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MX2023010649A - Analisis de expresion de variantes codificantes de proteinas en celulas. - Google Patents

Analisis de expresion de variantes codificantes de proteinas en celulas.

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Publication number
MX2023010649A
MX2023010649A MX2023010649A MX2023010649A MX2023010649A MX 2023010649 A MX2023010649 A MX 2023010649A MX 2023010649 A MX2023010649 A MX 2023010649A MX 2023010649 A MX2023010649 A MX 2023010649A MX 2023010649 A MX2023010649 A MX 2023010649A
Authority
MX
Mexico
Prior art keywords
expression
variant
cell
barcode
cdna
Prior art date
Application number
MX2023010649A
Other languages
English (en)
Inventor
Kai-How FARH
Dan Cao
Tong Liu
Shi Min Xiao
Hongxia Xu
Victor Quijano
Mohan Sun
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2023010649A publication Critical patent/MX2023010649A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

En la presente descripción se analiza la expresión de variantes codificantes de proteínas en células. Un método puede incluir reemplazar una región codificante de proteínas de ADN en una célula con un vector donante que incluye una variante de la región codificante de proteínas y un primer código de barras que identifica esa variante. La célula puede generar ARNm que incluye una expresión de la variante y una expresión del primer código de barras. Un segundo código de barras que corresponde a la célula puede acoplarse al ARNm. El ARNm, que tiene el segundo código de barras acoplado a este, puede transcribirse de manera inversa en ADNc complementario. El ADNc puede secuenciarse. El vector donante o ADNc puede secuenciarse usando secuenciación de amplicones. La secuencia del vector donante y la secuencia de ADNc pueden correlacionarse para identificar la variante y la expresión de la célula de la variante.
MX2023010649A 2021-03-09 2022-03-08 Analisis de expresion de variantes codificantes de proteinas en celulas. MX2023010649A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163158492P 2021-03-09 2021-03-09
US202163162775P 2021-03-18 2021-03-18
US202163163381P 2021-03-19 2021-03-19
US202163226424P 2021-07-28 2021-07-28
PCT/US2022/019258 WO2022192191A1 (en) 2021-03-09 2022-03-08 Analyzing expression of protein-coding variants in cells

Publications (1)

Publication Number Publication Date
MX2023010649A true MX2023010649A (es) 2023-09-20

Family

ID=81306963

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023010649A MX2023010649A (es) 2021-03-09 2022-03-08 Analisis de expresion de variantes codificantes de proteinas en celulas.

Country Status (10)

Country Link
US (1) US20240167020A1 (es)
EP (1) EP4305164A1 (es)
JP (1) JP2024509446A (es)
KR (3) KR20240150520A (es)
AU (1) AU2022232600A1 (es)
BR (1) BR112023018157A2 (es)
CA (1) CA3209070A1 (es)
IL (2) IL305151B2 (es)
MX (1) MX2023010649A (es)
WO (1) WO2022192191A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024233881A1 (en) * 2023-05-10 2024-11-14 Illumina, Inc. Machine learning and bioinformatic methods for variant interpretation

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
WO1994024143A1 (en) 1993-04-12 1994-10-27 Northwestern University Method of forming oligonucleotides
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
KR100189229B1 (ko) 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
EP0914462A4 (en) 1996-03-18 2002-05-22 Molecular Biology Resources Amplification of target nucleic acid sequences
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
AU773536B2 (en) 1999-03-19 2004-05-27 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
EA005739B1 (ru) 2000-08-23 2005-06-30 Такара Био Инк. Способ амплификации нуклеиновых кислот
AU2003297557B2 (en) 2002-11-21 2009-02-26 Cellscript, Inc. Methods for using primers that encode one strand of a double-stranded promoter
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
WO2017223538A1 (en) * 2016-06-24 2017-12-28 The Regents Of The University Of Colorado, A Body Corporate Methods for generating barcoded combinatorial libraries
US20190169604A1 (en) * 2016-08-12 2019-06-06 Board Of Regents, The University Of Texas System Methods and compositions related to barcode assisted ancestral specific expression (baase)
AU2018249444A1 (en) * 2017-04-03 2019-10-03 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for multiplexed quantitative analysis of cell lineages
EP3682004A4 (en) * 2017-09-15 2021-05-26 The Board of Trustees of the Leland Stanford Junior University MULTIPLEX PRODUCTION AND BARCODING OF GENETICALLY MODIFIED CELLS
WO2019084046A1 (en) * 2017-10-23 2019-05-02 The Broad Institute, Inc. ENRICHING SINGLE CELL CELLULAR CONSTITUENT OF BARCODE SEQUENCING LIBRARY
WO2019089803A1 (en) * 2017-10-31 2019-05-09 The Broad Institute, Inc. Methods and compositions for studying cell evolution
WO2019113499A1 (en) * 2017-12-07 2019-06-13 The Broad Institute, Inc. High-throughput methods for identifying gene interactions and networks
WO2019222284A1 (en) * 2018-05-14 2019-11-21 The Broad Institute, Inc. In situ cell screening methods and systems
US11414701B2 (en) * 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US20200283843A1 (en) * 2019-03-04 2020-09-10 The Broad Institute, Inc. Methods and compositions for massively parallel variant and small molecule phenotyping

Also Published As

Publication number Publication date
WO2022192191A1 (en) 2022-09-15
IL305151B1 (en) 2024-09-01
KR20240146111A (ko) 2024-10-07
AU2022232600A1 (en) 2023-09-14
IL305151B2 (en) 2025-01-01
KR20230134617A (ko) 2023-09-21
IL305151A (en) 2023-10-01
BR112023018157A2 (pt) 2023-10-31
KR102809622B1 (ko) 2025-05-19
US20240167020A1 (en) 2024-05-23
KR20240150520A (ko) 2024-10-15
IL314642A (en) 2024-09-01
EP4305164A1 (en) 2024-01-17
JP2024509446A (ja) 2024-03-01
CA3209070A1 (en) 2022-09-22

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