MX2021008558A - Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos. - Google Patents
Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos.Info
- Publication number
- MX2021008558A MX2021008558A MX2021008558A MX2021008558A MX2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A
- Authority
- MX
- Mexico
- Prior art keywords
- identifying
- fragments
- antibody
- mixed
- molecular weight
- Prior art date
Links
- 238000013467 fragmentation Methods 0.000 title abstract 2
- 238000006062 fragmentation reaction Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 abstract 3
- 230000005526 G1 to G0 transition Effects 0.000 abstract 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 abstract 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000003480 eluent Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/724—Nebulising, aerosol formation or ionisation
- G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
Abstract
Métodos para identificar y cuantificar fragmentos de anticuerpo e identificar el sitio de fragmentación de un anticuerpo en una muestra que comprende las etapas de cargar una muestra que incluye fragmentos del anticuerpo en un sistema cromatográfico que tiene una fase estacionaria (110) de exclusión por tamaño de modo mixto, lavar dicha fase estacionaria de exclusión por tamaño de modo mixto mediante el uso de una fase móvil para proporcionar un eluyente que comprende dichos fragmentos, determinar el peso molecular de dichos fragmentos mediante el uso de un espectrómetro de masas (120), y correlacionar dicho peso molecular con estándares proteicos conocidos.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962793004P | 2019-01-16 | 2019-01-16 | |
| PCT/US2020/013648 WO2020150328A1 (en) | 2019-01-16 | 2020-01-15 | Method and system of identifying and quantifying antibody fragmentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021008558A true MX2021008558A (es) | 2021-08-19 |
Family
ID=69529055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021008558A MX2021008558A (es) | 2019-01-16 | 2020-01-15 | Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos. |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US11486864B2 (es) |
| EP (1) | EP3911947B1 (es) |
| JP (1) | JP2022517338A (es) |
| KR (1) | KR20210116502A (es) |
| CN (1) | CN113227785A (es) |
| AU (1) | AU2020208360B2 (es) |
| BR (1) | BR112021011194A2 (es) |
| CA (1) | CA3123052A1 (es) |
| EA (1) | EA202191961A1 (es) |
| ES (1) | ES3027676T3 (es) |
| IL (1) | IL284637A (es) |
| MX (1) | MX2021008558A (es) |
| SG (1) | SG11202106370WA (es) |
| WO (1) | WO2020150328A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112021008210A2 (pt) | 2018-10-31 | 2021-08-03 | Regeneron Pharmaceuticals, Inc. | método e sistema de identificação e quantificação de uma proteína |
| CN113227785A (zh) | 2019-01-16 | 2021-08-06 | 瑞泽恩制药公司 | 识别和定量抗体片段化的方法和系统 |
| CN115769056A (zh) * | 2020-06-09 | 2023-03-07 | 里珍纳龙药品有限公司 | 用于定量抗体的高通量和基于质谱的方法 |
| EP4370917A1 (en) * | 2021-07-13 | 2024-05-22 | Regeneron Pharmaceuticals, Inc. | Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms) |
| WO2024163240A1 (en) * | 2023-02-01 | 2024-08-08 | Regeneron Pharmaceuticals, Inc. | Characterization of serine-lysine cross-link in antibody high molecular weight species |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE468534T1 (de) * | 2002-08-06 | 2010-06-15 | Univ Johns Hopkins | Verwendung von biomarkern zum nachweis von eierstockkrebs |
| EP1929299B1 (en) * | 2005-09-27 | 2010-12-22 | George Mason Intellectual Properties, Inc. | Method of isolating analytes from a sample |
| CN103858003B (zh) * | 2011-09-29 | 2018-06-22 | 西雅图基因公司 | 蛋白偶联的试剂化合物的整体分子量测定 |
| EP2768857B1 (en) * | 2011-10-19 | 2020-01-01 | NovImmune SA | Methods of purifying antibodies |
| BR112015011317A2 (pt) * | 2012-11-30 | 2017-07-11 | Novartis Ag | métodos para preparar conjugados de proteínas contendo dissulfeto |
| US11092574B2 (en) * | 2013-12-24 | 2021-08-17 | Waters Technologies Corporation | Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides |
| US10119944B2 (en) * | 2013-12-24 | 2018-11-06 | Waters Technologies Corporation | Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides |
| WO2016018978A1 (en) * | 2014-07-29 | 2016-02-04 | Mayo Foundation For Medical Education And Research | Quantifying monoclonal antibody therapeutics by lc-ms/ms |
| US10695693B2 (en) * | 2015-03-13 | 2020-06-30 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
| MX2018009339A (es) * | 2016-02-04 | 2019-01-10 | Oncobiologics Inc | Métodos para la identificación y análisis de secuencias de aminoácidos de las proteínas. |
| WO2018018011A2 (en) * | 2016-07-22 | 2018-01-25 | Amgen Inc. | Methods of purifying fc-containing proteins |
| KR20230041834A (ko) * | 2016-08-16 | 2023-03-24 | 리제너론 파아마슈티컬스, 인크. | 혼합물로부터 개별 항체들을 정량하는 방법 |
| BR112021008210A2 (pt) * | 2018-10-31 | 2021-08-03 | Regeneron Pharmaceuticals, Inc. | método e sistema de identificação e quantificação de uma proteína |
| CN113227785A (zh) | 2019-01-16 | 2021-08-06 | 瑞泽恩制药公司 | 识别和定量抗体片段化的方法和系统 |
| SG11202110968VA (en) * | 2019-12-06 | 2021-10-28 | Regeneron Pharma | Anti-vegf protein compositions and methods for producing the same |
-
2020
- 2020-01-15 CN CN202080007050.6A patent/CN113227785A/zh active Pending
- 2020-01-15 JP JP2021539900A patent/JP2022517338A/ja active Pending
- 2020-01-15 EP EP20704735.8A patent/EP3911947B1/en active Active
- 2020-01-15 CA CA3123052A patent/CA3123052A1/en active Pending
- 2020-01-15 SG SG11202106370WA patent/SG11202106370WA/en unknown
- 2020-01-15 US US16/743,296 patent/US11486864B2/en active Active
- 2020-01-15 MX MX2021008558A patent/MX2021008558A/es unknown
- 2020-01-15 ES ES20704735T patent/ES3027676T3/es active Active
- 2020-01-15 WO PCT/US2020/013648 patent/WO2020150328A1/en not_active Ceased
- 2020-01-15 EA EA202191961A patent/EA202191961A1/ru unknown
- 2020-01-15 AU AU2020208360A patent/AU2020208360B2/en active Active
- 2020-01-15 KR KR1020217024445A patent/KR20210116502A/ko not_active Ceased
- 2020-01-15 BR BR112021011194-8A patent/BR112021011194A2/pt not_active Application Discontinuation
-
2021
- 2021-07-06 IL IL284637A patent/IL284637A/en unknown
-
2022
- 2022-09-23 US US17/951,614 patent/US12000811B2/en active Active
-
2024
- 2024-04-23 US US18/643,509 patent/US12320790B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020208360B2 (en) | 2025-05-08 |
| CA3123052A1 (en) | 2020-07-23 |
| JP2022517338A (ja) | 2022-03-08 |
| US11486864B2 (en) | 2022-11-01 |
| CN113227785A (zh) | 2021-08-06 |
| US12320790B2 (en) | 2025-06-03 |
| US20230314388A1 (en) | 2023-10-05 |
| EP3911947A1 (en) | 2021-11-24 |
| ES3027676T3 (en) | 2025-06-16 |
| KR20210116502A (ko) | 2021-09-27 |
| US12000811B2 (en) | 2024-06-04 |
| IL284637A (en) | 2021-08-31 |
| BR112021011194A2 (pt) | 2021-08-31 |
| US20200225199A1 (en) | 2020-07-16 |
| AU2020208360A1 (en) | 2021-07-01 |
| EP3911947B1 (en) | 2025-04-09 |
| SG11202106370WA (en) | 2021-07-29 |
| WO2020150328A1 (en) | 2020-07-23 |
| US20240272128A1 (en) | 2024-08-15 |
| EA202191961A1 (ru) | 2021-12-13 |
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