NL2018134B1 - Forming images of a sample using scanning microscopy - Google Patents
Forming images of a sample using scanning microscopy Download PDFInfo
- Publication number
- NL2018134B1 NL2018134B1 NL2018134A NL2018134A NL2018134B1 NL 2018134 B1 NL2018134 B1 NL 2018134B1 NL 2018134 A NL2018134 A NL 2018134A NL 2018134 A NL2018134 A NL 2018134A NL 2018134 B1 NL2018134 B1 NL 2018134B1
- Authority
- NL
- Netherlands
- Prior art keywords
- emission light
- optical
- emission
- mirror
- light signal
- Prior art date
Links
- 238000004621 scanning probe microscopy Methods 0.000 title abstract description 38
- 230000003287 optical effect Effects 0.000 claims abstract description 228
- 238000005286 illumination Methods 0.000 claims abstract description 148
- 238000003384 imaging method Methods 0.000 claims abstract description 91
- 230000005284 excitation Effects 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims abstract description 54
- 238000000386 microscopy Methods 0.000 claims description 11
- 238000004590 computer program Methods 0.000 claims description 8
- 230000010363 phase shift Effects 0.000 claims description 8
- 230000004044 response Effects 0.000 claims description 3
- 238000005424 photoluminescence Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 description 18
- 238000005259 measurement Methods 0.000 description 10
- 230000010354 integration Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 230000010287 polarization Effects 0.000 description 5
- 230000003068 static effect Effects 0.000 description 5
- 238000004020 luminiscence type Methods 0.000 description 4
- 238000004624 confocal microscopy Methods 0.000 description 3
- 239000012620 biological material Substances 0.000 description 2
- 238000001444 catalytic combustion detection Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- 230000036962 time dependent Effects 0.000 description 2
- 238000001429 visible spectrum Methods 0.000 description 2
- 239000011149 active material Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002292 fluorescence lifetime imaging microscopy Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010869 super-resolution microscopy Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2018134A NL2018134B1 (en) | 2017-01-05 | 2017-01-05 | Forming images of a sample using scanning microscopy |
| PCT/EP2018/050280 WO2018127566A1 (fr) | 2017-01-05 | 2018-01-05 | Formation d'images d'un échantillon à l'aide d'une microscopie à balayage |
| EP18700268.8A EP3566089A1 (fr) | 2017-01-05 | 2018-01-05 | Formation d'images d'un échantillon à l'aide d'une microscopie à balayage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2018134A NL2018134B1 (en) | 2017-01-05 | 2017-01-05 | Forming images of a sample using scanning microscopy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2018134B1 true NL2018134B1 (en) | 2018-07-25 |
Family
ID=58455598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2018134A NL2018134B1 (en) | 2017-01-05 | 2017-01-05 | Forming images of a sample using scanning microscopy |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP3566089A1 (fr) |
| NL (1) | NL2018134B1 (fr) |
| WO (1) | WO2018127566A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109188667B (zh) * | 2018-08-10 | 2021-03-12 | 国家纳米科学中心 | 多光束阵列多光子重扫描显微成像装置 |
| NL2028507B1 (en) | 2021-06-22 | 2022-12-29 | Confocal Nl B V | Rescan optical system, microscope and method |
| CA3225833A1 (fr) * | 2021-07-15 | 2023-01-19 | Sheng Xiao | Multiplicateur de balayage optique et ses utilisations |
| EP4550020A1 (fr) | 2023-11-03 | 2025-05-07 | Confocal.nl B.V. | Système optique de rébalayage |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015164843A1 (fr) * | 2014-04-24 | 2015-10-29 | Vutara, Inc. | Miroir de balayage galvanique pour microscopie de super-résolution |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060238756A1 (en) * | 2005-02-10 | 2006-10-26 | Bearman Gregory H | Fast spectral confocal imager |
| DE102005042672B4 (de) * | 2005-09-08 | 2009-10-01 | Leica Microsystems Cms Gmbh | Photosensor-Chip, Laser-Mikroskop mit Photosensor-Chip und Verfahren zum Auslesen eines Photosensor-Chips |
| DE102012023024B4 (de) * | 2012-11-07 | 2023-05-04 | Carl Zeiss Microscopy Gmbh | Lichtmikroskop und Mikroskopieverfahren |
| EP3198324B1 (fr) * | 2014-09-24 | 2020-12-23 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Augmentation de la résolution pour systèmes de microscopie par excitation à balayage linéaire et procédés |
-
2017
- 2017-01-05 NL NL2018134A patent/NL2018134B1/en not_active IP Right Cessation
-
2018
- 2018-01-05 WO PCT/EP2018/050280 patent/WO2018127566A1/fr not_active Ceased
- 2018-01-05 EP EP18700268.8A patent/EP3566089A1/fr not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015164843A1 (fr) * | 2014-04-24 | 2015-10-29 | Vutara, Inc. | Miroir de balayage galvanique pour microscopie de super-résolution |
Non-Patent Citations (2)
| Title |
|---|
| GIULIA M.R. DE LUCA ET AL: "Re-scan confocal microscopy: scanning twice for better resolution", BIOMEDICAL OPTICS EXPRESS, vol. 4, no. 11, 25 October 2013 (2013-10-25), pages 2644, XP055141694, ISSN: 2156-7085, DOI: 10.1364/BOE.4.002644 * |
| YUANSHENG SUN ET AL: "Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy", NATURE PROTOCOLS, vol. 6, no. 9, 11 August 2011 (2011-08-11), GB, pages 1324 - 1340, XP055409337, ISSN: 1754-2189, DOI: 10.1038/nprot.2011.364 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018127566A1 (fr) | 2018-07-12 |
| EP3566089A1 (fr) | 2019-11-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PD | Change of ownership |
Owner name: CONFOCAL.NL B.V.; NL Free format text: DETAILS ASSIGNMENT: CHANGE OF OWNER(S), ASSIGNMENT; FORMER OWNER NAME: UNIVERSITEIT VAN AMSTERDAM Effective date: 20190222 |
|
| MM | Lapsed because of non-payment of the annual fee |
Effective date: 20230201 |