with water (1 L), 1N HCl (2 x 100 mL), saturated aqueous NaHCO₃ (2 x 100 mL), brine, dried over anhydrous Na₂SO₄, filtered, concentrated to afford 33.5 g of crude 13.

The crude 13 was dissolved in EtOH (50 mL) and then slowly added to water (1000 mL) with vigorous stirring at 50 °C which resulted in the précipitation of a white solid. The heterogeneous mixture was cooled to -5 °C. After 16 h, the solid was collected by vacuum filtration and washed with water. The wet solid was dried under high vacuum at 50 °C to afford

29.9 g of 13 as an off-white solid. ’H NMR (300 MHz, CDCI₃): 011.57 (brs, 2H), 7.40-7.60 (m, 4H), 6.89 (m, 2H), 5.50 (m, 2H), 4.75 (m, 2H), 4.67 (q, J = 6.9 Hz, 2H), 4.50 (t, J = 9.6 Hz, 2H), 4.20 (dd, J = 3.9, 12.3 Hz, 2H) 3.85 (d, J = 12.3 Hz, 2H), 3.57 (br d, J = 13.5 Hz, 2H), 3.34 (dd, J - 12.0, 13.8 Hz, 2H), 2.89 (s, 6H), 2.34 (s, 6H), 2.10 (m, 2H), 1.95 (dt, J = 6.0, 13.8 Hz, 2H), 1.79 (dt, J = 7.2, 14.1 Hz, 2H), 1.52 (s, 18H), 1.39 (d, J = 7.2 Hz, 6H), 1.03 (t, J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI₃): 0174.0, 172.1, 171.9, 170.5, 161.8, 158.7, 137.5, 137.3, 128.4, 125.8, 118.7, 118.6, 108.8, 108.4, 108.1, 98.8, 98.5, 81.0, 74.6, 60.1, 54.0, 52.0, 33.7, 30.5, 28.6, 28.1, 25.9, 21.6, 14.0, 9.9 ppm. Mass spectrum (ESI), m/z 1091.7 [(M)+; calcd for θίβΗζθΕΐΝβθιο· 1091.2],

Scheme XIII

Acetic acid 5-(3^l-f4-acetoxv-1-r2-(2-methvlamino-propionvlamino)-butvrvll-pvrrolidin-2-vlmethyl}6,6'-difluoro-1 H, 1 'H-[2,2'1biindolvl-3-vlmethyl)-1 -[2-(2-methylamino-propionvlamino)-butyrvl1pyrrolidin-3-yl ester (14): A solution containing 13 (28.5 g, 26 mmol) in DCM (150 mL) was cooled to 0 °C. TFA (50 mL) was added. After 30 min, the reaction mixture was warmed to ambient température and monïtored until LC/MS analysis revealed complété conversion of 13 to 14 (ca. 4 h). The solvent was removed in vacuo and the dark, green-colored residue was dissolved in EtOAc (500 mL) and carefully poured onto an aqueous NaHCO3/ice mixture. The aqueous phase was separated and back-extracted with EtOAc (2 x 250 mL). The combined organic extracts were washed several times with saturated aqueous NaHCO3, then brine, dried over anhydrous NazSO4, filtered, and concentrated to afford 24 g of 14 as a light yellow-colored solid. ¹H NMR (300 MHz, CDCl3): Π11.66 (brs, 2H), 8.16 (d, 8.4 Hz, 2H), 7.52 (dd, J= 2.1,

9.6 Hz, 2H), 7.43 (dd, J= 5.4, 8.4 Hz, 2H), 6.83 (ddd, J = 2.1,9.0, 9.0 Hz, 2H), 5.41 (dd, J= 4.2,

4.5 Hz, 2H), 4.64 (dd, J = 7.8, 14.1 Hz, 2H), 4.36 (br d, J = 9.3, 9.6 Hz, 2H), 4.13 (dd, J = 4.8,

12.6 Hz, 2H), 3.81 (d, J = 12.0 Hz, 2H), 3.44 (d, J = 13.2 Hz, 2H), 3.0-3.18 (m, 4H), 2.50 (s, 6H), 2.30 (s, 6H), 2.15 (d, J - 14.4 Hz, 2H), 1.90-2.08 (m, 2H), 1.76-1.90 (m, 2H), 1.33 (d, J = 7.2 Hz, 6H), 1.08 (t, J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCl₃): D175.3, 172.6, 170.4, 161.8, 137.5, 137.3, 128.4, 128.3, 125.9, 118.6, 118.5, 108.5, 108.1, 107.8, 98.7, 98.3, 74.5, 60.9,

59.9, 53.9, 51.3, 35.8, 33.6, 27.6, 26.2, 21.5, 20.2, 10.1 ppm. Mass spectrum (ESI), m/z 891.6 [(M)+; calcd for C_4eH_6OF_zNaO₀: 891.0].

Scheme XIV

N-{1S-f2R-(6,6^l-Difluoro-3^>-{4S-hvdroxv-142S-(2S-methvlamino-propionvlamino)-butyrvllpvrrolidin-2R-vlmethyl}-1H.TH-[2,2'1biindolv1-3-vlmethyl)-4S-hydroxv-pyrrolidine-1-carbonvllpropyl}-2S-methylamino-propionamide (15): To a solution containing 14 (24 g) in MeOH (200 mL) was added 1 M NaOH (80 mL) at 0 °C. The reaction mixture was degassed and maintained under a nitrogen atmosphère wrapped with aluminum foil. The ice-bath was removed. After 60 min, the MeOH was removed in vacuo and the residue was diluted with water (200 mL) and extracted with EtOAc (500 mL). The aqueous phase was separated and back-extracted with EtOAc (2 x 150 mL). The combined organic extracts were washed with brine and dried over anhydrous Na₂SO₄, filtered, and concentrated to afford 22.5 g of crude 15 as a light, brown/yellow-colored solid.

The crude 15 (22.5 g) was dissolved in MeOH (50 mL) and EtOAc (200 mL), The volume was reduced (50%) by distillation at reduced pressure at 60 °C using a rotary evaporator. MTBE (300 mL) was added and the cloudy solution was warmed to 60 °C. After 30 min, the solution was cooled to ambient température and then maintained at -5 °C.

After 16 h, the solid was collected by vacuum filtration and washed with cold 25% EtOAc/MTBE and dried under high vacuum at ambient température to afford 16.6 g of 15 as an off-white solid. An additional 5.5 g of 15 was recovered from the filtrate via solvent removal and vacuum drying. ¹H NMR (300 MHz, CDCI₃): Π11.74 (s, 2H), 8.27 (d, J = 8.7 Hz, 2H), 7.71 (dd, J = 5,4, 8.4 Hz, 2H), 7.55 (dd, J =2.4, 9.6 Hz, 2H), 6.88 (ddd, J = 2.4, 9.3, 9.3 Hz, 2H), 4.62-4.78 (_m, 4H), 4.43 (dd, J = 9.3, 9.9 Hz, 2H), 4.03 (dd, J = 4.8, 11.4 Hz, 2H), 3.80 (d, J = 11.4 Hz, 2H), 3.66 (dd, J = 2.7, 14.4 Hz, 2H), 3.53 (dd, J = 11.4, 14.4 Hz, 2H), 3.11 (q, J = 6.9 Hz, 2H), 2.56 (s, 6H), 2.45 (m, 2H), 2.19 (d, J = 14.4 Hz, 2H), 1.76-2.10 (m, 6H), 1.59 (br s, 2H), 1.39 (d, J =

6,9 Hz, 6H), 1.22-1.38 (m, 2H), 1.07 (t. J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, cfe-DMSO): □ 175.2, 172.8, 161.6, 158.5, 137.3, 137.2, 128.4, 128.3, 126.4, 120.8, 120.6, 109.4, 108.7, 108.4, 98.4, 98.0, 70.8, 60.2, 59.9, 56.6, 51.8, 36.4, 35.3, 28.3, 25.6, 20.0, 10.6 ppm. Mass spectrum (ESI), m/z 807.5 [(M)+; calcd for 042Η₅θΡ2Ν₈Ο_θ: 806.9],

Scheme XV

/V-tert-butoxycarbonvl-A/-(d₃-methyl)alanine (17): To a solution of Boc-Ala-OH (16, 3.5 g, 18.5 mmol) in anhydrous THF (50 mL) was added NaH (2.1 g, 60% in minerai oil, 51.0 mmol) at 0 °C. After 45 min, the reaction mixture was warmed to ambient température and then warmed to 45 °C for an additional 20 min. The reaction mixture was cooled to 0 °C and d₃-iodomethane (10.0 g, 69.0 mmol) was added. The resulting mixture was stirred at ambient température. After 16 h, the reaction mixture was quenched with water, and extracted with EtOAc. The organic phase was discarded and the aqueous solution was acidified to pH 3 with 1N HCl and extracted with EtOAc. The organic phase was washed with brine, dried over anhydrous Na₂SO4, filtered, and concentrated. The residue was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min) to afford 17 (3.6 g, 94%) as a white solid following lyophilization. . ¹H NMR (300 MHz, cL-MeOH), mixture of rotomers;

□4.80 (br s, 1 H), 4.67 (q, J = 6.9 Hz, 0.5H), 4.38 (q, J = 6.9 Hz, 0.5H), 1.36-1.52 (m, 12H) ppm; Mass spectrum (ESl), m/z 207.0 [(M+H)+; calcd for C9H15D3NO4: 207.2].

Scheme XVI

butyrvll-pvrrolidin-2-vlmethvl}-6_l6^,-difluoro-1H,TH-f2.2^,lbiindolvl-3-ylmethvl)-1-[2-(2-drmethvl(tert-butoxycarbonyl)-amino-propionvlamino)-butyryll-pvrrolidin-3-yl ester (18): To a solution containing Boc-N(d3-Me)Ala-OH (17, 1.00 g, 4.83 mmol) and HATU (2.00 g, 5.30 mmol) in anhydrous NMP (20 mL) at 0 °C was added NMM (0.8 mL, 7.20 mmol) followed by addition of 12 (crude, 1.73 g, 2.40 mmol) in NMP (20 mL). The resulting mixture was allowed to warm to ambient température. After 16 h, the réaction mixture was diluted with diethyl ether (200 mL) and washed successively with water (200 mL), 1N HCl (2 x 100 mL), saturated aqueous NaHCOg (2 x 100 mL), brine, dried over anhydrous Na2SO4, filtered, concentrated. The residue was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, frozen, and lyophilized to afford 1.1 g of 18 (42%) as an off-white solid. ¹H NMR (300 MHz, CDCI3), mixture of rotomers: Π11.56 (br s, 2H), 7.56 (dd, J = 5.4, 8.7 Hz, 2H), 7.52 (m, 2H), 7.10 (br s, 2H), 6.89 (ddd, J = 2.1, 9.0, 9.0 Hz, 2H), 5.47 (t, J = 4.8 Hz, 2H), 4.75 (br s, 2H), 4.67 (q, J= 6.9 Hz, 2H), 4.50 (t, J = 9.3 Hz, 2H), 4.18 (dd, J = 4.2, 11.7 Hz, 2H) 3.85 (d, J= 12.6 Hz, 2H), 3.57 (dd, J = 2.1, 14.4 Hz, 2H), 3.34 (dd, J ~ 12.0, 14.4 Hz. 2H), 2.34 (s, 6H), 2.29 (br s, 2H), 2.10 (m, 2H), 1.97 (m, 2H), 1.79 (m, 2H), 1.51 (s, 18H), 1.39 (d, J = 6.9 Hz, 6H), 1.03 (t, J = 7.5 Hz, 6H) ppm. Mass spectrum (ESl), m/z 1097.7 [(M)+; calcd for C₅₆H7oD₆F2N80i₂: 1097.3].

Scheme XVII

Acetic acid 5-(3’-(4-acetoxv-1-f2-(2-d-methvlamino-propionvlamino)-butvrvl1-pyrrolidin-2vlmethviP6,6^,-difluoro-1H.TH-r2,2']biindolvl-3-ylmethvl)-142-(2-d_rmethvlamino-propionvlamino)butyrvll-pyrrolidin-3-yl ester (19): A solution containing 18 (1.10 g, 1.00 mmol) in DCM (15 mL) was cooled to 0 °C. TFA (5 mL) was added. After 30 min, the reaction mixture was warmed to

ambient température and monitored until LC/MS analysis revealed complété conversion of 18 to 19 (ca. 4 h). The solvent was removed in vacuo and the dark, green-colored residue was dissolved in EtOAc (100 mL) and carefully poured onto an aqueous NaHCO₃/ice mixture. The aqueous phase was separated and back-extracted with EtOAc (2 χ 50 mL). The combined organic extracts were washed several times with saturated aqueous NaHCO₃, and then brine, dried over anhydrous Na₂SO«, filtered, and concentrated to afford crude 19 which was used without further purification. Mass spectrum (ESl), m/z 897.5 [(M)+; calcd for C46H₅4D₆F₂NbO_b: 897.0],

Scheme XVIII

N-{1S-[2R-(6.6^l-Difluoro-3^l-{4S-hvdroxv-1-f2S-(2S-d2-methvlamino-propionylamino)-butvrvl1pyrrolidin-2R-ylmethyl)-1 H, rH-[2.2'lbiindolvl-3-v1methyl)-4S-hvdroxv-pyrrolidine-1 -carbonyllpropvl}-2S-drmethvlamino-propionamide (20): To a solution containing crude 19 (ca. 1.00 mmol) in MeOH (20 mL) was added 1 M NaOH (2 mL) at ambient température. After 35 min, the MeOH was removed in vacuo and the residue was diluted with water (50 mL) and extracted with EtOAc (2 χ 50 mL). The combined organic extracts were washed with brine and dried over anhydrous Na2SO4, filtered, and concentrated. The residue was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, frozen, and lyophilized to afford 0.6 g of 20 (75%) as a flocculent, white solid. ¹H NMR (300 MHz, CD3CN), mixture of rotomers: □ 11.86 (s, 2H), 7.91 (d, J = 7.8 Hz, 2H), 7.71 (dd, J = 5.4, 8.7 Hz, 2H), 7.45 (dd, J = 2.4, 9.9 Hz, 2H), 6.83 (m, 2H), 4.56 (m, 2H), 4.47 (m, 2H), 4.20 (m, 2H), 3.84 (dd, J = 4.2, 11.1 Hz, 2H), 3.66 (d, J = 11.1 Hz, 2H), 3.45 (m, 4H), 2.93 (q, J = 6.9 Hz, 2H), 1.60-1.89 (m, 8H), 1.19 (d,J =

6.9 Hz, 6H), 0.94 (t, J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CD₃CN + d_rMeOH), mixture of rotomers: 0175.2, 173.0, 162.4, 159.3, 137.8, 137.6, 128.8, 128.7, 126.8, 110.8, 120.7, 109.5,

108.7, 108.4, 98.5, 98.1, 71.6, 60.5, 60.1, 56.8, 52.6, 36.6, 28.6, 26.0, 22.7, 19.0, 10.1 ppm. Mass spectrum (ESl), m/z 813.4 [(M)+; calcd for C4₂H5oDbF₂Nb0₆: 813.0],

Scheme XIX

.F

2-(6-Fluoro-1H-indol-3-yl-d?-methvl)-4-hvdroxv-pyrrolidine-1-carboxvlic acid benzyl ester (21): A suspension of 6 (3.0 g, 7.85 mmol) in anhydrous THF (50 mL) was cooled to 0 °C. d₄-NaBH₄ (0.66 g, 15.7 mmol) was added in one portion followed by the addition of BF₃ etherate (1.1 mL, 8.60 mmol). After ca. 10 min, the ice bath was removed and the reaction mixture was warmed to reflux.

After 3 h, the reaction mixture was cooled in an ice-bath and carefully quenched with saturated aqueous NH₄CI (50 mL). The biphasic mixture was diluted with EtOAc and the organic layer was separated and washed with water (2 x 50 mL) then brine. The EtOAc layer was dried over anhydrous Na₂SO₄, filtered, and concentrated to afford 3.2 g of crude 21 (>quant.) which was used without further purification. Mass spectrum (ESI), m/z 371.2 [(M+H)+; calcd for C₂iH₂oD₂FN₂0₃: 371.4],

Scheme XX

4-Acetoxy-2-(6-fluoro-1H-indol-3-vl-d₂-methvl)-pyrrolidine-1-carboxvlic acid benzyl ester (22): To a solution containing crude 21 (ca. 7.85 mmol), Et₃N (1.2 g, 12.0 mmol), and DMAP (50 mg, cat.) in DCM (30 mL) was added acetic anhydride (0.74 mL, 7.85 mmol) at ambient température. After 3 h, the reaction mixture was quenched with saturated aqueous NaHCO₃ (50 mL) then diluted with DCM. The DCM layer was separated and washed successively with dilute HCl (50 mL), water (50 mL), and brine (50 mL). The organic solution was dried over anhydrous Na₂SO₄, filtered, and concentrated. The crude product was purified by flash silica gel chromatography [30-40% EtOAc in hexane] to afford 2.0 g (62%, 2 steps) of 22 as a white foam. ¹H NMR (300 MHz, CDCI3), -1:1 mixture of rotomers: D8.41 (br s, 1H), 7.80-6.50 (m, 9H), 5.25 (m, 1H), 5.21 (m, 2H), 4.27 (m, 1H), 3.82 (dt, J = 5.1, 13.2 Hz, 1H), 3.61 (dd, J= 11.4, 11.7 Hz, 1H), 2.13 (s, 3H), 2.00 (m, 2H) ppm; ¹³C NMR (75 MHz, CDCI3), -1:1 mixture of rotomers: □ 170.8, 160.2 (Jcf = 236.2 Hz), 155.2, 136.9, 136.6, 136.5, 129.0, 128.9, 128.6 (J_CF = 24.4 Hz),

124.5 (J_Cf = 22.1 Hz), 123.1, 120.1 (J_CF = 27.2 Hz), 119.9 (J_Cf = 27.2 Hz), 112.8, 108.2 (J_Cf -

23.5 Hz), 97.7 (J_Cf = 25.7 Hz), 74.1, 73.3, 68.0, 67.2, 58.5, 57.6, 53.4, 53.1, 35.4, 34.6, 21.5 ppm. Mass spectrum (ESI), m/z 413.1 [(M)+; calcd for C₂3H₂₁D2FN₂O₄: 412.4].

Scheme XXI

4-Acetoxv-2-r3'-(4-acetoxv-1-benzvloxvcarbonvl-pvrrolidin-2-yl-d?-methvl)-6,6^l-difluoro-1H,1^lH[2,2^l1biindolvl-3-vl-dg-methvll-pyrrolidine-1-carboxylic acid benzyl ester (23): Indole 22 (2.0 g, 4.80 mmol) was dissolved in pre-cooled (-5 °C) TFA (10 mL). The resulting yellow-colored solution was allowed to slowly warm to ambient température over 2 h. The reaction mixture was concentrated in vacuo to remove the TFA and the crude mixture of îndolylindoline diastereomers was used directly in the next reaction. Mass spectrum (ESI), m/z 825.4 [(M)+; calcd for C_4eH₄2D₄F₂N₄O₀: 824.9],

To a solution containing the îndolylindoline diastereomers in EtOAc (100 mL) was added DDQ (0.58 g, 2.5 mmol) in one portion. After 15 min, the dark orange/brown-colored reaction mixture was quenched with saturated aqueous NaHCO₃. The layers were separated and the organic phase was washed successively with saturated aqueous NaHCO₃ (3 x 50 mL) and brine, dried over anhydrous Na₂SO₄, filtered, and concentrated. The crude product was dissolved in DCM (10 mL) and the solution was then diluted with MeOH (50 mL). Slow removal of the DCM in vacuo afforded a precipitate which was collected by vacuum filtration, washed with cold MeOH, and dried to provide 1.7 g of 23 (86%, 2 steps). ¹H NMR (300 MHz, CDCI₃): □11.30 (br s, 2H), 7.60-7.30 (m, 14H), 6.90 (app. dt, J = 2.4, 9.3 Hz, 2H), 5.40 (m, 2H), 5.36 (d, J = 3.6 Hz, 4H), 4.28 (d, J = 8.1 Hz, 2H), 3.79 (m, 4H), 2.31 (s, 6H), 2.06 (m, 4H) ppm; Mass spectrum (ESI), m/z 823.3 [(M)+; calcd for C₄₈H4oD₄F₂N₄0₈: 822.9].

Acetic acid 5-r3'-(4-acetoxv-pvrrolidin-2-vl-d_?-methvl)-6_l6^l-difluoro-1 H, 1 ^,H-f2,2¹lbiindolvl3-yl-dg-methyl]-pyrrolidin-3-yl ester (24): A suspension containing 23 (0.40 g, 0.48 mmol) in 1:1 EtOAc/MeOH (40 mL) was placed in a 500 mL Parr bottle and charged with 10% Pd-on-C (wet, ca. 200 mg). The réaction mixture was pressurized to 50 PSI H₂ and shaken for 3 h. The réaction mixture was filtered through a pad of Celite® and the solids were washed with EtOAc. The clarified filtrate was concentrated in vacuo to afford crude 24 as an off-white solid which was used directly in the next reaction. Mass spectrum (ESl), m/z 555.2 [(M)+; calcd for C30H2BD4F2N4O4: 554.6],

Acetic acid 5-{3'-i4-acetoxy-1 -(2-tert-butoxycarbonylarriino-butvrvl)-pvrrolidin-2-vl-d_?-methyl]e.e'-difluoro-l H, TH-[2,2'1biindolvl-3-vl-dg-methylM -(2-tert-butoxvcarbonvlamino-butyrvl)pyrrolidin-3-yl ester (25): To a solution containing Boc-Abu-OH (224 mg, 1.1 mmol) and HATU (442 mg, 1.2 mmol) in anhydrous NMP (10 mL) at 0 °C was added NMM (0.2 mL, 1.7 mmol) followed by a solution of 24 (0.48 mmol) in NMP (10 mL). The reaction mixture was slowly warmed to ambient température. After 16 h, the reaction mixture was diluted with diethyl ether (100 mL) and the mixture was washed successiveiy with water (5 x 50 mL), 1N HCl (50 mL), saturated aqueous NaHCO₃ (50 mL), and brine, dried over anhydrous Na₂SO4, filtered, and concentrated. The crude product was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The productcontaining fractions were combined, concentrated, and lyophilized to afford 310 mg of 25 (70%, 2 steps) as a flocculent white solid. ¹H NMR (300 MHz, CDCI3), mixture of rotomers: Π11.17 (br s, 2H), 7.39 (dd, J = 5.4, 8.4 Hz, 2H), 7.29 (d, J = 9.3 Hz, 2H), 6.75 (dd, J - 8.40, 8.40, 2H), 6.40 (br s, 2H), 5.44 (m, 2H), 4.40 (dd. J = 7.8, 16.5 Hz, 2H), 4.22 (d, J = 7.8 Hz, 2H), 4.15 (dd, J - 5.1, 12.9 Hz, 2H), 3.80 (d, J= 12.9 Hz, 2H), 2.23 (s, 6H), 1.90 (m, 2H), 1.74 (m, 2H), 1.57 (s, 18H), 0.99 (t, J= 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI3), mixture of rotomers: Π172.1, 170.4, 161.4, 158.2, 155.8, 137.0, 136.9, 128.6, 125.5, 118.9, 118.8, 108.6, 108.4, 108.1, 98.3, 98.0, 80.8, 74.7, 60.3, 53.8, 53.6, 34.1, 28.7, 28.6 (br), 26.2, 21.5, 10.5 ppm. Mass spectrum (ESl), m/z 925.4 [(M)+; calcd for CwHseD^FzNeOw: 925.0],

Scheme XXIV

Acetic acid 5-{3’-[4-acetoxv-1 -(2-amino-butvrvl)-pvrro1idin-2-vl-d₂-methvll-6,6'-difluoro-1 H, 1ΉÎZ.Z’lbiindolvl-S-vl-dg-methvO-l-fë-amino-butvrvb-pyrrolidin-S-yl ester (26): A solution containing 25 (310 mg, 0.34 mmol) in DCM (20 mL) was cooled to 0 °C. TFA (5 mL) was added and the reaction was monitored by LC/MS analysis until complété conversion of 25 to 26 (ca. 3 h). The solvent was removed in vacuo and the dark, green-colored residue was dissolved in EtOAc (50 mL). The EtOAc solution was carefully poured into a saturated aqueous NaHCO₃/ice/water mixture to neutralize the residual TFA. The organic phase was separated and washed twice with saturated aqueous NaHCO₃ then once with brine. The combined aqueous washes were backextracted with EtOAc (2 x 20 mL) and the combined organic extracts were dried over anhydrous Na₂SO_4i filtered, and concentrated to afford crude 26 (250 mg) as an off-white solid. Mass spectrum (ESl), m/z 725.3 [(M)+; calcd for C_3BH«D4F₂N_SO₆: 724.8].

Acetic acid 5-(3'-f4-acetoxy-1 -f2-(2-methvl-(tert-butoxvcarbonyl)-amino-propionvlamino)butvrvII-pvrrolidin-Z-vl-dp-methvll-e.S’-difluoro-IH.TH-fë^lbÎindolvl-S-vl-dirmethvO-W-fZmethvl-(tert-butoxvcarbonvl)-amino-propionylamino)-butvrvl1-pyrrolidin-3-vl ester (27): To a solution containing Boc-N(Me)Ala-OH (83 mg, 0.41 mmol) and HATU (172 mg, 0.45 mmol) in anhydrous NMP (5 mL) at 0 °C was added NMM (0.1 mL, 0.85 mmol) followed by addition of crude 26 (123 mg, 0.17 mmol) in NMP (5 mL). The resulting mixture was allowed to warm to ambient température. After 16 h, the reaction mixture was diluted with diethyl ether (100 mL) and washed successively with water (50 mL), 1N HCl (2 x 50 mL), saturated aqueous NaHCO₃ (2 x 50 mL), brine, dried over anhydrous Na₂SO₄₁ filtered, concentrated. The crude product was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing

0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, concentrated, and lyophilized to afford 170 mg of 27 (91%, 2 steps) as a flocculent, off-white solid. ¹H NMR (300 MHz, CDCI3), mixture of rotomers: 011.51 (br s, 2H), 7.40-7,60 (m, 4H), 6.86 (m, 2H), 5.46 (m, 2H), 4.74 (br s, 2H), 4.65 (q, J = 6.9 Hz, 2H), 4.45 (d, J = 8.7 Hz, 2H), 4.17 (dd, J = 4.8, 12.3 Hz, 2H) 3.82 (d, J - 12.3 Hz, 2H), 2.87 (s, 6H), 2.28 (s, 6H), 2.05 (m. 2H), 1.92 (m, 2H), 1.78 (m, 2H), 1.48 (s, 18H), 1.37 (d. J = 7.2 Hz, 6H), 1.01 (t, J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI3), mixture of rotomers: Π173.3, 170.2, 170.1, 170.5, 168.6, 159.9, 135.5, 135.4, 126.5, 126.4, 123.8, 116.8, 116.7, 106.8, 106.4, 106.1, 96.8, 96.5, 79.1, 72.6, 57.9, 52.1, 50.1, 31.7, 28.5, 26.6, 25.5 (br), 23.9, 19.6, 19.0, 12.1, 8.0 ppm. Mass spectrum (ESI), m/z 1095.5 [(M)+; calcd for C56H72D4F2N6OJ2: 1095.3].

Scheme XXVI

Acetic acid 5-(3'-f4-acetoxy-1 -r2-(2-methvlamino-propionvlaminoÎ-butvryl1-pvrrolidin-2-vl-d_r rnethy))-6,6‘-difluoro-1 H, 1 'H-[2,21biindolyl-3-vl-d_rmethyl)-1 -f2-(2-methvlamino-propionylarnino)butvryll-pyrrolidin-3-yl ester (28): A solution containing 27 (170 mg, 0.15 mmol) in DCM (15 mL) was cooled to 0 °C. TFA (5 mL) was added. After 30 min, the reaction mixture was warmed to ambient température and monitored until LC/MS analysis revealed complété conversion of 27 to 28 (ca. 4 h). The solvent was removed in vacuo and the dark, green-colored residue was dissolved in EtOAc (100 mL) and carefully poured onto an aqueous NaHCO₃/ice mixture. The aqueous phase was separated and back-extracted with EtOAc (2 x 20 mL). The combined organic extracts were washed several times with saturated aqueous NaHCO₃, then brine, dried over anhydrous Na₂SO4, filtered, and concentrated to afford crude 28 as a Iight yellow-colored solid. Mass spectrum (ESI), m/z 895.3 [(M)+; calcd for C4_eH₅6D4F₂N_eO₈: 895.0].

Scheme XXVII

N-i1S42R-(6,6^l-Difluoro-3¹-{4S-hvdroxv-1-l2S-(2S-methvlamino-propionylamino)-butvrvllpyrrolidiπ-2F?-vl-dg-methvll·-1H,ΓH-[2,2Ίbiindolvl-3-vl-d?-methvl)-4S-hydroxv-pvrrolidine-1carbonvll-propvn-2S-methylamino-propionamide (29): To a solution containing crude 28 (0.15 mmol) in MeOH (20 mL) was added 1 M NaOH (5 mL) at 0 °C. The reaction mixture was degassed and maintained under a nitrogen atmosphère wrapped with aluminum foil. The icebath was removed. After 60 min, the MeOH was removed in vacuo and the residue was diluted with water (20 mL) and extracted with EtOAc (50 mL). The aqueous phase was separated and back-extracted with EtOAc (2 x 50 mL). The combined organic extracts were washed with brine and dried over anhydrous Na2SO4, filtered, and concentrated. The crude product was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, concentrated, and lyophilized to afford 110 mg of 29 (90%, 2 steps) as a flocculent, white-colored solid. ¹H NMR (300 MHz, CDCI3 + ch-MeOH), mixture of rotomers: 011.58 (s, 2H), 7.80 (dd, J - 5.4, 8.7 Hz, 2H), 7.45 (dd, J =2.4, 9.9 Hz, 2H), 6.87 (ddd, J = 2.4, 9.2, 9.2 Hz, 2H), 4.66 (dd, J = 5.7, 7.8 Hz, 2H), 4.60 (br s, 2H), 4.47 (d, J = 7.2 Hz, 2H), 4.00 (dd, J = 4.8, 11.4 Hz, 2H), 3.76 (d, J = 11.4 Hz, 2H), 3.43 (q, J = 6.9 Hz, 2H), 2.55 (s, 6H), 2.19 (d, J = 14.4 Hz, 2H), 1.78-2.02 (m, 8H), 1.46 (d, J = 7.2 Hz, 6H), 1.09 (t, J = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI₃ + d_rMeOH), mixture of rotomers: 0173.6, 171.8, 161.7, 158.6, 137.1, 136.9, 128.1, 128.0, 125.9, 119.8, 119.7, 108.3, 108.2, 107.8, 97.8, 97.5, 70.9, 69.4, 59.0, 56.1, 52.0, 36.3, 35.7, 25.5, 18.5, 9.8 ppm. Mass spectrum (ESI), m/z 811.4 [(M)+; calcd for C₄₂H₅₂D₄F₂N₈O_e: 810.9].

Scheme XXVIII

Acetic acid 5-(3'-(4-acetoxy-1 -[2-(2-dj-methvl-(tert-butoxvcarbonyl)-amino-propionylamino)butvrvl1-pvrrolidin-2-vl-d2-methvl)-6.6'-difluoro-1H.TH42,2'lbiindolyl-3-vl-c/p-methvl)-1-i2-(2-d_gmethvl-(tert-butoxvcarbonvl)-amino-propionvlamino)-butvrvll-pyrrolidin-3-vl ester (30): To a solution containing Boc-N(d_rMe)Ala-OH (17, 83 mg, 0.41 mmol) and HATU (172 mg, 0,45 mmol) in anhydrous NMP (5 mL) at 0 °C was added NMM (0.1 mL, 0.85 mmol) followed by addition of crude 26 (123 mg, 0.17 mmol) in NMP (5 mL). The resulting mixture was allowed to warm to ambient température. After 16 h, the reaction mixture was diluted with dîethyl ether (100 mL) and washed successively with water (50 mL), 1N HCl (2 x 50 mL), saturated aqueous NaHCO₃ (2 x 50 mL), brine, dried over anhydrous Na₂SO₄₁ filtered, concentrated. The crude product was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, concentrated, and lyophilized to afford 160 mg of 30 (85%, 2 steps) as a flocculent, white-colored solid. ¹H NMR (300 MHz, CDCI3), mixture of rotomers; 011.51 (br s, 2H), 7,407.60 (m, 4H), 6.87 (ddd, J = 2.1, 9.0, 9.0 Hz, 2H), 5,47 (t, J = 4.8 Hz, 2H), 4.74 (br s, 2H), 4.65 (q, 7 = 7.2 Hz, 2H), 4.46 (d, 7 = 8.1 Hz, 2H), 4.18 (dd, 7= 3.9, 11.7 Hz, 2H) 3.83 (d, 7= 12.3 Hz, 2H), 2.30 (s, 6H), 2.24 (m, 2H), 2.05 (m, 2H), 1.93 (m, 2H), 1.79 (m, 2H), 1.49 (s, 18H), 1.38 (d, 7 = 6.9 Hz, 6H), 1.02 (t, 7 = 7.2 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI3), mixture of rotomers: □ 175.6, 172.2, 172.1, 170.6, 161.8, 158.7, 137.5, 137.3, 128.5, 128.4, 125.8, 118.7, 118.6, 108.7, 108.4, 108.0, 98.8, 98.4, 81.1, 74.6, 66.1, 59.9, 54.0, 52.1, 33.7, 28.6, 27.5 (br), 25.8, 21.6, 20.9, 14.0, 9.9 ppm; Mass spectrum (ESI), m/z 1101.5 [(M)+; calcd for C_5eH_e6D₁₀F₂NeO₁₂: 1101.3).

Scheme XXIX

Acetic acid 5-(3'-{4-acetoxv-1-[2-(2-c/j-methvlamino-propionvlamino)-butvrvl]-pvrrolidin-2-vl-c/?methvl)-6.6^,-difluoro-1 H, rH-[2,2'lbiindolvl-3-vl-d_rmethyl)-1 -[2-(2-ch-methvlaminopropionvlamino)-butvrvl1-pyrrolidin-3-vl ester (31): A solution containing 30 (160 mg, 0.14 mmol) in DCM (15 mL) was cooled to 0 °C. TFA (5 mL) was added. After 30 min, the reaction mixture was warmed to ambient température and monitored until LC/MS analysis revealed complété conversion of 30 to 31 (ca. 4 h). The solvent was removed in vacuo and the dark, greencolored residue was dissolved in EtOAc (100 mL) and carefully poured onto an aqueous NaHCO₃/ice mixture. The aqueous phase was separated and back-extracted with EtOAc (2 x 20 mL). The combined organic extracts were washed several times with saturated aqueous NaHCO₃, then brine, dried over anhydrous Na₂SO₄, filtered, and concentrated to afford crude 31 as a light yellow-colored solid. Mass spectrum (ESI), m/z 901.5 [(M)+; calcd for C_4BH5oDioF₂N80₈: 901.1].

Scheme XXX aq NaOH. MeOH

N-(1S-f2R-(6.6'-Difluoro-3'-(4S-hvdroxv-1-r2S-(2S-methvlamino-propionylamino)-butvrvllpyrrolidin-2R-vl-c/;>-methvl}-1H,TH-[2.2'lbiindolvl-3-yl-d?-methvl)-4S-hvdroxv-pvrrolidine-1carbonyl1-propYlÎ-2S-methvlamino-propionamide (32): To a solution containing crude 31 (0.14 mmol) in MeOH (20 mL) was added 1 M NaOH (5 mL) at 0 °C. The reaction mixture was degassed and maintained under a nitrogen atmosphère wrapped with aluminum foil. The icebath was removed. After 60 min, the MeOH was removed in vacuo and the residue was diluted with water (20 mL) and extracted with EtOAc (50 mL). The aqueous phase was separated and back-extracted with EtOAc (2 χ 50 mL). The combined organic extracts were washed with brine and dried over anhydrous Na₂SO₄, filtered, and concentrated. The crude product was purified by reverse-phase HPLC (Dynamax 2 C18 column; 10-100% ACN/water containing 0.1% HOAc over 30 min; 40 mL/min). The product-containing fractions were combined, concentrated, and lyophilized to afford 100 mg of 32 (87%, 2 steps) as a flocculent, white-colored solid. ¹H NMR (300 MHz, CDCI3 + cL-MeOH), mixture of rotomers: Π 11.62 (s, 2H), 7.79 (dd, J = 5.4, 8.4 Hz, 2H), 7.47 (dd, J = 2.4, 10.2 Hz, 2H), 6.87 (ddd, J = 2.4, 9.2, 9.2 Hz, 2H), 4.68 (dd, J = 5.4, 7.5 Hz, 2H), 4.58 (m, 2H), 4.45 (d, J = 6.6 Hz, 2H), 3.99 (dd, J = 4.8, 11.1 Hz, 2H), 3.75 (d, J = 11.1 Hz, 2H), 3.19 (q, J = 6.9 Hz, 2H), 2.15 (br d, J = 12 Hz, 2H), 1.78-2.02 (m, 8H), 1.39 (d, J = 6.6 Hz, 6H), 1.07 (t, J = 7.5 Hz, 6H) ppm; ¹³C NMR (75 MHz, CDCI3 + cL-MeOH), mixture of rotomers: 0175.4, 172.0, 161.8, 158.7, 137.1, 137.0, 128.2, 128.0, 126.0, 119.9, 119.7, 108.4, 108.3, 107.9, 98.0, 97.6, 71.0, 60.0, 59.6, 56.2, 51.6, 36.4, 25.8, 19.5, 9.8 ppm; Mass spectrum (ESI), m/z 817.4 [(M)+; calcd for C₄₂H₄eD₁₀F₂N₈O_e: 817.0],

Examples 2, 3, 4, and 5

Compounds tested in Examples 2, 3, 4, and 5 are shown in Table 1.

Table 1

Compound	R5	R
15	-CH2CH3	6-F
2	-CH(CH3)CH3	6-F
3	-R-CH(OH)CH3	6-F
4	-S-CH(OH)CH3	6-F
5	-R-CH(OCH3)CH3	6-F

Example 2A. clAP Dégradation Assay

The concentration inducing dégradation of clAP-1 and clAP-2 by 50% (IC₅₀) for various compounds was determined by monitoring the disappearance of Green Fluorescent Protein (GFP)-signal in A375 cells. Briefly, A375 cell lines expressing GFP-tagged clAP-1 and clAP-2 were generated by transfecting HA2xEGFP-pcDNA3 vector containing either clAP-1 (A375Gc1) orclAP-2 (A375Gc2) coding région. 2x10⁴ of A375Gc1 or A375Gc2 cells were grown in 96-well plate and treated with various concentrations of test compounds for 2 h. After incubation, cells were collected by trypsinization and suspended in 150 pl of DMEM-10% FBS. A total of 10⁴ cells were analyzed using a FACScan (Becton Dickinson). GFP fluorescence was monitored by using an excitation filter at 488 nm and émission was measured with a 530 nm filter. IC₅₀ is defined as the concentration of drug at which 50% of GFP signal was inhibited.

Results of the clAP-1 and -2 dégradation assay are shown in Table 2.

Table 2

Compound	GFP-clAP-1 IC50 (nM)	SD	GFP-clAP-2 IÇ50 fnM)	SD	IC50 Ratio clAP-2/clAP-1
15	27 (n=56)	15	174 (n=61)	100	6.4
2	4 (n=3)	0.6	7 (n=3)	0.8	1.8
3	328 (n=3)	83	674 (n=3)	69	2.1
4	464 (n=12)	112	604 (n=12)	192	1.3
5	10 (n=41)	2	37 (n=38)	19	3.7

These data show that Compound 15 has greater relative potency in degrading clAP-1 relative to clAP-2 when compared to Compounds 2, 3, 4, and 5.

Example 2B. Caspase-3 Derepression Assay

Exponentially growing MDA-MB-231 tumor cells (ATCC) were harvested by trypsinization and collected by centrifugation in a table top centrifuge at 1000xg for 10 minutes at room température. Cell pellet was washed one time by resuspending in 5 mL hypotonie lysis buffer (20 mM HEPES, pH7.5,10 mM KCI, 1.5 mM MgCI₂, 1.0 mM EDTA, 1.0 mM DTT) and recollected by centrifugation. Pellet was next resuspended in 1 volume of hypotonie lysis buffer supplemented with a complété protease inhibitor tablet (Roche) and allowed to swell on ice for 30 minutes. Cells were disrupted by approximately 50 passages through a 27 gauge needle. Lysis was monitored by light microscopy. Lysate was centrifuged at 12000xg for 10 minutes at 4°C to remove membrane fraction, unlysed cells and débris. The soluble fraction was collected for protein concentration détermination and subséquent assay analysis.

The hypotonie lysate (25 pg protein), 50 pg/mL cytochrome c and 10 mM dATP were combined in a microcentrifuge tube to a final volume of 9 pl in hypotonie lysis buffer followed by addition of test compound and incubated for 30 minutes at room temp. Following incubation 50 pl of hypotonie lysis buffer containing 5 pM of pro-fluorescent rhodamine-110₍₂) based caspase-3 substrate zDEVD-R110_(2>was added and fluorescence intensity was monitored over time. Activation of the lysate by addition of cytochrome c and dATP results in apoptosome formation and subséquent activation of caspases-9 and -3. Endogenous XIAP inhibits much of this activity and addition of test compound to the activated lysate results in more caspase activity than is generated by activated lysate alone as measured by increase in fluorescence intensity upon cleavage of zDEVD-R110₍₂₎ by caspase-3. IC₅₀ values were calculated using GraphPad Prism by plotting increase in fluorescence intensity vs. different concentrations of compounds tested and the results are shown in Table 3.

Table 3

Compound	XIAP Caspase-3 Derepression IC50 (nm)	SD
15	24.3 (n=3)	2.0
2	13.4 (n=3)	4.3
3	0.2 (n=3)	0
4	0.35 (n=3)	0.2
5	0.36 (n=3)	0.05

These data show that Compound 15 has a lower potency for antagonizing XIAP function in comparison to Compounds 2, 3, 4, and 5.

Example 3 - Cytotoxicity

SKOV-3 ovarian tumor cell cytotoxicity data were generated substantially as follows. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay is an example of an assay that has been used for measuring cell growth as previously described (Hansen, Μ. B., Nielsen, S. E., and Berg, K. (1989) J. lmmunol. Methods 119, 203-210) and incorporated herein by reference in its entirety. Briefly, SK-OV-3 cells were seeded in 96-well plates in McCoy’s medium containing 10% fêtai bovine sérum albumin (5,000 per well) and incubated overnight at 37°C. The next day, test compounds were added at various concentrations (0.003-10 ΠΜ) and the plates were incubated at 37°C for an additional 72 hrs. This incubation time was optimal for measuring inhibitory effects of different analogs. Fifty microliters of 5mg/mL MTT reagent to each well was added and the plates were incubated at 37°C for 3 hours. At the end of the incubation period, 50 microliters of DMSO was added to each well to dissolve cells and the optical density (OD) ofthe wells was measured using a microplate reader (Victor²1420, Wallac, Finland) at 535 nm. Cell survîval (CS) was calculated by the following équation:

CS = (OD treated well/ mean OD control wells) X 100% The CC₅₀, defined as the drug concentration that results in 50% CS, was derived by calculating the point where the dose-response curve crosses the 50% CS point using GraphPad Prism. These results suggest that Smac mimetics that bind to clAP-1 can be used in the treatment of cancer either as monotherapy or in combination with chemotherapeutics. The results of SKOV3 cytotoxicity assays for the compounds tested in this assay are shown in Table 4.

Table 4

Compound	CCgo (nM)	Std Dev (nM)
15	0.14 (n=76)	0.02
2	0.5 (n=6)	0.1
3	13 (n=4)	4
4	2 (n=23)	0.3
5	0.13 (n=76)	0.1

These data indicate that Compound 15 has équivalent potency to Compound 5 and is more potent than Compounds 2, 3, and 4,

Example 4 - Toxicity

Body weight loss (BWL), mortality and additional toxicity data were generated substantially as follows. Sprague-Dawley rats were dosed daily (QDx4, i.v. bolus slow push) with Compounds 15, 4 and 5. Body weîghts were taken on day 4 and are shown as percent change from day 1. Compounds 4 and 5 were adminîstered at 0.3 mg/Kg, 1 mg/Kg, or 3 mg/Kg; Compound 15 was adminîstered at 1, 5, or 10 mg/Kg.

The results ofthe BWL assay are shown in Fig 1.

Mortality. Compounds 4 and 5 were not tolerated at 3 mg/Kg, and caused the animais to die at this dose. No mortality was observed with Compound 15 at 5 mg/Kg (mortality was observed at 10mg/Kg.)

Clinical Results. There were no clinical signs noted at 1 mg/kg/day with Compound 15 after 4 days of administration. Animais treated with Compound 15 at 5 mg/kg/day exhibited clinical signs similar to Compounds 4 and 5 at 1 mg/kg/day such as lethargy, increased/irregular respiration and increased heart rate. Rats treated with 1 mg/kg Compound 5 exhibited additional clinical observations including déhydration, ungroomed appearance, chromorhinorrhea, alopecia (head), and excessive scratching from days 2 to 4.

Body Weight. At 1 mg/Kg, animais receiving Compounds 4 and 5 lost weight whereas animais receiving Compound 15 at 1 mg/kg/day gained weight. At 5 mg/kg/day with Compound 15, a treatment related mean body weight loss of approximately 8% was noted from day 1 to day 4. A treatment-related mean body weight loss of approximately 4 % and 6 % was noted in animais treated with Compounds 4 and 5, respectively, at 1 mg/kg/day.

Patholoqy. Assessment of anatomie pathology after treatment with Compounds 4 and 5 at 1 mg/kg/day resulted in the following findings. There was marked to severe bone marrow hypocellularity of the erythroid sériés, mild to moderate hypercellularity of the myeloid sériés, and mild to moderate hypertrophy and hyperplasia of megakaryocytes in the tibia and sternum when Compounds 4 and 5 were administered at 1 mg/kg/day. For Compounds 4 and 5 the lungs had dose-related mild to moderate diffuse hypertrophy/hyperplasia of Type 2 pneumocytes which were accompanied by an increase in alveolar macrophages, hypertrophied bronchiolar epithelium, proliferating perivascular mononuclear cells and hypertrophied viscéral pleural cells. ln contrast, assessment of anatomie pathology following treatment with Compound 15 at the same dose (1 mg/kg/day) identified minimal to mild hypocellularity of erythroid cells, minimal to mild hypercellularity of myeloid cells, and minimal Type 2 pneumocyte hypertrophy in the lungs.

The data described above indicate that Compound 15 is approximately 5-fold better tolerated in the rat as compared to Compounds 4 and 5 on a dose per dose basis.

Example 5 - Tumor Volume Réduction and Body Weight Change

MDA-MB-231 xenograft data were generated substantially as follows. MDA-MB-231 human breast tumor cells were injected into the mammary fat pad of female nude mice and dosing initiated twelve days later at an average tumor volume of approximately 148 mm³. No tumor burden was associated with this model based on lack of weight loss or animal morbidity in control groups. Mice were injected subcutaneously into the mammary fat pad with 1x10⁷ cells suspended in 200μΙ of a 1:1 HBSS: Matrigel solution ; injected cells were within nine passages of the original lot. Pre-study tumor volumes were recorded beginning approximately one week prior to the estimated start date. When tumors reached approximately 150 mm³ animais are matched by tumor volume into treatment and control groups and dosîng Initiated (Day 0); mice are tagged and followed individually throughout the experiment. Animais were dosed by weight (0.01 mLpergram; 10ml/Kg).

Beginning on Day 0, animais were observed daily and weighed twice weekly using a digital scale (Ohaus SP601); data including individual and mean gram weights (Mean We ± SD), mean percent weight change versus Day 0 (%vD₀) and mean percent weight change versus prior measurement (%vD._K) were recorded for each group and plotted at study completion.

Beginning on Day 0, tumor dimensions were measured twice weekly by digital caliper (Fowler Ultra-Cal IV) and data including individual and mean estimated tumor volumes (Mean TV ± SEM) recorded for each group; tumor volume was calculated using the formula: TV= width²x length x 0.52. Individual mice reaching the designated study endpoint (an estimated tumor volume of approximately 1 cm³) were assigned a time to endpoint (TTE) value corresponding to that day; the tumor growth delay (TGD) study was concluded once ail mice reach the study endpoint or sixty days following study initiation. At study completion, TGD and %TGD are calculated using the médian TTE (MTTE) value for each treatment (T) group versus control (C) by the formulas: TGD_(ctaySf= T-C and %TGD=T-C/C x100, wherein T-C is the différence between MTTE-Treatment Group and MTTE-Control. Tumored animais not reaching the designated volume endpoint by study completion are considered long-term survivors (LTS) and assigned a TTE value corresponding to the final study day; tumor-free animais are not included in TGD calculations. A Log-rank test is used to détermine statistically significant différences in overall survival expérience between each treated group compared to control. Individual mice reporting a tumor volume ^50% of the Day 0 measurement for two consecutive measurements over a seven day period were considered partial responders (PR). If the PR persisted until study completion, percent tumor régression (%TR) was determined using the formula: %TR= 1-T_f/Ti x100; a mean value was calculated if multiple PR mice occurred in one group. Individual mice lacking palpable tumors (< 4x4 mm² for two consecutive measurements over a seven day period) were classified as complété responders (CR); a CR that persisted until study completion was considered a tumor-free survivor (TFS); TFS animais are excluded from TGD calculations and statistical analysis. Statistical différences in MTTE values between control and treatment groups are compared using a Log Rank test.

Compound 15 was administered by i.p. injection alone at 20, 40 or 60 mg/Kg on a q3dx5 schedule (every three days for 5 cycles). T-C values of 22 days were calculated for these groups, ail of which were found statistically significant compared with control (p=0.005, p<0.0001, or p=0.0001). In the 20 mg/Kg group, 6/10 mice were considered long-term survivors and partial tumor régression was reported in three mice. In the 40 mg/Kg group, 9/10 mice were considered long-term survivors and partial tumor régression was reported in three mice. In the 60 mg/Kg group, 8/10 mice were considered long-term survivors and partial tumor régression was reported in seven mice.

Compound 5 was administered by i.p. injection alone at 15 mg/Kg on a q3dx5 schedule. A T-C value of 21 days was calculated for this group which was found to be statistically significant compared with control (p=0.002). In this group 3/10 mice were considered long-term survivors and partial tumor régression was reported in five mice. The effîcacy of this dose level produced half the number of long term survivors as 20 mg/Kg of Compound 15.

The results of the MDA-MB-231 xenograft assay are shown in Figures 2A and 2B. Compound 15 at 20 mg/Kg had comparable anti-tumor activity to Compound 5 at 15 mg/Kg. Subséquent studies hâve shown that the minimal effective dose of Compound 15 in this model is less than 1 mg/Kg. Weight loss was greater in the mice administered Compound 5 at 15 mg/Kg when compared to the mice dosed with Compound 15 at 20 mg/Kg. Thus, Compound 15 has comparable effîcacy with less toxicity relative to Compound 5 and therefore has an improved therapeutic index.

The compound of Formula 1 is partîcularly well tolerated and well suited for use in a pharmaceutical composition, as well as in a method for treating a proliférative disorder or an autoimmune disorder. In particular, the pharmaceutical composition of the invention for the treatment of a proliférative disorder, which comprises an effective amount of Compound 15 in addition to at least one pharmaceutically acceptable excipient, can improve therapeutic index by reducing toxicities. The reduced toxicities include, e.g., one of, or any combination of one or more of:

• reduced body weight loss, • decreased incidence of mortality, • reduced bone marrow hypocellularity of the erythroid sériés, • reduced hypercellularity of the myeloid sériés, • reduced hypertrophy and hyperplasia of megakaryocytes, • reduced diffuse hypertrophy/hyperplasia of Type 2 pneumocytes • decreased lethargy, • more regular respiration, • lessened increase in heart rate.

The reduced toxicities listed above are those observed in the animais tested. Similar, additional, or different reduced toxicities will be observed in humans. The réductions are relative, e.g., relative to the extent that toxicities would be observed foilowing internai administration of a pharmaceutical composition in which the active pharmaceutical ingrédient is an analog of Compound 15, e.g., one or more ofthe analogs in which R5 is -CH2CH3, CH(CH3)CH3, -R-CH(OH)CH3, -S-CH(OH)CH3, and -R-CH(OCH3)CH3, e.g., at the same dose or at a dose of comparable potency.

It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to

Claims (10)

Claims:

1. A compound having the formula:

2. A pharmaceutical composition comprising the compound having the formula:

wherein R5 is -CH2CH3, or a pharmaceutically acceptable sait thereof, and a pharmaceutically acceptable excipient.

3. The pharmaceutical composition of claim 2 for the treatment of a proliférative disorder.

4. The pharmaceutical composition of claim 2 for the treatment of a cancer.

5 11. Use of Compound 15 or a pharmaceutically acceptable sait thereof in the manufacture of a médicament for treating an autoimmune disease, in a mammal in need thereof, wherein the autoimmune disease is one in which the condition is caused or exacerbated by abnormal régulation of apoptosis and is selected from the group consisting of: systemic lupus erythematosus, psoriasis, and idiopathic thrombocytopénie purpura (Morbus Werlhof), the

5. The pharmaceutical composition of claim 2, 3, or 4 which is a stérile liquid for injection.

6. Use of Compound 15 or a pharmaceutically acceptable sait thereof in the manufacture of a médicament for treating a proliférative disorder in a mammal in need thereof.

7. The use of claim 6 wherein the proliférative disorder is a cancer selected from the group consisting of: lung adenocarcinoma, pancreatic cancer, colon cancer, ovarian cancer, breast cancer, mesothelioma, peripheral neuroma, bladder cancer, glioblastoma, melanoma, adrenocortical carcinoma, AIDS-related lymphoma, anal cancer, bladder cancer, meningioma, glioma, astrocytoma, breast cancer, cervical cancer, chronic myeloproliferative disorders (e.g., chronic lymphocytic leukemia, chronic myelogenous leukemia), colon cancer, endocrine cancers, endométrial cancer, ependymoma, esophageal cancer, Ewing’s sarcoma, extracranial germ cell tumors, extragonadal germ cell tumors, extrahepatic bile duct cancer, gallbladder cancer, gastric cancer, gastrointestinal carcinoid tumors, gestational trophoblastic tumors, hairy cell leukemia, Hodgkin lymphoma, non-Hodgkin lymphoma, hypopharyngeal cancer, intraocular melanoma, islet cell carcinoma, Kaposi sarcoma, laryngeal cancer, leukemia, acute lymphoblastic leukemia, acute myeloid leukemia, lip cancer, oral cavity cancer, liver cancer, male breast cancer, malignant mesothelioma, medulloblastoma, melanoma, Merkel cell carcinoma, metastatic squamous neck cancer, multiple myeloma and other plasma cell neoplasms, mycosis fungoides and the Sezary syndrome, myelodysplastic syndromes, nasopharyngeal cancer, neuroblastoma, non-small cell lung cancer, small cell lung cancer, oropharyngeal cancer, bone cancers, including osteosarcoma and malignant fibrous histiocytoma of bone, ovarian épithélial cancer, ovarian germ cell tumors, ovarian low malignant potential tumors, pancreatic cancer, paranasal sinus cancer, parathyroid cancer, penile cancer, pheochromocytoma, pituitary tumors, prostate cancer, rectal cancer, rénal cell cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, skin cancer, small intestine cancer, soft tissue sarcoma, supratentorial primitive neuroectodermal tumors, pineoblastoma, testicular cancer, thymoma, thymie carcinoma, thyroid cancer, transîtional cell cancer of the rénal pelvis and ureter, uréthral cancer, uterine sarcoma, vaginal cancer, vulvar cancer, and Wilm's tumor and other childhood kidney tumors.

8. Use of Compound 15 or a pharmaceutically acceptable sait thereof in the manufacture of a médicament for inducing apoptosis in a cell comprising contacting the cell with Compound 15.

9. The use of claim 8 wherein the cell is a cancer cell.

10. The use of any one or more of claims 6 through 9 whereby said médicament is administrable in combination with a second cancer therapy selected from radiation, chemotherapy, immunotherapy, photodynamic therapy, and combinations thereof.

10 treatment comprising înternally admînistering to the animal an effective amount of said médicament.