NL2033005B1 - Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite - Google Patents
Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite Download PDFInfo
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- NL2033005B1 NL2033005B1 NL2033005A NL2033005A NL2033005B1 NL 2033005 B1 NL2033005 B1 NL 2033005B1 NL 2033005 A NL2033005 A NL 2033005A NL 2033005 A NL2033005 A NL 2033005A NL 2033005 B1 NL2033005 B1 NL 2033005B1
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- 238000000034 method Methods 0.000 title claims abstract description 44
- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 241000238876 Acari Species 0.000 claims abstract description 130
- 235000013601 eggs Nutrition 0.000 claims abstract description 71
- 241000406583 Parasitidae Species 0.000 claims abstract description 36
- 239000000758 substrate Substances 0.000 claims abstract description 34
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 21
- 239000010455 vermiculite Substances 0.000 claims abstract description 21
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 21
- 241000238424 Crustacea Species 0.000 claims abstract description 19
- 241000132121 Acaridae Species 0.000 claims abstract description 13
- 241000260534 Carpoglyphidae Species 0.000 claims abstract description 13
- 241000260747 Suidasiidae Species 0.000 claims abstract description 12
- 238000009395 breeding Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
- 239000011368 organic material Substances 0.000 claims abstract description 10
- 241001466457 Parasitus Species 0.000 claims description 34
- 241000607479 Yersinia pestis Species 0.000 claims description 15
- 206010035148 Plague Diseases 0.000 claims description 14
- 241000261545 Suidasia medanensis Species 0.000 claims description 11
- 241000260546 Carpoglyphus lactis Species 0.000 claims description 10
- 241000611866 Tyrophagus putrescentiae Species 0.000 claims description 9
- 241000238582 Artemia Species 0.000 claims description 8
- 241000630736 Ephestia Species 0.000 claims description 8
- 239000003415 peat Substances 0.000 claims description 8
- 230000009286 beneficial effect Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 241001515858 Artemiidae Species 0.000 claims description 5
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 4
- 244000060011 Cocos nucifera Species 0.000 claims description 4
- 239000002361 compost Substances 0.000 claims description 4
- 238000004382 potting Methods 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 241000260547 Carpoglyphus Species 0.000 claims description 3
- 241000261542 Suidasia Species 0.000 claims description 3
- 241000132125 Tyrophagus Species 0.000 claims description 3
- 206010061217 Infestation Diseases 0.000 claims 1
- 201000002266 mite infestation Diseases 0.000 claims 1
- 239000005416 organic matter Substances 0.000 claims 1
- 210000003608 fece Anatomy 0.000 description 14
- 241000255925 Diptera Species 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 230000009545 invasion Effects 0.000 description 9
- 241001481695 Dermanyssus gallinae Species 0.000 description 8
- 230000000384 rearing effect Effects 0.000 description 7
- 244000062645 predators Species 0.000 description 6
- 241000894243 Sericata Species 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 4
- 241000257162 Lucilia <blowfly> Species 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241001414989 Thysanoptera Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 244000045947 parasite Species 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241000239290 Araneae Species 0.000 description 2
- 241001124169 Calliphora vomitoria Species 0.000 description 2
- 241000233378 Ephydridae Species 0.000 description 2
- 241000238728 Hydrotaea aenescens Species 0.000 description 2
- 241000257159 Musca domestica Species 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 241001494115 Stomoxys calcitrans Species 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 241000238708 Astigmata Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241001177891 Cheyletidae Species 0.000 description 1
- 241000542171 Cunaxidae Species 0.000 description 1
- 241000122098 Ephestia kuehniella Species 0.000 description 1
- 241000331960 Erythraeidae Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000232956 Laelapidae Species 0.000 description 1
- 241000406602 Macrochelidae Species 0.000 description 1
- 241000273338 Macronyssidae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000197722 Sphaeroceridae Species 0.000 description 1
- 241001468463 Tydeidae Species 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- 239000008240 homogeneous mixture Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 244000062804 prey Species 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/30—Rearing or breeding invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/02—Acaricides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Insects & Arthropods (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for cultivating a mite, a mite composition comprising said mite, a system for releasing said mite, and use of said mite. The method for cultivating a mite comprises feeding a breeding population of individuals of the mite of the family Parasitidae in a substrate, comprising organic material and vermiculite, with individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths.
Description
METHOD FOR CULTIVATING A MITE, COMPOSITION COMPRISING SAID MITE,
SYSTEM FOR RELEASING SAID MITE, AND USE OF SAID MITE
The present invention relates to a method for cultivating a mite, a mite composition comprising said mite, a system for releasing said mite, and use of said mite.
Conventional methods for cultivating a mite and the commercial interest of said mites have increased over the years. For example, biological crop protection in agriculture is gaining momentum, wherein the use of predatory mites for said protection is increased. Currently
Phytoseiid predatory mites are employed to prevent and/or reduce and/or treat undesired invasions of for example asphytophagous mites, thrips, and whiteflies. In addition, other (predatory) mite species selected from Mesostigmatid and Prostigmatid predatory species, such as from the family of the Cheyletidae, Cunaxidae, Macrochelidae, Erythraeidae, Laelapidae, Parasitidae, or Tydeidae, receive interest in biological crop protection.
A driving force behind the increasing interest of predatory mites is their efficacy to control harmful crop pests and the availability of mass rearing systems to produce them on a commercially relevant scale for an acceptable price. Therefore, the use of predatory mites as an economic alternative to chemical pesticides is enabled. Conventional methods may for example include raring the predatory mites on life prey in a culture maintained on a carrier, or to rear predatory mites on eggs from Ephestia kuehniella coated on popcorn grains.
Furthermore, the conventional methods for rearing predatory mites depend heavily on the availability of suitable prey for the predators and the commercially value of said methods.
Therefore, during the last years, there have been invested in different rearing prey (also referred to as rearing hosts) for predatory mites. Particularly, mites from the family of the Astigmata have been identified as such suitable rearing prey. In view of their role in rearing of predatory mites, the commercial relevance of rearing preys is increasing.
These problems prevent an efficient and effective method for cultivating a mite. This problem is even bigger for large scale cultivating of desired mites. Therefore, in view of the above there is a continuing need to improve method for cultivating mites of predatory mites.
The present invention aims at obviating or at least reducing the aforementioned problems and to enable efficient and effective cultivation of mites.
This objective is achieved with the method for cultivating a mite, comprising feeding a breeding population of individuals of the mite of the family Parasitidae in a substrate, comprising organic material and vermiculite, with individuals of further mites of the family Carpoglyphidae,
Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths.
It is noted that feeding includes adding individuals of the further mites of the family
Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths to the breeding population.
An advantage of the method according to the invention is that efficient and effective cultivation of a mite of the family Parasitidae is achieved.
Furthermore, it was found that a stable population of the family Parasitidae was achieved using a substrate coprising organic material and vermiculite, wherein said mites are fed with individuals of further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths. It is noted that feeding the mites of the family Parasitidae with the further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae includes feeding the mites of the family Parasitidae with one or more individual of the further mites of the family
Carpoglyphidae, Acaridae, Suidasiidae. Thus, feeding with individuals of further mites selected from the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof.
Feeding the mites of the family Parasitidae with individuals of further mites of the family
Carpoglyphidae, Acaridae, and/or Suidastidae, and eggs of crustacean and/or eggs of moths provides sufficient nutrients for the mites of the family Parasitidae to grow and/or reproduce.
It is noted that all stadia of the individuals of the further mites may be used for the cultivation of the mites of the family Parasitidae. For example, but not limited to, the different stadia may be eggs of the further mites, lava’s of the further mites, nymphs of the further mites, adults of the further mites. In addition, said mites in their different stadia of evolution may be fed to the mite of the family Parasitidae alive or dead.
In a preferred embodiment according to the invention, the method may start with a step of providing a substrate comprising organic material and vermiculite. Said step may be followed by a step of adding a breeding population of individuals of the mite of the family Parasitidae to the substrate, and a step of feeding the mite with individuals of further mites of the family
Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths.
In a further preferred embodiment according to the invention, the mites of the family
Parasitidae are fed with individuals of the family Carpoglyphidae, Acaridae, and Suidasiidae, and eggs of crustacean and/or eggs of moths.
It was found that efficient and effective cultivation of mites of the family Parasitidae was achieved.
In a presently preferred embodiment according to the invention, the mite may be of the genus Parasitus.
It was found that the method according to the invention is in particular effective and efficient for cultivation of the mite of the genus Parasttus. Furthermore. mites of the genus Parasitus may efficiently be used as a predator mite. In addition, after curing an invasion of undesired mites and/or flies with the predator mite of the genus Parasitus, the mites of Parasitus only live for about another two weeks as the circle of life is about two weeks for mites of the genus Parasilus.
In a further presently preferred embodiment according to the invention, the mite may be
Parasitus consanguineous.
An advantage of the mite being Parasitus consanguineous is that cultivation of said mites is cost effective. Therefore, the method according to the invention is an improvement and/or alternative for conventional methods.
A further advantage of the mite being Parasitus consanguineous is that cultivation of said mites does not require intensive labour. Therefore, intensive training to use and/or cultivate said mites is reduced and/or unnecessary.
In a further presently preferred embodiment according to the invention, the further mites are of the genus Carpoglyphus, Tyrophagus, Suidasia, or a combination thereof. Preferably, the further mites are Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, or combinations thereof.
It was found that individuals of further mites selected from the group of Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, or combinations thereof, are efficient and effective nutrient sources. As a result, the mites of the family Parasitidae are enabled to cultivate efficiently and effectively.
In a further presently preferred embodiment according to the invention, the organic material may be one or more selected from the group of peat, coconut substrate, potting soil, compost.
Providing organic material wherein the organic material is one or more selected from the group of peat, coconut substrate, potting soil, compost enables to provide the mites of the family
Parasitidae with a sufficient amount of nutrients such as minerals and the like.
In a preferred embodiment, the peat comprises black peat.
In a further presently preferred embodiment according to the invention, the diameter of the eggs may be in the range of 0.1 mm to 3 mm, preferably in the range of 0.5 mm to 2 mm, more preferably in the range of 0.5 mm to 1.5 mm.
It was found that eggs having a diameter which exceeds 3 mm are not consumed by the mite of the family Parasitidae. Therefore, to efficiently and effectively feed the mite of the family
Parasitidae, the diameter of the eggs may be in the range of 0.1 mm to 3 mm, preferably in the range of 0.5 mm to 2 mm, more preferably in the range of 0.5 mm to 1.5 mm.
In a preferred embodiment, the diameter is about 1 mm.
In a further presently preferred embodiment according to the invention, the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia and/or the eggs of moths are eggs of the genus Ephestia.
The eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus
Artemia and/or the eggs of moths are eggs of the genus Ephestia enable to provide the mite of the family Parasitidae with the desired nutrients.
In a further presently preferred embodiment according to the invention, the humidity may be in the range of 40% to 80%, preferably in the range of 50% to 70%, more preferably in the range of 55% to 65% and/or the temperature may be in the range of 20 °C to 30 °C, preferably in the range of 23 °C to 28 °C, more preferably in the range of 25 °C to 27 °C.
It is noted that the method according to the invention is performed in the abovementioned humidity and/or temperature range.
It was found that providing a humidity and/or temperature in the above mentioned range enable an efficient and effective cultivation of the mite of the family Parasitidae.
In a preferred embodiment, the method according to the invention may comprise measuring and/or controlling of the humidity and/or temperature. Said measuring and/or controlling may be combined, wherein the controlling may occur after a measurement. Furthermore, said measuring and/or controlling may be performed manually, in a semi-autonomous mode, or an autonomous mode.
In a further presently preferred embodiment according to the invention, the method further comprises isolating the mite in vermiculite.
Isolating the mite in vermiculite enables to efficiently and effectively provide the mite of the family Parasitidae to a consumer. Furthermore, this enables to efficiently and effectively use the mite of the family Parasitidae to prevent and/or treat and/or cure an invasion of undesired mites and/or organisms, such as flies, in, for example, agriculture.
Isolating the mite in vermiculite may include the steps of: — providing a net on top of the substrate with the mites of the family Parasitidae; — providing vermiculite on top of said net and providing eggs of crustacean and/or eggs of moths on top of said vermiculite; and — removing the vermiculite which was placed on top of the net.
It was found that said steps enables the mites of the family Parasitidae to migrate to the vermiculite as the eggs attracts said mites. The vermiculite including the mites of the family
Parasitidae may be easily removed from the net and provided to a user and/or used at a place an invasion of undesired mites and/or organisms, such as flies, have to be treated and/or cured.
The invention also relates to a mite composition comprising the mite of the family
Parasitidae and vermiculite.
The mite composition provides the same effects and advantages as those described for the method for cultivating a mite according to the invention.
In a preferred embodiment according to the invention, the mite may be of the genus
Parasitus. Preferably, the mite may be Parasitus consanguineous.
It was found that the mite composition according to the invention is in particular effective and efficient for preventing and/or treating and/or curing an invasion of undesired mites and/or 5 organisms, such as flies. For example, it was found that the mite composition according to the invention efficiently and effectively prevents and/or treats and/or cures an invasion of Lucillia sericata, Calliphora vomitoria, Musca domestica, Stomoxys calcitrans, thrips, spider mite, shore flies (scutella), and root aphids.
Thus, said mites of the genus Parasitus, preferably Parasitus consanguineous, may efficiently be used as a predator mite. In addition, after curing an invasion of undesired mites and/or flies with the predator mite of the genus Parasifus, the mites of Parasitus only live for about another two weeks as the circle of life is about two weeks for mites of the genus Parasirus.
An advantage of the mite being Parasitus consanguineous is that cultivation of said mites is cost effective. Therefore, the mite composition according to the invention is an improvement and/or alternative for conventional mite compositions.
A further advantage of the mite being Parasitus consanguineous is that cultivation of said mites does not require intensive labour. Therefore, intensive training to use and/or cultivate said mites is reduced and/or unnecessary.
In a presently preferred embodiment, the mite composition further comprises a food substrate, wherein the food substrate may be one or more selected from the group of mites of
Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, eggs of crustacean, eggs of moths.
In a preferred embodiment according to the invention, the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia and/or the eggs of moths are eggs of the genus Ephestia.
The invention also relates to a system for releasing beneficial mites, comprising: — a housing suitable for a substrate and provided with an opening; and — mites which are arranged in the housing and which can come out of the opening in order to prevent, reduce, and/or treat a tly plague and/or a mite plague.
The system for releasing beneficial mites provides the same effects and advantages as those described for the method for cultivating a mite according to the invention, and mite composition according to the invention.
By providing a system according to the invention, preferably in the form of a cartridge. the housing of which is suitable for storing a substrate, the mites can deposit their eggs and develop therein. In addition, the housing is provided with an opening. It is possible with the system according to the invention, wherein an opening is provided in the housing, to introduce mites into for instance a house or coop or dung lump. The mites introduced into the house, green house, coop, dung lump in this way will leave the system according to the invention through the opening and spread around the desired area such as the house, green house, coop, dung lump. In the house, green house,coop, dung lump they will keep themselves alive by feeding off the parasites occurring in the animals accommodated in the house and/or green house and/or dung lump and/or coop. Such parasites are, among others, chicken mites or red mites, as well as bird mites. Furthermore, other parasites (undesired mites and/or organisms) such as Lucillia sericata, Calliphora vomitoria,
Musca domestica, Stomoxys calcitrans, thrips, spider mite, shore flies (scutella), and root aphids
The invention also relates to an use of the mite of the family Parasitidae to prevent, reduce, and/or treat a fly plague and/or a mite plague.
The use of the mite of the family Parasitidae to prevent, reduce, and/or treat a fly plague and/or a mite plague to prevent, reduce, and/or treat a fly plague and/or a mite plague provides the same effects and advantages as those described for the method for cultivating a mite according to the invention, mite composition according to the invention, and system for releasing beneficial mites according to the invention.
In a presently preferred embodiment according to the invention, the mite may be provided in an amount in the range of 1000 mites to 3000 mites per square metre, preferably in the range of 1500 mites to 2500 mites per square metre. Preferably, the mites may be provided in multiple amounts, wherein the amounts are provided at least once a week.
Further advantages, features and details of the invention are elucidated on the basis of preferred embodiments thereof, wherein reference is made to the accompanying experiments.
The size of the population of mites of the family Parasitidae (the amount of mites) may be determined using the following method. The substrate comprising the mite of the family
Parasitidae is mixed, such that a homogeneous mixture of substrate and mites is achieved. 0.25 millilitre is taken from the substrate using a calibrated spoon. The mites in the content removed with said spoon is than counted using a microscope. All living mites are counted.
These steps are performed eight times, such that in total 2 millilitre of the substrate comprising said mites is analysed.
The total amount of counted mites is multiplied by 500 to provide the amount of mites per
Litre of substrate.
In an experiment, Parasitus consanguineous were cultivated using a substrate of moist black peat mixed with vermiculite. The ratio between the dry weight of black peak and the dry weight of vermiculite was 1: 5.
The initial colony of about 1000 mites were extensively fed with individuals of further mites of the family Carpoglyphidae. Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths.
In one of the experiments, the Parasitus consanguineous were fed with Carpoglyphus lactis,
Tvrophagus putrescentiae, and Suidasia medanensis, and eggs of the genus Artemia.
In an alternative experiment, the Parasitus consanguineous were fed with Carpoglyphus lactis, Tyrophagus putrescentiae, and Suidasia medanensis, and eggs of the genus Ephestia.
In a further alternative experiment, the Parasitus consanguineous were fed with
Carpoglyphus lactis, Tyrophagus putrescentiae, and Suidasia medanensis, and eggs of the genus
Artemia, and eggs of the genus Ephestia.
It was found that in particular the combination of Carpoglyphus lactis, Tyrophagus putrescentiae, and Suidasia medanensis, and eggs of the genus Artemia, and eggs of the genus
Ephestia enables an efficient and effective cultivation of the Parasitus consanguineous.
Furthermore, the substrate comprising the initial colony has been kept in an environment with a humidity of about 60%, and a temperature of about 26 °C. In addition, a day/night rhythm comprising 16 hours of light, preferably 16 hours of consecutive light, was provided to the substrate comprising the initial colony for 10 days to 14 days.
It was found that at about 12 days the substrate comprises between 45000 and 55000 mites of Parasitus consanguineous per Litre.
In a further experiment about 1 million fly eggs of the fly Hydrotaea aenescens were provided to an enclosed breeding tank. Furthermore, about 50000 mites of Parasitus consanguineous in vermucilite were provided to the breeding tank. The breeding tank was sealed such that flies and/or mites could not escape, bat that the humidity and temperature could be controlled such that optimal developing conditions for the fly Hvdrotaea aenescens were achieved.
After four days the breeding tank was opened and no eggs of the fly Hydrotaea aenescens were found. Therefore, it can be concluded that the mites of Parasitus consanguineous consumed all the eggs of the fly Hvdrotaea aenescens.
In a further experiment, two petri dishes were provided with about 20 mites of Parasitus consanguineous. Furthermore, two maggots of the fly Lucilfia sericata were provided to each dish.
The dishes were sealed such that flies and/or mites could not escape, bat that the humidity and temperature could be controlled such that optimal developing conditions for the maggots of the fly
Lucillia sericata were achieved. After four days the maggots transformed into pupas. Said pupas did not develop into flies. It is proposed that the maggots of the fly Lucillia sericata may not fully develop due to the presence of said mites. When said dishes were opened after six days, the mites were still alive.
In a further experiment, about 100000 mites of Parasitus consanguineous, divided over about 8 Litre of vermiculite, was provided to a dung lump of about 20 m®.
A first dung lump was formed by the feces of chicken and a second a dung lump was formed by the feces of goats. Both dung lump were invaded by flies, such as flies from the family
Sphaeroceridae. After six weeks both dung lumps were cleared from said flies. A sample of both dung lumps showed that the Parasitus consanguineous was still present in both dung lumps.
Therefore, it is concluded that the mite composition according to the invention provides an efficient and effective cure of an invasion of flies.
In a further experiment, a first petri dish is provided with 30 millilitre substrate of peat and vermiculite comprising about 50000 Parasitus consanguineous per Litre substrate. The second petri disch was provided with the same substrate without any predator mite.
Both petri dishes were provided with about 20000 mites of Dermanyssus gallinae. After two weeks all mites of Dermanyssus gallinae in the first petri dish were killed. In the second petri dish the mites of Dermanyssus gallinae were still present.
It is concluded that the mites of Parasitus consanguineous interfere with the evolution of mites of Dermanyssus gallinae. Therefore, the mites of Parasitus consanguineous are enabled to clear an invasion of mites of Dermanyssus gallinae.
In a further experiment, a chicken coop (1 metre by 2 metres) invaded by the mite
Dermanyssus gallinae was cleared by the mite Parasitus consanguineous. The mite Parasitus consanguineous was provided to the chicken coop using four systems for releasing beneficial mites according to the invention. All systems comprises about 1000 mites of Parasitus consanguineous.
After four weeks all no mites of Dermanyssus gallinae were found in the chicken coop.
The present invention is by no means limited to the above described preferred embodiments and/or experiments thereof. The rights sought are defined by the following claims within the scope of which many modifications can be envisaged.
CLAUSES
1. Method for cultivating a mite, comprising feeding a breeding population of individuals of the mite of the family Parasitidae in a substrate, comprising organic material and vermiculite, with individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths. 2. Method according to clause 1, wherein the mite is of the genus Parasitus. 3. Method according to clause 2, wherein the mite is Parasiius consanguineous. 4, Method according to any one of the preceding clauses, wherein the further mites are of the genus Carpoglyphus, Tyrophagus, Suidasia, or a combination thereof. 5. Method according to clause 4, wherein the further mites are Carpoglyphus lactis,
Tvrophagus putrescentiae, Suidasia medanensis, or combinations thereof. 6. Method according to any one of the preceding clauses, wherein the organic material is one or more selected from the group of peat, coconut substrate, potting soil, compost. 7. Method according to any one of the preceding clauses, wherein the diameter of the eggs is in the range of 0.1 mm to 3 mm, preferably in the range of 0.5 mm to 2 mm, more preferably in the range of 0.5 mm to 1.5 mm. 8. Method according to any one of the preceding clauses, wherein the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia. 9. Method according to any one of the preceding clauses, wherein the eggs of moths are eggs of the genus Ephestia. 10. Method according to any one of the preceding clauses, wherein the humidity is in the range of 40% to 80%, preferably in the range of 50% to 70%, more preferably in the range of 55% to 65%.
11. Method according to any one of the preceding clauses, wherein the temperature is in the range of 20 °C to 30 °C, preferably in the range of 23 °C to 28 °C, more preferably in the range of 25 °C to 27 °C.
12. Method according to any one of the preceding clauses, further comprising isolating the mite in vermiculite.
13. Mite composition comprising the mite of the family Parasitidae and vermiculite.
14. Mite composition according to clause 13, further comprising a food substrate, wherein the food substrate is one or more selected from the group of mites of Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, eggs of crustacean, eggs of moths.
15. System for releasing beneficial mites, comprising:
— a housing suitable for a substrate and provided with an opening; and
~ mites which are arranged in the housing and which can come out of the opening in order to prevent, reduce, and/or treat a fly plague and/or a mite plague.
16. Use of the mite of the family Parasitidae to prevent, reduce, and/or treat a fly plague and/or a mite plague.
17. Use according to clause 16, wherein the mite is provided in an amount in the range of 1000 mites to 3000 mites per square metre, preferably in the range of 1500 mites to 2500 mites per square metre.
18. Use according to clause 16 or 17, wherein the mites are provided in multiple amounts, wherein the amounts are provided at least once a week.
Claims (18)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2033005A NL2033005B1 (en) | 2022-09-12 | 2022-09-12 | Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite |
| EP23772976.9A EP4586814A1 (en) | 2022-09-12 | 2023-09-12 | Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite |
| PCT/NL2023/050465 WO2024058656A1 (en) | 2022-09-12 | 2023-09-12 | Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2033005A NL2033005B1 (en) | 2022-09-12 | 2022-09-12 | Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2033005B1 true NL2033005B1 (en) | 2024-03-22 |
Family
ID=83506145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2033005A NL2033005B1 (en) | 2022-09-12 | 2022-09-12 | Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4586814A1 (en) |
| NL (1) | NL2033005B1 (en) |
| WO (1) | WO2024058656A1 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2612551A1 (en) * | 2012-01-04 | 2013-07-10 | Koppert B.V. | Mite composition comprising a predatory mite and immobilized prey contacted with a fungus reducing agent and methods and uses related to the use of said composition |
| CN103563855A (en) * | 2013-05-20 | 2014-02-12 | 中国农业科学院植物保护研究所 | New method for feeding prey mites and predatory mites |
| WO2019017776A1 (en) * | 2017-07-16 | 2019-01-24 | Koppert B.V. | System for releasing beneficial mites and uses thereof |
| WO2022185303A1 (en) * | 2021-03-01 | 2022-09-09 | Bio-Bee Sde Eliyahu Ltd | Food source for biological control agents and methods thereof |
-
2022
- 2022-09-12 NL NL2033005A patent/NL2033005B1/en active
-
2023
- 2023-09-12 WO PCT/NL2023/050465 patent/WO2024058656A1/en not_active Ceased
- 2023-09-12 EP EP23772976.9A patent/EP4586814A1/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2612551A1 (en) * | 2012-01-04 | 2013-07-10 | Koppert B.V. | Mite composition comprising a predatory mite and immobilized prey contacted with a fungus reducing agent and methods and uses related to the use of said composition |
| CN103563855A (en) * | 2013-05-20 | 2014-02-12 | 中国农业科学院植物保护研究所 | New method for feeding prey mites and predatory mites |
| WO2019017776A1 (en) * | 2017-07-16 | 2019-01-24 | Koppert B.V. | System for releasing beneficial mites and uses thereof |
| WO2022185303A1 (en) * | 2021-03-01 | 2022-09-09 | Bio-Bee Sde Eliyahu Ltd | Food source for biological control agents and methods thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2024058656A1 (en) | 2024-03-21 |
| EP4586814A1 (en) | 2025-07-23 |
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