NZ737452B2 - Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food - Google Patents

Abstract

Container B1 comprising a connecting system for providing a fluidic connection with another container B2, where the connecting system comprises at least two connecting arrangements configured to produce the fluidic connection, where the connecting arrangement are configured for mutual opening, with the mouth of one connecting arrangement extending into the other. the mouth of one connecting arrangement extending into the other.

Description

MODULAR SYSTEM OF PHOTOBIOREACTORS FOR ALGAE CULTIVATION DESCRIPTION OBJECT OF THE INVENTION The present invention relates to a modular system of photobioreactors designed for algae cultivation.

The object of the invention is a modular system of closed photobioreactors, intended for indoor operation with LED lighting, uninterrupted and completely automated, with continuous monitoring of process variables, and feedback control of CO2 and nutrients, of water temperature along the entire route of the photobioreactor, allowing maximum use of indoor space where the system is arranged.

BACKGROUND OF THE INVENTION The continued production of algal oil and biomass requires three distinct phases: algae production, product collection and processing for generation of oil and biomass for use, in order to produce biodiesel, cosmetics, pharmaceuticals and other products.

Today algae cultivation is done mainly in outdoor ponds and in photobioreactors made of plastic or glass that may have tubular configurations with diameters ranging mostly from 10 cm to 30 cm.

These systems are generally installed outdoors and operate by pumping water and algae, through a circuit in which carbon dioxide is injected into the tank through which it is pumped. With the action of sunlight algae grow along said circuit. However, these systems have no control over the light spectrums to which algae are subjected, nor on the amount of nutrients, or carbon dioxide required and nor do they control or determine the temperature of the water which the cultivation is grown.

For greater control over light spectra and for proper temperature control, alternatively the photobioreactor can be located indoors where artificial light is applied in the absence of the infrared and ultraviolet spectra which are harmful to algae, and where at least the temperature of the room in which the photobioreactor is located is controlled.

Furthermore and in order to protect the algae, the use of photobioreactors configured by a closed circuit is known, so that, unlike open and external systems, the algae are protected against microorganisms and other harmful agents that have negative effects on algae growth and prevent algae infestation.

The above include the Spanish Patent ES2446640B1 of the same applicant which discloses a photobioreactor for growing algae for indoor use, comprising LED-type lights, and an airtight sealed tubular conduit on which the lights are placed, formed by several tubular sections facing each other and connected together at their ends.

Said photobioreactor further comprises supply nozzles located at the base of the tubular sections, one CO supply line, a nutrient supply line and various temperature conditioned water supply lines, running near and along the route of the tubular conduit, which are connected to said supply nozzles and which are equipped with flow valves This photobioreactor further comprises a plurality of sensors distributed evenly by the tubular conduit to detect the need to incorporate nutrients, of CO and to increase or decrease the water temperature, and a control unit, receiving the signals from the sensors, and which automatically operates the flow valves of the supply lines for supplying an additional amount of CO , nutrients or temperature conditioned water to the various pipe sections, depending on the needs of the cultivation process in view of the signals detected by the sensors and a treatment plant located at the end of the photobioreactor for the final treatment of the product.

Due to the large size of the said photobioreactor, it is necessary to have large indoor spaces to carry out their installation, plus hang elements that support the LED lighting, which makes the use of the indoor space very reduced, with associated increased costs and energy consumption.

The present invention overcomes all the above drawbacks through a modular photobioreactor system that allows maximum use of the indoor space where it is arranged, and a very low power consumption DESCRIPTION OF THE INVENTION The modular system of photobioreactors for algae cultivation of the present invention comprises at least two closed tubular-type photobioreactors, designed to operate continuously in an indoor location, in which storage tanks are located at the front of the modular system and product processing circuits are located at the end of the modular system to produce algae oil and biomass, wherein at least two photobioreactors can be coupled next to each other and / or at different levels.

The modular system of photobioreactors object of this invention is entirely automated to control the growing process in each of the sections of each of the photobioreactor, by data collection and feedback in each of these sections, of the elements and characteristic parameters of the process of cultivating algae, preferably the amount of nutrients, amount of carbon dioxide required, and the water temperature in which the cultivation is grown.

An object of the present invention is therefore the particular arrangement of the modular system of photobioreactors and complementary installations to facilitate measurement and supply of nutrients, carbon dioxide and temperature conditioned water along the sections which define each photobioreactor, as well as the regulation of the above parameters by a control unit.

Each photobioreactor of the modular system of photobioreactors of the present invention comprises a metallic structure which in turn it comprises at least two tubular sections connected by one of its ends, wherein on the inside of said tubular sections the algae culture is arranged and wherein between said tubular sections there is another tubular section comprising LED-type lights and reflector bodies that reflect the light of the LED type lighting and transmit it to the tubular sections in which the cultivation of algae is disposed passing through their side walls .

Moreover, it is noteworthy that the tubular sections of each bioreactor in which the algae cultivation is arranged have a section of trapezoidal configuration, with their lateral sides having a downwardly converging inclination, wherein said sides are made of thermal glass to maximally reduce the heat loss of the water in the interior of each photobioreactor. Each bioreactor is divided into several tubular sections, which can be about 12 meters, and each one of said sections has at its base supply nozzles conveniently distributed that lead to the inlet of CO or water or nutrients, depending on the supply line that is connected. Therefore, the tubular sections of each bioreactor where the LED-type lights and reflector bodies are arranged, have a trapezoidal configuration section, with its lateral sides having an upwardly convergent inclination.

Each photobioreactor therefore has LED-type lights that eliminate the harmful action of infrared and ultraviolet light on algae. These lights provide a spectrum of 400 nm to 700 nm so as to allow optimum and uninterrupted photosynthesis and produce algae every hour.

It is planned that the modular system of photobioreactors also will include in the control unit, means that regulate the activation of the lights, preferably controlling two types of LED, (red and blue), with 2 different spectra, so that it regulates which spectrum should be active at all times and in the most appropriate sections of the photobioreactor, to promote the growth of algae according to their colour.

This modular system of photobioreactors is preferably located in an enclosed building normally kept at room temperature between 15 and 20 °C permanently. In any case the modular system of photobioreactors itself incorporates a control system ensuring at all times optimal water temperature along all sections of each photobioreactor.

The modular system of photobioreactors is connected to a main tank or incubator which stores dechlorinated water, algae and nutrients, mixed with carbon dioxide, which is introduced into each of the bioreactors such that the resulting chemical composition provides a maximum growth of algae.

For this reason provided along the circuit of each of the photobioreactors a series of sensors are arranged that measure the characteristic parameters of the process, for example located every 6 linear meters along the photobioreactor, and which transmit to the control unit, which in a fully automated manner injects carbon dioxide and / or nutrients and / or hot or cold water, depending on the rate of growth of algae in different sections of each photobioreactor, which determines the rate of optimal growth, obtaining thus a higher production than other systems.

The modular system of photobioreactors also has a self-cleaning system by scanning injectors allowing uninterrupted operation which is not possible in other closed circuit systems.

The whole process, from the introduction of the algae into modular system of photobioreactors to the production of biodiesel is done in a fully automated manner.

The size and configuration of the section of the tubular sections, in combination with the location of the supply nozzles at the base of each section, allows for convenient movement of algae resulting in optimum production, wherein the proportion of water / air inside the tubular sections is 70/30 by volume.

Each photobioreactor includes a tubular section with a larger output than the rest of tubular sections, which forms a storage and separation tank for separating oily algae flow having the appropriate size are then directed to the treatment plant, while the rest of the flow is directed back into the main tank.

Furthermore, at the upper part of the tubular sections of each photobioreactor, the part occupied by air, they are exhaust valves to facilitate the outlet of excess oxygen and / or pressure. These valves can be located at the junction between the tubular sections every 12 m.

DESCRIPTION OF THE DRAWINGS To complement the description being made and in order to aid a better understanding of the characteristics of the invention according to a preferred practical embodiment thereof, attached as an integral part of said description, is a set of drawings wherein by way of illustration without limiting the scope of the invention, the following has been represented: Figure 1 shows an elevational view of the modular system of photobioreactors of the present invention in which the arrangement of the tubular sections of the photobioreactors is observed, and the structure that surrounds them and supports both the LED-type lights and the reflectors.

Figure 2 shows a sectional view AA of Figure 1, wherein the storage tanks are also included at the front of the photobioreactors and the product treatment systems located at the end of the photobioreactors.

Figure 3 shows a side view of Figure 2.

Figure 4 shows a view of the detail B of Figure 3, wherein the part of the structure separating a first photobioreactor and a second photobioreactor disposed above the first photobioreactor are shown.

Figure 5 shows an exploded view of the location of the sensors in a section of each of the photobioreactors.

PREFERRED EMBODIMENT OF THE INVENTION Described below is a preferred embodiment of the invention, with reference to FIGS 1 to 5 described above.

Figure 1 shows an elevational a view of a first embodiment of the modular system of photobioreactors of the present invention formed by a first photobioreactor and a second photobioreactor disposed above the first photobioreactor, wherein each photobioreactor comprises a metal structure in turn comprising at least two tubular sections (5) connected by one of their ends, wherein the inside of said tubular sections (5) the algae cultivation is arranged and wherein between said tubular sections (5) another tubular section (25) is arranged comprising LED-type lights (11) and reflector bodies (12) reflecting light from the LED-type lights (11) and transmitting it toward the tubular sections (5) wherein the algae cultivation is arranged, passing through their side walls.

The tubular sections (5) of each bioreactor wherein the algae cultivation is arranged have a section of trapezoidal configuration, with their lateral sides having a downwardly converging inclination to receive light from said reflector bodies (12). These lateral sides are preferably made of thermal glass for better use of light. It is also possible that each of the tubular sections (5) has at least a folding top cover (13) relative to a lateral side permitting access therein.

Therefore the tubular sections (25) of each bioreactor in which the LED-type lights (11) and the reflector bodies (12) are arranged, have a section of trapezoidal configuration, with its lateral sides having an upwardly convergent inclination.

In this first embodiment, the modular system of photobioreactors further comprises a drainage structure disposed between the metal structure of the first photobioreactor and the metal structure of the second photobioreactor, wherein said drainage structure comprises a perforated top wall (20) and a lower wall (21) with a drainage hole (22) so that the water flows through the perforated top wall (20) to the drainage hole (22) in case of water spilling from the second photobioreactor disposed above the first photobioreactor.

In a second embodiment, the photobioreactors are arranged side by side.

In a third embodiment, a combination of the first and second embodiment, the system comprises at least three photobioreactors which include a first photobioreactor, a second and a third photobioreactor photobioreactor, wherein the third bioreactor is arranged beside the first bioreactor and wherein the second bioreactor is disposed above or below the first or third photobioreactor.

The modular system of photobioreactors is arranged within a facility in which there is the main tank or incubator (1) containing dechlorinated water, algae and nutrient, saturated with a mixture of CO , CO tanks (2) and a nutrient tanks (3), in correspondence with the inlet of each photobioreactor and a treatment plant (4) located at the end of the photobioreactor for the final treatment of the product.

The tubular sections (5) wherein the algae cultivation is arranged include at their base at least one supply nozzle (6), and comprising a CO supply line (7), a nutrient supply line (8) and several temperature conditioned water supply lines (9), which are distributed near and along the tubular conduit, which are connected to the said supply nozzles (6), as seen in greater detail in Figure 2 and that are equipped with flow valves.

Also, each photobioreactor comprises evenly distributed tubular sections (5) wherein the algae cultivation is arranged, and a series of nutrient sensors (15) detecting the need to incorporate nutrients, CO sensors (16), temperature sensors ( 17) which detect the need to increase or decrease the water temperature and pH sensors (18), all shown in Figure 5, and has a control unit, receiving the signals from the sensors, and automatically acting on the flow valves of the supply lines (7, 8, 9) for supplying an additional amount of CO , nutrients or temperature conditioned water to different tubular sections (5), depending on the process needs in view of the signals detected by the sensors.

As seen in Figure 2, the supply line of CO (7) is connected and feeds the CO tank (2) and the supply line of nutrients (8) is connected and feeds the nutrient tank (3).

Furthermore, each of the temperature conditioned water supply lines (9) extend in correspondence with different sectors of each bioreactor. Here they are distributed in correspondence with each parallel longitudinal sector of the bioreactor, as shown in Figure 2, to establish an independent temperature regulation of each of said sectors each of said temperature conditioned water supply lines (9) being connected to a separate heating unit (10), normally located, as shown in Figure 2, at the end of each sector positioned opposite the location of the photobioreactor inlet tubular section.

The initial tubular section (5) of each photobioreactor, which occupies the inlet position and which is reached by the product that houses the main tank (1) has a series of openings (14) evenly distributed on the front face, here being 6 in number, designed to receive as many conduits that carry product from the main tank (1) to each photobioreactor, thus facilitating uniform inlet and distribution of the product flow from the main tank (1) in each photobioreactor.

Claims (15)

1. A modular system of photobioreactors for algae cultivation, wherein the system comprises at least two photobioreactors each of which comprises a metallic structure in turn comprising at least two tubular sections connected by one of its ends, wherein inside said tubular sections the algae cultivation is arranged and wherein, between said tubular sections, the system comprises LED-type lights and reflector bodies that reflect the light from the LED-type lighting and transmit it to the tubular sections in which the cultivation of algae is disposed, passing through their side walls, wherein the system further comprises another tubular section disposed between said tubular sections, wherein the other tubular section comprises the LED-type lights and the reflector bodies.

2. The modular system of photobioreactors for algae cultivation according to claim 1, wherein the tubular sections of each bioreactor in which the algae cultivation is arranged have a section of trapezoidal configuration, with its lateral sides having a downwardly convergent inclination to receive light from the reflector bodies, and the tubular sections of each bioreactor in which the LED- type lights and the reflector bodies are arranged have a section of trapezoidal configuration, with its lateral sides having an upwardly convergent inclination.

3. The modular system of photobioreactors for algae cultivation according to either claim 1 or 2, wherein the system comprises at least two photobioreactors including a first photobioreactor and a second photobioreactor disposed above the first photobioreactor.

4. The modular system of photobioreactors for algae cultivation according to either claim 1 or 2, wherein the system comprises at least two photobioreactors including a first photobioreactor and a second photobioreactor disposed beside the first photobioreactor.

5. The modular system of photobioreactors for algae cultivation according to either claim 1 or 2, wherein the system comprises at least three photobioreactors including a first photobioreactor, a second photobioreactor and a third photobioreactor, wherein the third photobioreactor is disposed next to the first bioreactor and wherein the second bioreactor is disposed above or below the first or the third photobioreactor.

6. The modular system of photobioreactors for algae cultivation according to either claim 3 or 5, wherein the system further comprises a drainage structure disposed between the metal structure of the first photobioreactor and the metal structure of the second photobioreactor, wherein said drainage structure comprises a perforated top wall and a lower wall with a drainage hole so that the water flows through the perforated top wall to the drainage hole in case of spillage of water from the second bioreactor disposed above the first photobioreactor.

7. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein the system further comprises: supply nozzles located at the base of the tubular sections in which the algae cultivation is arranged, a CO supply line, a nutrient supply line and several temperature conditioned water supply lines, which are distributed near and along the route of the tubular sections in which the algae cultivation is disposed, which are connected to said supply nozzles and are equipped with flow valves, a series of nutrient sensors detecting the need to incorporate nutrients, CO sensors and temperature sensors detecting the need to raise or lower the water temperature, and a control unit which receives the signals from the sensors and which automatically operates the flow valves of the supply lines (7, 8, 9) for supplying an additional amount of CO , nutrients or temperature conditioned water to different tubular sections in which the algae cultivation is arranged, according to the needs of the cultivation process in view of the signals detected by the sensors.

8. The modular system of photobioreactors for algae cultivation according to claim 7, wherein the system further comprises a series of pH sensors.

9. The modular system of photobioreactors for algae cultivation according to claim 7, wherein the temperature conditioned water supply lines extend separately in correspondence with the tubular sections in which the algae cultivation is arranged in each photobioreactor.

10. The modular system of photobioreactors for algae cultivation according to claim 9, wherein each of the temperature conditioned water supply lines is connected to a heating unit.

11. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein each of the tubular sections in which the algae cultivation of each photobioreactor is arranged, has at least a folding top cover relative to a lateral side that allows access to its interior.

12. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein each photobioreactor has an initial tubular section in which the algae cultivation is arranged and which is equipped on its front face with a series of evenly distributed openings, intended to receive a corresponding number of conduits carrying the product being introduced into the photobioreactor.

13. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein each LED-type light comprises two types of LED, red and blue, with two different spectra, and the control unit has means for regulating the activation of these lights of two types of LEDs in different tubular sections in which the cultivation of algae is arranged.

14. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein each photobioreactor incorporates an output tubular section in which the algae cultivation is arranged, being larger than the other tubular sections in which the algae cultivation is arranged, which constitutes a storage and separation tank with upper outlets for separating the flow of oily algae, and with several lower outlets, with a greater flow area than the upper outlets.

15. The modular system of photobioreactors for algae cultivation according to any of the preceding claims, wherein each photobioreactor comprises, in its upper part, evenly distributed exhaust valves to facilitate the exit of excess oxygen and / or pressure.