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MX2019002376A - Metodos de amplificacion digital del genoma completo. - Google Patents

Metodos de amplificacion digital del genoma completo.

Info

Publication number
MX2019002376A
MX2019002376A MX2019002376A MX2019002376A MX2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A MX 2019002376 A MX2019002376 A MX 2019002376A
Authority
MX
Mexico
Prior art keywords
whole genome
methods
digital amplification
amplification
amplicons
Prior art date
Application number
MX2019002376A
Other languages
English (en)
Inventor
Xing Dong
Sunney Xie Xiaoliang
Han Chang Chi-
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of MX2019002376A publication Critical patent/MX2019002376A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente descripcion proporciona un metodo para la amplificacion del ADN genomico, tal como la amplificacion del genoma completo, incluyendo el uso de un sistema de transposasa para hacer fragmentos del ADN genomico, incluyendo los sitios de union del iniciador, aislar en aceite cada fragmento dentro de su propia microgota acuosa junto con los reactivos de amplificacion de PCR, amplificar cada fragmento dentro de su propia microgota acuosa, demulsificar las microgotas para obtener los amplicones y secuenciar los amplicones.
MX2019002376A 2016-08-31 2016-08-31 Metodos de amplificacion digital del genoma completo. MX2019002376A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/097520 WO2018039969A1 (en) 2016-08-31 2016-08-31 Methods of whole genome digital amplification

Publications (1)

Publication Number Publication Date
MX2019002376A true MX2019002376A (es) 2019-06-20

Family

ID=61299616

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019002376A MX2019002376A (es) 2016-08-31 2016-08-31 Metodos de amplificacion digital del genoma completo.

Country Status (9)

Country Link
US (1) US20210285038A1 (es)
EP (1) EP3507379A4 (es)
JP (1) JP6882453B2 (es)
CN (1) CN109923214A (es)
AU (1) AU2016421196A1 (es)
CA (1) CA3034959A1 (es)
MX (1) MX2019002376A (es)
RU (1) RU2736351C2 (es)
WO (1) WO2018039969A1 (es)

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US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
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WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
EP4219010A1 (en) 2014-04-10 2023-08-02 10X Genomics, Inc. Methods for encapsulating and partitioning reagents
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CA3064709A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
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WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
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US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
JP2023518250A (ja) * 2020-03-20 2023-04-28 ミッション バイオ インコーポレイテッド 全ゲノム増幅のためのシングルセルワークフロー
WO2021222267A1 (en) * 2020-04-28 2021-11-04 President And Fellows Of Harvard College Systems and methods for determining viruses or other pathogens
RU2752840C1 (ru) * 2020-12-17 2021-08-09 Общество с ограниченной ответственностью «ДНК-дисплей» Способ штрихкодирования ампликонов при подготовке таргетных библиотек ДНК к массовому параллельному секвенированию
CN115807058B (zh) * 2022-12-02 2023-06-30 中国科学院水生生物研究所 一种低偏向的单精子全基因组扩增方法
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Also Published As

Publication number Publication date
AU2016421196A1 (en) 2019-03-21
RU2019108270A (ru) 2020-10-01
EP3507379A4 (en) 2020-05-13
CN109923214A (zh) 2019-06-21
EP3507379A1 (en) 2019-07-10
RU2019108270A3 (es) 2020-10-01
WO2018039969A1 (en) 2018-03-08
JP2019531714A (ja) 2019-11-07
RU2736351C2 (ru) 2020-11-16
JP6882453B2 (ja) 2021-06-02
CA3034959A1 (en) 2018-03-08
US20210285038A1 (en) 2021-09-16

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