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NZ504725A - Flavivirus expression and delivery system - Google Patents

Flavivirus expression and delivery system

Info

Publication number
NZ504725A
NZ504725A NZ504725A NZ50472598A NZ504725A NZ 504725 A NZ504725 A NZ 504725A NZ 504725 A NZ504725 A NZ 504725A NZ 50472598 A NZ50472598 A NZ 50472598A NZ 504725 A NZ504725 A NZ 504725A
Authority
NZ
New Zealand
Prior art keywords
flavivirus
vector
self
replicating
utr
Prior art date
Application number
NZ504725A
Inventor
Edwin G Westaway
Alexander A Khromykh
Andrei Varnavski
Original Assignee
Crown In The Right Of The Quee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPP0627A external-priority patent/AUPP062797A0/en
Priority claimed from AUPP6096A external-priority patent/AUPP609698A0/en
Application filed by Crown In The Right Of The Quee filed Critical Crown In The Right Of The Quee
Publication of NZ504725A publication Critical patent/NZ504725A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2770/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A gene expression system comprising: a) a self-replicating expression vector of flavivirus origin which includes the flavivirus 5' untranslated region (UTR), at least a portion of the 5' coding region for flavivirus core protein, the nucleotide sequence coding for the flavivirus non-structural proteins, and the complete or most of the 3'-terminal sequence of the flavivirus 3'UTR, required for self-replication of flavivirus genomic material, which vector is adapted to receive at least a nucleotide sequence without disrupting its replication capabilities; and b) at least a second vector that is capable of expressing flavivirus structural protein(s) and any other proteins required for packaging of the self-replicating expression vector into flavivirus viral particles which vector is engineered to prevent recombination with the self-replicating vector when in its presence.
NZ504725A 1997-11-28 1998-11-30 Flavivirus expression and delivery system NZ504725A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPP0627A AUPP062797A0 (en) 1997-11-28 1997-11-28 Flavivirus expression system
AUPP6096A AUPP609698A0 (en) 1998-09-23 1998-09-23 Flavivirus expression system
PCT/AU1998/000993 WO1999028487A1 (en) 1997-11-28 1998-11-30 Flavivirus expression and delivery system

Publications (1)

Publication Number Publication Date
NZ504725A true NZ504725A (en) 2003-02-28

Family

ID=25645666

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ504725A NZ504725A (en) 1997-11-28 1998-11-30 Flavivirus expression and delivery system

Country Status (5)

Country Link
EP (1) EP1034290A4 (en)
JP (2) JP2002500003A (en)
CA (1) CA2311395C (en)
NZ (1) NZ504725A (en)
WO (1) WO1999028487A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1035205A1 (en) * 1999-03-08 2000-09-13 Stichting Dienst Landbouwkundig Onderzoek Non-spreading pestivirus
US6589531B1 (en) 2000-01-21 2003-07-08 The Regents Of The University Of California Recombinant yellow fever virus and method of use thereof
EP1195438A1 (en) 2000-10-06 2002-04-10 Rijksuniversiteit te Groningen Genetic immunisation against cervical carcinoma
KR20020059856A (en) * 2001-01-08 2002-07-16 김철중 Preparation of hiv-like particles
WO2002072803A2 (en) 2001-03-09 2002-09-19 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Subgenomic replicons of the flavivirus dengue
GB2372991B (en) * 2001-03-09 2004-11-17 Fiocruz Fundacao Oswaldo Cruz Flavivirus expression vector
FR2829149B1 (en) * 2001-09-06 2004-09-24 Pasteur Institut NEUROVIRULENT STRAIN OF WEST NILE VIRUS AND ITS APPLICATIONS
US7871814B2 (en) * 2001-05-10 2011-01-18 The Regents Of The University Of California Recombinant bicistronic flaviviruses and methods of use thereof
JP2005510244A (en) * 2001-11-26 2005-04-21 ザ・ユニヴァーシティ・オヴ・クイーンズランド Flavivirus vaccine delivery system
EP1593738A4 (en) * 2003-01-30 2006-05-31 Shanghai Tengen Biomedical Co Virus-like particle vaccine containing dengue virus recombinant replicon as its carrier
CN103088038B (en) * 2004-07-12 2015-06-24 美国天甲生物医药有限公司 Flavivirus vaccine
BRPI0504945B8 (en) * 2005-10-31 2022-08-02 Fundacao Oswaldo Cruz METHOD FOR PRODUCTION OF RECOMBINANT FLAVIVIRUS CONTAINING NUCLEOTIDE SEQUENCES ENCODING A HETEROLOGOUS PROTEIN, DNA CONSTRUCT, FLAVIVIRUS, AND, VACCINE COMPOSITION TO IMMUNIZE AGAINST FLAVIVIRUS AND/OR OTHER PATHOGENS.
MY151062A (en) 2006-02-27 2014-03-31 Univ Texas Pseudoinfectious flavivirus and uses thereof
US8815564B2 (en) 2008-03-14 2014-08-26 Sanofi Pasteur Biologics, Llc Replication-defective flavivirus vaccines and vaccine vectors
ES2586580T3 (en) * 2010-07-06 2016-10-17 Glaxosmithkline Biologicals Sa Immunization of large mammals with low doses of RNA
HUE026646T2 (en) 2010-07-06 2016-07-28 Glaxosmithkline Biologicals Sa Liposomes with lipids having an advantageous pka-value for rna delivery
ES2646669T3 (en) 2010-07-06 2017-12-14 Glaxosmithkline Biologicals Sa Procedures for increasing an immune response by providing RNA
DK4226941T3 (en) 2010-08-31 2024-12-02 Glaxosmithkline Biologicals Sa PEGYLATED LIPOSOMES FOR DELIVERY OF IMMUNOGEN-ENCODING RNA
KR102266691B1 (en) 2010-10-11 2021-06-23 노파르티스 아게 Antigen delivery platforms
US10494421B2 (en) 2014-02-10 2019-12-03 Univercells Nv System, apparatus and method for biomolecules production
EP3061826A1 (en) * 2015-02-27 2016-08-31 Novartis AG Flavivirus replicons

Also Published As

Publication number Publication date
EP1034290A1 (en) 2000-09-13
JP2002500003A (en) 2002-01-08
CA2311395A1 (en) 1999-06-10
CA2311395C (en) 2006-11-28
WO1999028487A1 (en) 1999-06-10
JP2008113666A (en) 2008-05-22
EP1034290A4 (en) 2004-09-15

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Legal Events

Date Code Title Description
PSEA Patent sealed
RENW Renewal (renewal fees accepted)
ASS Change of ownership

Owner name: THE COUNCIL OF THE QUEENSLAND INSTITUTE OF MED, AU

Free format text: OLD OWNER(S): THE CROWN IN THE RIGHT OF THE QUEENSLAND DEPARTMENT OF HEALTH

RENW Renewal (renewal fees accepted)
ASS Change of ownership

Owner name: REPLIKUN BIOTECH PTY LTD, AU

Free format text: OLD OWNER(S): THE COUNCIL OF THE QUEENSLAND INSTITUTE OF MEDICAL RESEARCH

RENW Renewal (renewal fees accepted)