NL2038369B1

NL2038369B1 - A composition of natural kiwifruit powder and postbiotics and its use in constipation improvement

Info

Publication number: NL2038369B1
Application number: NL2038369A
Authority: NL
Inventors: Stepanko Olga
Original assignee: Sunbird Company Ltd
Priority date: 2024-07-31
Filing date: 2024-07-31
Publication date: 2025-03-06

Abstract

Disclosed is a composition of natural kiwifruit powder and postbiotics, and its use in consti- 5 pation improvement, wherein the composition further includes probiotics, prebiotics, and sugar alcohol components, and can improve constipation, increase the frequency of defecation in constipated individuals, soften feces without loose stools, and reduce the incidence of loose stools during defecation.

Description

A COMPOSITION OF NATURAL KIWIFRUIT POWDER AND POSTBIOTICS AND ITS USE IN

CONSTIPATION IMPROVEMENT

TECHNICAL FIELD

The present invention belongs to the field of dietary nutritional supplements and relates to a composition of natural kiwifruit powder and postbiotics, as well as its use in constipation improve- ment, wherein the composition further includes probiotics, prebiotics, and sugar alcohol compo- nents, and can improve constipation, increase the frequency of defecation in constipated individ- uals, soften feces without loose stools, and reduce the incidence of loose stools during defecation.

BACKGROUND

Constipation relates to a decrease in the frequency of bowel movements or difficulty in defe- cation, hard stools, or a feeling of incomplete rectal emptying after defecation, which affects peo- ple's quality of life.

Laxatives (such as volumetric laxatives, osmotic laxatives, irritant laxatives, lubricating laxa- tives) and prokinetic drugs are generally used for traditional medication treatment. These drugs achieve laxative effects through different mechanisms, but long-term use may cause side effects such as electrolyte imbalance, abdominal pain, etc., and in addition, some may have adverse impact on the gut microbiota, further exacerbating the imbalance of gut microbiota.

Therefore, the urgent technical issue to be addressed is to provide a safe and effective die- tary supplement.

SUMMARY

The purpose of the present invention is to provide a composition containing natural kiwifruit powder and postbiotics to address the shortcomings of the prior art.

The present invention provides a composition of natural kiwifruit powder and postbiotics, wherein the content of postbiotics is 1x108~1x10" cell units/serving, for example, 1x108, 5 x 108 1x 10° 5x10°% 1 x 1079, 5x10" 1 x 10" cell units/serving.

Kiwifruit powder, rich in dietary fiber and various nutrients, can promote intestinal peristalsis, increase fecal volume, and help alleviate constipation.

Postbiotics are active products or cellular components produced by probiotics during metab- olism, including short chain fatty acids, cell wall components, extracellular polysaccharides, etc.

Postbiotics do not contain live bacteria like probiotics, but still have significant health benefits, such as regulating the intestinal environment, enhancing intestinal barrier function, and modulat- ing the immune system.

Stability is a significant advantage of postbiotics, and representative components, such as fatty acids, are crucial for the ability of postbiotics against acids, bases, and heat. Therefore, screened postbiotics are superior to the native live bacteria in enhancing immune function, and can maintain high physiological activity even after high temperature treatment or gastrointestinal digestion fluid treatment. In intestinal environments where probiotics are difficult to survive and colonize, postbiotics can provide similar or even enhanced effects as probiotics.

In further, the strains of postbiotics source in the present invention include but are not limited to Lactobacillus plantarum, Bifidobacterium longum, Lactobacillus delbrueckii, Lactobacillus brevis, Lactobacillus cellulosis, Lactobacillus acidophilus, Bifidobacterium adolescentis Bifidobac- terium bifidum, Bifidobacterium longum subsp. infantis, Streptococcus lactiae, Bacillus subtilis,

Clostridium butyricum, Lactobacillus casei, Lactobacillus helveticus, Lactobacillus gassen, Lac- tobacillus reuteri, and Akkermansia muciniphila.

The composition including natural kiwifruit powder and postbiotics according to the present invention further includes probiotics.

Consuming sufficient amounts of probiotics, a type of living microorganism, can provide health benefits to the host, since they maintain the balance of gut microbiota through various mechanisms, such as competitive exclusion of harmful bacteria, production of antibacterial sub- stances, and regulation of immune system. The main characteristic of probiotics is activity, and probiotics need to be colonized in the intestine for functioning purpose.

In further, the probiotic content in the present invention is 1 x 108 ~ 1 x 10" CFU/serving, for example, 1 x 10%, 5x 103, 1 x 10°, 5x 10° 1 x 1019, 5x 10%, and 1 x 10" CFU/serving.

In further, the probiotics in the prevent invention include but are not limited to: Lactobacifius acidophilus, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus celluloses, Lactobacillus delbrueckii, Lactobacillus lactis, Lactobacillus helveticus, Lactobacillus gasseri, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis,

Bifidobacterium animalis, Streptococcus lactiae, Bacillus subtilis, Clostridium butyricum, Saccha- romyces boulardii, Lactobacillus reuteri, and Akmansia muciniphila.

The composition including natural kiwifruit powder and postbiotics according to the present invention further includes probiotics, and in further, prebiotics.

Prebiotics mainly provide food for probiotics to promote their growth and activity, while probi- otics indirectly advance the production of postbiotics by promoting the growth and metabolic ac- tivity of prebiotics.

In further, the content of prebiotics in the present invention is 1.5 - 10 g/serving, preferably 2 - 4 g/serving, for example, 1.5,2,2.5,3,3.5,4,45,5,5.5,6,6.5,7,7.5, 8, 8.5, 9, 9.5, 10g per serving.

In further, the prebiotics described in the present invention include but are not limited to oli- gosaccharides, inulin, resistant starch, pectin, arabinoxylan, and B-glucan. In further, the oligo- saccharides described in the present invention include but are not limited to galactooligosaccha- rides, xylooligosaccharides, fructooligosaccharides, isomaltooligosaccharides, lactulose, and stachyose.

The present invention provides a combination of natural kiwi fruit powder and postbiotics,

further including sugar alcohol. In further, the sugar alcohols described in the present invention include but are not limited to sorbitol, lactitol, xylitol, mannitol, and erythritol. Furthermore, the sugar alcohol content of the present invention is 1.5-10 g/part, preferably 2-4 g/part. For example, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10g/serving.

The present invention provides a combination of natural kiwifruit powder and postbiotics, in further, wherein the kiwifruit powder is prepared by the following steps: (1) choosing natural kiwifruit as the raw material and peeling the kiwifruit; (2) cutting the peeled kiwifruit into slices, chunks, or cubes, and drying them; (3) crushing and sieving dried kiwifruit into powder.

In further, for the natural kiwifruit in the preparation step (1), pest-, disease- and mechanical damage-free kiwifruit with consistent maturity is selected as raw materials and, preferably, one or more of the following is selected: Golden kiwifruit, Hayward, SunGold, Qinmei, Xuxiang, Cuixiang,

Hongyang, and Actinidia chinensis Planch. .

In further, for the natural kiwifruit in the preparation step (1), the peeled kiwifruit is soaked in 45°C B-cyclodextrin solution with a mass fraction of 2% to remove the bitterness.

In further, for the methods for peeling natural kiwifruit in preparation step (1), the kiwifruit can be peeled off by a mechanical peeler, or by heat such as hot water soaking or steam treatment.

In further, in the preparation step (2), the peeled kiwifruit slices, chunks, or cubes have a thickness of 2 to 5 millimeters, 5 to 10 millimeters, and 3 to 8 millimeters, respectively.

In further, the drying process in preparation step (2) is a crucial step, in which moisture is removed while nutritional components such as dietary fiber and polyphenols are maintained.

Freeze drying, vacuum drying, hot air drying, microwave drying and spray drying can be used.

In further, in the preparation step (2), the freeze-drying temperature is between -20 °C and - 50 °C, the pressure is 1-10 mbar, and the drying time is 2-24 hours. The nutritional components and flavor of kiwifruit can be well maintained under low temperature and low pressure.

In further, in the preparation step (2), vacuum drying is performed at a pressure of 1-100 mbar, a temperature of 40°C to 60°C, and a drying time of 2-24 hours. Vacuum drying under low pressure can reduce the damage of heat sensitive components and maintain the color and nutri- tional content of kiwifruit.

In further, in the preparation step (2), hot air drying is performed by utilizing the flow of hot air to remove moisture, with a temperature between 40°C and 70°C, a wind speed of 1 - 5 m/s, and a drying time of 2 - 24 hours.

In further, in the preparation step (2), microwave drying is performed by utilizing microwave energy to directly heat the material, with a microwave power of 100 W to 2000 Wand a microwave drying time of 5 minutes to 8 hours.

In further, for the spray drying in the preparation step (2), kiwifruit needs to be further stirred and chopped into slurry and the slurry, after being atomized through the nozzle, is dried rapidly by hot air in the drying chamber. The inlet air temperature is between 150°C and 200°C, the outlet air temperature is between 80°C and 80°C, and the drying time is 1 — 30 min.

In further, in the preparation step (3), a pulverizer is used for pulverization, wherein the pul- verizer includes but is not limited to hammer mills, ball mills, and air flow mills.

In further, in the preparation step (3), uniform particle size is achieved through sieving, and the mesh size is in the range of 40-120 mesh, such as 40, 60, 80, 100, and 120 mesh.

The present invention provides a composition of natural kiwi fruit powder and postbiotics , and in further, wherein the kiwifruit powder has a mass of 0.001 - 5 g/serving, for example, 0.001, 0.002, 0.003, 0.005, 0.008, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 0.7, 0.8, 0.9, 1, 1.2, 1.4, 1.6, 1.8, 2,2.2,2.4, 2.8, 2.8, 3, 3.2, 3.4, 3.8, 3.8, 4, 4.2, 4.4, 4.8, 4.8, 5 g/serving.

The present invention provides a composition of natural kiwi fruit powder and postbiotics, and in further, wherein the composition includes probiotics, and further prebiotic, and the composition is prepared in a form that is easily accepted by the human body, including but not limited to tablets, capsules, powders, oral liquids, jellies, and gummies.

The present invention provides a composition of natural kiwi fruit powder and postbiotics, and in further, wherein the composition includes probiotics, and further prebiotics, and can not only alleviate constipation symptoms but also fundamentally improve intestinal health. The composi- tion can be used to improve constipation, increase the frequency of bowel movements in consti- pated individuals, soften feces without loose stools, and reduce the incidence of loose stools during bowel movements.

Definition of Terms

The “postbiotics” used herein refer to the components obtained after probiotics are pro- cessed, including bacterial cells (such as fatty acids, phosphoric acids, peptidoglycans, cell sur- face proteins, polysaccharides, cell membrane proteins, extracellular polysaccharides, etc.) and their metabolites (such as vitamins, lipids, enzymes, proteins, peptides, organic acids, short chain fatty acids, intracellular polysaccharides, etc.).

The term “probiotics” used herein refers to live microorganisms that, when consumed in suf- ficient amounts, can benefit the health of the host. Probiotics mainly colonize the human gut, but also exist on the oral cavity, vagina, and other mucosal surfaces.

The “prebiotics” used herein mainly provide food for probiotics to promote their growth and activity.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

In order to facilitate the understanding of those skilled in the art, the present invention will be further elaborated in conjunction with the embodiments below, and the content mentioned in the embodiments is not a limitation of the present invention.

Example 1

A composition including kiwifruit powder and Lactobacillus plantarum postbiotics, wherein the kiwifruit powder content is 0.002 g/serving, and the Lactobacillus plantarum is postbiotics, with an added amount of 1 x 10° cells/serving.

Example 2

A composition including kiwifruit powder and Bifidobacterium longum postbiotics, wherein the kiwifruit powder content is 0.002 g/serving, and the Bifidobacterium longum is postbiotics, with an 5 added amount of 1 x 10° cells/serving.

Example 3

A composition including kiwifruit powder, Lactobacillus plantarum postbiotics and Bifidobac- terium longum postbiotics, wherein the kiwifruit powder content is 0.002 g/serving, and the Lac- tobacillus plantarum and Bifidobacterium longum are postbiotics, with an added amount of 1 x 10° cells/serving.

Example 4

A composition including kiwifruit powder and probiotics, wherein the kiwifruit powder content is 0.1 g/serving, and the probiotics are Bifidobacterium animalis and Lactobacillus rhamnosus, with a total added amount of 1 x 1019 CFU/serving.

Example 5

A composition including kiwifruit powder, probiotics, and prebiotics, wherein the kiwifruit pow- der content is 0.1 g/serving, the probiotics are Bifidobacterium animalis and Lactobacillus rham- nosus, with a total added amount of 1 x 1019 CFU/serving, and the prebiotics is oligofructose, with an added amount of 2.5 g/serving.

Example 6

A composition including kiwifruit powder, postbiotics, probiotics, and prebiotics, wherein the kiwifruit powder content is 0.1g/serving, the Lactobacillus plantarum and Bifidobacterium longum are postbiotics, with an added amount of 2 x 10° cells/serving, the probiotics are Bifidobacterium animalis and Lactobacillus rhamnosus, with a total added amount of 2x10° CFU/serving, and the prebiotics is polyglucose, with an added amount of 2.5 g/serving.

Example 7 Preparation of jelly preparations

Weighed an appropriate amount of jelly powder and mixed it with kiwifruit powder in a specific ratio, added the mixed jelly base to boiling water and stirred continuously with a mixer until all ingredients were completely dissolved, and then cooled to about 40 - 50°C. Added a certain pro- portion of postbiotics powder (Lactobacillus plantarum and Bifidobacterium longum postbiotics), stirred evenly, heated the mixed jelly solution in a hot water bath at 80 - 85°C for 5 minutes, for pasteurization, cooled to room temperature, and then poured into a sterile jelly mold. Place the filled mold in a refrigeration device and cooled to below 4 °C until the jelly was completely solidi- fied. After about 6 hours, removed it from the mold for aseptic packaging and get the jelly prepa- ration.

Example 8 Preparation of capsule preparations

Weighed kiwifruit powder, probiotics (Bifidobacterium animalis, Lactobacillus rhamnosus), and prebiotics (oligofructose) according to the formula, poured them into a mixer for mixing, and dried the mixed materials through a fluidized bed dryer at 40 - 60 °C for 3 hours, ensuring uniform drying. Cooled them to room temperature and delivered the materials into a capsule filling ma- chine for filling.

Example 9 Evaluation of the effect of intestine moisturization and bowel movement improve- ment through human feeding trial

Human feeding scheme:

According to the inclusion criteria (functional constipation and habitual constipation: no more than 5 times of bowel movement per week and conforming to Bristol stool symptoms Ill and IV) and exclusion criteria (unable to take the test sample as prescribed; experiencing pain during defecation; having serious organic lesions such as intestinal obstruction, colitis, enteritis, inflam- matory bowel disease causing constipation; having recently experienced acute gastrointestinal diseases; pregnant and menstruating women; taking laxatives in the short term), 30 composite standard subjects were screened and divided into 6 groups, 5 each group, and taken the products prepared in Examples 1 - 6 respectively; the experiment was conducted using in self controlled manner. During the trial period, the original dietary habits and habits were not changed.

Effect assessment

Record the bowel movements of each group of subjects one week before and during taking each sample. According to the Bristol fecal trait classification system, fecal traits are classified into grades | to V (corresponding to 1 to 5 points respectively}, and the fecal trait score is calcu- lated. The average score of defecation status is obtained by dividing the sum of defecation status scores within a week by the total number of defecation times within a week. The proportion of people experiencing pain during bowel movements (number of subjects experiencing pain within 7 days/total number of subjects) is synchronously recorded.

The defecation situation is shown in table 1 below.

Table 1

Per capita frequency of bowel move- Per capita defecation characteristics/ ments per week/

Before test After test Before test After test

Games | 28 | ee | a | 4

The results reveal that the combination of kiwi powder and postbiotics can increase the fre- quency of bowel movements in constipated patients. When combined with probiotics and prebi- otics, the combination can improve the stool state of constipated patients to a certain extent, thereby reducing the difficulty of defecation and abdominal pain during defecation.

Persons skilled in the art can understand that, unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as those generally understood by the persons having ordinary skill in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with those in the context of prior art, and unless specifically defined as here, they will not be interpreted with idealized or overly formal meanings.

It should be understood that the detailed description of the technical solution of the present invention with the help of preferred embodiments is illustrative rather than restrictive. The persons having ordinary skill in the art may modify the technical solutions described in each embodiment based on reading the present invention specification, or equivalently replace some of the technical features, and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the embodiments of the present inven- tion.

Claims

CONCLUSIONS

1. A composition of natural kiwifruit powder and postbiotics, wherein the content of postbiotics is 1 x 108 ~ 1 x 10" cell units/portion, and the kiwifruit powder is extracted by a method comprising the following steps: (1) selecting kiwifruit free from pests, diseases and mechanical damage and having constant maturity as raw materials, and removing the bitter taste by soaking the peeled kiwifruit in a β-cyclodextrin solution with a mass fraction of 2% at 45°C; (2) cutting the peeled kiwifruit into 5 mm thick slices and vacuum-drying with microwaves at a microwave power of 1,000 W for 20 minutes, drying at 60°C for 4 hours, followed by natural cooling; and (3) placing the dried fruit slices in a high-speed mill to grind them into powder, sieving and obtaining powdered kiwifruit.

The composition according to claim 1, wherein the postbiotic strains comprise as source one or more of the following: Lactobacillus plantarum, Bifidobacterium longum, Lactobacillus delbrueckii, Lactobacillus brevis, Lactobacillus cellulosis, Lactobacillus acidophilus, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum subsp. infantis, Streptococcus laciae, Bacillus subtilis, Clostridium butyricum, Lactobacillus casei, Lactobacillus helveticus, Lactobacillus gasser, Lactobacillus reuteri and Akkermansia muciniphila.

3. The composition of claim 1, further comprising probiotics.

4. The composition according to claim 3, wherein the probiotic content is 1 x 103 ~ 1 x 10" CFU/serving.

5. The composition according to claim 3, wherein the probiotics are selected from one or more of the following: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus celluloses, Lactobacillus delbrueckii, Lactobacillus lactis, Lactobacillus helveticus, Lactobacillus gasseri, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium animalis, Streptococcus lactiae, Bacillus subtilis, Clostridium butyricum, Saccharomyces boulardii, Lactobacillus reuteri and Akmansia muciniphila.

6. The composition of claim 3, further comprising prebiotics.

7. The composition according to claim 6, wherein the prebiotics are selected from one or more of the following: oligosaccharides, inulin, resistant starch, pectin, arabinoxylan and β-glucan.

8. The composition according to claim 7, wherein the oligosaccharides are selected from one or more of the following: galacto-oligosaccharides, xylo-oligosaccharides, fructo-oligosaccharides, isomalto-oligosaccharides, lactulose and stachyose.

9. The composition according to claim 6, wherein the prebiotic content is 1.5 - 10 g/serving.

10. The composition of claim 1, further comprising sugar alcohol.

11. The composition of claim 10, wherein the sugar alcohol is selected from one or more of the following: sorbitol, lactitol, xylitol, mannitol and erythritol.

12. The composition according to claim 10, wherein the sugar alcohol content is 1.5 - 10 g/serving.

13. The composition according to claim 1, wherein the mass of the kiwi powder is 0.001 - 5 g per serving.

14. The composition according to any one of claims 1 to 13, which is processed into one or more of the following: tablets, capsules, powders, oral liquids, jellies and gums.

15. The composition according to any one of claims 1 to 13, used to improve constipation, increase defecation frequency in persons with constipation, soften stool without loose stools and reduce the incidence of loose stools during defecation.