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MX2023013737A - Secuenciador volumétrico in situ de nueva generación. - Google Patents

Secuenciador volumétrico in situ de nueva generación.

Info

Publication number
MX2023013737A
MX2023013737A MX2023013737A MX2023013737A MX2023013737A MX 2023013737 A MX2023013737 A MX 2023013737A MX 2023013737 A MX2023013737 A MX 2023013737A MX 2023013737 A MX2023013737 A MX 2023013737A MX 2023013737 A MX2023013737 A MX 2023013737A
Authority
MX
Mexico
Prior art keywords
sequencer
situ
volumetric
generation
automated
Prior art date
Application number
MX2023013737A
Other languages
English (en)
Inventor
DEISSEROTH Karl A
Richman Ethan B
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of MX2023013737A publication Critical patent/MX2023013737A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Optics & Photonics (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Particle Accelerators (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Se proporciona un secuenciador para la secuenciación in situ automatizada de muestras volumétricas de tejido. En particular, se proporciona un dispositivo volumétrico automatizado de secuenciación in situ capaz de operar en múltiples muestras en paralelo. También se proporcionan métodos para fabricación y utilización del secuenciador.
MX2023013737A 2021-05-21 2022-05-20 Secuenciador volumétrico in situ de nueva generación. MX2023013737A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163191460P 2021-05-21 2021-05-21
PCT/US2022/030232 WO2022246181A2 (en) 2021-05-21 2022-05-20 Volumetric next-generation in situ sequencer

Publications (1)

Publication Number Publication Date
MX2023013737A true MX2023013737A (es) 2024-02-13

Family

ID=84141873

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023013737A MX2023013737A (es) 2021-05-21 2022-05-20 Secuenciador volumétrico in situ de nueva generación.

Country Status (10)

Country Link
US (1) US20240248038A1 (es)
EP (1) EP4351787A4 (es)
JP (1) JP2024521143A (es)
KR (1) KR20240023042A (es)
CN (1) CN117957060A (es)
AU (1) AU2022276432A1 (es)
CA (1) CA3220594A1 (es)
IL (1) IL308572A (es)
MX (1) MX2023013737A (es)
WO (1) WO2022246181A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112243463B (zh) 2018-04-09 2024-12-13 小利兰·斯坦福大学托管委员会 原位基因测序方法
WO2022245689A2 (en) * 2021-05-19 2022-11-24 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Automated nanoscopy system having integrated artifact minimization modules, including embedded nanometer position tracking based on phasor analysis
WO2025199703A1 (zh) * 2024-03-25 2025-10-02 深圳华大智造科技股份有限公司 基因测序系统、方法、设备及非易失性存储介质

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5597868B2 (ja) * 2006-09-07 2014-10-01 ライカ マイクロシステムズ ツェーエムエス ゲーエムベーハー 液浸対物レンズ、液浸膜を形成する装置及び方法
JP5492207B2 (ja) * 2008-08-27 2014-05-14 ライフ テクノロジーズ コーポレーション 生物学的サンプルの処理装置および処理方法
WO2015081242A2 (en) * 2013-11-27 2015-06-04 Beckman Coulter, Inc. Fluidics system for flow cytometer
US10005080B2 (en) * 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
WO2019016048A1 (en) * 2017-07-17 2019-01-24 Novartis Ag DIP DECK FOR MICROSCOPE, MICROSCOPE, AND METHOD FOR MICROSCOPIC IMAGING SAMPLE
US11041199B2 (en) * 2018-12-20 2021-06-22 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11747605B2 (en) * 2019-04-23 2023-09-05 Molecular Devices, Llc System and method for formation and detection of immersion fluid boluses on a microscope objective

Also Published As

Publication number Publication date
CN117957060A (zh) 2024-04-30
AU2022276432A1 (en) 2023-12-14
KR20240023042A (ko) 2024-02-20
EP4351787A2 (en) 2024-04-17
EP4351787A4 (en) 2025-05-21
CA3220594A1 (en) 2022-11-24
JP2024521143A (ja) 2024-05-28
IL308572A (en) 2024-01-01
WO2022246181A9 (en) 2023-11-02
WO2022246181A3 (en) 2022-12-29
WO2022246181A2 (en) 2022-11-24
US20240248038A1 (en) 2024-07-25

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