MX2019000805A - Variantes de serinproteasa y polinucleotidos que las codifican. - Google Patents
Variantes de serinproteasa y polinucleotidos que las codifican.Info
- Publication number
- MX2019000805A MX2019000805A MX2019000805A MX2019000805A MX2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A MX 2019000805 A MX2019000805 A MX 2019000805A
- Authority
- MX
- Mexico
- Prior art keywords
- variants
- protease
- polynucleotides encoding
- serine protease
- increase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Confectionery (AREA)
Abstract
La presente invención se refiere a variantes de proteasa, que presentan una mejora de las propiedades en comparación con la proteasa progenitora, en particular variantes de una serinproteasa que pertenece a la familia 53 obtenida a partir de una cepa de Meripilus giganteus. Las variantes de acuerdo con la invención presentan en particular un incremento de la termoestabilidad, por ejemplo, un incremento de la actividad residual después de 30 min a una temperatura en el intervalo de 55 a 65 °C y/o incremento de la temperatura de desnaturalización térmica, en comparación con la proteasa de Meripilus giganteus progenitora. La presente invención también se refiere a polinucleótidos que codifican las variantes; a constructos de ácido nucleico, vectores y células huésped que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16180497 | 2016-07-21 | ||
| EP16195078 | 2016-10-21 | ||
| PCT/EP2017/067883 WO2018015304A1 (en) | 2016-07-21 | 2017-07-14 | Serine protease variants and polynucleotides encoding same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019000805A true MX2019000805A (es) | 2019-06-20 |
Family
ID=59501396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019000805A MX2019000805A (es) | 2016-07-21 | 2017-07-14 | Variantes de serinproteasa y polinucleotidos que las codifican. |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US10927361B2 (es) |
| EP (1) | EP3487996B1 (es) |
| CN (1) | CN109642225A (es) |
| CA (1) | CA3028875A1 (es) |
| DK (1) | DK3487996T5 (es) |
| ES (1) | ES2981748T3 (es) |
| FI (1) | FI3487996T3 (es) |
| MX (1) | MX2019000805A (es) |
| WO (1) | WO2018015304A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3487996T5 (da) * | 2016-07-21 | 2024-09-02 | Novozymes As | Serinproteasevarianter og polynukleotider, der koder for samme |
| ES2884155T3 (es) | 2016-07-21 | 2021-12-10 | Novozymes As | Variantes de serina proteasa y polinucleótidos que codifican las mismas |
| MX2021011166A (es) * | 2019-03-18 | 2021-10-22 | Novozymes As | Polipeptidos que tienen actividad pululanasa adecuados para usarse en licuefaccion. |
| KR102316445B1 (ko) * | 2019-11-29 | 2021-10-26 | 씨제이제일제당 주식회사 | 신규 세린 프로테아제 변이체 |
| CN112574978B (zh) * | 2021-01-19 | 2021-08-17 | 青岛尚德生物技术有限公司 | 提高降解醇溶蛋白能力的蛋白酶突变体及其编码基因和应用 |
| PE20251535A1 (es) | 2022-05-14 | 2025-06-05 | Novozymes As | Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas |
| WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
| CN120380158A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 用于降低发酵产物生产方法后端的浆体粘度的方法 |
| CN120693401A (zh) | 2022-12-19 | 2025-09-23 | 诺维信公司 | 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途 |
| EP4638725A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024137246A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024258820A2 (en) | 2023-06-13 | 2024-12-19 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
| US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
| US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
| JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| ES2052565T3 (es) | 1986-07-09 | 1994-07-16 | Novo Nordisk As | Un procedimiento para licuar una suspension de almidon o granos amilaceos. |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| JP3016587B2 (ja) | 1989-12-04 | 2000-03-06 | ザ・リサーチ・ファンデーション・オブ・ステート・ユニバーシティ・オブ・ニューヨーク | 非ステロイド抗炎症剤及びテトラサイクリンの配合 |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| EP0765394B1 (en) | 1994-06-03 | 2001-10-04 | Novo Nordisk Biotech, Inc. | Purified myceliophthora laccases and nucleic acids encoding same |
| AU2705895A (en) | 1994-06-30 | 1996-01-25 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| KR20010015754A (ko) | 1997-10-13 | 2001-02-26 | 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 | α-아밀라제 변이체 |
| WO2000024883A1 (en) | 1998-10-26 | 2000-05-04 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
| CN100482801C (zh) | 1999-03-22 | 2009-04-29 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| US7314712B2 (en) | 2001-07-27 | 2008-01-01 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| BR0306740A (pt) | 2002-01-23 | 2004-12-28 | Royal Nedalco B V | Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação |
| CN100564534C (zh) | 2002-02-08 | 2009-12-02 | 金克克国际有限公司 | 以碳底物生产乙醇的方法 |
| WO2004085627A1 (en) | 2003-03-26 | 2004-10-07 | Forskarpatent I Syd Ab | New saccharomyces cerevisiae strains utilizing xylose |
| US7968318B2 (en) | 2006-06-06 | 2011-06-28 | Genencor International, Inc. | Process for conversion of granular starch to ethanol |
| JP5625199B2 (ja) | 2008-03-07 | 2014-11-19 | ディーエスエム アイピー アセッツ ビー．ブイ． | ペントース糖発酵細胞 |
| EP2326723B1 (en) | 2008-08-20 | 2016-07-06 | Novozymes A/S | Processes for producing fermentation products |
| CN102307987B (zh) | 2008-12-24 | 2016-11-16 | 帝斯曼知识产权资产管理有限公司 | 木糖异构酶基因及其在戊糖发酵中的用途 |
| WO2011066576A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| MX2012006176A (es) | 2009-12-01 | 2012-06-25 | Novozymes North America Inc | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. |
| CN102753680B (zh) | 2009-12-11 | 2015-05-13 | 诺维信公司 | 蛋白酶变体 |
| JP5874039B2 (ja) | 2010-11-05 | 2016-03-01 | パナソニックＩｐマネジメント株式会社 | ステレオ画像処理装置及びステレオ画像処理方法 |
| US9732332B2 (en) | 2010-11-08 | 2017-08-15 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| EP2527448A1 (en) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi |
| CA2840962C (en) | 2011-07-06 | 2021-04-13 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
| DK2893012T3 (en) | 2012-09-05 | 2018-12-03 | Novozymes As | Polypeptides with protease activity |
| CA2918685C (en) * | 2013-08-30 | 2024-01-02 | Novozymes A/S | Enzyme composition and uses thereof |
| MX380453B (es) | 2014-06-27 | 2025-03-12 | Dsm Ip Assets Bv | Metodo para mejorar el valor nutricional de pienso para animales. |
| CN108350444A (zh) * | 2015-09-25 | 2018-07-31 | 诺维信公司 | 丝氨酸蛋白酶用于改进乙醇产量的用途 |
| ES2884155T3 (es) * | 2016-07-21 | 2021-12-10 | Novozymes As | Variantes de serina proteasa y polinucleótidos que codifican las mismas |
| DK3487996T5 (da) * | 2016-07-21 | 2024-09-02 | Novozymes As | Serinproteasevarianter og polynukleotider, der koder for samme |
-
2017
- 2017-07-14 DK DK17746005.2T patent/DK3487996T5/da active
- 2017-07-14 FI FIEP17746005.2T patent/FI3487996T3/fi active
- 2017-07-14 CA CA3028875A patent/CA3028875A1/en active Pending
- 2017-07-14 US US16/318,496 patent/US10927361B2/en active Active
- 2017-07-14 WO PCT/EP2017/067883 patent/WO2018015304A1/en not_active Ceased
- 2017-07-14 ES ES17746005T patent/ES2981748T3/es active Active
- 2017-07-14 CN CN201780044163.1A patent/CN109642225A/zh active Pending
- 2017-07-14 MX MX2019000805A patent/MX2019000805A/es unknown
- 2017-07-14 EP EP17746005.2A patent/EP3487996B1/en active Active
-
2021
- 2021-01-14 US US17/148,790 patent/US11447763B2/en active Active
- 2021-12-09 US US17/547,098 patent/US12291730B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3487996A1 (en) | 2019-05-29 |
| EP3487996B1 (en) | 2024-04-10 |
| ES2981748T3 (es) | 2024-10-10 |
| US10927361B2 (en) | 2021-02-23 |
| US20220090041A1 (en) | 2022-03-24 |
| DK3487996T3 (da) | 2024-05-21 |
| US12291730B2 (en) | 2025-05-06 |
| CA3028875A1 (en) | 2018-01-25 |
| US20210139876A1 (en) | 2021-05-13 |
| FI3487996T3 (fi) | 2024-07-10 |
| US11447763B2 (en) | 2022-09-20 |
| CN109642225A (zh) | 2019-04-16 |
| WO2018015304A1 (en) | 2018-01-25 |
| US20190352629A1 (en) | 2019-11-21 |
| DK3487996T5 (da) | 2024-09-02 |
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