MY198384A - Expression Vector for Microalga Cell Transformation - Google Patents
Expression Vector for Microalga Cell TransformationInfo
- Publication number
- MY198384A MY198384A MYPI2020000621A MYPI2020000621A MY198384A MY 198384 A MY198384 A MY 198384A MY PI2020000621 A MYPI2020000621 A MY PI2020000621A MY PI2020000621 A MYPI2020000621 A MY PI2020000621A MY 198384 A MY198384 A MY 198384A
- Authority
- MY
- Malaysia
- Prior art keywords
- gene
- expression
- expression vector
- ompk
- vcp
- Prior art date
Links
- 239000013604 expression vector Substances 0.000 title abstract 4
- 230000010307 cell transformation Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 241000509521 Nannochloropsis sp. Species 0.000 abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 230000000890 antigenic effect Effects 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 108090000913 Nitrate Reductases Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000607618 Vibrio harveyi Species 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses an expression vector for transforming algal cells, which comprises an endogenous promoter, an antigenic protein fragment and an antibiotic resistance gene. The expression vector is configured to introduce a gene into Nannochloropsis sp. such that the gene is expressed and functional. This transforms the alga Nannochloropsis sp. with high efficiency, which makes it useful for various applications including, in the aquaculture industry. In an embedment, the expression vectors for Nannochloropsis sp. according to the present invention are comprised of two sets of nucleotide sequences and referred to as VCP_OMPK_ZA1 and VCP_OMPK_ZA2. Both the vectors contain a fragment of nitrate reductase gene, which is configured to replace a partial fragment of the gene with an expression system expressing antigenic protein called OmpK originated from Vibrio harveyi. Expression of both the vectors are driven by endogenous violaxanthin-chlorophyll protein (VCP) promoter and a terminator, respectively. Figure 5
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2020000621A MY198384A (en) | 2020-02-04 | 2020-02-04 | Expression Vector for Microalga Cell Transformation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2020000621A MY198384A (en) | 2020-02-04 | 2020-02-04 | Expression Vector for Microalga Cell Transformation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY198384A true MY198384A (en) | 2023-08-28 |
Family
ID=90931735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI2020000621A MY198384A (en) | 2020-02-04 | 2020-02-04 | Expression Vector for Microalga Cell Transformation |
Country Status (1)
| Country | Link |
|---|---|
| MY (1) | MY198384A (en) |
-
2020
- 2020-02-04 MY MYPI2020000621A patent/MY198384A/en unknown
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