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NL2031841B1 - Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation - Google Patents

Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation Download PDF

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Publication number
NL2031841B1
NL2031841B1 NL2031841A NL2031841A NL2031841B1 NL 2031841 B1 NL2031841 B1 NL 2031841B1 NL 2031841 A NL2031841 A NL 2031841A NL 2031841 A NL2031841 A NL 2031841A NL 2031841 B1 NL2031841 B1 NL 2031841B1
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NL
Netherlands
Prior art keywords
ghpmei39
gene
pectin methylesterase
inhibitor gene
methylesterase inhibitor
Prior art date
Application number
NL2031841A
Other languages
English (en)
Inventor
Wei Zhenzhen
Li Fuguang
Mao Xiaonan
Wang Zhi
Li Yonghui
Original Assignee
Institute Of Cotton Res Of Caas
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Publication date
Application filed by Institute Of Cotton Res Of Caas filed Critical Institute Of Cotton Res Of Caas
Priority to NL2031841A priority Critical patent/NL2031841B1/en
Application granted granted Critical
Publication of NL2031841B1 publication Critical patent/NL2031841B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011—Pectinesterase (3.1.1.11)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Claims (6)

Conclusies
1. Een pectine-methylesteraseremmer-gen GhPMEI39, waarbij een nucleinezuursequentie van het gen wordt uiteengezet in SEQ ID NO:1.
2. Een methode voor het detecteren van katoenvariéteiten, bestaande uit de volgende stappen: het detecteren of een te testen monster tot overexpressie gebracht pectine-methylesteraseremmer-gen GhPMEI39 of een product geproduceerd door tot overexpressie gebracht pectine-methylesteraseremmer- gen GhPMEI39 bevat; en, beoordelen dat het te testen monster een hoge katoenopbrengst heeft als het wordt gedetecteerd, waarbij een nucleinezuursequentie van het pectinemethylesteraseremmergen GhPMEI39 wordt uiteengezet in SEQ ID NO:1.
3. De methode voor het detecteren van katoenvariëteiten, volgens conclusie 2, waarbij voor het detecteren van het te testen monster primerparen of probes of chips worden gebruikt die zijn ontworpen door de elementen van het pectine- methylesterase-repressorgen GhPMEI39 tot overexpressie te brengen.
4. De methode voor het detecteren van katoenvariëteiten, volgens conclusie 3, waarbij nucleinezuursequenties van de primerparen worden uiteengezet in SEQ ID NO:2 en SEQ ID NO:3.
5. De methode voor het detecteren van katoenvariëteiten, volgens een van de conclusies 2-4, waarbij het te testen monster een materiaal omvat dat geschikt is voor seksuele reproductie, ongeslachtelijke reproductie of weefselkweek van regenereerbare cellen.
6. De methode voor het detecteren van katoenvariëteiten, volgens conclusie 5, waarbij het te detecteren monster is gekozen uit een van de volgende materialen: zaden, bladeren, wortels, stengels, kiemwortels en embryo's.
NL2031841A 2022-05-12 2022-05-12 Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation NL2031841B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL2031841A NL2031841B1 (en) 2022-05-12 2022-05-12 Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL2031841A NL2031841B1 (en) 2022-05-12 2022-05-12 Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation

Publications (1)

Publication Number Publication Date
NL2031841B1 true NL2031841B1 (en) 2023-11-20

Family

ID=88838478

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2031841A NL2031841B1 (en) 2022-05-12 2022-05-12 Application of Pectin Methylesterase Inhibitor Gene GhPMEI39 and its Encoded Protein in Plant Inflorescence Regulation

Country Status (1)

Country Link
NL (1) NL2031841B1 (nl)

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