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MX2024001110A - Adn lineal con resistencia potenciada contra exonucleasas y metodos para la produccion de los mismos. - Google Patents

Adn lineal con resistencia potenciada contra exonucleasas y metodos para la produccion de los mismos.

Info

Publication number
MX2024001110A
MX2024001110A MX2024001110A MX2024001110A MX2024001110A MX 2024001110 A MX2024001110 A MX 2024001110A MX 2024001110 A MX2024001110 A MX 2024001110A MX 2024001110 A MX2024001110 A MX 2024001110A MX 2024001110 A MX2024001110 A MX 2024001110A
Authority
MX
Mexico
Prior art keywords
methods
enhanced resistance
production
resistance against
dna
Prior art date
Application number
MX2024001110A
Other languages
English (en)
Inventor
Ángel Picher
Heikki Lanckriet
Amy Walker
Original Assignee
4Basebio S L U
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 4Basebio S L U filed Critical 4Basebio S L U
Publication of MX2024001110A publication Critical patent/MX2024001110A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para producir un producto de ácido desoxirribonucleico (ADN) lineal con resistencia potenciada para digestión con nucleasa. Los métodos comprenden: (a) poner en contacto una molécula de ADN de doble hebra con una endonucleasa, una ligasa y primera y segunda moléculas adaptadoras para formar un único volumen acuoso contiguo; y (b) incubar el único volumen acuoso contiguo para generar un producto de ADN lineal. También se proporcionan productos de ácido desoxirribonucleico (ADN) lineal con resistencia potenciada para digestión con nucleasa y usos de los mismos.
MX2024001110A 2021-07-30 2022-07-29 Adn lineal con resistencia potenciada contra exonucleasas y metodos para la produccion de los mismos. MX2024001110A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21382725.6A EP4124660A1 (en) 2021-07-30 2021-07-30 Linear dna with enhanced resistance against exonucleases and methods for the production thereof
PCT/EP2022/071413 WO2023006978A1 (en) 2021-07-30 2022-07-29 Linear dna with enhanced resistance against exonucleases and methods for the production thereof

Publications (1)

Publication Number Publication Date
MX2024001110A true MX2024001110A (es) 2024-05-09

Family

ID=77168191

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2024001110A MX2024001110A (es) 2021-07-30 2022-07-29 Adn lineal con resistencia potenciada contra exonucleasas y metodos para la produccion de los mismos.

Country Status (11)

Country Link
US (1) US20240076659A1 (es)
EP (1) EP4124660A1 (es)
JP (1) JP2024528060A (es)
KR (1) KR20240038989A (es)
CN (1) CN117980501A (es)
AU (1) AU2022320939A1 (es)
CA (1) CA3224561A1 (es)
ES (3) ES2947031T6 (es)
GB (3) GB2631830B (es)
MX (1) MX2024001110A (es)
WO (1) WO2023006978A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4553156A1 (en) 2023-11-08 2025-05-14 4basebio UK Ltd Synthetic dna production using cas nuclease
WO2025120370A1 (en) * 2023-12-08 2025-06-12 Camena Bioscience Limited Compositions and methods for solution-phase, phosphoramidite-free synthesis of nucleic acids
EP4596713A1 (en) 2024-02-05 2025-08-06 4basebio UK Ltd Dna with enhanced resistance against exonucleases and methods for the production thereof
EP4628594A1 (en) 2024-04-05 2025-10-08 4basebio UK Ltd Methods of amplifying dna and dna products with inhibitory regions
US20250340934A1 (en) * 2024-05-06 2025-11-06 Gt Molecular, Inc. Selective blocking to detect and amplify low abundant template
WO2025238067A1 (en) 2024-05-15 2025-11-20 Ascend Advanced Therapies Gmbh Novel vector system for aav production

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101414713B1 (ko) * 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
CN114616338A (zh) * 2019-08-22 2022-06-10 新加坡国立大学 生成哑铃形dna载体的方法
EP3845667A1 (en) * 2019-12-30 2021-07-07 Industrial Technology Research Institute Method of amplifying and determining target nucleotide sequence
WO2021152147A1 (en) * 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
WO2021152146A1 (en) * 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Process for the production of closed linear dna
GB202014751D0 (en) * 2020-09-18 2020-11-04 Lightbio Ltd Targeting vector
CA3226457A1 (en) * 2021-07-30 2023-02-02 Amy WALKER Nanoparticles and peptides for the delivery of cargos to muscle cells
WO2023230991A1 (zh) * 2022-06-02 2023-12-07 宜明(苏州)细胞生物科技有限公司 新型闭合线性双链dna的体外制备技术

Also Published As

Publication number Publication date
CA3224561A1 (en) 2023-02-02
GB2621500B (en) 2024-07-03
EP4124660A1 (en) 2023-02-01
GB202508033D0 (en) 2025-07-09
GB2631830A (en) 2025-01-15
US20240076659A1 (en) 2024-03-07
AU2022320939A1 (en) 2024-02-29
ES2996704A2 (es) 2025-02-13
GB202317071D0 (en) 2023-12-20
WO2023006978A1 (en) 2023-02-02
ES2956243A2 (es) 2023-12-15
ES2947031T6 (es) 2023-08-02
JP2024528060A (ja) 2024-07-26
ES2956243B2 (es) 2024-11-26
GB202407102D0 (en) 2024-07-03
GB2700069A (en) 2025-09-10
GB2621500A (en) 2024-02-14
KR20240038989A (ko) 2024-03-26
ES2956243R1 (es) 2024-08-07
ES2996704R1 (es) 2025-07-16
GB2631830B (en) 2025-10-08
CN117980501A (zh) 2024-05-03

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