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MX2023005150A - Composiciones y metodos para el reemplazo de alelos con adn codificado por arn. - Google Patents

Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.

Info

Publication number
MX2023005150A
MX2023005150A MX2023005150A MX2023005150A MX2023005150A MX 2023005150 A MX2023005150 A MX 2023005150A MX 2023005150 A MX2023005150 A MX 2023005150A MX 2023005150 A MX2023005150 A MX 2023005150A MX 2023005150 A MX2023005150 A MX 2023005150A
Authority
MX
Mexico
Prior art keywords
methods
alleles
rna
replacement
compositions
Prior art date
Application number
MX2023005150A
Other languages
English (en)
Inventor
Joseph Matthew Watts
Aaron Hummel
Yongjoo Kim
Shai Joshua Lawit
David Schwark
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of MX2023005150A publication Critical patent/MX2023005150A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se refiere a construcciones nucleicas recombinantes que comprenden proteínas efectoras CRISPR-Cas, transcriptasas inversas y ácidos nucleicos guía extendidos y métodos de uso de los mismos para modificar ácidos nucleicos en plantas.
MX2023005150A 2020-11-06 2021-11-05 Composiciones y metodos para el reemplazo de alelos con adn codificado por arn. MX2023005150A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063110386P 2020-11-06 2020-11-06
PCT/US2021/058235 WO2022098993A2 (en) 2020-11-06 2021-11-05 Compositions and methods for rna-encoded dna-replacement of alleles

Publications (1)

Publication Number Publication Date
MX2023005150A true MX2023005150A (es) 2023-05-26

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MX2023005150A MX2023005150A (es) 2020-11-06 2021-11-05 Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.

Country Status (11)

Country Link
US (1) US20220145334A1 (es)
EP (1) EP4240844A2 (es)
JP (1) JP2023549339A (es)
KR (1) KR20230106633A (es)
CN (1) CN116745418A (es)
AU (1) AU2021374974A1 (es)
CA (1) CA3200521A1 (es)
CL (1) CL2023001284A1 (es)
IL (1) IL302526A (es)
MX (1) MX2023005150A (es)
WO (1) WO2022098993A2 (es)

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KR20230106633A (ko) 2023-07-13
US20220145334A1 (en) 2022-05-12
WO2022098993A3 (en) 2022-06-16
WO2022098993A2 (en) 2022-05-12
EP4240844A2 (en) 2023-09-13
IL302526A (en) 2023-07-01
AU2021374974A9 (en) 2025-03-20
CL2023001284A1 (es) 2023-12-22
AU2021374974A1 (en) 2023-05-25
JP2023549339A (ja) 2023-11-24
CN116745418A (zh) 2023-09-12
CA3200521A1 (en) 2022-05-12

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